CN104450659A - 使用稳定辅酶的脱氢酶稳定作用 - Google Patents

使用稳定辅酶的脱氢酶稳定作用 Download PDF

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Publication number
CN104450659A
CN104450659A CN201410680334.8A CN201410680334A CN104450659A CN 104450659 A CN104450659 A CN 104450659A CN 201410680334 A CN201410680334 A CN 201410680334A CN 104450659 A CN104450659 A CN 104450659A
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CN
China
Prior art keywords
enzyme
coenzyme
stable
dehydrogenase
nad
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Pending
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CN201410680334.8A
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English (en)
Chinese (zh)
Inventor
D.海因德尔
C.霍恩
C.加斯勒迪切
J.霍内斯
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CN104450659A publication Critical patent/CN104450659A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01006—Glycerol dehydrogenase (1.1.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01028—D-Lactate dehydrogenase (1.1.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01037—Malate dehydrogenase (1.1.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01005—L-Amino-acid dehydrogenase (1.4.1.5)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201410680334.8A 2008-02-19 2009-02-19 使用稳定辅酶的脱氢酶稳定作用 Pending CN104450659A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08003054A EP2093284A1 (de) 2008-02-19 2008-02-19 Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
EP08003054.7 2008-02-19

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN200980105640.6A Division CN101945999B (zh) 2008-02-19 2009-02-19 使用稳定辅酶的脱氢酶稳定作用

Publications (1)

Publication Number Publication Date
CN104450659A true CN104450659A (zh) 2015-03-25

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ID=39768713

Family Applications (3)

Application Number Title Priority Date Filing Date
CN201410680334.8A Pending CN104450659A (zh) 2008-02-19 2009-02-19 使用稳定辅酶的脱氢酶稳定作用
CN200980105640.6A Expired - Fee Related CN101945999B (zh) 2008-02-19 2009-02-19 使用稳定辅酶的脱氢酶稳定作用
CN2010800085457A Pending CN102325896A (zh) 2008-02-19 2010-02-12 干化学层中具有低活性的酶的快速反应动力学

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN200980105640.6A Expired - Fee Related CN101945999B (zh) 2008-02-19 2009-02-19 使用稳定辅酶的脱氢酶稳定作用
CN2010800085457A Pending CN102325896A (zh) 2008-02-19 2010-02-12 干化学层中具有低活性的酶的快速反应动力学

Country Status (15)

Country Link
US (2) US9896666B2 (https=)
EP (2) EP2093284A1 (https=)
JP (2) JP5758128B2 (https=)
KR (3) KR20120134142A (https=)
CN (3) CN104450659A (https=)
AR (1) AR070436A1 (https=)
AU (1) AU2009216908B9 (https=)
BR (1) BRPI0907839A2 (https=)
CA (1) CA2721718C (https=)
HK (1) HK1208703A1 (https=)
MX (1) MX2010008920A (https=)
RU (1) RU2499834C2 (https=)
TW (1) TWI482860B (https=)
WO (1) WO2009103540A1 (https=)
ZA (1) ZA201006638B (https=)

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EP2398909B1 (de) 2009-02-19 2015-07-22 F. Hoffmann-La Roche AG Schnelle reaktionskinetik von enzymen mit niedriger aktivität in trockenen chemieschichten
CA2750474C (en) * 2009-02-19 2017-07-25 F. Hoffmann-La Roche Ag Fast reaction kinetics of enzymes having low activity in dry chemistry layers
CN102325496B (zh) 2009-02-19 2015-10-07 霍夫曼-拉罗奇有限公司 分析辅助工具的节省空间的储存
EP2226007A1 (de) 2009-02-19 2010-09-08 Roche Diagnostics GmbH Testelementmagazin mit abgedeckten Testfeldern
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MX2012006706A (es) * 2009-12-16 2012-07-03 Hoffmann La Roche Deteccion de la descomposicion de las enzimas en un elemento de prueba mediante la liberacion controlada de analito protegido.
EP2636750A1 (en) * 2012-03-06 2013-09-11 Roche Diagniostics GmbH Compatible solute ectoine as well as derivatives thereof for enzyme stabilization
RU2604166C2 (ru) 2012-06-22 2016-12-10 Ф.Хоффманн-Ля Рош Аг Способ и устройство для определения аналита в физиологической жидкости
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EP2994536B1 (en) 2013-05-08 2017-06-14 Roche Diabetes Care GmbH Stabilization of enzymes by nicotinic acid
HK1213296A1 (zh) * 2013-06-04 2016-06-30 豪夫迈‧罗氏有限公司 用於fret的新化合物及与其相关的方法
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EP3074524B1 (en) 2013-11-27 2019-11-06 Roche Diabetes Care GmbH Composition comprising up-converting phosphors for detecting an analyte
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CN107779459B (zh) * 2016-08-31 2021-06-08 安琪酵母股份有限公司 葡萄糖脱氢酶dna分子、载体和菌株及应用
KR102372113B1 (ko) 2016-10-05 2022-03-07 에프. 호프만-라 로슈 아게 다중 분석물 진단 테스트 엘리먼트들을 위한 검출 시약들 및 전극 배열들, 그리고 그것을 사용하는 방법들
EP3339431A1 (en) 2016-12-22 2018-06-27 Roche Diabetes Care GmbH Glucose dehydrogenase variants with improved properties
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Also Published As

Publication number Publication date
JP6113405B2 (ja) 2017-04-12
CN101945999B (zh) 2016-06-08
AU2009216908B2 (en) 2012-04-19
KR20130081300A (ko) 2013-07-16
JP2012517816A (ja) 2012-08-09
US11220674B2 (en) 2022-01-11
EP2245152A1 (de) 2010-11-03
KR20120134142A (ko) 2012-12-11
US9896666B2 (en) 2018-02-20
BRPI0907839A2 (pt) 2015-08-11
AU2009216908B9 (en) 2012-07-05
TW200951222A (en) 2009-12-16
HK1208703A1 (en) 2016-03-11
AR070436A1 (es) 2010-04-07
CN101945999A (zh) 2011-01-12
MX2010008920A (es) 2010-09-07
EP2245152B1 (de) 2019-05-01
CN102325896A (zh) 2012-01-18
RU2499834C2 (ru) 2013-11-27
US20180265849A1 (en) 2018-09-20
ZA201006638B (en) 2011-06-29
KR20100118995A (ko) 2010-11-08
CA2721718A1 (en) 2009-08-27
CA2721718C (en) 2017-11-28
KR101381004B1 (ko) 2014-04-08
AU2009216908A1 (en) 2009-08-27
US20110143416A1 (en) 2011-06-16
WO2009103540A1 (de) 2009-08-27
JP2011514153A (ja) 2011-05-06
TWI482860B (zh) 2015-05-01
RU2010138610A (ru) 2012-03-27
JP5758128B2 (ja) 2015-08-05
EP2093284A1 (de) 2009-08-26

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