CN104480026A - Production method of auricularia auricularmycelium for auricularia auricular polysaccharide extraction - Google Patents
Production method of auricularia auricularmycelium for auricularia auricular polysaccharide extraction Download PDFInfo
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- CN104480026A CN104480026A CN201410822277.2A CN201410822277A CN104480026A CN 104480026 A CN104480026 A CN 104480026A CN 201410822277 A CN201410822277 A CN 201410822277A CN 104480026 A CN104480026 A CN 104480026A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses aproduction method of auricularia auricularmycelium for auricularia auricular polysaccharide extraction. The auricularia auricularmycelium is obtained through seven steps of weighing, base material pretreatment, mixing, bagging and sterilization, inoculation and culture, spawn running and management as well as bag removal and harvesting. Compared with the prior art, the production method has the advantages as follows: coarse lignocellulose adopting a macromolecular structure is converted into fine lignocellulose adopting a micromolecular structurethrough fermentation pretreatment of a base material, effective utilization of hyphae during culture of the mycelium is facilitated, the biological conversion and utilization rate at the culture stage of themycelium is increased to be about 70%, the culture time of the mycelium is shortened, the production efficiency is improved, the production cost is reduced, waste from industrial and agricultural production is utilized comprehensively, and the environment is protected.
Description
Technical field
The present invention relates to technical field of edible fungi production, in particular a kind of production method of the Auricularia mycelium for extracting Auricularia polysaccharide.
Background technology
Edible mushrooms is due to nutritious, the heath food or functional food of generally acknowledging in the world, not only rich in proteins, VITAMIN, and contained physiologically active substance, as macromolecule polysaccharide, natural organic germanium, cAMP, triterpene compound etc., there is important value to the immunologic function improving human body.
Auricularia auriculajudae is the traditional famous edible mushrooms of China, in the world artificial domesticating cultivation the earliest.Auricularia auriculajudae is nutritious, delicious flavour, has very high health care and pharmaceutical use, is important edible mushrooms and medicinal fungus.Auricularia auriculajudae contains multiple amino acids, VITAMIN and mineral salt and robust fibre etc.
Modern medicine and trophology deepen continuously research, also containing dextran composition in auricularia auriculajudae, claim Auricularia polysaccharide, and have effect that reducing blood-fat reduces cholesterol, its pharmaceutical use is very high.Technology at first extracts Auricularia polysaccharide from auricularia auriculajudae sporophore, the research of modern technologies shows, in the mycelium of plantation auricularia auriculajudae, the content of Auricularia polysaccharide is higher than auricularia auriculajudae sporophore, the extraction of Auricularia polysaccharide just from auricularia auriculajudae sporophore be raw material turn to from the mycelium of auricularia auriculajudae be the mode of production that raw material extracts.From mycelium, extract polysaccharide, there is Mycelium growth rate fast, fermentation step is simple, be easy to the advantages such as control, from mycelium, extract polysaccharide can shorten the production time and enhance productivity.
From Auricularia mycelium, extract Auricularia polysaccharide usually have two kinds of modes, one is that solution fermentation cultivates Auricularia mycelium extraction, this extraction method, complex process, and equipment investment is large, and production cost is high.A kind of is extract from the Auricularia mycelium that solid fermentation method is cultivated, and is engaged in now Auricularia polysaccharide and extracts enterprise, is not utilize in enterprise's tankage of production auricularia auriculajudae sporophore to extract, adopts solid-state Auricularia mycelium to extract exactly.Extract from auricularia auriculajudae sporophore tankage, limit by raw material, limits throughput, main body is still extracted from solid-state Auricularia mycelium.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, providing a kind of production method of the Auricularia mycelium for extracting Auricularia polysaccharide.
