CN104488551A - Ganoderma tsugae strain cultivation method - Google Patents
Ganoderma tsugae strain cultivation method Download PDFInfo
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- CN104488551A CN104488551A CN201410789678.2A CN201410789678A CN104488551A CN 104488551 A CN104488551 A CN 104488551A CN 201410789678 A CN201410789678 A CN 201410789678A CN 104488551 A CN104488551 A CN 104488551A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention provides a ganoderma tsugae strain cultivation method and particularly relates to the technical field of cultivation and processing of fungi. The method includes the four steps of mother strain cultivation, stock culture production, inoculation cultivation and fruiting management. The cultivated ganoderma tsugae strain is high in content of active ingredient and good in anti-tumor function and has good economic and social benefits, and the cultivation method is economical, practical, low in cost and good in effect; due to substrates mainly composed of grain materials and addition of humic acid, poison to the strain due to heavy metal in soil is reduced; the technique of simulating the growth environment of the wild ganoderma tsugae is adopted, so that yield of the ganoderma tsugae is increased while quality of the same is improved, and safety of the ganoderma tsugae products is guaranteed. The method is beneficial to standardization, industrialization and internationalization of the traditional Chinese medicine ganoderma tsugae products; quality of the ganoderma tsugae is improved highly, the active ingredient content is increased significantly, and good economic and social benefits are gained.
Description
Technical field
The invention belongs to the cultivation of fungi and the technical field of processing, especially relate to a kind of cultivation method of Ganoderma tsugae bacterial classification.
Background technology
Ganoderma tsugae is embodied in China's Chinese medicine pharmacopeia, is widely used in the middle of antitumor medicine and health food together with red sesame, purple sesame.Through fluctuation-type development for many years, Ganoderma tsugae from unidirectional Export Development to multiple converted products inside and outside the situation of selling.And wild Ganoderma tsugae is subject to pursuing of people due to the growing environment of its uniqueness, the traditional concept of green safety, and price also remains high always.At present, about Ganoderma tsugae patent application mainly about Ganoderma tsugae extraction, polysaccharide preparation, fruiting body molecule mark or have the cultivation method of bacterial classification.Employing juggle, corncob, cotton seed hull, weeds are the Ganoderma tsugae that raw material is produced, and its quality is often uneven, and active ingredient is different., the property of medicine on the low side by pure cotton seed hulls plantation output declines, and produces glossy ganoderma safety instability with weeds, Chinese medicine slag as raw material.
Summary of the invention
The problem to be solved in the present invention is to provide a kind of cultivation method of Ganoderma tsugae bacterial classification, Ganoderma tsugae bacterial activity composition prepared by the method is high, anti-tumor function is good, there is good economic benefit and social benefit, improve the yield and quality of Ganoderma tsugae, for the sustainable development of Ganoderma tsugae industry provides new way.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
A cultivation method for Ganoderma tsugae bacterial classification, comprises the following steps:
(1) female kind is made: gather the bacterial classification Ganoderma tsugae that tissue is separated, bacterial classification is connected in the short linden hole of sterilization, be placed in 30 DEG C of incubator constant temperature light culture, until mycelia covers with short linden hole namely obtain the female kind of production, on wherein said short linden hole medium formula be broken wheat 20-30 part, wheat bran 2-5 part, agar 2-5 part, calcium carbonate 0.3-0.7 part, calcium lactate 0.4-0.6 part, magnesium sulfate 0.15-0.25 part, vitamin 0.01-0.03 part, humus 0.10-0.15 part by mass percentage, water content 55-60%;
(2) original seed is made: will produce in the aseptic access original seed bag of female kind, be placed in 25 DEG C of incubator constant temperature light culture, namely original seed is obtained after mycelia eats full material, wherein the culture medium raw material composition of original seed bag is wheat bran 70-75 part, Chinese sorghum chaff 15-20 part, calcium carbonate 2-4 part, Radix Astragali extractive solution 0.01-0.03 part, angelica extract 0.01-0.04 part by mass percentage, water content 55-60%;
(3) inoculation cultivation: by aseptic for original seed access cultivation material bag, in 25 DEG C of culturing room, lucifuge is cultivated, after the mycelia in cultivation bag covers with planting material, continue shading After-mature cultivation and complete latter stage of ripening to mycelia, the high-pressure pulse direct current electric discharge that a secondary frequencies is 15MHz is carried out every one day, nature lightning in simulating forest environment, promote the growth of bacterial classification, wherein the culture medium raw material composition of planting material is wood chip 68-72 part, Chinese sorghum chaff 20-24 part, calcium carbonate 2-5 part by mass percentage, humus 0.1-0.3 part, water content 60-65%;
(4) sesame management is gone out: regulate the illumination 100-200lux that temperature is 25 DEG C, humidity 85-90%, flashing rate are 10HZ, stuffiness, keep CO
2content is at more than 2000ppm, and when stem to terminate and enter cap vegetative period elongating stage, ventilation, adjustment temperature is 26-28 DEG C, humidity 85-95%, and flashing rate is the illumination 400-500lux of 100HZ, ventilation, maintenance CO
2content is less than 1200ppm, when the white edge to cap turns yellow completely, plucks spore, thus obtains spore, new fresh sporophore, can obtain dry fruit body and Ganoderma tsugae mycelium after drying.
