CA2867508C - Immunofluorescence and fluorescent-based nucleic acid analysis on a single sample - Google Patents

Immunofluorescence and fluorescent-based nucleic acid analysis on a single sample Download PDF

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Publication number
CA2867508C
CA2867508C CA2867508A CA2867508A CA2867508C CA 2867508 C CA2867508 C CA 2867508C CA 2867508 A CA2867508 A CA 2867508A CA 2867508 A CA2867508 A CA 2867508A CA 2867508 C CA2867508 C CA 2867508C
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Prior art keywords
nucleic acid
biological sample
composite image
immunofluorescence
fluorescent
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CA2867508A
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English (en)
French (fr)
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CA2867508A1 (en
Inventor
Antti E. SEPPO
Fiona Ginty
Kevin B. Kenny
David Lavan Henderson
Michael J. Gerdes
Adriana I. Larriera Moreno
Xiaofeng Liu
Alex D. Corwin
Stephen E. Zingelewicz
Thomas Ha
Natalie R. Jun
Ainura KYSHTOOBAYEVE
Denise A HOLLMAN-HEWGLEY
Ying Li
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Leica Microsystems CMS GmbH
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Global Life Sciences Solutions USA LLC
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Publication of CA2867508A1 publication Critical patent/CA2867508A1/en
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Publication of CA2867508C publication Critical patent/CA2867508C/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/5752—Immunoassay; Biospecific binding assay; Materials therefor for cancer of the lungs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57515—Immunoassay; Biospecific binding assay; Materials therefor for cancer of the breast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/71—Assays involving receptors, cell surface antigens or cell surface determinants for growth factors; for growth regulators

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
CA2867508A 2012-03-30 2013-03-21 Immunofluorescence and fluorescent-based nucleic acid analysis on a single sample Active CA2867508C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261618132P 2012-03-30 2012-03-30
US61/618,132 2012-03-30
PCT/US2013/033249 WO2013148448A1 (en) 2012-03-30 2013-03-21 Immunoflu0rescence and fluorescent-based nucleic acid analysis on a single sample

Publications (2)

Publication Number Publication Date
CA2867508A1 CA2867508A1 (en) 2013-10-03
CA2867508C true CA2867508C (en) 2021-05-18

Family

ID=48050953

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2867508A Active CA2867508C (en) 2012-03-30 2013-03-21 Immunofluorescence and fluorescent-based nucleic acid analysis on a single sample

Country Status (12)

