CN105613547B - Glibenclamide is being prepared for preventing and treating by the purposes in the bactericide of the microbial plant disease of pathogenic - Google Patents
Glibenclamide is being prepared for preventing and treating by the purposes in the bactericide of the microbial plant disease of pathogenic Download PDFInfo
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- CN105613547B CN105613547B CN201610191592.9A CN201610191592A CN105613547B CN 105613547 B CN105613547 B CN 105613547B CN 201610191592 A CN201610191592 A CN 201610191592A CN 105613547 B CN105613547 B CN 105613547B
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- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
It is used to prevent and treat by the purposes in the bactericide of the microbial plant disease of pathogenic preparing the invention discloses glibenclamide, the present invention passes through Toxicity Determination, it was demonstrated that glibenclamide has plant pathogenic fungi good inhibitory activity.Glibenclamide has the advantages that efficient and low toxicity, is suitable for the requirement of plant disease chemical prevention as bactericide.The use of current bulk sterilization agent, causing the resistance to the action of a drug of pathogen strengthen, and traditional bactericide is big for environment pollution, residual height, directly threatens the food security of the mankind.And glibenclamide is a kind of degradable, pollution-free, environment amenable micromolecular compound, and its resistance to the action of a drug is poor, to non-target organism and person poultry safety, the high-quality of agricultural product and fruits and vegetables is ensure that, meets the requirement of sustainable development, it is studied and market application foreground is wide.
Description
Technical field
The present invention relates to tool Fungicidal active compound, more particularly to glibenclamide is being prepared for preventing and treating by phytopathogen
Purposes in the bactericide of caused plant disease.
Background technology
Into the new century, facing mankind more challenges and crisis, wherein population increase, shortage of resources, environmental pollution,
The problems such as ecological disruption and frequent natural calamity, becomes increasingly conspicuous, seriously hinder agricultural sustainable development, agricultural production also because
This undertakes unprecedented immense pressure.Estimated according to FAO (Food and Agriculture Organization of the United Nation), global grain and Cotton Production are because of disease
Loss is more than 10% throughout the year.Plant disease can not only cause the reduction of crop yield, and also serious to a certain extent
Threaten quality safety and its international trade of agricultural product.
The history of plant disease chemical prevention develops along with the generation of plant disease, the practice and application of chemical prevention
The history in century more than one is had so far.Chemical prevention has the advantages that instant effect, wide sterilization spectrum, cost are low, easy to use, but
Long-term a large amount of uses of bactericide can cause the environmental pollutions such as soil, air, the destruction ecological balance.As people in recent years are to food
The concern of product safety and problem of environmental pollution, some chemical bactericides have been strictly limited in many developed countries and regions to be made
With.People begin look for it is a kind of to the mankind and it is environmentally friendly and with good prevention effect new control of plant disease strategy.
Novel green bactericide is efficient and nontoxic, harmless, pollution-free, do not develop immunity to drugs, and not only conforms with need of the people to pollution-free food
Ask, and the sustainable development for agricultural provides guarantee, it is therefore desirable to have more efficient, low toxicity, low-residual and environment friend
The bactericide of good type is developed and applied, and is played an important role in agricultural production.
Glibenclamide, also known as glibenclamide, chemical name：N- (2- (4- ((((Cyclohexylamino) carbonyl) amino) sulfonyl) benzene
Base) ethyl) -2- methoxyl group -5- chlorobenzamides；English name：Glybenclamide,5-chloro-N-(2-{4-
[(cyclohexylcarbamoyl)sulfamoyl]phenyl}ethyl)-2-methoxybenzam ide；No. CAS：
10238-21-8；Molecular formula：C23H28ClN3O5S；Molecular weight：494.01；Molecular structural formula is：
White crystal powder, it is water insoluble, it is slightly soluble in ethanol, acetone, chloroform.Glibenclamide is used for hypoglycemic agent, uses
Yu Zhong, slight Non-Insulin Dependent Diabetes Mellitus treatment.Glibenclamide is by increasing portal vein insulin level or to liver
Directly act on, suppress hepatic glycogenolytic and gluconeogenesis, liver generation and output glucose are reduced；Oral absorption is fast, albumen
Percentage bound is very high, is 95%, oral rear 2~5 hours blood concentration peakings, continuous action 24 hours.T1/2 is 10 hours.