Radix Astragali extractive solution, angelica extract are utilize to pulverize the later Radix Astragali, Radix Angelicae Sinensis in described step (2), and becoming containing crude drug components by weight percent through high temperature, high pressure extraction is the extracting liq of 10%.
The relative air humidity of the culturing room in described step (3) is 60-70%.
The present invention prepares that Ganoderma tsugae bacterial activity composition is high, and anti-tumor function is good, has good economic benefit and social benefit, and the present invention is a kind of economical and practical, cultural method that cost is low, effective; Based on the composts or fertilisers of cultivating of grain class raw material, add humus and reduce the heavy metal of soil to the murder by poisoning of bacterial classification, adopt the growing environment technology of the wild Ganoderma tsugae of simulation, improve the yield and quality of Ganoderma tsugae, fundamentally solve Ganoderma tsugae produce and Forestry Ecological balance between contradiction, be also that the sustainable development of Ganoderma tsugae industry provides new way; Ganoderma tsugae Product Safety there is guarantee.Be conducive to traditional Chinese medicine Ganoderma tsugae product and move towards standardization, industrialization, international road; Make Ganoderma tsugae quality, active constituent content are significantly improved, obtain good economic benefit and social benefit.
Embodiment
Embodiment 1
A cultivation method for Ganoderma tsugae bacterial classification, comprises the following steps:
(1) female kind is made: gather the bacterial classification Ganoderma tsugae that tissue is separated, bacterial classification is connected in the short linden hole of sterilization, be placed in 30 DEG C of incubator constant temperature light culture, until mycelia covers with short linden hole namely obtain the female kind of production, on wherein said short linden hole medium formula be: broken wheat 20kg, wheat bran 5kg, agar 2kg, calcium carbonate 0.3kg, calcium lactate 0.4kg, magnesium sulfate 0.15kg, vitamin 0.01kg, humus 0.10kg, all the other are water, water content 55-60%;
(2) original seed is made: will produce in the aseptic access original seed bag of female kind, be placed in 25 DEG C of incubator constant temperature light culture, namely original seed is obtained after mycelia eats full material, Radix Astragali extractive solution 0.001kg, components by weight percent that wherein the culture medium raw material composition of original seed bag is 10% for wheat bran 70kg, Chinese sorghum chaff 20kg, calcium carbonate 2kg, components by weight percent are by mass percentage 10% angelica extract 0.001kg, water content 55-60%; Wherein, Radix Astragali extractive solution, angelica extract are utilize to pulverize the later Radix Astragali, Radix Angelicae Sinensis, and becoming containing crude drug components by weight percent through high temperature, high pressure extraction is the extracting liq of 10%.
(3) inoculation cultivation: by aseptic for original seed access cultivation material bag, in 25 DEG C of culturing room, lucifuge is cultivated, after the mycelia in cultivation bag covers with planting material, continue shading After-mature cultivation and complete latter stage of ripening to mycelia, the high-pressure pulse direct current electric discharge that a secondary frequencies is 15MHz is carried out every one day, nature lightning in simulating forest environment, promote the growth of bacterial classification, wherein the culture medium raw material composition of planting material is wood chip 68kg by mass percentage, Chinese sorghum chaff 24kg, calcium carbonate 2kg, humus 0.1kg, water content 60%, the relative air humidity of culturing room controls as 60-70%,
(4) sesame management is gone out: regulate the illumination 100-200lux that temperature is 25 DEG C, humidity 85-90%, flashing rate are 10HZ, stuffiness, keep CO
2content is at more than 2000ppm, and when stem to terminate and enter cap vegetative period elongating stage, ventilation, adjustment temperature is 26-28 DEG C, humidity 85-95%, and flashing rate is the illumination 400-500lux of 100HZ, ventilation, maintenance CO
2content is less than 1200ppm, when white edge to cap turns yellow completely, pluck spore, extract at stem 0.5-1cm place, ten million can not pull out together with mycoderma, spreads in order to avoid introduce insect pest disease, drier is put into after obtaining spore, new fresh sporophore, water content reaches 10%, and can obtain fruit body gross weight is 9.8kg, obtains Ganoderma tsugae mycelium 57kg.
