CN104487573B - 制备用于核酸扩增的样品的方法 - Google Patents

制备用于核酸扩增的样品的方法 Download PDF

Info

Publication number
CN104487573B
CN104487573B CN201380028534.9A CN201380028534A CN104487573B CN 104487573 B CN104487573 B CN 104487573B CN 201380028534 A CN201380028534 A CN 201380028534A CN 104487573 B CN104487573 B CN 104487573B
Authority
CN
China
Prior art keywords
container
nucleic acid
sample
porous solid
solid matrix
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201380028534.9A
Other languages
English (en)
Chinese (zh)
Other versions
CN104487573A (zh
Inventor
克林特·佩雷拉
卡瑟尔约瑟夫·迈凯尔甘恩
劳伦斯卡洛·媞希
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lumora Ltd
Original Assignee
Lumora Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=46160103&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN104487573(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lumora Ltd filed Critical Lumora Ltd
Publication of CN104487573A publication Critical patent/CN104487573A/zh
Application granted granted Critical
Publication of CN104487573B publication Critical patent/CN104487573B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/161—Temperature conditioning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3861—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography using an external stimulus
    • B01D15/3876—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography using an external stimulus modifying the temperature
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201380028534.9A 2012-03-30 2013-03-28 制备用于核酸扩增的样品的方法 Expired - Fee Related CN104487573B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1205769.1A GB201205769D0 (en) 2012-03-30 2012-03-30 Methods for preparing samples for nucleic acid amplification
GB1205769.1 2012-03-30
PCT/GB2013/050846 WO2013144654A1 (en) 2012-03-30 2013-03-28 Methods for preparing samples for nucleic acid amplification

Publications (2)

Publication Number Publication Date
CN104487573A CN104487573A (zh) 2015-04-01
CN104487573B true CN104487573B (zh) 2017-10-27

Family

ID=46160103

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380028534.9A Expired - Fee Related CN104487573B (zh) 2012-03-30 2013-03-28 制备用于核酸扩增的样品的方法

Country Status (12)

Country Link
US (1) US10246735B2 (enExample)
EP (1) EP2831230B1 (enExample)
JP (1) JP6325517B2 (enExample)
CN (1) CN104487573B (enExample)
AU (1) AU2013239408B2 (enExample)
BR (1) BR112014024421A2 (enExample)
CA (1) CA2868927A1 (enExample)
ES (1) ES2622352T3 (enExample)
GB (1) GB201205769D0 (enExample)
IN (1) IN2014DN08573A (enExample)
WO (1) WO2013144654A1 (enExample)
ZA (1) ZA201407220B (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3059312A1 (en) * 2015-02-20 2016-08-24 QIAGEN GmbH Nucleic acid extraction method
GB201519565D0 (en) * 2015-11-05 2015-12-23 Alere San Diego Inc Sample preparation device
KR102394104B1 (ko) * 2016-09-28 2022-05-04 가부시키가이샤 후지미인코퍼레이티드 표면 처리 조성물
WO2018185908A1 (ja) * 2017-04-06 2018-10-11 株式会社島津製作所 磁性体粒子操作用デバイス
KR102071058B1 (ko) * 2017-10-27 2020-03-02 주식회사 창 헬스케어 유전자 증폭을 위한 핵산의 추출 및 전달 자동화 장치 및 방법
CN107586774A (zh) * 2017-11-01 2018-01-16 上海涛济医药科技有限公司 一种常温下长时间口腔拭子dna保存和提取方法
CA3105220A1 (en) * 2018-07-11 2020-01-16 Monsanto Technology Llc Extraction of polynucleotides
EP3663619B1 (en) * 2018-12-07 2022-03-16 Danfoss Power Solutions Aps Thermal relief device
CN110724633A (zh) * 2019-11-11 2020-01-24 浙江汇泽医药科技有限公司 一种微量细胞核酸提取与扩增系统及工艺
US20220099622A1 (en) * 2020-09-30 2022-03-31 Icare Diagnostics International Co. Ltd. Nucleic acid detection kit and nucleic acid detection device
WO2022076196A1 (en) * 2020-10-09 2022-04-14 Takara Bio Usa, Inc. Methods and compositions for preparing nucleic acids for genetic analysis
CL2020002629A1 (es) * 2020-10-12 2021-01-15 Taag Genetics S A Nuevo dispositivo de biología molecular para extracción y purificación de ácidos nucleicos desde diferentes tipos de muestras biológicas que comprende resinas de adsorción
CN113637723B (zh) * 2021-07-09 2024-03-29 江苏康为世纪生物科技股份有限公司 一种粪便样本核酸保存液及其制备方法
EP4163369A1 (en) * 2021-10-08 2023-04-12 AXAGARIUS GmbH & Co. KG Use of mixtures of polyvinylpyrrolidone and ammonium sulfate in the isolation of nucleic acids
EP4463563A1 (en) * 2022-01-10 2024-11-20 Certest Biotec, S.L. Method for processing nucleic-acid containing samples
EP4630581A2 (en) * 2022-12-06 2025-10-15 Darwin Biosciences, Inc. System and apparatus for the hand-held amplification and detection of nucleic acids
US20240410025A1 (en) * 2023-06-09 2024-12-12 Colorado Genomics LLC Method for rapidly and accurately detecting hop latent viroid rna
CN117025379B (zh) * 2023-10-09 2023-12-15 迪飞医学科技(南京)有限公司 一种rapid恒温扩增核酸检测装置以及检测方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999060005A1 (en) * 1998-05-15 1999-11-25 Fsm Technologies Limited Method and device for purifying nucleic acids
WO2003033740A2 (en) * 2001-07-10 2003-04-24 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid
WO2004005553A1 (en) * 2002-07-10 2004-01-15 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid from a sample
EP1512741A2 (en) * 2003-09-03 2005-03-09 Fuji Photo Film Co., Ltd. A method for separation and purification of nucleic acid
CN101568636A (zh) * 2006-11-02 2009-10-28 威腾技术公司 用于诊断性检测的模块