Intrahepatic metabolism, by liver and kidney discharge each about 50%.
Glibenclamide capsule：Its effect is strong compared with orinase 100 times.Acted within 30 minutes after oral, last about 16
~24 hours.Easily absorbed from intestines and stomach, plasma protein binding rate is high.T1/2 is about 10h.Almost by liver metabolism, main generation
Thank to thing hypoglycemic activity weaker.About 50% metabolin is drained from urine, 50% is discharged into excrement through bile in addition.Glibenclamide Tablets：Should
Product are hypoglycemic agent.(1) stimulating pancreas islet β cell insulin, prerequisite is that beta Cell of islet also has certain conjunction
Into the function with excreting insulin；(2) is by increasing portal vein insulin level or to the direct effect of liver, suppressing hepatic glycogen
Decompose and gluconeogenesis, liver generation and output glucose are reduced；(3) may also increase outside pancreas and organize to the quick of insulin
The utilization (may be mainly by being acted on after acceptor) of perception and sugar, therefore, total effect is reduction fasting blood-glucose and postprandial blood sugar.
The product are white tablets.
At present, the report to agriculture pathogen inhibitory action on glibenclamide is there is no both at home and abroad.
The content of the invention
Prepared object of the present invention is to provide glibenclamide for preventing and treating by the microbial phytopathy of pathogenic
Purposes in harmful bactericide.The present invention passes through toxicity test, it was demonstrated that glibenclamide has good to plant pathogenic fungi
Inhibitory activity.Glibenclamide is suitable for the requirement of plant disease chemical prevention as bactericide, its efficient, low toxicity.
For achieving the above object, the present invention is achieved using following technical scheme：
Prepared the invention provides glibenclamide for preventing and treating the bactericide by the microbial plant disease of pathogenic
In purposes.
Further：The phytopathogen is that Deuteromycotina fungi, Ascomycotina fungi and Mastigomycotina are true
Bacterium.
Further：Effective concentration of glibenclamide is 5-10mM in the bactericide.
Further：The Deuteromycotina fungi includes blueberry and intends stem point branch-rot bacterium, tomato wilt bacterium, tomato morning
Epidemic disease bacterium, blueberry branch-rot bacterium, tobacco brown spot pathogen, rice blast fungus, fusarium graminearum, cucumber rod spore leaf spot fungi,
Verticillium dahliae, potato wilt bacterium, Botrytis cinerea, Ustilaginoidea virens, Tobacco target spot bacterium, the cercospora black spot of peanut
Bacterium and Maize Curvularia.
Further：The Ascomycotina fungi includes blueberry limb ulcer bacteria, Botryosphaeria berengeriana f. sp, apple decay
Germ and pears rotten pathogenic bacteria.
Further：The Mastigomycotina fungi includes camphor tree phytophthora germ and tobacco black shank bacterium.
Further：When the concentration of glibenclamide is 10mM in the bactericide, glibenclamide is to botrytis cinerea, indigo plant
The inhibiting rate of 7 kinds of phytopathogens such as certain kind of berries limb ulcer bacteria and Botryosphaeria berengeriana f. sp is up to 100%.
Further：The plant is camphor tree, apple, peach, pears, tomato, blueberry, grape, tobacco, cucumber, cotton, water
Rice, corn, wheat, peanut and soybean.
Compared with prior art, advantages of the present invention and have the technical effect that：The kind mechanism of action of current bactericide compares
Single, the reproduction speed of pathogen is very fast, therefore resistance is also produced comparatively fast, and pollution is big, high residue, threatens the food peace of the mankind
Entirely.