Embodiment 2
A cultivation method for Ganoderma tsugae bacterial classification, comprises the following steps:
(1) female kind is made: gather the bacterial classification Ganoderma tsugae that tissue is separated, bacterial classification is connected in the short linden hole of sterilization, be placed in 30 DEG C of incubator constant temperature light culture, until mycelia covers with short linden hole namely obtain the female kind of production, on wherein said short linden hole medium formula be broken wheat 25kg, wheat bran 3kg, agar 4kg, calcium carbonate 0.5kg, calcium lactate 0.5kg, magnesium sulfate 0.20kg, vitamin 0.02kg, humus 0.13kg, water content 55-60% by mass percentage;
(2) original seed is made: will produce in the aseptic access original seed bag of female kind, be placed in 25 DEG C of incubator constant temperature light culture, namely original seed is obtained after mycelia eats full material, wherein the culture medium raw material composition of original seed bag is the angelica extract 0.003kg that wheat bran 72kg, Chinese sorghum chaff 17kg, calcium carbonate 3kg, the components by weight percent Radix Astragali extractive solution 0.002kg that is 10%, components by weight percent are 10%, water content 55-60% by mass percentage;
(3) inoculation cultivation: by aseptic for original seed access cultivation material bag, in 25 DEG C of culturing room, lucifuge is cultivated, after the mycelia in cultivation bag covers with planting material, continue shading After-mature cultivation and complete latter stage of ripening to mycelia, the high-pressure pulse direct current electric discharge that a secondary frequencies is 15MHz is carried out every one day, nature lightning in simulating forest environment, promote the growth of bacterial classification, wherein the culture medium raw material composition of planting material is wood chip 70kg by mass percentage, Chinese sorghum chaff 23kg, calcium carbonate 3kg, humus 0.2kg, water content 60-65%, the relative air humidity of culturing room controls as 60-70%,
(4) sesame management is gone out: regulate the illumination 100-200lux that temperature is 25 DEG C, humidity 85-90%, flashing rate are 10HZ, stuffiness, keep CO
2content is at more than 2000ppm, and when stem to terminate and enter cap vegetative period elongating stage, ventilation, adjustment temperature is 26-28 DEG C, humidity 85-95%, and flashing rate is the illumination 400-500lux of 100HZ, ventilation, maintenance CO
2content is less than 1200ppm, when white edge to cap turns yellow completely, pluck spore, extract at stem 0.5-1cm place, ten million can not pull out together with mycoderma, spreads in order to avoid introduce insect pest disease, drier is put into after obtaining spore, new fresh sporophore, water content reaches 10%, and can obtain fruit body gross weight is 9.5kg, obtains Ganoderma tsugae mycelium 55kg.
Embodiment 3
A cultivation method for Ganoderma tsugae bacterial classification, comprises the following steps:
(1) female kind is made: gather the bacterial classification Ganoderma tsugae that tissue is separated, bacterial classification is connected in the short linden hole of sterilization, be placed in 30 DEG C of incubator constant temperature light culture, until mycelia covers with short linden hole namely obtain the female kind of production, on wherein said short linden hole medium formula be broken wheat 30kg, wheat bran 5kg, agar 5kg, calcium carbonate 0.7kg, calcium lactate 0.6kg, magnesium sulfate 0.25kg, vitamin 0.03kg, humus 0.15kg, water content 55-60% by mass percentage;
(2) original seed is made: will produce in the aseptic access original seed bag of female kind, be placed in 25 DEG C of incubator constant temperature light culture, namely original seed is obtained after mycelia eats full material, wherein the culture medium raw material composition of original seed bag is the angelica extract 0.04kg that wheat bran 75kg, Chinese sorghum chaff 20kg, calcium carbonate 4kg, Radix Astragali extractive solution 0.03kg, components by weight percent are 10%, water content 55-60kg by mass percentage;
(3) inoculation cultivation: by aseptic for original seed access cultivation material bag, in 25 DEG C of culturing room, lucifuge is cultivated, after the mycelia in cultivation bag covers with planting material, continue shading After-mature cultivation and complete latter stage of ripening to mycelia, the high-pressure pulse direct current electric discharge that a secondary frequencies is 15MHz is carried out every one day, nature lightning in simulating forest environment, promote the growth of bacterial classification, wherein the culture medium raw material composition of planting material is wood chip 72kg by mass percentage, Chinese sorghum chaff 22kg, calcium carbonate 5kg, humus 0.3kg, water content 60-65%, the relative air humidity of culturing room controls as 60-70%,
(4) sesame management is gone out: regulate the illumination 100-200lux that temperature is 25 DEG C, humidity 85-90%, flashing rate are 10HZ, stuffiness, keep CO
2content is at more than 2000ppm, and when stem to terminate and enter cap vegetative period elongating stage, ventilation, adjustment temperature is 26-28 DEG C, humidity 85-95%, and flashing rate is the illumination 400-500lux of 100HZ, ventilation, maintenance CO
2content is less than 1200ppm, when white edge to cap turns yellow completely, pluck spore, extract at stem 0.5-1cm place, ten million can not pull out together with mycoderma, spreads in order to avoid introduce insect pest disease, drier is put into after obtaining spore, new fresh sporophore, water content reaches 10%, and can obtain fruit body gross weight is 9.7kg, obtains Ganoderma tsugae mycelium 60kg.
