CN104288500A - Medicine for treating hemorrhagic conjunctivitis - Google Patents

Abstract

The invention provides a medicine for treating hemorrhagic conjunctivitis. The medicine is prepared from the following raw materials: concha haliotidis, golden cypress, honeysuckle, Chinese pulsatilla roots, prunella vulgaris, mint, gordon euryale seeds, cornus officinalis, lesser chickweed, caudate sweetleaves, indigofera tinctoria, radix rubiae, mimosa pudica, elephantopus scaber, polygonatum sibiricum, radix paeoniae alba and glycyrrhiza uralensis. According to the invention, the medicine is adopted, and ultrasonic atomization is adopted in combination with eye drops, so that the effect in treating the hemorrhagic conjunctivitis is significant, the course of disease is short, the times for long-term and partial eye dropping is reduced, stimulation can be prevented, the price is low, and adverse effects and drug resistance can be avoided.

Description

Be used for the treatment of the medicine of hemorrhagic conjunctivitis

Technical field

The present invention relates to technical field of Chinese medicine, particularly relate to a kind of medicine being used for the treatment of hemorrhagic conjunctivitis.

Background technology

Epidemic hemorrhagic conjunctivitis is that a kind of infectiousness is extremely strong, the acute conjunctivitis of outbreak of epidemic, and it is caused by rna viral infection.It is Inhibition Tests with Enterovirus Type 70 that World Health Organization (WHO) (WHO) has been infected viral definite designation, is listed in the third type infectious disease in China.

This disease is short for incubation period, natural history about 7 ~ 10 days, and severe one can reach 2 weeks or the longer time.Epidemic hemorrhagic conjunctivitis is mainly through contact transmission, and as shared towel, handkerchief, bath towel etc., summer and autumn is epidemic season, and wherein 8 ~ JIUYUE is epidemic peak.Its feature, for morbidity is anxious, infectiousness is strong, irritation weight, merges subconjunctival hemorrhage, corneal injury and preauricular lymph nodes enlargement more, can cause popular widely.Western medical treatment is antiviral eye liquid eye dripping mainly, but clinically commonly produces effects slow, and the course of disease is delayed.

Hemorrhagic conjunctivitis belongs to Chinese medicine epidemic febrile disease category, and claim acute and chronic conjunctivitis, epidemic keratoconjunctivitis, being commonly called as pink eye disease, is owing to experiencing ailment said due to cold or exposure pyretic toxicity, and invasion and attack human body eye causes.Acute and chronic conjunctivitis belongs to white of the eye disease, and white of the eye belongs to turbine, at dirty genus lung.Primary disease is many to be entered from table because epidemic disease poison is evil, and invade and lung meridian, wind heat is fought mutually, friendship is attacked caused by the order, and the lung and the large intestine being interior-exteriorly related, if heat accumulating in the large intestine, FU QI being obstructed, adverse flow of turbid QI, affects impairment of dispersing and descending function of the lung, sees hyperemia of the bulbar conjunctiva, chemosis, therefore wind and heat in the lung meridian is first should removing heat by catharsis expells the pathogenic heat, to get the meaning of removing burning wood away under the boiler, in treatment with heat-clearing and toxic substances removing improving eyesight, gently declare expelling pathogenic factors from the exterior, move back red detumescence for Therapeutic Principle.

The present invention is on the basis of theory of Chinese medical science, a kind of medicine being used for the treatment of hemorrhagic conjunctivitis is provided, the therapeutic modality of eye drop is coordinated by ultrasonic atomizatio, evident in efficacy in treatment hemorrhagic conjunctivitis, shorten the course of disease, reduce long-term Local eye drop number of times, prevent from causing stimulation, cheap, have no adverse reaction and drug resistance.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of medicine being used for the treatment of hemorrhagic conjunctivitis, the therapeutic modality of eye drop is coordinated by ultrasonic atomizatio, evident in efficacy in treatment hemorrhagic conjunctivitis, shorten the course of disease, reduce long-term Local eye drop number of times, prevent from causing stimulation, cheap, have no adverse reaction and drug resistance.

For solving the problems of the technologies described above, the invention provides a kind of medicine being used for the treatment of epidemic hemorrhagic conjunctivitis, its crude drug comprises: Concha Haliotidis, Cortex Phellodendri, Flos Lonicerae, the Radix Pulsatillae, Spica Prunellae, Herba Menthae, Semen Euryales, Fructus Corni, Herba Stellariae Alsines, Folium Symplocoris Caudatae, Folium et Caulis Indigoferae Tinctoriae, Radix Rubiae, Herba Mimosae Pudicae, Flos Trollii, Elephantopus scaber L., Rhizoma Polygonati, the Radix Paeoniae Alba and Radix Glycyrrhizae.

Wherein, in described medicine, the weight of each crude drug is respectively Concha Haliotidis 25g ~ 35g, Cortex Phellodendri 45g ~ 55g, Flos Lonicerae 35g ~ 45g, Radix Pulsatillae 35g ~ 45g, Spica Prunellae 15g ~ 25g, Herba Menthae 15g ~ 25g, Semen Euryales 20g ~ 30g, Fructus Corni 25g ~ 35g, Herba Stellariae Alsines 10g ~ 20g, Folium Symplocoris Caudatae 15g ~ 25g, Folium et Caulis Indigoferae Tinctoriae 15g ~ 25g, Radix Rubiae 25g ~ 35g, Herba Mimosae Pudicae 35g ~ 45g, Flos Trollii 30g ~ 40g, Elephantopus scaber L. 25g ~ 35g, Rhizoma Polygonati 30g ~ 40g, Radix Paeoniae Alba 15g ~ 25g and Radix Glycyrrhizae 20g ~ 30g.

