CN109645007A - C14H10Cl2NNaO2 is in preparation for preventing and treating the application in the fungicide by the microbial plant disease of pathogenic - Google Patents
C14H10Cl2NNaO2 is in preparation for preventing and treating the application in the fungicide by the microbial plant disease of pathogenic Download PDFInfo
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Abstract
The invention discloses C14H10Cl2NNaO2s to pass through indoor toxicity test for prevent and treat the application in fungicide by the microbial plant disease of pathogenic, the present invention in preparation, it was demonstrated that C14H10Cl2NNaO2 is to plant pathogenic fungi with good inhibitory activity.Present invention firstly provides using C14H10Cl2NNaO2 as fungicide, have the advantages that low toxicity and efficient, the requirement suitable for plant disease chemical prevention.Traditional chemical bactericide is big to the pollution of environment, residual is high, food-safe to seriously threaten.C14H10Cl2NNaO2 is a kind of environmental-friendly small molecule compound, it is degradable, pollution-free, and the drug resistance of plant pathogenic fungi it is low, to non-target organism and safety of human and livestock, can guarantee the high-quality of agricultural product and fruits and vegetables, meet the requirement of sustainable development, research and market application prospect are wide.
Description
Technical field
The invention belongs to agricultural biological technical fields, are related to Fungicidal active compound, in particular to C14H10Cl2NNaO2 is being made
Prevention and treatment is ready for use on by the application in the fungicide of the microbial plant disease of pathogenic.
Background technique
Into the new century, the problems such as shortage of resources, environmental pollution, ecological disruption and frequent natural calamity, becomes increasingly conspicuous,
The sustainable development of agricultural is seriously hindered, therefore agricultural production also undertakes unprecedented immense pressure.According to the United Nations's grain
The estimation of agriculture tissue, global grain and Cotton Production are lost throughout the year because of disease 10% or more.Plant disease can not only cause
The reduction of crop yield, and the quality safety and its international trade of agricultural product are also seriously threatened to a certain extent.
Currently, the maximally efficient of prevention and treatment to plant disease is chemical prevention.But it largely is easy to make using chemicals
At Pesticide Residue and the resistance problems of disease fungus, and ecological environment is polluted.Therefore, it is necessary to develop
Efficiently, low toxicity, low-residual and environmentally friendly fungicide, and be applied in production practices.China's agricultural will be towards strong
The problems such as sustainable direction of health is developed, and solves resistance, enhances plant immune ability, is safe and efficient, is good with environment compatibility is
Direction as the development of fungicide product.
C14H10Cl2NNaO2, Chinese nickname: 2- [(2,6- dichlorophenyl) amino]-sodium phenylacetate, dichlorophen acid, diclofenac sodium
Sodium, diclofenac sodium, diclofenac sodium, Diclofenac;English name: Diclofenac sodium salt;
English alias: 2- [(2,6-Dichlorophenyl) amino] benzeneacetic acid sodium salt;No. CAS:
15307-79-6;Molecular formula: C14H10Cl2NNaO2;Molecular weight: 318.13.Molecular structural formula are as follows:
C14H10Cl2NNaO2, white crystalline powder is a kind of non-steroid anti-inflammatory drug (NSAID), Pharmaceutical name take his spirit,
A meter Lei Er etc..The basis of C14H10Cl2NNaO2 analgesia and anti-inflammatory effect is to inhibit cyclooxygenase (COX) inhibitor.C14H10Cl2NNaO2
Also have the function of removing free radical, and the DNA recovery by radiation injury is made to have protective effect, is that a kind of potentially radiate is protected
Protect agent.C14H10Cl2NNaO2 is generally used for alleviating rheumatoid arthritis, osteoarthritis, SpA, urarthritis, rheumatism
The various arthritic painful swelling of joints symptoms such as property arthritis;For treating non-arthrosis various soft tissue rheumatic pains, such as
Injury pain etc. after shoulder pain, tenosynovitis, bursal synovitis, myalgia and movement;Such as acute light, moderate pain: after operation, wound
After wound, after strain, dysmenorrhea, toothache, headache etc.;There is certain refrigeration function to fever.Due to using concentration extremely low, usually make
It is safe and non-toxic under the conditions of.
Currently, there is no the report about C14H10Cl2NNaO2 to agricultural plant pathogen inhibiting effect both at home and abroad.