Above three embodiments of the present invention have been described in detail, but described content being only preferred embodiment of the present invention, can not being considered to for limiting practical range of the present invention.All equalizations done according to the present patent application scope change and improve, and all should still belong within patent covering scope of the present invention.
Claims (3)
1. a cultivation method for Ganoderma tsugae bacterial classification, is characterized in that: comprise the following steps:
(1) female kind is made: gather the bacterial classification Ganoderma tsugae that tissue is separated, bacterial classification is connected in the short linden hole of sterilization, be placed in 30 DEG C of incubator constant temperature light culture, until mycelia covers with short linden hole namely obtain the female kind of production, on wherein said short linden hole medium formula be broken wheat 20-30 part, wheat bran 2-5 part, agar 2-5 part, calcium carbonate 0.3-0.7 part, calcium lactate 0.4-0.6 part, magnesium sulfate 0.15-0.25 part, vitamin 0.01-0.03 part, humus 0.10-0.15 part by mass percentage, water content 55-60%;
(2) original seed is made: will produce in the aseptic access original seed bag of female kind, be placed in 25 DEG C of incubator constant temperature light culture, namely original seed is obtained after mycelia eats full material, wherein the culture medium raw material composition of original seed bag is wheat bran 70-75 part, Chinese sorghum chaff 15-20 part, calcium carbonate 2-4 part, Radix Astragali extractive solution 0.01-0.03 part, angelica extract 0.01-0.04 part by mass percentage, water content 55-60%;
(3) inoculation cultivation: by aseptic for original seed access cultivation material bag, in 25 DEG C of culturing room, lucifuge is cultivated, after the mycelia in cultivation bag covers with planting material, continue shading After-mature cultivation and complete latter stage of ripening to mycelia, the high-pressure pulse direct current electric discharge that a secondary frequencies is 15MHz is carried out every one day, nature lightning in simulating forest environment, promote the growth of bacterial classification, wherein the culture medium raw material composition of planting material is wood chip 68-72 part, Chinese sorghum chaff 20-24 part, calcium carbonate 2-5 part by mass percentage, humus 0.1-0.3 part, water content 60-65%;
(4) sesame management is gone out: regulate the illumination 100-200lux that temperature is 25 DEG C, humidity 85-90%, flashing rate are 10HZ, stuffiness, keep CO
2content is at more than 2000ppm, and when stem to terminate and enter cap vegetative period elongating stage, ventilation, adjustment temperature is 26-28 DEG C, humidity 85-95%, and flashing rate is the illumination 400-500lux of 100HZ, ventilation, maintenance CO
2content is less than 1200ppm, when the white edge to cap turns yellow completely, plucks spore, thus obtains spore, new fresh sporophore, can obtain dry fruit body and medicinal Ganoderma tsugae mycelium after drying.
2. the cultivation method of a kind of Ganoderma tsugae bacterial classification according to claim 1, it is characterized in that: Radix Astragali extractive solution, angelica extract are utilize to pulverize the later Radix Astragali, Radix Angelicae Sinensis in described step (2), becoming containing crude drug components by weight percent through high temperature, high pressure extraction is the extracting liq of 10%.
3. the cultivation method of a kind of Ganoderma tsugae bacterial classification according to claim 1, is characterized in that: the relative air humidity of the culturing room in described step (3) is 60-70%.