Wherein, in described medicine, the weight of each crude drug is respectively Concha Haliotidis 28 ~ 32g, Cortex Phellodendri 48g ~ 52g, Flos Lonicerae 38g ~ 42g, Radix Pulsatillae 38g ~ 42g, Spica Prunellae 18g ~ 22g, Herba Menthae 18g ~ 22g, Semen Euryales 23g ~ 27g, Fructus Corni 28g ~ 32g, Herba Stellariae Alsines 13g ~ 17g, Folium Symplocoris Caudatae 18g ~ 22g, Folium et Caulis Indigoferae Tinctoriae 18g ~ 22g, Radix Rubiae 28g ~ 32g, Herba Mimosae Pudicae 38g ~ 42g, Flos Trollii 33g ~ 37g, Elephantopus scaber L. 28g ~ 32g, Rhizoma Polygonati 33g ~ 37g, Radix Paeoniae Alba 18g ~ 22g and Radix Glycyrrhizae 23g ~ 27g.

Wherein, in described medicine, the weight of each crude drug is respectively Concha Haliotidis 30g, Cortex Phellodendri 50g, Flos Lonicerae 40g, Radix Pulsatillae 40g, Spica Prunellae 20g, Herba Menthae 20g, Semen Euryales 25g, Fructus Corni 30g, Herba Stellariae Alsines 15g, Folium Symplocoris Caudatae 20g, Folium et Caulis Indigoferae Tinctoriae 20g, Radix Rubiae 30g, Herba Mimosae Pudicae 40g, Flos Trollii 35g, Elephantopus scaber L. 30g, Rhizoma Polygonati 35g, Radix Paeoniae Alba 20g and Radix Glycyrrhizae 25g.

Wherein, described medicine can be prepared into decoction liquor and eye drop.

Present invention also offers the preparation method of said medicine, when prepared medicine is decoction liquor, be specially: each crude drug component is pulverized respectively, put into blender to be uniformly mixed, obtain mixture, add the water relative to mixture quality 2 ~ 4 times, decoct 3 ~ 5 hours, decoction liquor is filtered, filtering residue adds the water relative to the mixture quality prepared 2 ~ 4 times again above, decocts 2 ~ 4 hours, and decoction liquor is filtered, twice decoction liquor is merged, obtains decoction liquor.

Present invention also offers the preparation method of said medicine, when prepared medicine is eye drop, be specially:

The first step, by Concha Haliotidis, Cortex Phellodendri, Flos Lonicerae, the Radix Pulsatillae, Radix Rubiae, Herba Mimosae Pudicae, Flos Trollii, Elephantopus scaber L., Rhizoma Polygonati, the Radix Paeoniae Alba and Radix Glycyrrhizae are by above-mentioned weight mixing, with the alcohol reflux 2 times ~ 4 times that determining alcohol is 80% ~ 90%, the amount of the ethanol added is the mixture quality 4 ~ 6 times that this step obtains, filter, merging filtrate, be evaporated to, when 60 DEG C, relative density is the mastic of 1.15 ~ 1.20, add the water dissolution relative to mastic quality 3 ~ 5 times amount subsequently, add relative to mass percentage concentration 5% ~ 10% sodium hydrate aqueous solution hydrotropy under stirring, adjust ph is 7 ~ 8, filter, add concentration be 10% salt acid for adjusting pH value be 3 ~ 4, static 2 ~ 4 hours, filter, filtrate reduced in volume obtains and gets dry extract, be ground into 50 order ~ 100 object powder,

Second step, by remaining components by above-mentioned weight mixing, obtain mixture, add the water relative to mixture quality 2 ~ 4 times, decoct 3 ~ 5 hours, decoction liquor is filtered, filtering residue adds the water relative to the mixture quality prepared 2 ~ 4 times again above, decoct 2 ~ 4 hours, decoction liquor is filtered, twice decoction liquor is merged, add concentration be 10% salt acid for adjusting pH value be 3 ~ 4, static 2 ~ 4 hours, filter, filtrate alcohol extraction, extract concentrating under reduced pressure becomes dry mastic, dry subsequently, be ground into 50 order ~ 100 object powder, the powder that this powder and the first step obtain is mixed, obtain fine powder,

3rd step, by the sodium chloride of 1g ~ 2g, 0.5g ~ 1g mannitol, 0.03g ~ 0.06g hydroxy benzenes ethyl ester, add in appropriate sterilized water for injection, stir, add the fine powder that second step obtains subsequently, stirring and dissolving is complete, stir evenly, continue to add sterilized water for injection, to the crude drug concentration of eye drop is 1g/ml, after stirring, with filtering with microporous membrane, at 100 DEG C, use flowing steam sterilization 30min, subpackage under aseptic condition.

Wherein, it is carrier that the using method of described decoction liquor is specially with ultrasound atomizer, by in the prepared decoction liquor nebulizer container of falling people, connect plastic nozzle by mist pipe, nozzle is just to trouble eye, and patient opens eyelid and accepts treatment, make medicinal liquid form atomization molecule by supersonic vibration and arrive bulbar conjunctiva and cornea, distance is 20cm, and start timing 20 minutes, is atomized the clean napkin of complete use and dries.

Beneficial effect of the present invention:

The medicine being used for the treatment of hemorrhagic conjunctivitis provided by the invention, coordinates the therapeutic modality of eye drop by ultrasonic atomizatio, evident in efficacy in treatment hemorrhagic conjunctivitis, shorten the course of disease, reduce long-term Local eye drop number of times, prevent from causing stimulation, cheap, have no adverse reaction and drug resistance.