Summary of the invention
The object of the present invention is to provide C14H10Cl2NNaO2 in preparation for preventing and treating by the microbial plant of pathogenic
Application in the fungicide of disease.The present invention passes through toxicity test, it was demonstrated that C14H10Cl2NNaO2 has plant pathogenic fungi good
Good inhibitory activity.C14H10Cl2NNaO2 is suitable for the requirement of plant disease chemical prevention as fungicide, efficient, low toxicity.
For achieving the above object, the present invention is achieved by the following scheme:
The present invention provides C14H10Cl2NNaO2 in preparation for preventing and treating by the sterilization of the microbial plant disease of pathogenic
Application in agent.
Further: the phytopathogen is Deuteromycotina fungi and Ascomycotina fungi.
Further: the effective of C14H10Cl2NNaO2 uses concentration for 0.5mM-1.5mM in the fungicide.
Further: the Deuteromycotina fungi includes: that the quasi- stem of blueberry selects branch-rot bacterium, blueberry branch-rot bacterium, peanut
Alternaria, tomato early blight bacterium, botrytis cinerea, fusarium graminearum, potato wilt bacterium, Tobacco target spot bacterium,
Verticillium dahliae, Maize Curvularia, cucumber stick spore leaf spot fungi.
Further: the Ascomycotina fungi includes: apple anthrax leaf spoting bacteria, blueberry limb ulcer bacteria, apple
Fruit rotten pathogenic bacteria, Botryosphaeria berengeriana f. sp, pears rotten pathogenic bacteria.
Further: when the concentration of C14H10Cl2NNaO2 is 1.5mM in the fungicide, C14H10Cl2NNaO2 intends stem point to blueberry
Branch-rot bacterium, cercospora black spot of peanut bacterium, Tobacco target spot bacterium, verticillium dahliae, cucumber stick spore leaf spot fungi, apple anthrax leaf are withered
Germ, Botryosphaeria berengeriana f. sp, blueberry limb ulcer bacteria and Valsa mali inhibiting rate be more than 90%.
Further: the plant is apple, pears, tomato, blueberry, tobacco, cucumber, cotton, corn, wheat, potato
And peanut.
Compared with prior art, advantages of the present invention and have the technical effect that at present prevention and treatment phytopathogen mainly by pesticide.
The kind mechanism of action of agriculture chemicals fungicide is relatively simple, and lavishment pesticide brings " 3R " problem, i.e. harmful organism drug resistance
(Resistanee), harmful organism is ferocious again sticks up (Resurgenee) and pesticide residue (Residue), directly threatens the ring of the mankind
Border and food safety.Therefore, exploitation is the important prevention and treatment being had become in agricultural production using pollution-free, nuisanceless green fungicide
Method.Many green bactericides have been come out one after another, and are widely used in producing, and achieve remarkable result.The present invention passes through interior
Toxicity test, it was demonstrated that C14H10Cl2NNaO2 has good inhibitory activity to plant pathogenic fungi.And C14H10Cl2NNaO2 is one
Degradable, pollution-free, the environment amenable small molecule compound of kind, and its drug resistance is poor, pacifies to non-target organism and people and animals
Entirely, the high-quality that can guarantee agricultural product and fruits and vegetables, meets the requirement of sustainable development, and research and market application prospect are wide
It is wealthy.
Detailed description of the invention
Fig. 1 is the bacteriostatic experiment result that C14H10Cl2NNaO2 intends blueberry stem point branch-rot bacterium in the present invention;
Fig. 2 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of blueberry branch-rot bacterium;
Fig. 3 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of cercospora black spot of peanut bacterium;
Fig. 4 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of tomato early blight bacterium;
Fig. 5 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of botrytis cinerea;
Fig. 6 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of fusarium graminearum;
Fig. 7 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of potato wilt bacterium;
Fig. 8 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of Tobacco target spot bacterium;
Fig. 9 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of verticillium dahliae;
Figure 10 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of Maize Curvularia;
Figure 11 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of cucumber stick spore leaf spot fungi;
Figure 12 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of apple anthrax leaf spoting bacteria;
Figure 13 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of pears rotten pathogenic bacteria;
Figure 14 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of Botryosphaeria berengeriana f. sp;
Figure 15 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of blueberry limb ulcer bacteria;
Figure 16 be in the present invention C14H10Cl2NNaO2 to the bacteriostatic experiment result of Valsa mali;
Specific embodiment
Technical solution of the present invention is described in further detail below in conjunction with the drawings and specific embodiments.
