CN105384549A - Additive special for lucid ganoderma culture medium - Google Patents

Additive special for lucid ganoderma culture medium Download PDF

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Publication number
CN105384549A
CN105384549A CN201510982318.9A CN201510982318A CN105384549A CN 105384549 A CN105384549 A CN 105384549A CN 201510982318 A CN201510982318 A CN 201510982318A CN 105384549 A CN105384549 A CN 105384549A
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parts
culture medium
lucid ganoderma
radix
glossy ganoderma
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CN201510982318.9A
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蒋晓艳
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Guilin Daye Lingyu Biological Technology Co Ltd
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Guilin Daye Lingyu Biological Technology Co Ltd
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Priority to CN201510982318.9A priority Critical patent/CN105384549A/en
Publication of CN105384549A publication Critical patent/CN105384549A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention relates to the technical field of edible fungus culture medium addition, in particular to an additive special for a lucid ganoderma culture medium. The additive is prepared from, by weight, 20-40 parts of fructus cnidii, 10-20 parts of pericarpium granati, 5-10 parts of red halloysite, 10-20 parts of glutinous rice roots, 5-10 parts of blighted wheat, 5-10 parts of flatstem milkvetch seeds, 30-50 parts of radix ephedrae, 10-20 parts of radix acanthopanacis semticosi, 5-11 parts of Japanese ardisia herb, 10-30 parts of storax and 6-16 parts of white mulberry root-bark. When applied to the lucid ganoderma culture medium, the additive special for the lucid ganoderma culture medium promotes lucid ganoderma growth, increases the yield and meanwhile can improve the antineoplastic effect of lucid ganoderma.

