CN104326791A - Pleurotus nebrodensis culture matrix and culture method - Google Patents
Pleurotus nebrodensis culture matrix and culture method Download PDFInfo
- Publication number
- CN104326791A CN104326791A CN201310306900.4A CN201310306900A CN104326791A CN 104326791 A CN104326791 A CN 104326791A CN 201310306900 A CN201310306900 A CN 201310306900A CN 104326791 A CN104326791 A CN 104326791A
- Authority
- CN
- China
- Prior art keywords
- culture
- pleurotus nebrodensis
- culture material
- mycelia
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a pleurotus nebrodensis culture matrix and a culture method. The culture matrix comprises 40% of cottonseed shell, 20% of wood chip, 20% of cob, 13% of bran, 5% of corn flour, 1% of calcium superphosphate, and 1% of gypsum. The culture bag load is 450 g, the water content of 65% is beneficial for formation and differentiation of sporophore primordium, and the bacterial strain has the highest output and biological conversion rate when the pH of the culture material is 7.5. During strain culturing, when hypha grows over the culture material, 10 days of afterripening time is given, so that hypha can continue degrading the culture material, and sufficient accumulated nutrition is provided for growth of sporocarp. The synergic effect of the main materials is better than that of a single or two main materials, the nutritional compositions are relatively abundant and are relatively beneficial for absorption and utilization of hyphae, and employing of a liquid strain has the characteristics of fast seed propagation, low cost, inoculation convenience, short hypha growth time, consistent fungus age and tidy fruiting.
Description
Technical field
The present invention relates to fungus growing technique field, be specifically related to a kind of Pleurotus nebrodensis culture medium and cultivating method.
Background technology
Pleurotus nebrodensis formal name used at school Pleurotus nebrodensis [Pleurotus nebrodensis (Inzengae) Quel], the mutation of another name pleurotus eryngii white, Pleurotus eryngii var. nebrodensis, white glossy ganoderma mushroom and beautiful snow asafoetida mushroom etc., Basidiomycota, Hymenomycetes, Agaricales, Pleurotaceae, pleurotus.Pleurotus nebrodensis sporophore Dan Sheng, all living creatures or closely grow thickly, delicacy of bacterial context white, nondiscoloration, thick, slowly, organize fine and closely woven solid, shelf-lives is long, and quality is tender and crisp, unique flavor, commercially enjoys the title of " oyster mushroom king ", " plain abalone " in parachute-opening.Pleurotus nebrodensis fine and tender taste, delicious good to eat, there is higher edibleness, be described as " bolete on grassland " and pick up the ears, quite by the favor of human consumer.Pleurotus ferulae nutritious abundant, measure according to science, its protein content accounts for 20% of dry mushroom, containing 17 seed amino acids, and multivitamin and inorganic salt.Pleurotus nebrodensis also has certain pharmaceutical value, has disappear long-pending, desinsection, antibechic, the effect such as anti-inflammatory and control gynecological tumor.The pharmaceutical use of Pleurotus nebrodensis is very high, and it contains the physiologically active substance such as fungus polysaccharide and VITAMIN and several mineral materials, has and regulates human body physiological equilibrium, strengthens the effect of immune function of human body.At present; China's Cultivation of Pleurotus nebrodensis is based on traditional cultivation; it is very large by natural condition and the impact in season, and product can not whole year production, stable market supply; output is unstable; quality is uneven, and Pleurotus nebrodensis factory culture adopts industrialized technique means, under relatively controlled environment (temperature, light, gas, wet) facility condition; the mechanize of organizationally efficient rate, automated job, realize mass-producing, intensive, intelligent, stdn, anniversaryization produce.White clever No. 1 bacterial strain that the author selects Shanxi Ding Chang agricultural science and technology company limited to introduce carries out batch production bag and plants production, compares the impact of various processes on Pleurotus nebrodensis seed output and quality.