Embodiment 1
One, experimental material
C14H10Cl2NNaO2 purchase from Sigma company.3.18g C14H10Cl2NNaO2 is weighed in the dimethyl sulfoxide of 100ml,
It is made into the mother liquor of 0.1M, 4 DEG C of filtration sterilization save for use.
Phytopathogen is stored in 4 DEG C of refrigerators in this experiment, and the culture medium used is potato dextrose medium (abbreviation
PDA)。
PDA culture medium formula: potato (peeling) 200g, glucose 20g, agar 15g, tap water 1000ml, natural pH.
Preparation method: potato is cleaned into peeling, claims 200g to be cut into small pieces, adds boiling rotten (boiling 15-20 minutes), with eight
Layer filtered through gauze needs according to experiment in beaker plus 15-20g agar, addition 20g glucose stirs evenly, after completely dissolution
Slightly cooling water of supplying is to 1000ml, and 121 DEG C sterilizing 20 minutes, spare after cooling after packing.
Two, experimental method
Using growth rate method.
1, first by 16 kinds of phytopathogens, 25 DEG C of culture 6d or so are stand-by on PDA plate.
2, PDA culture medium heating is dissolved, is cooled to 45-50 DEG C, the C14H10Cl2NNaO2 for being separately added into various concentration is made
The culture medium of the medical fluid containing 0,0.5,1.0 and 1.5mM, and pour into respectively cooling in culture dish.
3, it with sterile working formality, is beaten with punch at each bacterial strain mycelia edge (upgrowth situation is consistent as far as possible) of culture 6d
Round bacteria cake (diameter 0.60cm) is taken, then is chosen with transfer needle to drug containing tablet center, culture dish is then inverted in incubator (25
DEG C) in culture.
4, after processing different time Observe and measure mycelia growing state, and measure diameter and locate using crossing method
Data are managed, inhibiting rate is calculated and are taken pictures.
Inhibiting rate (%)=(control hyphal diameter-processing hyphal diameter)/control hyphal diameter × 100;
Each processing is repeated 3 times.
Three, the fungistatic effect that C14H10Cl2NNaO2 grows 16 kinds of phytopathogen mycelia
1. blueberry intends stem point branch-rot bacterium: it is a kind of important trunk disease on blueberry that blueberry, which intends stem point branch-rot,.Host
Range is wider, mainly there is the plants such as Larch, peach, pear, Vaccinium, Jianshi maple.
2. blueberry branch-rot bacterium: the germ belongs to quasi- Pestalotia, is a kind of important phytopathogen, can be parasitic big
The plant of Yue Wushiduo section shows as tikka after hazard of plant, rots, the symptoms such as ulcer, seriously affects the yield and product of product
Matter.Therefore, studying quasi- pestalotia bacteria has important economic significance to the production of farm-forestry crop.Currently, the blueberry in production
Main breed, Ru Lanfeng, duke and blue beauty etc., has branch-rot, significantly affects its fruit yield and quality.
3. cercospora black spot of peanut bacterium: the cercospora black spot of peanut is commonly called as " black subcutaneous ulcer ", " black puckery ", is the domestic and international most common leaf in peanut producing region
Portion's fungal disease can all occur in the peanut entire season of growth, but its onset peak often has the middle and late growth stage in peanut, therefore
There is the title of " late spot " disease.It often results in plant largely to fall leaves, causes shell development to be obstructed, yield falls sharply.The germ only encroaches on peanut, so far
The present does not find other hosts still.Germ is mainly overwintering on invalid body with stroma, mycelia and conidium, can also be with ascocarp
In invalid body, or with conidium be attached to kind of a shell, on seed it is overwintering, become the source of morbidity in the 2nd year.When condition is suitable for
The conidium that germ mycelia directly generates is propagated with wind and rain.The scab of generation primarily occur ins in the old leaf of native table,
Nearly all leaf abscission can be caused before harvest.
4. tomato early blight bacterium: the disease may also suppress tomato growth and fruit formed in addition to directly endangering stem, leaf, fruit, dew
Ground and protecting field are aggrieved all heavier, long-term underproduction 20%-30%, up to 50% or more, or even total crop failure when serious.This cause of disease host
It is in extensive range, in addition to endangering tomato, it can also endanger the solanaceous vegetables crop such as eggplant, capsicum and potato.
