CN104350918B - A kind of method of cottage propagation Radix Psammosilenes - Google Patents
A kind of method of cottage propagation Radix Psammosilenes Download PDFInfo
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- CN104350918B CN104350918B CN201410595186.XA CN201410595186A CN104350918B CN 104350918 B CN104350918 B CN 104350918B CN 201410595186 A CN201410595186 A CN 201410595186A CN 104350918 B CN104350918 B CN 104350918B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
A kind of cottage propagation Radix Psammosilenes, first scheme carries out the cultivation again of tender stem segments to Radix Psammosilenes female parent in implementing, until tender stem segments grow to 3 8cm long time, with a pair band axillalry bud, Duan Weiyi cuttage unit of stem of blade, intercept all cutting.The material of intercepting is inserted on wire gauze successively, makes cutting lower end stem section part be soaked in culture fluid, in time soaking stem section and occur expanding, taking out cutting, cuttage, on the seedbed that pre-configured and process are sterilized, treats sprouting of lateral bud, when alabastrum occurs, remove alabastrum in time.The present invention can substantially accelerate the speed of Radix Psammosilenes breeding, shortens growing-seedling period, improves seedling quality, and the technical program provides technology for quickening Radix Psammosilenes seeling industry and ensures, significant to bio-diversity conservation.
Description
Technical field
A kind of method that the present invention relates to cottage propagation Radix Psammosilenes, belongs to biological technical field.
Background technology
Radix Psammosilenes be Caryophyllaceae Radix Psammosilenes platymiscium Radix Psammosilenes (Psammosilene
tunicoidesW.C.Wu et C.Y.Wu), have another name called RADIX PSAMMOSILENE, Kunming Radix Adenophorae (Radix Glehniae), Rhizoma cypripedii macranthi, to leaf seven etc., be distributed mainly on Yunnan, Sichuan, Guizhou and some areas, Tibet.For Chinese distinctive autogenus plant, it is to study pinkwort system classification and evolution and important material thereof.Medication history among the people is the longest, head is loaded in " the southern regions of the Yunnan Province book on Chinese herbal medicine ", then record in the version Pharmacopoeia of the People's Republic of China in 1977, the effects such as tool dissipating blood stasis, detumescence, evacuation of pus, hemostasis, cure mainly rheumatalgia, traumatic injury, hemorrhage, the detumescence and apocenosis of wound surface etc., now commonly use raw material for the pharmacy corporation such as YUNNAN BAIYAO, Guizhou Tai Ji.Along with the expansion of the market demand, Radix Psammosilenes wild resource has met with unprecedented destruction, and excavating of transition result in the in imminent danger of wild resource, has been listed in Chinese Second Class Key Protected Plant in " Chinese Plants Red Data Book ".
Starting to budding after Radix Psammosilenes usually 10 pairs of leaves, along with the continuous eruption of new stolon, bloom while result, florescence, really phase length are longer, seed maturity is inconsistent, more difficulty of collecting seed, and mature seed is without rest period, condition suitably gets final product rudiment, if collecting seed not in time, seed subjects to worm, the plague of rats.According to pertinent literature, Radix Psammosilenes seed germination rate is only 37%-56%, and the germination rate of this reduced levels seriously constrains Radix Psammosilenes implantation in large scale.In recent years, Radix Psammosilenes price rises steadily, and its resource suffers that transition is excavated, and Radix Psammosilenes integrated distribution ground has been difficult to find in existing field, and only partially there is the fragmentary distribution of Radix Psammosilenes remaining in district.In order to preferably utilize this rare natural resources of Chinese medicinal materials, accomplish that protection develops simultaneously with exploitation, this research utilizes band leaf tender stem cuttage Radix Psammosilenes resource is carried out efficient Fast-propagation, good to obtaining concordance within a short period of time, Radix Psammosilenes seedling that stock number is big.Through consulting literatures, Radix Psammosilenes is utilized Agrobacterium rhizogenes carry out hairy root induction and obtain certain effect by existing related scientific research personnel；To Radix Psammosilenes organic principle, enter blood component, tissue-culturing quick-propagation has carried out corresponding research and obtains corresponding achievement, but Radix Psammosilenes is used band leaf tender stem cuttage, have not yet to see report, in order to improve the structure of Radix Psammosilenes cuttage and quick-propagation system, this research proposes first under the conditions of manual intervention, utilizes Radix Psammosilenes tender stem cottage propagation so that it is can quickly form new plant body, thus solve Radix Psammosilenes and utilize seminal propagation speed relatively slow, and by the bigger problem of Environmental Factors.The present invention there is not yet has similar or identical technical elements report, therefore has certain novelty and novelty.
Summary of the invention
The invention aims to set up a set of Radix Psammosilenes and utilize band leaf tender stem cuttage quick breeding technology; thus break away from the single traditional planting models only relying on the cultivation of being enlarged of seminal propagation; the seedling utilizing cuttage technique to breed maintains the characteristic of female parent to greatest extent; a large amount of character can be obtained in a short time consistent simultaneously; the Radix Psammosilenes cutting seedling that growing way is vigorous; protection, group for wild Radix Psammosilenes resource recover and reasonably develop, thus a kind of method providing cottage propagation Radix Psammosilenes.
