CN104541967A - Production method for extracting mushroom mycelium of lentinan - Google Patents
Production method for extracting mushroom mycelium of lentinan Download PDFInfo
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- CN104541967A CN104541967A CN201410828010.4A CN201410828010A CN104541967A CN 104541967 A CN104541967 A CN 104541967A CN 201410828010 A CN201410828010 A CN 201410828010A CN 104541967 A CN104541967 A CN 104541967A
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- mushroom
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- lentinus edodes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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Abstract
The invention discloses a production method for extracting mushroom mycelium of lentinan. The mushroom mycelium is obtained by the following seven steps: weighing materials; pretreating base materials; mixing; bagging and sterilizing; inoculating and cultivating; carrying out spawn running management; removing a bag and harvesting. Compared with the prior art, the production method disclosed by the invention has the advantages that through fermentation pretreatment on the base materials, coarse lignocellulose with a macromolecular structure is transformed into fine lignocellulose with a micromolecular structure; effective utilization of hyphae during cultivation of the mycelium is facilitated; the biotransformation utilization rate of the mycelium at the cultivation stage is increased, and reaches about 70%; the cultivation time of the mycelium is shortened; the production efficiency is improved; the production cost is reduced; comprehensive utilization of waste in industrial and agricultural production is achieved; the environment is protected.
Description
Technical field
The present invention relates to technical field of edible fungi production, in particular a kind of production method of the shiitake mushroom hypha for extracting lentinan.
Background technology
Edible mushrooms is due to nutritious, the heath food or functional food of generally acknowledging in the world, not only rich in proteins, VITAMIN, and contained physiologically active substance, as macromolecule polysaccharide, natural organic germanium, cAMP, triterpene compound etc., there is important value to the immunologic function improving human body.
Mushroom is the traditional famous edible mushrooms of China, in the world artificial domesticating cultivation the earliest.Mushroom is nutritious, delicious flavour, has very high health care and pharmaceutical use, is regarded as " king in mushroom ", is important edible mushrooms and medicinal fungus.More than 10 seed amino acids that mushroom contains, wherein have more than the 40 kind of enzyme etc. of the amino acid of 7 kinds of needed by human such as Isoleucine, Methionin, phenylalanine, methionine(Met), Threonine, α-amino-isovaleric acid and VITMAIN B1, B2, PP and mineral salt and robust fibre, six large enzymes.
Modern medicine and trophology deepen continuously research, also containing β same clan dextran composition in mushroom, are referred to as lentinan, can strengthen cell immunocompetent, thus the growth of anticancer, pharmaceutical use is very high.Technology at first extracts lentinan from mushroom fruiting body, the research of modern technologies shows, in the mycelium of plantation mushroom, the content of lentinan is higher than mushroom fruiting body, the extraction of lentinan just from mushroom fruiting body be raw material turn to from the mycelium of mushroom be the mode of production that raw material extracts.From mycelium, extract polysaccharide, there is Mycelium growth rate fast, fermentation step is simple, be easy to the advantages such as control, from mycelium, extract polysaccharide can shorten the production time and enhance productivity.
From shiitake mushroom hypha, extract lentinan usually have two kinds of modes, one is that solution fermentation cultivates shiitake mushroom hypha extraction, this extraction method, complex process, and equipment investment is large, and production cost is high.The method of a CN200810019454 preparing lentinan from mushroom cultivating rod, provide a kind of from solution fermentation extract lentinan in test method process, rest on and be engaged in theoretical investigation experimental stage, also do not enter practical stage.A kind of is extract from the shiitake mushroom hypha that solid fermentation method is cultivated, and is engaged in now lentinan and extracts enterprise, be not utilize in enterprise's tankage of production mushroom fruiting body to extract, adopt solid-state shiitake mushroom hypha to extract exactly.Extract from mushroom fruiting body tankage, limit by raw material, limits throughput, main body is still extracted from solid-state shiitake mushroom hypha.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, providing a kind of production method of the shiitake mushroom hypha for extracting lentinan.