Description

Medium for Ganoderma lucidum specialist additive
Technical field
The present invention relates to edible fungi culture medium additive technical field, particularly Medium for Ganoderma lucidum specialist additive.
Background technology
Glossy ganoderma contains multiple amino acids, protein, alkaloid, coumarin, steroid class, triterpenes, volatile oil, N.F,USP MANNITOL, resin and carbohydrate, Lin Suanna Vitamin B2 Sodium Phosphate, vitamins C, lactone and enzyme; Glossy ganoderma can tranquilizing by nourishing the heart, and quiet lung benefit gas, nourishing liver invigorating the spleen, cures mainly consumptive disease weak, spiritlessness and weakness, palpitation and insomnia, and the head swoons, and chronic cough is panted, and food is few indigestion and loss of appetite.Glossy ganoderma contains profuse rare elements " germanium ", the ability that blood of human body can be made to absorb oxygen increases by 1.5 times, therefore can enhance metabolism and have the effect of delaying retrogradation, also have the effect strengthening skin maintenance function own, the yellow complexion that can be used for caused by various chronic disease withers and the disease such as the facial radiance that causes due to insufficiency of vital energy and blood.Polysaccharide in glossy ganoderma has two-ways regulation immunity of organisms, the antitumor effect with protecting liver; Organic germanium contained by glossy ganoderma can be induced human body to produce and be activated NK cell and macrophage activity, participates in immunomodulatory; Alkaloid in glossy ganoderma can relieving inflammation and relaxing pain; Glossy ganoderma can regulating the flow of vital energy become silted up, pacify gas benefit god; Ganoderma spore powder can stop blooding, toxin expelling, antioxygen.
So for the medicinal glossy ganoderma having extremely strong effect, social demand is increasing, in order to the demand of satisfied society, start large batch of plantation glossy ganoderma, those skilled in the art are also studied the factor affecting glossy ganoderma quality and output, find that the impact of different planted medium on glossy ganoderma quality and output is very large.
Summary of the invention
Technical problem to be solved by this invention is to provide Medium for Ganoderma lucidum specialist additive, can also improve the antitumor efficacy of glossy ganoderma while promoting glossy ganoderma growth.
For achieving the above object, the present invention adopts following technical scheme:
Medium for Ganoderma lucidum specialist additive, be made up of the raw material of following weight part: Fructus Cnidii 20 ~ 40 parts, pomegranate rind 10 ~ 20 parts, Red Halloysite 5 ~ 10 parts, Root of Glutinous Rice 10 ~ 20 parts, shriveled wheat 5 ~ 10 parts, Semen Astragali Complanati 5 ~ 10 parts, Radix Ephedrae 30 ~ 50 parts, Radix Et Caulis Acanthopanacis Senticosi 10 ~ 20 parts, Japanese Ardisia Herb 5 ~ 11 parts, Styrax 10 ~ 30 parts, White Mulberry Root-bark 6 ~ 16 parts.
In raw material of the present invention:
Fructus Cnidii is the dry mature fruit of samphire cnidium monnieri Cnidiummonnieri (L.) Cuss..Warm in nature, taste is pungent, bitter.Warm in nature, taste is pungent, bitter.Warming the kidney to invigorate YANG, eliminating dampness, dispels the wind, desinsection.For under impotence, Gong Leng, cold-dampness band, arthritis with fixed pain caused by dampness pain in the back; Control vulval eczema, married woman's pruritus vulvae, trichomonal vaginitis outward.
Pomegranate rind is the dry pericarp of Punicaceae plant pomegranate PunicagranatumL..Sour, puckery, temperature.Relieving diarrhea with astringents, hemostasis, expelling parasite.For rushing down for a long time, protracted dysentery, has blood in stool, prolapse of the anus, uterine bleeding, leukorrhea, abdominal pain due to worm stagnation.
Red Halloysite, the red blocks of the one for silicates mineral halloysite.Sweet puckery, temperature.Puckery intestines, hemostasis, hygroscopic, myogenic.Control and rush down for a long time, protracted dysentery, have blood in stool, prolapse of the anus, seminal emission, uterine bleeding, under band, ulcer being unable to heal.
Root of Glutinous Rice is rhizome and the root of grass rice (glutinous rice).Sweet, flat.Reinforcing stomach reg fluid, reducing the asthenic fever, only night sweat.
Shriveled wheat is the shrivelled frivolous dry caryopsis of grass wheat TriticumaestivumL..Cool in nature, taste is sweet.Except abnormal heat, hidroschesis.Belong to the deficient heat clearing drug of antipyretic subordinate classification, or belong to the exterior-strengthening anhidrotic medicinal of astringent medicinal subordinate classification.