Solve the technical scheme that above-mentioned technical problem used to be made up of following step:
Step one, cutting are cultivated: band soil excavates wild or artificial culture Radix Psammosilenes, away from reed head 1-2cm, cut off all old branch and leaf, plant in nutrient bag, planting matrix sand red soil, water depending on soil moisture content, it is placed in ventilation on the sunny side, about 19-21d, is 3-8cm length and when not showing alabastrum until newly growing stolon, intercept with stainless steel blade and newly grow tender stem first stem above to blade section, as cutting material source；
Step 2, cutting whole piece is placed in the container filling with tap water immersion 30min, after suctioning water, with a stem Duan Weiyi group cuttage unit with a pair blade, cut off whole cutting, cuttage is on screen like iron wire network dish；Being placed in ferrum Dropbox to fill in the container of growth regulator and carry out immersion treatment, soak was not to have cutting base portion 0.5cm；
Step 3, through the immersion of 3-7d, when cutting incision initially forms callus, the related cutting of rolled steel wire will be soaked and totally take out, subsequently by cutting cuttage in the seedbed being covered with substrate, described substrate is suitable for cuttage rooting；During whole cuttage, humidity must not be less than 90%, and ventilation condition is good.
In described step 2, the growth regulator of selection is IBA, NAA and 2-4, D, concrete proportioning be IBA (0.1-2.0mg/L)+NAA (0.1-2.0mg/L)+
2-4, D (0.1-2.0mg/L).
In described step one, Radix Psammosilenes maternal plant pruning method is away from reed head 1-2cm, cuts off whole old branch and leaf and retains all branches base portion.
In described step one, Radix Psammosilenes cutting is new eruption and the tender stem not showing alabastrum.
In described step 2, Radix Psammosilenes is with a stem Duan Weiyi group cuttage unit with a pair blade, cuttage grid iron wire Dropbox.
In described step 2, Radix Psammosilenes cutting is placed in the container filling growth regulator immersion, and soak did not had cutting base portion 0.5cm.
In described step 3, Radix Psammosilenes cutting soaks 3-7d.
In described step 3, when cutting incision initially forms white or lilac callus, being taken out by cutting, cuttage is in the substrate that suitable Radix Psammosilenes is taken root.
Described Radix Psammosilenes maternal plant is the most suitable in spring, season in summer two.
The substrate of cuttage with fine sand, fertile soil, decomposed dung, concrete proportioning is: fine sand 10%-20%+ fertile soil 40%-50%+ decomposed dung 40%-50%.
Technique scheme is used to provide the benefit that:
Technical scheme, relative to prior seed seed propagation, has following benefit: reproduction speed is fast, only needs 3 months from outer implant to seedling；Breeding coefficient is high, and individual plant maternal plant once can about 30 parents of expanding propagation；It is affected by the external environment little, seedling the most single dependence seminal propagation；Seedling concordance is good, and the situation that separates occurs less；Small investment, high financial profit, can implantation in large scale; solve this bottleneck of source of seedling, utilize cutting seedling to carry out wild environment cultivation, not only contribute to the recovery of wild population; can contain that the predatoriness of wild resource is excavated simultaneously, produce good economy, society and ecological benefits.
Detailed description of the invention
Band soil excavates wild or artificial culture Radix Psammosilenes, away from reed head 1-2cm, cut off all old branch and leaf, plant in nutrient bag, planting matrix is preferred with sand red soil, water depending on soil moisture content, the plant growth regulator that plant establishment is had facilitation can be applied in right amount simultaneously, it is placed in ventilation on the sunny side, ambient temperature about 23 DEG C, after about one week, visible Radix Psammosilenes tender stem is from Lu Touchu eruption, growth time through about two weeks, stolon can grow to 3-8cm length, before buddingging, intercept with stainless steel blade and newly grow tender stem first stem above to blade section, as cutting material source；Radix Psammosilenes is taken root and the plant growth regulator of rudiment is IBA, NAA and 2-4, and D arranges in pairs or groups composition by a certain percentage；
It is placed in cutting whole piece to fill with and the container of tap water soaks 30min, after suctioning water, with a stem Duan Weiyi group cuttage unit with a pair blade, cutting off whole cutting, cuttage is on screen like iron wire network dish, and iron wire Dropbox selects quality harder, pressure, consistent being advisable of card flatness；When cutting is all paved with Dropbox, it is placed in Dropbox to fill in the container of plant growth regulator and carries out immersion treatment, soak is advisable not having cutting base portion 0.5cm, plant growth regulator is IBA, NAA and 2-4, D arranges in pairs or groups composition by a certain percentage, soak temperature in overall process and should not surpass 26 DEG C, in case soak is smelly or long mycete；
Immersion through 3-7d, incision can form a small amount of white or lilac callus, part cutting base portion there will be a little breach, now related for rolled steel wire cutting is taken out totally, with will cutting cuttage on the seedbed with substrate, described substrate is suitable for cuttage rooting, cutting medium is with river sand, fertile soil, well-rotted farmyard manure mixes by a certain percentage, after cuttage completes, keep seedbed zonal ventilation, shelter from heat or light, humidity is not less than 90%, after 15d, visible cutting base portion starts to sprout white tender hair of children, continue to be cultured to be a seedling, select carry out field-transplanting suitable season.