Detailed description of the invention

The invention provides a kind of medicine being used for the treatment of epidemic hemorrhagic conjunctivitis, its crude drug comprises: Concha Haliotidis, Cortex Phellodendri, Flos Lonicerae, the Radix Pulsatillae, Spica Prunellae, Herba Menthae, Semen Euryales, Fructus Corni, Herba Stellariae Alsines, Folium Symplocoris Caudatae, Folium et Caulis Indigoferae Tinctoriae, Radix Rubiae, Herba Mimosae Pudicae, Flos Trollii, Elephantopus scaber L., Rhizoma Polygonati, the Radix Paeoniae Alba and Radix Glycyrrhizae.

Further, described medicine is only made up of above-mentioned raw materials medicine.

In described medicine, the weight of each crude drug is respectively Concha Haliotidis 25g ~ 35g, Cortex Phellodendri 45g ~ 55g, Flos Lonicerae 35g ~ 45g, Radix Pulsatillae 35g ~ 45g, Spica Prunellae 15g ~ 25g, Herba Menthae 15g ~ 25g, Semen Euryales 20g ~ 30g, Fructus Corni 25g ~ 35g, Herba Stellariae Alsines 10g ~ 20g, Folium Symplocoris Caudatae 15g ~ 25g, Folium et Caulis Indigoferae Tinctoriae 15g ~ 25g, Radix Rubiae 25g ~ 35g, Herba Mimosae Pudicae 35g ~ 45g, Flos Trollii 30g ~ 40g, Elephantopus scaber L. 25g ~ 35g, Rhizoma Polygonati 30g ~ 40g, Radix Paeoniae Alba 15g ~ 25g and Radix Glycyrrhizae 20g ~ 30g.

Further, in described medicine, the weight of each crude drug is respectively Concha Haliotidis 28 ~ 32g, Cortex Phellodendri 48g ~ 52g, Flos Lonicerae 38g ~ 42g, Radix Pulsatillae 38g ~ 42g, Spica Prunellae 18g ~ 22g, Herba Menthae 18g ~ 22g, Semen Euryales 23g ~ 27g, Fructus Corni 28g ~ 32g, Herba Stellariae Alsines 13g ~ 17g, Folium Symplocoris Caudatae 18g ~ 22g, Folium et Caulis Indigoferae Tinctoriae 18g ~ 22g, Radix Rubiae 28g ~ 32g, Herba Mimosae Pudicae 38g ~ 42g, Flos Trollii 33g ~ 37g, Elephantopus scaber L. 28g ~ 32g, Rhizoma Polygonati 33g ~ 37g, Radix Paeoniae Alba 18g ~ 22g and Radix Glycyrrhizae 23g ~ 27g.

Most preferably, in described medicine, the weight of each crude drug is respectively Concha Haliotidis 30g, Cortex Phellodendri 50g, Flos Lonicerae 40g, Radix Pulsatillae 40g, Spica Prunellae 20g, Herba Menthae 20g, Semen Euryales 25g, Fructus Corni 30g, Herba Stellariae Alsines 15g, Folium Symplocoris Caudatae 20g, Folium et Caulis Indigoferae Tinctoriae 20g, Radix Rubiae 30g, Herba Mimosae Pudicae 40g, Flos Trollii 35g, Elephantopus scaber L. 30g, Rhizoma Polygonati 35g, Radix Paeoniae Alba 20g and Radix Glycyrrhizae 25g.

Described medicine is prepared into eye drop and decoction liquor, and decoction liquor is treated patient by ultrasonic atomizatio.

Present invention also offers the preparation method of said medicine, when prepared medicine is decoction liquor, be specially: each crude drug component is pulverized respectively, put into blender to be uniformly mixed, obtain mixture, add the water relative to mixture quality 2 ~ 4 times, decoct 3 ~ 5 hours, decoction liquor is filtered, filtering residue adds the water relative to the mixture quality prepared 2 ~ 4 times again above, decocts 2 ~ 4 hours, and decoction liquor is filtered, twice decoction liquor is merged, obtains decoction liquor.

Present invention also offers the preparation method of said medicine, when prepared medicine is eye drop, be specially:

The first step, by Concha Haliotidis, Cortex Phellodendri, Flos Lonicerae, the Radix Pulsatillae, Radix Rubiae, Herba Mimosae Pudicae, Flos Trollii, Elephantopus scaber L., Rhizoma Polygonati, the Radix Paeoniae Alba and Radix Glycyrrhizae are by above-mentioned weight mixing, with the alcohol reflux 2 times ~ 4 times that determining alcohol is 80% ~ 90%, the amount of the ethanol added is the mixture quality 4 ~ 6 times that this step obtains, filter, merging filtrate, be evaporated to, when 60 DEG C, relative density is the mastic of 1.15 ~ 1.20, add the water dissolution relative to mastic quality 3 ~ 5 times amount subsequently, add relative to mass percentage concentration 5% ~ 10% sodium hydrate aqueous solution hydrotropy under stirring, adjust ph is 7 ~ 8, filter, add concentration be 10% salt acid for adjusting pH value be 3 ~ 4, static 2 ~ 4 hours, filter, filtrate reduced in volume obtains and gets dry extract, be ground into 50 order ~ 100 object powder,

Second step, by remaining components by above-mentioned weight mixing, obtain mixture, add the water relative to mixture quality 2 ~ 4 times, decoct 3 ~ 5 hours, decoction liquor is filtered, filtering residue adds the water relative to the mixture quality prepared 2 ~ 4 times again above, decoct 2 ~ 4 hours, decoction liquor is filtered, twice decoction liquor is merged, add concentration be 10% salt acid for adjusting pH value be 3 ~ 4, static 2 ~ 4 hours, filter, filtrate alcohol extraction, extract concentrating under reduced pressure becomes dry mastic, dry subsequently, be ground into 50 order ~ 100 object powder, the powder that this powder and the first step obtain is mixed, obtain fine powder,

3rd step, by the sodium chloride of 1g ~ 2g, 0.5g ~ 1g mannitol, 0.03g ~ 0.06g hydroxy benzenes ethyl ester, add in appropriate sterilized water for injection, stir, add the fine powder that second step obtains subsequently, stirring and dissolving is complete, stir evenly, continue to add sterilized water for injection, to the crude drug concentration of eye drop is 1g/ml, after stirring, with filtering with microporous membrane, at 100 DEG C, use flowing steam sterilization 30min, subpackage under aseptic condition.