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US555500A (en) 1896-03-03 Josef denk
US1994323A (en) * 1930-08-27 1935-03-12 Inez H Pelrce Coffee extracting device
WO1994003250A1 (en) 1992-08-07 1994-02-17 Akzo Nobel N.V. Process and apparatus for removing bottoms from a mixture
AU2003221888B2 (en) 2002-04-11 2008-11-06 Medimmune, Llc Preservation of bioactive materials by spray drying
CA2521999A1 (en) * 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
JP4399304B2 (ja) 2004-03-29 2010-01-13 富士フイルム株式会社 自動核酸分離精製方法およびその装置
JP2007306867A (ja) 2006-05-19 2007-11-29 Hitachi High-Technologies Corp 核酸抽出装置及び核酸抽出方法
JP5011012B2 (ja) * 2007-07-18 2012-08-29 株式会社日立ハイテクノロジーズ 核酸抽出装置
CN102812118B (zh) 2010-04-08 2016-01-20 恰根有限公司 分离和纯化核酸的方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999060005A1 (en) * 1998-05-15 1999-11-25 Fsm Technologies Limited Method and device for purifying nucleic acids
WO2003033740A2 (en) * 2001-07-10 2003-04-24 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid
WO2004005553A1 (en) * 2002-07-10 2004-01-15 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid from a sample
EP1512741A2 (en) * 2003-09-03 2005-03-09 Fuji Photo Film Co., Ltd. A method for separation and purification of nucleic acid
CN101568636A (zh) * 2006-11-02 2009-10-28 威腾技术公司 用于诊断性检测的模块

Also Published As

Publication number Publication date
CA2868927A1 (en) 2013-10-03
WO2013144654A1 (en) 2013-10-03
JP2015511497A (ja) 2015-04-20
EP2831230B1 (en) 2017-02-01
EP2831230A1 (en) 2015-02-04
JP6325517B2 (ja) 2018-05-16
AU2013239408A1 (en) 2014-10-23
GB201205769D0 (en) 2012-05-16
IN2014DN08573A (enExample) 2015-05-15
ZA201407220B (en) 2017-01-25
CN104487573A (zh) 2015-04-01
ES2622352T3 (es) 2017-07-06
AU2013239408B2 (en) 2018-03-08
US20150080562A1 (en) 2015-03-19
BR112014024421A2 (pt) 2017-07-25
US10246735B2 (en) 2019-04-02

Similar Documents

Publication Publication Date Title
CN104487573B (zh) 制备用于核酸扩增的样品的方法
JP4291247B2 (ja) 核酸を単離する方法
CA2289943C (en) Solid-phase nucleic acid isolation
US9856515B2 (en) Removal of PCR inhibitors
CN102812118B (zh) 分离和纯化核酸的方法
US20160317949A1 (en) Chromatographic device and method for isolating and purifying nucleic acids
EP2942394A1 (en) New method for isolating microbial DNA
JP3979996B2 (ja) 固体支持体を使用してｒｎａを精製するための組成物および方法
JP2010505425A (ja) 細胞を単離する方法およびキット
Lee et al. DNA extraction methods in forensic analysis
WO2015159979A1 (ja) 短鎖核酸の回収方法
JP2011527185A (ja) 混合生物試料のための高速分析法
JP5463492B2 (ja) 微生物細胞からのプラスミドｄｎａ抽出法
EP1524317A1 (en) Methods for isolating nucleic acids
Kapustin et al. High-throughput method of one-step DNA isolation for PCR diagnostics of Mycobacterium tuberculosis
WO2011124703A1 (en) Method for selective isolation and purification of nucleic acids
Minarovičová et al. A method for the detection of Cryptosporidium parvum oocysts in milk based on microfiltration and real-time polymerase chain reaction
AU2020202825B2 (en) Polynucleotide capture materials, and methods of using same
JP2003116550A (ja) 核酸単離方法
JP2006246732A (ja) 核酸精製用支持体および精製方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20171027

Termination date: 20210328