Pleurotus nebrodensis is a kind of edible Rare edible fungus all very high with pharmaceutical use.Its mushroom body colour pool is pure white, meat is fine and smooth, delicious flavour, rich in proteins 14.7%, fat 4.31%, robust fibre 15.4%, total amino acid content is 10.6%, and contains the mineral substance of multiple beneficial health, particularly fungus polysaccharide, has enhancing body immunity, regulates the effect of human body physiological equilibrium.
Summary of the invention
The object of the invention is to adopt Pleurotus nebrodensis (Pleurotus nebrodensis) bacterial strain to carry out batch production bag and plant test.Improve Cultivation of Pleurotus nebrodensis packed doses, the water content of the substratum self that grows of Pleurotus nebrodensis sporophore is improved, guarantee that its small rice grain bacterial classification has that mycelium germination speed is fast, mycelia is strong, inoculate feature easily, can shorten growth cycle, cost-saving; Promote that ripening time is on the impact of the growth of Pleurotus nebrodensis mycelia and mushroom flower bud divergaence time, seed output and quality.
To achieve these goals, the present invention adopts following technical scheme to realize:
One, Pleurotus nebrodensis culture medium composition of raw materials per-cent:
Cotton seed hulls 35% ~ 45%, wood chip 15% ~ 20%, corn cob 15% ~ 20%, wheat bran 8% ~ 15%, Semen Maydis powder %3% ~ 6%, calcium superphosphate 0.8% ~ 1.5%, gypsum 0.8% ~ 1.5%;
Two, the cultivating method of Pleurotus nebrodensis
1, bacterium bag makes and inoculation method:
(1). adopt wide 17cm-20cm, the polypropylene of long 35cm-40cm or polyethylene plastic bag.
(2). added by certain formula by raw material and puddle evenly, the ratio of adding water is generally 1: 1.3.
(3). culture material is puddled evenly, packs and nearly weighs 0.3-0.5 kilogram according to every packed siccative.
(4). pack rear and the sterilizing of fashionable dress pot, maintain 70-90 minute at high pressure 126 DEG C of temperature, at normal pressure 100 DEG C of temperature, maintain 12-16 hour.
(5). after sterilizing, bacterium bag is cooled to room temperature.Inoculate at inoculation tank or transfer room aseptic technique code routinely, every bottle of cultivar inoculates 20 bags.
(6). move into culturing room after inoculation and cultivate mycelia.Require room temperature 22 DEG C-26 DEG C, ventilation lucifugal, 35-40 days mycelia can purseful.
2, mushroom period management method:
(1). move into after mycelia purseful in fruiting place, open two ends sack, put 5-10 layer according to outside temperature.
(2). increase ambient moisture.The method of spraying is taked to make mushroom field ambient moisture increase to 85-95%.
(3). keep mushroom field well-ventilated, illumination is based on scattered light.
(4). under the condition of 10 DEG C-15 DEG C, within 10-20 days, bacterium bag two ends can form the small mushroom bud of Pleurotus nebrodensis.
(5). after mushroom flower bud is formed, increase ventilation, maintain ambient relative humidity and reach about 90%, no more than 95%.
(6). the same bacterium bag of Pleurotus nebrodensis can adopt mushroom 2-3 stubble, and biological efficiency can reach 50%-80%.
Beneficial effect result of the present invention is to be better than one or both major ingredient as effect mutually between major ingredient, and nutritive ingredient is abundanter, is more conducive to mycelium and absorbs.The water content of 65% is conducive to formation and the differentiation of fruit body primordium, and output is high, quality better.PH is the important factor affecting cell permeability, enzymic activity and Metabolic activity, and bacterial strain is when culture material pH is 7.5, and output, biological conversion rate are the highest.Adopt liquid spawn, breeding is fast, and cost is low, and inoculation is convenient, sends out bacterium short, and cell age is consistent, and fruiting is neat.
Embodiment
Be described further in conjunction with certain pleurotus nebrodensis edible mushroom cultivation experiments base.