In treatment epidemic hemorrhagic conjunctivitis, during general conventional eye drip treatment, medicine is low at conjunctival sac distributed density, action time is not long, and absorbs slow, poor permeability.And current more eye drop is irritant, after eye drop enters conjunctival sac, lacrimal secretion is caused to increase, before medicine not yet enters tissue, very easily diluted by tear, affect pharmaceutically-active performance, but, it is advantageous that and can use at any time, for patient treatment is provided convenience.Ultrasonic atomizatio is that aerosol trickle for medicinal atomized one-tenth is acted on focus by using ultrasound ripple, at local reachability to higher effective treatment concentration and persistent period, nebulae inhalation can make medicine contact eye table better, also have hot-wet compress effect to promote the absorption of inflammation, fumigating by air-flow, can accelerate local blood circulation simultaneously, disperse exopathogen, pass unimpeded QI and blood, but its Therapeutic Method is comparatively complicated, not easily uses at any time.

During atomization, medical liquid atomizing becomes micro particles to directly act on affected part, can make medicinal liquid local reach valid density and and continue for some time, diagonal angle conjunctiva plays direct osmosis, is conducive to angle conjunctiva to drug absorption; 2. stifling, the impact of air-flow can make a local blood circulation increase, and have stronger suppression and killing action to pathogen; 3. during atomization, steam can dilute antigen in tear, in and pH of tears, can the symptom of very fast reduction of patient.

It is carrier that the using method of described decoction liquor is specially with ultrasound atomizer, by in the prepared decoction liquor nebulizer container of falling people, plastic nozzle is connected by mist pipe, nozzle is just to trouble eye, patient opens eyelid and accepts treatment, and make medicinal liquid form atomization molecule by supersonic vibration and arrive bulbar conjunctiva and cornea, distance is 20cm, start timing 20 minutes, is atomized the clean napkin of complete use and dries.

The pharmacology of each crude drug is as follows:

Concha Haliotidis: taste becomes, and property is put down, and has effect of suppressing the hyperactive liver to relieve the wind syndrome, YANG hyperactivity suppressing, heat extraction improving eyesight, cures mainly the diseases such as excessive rising of liver-YANG, the vertigo, asthenia hectic fever due to YIN-deficiency, haematemesis, optic atrophy.

Cortex Phellodendri: bitter in the mouth, cold in nature, enter kidney, bladder, large intestine channel, heat clearing and damp drying, eliminating fire and detoxication, except hectic fever due to YIN-deficiency clearind deficient heat.For damp-heat dysentery, jaundice, leukorrhagia, pyretic stranguria, beriberi, flaccidity is warded off, hectic fever due to YIN-deficiency consumptive fever, night sweat, seminal emission, sore swollen toxin, eczema pruritus.

Flos Lonicerae: have another name called Flos Lonicerae, Flos Lonicerae, Flos Lonicerae, two precious flowers, sweet in the mouth, cold in nature, enter the heart, lung, stomach, large intestine channel, there is the effect of heat-clearing and toxic substances removing, double energy eliminating pathogenic heat from blood to cure dysentery, is mainly used in affection due to external wind and heat, epidemic febrile disease pyretic toxicity is comparatively contained, or when epidemic febrile disease is still not descending at the part of the body cavity above the diaphragm housing the heart and lungs from the beginning of pathogenic heat; Pyretic toxicity dysentery, stool hematochezia; Fever disease in summer is demonstrate,proved; The carbuncle sore that pyretic toxicity gathers is from the beginning of the ， inflammation heat person of swelling and ache, and other skin infections, and this thing has good antipyretic effect, has antiinflammatory action, can promote leukocytic phagocytic function, have stronger bacteriostasis to multiple pyococcus and pathogenic entero becteria.

The Radix Pulsatillae: bitter in the mouth, cold in nature, enters stomach, large intestine channel, has heat-clearing and toxic substances removing, the effect of eliminating pathogenic heat from blood to cure dysentery, and being mainly used in gastrointestinal has that damp and hot stagnant heating is suffered from abdominal pain, dysentery pus and blood, the cards such as tenesmus; To the hematodiarrhoea hematochezia better effects if of large intestine heat in blood, there is stronger inhibitory action to dysentery bacterium, Bacillus typhi, bacillus subtilis, escherichia coli, Staphyloccocus aureus, Sharpe door bacterium.

Spica Prunellae: cold in nature, bitter in the mouth, pungent, return liver, gallbladder meridian, have the effect of liver heat removing and eyesight improving, powder detoxifcation, for conjunctival congestion and swelling pain, order pearl nyctalgia, has a headache dizzy, scrofula, and goiter, swells and ache; Thyromegaly, tuberculous lymphadenitis, cyclomastopathy, the cards such as hypertension, hyperglycemia, hyperlipidemia and high blood viscosity, have obvious inhibitory action to dysentery bacterium, cholera bacilli, Bacillus typhi, escherichia coli, Bacillus proteus, staphylococcus.