The present invention passes through Toxicity Determination, it was demonstrated that there is to plant pathogenic fungi glibenclamide good suppression to live
Property.Glibenclamide has the advantages that efficient and low toxicity, is suitable for the requirement of plant disease chemical prevention as bactericide.At present
The use of bulk sterilization agent, causing the resistance to the action of a drug of pathogen strengthens, and traditional bactericide is big for environment pollution, residual is high,
Directly threaten the food security of the mankind.As people increasingly improve to pollution-free, pollution-free green food cry, green is killed
Microbial inoculum turns into the important prevention and controls in agricultural production, and many green bactericides have been come out one after another, and is widely used in producing,
Achieve remarkable result.And glibenclamide is a kind of degradable, pollution-free, environment amenable micromolecular compound, and its
The resistance to the action of a drug is poor, to non-target organism and person poultry safety, ensure that the high-quality of agricultural product and fruits and vegetables, meets sustainable development
It is required that, it is studied and market application foreground is wide.
Brief description of the drawings
Fig. 1 is the bacteriostatic experiment result that glibenclamide intends blueberry stem point branch-rot bacterium in the present invention；
Fig. 2 is bacteriostatic experiment result of the glibenclamide to tomato early blight bacterium in the present invention；
Fig. 3 is bacteriostatic experiment result of the glibenclamide to tobacco brown spot pathogen in the present invention；
Fig. 4 is bacteriostatic experiment result of the glibenclamide to botrytis cinerea in the present invention；
Fig. 5 is bacteriostatic experiment result of the glibenclamide to fusarium graminearum in the present invention；
Fig. 6 be in the present invention glibenclamide to potato verticillium wilt pathogen bacteriostatic experiment result；
Fig. 7 is bacteriostatic experiment result of the glibenclamide to Tobacco target spot bacterium in the present invention；
Fig. 8 is bacteriostatic experiment result of the glibenclamide to Maize Curvularia in the present invention；
Fig. 9 is bacteriostatic experiment result of the glibenclamide to cucumber rod spore leaf spot fungi in the present invention；
Figure 10 is bacteriostatic experiment result of the glibenclamide to blueberry branch-rot bacterium in the present invention；
Figure 11 is bacteriostatic experiment result of the glibenclamide to cotton-wilt fusarium in the present invention；
Figure 12 is bacteriostatic experiment result of the glibenclamide to Chinese cabbage verticillium wilt pathogen in the present invention；
Figure 13 is bacteriostatic experiment result of the glibenclamide to apple anthrax leaf blight in the present invention；
Figure 14 is bacteriostatic experiment result of the glibenclamide to blueberry limb ulcer bacteria in the present invention；
Figure 15 is bacteriostatic experiment result of the glibenclamide to Valsa mali in the present invention；
Figure 16 is bacteriostatic experiment result of the glibenclamide to Botryosphaeria berengeriana f. sp in the present invention；
Figure 17 is bacteriostatic experiment result of the glibenclamide to pears rotten pathogenic bacteria in the present invention；
Figure 18 is bacteriostatic experiment result of the glibenclamide to camphor tree phytophthora germ in the present invention；
Figure 19 is bacteriostatic experiment result of the glibenclamide to tobacco black shank bacterium in the present invention；
Embodiment
Technical scheme is described in further detail below in conjunction with the drawings and specific embodiments.
Embodiment 1
First, experiment material
Glibenclamide is the finished product bought from Sigma companies.5g glibenclamides are weighed in 100ml dimethyl sulfoxide (DMSO),
100M mother liquor is made into, 4 DEG C of preservations of filtration sterilization are stand-by.
Phytopathogen used is the strain of the 4 DEG C of preservations in laboratory in this experiment, and the culture medium used is potato training
Support base (abbreviation PDA).
PDA culture medium is formulated：Potato (peeling) 200g, glucose 20g, agar 15g, running water 1000ml, natural PH
Compound method：Potato is cleaned into peeling, claims 200g to be cut into small pieces, add water well-done (boil 20-30 minutes, energy quilt
Glass bar is poked), with eight layers of filtered through gauze in beaker, needed according to experiment plus 15-20g agar, add 20g grapes
Sugar, stirs, and fully slightly cooling supplies water to 1000ml after dissolving, and 121 DEG C sterilize 20 minutes after packing, standby after cooling.
2nd, experimental method
Using growth rate method.
1st, first by 19 kinds of phytopathogens, 25 DEG C of culture 6d or so are stand-by on PDA plate.
2nd, PDA culture medium heating is dissolved, is cooled to 45-50 DEG C, the glibenclamide for being separately added into various concentrations is made and contained
0th, 2.5, the 5, culture medium of 10mM decoctions, and pour into culture dish and cool down respectively.