Semen Astragali Complanati is the dry mature seed of leguminous plants astragatus complanatus AstragaluscomplanatusR.Brown..Sweet, temperature.Temperature invigorating the liver and kidney, controlling nocturnal emission with astringent drugs, contracting urine, improving eyesight.For lumbago due to renal deficiency, seminal emission premature ejaculation, under gonorrhoea band, dribbling urination, dizzy blurred vision.
Radix Ephedrae is the dry root and rhizome of Ephedraceae plant plait Chinese ephedra EphedrasinicaStapf or epheday intermedia EphedraintermediaSchrenketC.A.Mey..Sweet, flat.Hidroschesis.For spontaneous perspiration, night sweat.
Radix Et Caulis Acanthopanacis Senticosi is the root and rhizome of stems and leaves of acanthopanax senticosus (Rupr.etMaxim.) Harms.Warm in nature, pungent, the micro-hardship of taste.Replenish qi to invigorate the spleen, tonify the kidney to relieve mental strain., poor appetite, soreness of waist and knee joint, insomnia and dreamful sleep weak for deficiency of spleen-YANG and kidneyYANG, body void.
Japanese Ardisia Herb is the complete stool of Myrsinacea plant Japanese Ardisia Herb Ardisiajaponica (Hornst.) B1..Property is put down, pungent, the micro-hardship of taste.Preventing phlegm from forming and stopping coughing, dampness removing, invigorates blood circulation.For cough, sputum mixed with blood, chronic bronchitis, jaundice due to damp-heat, injury from falling down.
Styrax, the resinoid that the trunk for Hamamelidaceae Liquidambar plant Styrax tree LiquidambarorientalisMill. oozes out, forms through processing and refining.
White Mulberry Root-bark is the dry root skin of moraceae plants mulberry MorusalbaL..Sweet, cold.Eliminating pathogen from the lung for relieving asthma, inducing diuresis to remove edema.For dyspnea and cough due to lung-heat, oedema turgor oliguria, appearance skin edema.
Preferably, Medium for Ganoderma lucidum specialist additive of the present invention, is made up of the raw material of following weight part: Fructus Cnidii 30 parts, pomegranate rind 15 parts, Red Halloysite 8 parts, Root of Glutinous Rice 15 parts, shriveled wheat 7 parts, Semen Astragali Complanati 8 parts, Radix Ephedrae 40 parts, Radix Et Caulis Acanthopanacis Senticosi 15 parts, Japanese Ardisia Herb 8 parts, Styrax 20 parts, White Mulberry Root-bark 11 parts.
Additive of the present invention addition in the substratum application of glossy ganoderma is 3 ~ 8% of substratum total amount.
Beneficial effect of the present invention is:
Containing the abundant trace element such as calcium, phosphorus, magnesium, sodium in the raw material of 1, Medium for Ganoderma lucidum specialist additive of the present invention, as the nutritive ingredient of glossy ganoderma, without the need to specially adding trace element materials, can reduce costs.
2, additive of the present invention is also containing biologically active substances such as alkaloid, polysaccharide, terpene, saponinses, and it has antibacterial and effect that is sterilization.
3, additive raw material compatibility of the present invention is scientific and reasonable, synergy, each other to correct its bias, eliminate or reduce toxicity, play the comprehensive action of to complement each other between each medicine or opposing each other and yet also complement each other, can promote that glossy ganoderma fully absorbs nutritive ingredient, improve the utilization ratio of medium nutrient content, promote the growth of glossy ganoderma, improve output.
4, additive of the present invention can strengthen the antitumor efficacy of glossy ganoderma.
5, additive of the present invention can replace part of main material, has widened culturing raw material channel, and Be very effective, greatly reduce production cost.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but the present invention is not limited to these embodiments.
Embodiment 1
Raw material is taken: Fructus Cnidii 20 parts, pomegranate rind 10 parts, Red Halloysite 5 parts, Root of Glutinous Rice 20 parts, shriveled wheat 10 parts, Semen Astragali Complanati 5 parts, Radix Ephedrae 50 parts, Radix Et Caulis Acanthopanacis Senticosi 10 parts, Japanese Ardisia Herb 11 parts, Styrax 30 parts, White Mulberry Root-bark 6 parts by following weight part; Mix, be crushed to 20 orders.
Embodiment 2
Raw material is taken: Fructus Cnidii 25 parts, pomegranate rind 13 parts, Red Halloysite 7 parts, Root of Glutinous Rice 18 parts, shriveled wheat 8 parts, Semen Astragali Complanati 7 parts, Radix Ephedrae 45 parts, Radix Et Caulis Acanthopanacis Senticosi 13 parts, Japanese Ardisia Herb 9 parts, Styrax 25 parts, White Mulberry Root-bark 8 parts by following weight part; Mix, be crushed to 40 orders.
Embodiment 3
Raw material is taken: Fructus Cnidii 30 parts, pomegranate rind 15 parts, Red Halloysite 8 parts, Root of Glutinous Rice 15 parts, shriveled wheat 7 parts, Semen Astragali Complanati 8 parts, Radix Ephedrae 40 parts, Radix Et Caulis Acanthopanacis Senticosi 15 parts, Japanese Ardisia Herb 8 parts, Styrax 20 parts, White Mulberry Root-bark 11 parts by following weight part; Mix, be crushed to 30 orders.
