CN104520713A - 在单个样品上的免疫荧光和基于荧光的核酸分析 - Google Patents

在单个样品上的免疫荧光和基于荧光的核酸分析 Download PDF

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Publication number
CN104520713A
CN104520713A CN201380017702.4A CN201380017702A CN104520713A CN 104520713 A CN104520713 A CN 104520713A CN 201380017702 A CN201380017702 A CN 201380017702A CN 104520713 A CN104520713 A CN 104520713A
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China
Prior art keywords
image
sample
nucleic acid
fluorescent
binding agent
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Pending
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CN201380017702.4A
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English (en)
Chinese (zh)
Inventor
A.E.塞波
F.金蒂
K.B.肯尼
D.L.亨德森
M.J.格德斯
A.I.拉里拉莫雷诺
X.刘
A.D.科文
S.E.青格列维奇
T.哈
N.R.军
A.基什图巴耶夫
D.A.霍尔曼-休格利
Y.李
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Global Life Sciences Solutions USA LLC
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Clarient Diagnostic Services Inc
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Publication of CN104520713A publication Critical patent/CN104520713A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/5752—Immunoassay; Biospecific binding assay; Materials therefor for cancer of the lungs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57515—Immunoassay; Biospecific binding assay; Materials therefor for cancer of the breast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/71—Assays involving receptors, cell surface antigens or cell surface determinants for growth factors; for growth regulators

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201380017702.4A 2012-03-30 2013-03-21 在单个样品上的免疫荧光和基于荧光的核酸分析 Pending CN104520713A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261618132P 2012-03-30 2012-03-30
US61/618132 2012-03-30
PCT/US2013/033249 WO2013148448A1 (en) 2012-03-30 2013-03-21 Immunoflu0rescence and fluorescent-based nucleic acid analysis on a single sample

Publications (1)

Publication Number Publication Date
CN104520713A true CN104520713A (zh) 2015-04-15

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CN201380017702.4A Pending CN104520713A (zh) 2012-03-30 2013-03-21 在单个样品上的免疫荧光和基于荧光的核酸分析

Country Status (12)

Country Link
US (2) US20150065371A1 (https=)
EP (1) EP2831591B1 (https=)
JP (1) JP6225163B2 (https=)
KR (1) KR20140142715A (https=)
CN (1) CN104520713A (https=)
AU (1) AU2013240127B2 (https=)
CA (1) CA2867508C (https=)
IN (1) IN2014DN07531A (https=)
NZ (1) NZ630091A (https=)
RU (1) RU2014137129A (https=)
SG (1) SG11201406105UA (https=)
WO (1) WO2013148448A1 (https=)

Cited By (8)

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CN106290878A (zh) * 2016-09-10 2017-01-04 天津大学 基于量子点的胰腺癌肿瘤标志物ca242免疫层析试纸条的制备方法
CN107419001A (zh) * 2016-05-23 2017-12-01 益善生物技术股份有限公司 Brca1、pten基因异常检测试剂盒及检测方法
CN107847145A (zh) * 2015-07-20 2018-03-27 徐敏 光子结构和化学计量学病理系统
CN108572163A (zh) * 2016-05-31 2018-09-25 希森美康株式会社 荧光图像分析装置、分析方法及预处理的评价方法
CN109791157A (zh) * 2016-09-26 2019-05-21 赛卢拉研究公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
CN110498858A (zh) * 2019-07-26 2019-11-26 深圳市达科为生物工程有限公司 一种动态检测单细胞外泌蛋白分泌情况的方法
CN112415198A (zh) * 2020-11-20 2021-02-26 四川大学华西医院 Gp1bb检测试剂在制备肺癌筛查试剂盒中的用途
CN112840259A (zh) * 2018-10-12 2021-05-25 深圳市诺高实验器材有限公司 用于染色的过程记录载玻片及其使用方法