The present invention is achieved by the following technical solutions: a kind of production method of the shiitake mushroom hypha for extracting lentinan, is characterized in that comprising the following steps:
Step one, weighing, take lentinus edodes strain stick, corn cob, wheat bran, urea, white sugar, calcium superphosphate and gypsum after mushroom by formula;
Step 2, base-material pre-treatment, after the mushroom first step one taken, lentinus edodes strain stick and corn cob meal are broken into the particle of 0.3-0.5cm size, and by lentinus edodes strain stick after the mushroom after pulverizing and corn cob mixing, cellulase parent thing is added in mixture, water use regulation humidity, make water content control at 40-50%, with vinegar acid for adjusting pH to 4.5-5.5, light pressure in cement pit is inserted floating after mixing thoroughly, keep material plane lower than pond mouth 20-30cm, cover plastic film, ventage is left in cement pit corner, in pond, temperature controls at 40-50 DEG C, after fermentative degradation 3-5 days, after degrading, base-material takes out, cellulase parent thing obtains by the following method: after mushroom, add the urea of weight ratio 0.5-0.8%, the cellulase of weight ratio 1-2% in lentinus edodes strain stick, the broken mixture of corn cob, with vinegar acid for adjusting pH to 4.8-5.2, water use regulation water content is to 40-50%, mix and be placed in 45-55 DEG C of thermostat container, fermentative degradation 70-90min,
Step 3, mixing, after the degraded that step 2 obtains, add wheat bran, urea, white sugar, calcium superphosphate and gypsum that step one takes in base-material, water use regulation water content, to 50-60%, is uniformly mixed, and makes cultivation matrix;
Step 4, pack sterilizing, cultivation matrix pack obtained for step 3 is made into bacterium rod, and bacterium rod upper and lower opening degree of tightness is consistent, and the bacterium rod installed is inserted sterilizing kitchen sterilizing, vigorous fire is rapidly heated to 100 DEG C and keeps 48 hours, and same batch of material will pack and is made into bacterium rod and carries out sterilizing within 4 hours;
Step 5, inoculation culture, ceasing fire to be cooled to after below 28 DEG C starts inoculation, will complete with a collection of bacterium rod with a batch inoculation, rod after inoculation is inserted and sends out the vertically and horizontally arranged stacking in bacterium room, coating film, ventilation in early morning every day one hour after leaving standstill a week;
Step 6, hair tube are managed, and within 7 ~ 15 days, do not move bacterium rod after inoculation, control to send out bacterium chambers temp within the scope of 24-27 DEG C; When mycelia loop diameter reaches 4-5cm, carry out first time turning acanthopore, each bacterium rod acanthopore 15-20; Second time acanthopore is carried out, each bacterium rod acanthopore 35-45 after mycelia is all covered with; Bacterium chambers temp of sending out during acanthopore remains between 25-28 DEG C, and after turning acanthopore, triangulation stacked by bacterium rod, is highly no more than ten layers;
Step 7, de-bag results, when forming fine and close hypha body in bacterium rod, de-for bacterium rod bag results mycelium, the mycelium of results, can directly enter lentinan abstraction process, also can be drying for subsequent use.
As further improvement of these options, in step one, after mushroom, the mass ratio of lentinus edodes strain stick, corn cob, wheat bran, urea, white sugar, calcium superphosphate and gypsum is:
As further improvement of these options, in step one, after mushroom, the mass ratio of lentinus edodes strain stick, corn cob, wheat bran, urea, white sugar calcium superphosphate and gypsum is:
As further improvement of these options, in step 2, in mixture, add cellulase parent thing, cellulase parent thing with pulverize after mushroom after the mass ratio of lentinus edodes strain stick and corn cob mixture be 5%-10%.
As further improvement of these options, in step 2, in mixture, add cellulase parent thing is base-material after degraded, after degrading, base-material is through the acquisition of last round of base-material pre-treatment step, and after degrade rear base-material and the mushroom after pulverizing, the mass ratio of lentinus edodes strain stick and corn cob mixture is 20%.