5. botrytis cinerea: graw mold of tomato is that the pathogen of Botrytis cinerea infects caused Common Diseases, is that harm is maximum
One of disease occurs universal.In addition to endangering tomato, 20 various crop such as harmful eggplant, capsicum, cucumber, bottle gourd can be also endangered.It is low
Time harm more than warm, continuous rainy weather is serious.The withered and a large amount of etching of cauline leaf, decayed fruit are caused when falling ill serious, directly
Influence yield.The germ is a kind of facultative parasite that host range is very wide, can infect various fruits, vegetables and flowers.
6. fusarium graminearum: head blight is a kind of destructive disease, and fringe can be caused rotten, and Severe Reduction and quality is caused to drop
It is low.With the change of global warming, cropping system and mode, wheat scab constantly spreads extension, often results in wheat
The underproduction, quality reduce, and contain mycotoxin in the wheat seed infected, can cause person poultry poisoning and serious disease.The disease
Bacterium is in addition to endangering wheat, moreover it is possible to infect the grass family such as a variety of gramineous crops such as barley, oat, rice, corn and goose hat grass
Weeds, in addition, can also infect the crops such as soybean, cotton, sweet potato.
7. potato wilt bacterium: Fusarium oxysporum is the main pathogenic bacteria of potato wilt, and the bacterium is a kind of
Universal soil-borne disease fungal pathogens, host range is very extensive, can cause Solanaceae, melon, banana, pulse family, cotton and flowers
Etc. various plants wilt disease generation.After host is infected by Fusarium oxysporum, Symptoms are varied, generally result in vascular bundle
Brown stain, plant are wilted withered, bulb and root rot, and plant strain growth is weak etc..Potato wilt is to make in recent years to potato
At the important fungoid soil-borne disease of one kind of harm, have generation in many areas, journey especially occurs weakly in potato weight
It spends even more serious, directly affects potato yield and its economic benefit.
8. Tobacco target spot bacterium: Tobacco target spot is by the microbial tikka class disease of miliary damping-off, which mainly endangers
Evil blade forms scab, and frangible is formationed in scab necrosis part is perforated, when occurring serious scab in flakes, and plant disease epidemic speed compared with
Fastly, therefore tobacco leaf production is constituted and is seriously threatened.The host range of the germ is extensive, tobacco can be not only infected, in nature
In the case of can infect the cereal crops such as 200 various plants, including corn, sorghum, cotton, wheat, rice, soybean and beet, eggplant
Son, tomato, black nightshade, honor fiber crops and petunia etc..In addition, the germ can infect 188 kinds of plants of 32 sections under artificial inoculation conditions
Object.
9. verticillium dahliae: cause cotton verticillium wilt pathogen be Verticillium dahliae belong to verticillium dahliae.The pathogen
Host range it is extremely wide, foreign countries' report can cause harm 38 section, 660 kinds of plants, wherein 184 kinds of crops, 153 kinds of weeds.China is through reflecting
Fixed, host plant at least 80 kinds of 20 section, field crop includes cotton, sunflower, eggplant, spicy, tomato, tobacco, potato, sweet tea
Melon, watermelon, cucumber, peanut, Kidney bean, mung bean, soybean, sesame, beet etc..The germ can make host plant generate verticillium wilt,
It is resulted in significant economic losses in world wide.Verticillium wilt prevention and treatment is difficult, and such as biological control of existing means of prevention, chemistry are anti-
It controls, conventional genetic breeding etc. is all unable to reach ideal control efficiency.
10. Maize Curvularia: Maize Curvularia Leaf Spot Disease also known as quasi- eye spot, black points, mainly cause harm maize leaves
Piece also endangers leaf sheath and bract sometimes, and the variation of its symptom is very big in different cultivars.The germ host range is wider, can post
It is born on the fruit of tomato, capsicum, can also colonize on rice, sorghum, wheat and some lawns, so that these plants occur
Disease.Maize Curvularia Leaf Spot Disease is a kind of disease that China North China, northeast corn producing region generally occur in recent years, since the disease is
Sprawling occurs for sudden disease rapidly, and leaf portion scab is intensively in blocks when serious, and grave illness plot diseased plant rate and sick leaf rate are up to
100%, it has also become one of the important disease in China corn producing region.