Example one, a kind of Pleurotus nebrodensis culture medium and cultivating method adopt following technical scheme:
(1), Pleurotus nebrodensis culture medium composition of raw materials per-cent:
Cotton seed hulls 35% ~ 45%, wood chip 15% ~ 20%, corn cob 15% ~ 20%, wheat bran 8% ~ 15%, Semen Maydis powder %3% ~ 6%, calcium superphosphate 0.8% ~ 1.5%, gypsum 0.8% ~ 1.5%;
(2), the cultivating method of Pleurotus nebrodensis
1, bacterium bag makes and inoculation method:
(1). adopt wide 17cm-20cm, the polypropylene of long 35cm-40cm or polyethylene plastic bag.
(2). added by certain formula by raw material and puddle evenly, the ratio of adding water is generally 1: 1.3.
(3). culture material is puddled evenly, packs and nearly weighs 0.3-0.5 kilogram according to every packed siccative.
(4). pack rear and the sterilizing of fashionable dress pot, maintain 70-90 minute at high pressure 126 DEG C of temperature, at normal pressure 100 DEG C of temperature, maintain 12-16 hour.
(5). after sterilizing, bacterium bag is cooled to room temperature.Inoculate at inoculation tank or transfer room aseptic technique code routinely, every bottle of cultivar inoculates 20 bags.
(6). move into culturing room after inoculation and cultivate mycelia.Require room temperature 22 DEG C-26 DEG C, ventilation lucifugal, 35-40 days mycelia can purseful.
2, mushroom period management method:
(1). move into after mycelia purseful in fruiting place, open two ends sack, put 5-10 layer according to outside temperature.
(2). increase ambient moisture.The method of spraying is taked to make mushroom field ambient moisture increase to 85-95%.
(3). keep mushroom field well-ventilated, illumination is based on scattered light.
(4). under the condition of 10 DEG C-15 DEG C, within 10-20 days, bacterium bag two ends can form the small mushroom bud of Pleurotus nebrodensis.
(5). after mushroom flower bud is formed, increase ventilation, maintain ambient relative humidity and reach about 90%, no more than 95%.
(6). the same bacterium bag of Pleurotus nebrodensis can adopt mushroom 2-3 stubble, and biological efficiency can reach 50%-80%.
(3), the cultivation experiments of Pleurotus nebrodensis
1, Pleurotus nebrodensis output increases along with the increase of culture material charging quality, and culture material siccative quality is that the output of 500 g is the highest, but bacteria developing period is the longest, and biological conversion rate is minimum; Culture material siccative quality 450 g commodity mushroom proportion is maximum, and output is taken second place; Culture material siccative quality 350 g, bacteria developing period is the shortest, and output is minimum, and biological conversion rate is the highest.
2, Compost moisture content is on the impact of Pleurotus nebrodensis output and biological conversion rate
65% the bacterium time of Compost moisture content is placed in the middle, and output is the highest, and on average every bag of output is up to 213.72 g; Compost moisture content 67% output is taken second place, and bacteria developing period is the longest, and Compost moisture content 63% output, biological conversion rate are minimum, and bacteria developing period is the shortest.
3, culture material pH is on the impact of Pleurotus nebrodensis output and biological conversion rate
In 3 process, Pleurotus nebrodensis every bag fruiting number is suitable, and process 3 on average every bag of output is up to 221.06 g, and process 2 is taken second place, and on average every bag of output is minimum in process 1.Because culture material pH can reduce after sterilizing, and mycelia also can produce organic acid culture material pH is declined further in metabolic processes, thus affects output.Therefore, during cultivation Pleurotus nebrodensis spice, pH should be transferred to 7.5.