Radix Psammosilenes, after spring, season in summer two cut off old branches, easily sprouts tender stem, utilizes this characteristic of Radix Psammosilenes, uses new tender stem of sprouting to carry out cottage propagation, greatly improves speed and the efficiency of breeding, shorten finished product cultivation period, correspondingly reduce cultivation cost.
Present invention reduces the seedling time, use artificial controlled seedling in greenhouse to reduce the envirment factor impact on seedling；Utilize cottage propagation to carry out seedling extension to produce, it is to avoid because using seminal propagation, cause descendant inheritting trait segregation more, the drawback that quality discrepancy is bigger.
The above; being only the present invention preferably detailed description of the invention, but scope is not limited thereto, any those of ordinary skill in the art are in the technical scope that the invention discloses; the change that can make easily or replacement, within being included in the scope of protection of the invention.
Claims (5)
1. the method for a cottage propagation Radix Psammosilenes, it is characterised in that: it is made up of following step:
Step one, cutting are cultivated: band soil excavates wild or artificial culture Radix Psammosilenes, away from reed head 1-2cm, cut off all old branch and leaf, plant in nutrient bag, planting matrix sand red soil, water depending on soil moisture content, being placed in ventilation on the sunny side, 19-21d, is 3-8cm length and when not showing alabastrum until newly growing stolon, intercept with stainless steel blade and newly grow tender stem first stem above to blade section, as cutting material source；
Step 2, cutting whole piece is placed in the container filling with tap water immersion 30min, after suctioning water, with a stem Duan Weiyi group cuttage unit with a pair blade, cut off whole cutting, cuttage is on screen like iron wire network dish；Being placed in iron wire Dropbox to fill in the container of growth regulator and carry out immersion treatment, soak did not had cutting base portion 0.5cm；
Step 3, through the immersion of 3-7d, when cutting incision initially forms callus, the immersion related cutting of iron wire Dropbox is taken out totally, subsequently by cutting cuttage in the seedbed being covered with substrate, described substrate is suitable for cuttage rooting；During whole cuttage, humidity must not be less than 90%, and ventilation condition is good.
The method of cottage propagation Radix Psammosilenes the most according to claim 1, it is characterised in that: in described step 2, the growth regulator of selection is IBA, NAA and 2-4, D, and concrete proportioning is IBA0.1-2.0mg/L+NAA0.1-2.0mg/L+ 2-4, D
0.1-2.0mg/L。
The method of cottage propagation Radix Psammosilenes the most according to claim 1, it is characterised in that: in described step one, Radix Psammosilenes maternal plant pruning method is away from reed head 1-2cm, cuts off whole old branch and leaf and retains all branched base parts.
The method of cottage propagation Radix Psammosilenes the most according to claim 1, it is characterised in that: in described step 3, when cutting incision initially forms white or lilac callus, being taken out by cutting, cuttage is in the substrate that suitable Radix Psammosilenes is taken root.
The method of cottage propagation Radix Psammosilenes the most according to claim 1, it is characterised in that: the substrate of cuttage is fine sand, fertile soil, decomposed dung, and concrete proportioning is: fine sand 10%-20%+ fertile soil 40%-50%+ decomposed dung 40%-50%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410595186.XA CN104350918B (en) | 2014-10-30 | 2014-10-30 | A kind of method of cottage propagation Radix Psammosilenes |
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| CN201410595186.XA CN104350918B (en) | 2014-10-30 | 2014-10-30 | A kind of method of cottage propagation Radix Psammosilenes |
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| CN104350918A CN104350918A (en) | 2015-02-18 |
| CN104350918B true CN104350918B (en) | 2016-11-30 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284347B (en) * | 2015-10-14 | 2019-11-26 | 中国科学院昆明植物研究所 | Tuniclike psammosilene root fast-growing Technique on Cuttage Propagation |
| CN111316899B (en) * | 2020-03-23 | 2022-01-25 | 云南省农业科学院高山经济植物研究所 | Rapid rooting and seedling method for Psammosilene tunicoides through water culture |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101803521B (en) * | 2010-04-09 | 2011-07-06 | 云南省农业科学院高山经济植物研究所 | Greenhouse seedling raising method of Psammosilene tunicoides seed |
| CN103548513B (en) * | 2013-10-22 | 2015-09-16 | 香格里拉县富盛达生物科技有限责任公司 | A kind of tuniclike psammosilene root open-air rearing method |
| CN103766122A (en) * | 2014-01-24 | 2014-05-07 | 丽江益草生物资源开发有限责任公司 | Psammosilene tunicoides cultivation method |
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Granted publication date: 20161130 Termination date: 20191030 |