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US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
CN108267581A (zh) 2012-08-24 2018-07-10 耶鲁大学 用于高通量多重检测的系统、装置和方法
US10088658B2 (en) 2013-03-18 2018-10-02 General Electric Company Referencing in multi-acquisition slide imaging
JP6605506B2 (ja) * 2014-02-24 2019-11-13 ヴェンタナ メディカル システムズ， インク． ＣＤ３、ＣＤ８、ＣＤ２０及びＦｏｘＰ３の同時検出によりがんに対する免疫応答をスコア化するための方法、キット、及びシステム
CN104980558B (zh) * 2014-04-01 2019-01-01 中国移动通信集团公司 一种信息处理的方法、装置、手机宠物客户端及移动终端
WO2015157246A2 (en) * 2014-04-07 2015-10-15 Massachusetts Institute Of Technology Use of microparticle additives to simultaneously enable artifact-free image registration, auto-focusing, and chromatic aberration correction in microscopy
WO2016026038A1 (en) * 2014-08-18 2016-02-25 Viewsiq Inc. System and method for embedded images in large field-of-view microscopic scans
CN112782140A (zh) * 2014-12-03 2021-05-11 伊索普莱西斯公司 细胞分泌特征的分析和筛选
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
EP3396375B1 (en) * 2015-12-24 2021-08-04 Konica Minolta, Inc. Image processing device and program
EP3469548B1 (en) 2016-06-10 2020-05-13 H. Hoffnabb-La Roche Ag System for bright field image simulation
EP3510398B1 (en) 2016-09-12 2026-02-25 Isoplexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
EP4036578A1 (en) 2016-11-11 2022-08-03 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
CN110226084A (zh) 2016-11-22 2019-09-10 伊索普莱克西斯公司 用于细胞捕获的系统、装置和方法，以及其制造方法
CN112601950B (zh) * 2018-08-22 2023-07-28 仪景通株式会社 图像处理装置、摄像系统、图像处理装置的工作方法以及图像处理装置的工作程序
US20210182531A1 (en) * 2019-12-17 2021-06-17 Applied Materials, Inc. Registration techniques for multiplexed fluorescence in-situ hybridization images
EP4147044A4 (en) * 2020-05-07 2024-06-12 Advanced Cell Diagnostics, Inc. METHOD FOR THE SIMULTANEOUS DETECTION OF TARGET NUCLEIC ACIDS AND PROTEINS AND KIT THEREFOR
US12235215B2 (en) * 2020-07-20 2025-02-25 Akoya Biosciences, Inc. Processing and imaging tissue samples
WO2022035943A1 (en) * 2020-08-13 2022-02-17 Ventana Medical Systems, Inc. Automated identification of necrotic regions in digital images of multiplex immunofluorescence stained tissue
EP4491742B1 (en) * 2020-09-18 2026-03-04 10X Genomics, Inc. Sample handling apparatus and image registration methods
CN113310963B (zh) * 2021-05-31 2023-12-01 四川大学华西医院 一种改进的中性粒细胞NETs免疫荧光检测方法
US20250000906A1 (en) 2021-11-16 2025-01-02 Sotio Biotech Inc. Treatment of myxoid/round cell liposarcoma patients
US20250216394A1 (en) * 2022-01-28 2025-07-03 University Of Southern California Liquid biopsy analytes to define cancer stages
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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107847145A (zh) * 2015-07-20 2018-03-27 徐敏 光子结构和化学计量学病理系统
CN107419001A (zh) * 2016-05-23 2017-12-01 益善生物技术股份有限公司 Brca1、pten基因异常检测试剂盒及检测方法
CN108572163A (zh) * 2016-05-31 2018-09-25 希森美康株式会社 荧光图像分析装置、分析方法及预处理的评价方法
CN108572163B (zh) * 2016-05-31 2022-06-03 希森美康株式会社 荧光图像分析装置、分析方法及预处理的评价方法
CN106290878A (zh) * 2016-09-10 2017-01-04 天津大学 基于量子点的胰腺癌肿瘤标志物ca242免疫层析试纸条的制备方法
CN109791157A (zh) * 2016-09-26 2019-05-21 赛卢拉研究公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
CN109791157B (zh) * 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
CN112840259A (zh) * 2018-10-12 2021-05-25 深圳市诺高实验器材有限公司 用于染色的过程记录载玻片及其使用方法
CN110498858A (zh) * 2019-07-26 2019-11-26 深圳市达科为生物工程有限公司 一种动态检测单细胞外泌蛋白分泌情况的方法
CN110498858B (zh) * 2019-07-26 2024-01-23 深圳市达科为生物工程有限公司 一种动态检测单细胞外泌蛋白分泌情况的方法
CN112415198A (zh) * 2020-11-20 2021-02-26 四川大学华西医院 Gp1bb检测试剂在制备肺癌筛查试剂盒中的用途

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KR20140142715A (ko) 2014-12-12
WO2013148448A1 (en) 2013-10-03
RU2014137129A (ru) 2016-05-27
JP2015514212A (ja) 2015-05-18
IN2014DN07531A (https=) 2015-04-24
US20150065371A1 (en) 2015-03-05
NZ630091A (en) 2016-03-31
AU2013240127B2 (en) 2018-07-12
US20250102507A1 (en) 2025-03-27
AU2013240127A1 (en) 2014-10-02
SG11201406105UA (en) 2014-10-30
CA2867508A1 (en) 2013-10-03
EP2831591B1 (en) 2019-05-08
JP6225163B2 (ja) 2017-11-01
EP2831591A1 (en) 2015-02-04
CA2867508C (en) 2021-05-18

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