The present invention is achieved by the following technical solutions: a kind of production method of the Auricularia mycelium for extracting Auricularia polysaccharide, is characterized in that comprising the following steps:
Step one, weighing, take Auricularia fungus rod after mushroom, beanstalk stalk, wheat bran, urea, white sugar, gypsum by formula;
Step 2, base-material pre-treatment, after the mushroom first step one taken, Auricularia fungus rod and beanstalk stalk are ground into the particle of 0.3 ~ 0.5cm size, and by Auricularia fungus rod after the mushroom after pulverizing and the mixing of beanstalk stalk, 15 ~ 25% cellulase parent things are added in mixture, water use regulation humidity, make water content control 40 ~ 50%, with vinegar acid for adjusting pH to 4.5 ~ 5.5, light pressure in cement pit is inserted floating after mixing thoroughly, keep material plane lower than pond mouth 20 ~ 30cm, cover plastic film, ventage is left in cement pit corner, in pond, temperature controls at 40 ~ 50 DEG C, fermentative degradation is after 3 ~ 5 days, after degrading, base-material takes out, cellulase parent thing obtains by the following method: after mushroom, add the urea of weight ratio 0.5 ~ 0.8%, the cellulase of weight ratio 1 ~ 2% in Auricularia fungus rod, the broken mixture of beanstalk stalk, with vinegar acid for adjusting pH to 4.8 ~ 5.2, water use regulation water content to 40 ~ 50%, mix and be placed in 45 ~ 55 DEG C of thermostat containers, fermentative degradation 70 ~ 90min, obtained Mierocrystalline cellulose proenzyme parent thing.By 5 ~ 10% Mierocrystalline cellulose proenzyme parent things, to add after mushroom in Auricularia fungus rod, beanstalk stalk mixture by above-mentioned degradation process method, carry out biological degradation process, object after biological degradation process first, be the parent thing of next cellulase, the making of primary fiber element proenzyme parent thing, generally can apply mechanically 20 ~ 25 times.
Step 3, mixing, after the degraded that step 2 obtains, add wheat bran, urea, white sugar, gypsum that step one takes in base-material, water use regulation water content to 35 ~ 45%, pH5 ~ 6.5, are uniformly mixed, and make cultivation base material;
Step 4, pack sterilizing, cultivation base material pack obtained for step 3 is made into bacterium rod, bacterium rod upper and lower opening degree of tightness is consistent, the bacterium rod installed is inserted sterilizing kitchen sterilizing, vigorous fire is rapidly heated to 100 DEG C and keeps 48 hours, and same batch of material will pack and is made into bacterium rod and carries out sterilizing within 4 hours;
Step 5, inoculation culture, ceasing fire to be cooled to after below 28 DEG C starts inoculation, will complete with a collection of bacterium rod with a batch inoculation, rod after inoculation is inserted and sends out the vertically and horizontally arranged stacking in bacterium room, coating film, ventilation in early morning every day one hour after leaving standstill a week;
Step 6, hair tube are managed, and within 7 ~ 15 days, do not move bacterium rod after inoculation, control to send out bacterium chambers temp within the scope of 20 ~ 28 DEG C; When mycelia loop diameter reaches 4 ~ 5cm, carry out first time turning acanthopore, each bacterium rod acanthopore 15 ~ 20; Second time acanthopore is carried out, each bacterium rod acanthopore 35 ~ 45 after mycelia is all covered with; Bacterium chambers temp of sending out during acanthopore remains between 25 ~ 28 DEG C, and after turning acanthopore, triangulation stacked by bacterium rod, is highly no more than ten layers;
Step 7, de-bag results, when forming fine and close hypha body in bacterium rod, de-for bacterium rod bag results mycelium, the mycelium of results, can directly enter Auricularia polysaccharide abstraction process, also can be drying for subsequent use.
As further improvement of these options, in step one, after mushroom, the mass ratio of Auricularia fungus rod, beanstalk stalk, wheat bran, urea, white sugar, gypsum is:
Auricularia fungus rod 60 ~ 70 parts after mushroom
Beanstalk stalk 20 ~ 30 parts
5 ~ 10 parts, wheat bran
0.3 ~ 0.5 part, urea
White sugar 1 ~ 1.5 part
Calcium superphosphate 1 ~ 1.5 part
0.5 ~ 1 part, gypsum.
As further improvement of these options, in step one, after mushroom, the mass ratio of Auricularia fungus rod, beanstalk stalk, wheat bran, urea, white sugar, gypsum is:
Auricularia fungus rod 60 parts after mushroom
Beanstalk stalk 30 parts
8 parts, wheat bran
0.4 part, urea
White sugar 1 part
0.6 part, gypsum.
As further improvement of these options, in step 2, in mixture, add cellulase parent thing, cellulase parent thing with pulverize after mushroom after Auricularia fungus rod and the mass ratio of beanstalk stalk mixture be 15% ~ 25%.