3rd, with sterile working formality, each bacterial strain mycelia edge (upgrowth situation is tried one's best unanimously) with card punch in culture 6d is beaten
Circular bacteria cake (diameter 0.60cm) is taken, then is chosen with transfer needle to pastille flat board center, culture dish is then inverted in incubator (25
DEG C) middle culture.
4th, in the growing state of different time Observe and measure mycelia after processing, and measure diameter using crossing method and locate
Data are managed, inhibiting rate is calculated and takes pictures.
Inhibiting rate (%)=(control hyphal diameter-processing hyphal diameter)/control hyphal diameter × 100
Each processing is repeated 3 times.
3rd, fungistatic effect of the glibenclamide to 19 kinds of phytopathogen mycelial growths
1. potato verticillium wilt pathogen：The pathogen for causing cotton verticillium wilt is the verticillium dahliae of Verticillium dahliae category.The cause of disease
The host range of bacterium is extremely wide, and foreign countries report can cause harm 38 section, 660 kinds of plants, wherein 184 kinds of crops, 153 kinds of weeds.China passes through
Identification, host plant at least 80 kinds of 20 section, field crop include cotton, sunflower, eggplant, spicy, tomato, tobacco, potato,
Muskmelon, watermelon, cucumber, peanut, Kidney bean, mung bean, soybean, sesame, beet etc..The germ can make host plant produce verticillium wilt,
Worldwide cause great economic loss.Verticillium wilt preventing and treating is difficult, and such as biological control of existing means of prevention, chemistry are anti-
Control, conventional genetic breeding etc. is all unable to reach preferable prevention effect.
2. tobacco black shank bacterium：The germ can endanger all cultivation cigarettes such as flue-cured tobacco, air-curing of tobacco leaves, suncured tabacco, Turkish tobaccos, burley tobaccos
Grass, causes tobacco black shank.In temperature on average up to 22 DEG C, rainy time and low-lying humid area, tobacco black shank spread
Quickly, it can destroy whole vega in 1~2 week, it is destructive extremely strong, the whole strain of cigarette strain is often resulted in after field infestation dead.Tobacco is black
Shin disease is a kind of destructive soil-borne disease, is also one of global Main Tobacco Diseases.Once occurring, if not in production
Processing, will result in serious economic loss in time.The host range of the germ is narrower, tobacco be its under field conditions (factors) only
One host.Although host range is larger under artificial inoculation conditions, tomato seedling and fruit, the cotton of cotton are also only infected
A few plant such as bell, the stem of papaw and potato, eggplant, capsicum, castor-oil plant, apple, striped cucurbit.
3. Chinese cabbage verticillium wilt pathogen：Early growth period is all gone well.After 5 days, yellow is very swift and violent, the complete a piece of golden yellow, balling in field
Phase outward appearance yellow, root canal blacking be this Disease Characters, can infecting again by soft corruption etc., cause softening and it is withered, but itself
Do not cause softening and withered.Leaf-head is unable to core balling, siphonal lobe shrinkage.Mild person, yield is also greatly influenceed even if balling.
Cause of disease：Survived before sick under relatively low temperature, the small sclerotium of black coaly is formed in the withered siphonal lobe of basal part of stem and middle arteries top.Disease
Bacterium is invaded via soil infection by Chinese cabbage root, infects the conduit of rhizome.Germ is together remained in soil with old complaint, is turned into
The source of infection in the coming year.Germ is hygrophilous not to like drying.Prevention and controls：Often send out in continuous cropping plot.Initially by slope bottom, depression and soil
The high site morbidity of earth moisture, over time, spreads to complete four.All kinds are all susceptible, but the deep broad-leaved type of leaf color
Kind is aggrieved lighter.Early diagnosis.Other crops, not continuous cropping, sterilization soil block are changed, is effectively preventing measure.After harvest
The residual stem of undesirable root, should clear up and burn in time.