The present invention has the following advantages compared to existing technology: improve the bio-transformation utilization ratio at mycelial cultivation stage, by the thin lignocellulose of the rugose wood cellulose conversion small molecule structure of macromolecular structure, be convenient to be that mycelia effectively utilizes when mycelial cultivation.Reach about 70% in mycelial cultivation stage bio-transformation utilization ratio, by biological degradation, be conducive to mycelial growth; shorten the Mycelium culture time, enhance productivity, reduce production cost; the waste of industrial and agricultural production is fully utilized, protection of the environment.
Embodiment
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
For extracting a production method for the shiitake mushroom hypha of lentinan, it is characterized in that comprising the following steps:
Step one, weighing, take lentinus edodes strain stick, corn cob, wheat bran, urea, white sugar, calcium superphosphate and gypsum after mushroom by formula, its mass ratio is:
Step 2, base-material pre-treatment, after the mushroom first step one taken, lentinus edodes strain stick and corn cob meal are broken into the particle of 0.3-0.5cm size, and by lentinus edodes strain stick after the mushroom after pulverizing and corn cob mixing, cellulase parent thing is added in mixture, cellulase parent thing with pulverize after mushroom after the mass ratio of lentinus edodes strain stick and corn cob mixture be 5%, water use regulation humidity, make water content control at 40-50%, with vinegar acid for adjusting pH to 4.5-5.5, light pressure in cement pit is inserted floating after mixing thoroughly, keep material plane lower than pond mouth 20-30cm, cover plastic film, ventage is left in cement pit corner, in pond, temperature controls at 40-50 DEG C, fermentative degradation is after 3 days, after degrading, base-material takes out, cellulase parent thing obtains by the following method: after the mushroom of mass ratio 3:1, add the cellulase accounting for the urea of mixture weight than 0.5%, weight ratio 1% in lentinus edodes strain stick and the broken mixture of corn cob, with vinegar acid for adjusting pH to 4.8-5.2, water use regulation water content to 40%, mix and be placed in 45-55 DEG C of thermostat container, fermentative degradation 70min.In whole Lentnus edodes process, at mycelial cultivation stage, because Mycelium growth rate is fast, the time is short, only have the substrate material of about 30% to carry out bio-transformation utilization, remainder will arrive the fruiting stage could be continued to transform.The object of the invention is to provide shiitake mushroom hypha for extracting lentinan, after the proportioning of substrate material has just been selected the mushroom that lignocellulose is lower, lentinus edodes strain stick, corn cob are body material, in order to improve in mycelial cultivation stage bio-transformation utilization ratio, biodegradation technique is adopted to carry out degradation treatment to lentinus edodes strain stick, corn cob after mushroom, by the thin lignocellulose of the rugose wood cellulose conversion small molecule structure of macromolecular structure, be convenient to be that mycelia effectively utilizes when mycelial cultivation.Reach about 70% in mycelial cultivation stage bio-transformation utilization ratio, by biological degradation, be conducive to mycelial growth; shorten the Mycelium culture time, enhance productivity, reduce production cost; the waste of industrial and agricultural production is fully utilized, protection of the environment.