11. cucumber stick spore leaf spot fungi: the germ host range is extensive, circulation way multiplicity.In recent years further investigation reveals that,
Shandong, Hebei, Liaoning, the Inner Mongol of the leaf spot of the vegetables such as cucumber, tomato as caused by cucumber stick spore leaf spot fungi in China
Occur Deng 11 province, city and region's large area, causes serious economic loss.
12. apple anthrax leaf spoting bacteria: apple anthrax leaf blight is a kind of leaf diseases as caused by anthrax bacteria, mainly
Endanger the kinds such as loud, high-pitched sound drawing, Jin Guan, Qiao Najin, Qin Guan of apple, cause apple tree largely fall leaves and fruit seriously rot, tree vigo(u)r is tight
Weaken again, next year is caused to be had no harvest.Fuji, red magnitude kind height are disease-resistant, and the loud, high-pitched sound of morbidity is drawn and the adjacent Fuji apple not fallen ill
Tree compares clearly in orchard.Apple anthrax-bacilus leaf blight main harm blade, and cause largely to fall leaves.
13. pears rotten pathogenic bacteria: pears rot disease also known as bark rot, the major branch and side shoot of main harm pear tree lead to pear tree gesture
It is weak, fruit yield and quality decline.The disease has the characteristics that generation area is wide, disease incidence is high, is difficult to control.In China pears master
There is generation in producing region, is occurred especially with Xinjiang, northwest, North China, northeast and other places serious.It falls ill serious theatre, tree body disease scar is innumerable,
Limb is incomplete, causes a large amount of gardens Si Shuhuohui.
14. Botryosphaeria berengeriana f. sp: ring rot of apple also known as scurf, wheel line decayed fruit disease, are on apple limb portion and fruit
Important biomolecule disaster, Chang Yinqi apple limb bark is coarse, locality is downright bad and fruit rot.Illness plant fruit-setting rate is low,
Cause tree body weak and yield is reduced, or even garden is ruined in total crop failure.In recent years, with the commerial growing of susceptible kind Fuji apple,
Ring rot of apple disease incidence increases year by year, and hazard area constantly expands, it has also become the serious plant disease in Chinese apple production, seriously
Threaten the sustainable development of Apple Industry.
15. blueberry limb ulcer bacteria: the pathogen-Botryosphaeria for causing blueberry limb canker is a kind of world
Property distribution fungi, caused Tree resistance is the great disease occurred in world wide.The host that it is endangered is very wide, reaches
45 belong to (broad leaf tree 40 belong to, 5, coniferous tree category), woods fruit tree is endangered it is serious, can cause eucalyptus, poplar, apple, pomegranate,
The symptoms such as the limb ulcer withered deaths of the host plants such as peach, Cassia occidentalis, red bayberry, Chinese chestnut, dry rot, gummosis.
16. Valsa mali: apple tree canker is that apple tree is main, trunk disease of most serious.The disease in addition to
Fruit can also be infected outside limb by infecting, and caused deadwood, dead tree, caused to ruin garden when serious, to cause serious yield and warp
Ji loss.Apple rot pathogen possesses the extensive host plant of comparison, endangers including apple, Lou Tao, peach and pears etc.
Common fruit tree.
C14H10Cl2NNaO2 is shown in Table 1 to the in vitro bacteriostasis testing result of 16 kinds of phytopathogens.
The inhibiting effect measurement result (5d) that table 1 grows 16 kinds of pathogen mycelia
Note: each processing is set in test repeats three times, and data are duplicate average value three times in table.
As can be known from Table 1, with the raising of Diclofenac na concn, also have to the inhibitory effect of 16 kinds of phytopathogens
Different degrees of raising.When Diclofenac na concn is 1.0mM, wherein intending stem point branch-rot bacterium, graw mold of tomato to blueberry
The inhibiting rate of bacterium, Tobacco target spot bacterium, pears rotten pathogenic bacteria, blueberry limb ulcer bacteria and Valsa mali etc. is 80%
More than.When Diclofenac na concn is 1.5mM, blueberry intends stem point branch-rot bacterium, cercospora black spot of peanut bacterium, Tobacco target spot bacterium etc.
The inhibiting rate of 9 kinds of phytopathogens is more than 90%, can be effectively prevented to it.
