CN104396806A - Parastichopus californicus artificial spawning method - Google Patents
Parastichopus californicus artificial spawning method Download PDFInfo
- Publication number
- CN104396806A CN104396806A CN201410549293.9A CN201410549293A CN104396806A CN 104396806 A CN104396806 A CN 104396806A CN 201410549293 A CN201410549293 A CN 201410549293A CN 104396806 A CN104396806 A CN 104396806A
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- China
- Prior art keywords
- ovulation
- sea cucumber
- parastichopus
- californicus
- red ginseng
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a parastichopus californicus artificial spawning method comprising the specific steps that: when parastichopus californicus gonad index exceeds 15%, a 1% spirulina liquid is added and well mixed into a parastichopus californicus culturing tank for carrying out culturing; after 10-15min, male parastichopus californicus are fetched and placed in a spermiation tank for spermiation; after 20-30min, female parastichopus californicus are fetched and placed in an ovulation tank for ovulation; sperms and ovules are collected, and artificial insemination is carried out. Clean seawater is arranged in the spermiation tank and the ovulation tank. With the above parastichopus californicus artificial maturing method, male spermiation and female ovulation can be effectively stimulated, such that spermiation and ovulation synchronization is realized. The method can be applied in nursing productions of different scales. Also, the spirulina liquid is convenient to prepare. The application of spirulina liquid is easier to operate than shade-drying, flowing water, and temperature-increase stimulations.
Description
Technical field
The present invention relates to red ginseng artificial culture field, California, be specifically related to the method for a kind of California red ginseng artificial induced spawning.
Background technology
California red ginseng (one of sea cucumber) originates in North America, all distribution is had to 249 meters of depth of waters from California to intertidal zone, Alaska shallow water, this sea cucumber kind is British Columbia, Canada, the State of Washington of the U.S. and the Important Economic species of Alaska State, and product is mainly sold in China, the U.S., Japan, Korea S and other places.This sea cucumber wild population natural propagation occurs over just North America local 7-8 month, is still in the exploratory stage at present for this kind of sea cucumber parent accelerating and seedling growing process in its original producton location.Therefore, the method for accelerating is very important fast throughout the year to develop parent, also for new varieties introducing in the future, technology export are laid a solid foundation.
Summary of the invention
The object of the present invention is to provide the method for a kind of California red ginseng artificial induced spawning, it can effectively realize manually hastening parturition to California red ginseng, realizes the long-term breeding of California red ginseng.
For achieving the above object, the present invention adopts following technical scheme to implement:
The method of a kind of California red ginseng artificial induced spawning, concrete operations are: when sea cucumber genital gland indices is more than 15%, the spirulina liquid adding 1% in the breeding trough of culturing sea cucumbers stirs evenly to be cultivated, after 10-15min, male sea cucumber taking-up is placed in ejaculation groove and carries out ejaculation, after 20-30min, female sea cucumber is taken out and ovulate as in ovulation groove, collecting smart ovum and carry out artificial insemination, is clean seawater in ejaculation groove and ovulation groove.The spirulina liquid of 1% refers to that the mass concentration of spirulina in spirulina liquid is 1%.
Further scheme is:
Also add the Chaetoceros culture fluid being in Exponential growth stage while adding spirulina liquid, in the Chaetoceros culture fluid of Exponential growth stage, Chaetoceros density is 5.0-6.0 × 10
6cells/ml.Spirulina liquid adds that water temperature raises suddenly 4 DEG C, and Chaetoceros culture fluid and rising water temperature can significantly improve ejaculation ovulation sea cucumber individual amount respectively.
Concrete scheme is:
The condition that sea cucumber cultivates in breeding trough is: temperature of cultivation is 14 DEG C, and fluctuation is no more than 1 DEG C, salinity 31-32, pH 7.8-8.0, water velocity 2L.min up and down
-1, periodicity of illumination is 16:8 (L:D), intensity of illumination 2000Lux.Clear up residual bait ight soil in breeding trough before adding spirulina liquid, stop flowing water.Sea cucumber genital gland indices adds spirulina liquid when being 15%-20%.Be more specifically: when sea cucumber genital gland indices is 9.1-19.6%, add spirulina liquid.