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104737819A (en) * | 2015-04-14 | 2015-07-01 | 马凤 | Method for cultivating ganoderma tsugae by taking off a half of bag |
| CN104798599A (en) * | 2015-04-22 | 2015-07-29 | 吉林农业大学 | Method for planting ganoderma tsugae by imitating wild growth in forest land |
| CN104956913A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Method for cultivating artificial natural ganoderma lucidum under high-altitude forest |
| CN105367316A (en) * | 2015-12-24 | 2016-03-02 | 桂林大野领御生物科技有限公司 | Lucid ganoderma culture medium |
| CN105367303A (en) * | 2015-12-24 | 2016-03-02 | 桂林大野领御生物科技有限公司 | Culture medium for culturing ganoderma lucidum |
| CN105384549A (en) * | 2015-12-24 | 2016-03-09 | 桂林大野领御生物科技有限公司 | Additive special for lucid ganoderma culture medium |
| CN106489542A (en) * | 2016-12-21 | 2017-03-15 | 黑龙江省农业科学院牡丹江分院 | A kind of Ganoderma tsugae quick-growing cultivation method |
| CN108307930A (en) * | 2018-03-27 | 2018-07-24 | 贵港市万升木业有限公司 | A kind of cultivation of glossy ganoderma matrix and preparation method thereof containing cedar sawdust |
| CN108440051A (en) * | 2018-03-27 | 2018-08-24 | 贵港市万升木业有限公司 | A kind of cultivation of glossy ganoderma matrix and preparation method thereof containing camphorwood dust |
| CN108718913A (en) * | 2018-03-27 | 2018-11-02 | 贵港市万升木业有限公司 | A kind of cultivation of glossy ganoderma matrix of cherry apple sawdust and preparation method thereof |
| CN109168974A (en) * | 2018-10-09 | 2019-01-11 | 湖北悟芝堂生物科技有限公司 | A kind of Wildmimic cultivation method of ganoderma lucidum |
| CN113575277A (en) * | 2021-08-11 | 2021-11-02 | 江苏华绿生物科技股份有限公司 | Sorghum bran culture medium and application thereof in industrial cultivation of white flammulina velutipes |
| CN115530005A (en) * | 2022-10-14 | 2022-12-30 | 韶关市五马寨菌业有限公司 | Method for tissue culture and rapid propagation of sweet ganoderma lucidum |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104737819A (en) * | 2015-04-14 | 2015-07-01 | 马凤 | Method for cultivating ganoderma tsugae by taking off a half of bag |
| CN104798599A (en) * | 2015-04-22 | 2015-07-29 | 吉林农业大学 | Method for planting ganoderma tsugae by imitating wild growth in forest land |
| CN104956913A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Method for cultivating artificial natural ganoderma lucidum under high-altitude forest |
| CN105367316A (en) * | 2015-12-24 | 2016-03-02 | 桂林大野领御生物科技有限公司 | Lucid ganoderma culture medium |
| CN105367303A (en) * | 2015-12-24 | 2016-03-02 | 桂林大野领御生物科技有限公司 | Culture medium for culturing ganoderma lucidum |
| CN105384549A (en) * | 2015-12-24 | 2016-03-09 | 桂林大野领御生物科技有限公司 | Additive special for lucid ganoderma culture medium |
| CN106489542A (en) * | 2016-12-21 | 2017-03-15 | 黑龙江省农业科学院牡丹江分院 | A kind of Ganoderma tsugae quick-growing cultivation method |
| CN108307930A (en) * | 2018-03-27 | 2018-07-24 | 贵港市万升木业有限公司 | A kind of cultivation of glossy ganoderma matrix and preparation method thereof containing cedar sawdust |
| CN108440051A (en) * | 2018-03-27 | 2018-08-24 | 贵港市万升木业有限公司 | A kind of cultivation of glossy ganoderma matrix and preparation method thereof containing camphorwood dust |
| CN108718913A (en) * | 2018-03-27 | 2018-11-02 | 贵港市万升木业有限公司 | A kind of cultivation of glossy ganoderma matrix of cherry apple sawdust and preparation method thereof |
| CN109168974A (en) * | 2018-10-09 | 2019-01-11 | 湖北悟芝堂生物科技有限公司 | A kind of Wildmimic cultivation method of ganoderma lucidum |
| CN113575277A (en) * | 2021-08-11 | 2021-11-02 | 江苏华绿生物科技股份有限公司 | Sorghum bran culture medium and application thereof in industrial cultivation of white flammulina velutipes |
| CN115530005A (en) * | 2022-10-14 | 2022-12-30 | 韶关市五马寨菌业有限公司 | Method for tissue culture and rapid propagation of sweet ganoderma lucidum |
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