The fungistatic effect picture that C14H10Cl2NNaO2 grows 16 kinds of phytopathogen mycelia as illustrated in figs. 1-16, when double chlorine are fragrant
For sour sodium in 1.5mM, blueberry intends stem point branch-rot bacterium, cercospora black spot of peanut bacterium, Tobacco target spot bacterium, verticillium dahliae, cucumber
The bacterium of stick spore leaf spot fungi, apple anthrax leaf spoting bacteria, Botryosphaeria berengeriana f. sp, blueberry limb ulcer bacteria and Valsa mali
Silk is not grown substantially.The mycelia growth of other plant pathogen is weaker or less, illustrates that C14H10Cl2NNaO2 plays well
Inhibitory effect.In conclusion in the very potential prevention and treatment applied to phytopathogen of C14H10Cl2NNaO2.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than is limited;Although referring to aforementioned reality
Applying example, invention is explained in detail, for those of ordinary skill in the art, still can be to aforementioned implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features;And these are modified or replace
It changes, the spirit and scope for claimed technical solution of the invention that it does not separate the essence of the corresponding technical solution.
Claims (7)
1. C14H10Cl2NNaO2 is in preparation for preventing and treating the application in the fungicide by the microbial plant disease of pathogenic.
2. C14H10Cl2NNaO2 according to claim 1 is in preparation for preventing and treating by the microbial plant disease of pathogenic
Application in fungicide, it is characterised in that: the phytopathogen is Deuteromycotina fungi and Ascomycotina fungi.
3. C14H10Cl2NNaO2 according to claim 1 or 2 is in preparation for preventing and treating by the microbial phytopathy of pathogenic
Application in harmful fungicide, it is characterised in that: the effective of C14H10Cl2NNaO2 uses concentration for 0.5 mM-in the fungicide
1.5 mM。
4. C14H10Cl2NNaO2 according to claim 1 or 2 is in preparation for preventing and treating by the microbial phytopathy of pathogenic
Application in harmful fungicide, it is characterised in that: the Deuteromycotina fungi includes: that blueberry intends stem point branch-rot bacterium, blueberry
Branch-rot bacterium, cercospora black spot of peanut bacterium, tomato early blight bacterium, botrytis cinerea, fusarium graminearum, potato wilt bacterium,
Tobacco target spot bacterium, verticillium dahliae, Maize Curvularia and cucumber stick spore leaf spot fungi.
5. C14H10Cl2NNaO2 according to claim 1 or 2 is in preparation for preventing and treating by the microbial phytopathy of pathogenic
Application in harmful fungicide, it is characterised in that: the Ascomycotina fungi includes apple anthrax leaf spoting bacteria, pears rot disease
Bacterium, Botryosphaeria berengeriana f. sp, blueberry limb ulcer bacteria and Valsa mali.
6. C14H10Cl2NNaO2 according to claim 1 is in preparation for preventing and treating by the microbial plant disease of pathogenic
Application in fungicide, it is characterised in that: when the concentration of C14H10Cl2NNaO2 is 1.5mM in the fungicide, C14H10Cl2NNaO2 pair
Blueberry intends stem point branch-rot bacterium, cercospora black spot of peanut bacterium, Tobacco target spot bacterium, verticillium dahliae, cucumber stick spore leaf spot fungi, apple
Fruit anthrax leaf spoting bacteria, Botryosphaeria berengeriana f. sp, blueberry limb ulcer bacteria and Valsa mali inhibiting rate be more than 90%.
7. C14H10Cl2NNaO2 according to claim 1 is in preparation for preventing and treating by the microbial plant disease of pathogenic
Application in fungicide, it is characterised in that: the plant is apple, pears, tomato, blueberry, tobacco, cucumber, cotton, corn, small
Wheat, potato and peanut.
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CN116171996A (en) * | 2022-12-30 | 2023-05-30 | 华南农业大学 | Hypoxia-resistant seed soaking agent for flooded direct-seeding rice and application thereof |
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CN104920367A (en) * | 2015-06-18 | 2015-09-23 | 青岛农业大学 | Application of nicotinamide for preparing fungicide used for controlling plant diseases caused by pathogenic fungi |
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CN116171996A (en) * | 2022-12-30 | 2023-05-30 | 华南农业大学 | Hypoxia-resistant seed soaking agent for flooded direct-seeding rice and application thereof |
CN116171996B (en) * | 2022-12-30 | 2024-04-12 | 华南农业大学 | Hypoxia-resistant seed soaking agent for flooded direct-seeding rice and application thereof |
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