Beneficial effect of the present invention is: adopt such scheme to carry out artificial repening to California red ginseng, its can the male and female sea cucumber ejaculation of effective stimulus, ovulation, realize the synchronism of ejaculation, ovulation, can be applicable to the nursery production of different scale scope; The spirulina liquid preparation used in addition is convenient, comparatively dries in the shade, flowing water, intensification stimulate and more easily operate enforcement.
Embodiment
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is specifically described.Should be appreciated that following word only in order to describe one or more concrete embodiments of the present invention, considered critical is not carried out to the protection domain that the present invention specifically asks.
In following embodiment 1-3, sea cucumber used is economize in Canadian BC in June, 2011 to gather, and picking individual is healthy and strong, and without wound, specification is that 400-600g weight in wet base sea cucumber is placed on wet towel, and incubator carefully transports in seedling rearing room the tank or cement pit of supporting temporarily in sterilizing in advance.Embodiment 1-3 carries out in local seedling rearing room, and the time of hastening parturition in embodiment 1-3, midmorning, clears up residual bait ight soil, stops flowing water before adding spirulina liquid, and seawater adopts husky filter, ultraviolet disinfection.In the Chaetoceros culture fluid of Exponential growth stage, Chaetoceros density is 5.0-6.0 × 10
6cells/ml.
Embodiment 1
Cultivate in 400L breeding trough, temperature of cultivation is 14 DEG C, and fluctuation is no more than 1 DEG C, salinity 31-32, pH 7.8-8.0, water velocity 2L.min up and down
-1, periodicity of illumination is 16:8 (L:D), intensity of illumination 2000Lux, and seawater adopts husky filter, ultraviolet disinfection.Parental mean genital gland indices is 9.1%-19.6%, and sexual maturity seed ginseng ratio is about 50%.Adding in breeding trough with the Chaetoceros liquid 2L of 1% spirulina liquid 2L, Exponential growth stage (makes spirulina mass concentration final in breeding trough reach 0.5%), sea cucumber parent starts to assume an air of complacency immediately, and start to assemble to pool wall, after 10-15min, ripe male sea cucumber starts ejaculation, and after 20-30min, female sea cucumber starts ovulation.Ejaculation and the individual taking-up respectively of ovulation are put into the ejaculation groove filling clean seawater and carries out ejaculation and ovulation with ovulation groove, ejaculation and ovulation are not interrupted because moving, and ejaculation, the smart ovum of rear collection of having ovulated carry out manually accurately fertilization.
Embodiment 2
Cultivate in 400L breeding trough, temperature of cultivation is 14 DEG C, and fluctuation is no more than 1 DEG C, salinity 31-32, pH 7.8-8.0, water velocity 2L.min up and down
-1, periodicity of illumination is 16:8 (L:D), intensity of illumination 2000Lux.Parental mean genital gland indices is 15%-20%, sexual maturity seed ginseng ratio is about 50%, and the spirulina liquid 3L adding 1% in breeding trough stirs evenly, and namely parent sea cucumber occurs phenomenon of assuming an air of complacency, and slowly assemble to pool wall, malely after about 10-15min get final product ejaculation, during ejaculation, male is taken out and be placed in ejaculation groove ejaculation, collect sperm, after about 20-30min, female beginning ovulates, careful taking-up is put into ovulation groove and is ovulated, recovery of ova, and female about 20min ovulation is complete.Ejaculation, the smart ovum of rear collection of having ovulated can carry out artificial accurately fertilization.
Embodiment 3
Cultivate in 400L breeding trough, temperature of cultivation is 14 DEG C, and fluctuation is no more than 1 DEG C, salinity 31-32, pH 7.8-8.0, water velocity 2L.min up and down
-1, periodicity of illumination is 16:8 (L:D), intensity of illumination 2000Lux.Parental mean genital gland indices is 15%-20%, sexual maturity seed ginseng ratio is about 50%, the spirulina liquid 2-3L adding 1% in breeding trough stirs evenly, add the Chaetoceros culture fluid 1-2L of Exponential growth stage simultaneously, to cultivate trough inner water temperature rise 4 DEG C rapidly, namely there is phenomenon of assuming an air of complacency in parent sea cucumber, and slowly assemble to pool wall, malely after about 10-15min get final product ejaculation, during ejaculation, male is taken out and be placed in ejaculation groove ejaculation, collect sperm, after about 20-30min, female beginning ovulates, careful taking-up is put into ovulation groove and is ovulated, recovery of ova, female about 20min ovulation is complete.Ejaculation, the smart ovum of rear collection of having ovulated can carry out artificial accurately fertilization.