Step 3, mixing, after the degraded that step 2 obtains, add wheat bran, urea, white sugar, calcium superphosphate and gypsum that step one takes in base-material, water use regulation water content to 50%, is uniformly mixed, and makes cultivation matrix;
Step 4, pack sterilizing, cultivation matrix pack obtained for step 3 is made into bacterium rod, and bacterium rod upper and lower opening degree of tightness is consistent, and the bacterium rod installed is inserted sterilizing kitchen sterilizing, vigorous fire is rapidly heated to 100 DEG C and keeps 48 hours, and same batch of material will pack and is made into bacterium rod and carries out sterilizing within 4 hours;
Step 5, inoculation culture, ceasing fire to be cooled to after below 28 DEG C starts inoculation, will complete with a collection of bacterium rod with a batch inoculation, rod after inoculation is inserted and sends out the vertically and horizontally arranged stacking in bacterium room, coating film, ventilation in early morning every day one hour after leaving standstill a week;
Step 6, hair tube are managed, and within 7 ~ 15 days, do not move bacterium rod after inoculation, control to send out bacterium chambers temp within the scope of 24-27 DEG C; When mycelia loop diameter reaches 4-5cm, carry out first time turning acanthopore, each bacterium rod acanthopore 15; Second time acanthopore is carried out, each bacterium rod acanthopore 35 after mycelia is all covered with; Bacterium chambers temp of sending out during acanthopore remains between 25-28 DEG C, and after turning acanthopore, triangulation stacked by bacterium rod, is highly no more than ten layers;
Step 7, de-bag results, when forming fine and close hypha body in bacterium rod, de-for bacterium rod bag results mycelium, the mycelium of results, can directly enter lentinan abstraction process, also can be drying for subsequent use.The production process of mycelia, it is exactly the process of accumulation nutrition, ripe mushroom mycelium divides mycelium, mycelia kink body, fine and close hypha body and fruit body primordium four-stage, the object of the invention is to produce shiitake mushroom hypha, at the four-stage of the mushroom mycelium of maturation, be best mycelium water content in harvest in the fine and close hypha body stage, therefore when forming fine and close hypha body in bacterium rod, namely start results, shorten implantation time as far as possible.
Embodiment 2
Step one, weighing, take lentinus edodes strain stick, corn cob, wheat bran, urea, white sugar, calcium superphosphate and gypsum after mushroom by formula, its mass ratio is:
Step 2, base-material pre-treatment, after the mushroom first step one taken, lentinus edodes strain stick and corn cob meal are broken into the particle of 0.3-0.5cm size, and by lentinus edodes strain stick after the mushroom after pulverizing and corn cob mixing, base-material after degraded is added in mixture, after degrading, the base-material base-material pre-treatment step be through in embodiment 1 obtains, and after degrade rear base-material and the mushroom after pulverizing, the mass ratio of lentinus edodes strain stick and corn cob mixture is 20%.Water use regulation humidity, make water content control 50%, with vinegar acid for adjusting pH to 4.5-5.5, insert light pressure in cement pit after mixing thoroughly floating, keep material plane lower than pond mouth 20-30cm, cover plastic film, ventage is left in cement pit corner, in pond, temperature controls at 40-50 DEG C, and fermentative degradation is after 5 days, and after degrading, base-material takes out; Base-material after the degraded of general primary fiber element enzyme parent thing process, can be used for circulation degraded more than 20-25 time.In whole Lentnus edodes process, at mycelial cultivation stage, because Mycelium growth rate is fast, the time is short, only have the substrate material of about 30% to carry out bio-transformation utilization, remainder will arrive the fruiting stage could be continued to transform.The object of the invention is to provide shiitake mushroom hypha for extracting lentinan, after the proportioning of substrate material has just been selected the mushroom that lignocellulose is lower, lentinus edodes strain stick, corn cob are body material, in order to improve in mycelial cultivation stage bio-transformation utilization ratio, biodegradation technique is adopted to carry out degradation treatment to lentinus edodes strain stick, corn cob after mushroom, by the thin lignocellulose of the rugose wood cellulose conversion small molecule structure of macromolecular structure, be convenient to be that mycelia effectively utilizes when mycelial cultivation.Reach about 70% in mycelial cultivation stage bio-transformation utilization ratio, by biological degradation, be conducive to mycelial growth; shorten the Mycelium culture time, enhance productivity, reduce production cost; the waste of industrial and agricultural production is fully utilized, protection of the environment.