4. tomato early blight bacterium：The disease may also suppress tomato growth and fruit formed in addition to directly harm stem, leaf, fruit, reveal
Ground and protecting field are aggrieved all heavier, long-term underproduction 20%-30%, up to more than 50% when serious, or even total crop failure.This cause of disease host
It is in extensive range, in addition to tomato is endangered, it can also endanger the solanaceous vegetable crop such as eggplant, capsicum and potato.
5. fusarium graminearum：Head blight is a kind of destructive disease, and fringe can be caused rotten, causes Severe Reduction and quality to drop
It is low.With the change of global warming, cropping system and mode, wheat scab constantly spreads extension, often results in wheat
The underproduction, quality reduction, and contain mycotoxin in the wheat seed infected, person poultry poisoning and serious disease can be caused.The disease
Bacterium is in addition to wheat is endangered, moreover it is possible to infect the grass family such as a variety of gramineous crops such as barley, oat, paddy rice, corn and goose hat grass
Weeds, in addition, can also infect the crops such as soybean, cotton, sweet potato.
6. Valsa mali：Apple tree canker is that apple tree is main, most serious trunk disease.The disease is except invading
Fruit can also be infected outside dye limb, deadwood, dead tree is caused, causes to ruin garden when serious, so as to cause serious yield and economy
Loss.Apple rot pathogen possesses the extensive host plant of comparison, endangers normal including apple, Lou Tao, peach and pears etc.
See fruit tree.
7. cotton-wilt fusarium：Fusarium oxysporum is the Main Pathogenic Bacteria of potato wilt, and the bacterium is a kind of generation
The soil-borne disease fungal pathogenses of criticality distribution, host range is quite varied, can cause Solanaceae, melon, banana, pulse family, cotton and flowers etc.
The generation of various plants droop.After host is infected by Fusarium oxysporum, Symptoms are varied, generally result in vascular bundle brown
Become, plant wilting withered, bulb and root rot, plant strain growth is weak etc..Potato wilt is that potato is caused in recent years
A kind of important fungoid soil-borne disease of harm, has occurred in many areas, especially in potato weight weak ground occurrence degree
It is even more serious, it directly affects potato yield and its economic benefit.
8. apple anthrax leaf spoting bacteria：Apple anthrax leaf blight is a kind of leaf diseases as caused by anthrax bacteria, mainly
The kinds such as loud, high-pitched sound drawing, Jin Guan, Qiao Najin, Qin Guan of apple are endangered, causes apple tree largely to fall leaves with fruit and seriously rots, tree vigo(u)r
Seriously undermine, cause next year to be had no harvest.Fuji, red magnitude kind are highly disease-resistant, and the loud, high-pitched sound of morbidity is drawn and the adjacent Fuji's apple do not fallen ill
Fruit tree contrasts clearly in orchard.Apple anthrax-bacilus leaf blight main harm blade, and cause a large amount of fallen leaves.
9. blueberry branch-rot bacterium：The germ belongs to plan Pestalotia, is a kind of important phytopathogen, can be parasitic big
The symptoms such as tikka, rotten, ulcer are shown as after the plant of Yue Wushiduo sections, hazard of plant, the yield and product of product is had a strong impact on
Matter.Therefore, production of the pestalotia bacteria to farm-forestry crop is intended in research has important economic implications.At present, the blueberry in production
Main breed, Ru Lanfeng, duke and blue beauty etc., there is branch-rot, significantly affect its fruit yield and quality.
10. tobacco brown spot pathogen：Alternaria alternate is the fungal disease for endangering China's tobacco leaf production, can not only make tobacco leaf
Quality Down and blade incompleteness are damaged, moreover it is possible to reduce its industrial application value, susceptible tobacco leaf is because tobacco leaf ingredient differs and makes cigarette
The mouthfeel of grass declines.Alternaria altemata host range is wider, in addition to tobacco, can also infect cotton, peanut, soybean, tomato, peach,
The various plants such as Lee, wheat, cause the symptoms such as spot, root rot.
11. cucumber rod spore leaf spot fungi：Extensively, circulation way is various for the germ host range.It further investigation reveals that in recent years,
The leaf spot of the vegetables such as cucumber, tomato as caused by cucumber rod spore leaf spot fungi is in the Shandong of China, Hebei, Liaoning, the Inner Mongol
Occur Deng 11 province, city and region's large area, cause serious economic loss.