Embodiment 4
Cultivate in 400L breeding trough, temperature of cultivation is 14 DEG C, and fluctuation is no more than 1 DEG C, salinity 31-32, pH 7.8-8.0, water velocity 2L.min up and down
-1, periodicity of illumination is 16:8 (L:D), intensity of illumination 2000Lux.Parental mean genital gland indices is 15%-20%, sexual maturity seed ginseng ratio is about 50%, the spirulina liquid 2-3L adding 1% in breeding trough stirs evenly, to cultivate trough inner water temperature rise 4 DEG C rapidly, namely there is phenomenon of assuming an air of complacency in parent sea cucumber, and slowly assemble to pool wall, malely after about 10-15min get final product ejaculation, during ejaculation, male is taken out and be placed in ejaculation groove ejaculation, collect sperm, after about 20-30min, female beginning ovulates, and careful taking-up is put into ovulation groove and ovulated, recovery of ova, female about 20min ovulation is complete.Ejaculation, the smart ovum of rear collection of having ovulated can carry out artificial accurately fertilization.
By above-described embodiment, economize successful incubation at Canadian BC and go out 4 batches of California red ginseng young, larva survive ratio reaches more than 80%, well-grown.
The above is only the preferred embodiment of the present invention; should be understood that; for those skilled in the art; to know in the present invention after contents; under the premise without departing from the principles of the invention; can also make some equal conversion to it and substitute, these convert on an equal basis and substitute and also should be considered as belonging to protection scope of the present invention.
Claims (7)
1. the method for California red ginseng artificial induced spawning, concrete operations are: when sea cucumber genital gland indices is more than 15%, the spirulina liquid adding 1% in the breeding trough of culturing sea cucumbers stirs evenly to be cultivated, after 10-15min, male sea cucumber taking-up is placed in ejaculation groove and carries out ejaculation, after 20-30min, female sea cucumber is taken out and ovulate as in ovulation groove, collecting smart ovum and carry out artificial insemination, is clean seawater in ejaculation groove and ovulation groove.
2. the method for California according to claim 1 red ginseng artificial induced spawning, is characterized in that: also add the Chaetoceros culture fluid being in Exponential growth stage while adding spirulina liquid.
3. the method for California according to claim 1 and 2 red ginseng artificial induced spawning, is characterized in that: after adding spirulina liquid, the water temperature in breeding trough is raised rapidly 4 DEG C.
4. the method for California according to claim 1 and 2 red ginseng artificial induced spawning, it is characterized in that: the condition that sea cucumber cultivates in breeding trough is: temperature of cultivation is 14 DEG C, fluctuation is no more than 1 DEG C, salinity 31-32 up and down, pH7.8-8.0, water velocity 2L.min
-1, periodicity of illumination is 16:8 (L:D), intensity of illumination 2000Lux.
5. the method for California according to claim 2 red ginseng artificial induced spawning, is characterized in that: add spirulina liquid when sea cucumber genital gland indices is 15%-20%.
6. the method for California according to claim 1 and 2 red ginseng artificial induced spawning, is characterized in that: clear up residual bait ight soil in breeding trough before adding spirulina liquid, stop flowing water.
7. the method for California according to claim 5 red ginseng artificial induced spawning, is characterized in that: add spirulina liquid when sea cucumber genital gland indices is 9.1-19.6%.
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| CN201410549293.9A CN104396806A (en) | 2014-10-17 | 2014-10-17 | Parastichopus californicus artificial spawning method |
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| CN201410549293.9A CN104396806A (en) | 2014-10-17 | 2014-10-17 | Parastichopus californicus artificial spawning method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106912419A (en) * | 2017-03-22 | 2017-07-04 | 青岛农业大学 | A kind of artificial induced spawning technology of California sea cucumber |
| CN106962242A (en) * | 2017-03-24 | 2017-07-21 | 青岛农业大学 | A kind of method of California sea cucumber seed breeding |
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2014
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106912419A (en) * | 2017-03-22 | 2017-07-04 | 青岛农业大学 | A kind of artificial induced spawning technology of California sea cucumber |
| CN106962242A (en) * | 2017-03-24 | 2017-07-21 | 青岛农业大学 | A kind of method of California sea cucumber seed breeding |
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Application publication date: 20150311 |
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