As further improvement of these options, in step 2, in mixture, add cellulase parent thing is base-material after degraded, after degrading, base-material is through the acquisition of last round of base-material pre-treatment step, and after degrade rear base-material and the mushroom after pulverizing, Auricularia fungus mass ratio that is excellent and beanstalk stalk mixture is 20%.
The present invention has the following advantages compared to existing technology: improve the bio-transformation utilization ratio at mycelial cultivation stage, by the thin lignocellulose of the rugose wood cellulose conversion small molecule structure of macromolecular structure, be convenient to be that mycelia effectively utilizes when mycelial cultivation.Reach about 70% in mycelial cultivation stage bio-transformation utilization ratio, by biological degradation, be conducive to mycelial growth; shorten the Mycelium culture time, enhance productivity, reduce production cost; the waste of industrial and agricultural production is fully utilized, protection of the environment.
Embodiment
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
For extracting a production method for the Auricularia mycelium of Auricularia polysaccharide, it is characterized in that comprising the following steps:
Step one, weighing, take Auricularia fungus rod after mushroom, beanstalk stalk, wheat bran, urea, white sugar, gypsum by formula, its mass ratio is:
Auricularia fungus rod 60 parts after mushroom
Beanstalk stalk 30 parts
8 parts, wheat bran
0.5 part, urea
White sugar 1 part
0.5 part, gypsum;
Step 2, base-material pre-treatment, after the mushroom first step one taken, Auricularia fungus rod and beanstalk stalk are ground into the particle of 0.3 ~ 0.5cm size, and by Auricularia fungus rod after the mushroom after pulverizing and the mixing of beanstalk stalk, cellulase parent thing is added in mixture, cellulase parent thing with pulverize after mushroom after Auricularia fungus rod and the mass ratio of beanstalk stalk mixture be 1:5, water use regulation humidity, make water content control 40 ~ 50%, with vinegar acid for adjusting pH to 4.5 ~ 5.5, light pressure in cement pit is inserted floating after mixing thoroughly, keep material plane lower than pond mouth 20 ~ 30cm, cover plastic film, ventage is left in cement pit corner, in pond, temperature controls at 40 ~ 50 DEG C, fermentative degradation is after 3 days, after degrading, base-material takes out, cellulase parent thing obtains by the following method: after mushroom, add the urea of weight ratio 0.5 ~ 0.8%, the cellulase of weight ratio 1 ~ 2% in Auricularia fungus rod, the broken mixture of beanstalk stalk, with vinegar acid for adjusting pH to 4.8 ~ 5.2, water use regulation water content to 40 ~ 50%, mix and be placed in 45 ~ 55 DEG C of thermostat containers, fermentative degradation 70 ~ 90min, obtained Mierocrystalline cellulose proenzyme parent thing.By 5 ~ 10% Mierocrystalline cellulose proenzyme parent things, to add after mushroom in Auricularia fungus rod, beanstalk stalk mixture by above-mentioned degradation process method, carry out biological degradation process, object after biological degradation process first, be the parent thing of next cellulase, the making of primary fiber element proenzyme parent thing, generally can apply mechanically 20 ~ 25 times.
In whole auricularia auriculajudae production process, at mycelial cultivation stage, because Mycelium growth rate is fast, the time is short, only have the substrate material of about 30% to carry out bio-transformation utilization, remainder will arrive the fruiting stage could be continued to transform, and transformation efficiency also can only reach about 80%, some mushroom agriculture is according to this characteristic, in the substrate material of the Edible Fungi in the coming year, excellent after adding the mushroom of 10 ~ 20%, do not affect edible mushrooms output.The object of the invention is to produce Auricularia mycelium for extracting Auricularia polysaccharide, after the proportioning of substrate material has just been selected the mushroom that lignocellulose is lower, Auricularia fungus rod, beanstalk stalk are body material, in order to improve in mycelial cultivation stage bio-transformation utilization ratio, biodegradation technique is adopted to carry out degradation treatment to Auricularia fungus rod, beanstalk stalk after mushroom, by the thin lignocellulose of the rugose wood cellulose conversion small molecule structure of macromolecular structure, be convenient to be that mycelia effectively utilizes when mycelial cultivation.Reach about 70% in mycelial cultivation stage bio-transformation utilization ratio, by biological degradation, be conducive to mycelial growth; shorten the Mycelium culture time, enhance productivity, reduce production cost; the waste of industrial and agricultural production is fully utilized, protection of the environment.