Embodiment 4
Raw material is taken: Fructus Cnidii 35 parts, pomegranate rind 18 parts, Red Halloysite 9 parts, Root of Glutinous Rice 13 parts, shriveled wheat 6 parts, Semen Astragali Complanati 9 parts, Radix Ephedrae 35 parts, Radix Et Caulis Acanthopanacis Senticosi 18 parts, Japanese Ardisia Herb 7 parts, Styrax 15 parts, White Mulberry Root-bark 13 parts by following weight part; Mix, be crushed to 50 orders.
Embodiment 5
Raw material is taken: Fructus Cnidii 40 parts, pomegranate rind 20 parts, Red Halloysite 10 parts, Root of Glutinous Rice 10 parts, shriveled wheat 5 parts, Semen Astragali Complanati 10 parts, Radix Ephedrae 30 parts, Radix Et Caulis Acanthopanacis Senticosi 20 parts, Japanese Ardisia Herb 5 parts, Styrax 10 parts, White Mulberry Root-bark 16 parts by following weight part; Mix, be crushed to 60 orders.
Test example: additive of the present invention is to glossy ganoderma antineoplastic action effect
L materials and methods
1.1 sample
The full powder A of glossy ganoderma that culture medium A cultivates out;
The full powder B of the glossy ganoderma that substratum B cultivates out.
Human body recommended intake be ll.7mg/kg.bw/d (0.7 ~ l.4g/d, in 0.7g/d; Human body weight is in 60kg).
Culture medium A, is made up of the raw material of following weight percent: maize spike stalk slag 49.2%, weed tree sawdust 30%, wheat bran 20%, ammonium sulfate about 0.2%, spice water content 70%;
Substratum B, is made up of the raw material of following weight percent: maize spike stalk slag 44.2%, weed tree sawdust 30%, wheat bran 20%, ammonium sulfate about 0.2%, the embodiment of the present invention 3 additive 5%, spice water content 70%;
1.2 tumor-bearing mice
Thered is provided by GuangXi Medicine Institute.
1.3 experimental animal
Male ICR mouse 60, body weight 20 ~ 23g, is provided by Guangxi Medical University's Experimental Animal Center.
Animal is divided into Normal group (giving 0.5% starch fluid) at random, and glossy ganoderma full powder A and the full powder B of glossy ganoderma is 58.5mg/kg.bw/d (being equivalent to 5 times of human body recommended intake ll.7mg/kg.bw/d) separately.Glossy ganoderma full powder A and the full powder B of glossy ganoderma all uses 0.5% starch fluid (boiling, after cooling) to be mixed with suspension.All experimental animals give edible full-valence pellet feed, drinking-water of freely ingesting.
1.4 test method
The gavage volume number that each treated animal gives tested material is 0.02mL/g.bw, l time/d, when gavage is to 20d, and every mouse right fore subcutaneous aseptic inoculation S-l80 sarcoma cell suspension [25% (volume fraction), with normal saline dilution] 0.2mL.Stop gavage after continuing gavage l0d after inoculation, after 3d, cervical dislocation puts to death mouse, and every mouse peels off tumour, weighs after tumor surface blood stains filter paper blots.
1.5 data statistics
With one-way analysis of variance in SAS6.l2 statistical software, the data obtained between each experimental group is compared.
2 test-results
2.1 body weight
Each group of Mouse Weight changing conditions is in Table l.Result shows that glossy ganoderma full powder A and the full powder B of glossy ganoderma respectively organizes Mouse Weight and compares with Normal group, no significant difference (P>0.05).
The full powder A of table 1 glossy ganoderma and B Tumor suppression test mice body weights means standard deviation
Group Number of animals (only) Starting weight (g) 20d body weight (g) 33d body weight (g)
Normal group 20 21.4±0.5 34.5±2.5 31.6±2.3
The full powder A of glossy ganoderma 20 20.9±0.6 33.8±1.9 30.9±2.2
The full powder B of glossy ganoderma 20 21.1±0.7 33.9±2.6 31.7±2.0
Note: during 33d, body weight is for going body weight after knurl.
2.2 suppress the test of S-180 sarcoma
Suppress S-l80 sarcoma to test each group of mouse tumor and heavily the results are shown in Table 2.Experimental data wherein, is means standard deviation.
The full powder of table 2 glossy ganoderma A and B is on the impact of little s-180 sarcoma weight
Group Number of animals (only) Knurl heavy (g)
Normal group 20 1.72±0.16
The full powder A of glossy ganoderma 20 1.21±0.19*
The full powder B of glossy ganoderma 20 0.69±0.25*
Note: * Dunnett'sT checks, and compares and has significance, P<0.05 with Normal group.
Table 2 result shows, glossy ganoderma full powder A and the full powder B of glossy ganoderma has the effect suppressing mouse S-l80 growing sarcoma, but comparatively the full powder A of glossy ganoderma is remarkable for glossy ganoderma full powder B inhibition, illustrates that adding additive of the present invention can significantly improve the antitumor efficacy strengthening glossy ganoderma.