Step 3, mixing, after the degraded that step 2 obtains, add wheat bran, urea, white sugar, calcium superphosphate and gypsum that step one takes in base-material, water use regulation water content to 60%, is uniformly mixed, and makes cultivation matrix;
Step 4, pack sterilizing, cultivation matrix pack obtained for step 3 is made into bacterium rod, and bacterium rod upper and lower opening degree of tightness is consistent, and the bacterium rod installed is inserted sterilizing kitchen sterilizing, vigorous fire is rapidly heated to 100 DEG C and keeps 48 hours, and same batch of material will pack and is made into bacterium rod and carries out sterilizing within 4 hours;
Step 5, inoculation culture, ceasing fire to be cooled to after below 28 DEG C starts inoculation, will complete with a collection of bacterium rod with a batch inoculation, rod after inoculation is inserted and sends out the vertically and horizontally arranged stacking in bacterium room, coating film, ventilation in early morning every day one hour after leaving standstill a week;
Step 6, hair tube are managed, and within 7 ~ 15 days, do not move bacterium rod after inoculation, control to send out bacterium chambers temp within the scope of 24-27 DEG C; When mycelia loop diameter reaches 4-5cm, carry out first time turning acanthopore, each bacterium rod acanthopore 20; Second time acanthopore is carried out, each bacterium rod acanthopore 45 after mycelia is all covered with; Bacterium chambers temp of sending out during acanthopore remains between 25-28 DEG C, and after turning acanthopore, triangulation stacked by bacterium rod, is highly no more than ten layers;
Step 7, de-bag results, when forming fine and close hypha body in bacterium rod, de-for bacterium rod bag results mycelium, the mycelium of results, can directly enter lentinan abstraction process, also can be drying for subsequent use.The production process of mycelia, it is exactly the process of accumulation nutrition, ripe mushroom mycelium divides mycelium, mycelia kink body, fine and close hypha body and fruit body primordium four-stage, the object of the invention is to produce shiitake mushroom hypha, at the four-stage of the mushroom mycelium of maturation, be best mycelium water content in harvest in the fine and close hypha body stage, therefore when forming fine and close hypha body in bacterium rod, namely start results, shorten implantation time as far as possible.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (5)
1., for extracting a production method for the shiitake mushroom hypha of lentinan, it is characterized in that comprising the following steps:
Step one, weighing, take lentinus edodes strain stick, corn cob, wheat bran, urea, white sugar, calcium superphosphate and gypsum after mushroom by formula;
Step 2, base-material pre-treatment, after the mushroom first step one taken, lentinus edodes strain stick and corn cob meal are broken into the particle of 0.3-0.5cm size, and by lentinus edodes strain stick after the mushroom after pulverizing and corn cob mixing, cellulase parent thing is added in mixture, water use regulation humidity, make water content control at 40-50%, with vinegar acid for adjusting pH to 4.5-5.5, light pressure in cement pit is inserted floating after mixing thoroughly, keep material plane lower than pond mouth 20-30cm, cover plastic film, ventage is left in cement pit corner, in pond, temperature controls at 40-50 DEG C, after fermentative degradation 3-5 days, after degrading, base-material takes out, described cellulase parent thing obtains by the following method: after mushroom, add the urea of weight ratio 0.5-0.8%, the cellulase of weight ratio 1-2% in lentinus edodes strain stick, the broken mixture of corn cob, with vinegar acid for adjusting pH to 4.8-5.2, water use regulation water content is to 40-50%, mix and be placed in 45-55 DEG C of thermostat container, fermentative degradation 70-90min,
Step 3, mixing, after the degraded that step 2 obtains, add wheat bran, urea, white sugar, calcium superphosphate and gypsum that step one takes in base-material, water use regulation water content, to 50-60%, is uniformly mixed, and makes cultivation matrix;
Step 4, pack sterilizing, cultivation matrix pack obtained for step 3 is made into bacterium rod, and bacterium rod upper and lower opening degree of tightness is consistent, and the bacterium rod installed is inserted sterilizing kitchen sterilizing, vigorous fire is rapidly heated to 100 DEG C and keeps 48 hours, and same batch of material will pack and is made into bacterium rod and carries out sterilizing within 4 hours;
Step 5, inoculation culture, ceasing fire to be cooled to after below 28 DEG C starts inoculation, will complete with a collection of bacterium rod with a batch inoculation, rod after inoculation is inserted and sends out the vertically and horizontally arranged stacking in bacterium room, coating film, ventilation in early morning every day one hour after leaving standstill a week;
Step 6, hair tube are managed, and within 7 ~ 15 days, do not move bacterium rod after inoculation, control to send out bacterium chambers temp within the scope of 24-27 DEG C; When mycelia loop diameter reaches 4-5cm, carry out first time turning acanthopore, each bacterium rod acanthopore 15-20; Second time acanthopore is carried out, each bacterium rod acanthopore 35-45 after mycelia is all covered with; Bacterium chambers temp of sending out during acanthopore remains between 25-28 DEG C, and after turning acanthopore, triangulation stacked by bacterium rod, is highly no more than ten layers;
Step 7, de-bag results, when forming fine and close hypha body in bacterium rod, de-for bacterium rod bag results mycelium, the mycelium of results, can directly enter lentinan abstraction process, also can be drying for subsequent use.