Country Link
US (2) US20150065371A1 (https=)
EP (1) EP2831591B1 (https=)
JP (1) JP6225163B2 (https=)
KR (1) KR20140142715A (https=)
CN (1) CN104520713A (https=)
AU (1) AU2013240127B2 (https=)
CA (1) CA2867508C (https=)
IN (1) IN2014DN07531A (https=)
NZ (1) NZ630091A (https=)
RU (1) RU2014137129A (https=)
SG (1) SG11201406105UA (https=)
WO (1) WO2013148448A1 (https=)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
EP4148118A1 (en) 2012-08-24 2023-03-15 Yale University System, device and method for high-throughput multi-plexed detection
US10088658B2 (en) 2013-03-18 2018-10-02 General Electric Company Referencing in multi-acquisition slide imaging
CA2940115C (en) 2014-02-24 2023-03-14 Ventana Medical Systems, Inc. Methods, kits, and systems for scoring the immune response to cancer by simultaneous detection of cd3, cd8, cd20 and foxp3
CN104980558B (zh) * 2014-04-01 2019-01-01 中国移动通信集团公司 一种信息处理的方法、装置、手机宠物客户端及移动终端
WO2015157246A2 (en) * 2014-04-07 2015-10-15 Massachusetts Institute Of Technology Use of microparticle additives to simultaneously enable artifact-free image registration, auto-focusing, and chromatic aberration correction in microscopy
WO2016026038A1 (en) * 2014-08-18 2016-02-25 Viewsiq Inc. System and method for embedded images in large field-of-view microscopic scans
US10584366B2 (en) 2014-12-03 2020-03-10 IsoPlexis Corporation Analysis and screening of cell secretion profiles
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
CN107847145B (zh) * 2015-07-20 2020-08-14 徐敏 光子结构和化学计量学病理系统
EP3396375B1 (en) * 2015-12-24 2021-08-04 Konica Minolta, Inc. Image processing device and program
CN107419001A (zh) * 2016-05-23 2017-12-01 益善生物技术股份有限公司 Brca1、pten基因异常检测试剂盒及检测方法
JP6231709B1 (ja) * 2016-05-31 2017-11-15 シスメックス株式会社 蛍光画像分析装置および分析方法
WO2017212055A1 (en) * 2016-06-10 2017-12-14 F. Hoffmann-La Roche Ag System for bright field image simulation
CN106290878A (zh) * 2016-09-10 2017-01-04 天津大学 基于量子点的胰腺癌肿瘤标志物ca242免疫层析试纸条的制备方法
WO2018049418A1 (en) 2016-09-12 2018-03-15 IsoPlexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
CN109791157B (zh) * 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
EP3538891B1 (en) 2016-11-11 2022-01-05 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
US11525783B2 (en) 2016-11-22 2022-12-13 IsoPlexis Corporation Systems, devices and methods for cell capture and methods of manufacture thereof
WO2020039520A1 (ja) * 2018-08-22 2020-02-27 オリンパス株式会社 画像処理装置、撮像システム、画像処理装置の作動方法、及び画像処理装置の作動プログラム
US10921223B2 (en) * 2018-10-12 2021-02-16 Shenzhen Prs Limited Process record slide for staining and method of using the same
CN110498858B (zh) * 2019-07-26 2024-01-23 深圳市达科为生物工程有限公司 一种动态检测单细胞外泌蛋白分泌情况的方法
US20210182531A1 (en) * 2019-12-17 2021-06-17 Applied Materials, Inc. Registration techniques for multiplexed fluorescence in-situ hybridization images
EP4147044A4 (en) * 2020-05-07 2024-06-12 Advanced Cell Diagnostics, Inc. METHOD FOR THE SIMULTANEOUS DETECTION OF TARGET NUCLEIC ACIDS AND PROTEINS AND KIT THEREFOR
EP4182676A1 (en) * 2020-07-20 2023-05-24 Akoya Biosciences, Inc. Processing and imaging tissue samples
WO2022035943A1 (en) 2020-08-13 2022-02-17 Ventana Medical Systems, Inc. Automated identification of necrotic regions in digital images of multiplex immunofluorescence stained tissue
WO2022061150A2 (en) * 2020-09-18 2022-03-24 10X Geonomics, Inc. Sample handling apparatus and image registration methods
CN112415198B (zh) * 2020-11-20 2022-11-11 四川大学华西医院 Gp1bb检测试剂在制备肺癌筛查试剂盒中的用途
CN113310963B (zh) * 2021-05-31 2023-12-01 四川大学华西医院 一种改进的中性粒细胞NETs免疫荧光检测方法
AU2022388928A1 (en) 2021-11-16 2024-05-16 Sotio Biotech Inc. Treatment of myxoid/round cell liposarcoma patients
WO2023147133A1 (en) * 2022-01-28 2023-08-03 University Of Southern California Liquid biopsy analytes to define cancer stages
CA3243342A1 (en) * 2022-01-28 2023-08-03 University Of Southern California IDENTIFICATION OF NON-DISEALED PATIENTS USING DISEASE-ASSOCIATED ASSAY AND ANALYSIS IN LIQUID BIOPSY

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6404906B2 (en) 1997-03-03 2002-06-11 Bacus Research Laboratories,Inc. Method and apparatus for acquiring and reconstructing magnified specimen images from a computer-controlled microscope
US6993169B2 (en) * 2001-01-11 2006-01-31 Trestle Corporation System and method for finding regions of interest for microscopic digital montage imaging
US7629125B2 (en) * 2006-11-16 2009-12-08 General Electric Company Sequential analysis of biological samples
US20110189670A1 (en) * 2008-07-07 2011-08-04 Ruth L Katz Circulating Tumor and Tumor Stem Cell Detection Using Genomic Specific Probes
US8269827B2 (en) * 2009-09-29 2012-09-18 General Electric Company System and methods for mapping fluorescent images into a bright field color space

Also Published As

Publication number Publication date
JP6225163B2 (ja) 2017-11-01
IN2014DN07531A (https=) 2015-04-24
CN104520713A (zh) 2015-04-15
KR20140142715A (ko) 2014-12-12
AU2013240127B2 (en) 2018-07-12
US20150065371A1 (en) 2015-03-05
US20250102507A1 (en) 2025-03-27
NZ630091A (en) 2016-03-31
AU2013240127A1 (en) 2014-10-02
EP2831591A1 (en) 2015-02-04
EP2831591B1 (en) 2019-05-08
RU2014137129A (ru) 2016-05-27
SG11201406105UA (en) 2014-10-30
WO2013148448A1 (en) 2013-10-03
JP2015514212A (ja) 2015-05-18
CA2867508A1 (en) 2013-10-03

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