Claims (2)

1. Medium for Ganoderma lucidum specialist additive, is characterized in that, is made up of the raw material of following weight part: Fructus Cnidii 20 ~ 40 parts, pomegranate rind 10 ~ 20 parts, Red Halloysite 5 ~ 10 parts, Root of Glutinous Rice 10 ~ 20 parts, shriveled wheat 5 ~ 10 parts, Semen Astragali Complanati 5 ~ 10 parts, Radix Ephedrae 30 ~ 50 parts, Radix Et Caulis Acanthopanacis Senticosi 10 ~ 20 parts, Japanese Ardisia Herb 5 ~ 11 parts, Styrax 10 ~ 30 parts, White Mulberry Root-bark 6 ~ 16 parts.
2. Medium for Ganoderma lucidum specialist additive according to claim 1, is characterized in that, is made up of the raw material of following weight part: Fructus Cnidii 30 parts, pomegranate rind 15 parts, Red Halloysite 8 parts, Root of Glutinous Rice 15 parts, shriveled wheat 7 parts, Semen Astragali Complanati 8 parts, Radix Ephedrae 40 parts, Radix Et Caulis Acanthopanacis Senticosi 15 parts, Japanese Ardisia Herb 8 parts, Styrax 20 parts, White Mulberry Root-bark 11 parts.
CN201510982318.9A 2015-12-24 2015-12-24 Additive special for lucid ganoderma culture medium Pending CN105384549A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106278401A (en) * 2016-08-31 2017-01-04 安徽黄山云乐灵芝有限公司 A kind of artificial log glossy ganoderma culture medium
CN107244987A (en) * 2017-08-15 2017-10-13 桐城市泰安中药材种植专业合作社 A kind of Medium for Ganoderma lucidum containing Chinese medicine additive
CN107619354A (en) * 2017-11-09 2018-01-23 江西仙芝来生物科技有限公司 A kind of ganoderma lucidum special culture media additive and preparation method thereof

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Publication number Priority date Publication date Assignee Title
JP2001204248A (en) * 2000-01-25 2001-07-31 Hokuto Corp Artificial medium for mushroom cultivation and method of cultivation using the artificial medium
CN102835248A (en) * 2012-08-09 2012-12-26 颍上县鸿涛菌业专业合作社 Method for cultivating lucid ganoderma by using mulberry branches
CN103125273A (en) * 2013-03-14 2013-06-05 安徽省康美来大别山生物科技有限公司 Ganoderma lucidum cultivation method
CN103460990A (en) * 2013-08-05 2013-12-25 滁州市南谯区大柳镇凤胜食用菌专业合作社 Cultivation method for blood-sugar-reduction health-care ganoderma lucidum
CN104030844A (en) * 2014-06-30 2014-09-10 桂林丰茂源农业技术开发有限公司 Lucid ganoderma substitute cultivation medium with common andrographis herb
CN104488551A (en) * 2014-12-18 2015-04-08 芝圣(天津)生物科技有限公司 Ganoderma tsugae strain cultivation method
CN105061031A (en) * 2015-07-31 2015-11-18 桂林宏旺菌业股份有限公司 Mythic Fungus cultivation medium

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001204248A (en) * 2000-01-25 2001-07-31 Hokuto Corp Artificial medium for mushroom cultivation and method of cultivation using the artificial medium
CN102835248A (en) * 2012-08-09 2012-12-26 颍上县鸿涛菌业专业合作社 Method for cultivating lucid ganoderma by using mulberry branches
CN103125273A (en) * 2013-03-14 2013-06-05 安徽省康美来大别山生物科技有限公司 Ganoderma lucidum cultivation method
CN103460990A (en) * 2013-08-05 2013-12-25 滁州市南谯区大柳镇凤胜食用菌专业合作社 Cultivation method for blood-sugar-reduction health-care ganoderma lucidum
CN104030844A (en) * 2014-06-30 2014-09-10 桂林丰茂源农业技术开发有限公司 Lucid ganoderma substitute cultivation medium with common andrographis herb
CN104488551A (en) * 2014-12-18 2015-04-08 芝圣(天津)生物科技有限公司 Ganoderma tsugae strain cultivation method
CN105061031A (en) * 2015-07-31 2015-11-18 桂林宏旺菌业股份有限公司 Mythic Fungus cultivation medium

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106278401A (en) * 2016-08-31 2017-01-04 安徽黄山云乐灵芝有限公司 A kind of artificial log glossy ganoderma culture medium
CN107244987A (en) * 2017-08-15 2017-10-13 桐城市泰安中药材种植专业合作社 A kind of Medium for Ganoderma lucidum containing Chinese medicine additive
CN107619354A (en) * 2017-11-09 2018-01-23 江西仙芝来生物科技有限公司 A kind of ganoderma lucidum special culture media additive and preparation method thereof

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