2. the production method of a kind of shiitake mushroom hypha for extracting lentinan as claimed in claim 1, it is characterized in that: in described step one, after described mushroom, the mass ratio of lentinus edodes strain stick, corn cob, wheat bran, urea, white sugar, calcium superphosphate and gypsum is:
Lentinus edodes strain stick 60-70 part after mushroom
Corn cob 20-30 part
Wheat bran 5-10 part
0.3 part, urea
White sugar 1-1.5 part
Calcium superphosphate 1-1.5 part
Gypsum 0.5-1 part.
3. the production method of a kind of shiitake mushroom hypha for extracting lentinan as claimed in claim 2, it is characterized in that: in described step one, after described mushroom, the mass ratio of lentinus edodes strain stick, corn cob, wheat bran, urea, white sugar, calcium superphosphate and gypsum is:
Lentinus edodes strain stick 60 parts after mushroom
Corn cob 20 parts
5 parts, wheat bran
0.3 part, urea
White sugar 1 part
Calcium superphosphate 1 part
0.5 part, gypsum.
4. plant the production method of the shiitake mushroom hypha for extracting lentinan as claimed in claim 1, it is characterized in that: in described step 2, in mixture, add cellulase parent thing, described cellulase parent thing with pulverize after mushroom after the mass ratio of lentinus edodes strain stick and corn cob mixture be 5%-10%.
5. plant the production method of the shiitake mushroom hypha for extracting lentinan as claimed in claim 1, it is characterized in that: in described step 2, in mixture, add cellulase parent thing is base-material after degraded, after described degraded, base-material is through that last round of base-material pre-treatment step obtains, after described degraded base-material with pulverize after mushroom after the mass ratio of lentinus edodes strain stick and corn cob mixture be 20%.
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| CN106083242A (en) * | 2016-06-07 | 2016-11-09 | 李习君 | A kind of Cortex cocois radicis garbage culture matrix for cultivating champignon and preparation method thereof |
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| CN107879810A (en) * | 2017-12-06 | 2018-04-06 | 辽宁省农业科学院 | A kind of hickory chick Cultivar culture medium and its preparation method and application |
| CN110923281A (en) * | 2019-12-09 | 2020-03-27 | 黑龙江菇友生物科技开发有限公司 | Edible fungus polysaccharide extraction method, edible fungus polysaccharide and edible fungus beverage |
| CN117099608A (en) * | 2023-09-12 | 2023-11-24 | 西昌学院 | Tobacco waste composting pretreatment method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106083242A (en) * | 2016-06-07 | 2016-11-09 | 李习君 | A kind of Cortex cocois radicis garbage culture matrix for cultivating champignon and preparation method thereof |
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| CN107879810A (en) * | 2017-12-06 | 2018-04-06 | 辽宁省农业科学院 | A kind of hickory chick Cultivar culture medium and its preparation method and application |
| CN110923281A (en) * | 2019-12-09 | 2020-03-27 | 黑龙江菇友生物科技开发有限公司 | Edible fungus polysaccharide extraction method, edible fungus polysaccharide and edible fungus beverage |
| CN110923281B (en) * | 2019-12-09 | 2023-04-07 | 黑龙江菇友生物科技开发有限公司 | Edible fungus polysaccharide extraction method, edible fungus polysaccharide and edible fungus beverage |
| CN117099608A (en) * | 2023-09-12 | 2023-11-24 | 西昌学院 | Tobacco waste composting pretreatment method |
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| CN104541967B (en) | 2016-10-05 |
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