12. blueberry intends stem point branch-rot bacterium：It is a kind of important trunk disease on blueberry that blueberry, which intends stem point branch-rot,.Host
Scope is wider, mainly there is the plants such as Larch, peach, pear, Vaccinium, Jianshi maple.
13. Botryosphaeria berengeriana f. sp：Ring rot of apple also known as scurf, wheel line decayed fruit disease, are on apple limb portion and fruit
Important biomolecule disaster, often cause that apple limb bark is coarse, locality is downright bad and fruit rot.Ill plant fruit-setting rate is low,
Cause tree body weak and yield is reduced, or even garden is ruined in total crop failure.In recent years, with the commerial growing of susceptible kind Fuji apple,
The ring rot of apple incidence of disease increases year by year, and hazard area constantly expands, it has also become the serious plant disease in Chinese apple production, seriously
Threaten the sustainable development of Apple Industry.
14. pears rotten pathogenic bacteria：Pears rot also known as bark rot, the major branch and side shoot of main harm pear tree, cause pear tree gesture
It is weak, fruit yield and quality decline.The disease has that generation area is wide, the incidence of disease is high, the characteristics of be difficult to control to.In China pears master
There is generation in producing region, occurs especially with Xinjiang, northwest, North China, northeast and other places serious.The serious theatre of morbidity, tree body disease scar is innumerable,
Limb is incomplete, causes a large amount of Si Shuhuohui gardens.
15. blueberry limb ulcer bacteria：Pathogen-the Botryosphaeria for causing blueberry limb canker is a class generation
The fungi of criticality distribution, caused Tree resistance is the great disease occurred in world wide.The host that it endangers is very wide,
Up to 45 category (40 category of broad leaf tree, 5 category of coniferous tree), serious is endangered to woods fruit tree, eucalyptus, willow, apple, stone can be caused
The symptoms such as limb ulcer withered death, dry rot, the gummosis of the host plants such as pomegranate, peach, Cassia occidentalis, red bayberry, Chinese chestnut.
16. Botrytis cinerea：Grape grey mould is the Common Diseases in the microbial grape production of Botrytis cinerea, is also
One of maximum disease is endangered, is occurred universal.The disease occurs particularly in south China area and northern protecting field grape production
Seriously, oneself turns into the Major Diseases of limitation northern China facility culture grapes industry development.Ruining in the disease or storage
Venereal disease of going out does harm to, very big on yield and quality influence, and the grape post-harvest loss caused by gray mold is 20% -30% or so every year,
Cause huge economic loss.The germ is a kind of host range very wide facultative parasite, can infect various fruits, vegetables
And flowers.
17. camphor tree phytophthora germ：Camphor tree phytophthora be also known as brown phytophthora, Chinese cassia tree epidemic disease poison, can cause cinnamon butt rot and
Limb ulcer etc., causes extreme loss.In China, camphor tree phytophthora can also cause deodar in addition to the phytophthora root rot for causing cinnamon
Phytophthora rot.The germ is distributed widely in the areas such as America, European and East Asia, South Asia and Oceania, can infect including woods
Wood and crops including more than 3000 various plants, be one of important pathogen of forest disease control.
18. Maize Curvularia：Maize Curvularia Leaf Spot Disease is also known as plan eye spot, black points, and mainly cause harm maize leaves
Piece, also endangers leaf sheath and bract sometimes, and the change of its symptom is very big in different cultivars.The germ host range is wider, can post
On the fruit for being born in tomato, capsicum, it can also colonize on paddy rice, sorghum, wheat and some lawns so that these plants occur
Disease.Maize Curvularia Leaf Spot Disease is a kind of disease that China North China, northeast corn producing region generally occur in recent years, because the disease is
Sudden disease, is spread rapidly, and leaf portion scab is intensive in flakes when serious, and grave illness plot diseased plant rate and sick leaf rate are up to
100%, it has also become one of the important disease in China's corn producing region.