Herba Menthae: acrid in the mouth, cool in nature, enters lung, Liver Channel, has effect of dispelling wind and heat pathogens, the clear sharp head, sore-throat relieving rash, dispersing the stagnated live-QI to relieve the stagnation of QI, for affection due to external wind and heat or epidemic febrile disease from the beginning of, headache due to pathogenic wind-heat, the many tear of Ramulus et Folium Bischofiae Javanicae, laryngopharynx swelling and pain, measles is sent out not smooth thoroughly, and rubella is scratched where it itches, irritability stasis, and uncomfortable in chest not relaxing waits card.

Semen Euryales: sweet in the mouth, puckery, property is put down.Return spleen, kidney channel.Have supplementing the kidney to control the nocturnal, spleen reinforcing antidiarrheal, the function of damp eliminating leukorrhagia stopping, cures mainly emission, diarrhea due to hypofunction of the spleen, and insufficiency of the spleen nebulousurine is with inferior card.

Fructus Corni: latin name corni feuctus, sour in the mouth, puckery, slightly warm in nature, returns liver, kidney channel, has liver and kidney tonifying, effect of essence astringing and desertion stemming.For vertigo and tinnitus, soreness of waist and knee joint, impotence and seminal emission, enuresis frequent micturition, bleeding not during menses, profuse sweating is collapsed.Interior-heat such as to be quenched one's thirst at the card.

Herba Stellariae Alsines: be the herb of Caryophyllaceae Stellaria plant Stellaria alsine Grim., acrid in the mouth, property is put down, and returns lung, spleen channel, has expelling wind and removing dampness, promoting blood circulation and detumescence, and effect of removing toxic substances hemostasis, cures mainly flu, dysentery, rheumatic arthralgia, traumatic injury, venom, spits blood, the cards such as traumatic hemorrhage.

Folium Symplocoris Caudatae: be the leaf of vitriol platymiscium mountain, Symplocaceae mountain vitriol, sour in the mouth, puckery, micro-sweet, property is put down, and have heat-clearing and toxic substances removing, the therapeutic method to keep the adverse QI flowing downwards, effect of hemostasis, cures mainly chronic dysentery, wind-fire acute conjunctivitis, tonsillitis, otitis media, cough, vomiting, hemoptysis, the card such as to have blood in stool.

Folium et Caulis Indigoferae Tinctoriae: be the stem and leaf of pulse family anil Folium et Caulis Indigoferae Tinctoriae, mildly bitter flavor, cold in nature, have heat-clearing and toxic substances removing, effect of cooling blood for hemostasis, cures mainly encephalitis B, parotitis, acute pharyngolaryngitis, lymphadenitis, conjunctival congestion, aphtha, erysipelas, scabies, worm venom, the cards such as haematemesis.

Radix Rubiae: cold in nature, bitter in the mouth, returns Liver Channel, effect: cooling blood for hemostasis; Blood circulation promoting and blood stasis dispelling, cards such as curing mainly heat in blood spitting of blood, haematemesis, epistaxis, hematuria, have blood in stool, metrorrhagia, amenorrhea, postpartum stagnation stomachache, traumatic injury, rheumatic arthralgia, jaundice, carbuncle sore, hemorrhoid are swollen.

Herba Mimosae Pudicae: sweet in the mouth, puckery, micro-hardship, cold nature, returns spleen, kidney two warp, have removing pathogenic heat from blood and toxic substance from the body, clearing away heat-damp and promoting diuresis, effect of tranquillizing and allaying excitement, cures mainly flu, infantile hyperpyrexia, bronchitis, hepatitis, gastritis, conjunctivitis, urinary system calculus, edema, hemoptysis, hematuria, neurasthenia, insomnia, sore swollen toxin, herpes zoster, the cards such as traumatic injury.

Flos Trollii: latin name Trollius chinensis, bitter in the mouth, cool in nature, there is effect of heat-clearing and toxic substances removing, cure mainly acute and chronic tonsillitis, acute otitis media, acute myringitis, acute conjunctivitis, the cards such as acute lymphangitis.

Elephantopus scaber L.: cold in nature, bitter in the mouth is pungent, has heat-clearing and toxic substances removing, and effect of inducing diuresis to remove edema, cures mainly flu, acute tonsillitis, pharyngolaryngitis, eye conjunctivitis, epidemic encephalitis type B, pertussis, acute icterohepatitis, cirrhotic ascites, acute and chronic nephritis, furuncle and phyma, the cards such as eczema.

Rhizoma Polygonati: sweet in the mouth, property is put down, and enters lung, spleen, kidney channel, has nourishing kidney lung moistening, the effect of invigorating the spleen and replenishing QI, for deficiency of YIN chronic cough; Xeropulmonary cough; Insufficiency of the spleen weak; Lack of appetite xerostomia; Quench one's thirst; Deficiency of the kidney soreness of the waist and knees; Impotence and seminal emission; Tinnitus order is dark; Early whitening of beard and hair; Body void is won thin; The cards such as wind favus disease.

The Radix Paeoniae Alba: bitter in the mouth, acid, cold nature, enters liver, spleen channel, joint venture of nourishing blood, easing the affected liver to relieve pain, and the effect of suppressing liver-YANG is mainly used in deficiecny of liver-YIN, the headache feeling of fullness in the head of excessive rising of liver-YANG, vertigo and tinnitus, irritable; Deficiency of liver-blood, battalion defends the menoxenia of inanition, metrorrhagia and metrostaxis; The dysmenorrhea of deficiency of the liver stasis dampness; Disharmony of nutrient QI with defensive QI, the cloudy outer inducing vomiting spontaneous sweating of battalion; The gastral cavity abdomen pain over the hypochondriac region of depression of liver-QI, dysentery is suffered from abdominal pain; Deficiency and exhaustion of liver-YIN, passages through which vital energy circulates loses the cards such as the carpopedal spasm pain of supporting.