Step 3, mixing, after the degraded that step 2 obtains, add wheat bran, urea, white sugar, gypsum that step one takes in base-material, water use regulation water content to 40%, pH5.5, is uniformly mixed, and makes cultivation base material;
Step 4, pack sterilizing, cultivation base material pack obtained for step 3 is made into bacterium rod, bacterium rod upper and lower opening degree of tightness is consistent, the bacterium rod installed is inserted sterilizing kitchen sterilizing, vigorous fire is rapidly heated to 100 DEG C and keeps 48 hours, and same batch of material will pack and is made into bacterium rod and carries out sterilizing within 4 hours;
Step 5, inoculation culture, ceasing fire to be cooled to after below 28 DEG C starts inoculation, will complete with a collection of bacterium rod with a batch inoculation, rod after inoculation is inserted and sends out the vertically and horizontally arranged stacking in bacterium room, coating film, ventilation in early morning every day one hour after leaving standstill a week;
Step 6, hair tube are managed, and within 7 ~ 15 days, do not move bacterium rod after inoculation, control to send out bacterium chambers temp within the scope of 20 ~ 28 DEG C; When mycelia loop diameter reaches 4 ~ 5cm, carry out first time turning acanthopore, each bacterium rod acanthopore 15; Second time acanthopore is carried out, each bacterium rod acanthopore 35 after mycelia is all covered with; Bacterium chambers temp of sending out during acanthopore remains between 25 ~ 28 DEG C, and after turning acanthopore, triangulation stacked by bacterium rod, is highly no more than ten layers;
Step 7, de-bag results, when forming fine and close hypha body in bacterium rod, de-for bacterium rod bag results mycelium, the mycelium of results, can directly enter Auricularia polysaccharide abstraction process, also can be drying for subsequent use.The production process of mycelia, it is exactly the process of accumulation nutrition, ripe fungus mycelium divides mycelium, mycelia kink body, fine and close hypha body and fruit body primordium four-stage, the object of the invention is to produce Auricularia mycelium, at the mycelium water content in harvest that the fine and close hypha body stage is best, therefore when forming fine and close hypha body in bacterium rod, namely start results.
Embodiment 2
Step one, weighing, take Auricularia fungus rod after mushroom, beanstalk stalk, wheat bran, urea, white sugar, gypsum by formula, its mass ratio is:
Auricularia fungus rod 68 parts after mushroom
Beanstalk stalk 20 parts
10 parts, wheat bran
0.5 part, urea
White sugar 0.5 part
1 part, gypsum;
Step 2, base-material pre-treatment, after the mushroom first step one taken, Auricularia fungus rod and beanstalk stalk are ground into the particle of 0.3 ~ 0.5cm size, and by Auricularia fungus rod after the mushroom after pulverizing and the mixing of beanstalk stalk, base-material after degraded is added in mixture, after degrading, the base-material base-material pre-treatment step be through in embodiment 1 obtains, and after degrade rear base-material and the mushroom after pulverizing, the mass ratio of Auricularia fungus rod and beanstalk stalk mixture is 1:4.Water use regulation humidity, make water content control 50%, with vinegar acid for adjusting pH to 4.5 ~ 5.5, insert light pressure in cement pit after mixing thoroughly floating, keep material plane lower than pond mouth 20 ~ 30cm, cover plastic film, ventage is left in cement pit corner, in pond, temperature controls at 40 ~ 50 DEG C, and fermentative degradation is after 5 days, and after degrading, base-material takes out; Base-material after the degraded of general primary fiber element enzyme parent thing process, can be used for circulation degraded 20 ~ 25 times.
In whole auricularia auriculajudae production process, at mycelial cultivation stage, because Mycelium growth rate is fast, the time is short, only have the substrate material of about 30% to carry out bio-transformation utilization, remainder will arrive the fruiting stage could be continued to transform.The object of the invention is to produce Auricularia mycelium for extracting Auricularia polysaccharide, after the proportioning of substrate material has just been selected the mushroom that lignocellulose is lower, Auricularia fungus rod, beanstalk stalk are body material, in order to improve in mycelial cultivation stage bio-transformation utilization ratio, biodegradation technique is adopted to carry out degradation treatment to Auricularia fungus rod, beanstalk stalk after mushroom, by the thin lignocellulose of the rugose wood cellulose conversion small molecule structure of macromolecular structure, be convenient to be that mycelia effectively utilizes when mycelial cultivation.Reach about 70% in mycelial cultivation stage bio-transformation utilization ratio, by biological degradation, be conducive to mycelial growth; shorten the Mycelium culture time, enhance productivity, reduce production cost; the waste of industrial and agricultural production is fully utilized, protection of the environment.