4, different cultivar is on the impact of Pleurotus nebrodensis growth cycle and output
4 kinds of cultivars are little to Pleurotus nebrodensis yield effect, but cultivar selects liquid spawn mycelium growth vigor strong, and color and luster is pure white, and growth cycle is the shortest; Select small rice grain bacterial classification mycelium growth vigor strong, color and luster is pure white, and growth cycle takes second place; Select cotton seed hulls bacterial classification mycelium growth vigor general, color and luster is pure white, and growth cycle is longer, and select waddy (Ice cream stick) bacterial classification mycelium growth vigor strong, color and luster is pure white, but growth cycle is longer.
5, bacterium bag different latter stage of ripening, is on the impact of Pleurotus nebrodensis seed output and quality
In spawn culture process, after mycelia covers with culture material, give the ripening time of 10 d, allow mycelia continue to degrade to culture material, accumulating enough nutritive substances provides needed for fruit body development.Cultivation of Pleurotus nebrodensis bag output and commodity mushroom proportion the highest, latter stage of ripening is 20 d, buddings fast, but yields poorly, if without latter stage of ripening, budding late, output is lower.
Conclusion: cultivation Pleurotus nebrodensis output and cultivation period are index, filter out optimum culture material formula, i.e. cotton seed hulls 40%, wood chip 20%, corn cob 20%, wheat bran 13%, Semen Maydis powder 5%, calcium superphosphate 1%, gypsum 1%.Be better than one or both major ingredient as effect mutually between 3 kinds of major ingredients, nutritive ingredient is abundanter, is more conducive to mycelium and absorbs.Factory culture Pleurotus nebrodensis adopts modern industry facility, equipment, and production cost increases greatly.Cultivating bag charging suitably increases, and output improves relatively, and when cultivating bag charge amount is 450 g, Pleurotus nebrodensis output is high, quality better.The water content of 65% is conducive to formation and the differentiation of fruit body primordium, and output is high, quality better.PH is the important factor affecting cell permeability, enzymic activity and Metabolic activity, and bacterial strain is when culture material pH is 7.5, and output, biological conversion rate are the highest.
Claims (4)
1. a Pleurotus nebrodensis culture medium and its culture medium formula of cultivating method are, cotton seed hulls 40%, wood chip 20%, corn cob 20%, wheat bran 13%, Semen Maydis powder 5%, calcium superphosphate 1%, gypsum 1%.
2. Pleurotus nebrodensis culture medium and its cultivating bag charge amount of cultivating method are 450 g, and the water content of 65% is conducive to formation and the differentiation of fruit body primordium, and bacterial strain is when culture material pH is 7.5, and output, biological conversion rate are the highest.
3. a Pleurotus nebrodensis culture medium and its bacterium bag of cultivating method make and with inoculation method are:
(1). adopt wide 17cm-20cm, the polypropylene of long 35cm-40cm or polyethylene plastic bag;
(2). added by certain formula by raw material and puddle evenly, the ratio of adding water is generally 1: 1.3;
(3). culture material is puddled evenly, packs and nearly weighs 0.3-0.5 kilogram according to every packed siccative;
(4). pack rear and the sterilizing of fashionable dress pot, maintain 70-90 minute at high pressure 126 DEG C of temperature, at normal pressure 100 DEG C of temperature, maintain 12-16 hour;
(5). after sterilizing, bacterium bag is cooled to room temperature, inoculates at inoculation tank or transfer room aseptic technique code routinely, every bottle of cultivar inoculates 20 bags;
(6). move into culturing room after inoculation and cultivate mycelia, require room temperature 22 DEG C-26 DEG C, ventilation lucifugal, 35-40 days mycelia can purseful.