Radix Glycyrrhizae: sweet in the mouth, property put down, enter 12 warps, have heat-clearing and toxic substances removing, be in harmonious proportion the property of medicine, the effect of expelling phlegm for arresting cough, and can spleen invigorating and in, relieving spasm to stop pain, is mainly used in the abdominal distension and anorexia of weakness of the spleen and stomach, weak; Can alleviate contracture pain in abdomen, relax the strong and toxicity of some drugs, alleviating medicine stimulates to the toxic and side effects of body or to gastrointestinal.

Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.

Embodiment 1 decoction liquor 1

Concha Haliotidis 30g, Cortex Phellodendri 50g, Flos Lonicerae 40g, Radix Pulsatillae 40g, Spica Prunellae 20g, Herba Menthae 20g, Semen Euryales 25g, Fructus Corni 30g, Herba Stellariae Alsines 15g, Folium Symplocoris Caudatae 20g, Folium et Caulis Indigoferae Tinctoriae 20g, Radix Rubiae 30g, Herba Mimosae Pudicae 40g, Flos Trollii 35g, Elephantopus scaber L. 30g, Rhizoma Polygonati 35g, Radix Paeoniae Alba 20g and Radix Glycyrrhizae 25g are pulverized respectively, put into blender to be uniformly mixed, obtain mixture, add the water of 1.5kg, decoct 4 hours, decoction liquor is filtered, filtering residue adds the water of 1.5kg again, decoct 3 hours, decoction liquor is filtered, twice decoction liquor is merged, obtains decoction liquor 1.

Embodiment 2 decoction liquor 2

Concha Haliotidis 28g, Cortex Phellodendri 48g, Flos Lonicerae 38g, Radix Pulsatillae 38g, Spica Prunellae 18g, Herba Menthae 18g, Semen Euryales 23g, Fructus Corni 28g, Herba Stellariae Alsines 13g, Folium Symplocoris Caudatae 18g, Folium et Caulis Indigoferae Tinctoriae 18g, Radix Rubiae 28g, Herba Mimosae Pudicae 38g, Flos Trollii 33g, Elephantopus scaber L. 28g, Rhizoma Polygonati 33g, Radix Paeoniae Alba 18g and Radix Glycyrrhizae 23g are pulverized respectively, put into blender to be uniformly mixed, obtain mixture, add the water of 1.5kg, decoct 4 hours, decoction liquor is filtered, filtering residue adds the water of 1.5kg again, decoct 3 hours, decoction liquor is filtered, twice decoction liquor is merged, obtains decoction liquor 2.

Embodiment 3 decoction liquor 3

Concha Haliotidis 32g, Cortex Phellodendri 52g, Flos Lonicerae 42g, Radix Pulsatillae 42g, Spica Prunellae 22g, Herba Menthae 22g, Semen Euryales 27g, Fructus Corni 32g, Herba Stellariae Alsines 17g, Folium Symplocoris Caudatae 22g, Folium et Caulis Indigoferae Tinctoriae 22g, Radix Rubiae 32g, Herba Mimosae Pudicae 42g, Flos Trollii 37g, Elephantopus scaber L. 32g, Rhizoma Polygonati 37g, Radix Paeoniae Alba 22g and Radix Glycyrrhizae 27g are pulverized respectively, put into blender to be uniformly mixed, obtain mixture, add the water of 1.5kg, decoct 4 hours, decoction liquor is filtered, filtering residue adds the water of 1.5kg again, decoct 3 hours, decoction liquor is filtered, twice decoction liquor is merged, obtains decoction liquor 3.

Embodiment 4 eye drop 1

By Concha Haliotidis 30g, Cortex Phellodendri 50g, Flos Lonicerae 40g, Radix Pulsatillae 40g, Radix Rubiae 30g, Herba Mimosae Pudicae 40g, Flos Trollii 35g, Elephantopus scaber L. 30g, Rhizoma Polygonati 35g, Radix Paeoniae Alba 20g and Radix Glycyrrhizae 25g mixes, by the ethanol 1.8kg reflux, extract, 3 times that determining alcohol is 90%, filter, merging filtrate, be evaporated to, when 60 DEG C, relative density is the mastic of 1.20, add the water dissolution relative to mastic quality 4 times amount subsequently, add relative to mass percentage concentration 5% sodium hydrate aqueous solution hydrotropy under stirring, adjust ph is 7, filter, add concentration be 10% salt acid for adjusting pH value be 3, static 3 hours, filter, filtrate reduced in volume obtains and gets dry extract, be ground into 100 object powder, by Spica Prunellae 20g, Herba Menthae 20g, Semen Euryales 25g, Fructus Corni 30g, Herba Stellariae Alsines 15g, Folium Symplocoris Caudatae 20g, Folium et Caulis Indigoferae Tinctoriae 20g mixes, obtain mixture, add the water of 450g, decoct 4 hours, decoction liquor is filtered, filtering residue adds the water of 450g again, decoct 3 hours, decoction liquor is filtered, twice decoction liquor is merged, add concentration be 10% salt acid for adjusting pH value be 3, static 3 hours, filter, filtrate alcohol extraction, extract concentrating under reduced pressure becomes dry mastic, dry subsequently, be ground into 100 object powder, this powder is mixed with the powder obtained above, obtain fine powder, by the sodium chloride of 2g, 1g mannitol, 0.06g hydroxy benzenes ethyl ester, add in appropriate sterilized water for injection, stir, add the fine powder obtained subsequently above, stirring and dissolving is complete, stir evenly, continue to add sterilized water for injection, to the crude drug concentration of eye drop is 1g/ml, after stirring, with filtering with microporous membrane, flowing steam sterilization 30min is used at 100 DEG C, subpackage under aseptic condition, obtain eye drop 1.