Step 3, mixing, after the degraded that step 2 obtains, add wheat bran, urea, white sugar, gypsum that step one takes in base-material, water use regulation water content to 45%, pH6.0, is uniformly mixed, and makes cultivation base material;
Step 4, pack sterilizing, cultivation base material pack obtained for step 3 is made into bacterium rod, bacterium rod upper and lower opening degree of tightness is consistent, the bacterium rod installed is inserted sterilizing kitchen sterilizing, vigorous fire is rapidly heated to 100 DEG C and keeps 48 hours, and same batch of material will pack and is made into bacterium rod and carries out sterilizing within 4 hours;
Step 5, inoculation culture, ceasing fire to be cooled to after below 28 DEG C starts inoculation, will complete with a collection of bacterium rod with a batch inoculation, rod after inoculation is inserted and sends out the vertically and horizontally arranged stacking in bacterium room, coating film, ventilation in early morning every day one hour after leaving standstill a week;
Step 6, hair tube are managed, and within 7 ~ 15 days, do not move bacterium rod after inoculation, control to send out bacterium chambers temp within the scope of 20 ~ 28 DEG C; When mycelia loop diameter reaches 4 ~ 5cm, carry out first time turning acanthopore, each bacterium rod acanthopore 20; Second time acanthopore is carried out, each bacterium rod acanthopore 45 after mycelia is all covered with; Bacterium chambers temp of sending out during acanthopore remains between 25 ~ 28 DEG C, and after turning acanthopore, triangulation stacked by bacterium rod, is highly no more than ten layers;
Step 7, de-bag results, when forming fine and close hypha body in bacterium rod, de-for bacterium rod bag results mycelium, the mycelium of results, can directly enter Auricularia polysaccharide abstraction process, also can be drying for subsequent use.The production process of mycelia, it is exactly the process of accumulation nutrition, ripe fungus mycelium divides mycelium, mycelia kink body, fine and close hypha body and fruit body primordium four-stage, the object of the invention is to produce Auricularia mycelium, at the mycelium water content in harvest that the fine and close hypha body stage is best, therefore when forming fine and close hypha body in bacterium rod, namely start results.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (5)
1., for extracting a production method for the Auricularia mycelium of Auricularia polysaccharide, it is characterized in that comprising the following steps:
Step one, weighing, take Auricularia fungus rod after mushroom, beanstalk stalk, wheat bran, urea, white sugar, gypsum by formula;
Step 2, base-material pre-treatment, after the mushroom first step one taken, Auricularia fungus rod and beanstalk stalk are ground into the particle of 0.3 ~ 0.5cm size, and by Auricularia fungus rod after the mushroom after pulverizing and the mixing of beanstalk stalk, carry out cellulase biological degradation process and prepare base-material.
Step 3, mixing, after the degraded that step 2 obtains, add wheat bran, urea, white sugar, gypsum that step one takes in base-material, water use regulation water content to 35 ~ 45%, pH5 ~ 6.5, are uniformly mixed, and make cultivation base material;
Step 4, pack sterilizing, cultivation base material pack obtained for step 3 is made into bacterium rod, bacterium rod upper and lower opening degree of tightness is consistent, the bacterium rod installed is inserted sterilizing kitchen sterilizing, vigorous fire is rapidly heated to 100 DEG C and keeps 48 hours, and same batch of material will pack and is made into bacterium rod and carries out sterilizing within 4 hours;
Step 5, inoculation culture, ceasing fire to be cooled to after below 28 DEG C starts inoculation, will complete with a collection of bacterium rod with a batch inoculation, rod after inoculation is inserted and sends out the vertically and horizontally arranged stacking in bacterium room, coating film, ventilation in early morning every day one hour after leaving standstill a week;
Step 6, hair tube are managed, and within 7 ~ 15 days, do not move bacterium rod after inoculation, control to send out bacterium chambers temp within the scope of 20 ~ 28 DEG C; When mycelia loop diameter reaches 4 ~ 5cm, carry out first time turning acanthopore, each bacterium rod acanthopore 15 ~ 20; Second time acanthopore is carried out, each bacterium rod acanthopore 35 ~ 45 after mycelia is all covered with; Bacterium chambers temp of sending out during acanthopore remains between 25 ~ 28 DEG C, and after turning acanthopore, triangulation stacked by bacterium rod, is highly no more than ten layers;
Step 7, de-bag results, when forming fine and close hypha body in bacterium rod, de-for bacterium rod bag results mycelium, the mycelium of results, can directly enter Auricularia polysaccharide abstraction process, also can be drying for subsequent use.