19. Tobacco target spot bacterium：Tobacco target spot is that, by the microbial tikka class disease of miliary damping-off, the germ is mainly endangered
Evil blade formation scab, scab necrosis part it is frangible formed perforation, occur it is serious when scab in flakes, and plant disease epidemic speed compared with
It hurry up, therefore serious threat constituted to tobacco leaf production.The host range of the germ extensively, can not only infect tobacco, in nature
In the case of can infect cereal crops and beet, the eggplants such as 200 various plants, including corn, sorghum, cotton, wheat class, paddy rice, soybean
Son, tomato, black nightshade, honor fiber crops and petunia etc..In addition, the germ can infect 188 kinds of plants of 32 sections under artificial inoculation conditions
Thing.
In vitro bacteriostasis life survey of the glibenclamide to 19 kinds of phytopathogens the results are shown in Table 1.
Inhibitory action measurement result (6d) of the glibenclamide of table 1 to 19 kinds of pathogen mycelial growths
As can be known from Table 1, with the rise of glibenclamide concentration, its inhibition to 19 kinds of phytopathogens also has not
With the raising of degree.When glibenclamide concentration be 5mM when, to tomato early blight bacterium, botrytis cinerea, camphor tree phytophthora germ,
Tobacco black shank bacterium has good inhibition, and inhibiting rate reaches more than 90%；When glibenclamide concentration is 10mM, its is right
19 kinds of phytopathogens of Deuteromycotina, Ascomycotina and Mastigomycotina have good inhibition, and inhibiting rate is equal
More than 98%.Therefore, as drug concentration gradually rises, inhibiting rate is more obvious, and even up to 100%.
Glibenclamide as shown in figures 1-19, works as glibenclamide to the fungistatic effect pictures of 19 kinds of phytopathogen mycelial growths
Concentration in 10mM, 19 kinds of bacterium all do not grow, and illustrate that glibenclamide, in 10mM, plays good inhibition.To sum up
It is described, in the glibenclamide very potential preventing and treating applied to phytopathogen.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than is limited；Although with reference to foregoing reality
Apply example the present invention is described in detail, for the person of ordinary skill of the art, can still implement to foregoing
Technical scheme described in example is modified, or carries out equivalent substitution to which part technical characteristic；And these are changed or replaced
Change, the essence of appropriate technical solution is departed from the spirit and scope of claimed technical solution of the invention.
Claims (4)
1. glibenclamide is being prepared for preventing and treating by the purposes in the bactericide of the microbial plant disease of pathogenic, its feature
It is：The phytopathogen is Deuteromycotina fungi, Ascomycotina fungi and Mastigomycotina fungi；
The Deuteromycotina fungi includes blueberry and intends stem point branch-rot bacterium, tomato wilt bacterium, tomato early blight bacterium, blueberry branch
Rot bacterium, tobacco brown spot pathogen, rice blast fungus, fusarium graminearum, cucumber rod spore leaf spot fungi, verticillium dahliae,
Potato wilt bacterium, Botrytis cinerea, Ustilaginoidea virens, Tobacco target spot bacterium, cercospora black spot of peanut bacterium and Curvularia
Leaf spot fungi；
The Ascomycotina fungi includes blueberry limb ulcer bacteria, Botryosphaeria berengeriana f. sp, Valsa mali and pears and rotted
Germ；
The Mastigomycotina fungi includes camphor tree phytophthora germ and tobacco black shank bacterium.
2. glibenclamide according to claim 1 is being prepared for preventing and treating killing by the microbial plant disease of pathogenic
Purposes in microbial inoculum, it is characterised in that：Effective concentration of glibenclamide is 5-10 mM in the bactericide.
3. glibenclamide according to claim 1 is being prepared for preventing and treating killing by the microbial plant disease of pathogenic
Purposes in microbial inoculum, it is characterised in that：When the concentration of glibenclamide is 10mM in the bactericide, glibenclamide is to tomato gray mould
The inhibiting rate of germ, blueberry limb ulcer bacteria and Botryosphaeria berengeriana f. sp phytopathogen is up to 100%.
4. glibenclamide according to claim 1 is being prepared for preventing and treating killing by the microbial plant disease of pathogenic
Purposes in microbial inoculum, it is characterised in that：The plant is camphor tree, apple, peach, pears, tomato, blueberry, grape, tobacco, cucumber, cotton
Flower, paddy rice, corn, wheat, peanut and soybean.
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