Embodiment 5 eye drop 2

By Concha Haliotidis 28g, Cortex Phellodendri 48g, Flos Lonicerae 38g, Radix Pulsatillae 38g, Radix Rubiae 28g, Herba Mimosae Pudicae 38g, Flos Trollii 33g, Elephantopus scaber L. 28g, Rhizoma Polygonati 33g, Radix Paeoniae Alba 18g and Radix Glycyrrhizae 23g mixes, by the ethanol 1.8kg reflux, extract, 3 times that determining alcohol is 90%, filter, merging filtrate, be evaporated to, when 60 DEG C, relative density is the mastic of 1.20, add the water dissolution relative to mastic quality 4 times amount subsequently, add relative to mass percentage concentration 5% sodium hydrate aqueous solution hydrotropy under stirring, adjust ph is 7, filter, add concentration be 10% salt acid for adjusting pH value be 3, static 3 hours, filter, filtrate reduced in volume obtains and gets dry extract, be ground into 100 object powder, by Spica Prunellae 18g, Herba Menthae 18g, Semen Euryales 23g, Fructus Corni 28g, Herba Stellariae Alsines 13g, Folium Symplocoris Caudatae 18g, Folium et Caulis Indigoferae Tinctoriae 18g mixes, obtain mixture, add the water of 450g, decoct 4 hours, decoction liquor is filtered, filtering residue adds the water of 450g again, decoct 3 hours, decoction liquor is filtered, twice decoction liquor is merged, add concentration be 10% salt acid for adjusting pH value be 3, static 3 hours, filter, filtrate alcohol extraction, extract concentrating under reduced pressure becomes dry mastic, dry subsequently, be ground into 100 object powder, this powder is mixed with the powder obtained above, obtain fine powder, by the sodium chloride of 2g, 1g mannitol, 0.06g hydroxy benzenes ethyl ester, add in appropriate sterilized water for injection, stir, add the fine powder obtained subsequently above, stirring and dissolving is complete, stir evenly, continue to add sterilized water for injection, to the crude drug concentration of eye drop is 1g/ml, after stirring, with filtering with microporous membrane, flowing steam sterilization 30min is used at 100 DEG C, subpackage under aseptic condition, obtain eye drop 2.

Embodiment 6 eye drop 3

By Concha Haliotidis 32g, Cortex Phellodendri 52g, Flos Lonicerae 42g, Radix Pulsatillae 42g, Radix Rubiae 32g, Herba Mimosae Pudicae 42g, Flos Trollii 37g, Elephantopus scaber L. 32g, Rhizoma Polygonati 37g, Radix Paeoniae Alba 22g and Radix Glycyrrhizae 27g mixes, by the ethanol 1.8kg reflux, extract, 3 times that determining alcohol is 90%, filter, merging filtrate, be evaporated to, when 60 DEG C, relative density is the mastic of 1.20, add the water dissolution relative to mastic quality 4 times amount subsequently, add relative to mass percentage concentration 5% sodium hydrate aqueous solution hydrotropy under stirring, adjust ph is 7, filter, add concentration be 10% salt acid for adjusting pH value be 3, static 3 hours, filter, filtrate reduced in volume obtains and gets dry extract, be ground into 100 object powder, by Spica Prunellae 22g, Herba Menthae 22g, Semen Euryales 27g, Fructus Corni 32g, Herba Stellariae Alsines 17g, Folium Symplocoris Caudatae 22g, Folium et Caulis Indigoferae Tinctoriae 22g mixes, obtain mixture, add the water of 450g, decoct 4 hours, decoction liquor is filtered, filtering residue adds the water of 450g again, decoct 3 hours, decoction liquor is filtered, twice decoction liquor is merged, add concentration be 10% salt acid for adjusting pH value be 3, static 3 hours, filter, filtrate alcohol extraction, extract concentrating under reduced pressure becomes dry mastic, dry subsequently, be ground into 100 object powder, this powder is mixed with the powder obtained above, obtain fine powder, by the sodium chloride of 2g, 1g mannitol, 0.06g hydroxy benzenes ethyl ester, add in appropriate sterilized water for injection, stir, add the fine powder obtained subsequently above, stirring and dissolving is complete, stir evenly, continue to add sterilized water for injection, to the crude drug concentration of eye drop is 1g/ml, after stirring, with filtering with microporous membrane, flowing steam sterilization 30min is used at 100 DEG C, subpackage under aseptic condition, obtain eye drop 3.

Embodiment 7 toxicology test

Its mouse oral is tested

Select Kunming kind body weight 18 ~ 22g regular grade mice 60, random point 3 groups, often organize 20, male and female half and half.Matched group adopts routine feeding, tests 1 group of decoction liquor 1 that on the basis of routine feeding prepared by gavage embodiment 1, tests 2 groups of eye drops 1 that on the basis of routine feeding prepared by gavage embodiment 4.Fasting 12h, presses 0.4ml/10g (body weight) disposable gastric infusion to each group of mice.Viewing test animal poisoning manifestations and death condition after administration, Continuous Observation 14d.Calculate LD value.Result of the test shows, chmice acute Oral toxicity is tested, and presses once method to greatest extent, the equal > 10g/kg of LD, true border non-toxic type.