2. the production method of a kind of Auricularia mycelium for extracting Auricularia polysaccharide as claimed in claim 1, it is characterized in that: in described step one, after described mushroom, the mass ratio of Auricularia fungus rod, beanstalk stalk, wheat bran, urea, white sugar, gypsum is:
Auricularia fungus rod 60 ~ 70 parts after mushroom
Beanstalk stalk 20 ~ 30 parts
5 ~ 10 parts, wheat bran
0.3 ~ 0.5 part, urea
White sugar 1 ~ 1.5 part
0.5 ~ 1 part, gypsum.
3. the production method of a kind of Auricularia mycelium for extracting Auricularia polysaccharide as claimed in claim 1, it is characterized in that: in described step 2, base-material is prepared in cellulase biological degradation process, its treatment process is: in mixture, add cellulase parent thing, water use regulation humidity, make water content control 40 ~ 50%, with vinegar acid for adjusting pH to 4.5 ~ 5.5, light pressure in cement pit is inserted floating after mixing thoroughly, keep material plane lower than pond mouth 20 ~ 30cm, cover plastic film, ventage is left in cement pit corner, in pond, temperature controls at 40 ~ 50 DEG C, fermentative degradation takes out for 3 ~ 5 days, prepared by base-material.Described cellulase parent thing is Auricularia fungus rod, beanstalk stalk powder mixture after the mushroom after degraded last time.Obtain by the following method: after mushroom, add the urea of weight ratio 0.5 ~ 0.8%, the cellulase of weight ratio 1 ~ 2% in Auricularia fungus rod, the broken mixture of beanstalk stalk, with vinegar acid for adjusting pH to 4.8 ~ 5.2, water use regulation water content to 40 ~ 50%, mix and be placed in 45 ~ 55 DEG C of thermostat containers, fermentative degradation 70 ~ 90min, obtained Mierocrystalline cellulose proenzyme parent thing.By 5 ~ 10% Mierocrystalline cellulose proenzyme parent things, to add after mushroom in Auricularia fungus rod, beanstalk stalk mixture by above-mentioned degradation process method, carry out biological degradation process, the object after biological degradation process first, be the parent thing of next cellulase.
4. the production method of a kind of Auricularia mycelium for extracting Auricularia polysaccharide as claimed in claim 1, it is characterized in that: in described step 2, the consumption adding cellulase parent thing in mixture is 15 ~ 25%.
5. plant the production method of the Auricularia mycelium for extracting Auricularia polysaccharide as claimed in claim 1, it is characterized in that: in described step 2, add in mixture cellulase parent thing be degraded after base-material, after described degraded base-material be through last round of base-material pre-treatment step obtain.
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| US10533155B2 (en) | 2016-03-01 | 2020-01-14 | Sustainable Bioproducts, Inc. | Filamentous fungal biomats, methods of their production and methods of their use |
| US10851396B2 (en) | 2014-07-03 | 2020-12-01 | The Fynder Group, Inc. | Acidophilic fusarium oxysporum strains, methods of their production and methods of their use |
| US11039635B2 (en) | 2019-02-27 | 2021-06-22 | The Fynder Group, Inc. | Food materials comprising filamentous fungal particles |
| US11118305B2 (en) | 2019-06-18 | 2021-09-14 | The Fynder Group, Inc. | Fungal textile materials and leather analogs |
| US11297866B2 (en) | 2017-08-30 | 2022-04-12 | The Fynder Group, Inc. | Bioreactor system for the cultivation of filamentous fungal biomass |
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