4. as claimed in claim 3 a kind of Pleurotus nebrodensis culture medium and cultivating method its in spawn culture process, after mycelia covers with culture material, give the ripening time of 10 d, allow mycelia continue to degrade to culture material, accumulating enough nutritive substances provides needed for fruit body development.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310306900.4A CN104326791A (en) | 2013-07-22 | 2013-07-22 | Pleurotus nebrodensis culture matrix and culture method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310306900.4A CN104326791A (en) | 2013-07-22 | 2013-07-22 | Pleurotus nebrodensis culture matrix and culture method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104326791A true CN104326791A (en) | 2015-02-04 |
Family
ID=52401618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310306900.4A Pending CN104326791A (en) | 2013-07-22 | 2013-07-22 | Pleurotus nebrodensis culture matrix and culture method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104326791A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823703A (en) * | 2015-04-21 | 2015-08-12 | 吴中区胥口精益生物医药研究所 | Culture method for pleurotus nebrodensis |
| CN105399471A (en) * | 2015-11-30 | 2016-03-16 | 陶超杰 | Pleurotus nebrodensis nutrition bag and preparation method thereof |
| CN107434690A (en) * | 2016-05-26 | 2017-12-05 | 桂林市粮铭农业科技开发有限公司 | Application of the corncob mulberry tree branch in Pleurotus nebrodensis compost is made |
-
2013
- 2013-07-22 CN CN201310306900.4A patent/CN104326791A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823703A (en) * | 2015-04-21 | 2015-08-12 | 吴中区胥口精益生物医药研究所 | Culture method for pleurotus nebrodensis |
| CN105399471A (en) * | 2015-11-30 | 2016-03-16 | 陶超杰 | Pleurotus nebrodensis nutrition bag and preparation method thereof |
| CN107434690A (en) * | 2016-05-26 | 2017-12-05 | 桂林市粮铭农业科技开发有限公司 | Application of the corncob mulberry tree branch in Pleurotus nebrodensis compost is made |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101628834B (en) | Formula of toadstool culturing materials and toadstool natural culturing method | |
| CN102845219B (en) | Cultivation technique of Pleurotus eryngii | |
| CN101723759A (en) | Black fungus cultivation method and cultivation material thereof | |
| CN102630485B (en) | Method for cultivating flammulina velutipes through taking water-retaining agent solidified nutrient solution as culture medium | |
| CN103598015A (en) | Method for using liquid strains to cultivate agrocybe cylindracea | |
| CN107548989B (en) | Quinoa floating seedling raising method | |
| CN104987156B (en) | A kind of method of binwang mushroom culture medium and cultivation binwang mushroom using mushroom bran | |
| CN104557270A (en) | Black fungus culture method and culture medium suitable for northeast region | |
| CN102224792B (en) | Aquaculture method of selenium-rich hericium erinaceus | |
| CN102835252B (en) | Culture method of clitocybe maxima | |
| CN104303830A (en) | Planting method of Pleurotus eryngii | |
| CN110393126A (en) | A kind of method that industrial bottle plants elegant precious mushroom | |
| CN103444439A (en) | Earthing cultivation method of jinbian mushrooms | |
| CN104488545A (en) | Method for cultivating pleurotus nebrodensis, fresh edible fungi and coprinus comatus by virtue of cyclic utilization of straws | |
| CN109511461A (en) | A kind of high yield cultivating in bag method of black fungus | |
| CN102726210B (en) | Method for preparing cultivars of artificially-domesticated red-soil termitomyces albuminosus | |
| CN104012303A (en) | Cultivating method of pleurotus geesteranus edible mushroom | |
| CN103864537A (en) | Preparation method for morchella formula | |
| CN104641944A (en) | Method for culturing cordyceps sinensis by taking unhulled wheat as culture medium | |
| CN103975763A (en) | Agrocybe cylindracea cultivation method | |
| CN104326791A (en) | Pleurotus nebrodensis culture matrix and culture method | |
| CN103563652A (en) | Method for cultivating pleurotus cornucopiae at high yield and high efficiency in autumn and winter | |
| CN105493898A (en) | Culture method of needle mushrooms | |
| CN113748924A (en) | High-yield flower mushroom culture medium and preparation method and application thereof | |
| CN107873392A (en) | A kind of potted plant three-dimensional Industry Cultivation integration system of elegant precious mushroom and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150204 |