An intact skin irritation test

By healthy adult white, man exempts from 12, body weight 2.5 ~ 2.7kg, male and female half and half, is divided into test 1 group and test 2 groups.By its spinal column both sides skin unhairing 3cm × 3cm, getting 2 pieces of 2.5cm × 2.5cm, bis-layers of gauze is immersed in the decoction liquor 1 of embodiment 1 preparation and the eye drop 1 of embodiment 4 preparation, be applied on test 1 group and test 2 groups of plucked skins in family's rabbit back side respectively, cover with one deck cellophane, fixed with nonirritant medical proof fabric and binder, opposite side distilled water compares again.Every experimental animal is applied ointment or plaster after 4h, removes tested material, with the skin of warm water cleaning application site, respectively at 1,24,48h observes and records the dermoreaction of application site, the standards of grading specified according to " disinfection technology standard " and classification are marked.Irritation test shows, Chinese medicinal ointment provided by the invention 1 pair of rabbit skin wound repair integration is 0, and skin, without erythema, belongs to non-stimulated.

Embodiment 8 clinical data

Physical data

180 routine patients all carry out the ophthalmology outpatient service of self-institute, and totally 360 eyes, are divided into two groups at random by random digits table.Treatment group 90 example (180 eyes), wherein male 55 example, women 35 example; 8 ~ 62 years old age, the course of disease 2 ~ 7 days.Matched group 90 example (180 eyes), wherein male 53 example, women 37 example; 7 ~ 65 years old age, the course of disease 1 ~ 7 day.Two groups in sex, age, the course of disease etc. through statistical test no significant difference (P > 0.05), there is comparability.

Diagnostic criteria

Subjective symptoms: patient's eyes priority or the puckery pain that simultaneously goes out to lose face, photophobia is shed tears, foreign body sensation, has a large amount of watery secretion.Examination of eyes: blepharoedema, palpebral conjunctiva and bulbar conjunctiva height congestion and edema, palpebral conjunctiva is shown in that a large amount of follicle hypertrophy or pseudomembrane are formed, see under bulbar conjunctiva that point, lamellar are hemorrhage, person sees that the multiple point-like of corneal epithelium comes off to involve cornea, fluorescent staining, can with preauricular lymph nodes enlargement, tenderness in being dispersed in or the some slice colouring of dense arrangement.

Therapeutic Method

Treatment group: first washout suffers from eye 5 minutes before the treatment.

Nebulae inhalation: by decoction liquor 1 nebulizer container of falling people prepared by embodiment 1, plastic nozzle is connected by mist pipe, nozzle is just to trouble eye, patient opens eyelid and accepts treatment, make medicinal liquid form atomization molecule by supersonic vibration and arrive bulbar conjunctiva and cornea, distance is 20cm, and start timing 20 minutes, is atomized the clean napkin of complete use and dries.

Eye drops in treatment: eye drop 1 prepared by embodiment 4 instill suffer from eye place, every day 3 times, the morning, noon and afternoon respectively once, each 1 ~ 2.

Nebulae inhalation and eye drops in treatment carry out simultaneously, within 7 days, are a course for the treatment of.

Matched group: give normal saline washout and suffer from eye 5min, every day 1 time; 0.1% hydrobenzole eye liquid eye dripping, 1 time per hour, 0.3% norfloxacin eye liquid eye dripping, every 2 hours 1 time 7 days is 1 course for the treatment of.Tetracycline eye ointment is used evening to be coated with eye.

Criterion of therapeutical effect:

Cure: the puckery pain of eye, the symptoms such as photophobia is shed tears, foreign body sensation disappear completely, and without secretions, blepharoedema, palpebral conjunctiva and bulbar conjunctiva congestion and edema disappear, and corneal fluorescein is negative staining.Take a turn for the better: the puckery pain of eye, the symptoms such as photophobia is shed tears, foreign body sensation obviously alleviate, and without secretions, blepharoedema, palpebral conjunctiva and bulbar conjunctiva congestion and edema obviously alleviate, and corneal fluorescein dyeing reduces.Do not heal: medication is after 7 days, and symptom is without obviously alleviating.

Statistical method

Measurement data adopts represent, compare between group and adopt x ²inspection, all data all adopt the process of SPSS 12.0 statistical software.

Result

Two groups of comparitive study, the results are shown in Table 1.

Table 1 liang group comparitive study

Group	N (eye)	Cure	Take a turn for the better	Do not heal	Effective percentage/%
						Treatment group	180	151	27	2	98.9
Matched group	180	108	43	29	83.9

As can be seen from Table 1, adopt medicine provided by the invention respond well in treatment hemorrhagic conjunctivitis.

The course for the treatment of:

For the patient cured, the average curative day for the treatment of group is 2.63 days, and the average curative day of matched group is 6.19 days, will be significantly shorter than the course for the treatment of of matched group the course for the treatment of of treatment group.

All above-mentioned this intellectual properties of primary enforcement, not setting restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.

The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.

Claims (7)

1. be used for the treatment of a medicine for epidemic hemorrhagic conjunctivitis, it is characterized in that: crude drug comprises Concha Haliotidis, Cortex Phellodendri, Flos Lonicerae, the Radix Pulsatillae, Spica Prunellae, Herba Menthae, Semen Euryales, Fructus Corni, Herba Stellariae Alsines, Folium Symplocoris Caudatae, Folium et Caulis Indigoferae Tinctoriae, Radix Rubiae, Herba Mimosae Pudicae, Flos Trollii, Elephantopus scaber L., Rhizoma Polygonati, the Radix Paeoniae Alba and Radix Glycyrrhizae.

2. medicine as claimed in claim 1, is characterized in that: the weight of each crude drug is respectively Concha Haliotidis 25g ~ 35g, Cortex Phellodendri 45g ~ 55g, Flos Lonicerae 35g ~ 45g, Radix Pulsatillae 35g ~ 45g, Spica Prunellae 15g ~ 25g, Herba Menthae 15g ~ 25g, Semen Euryales 20g ~ 30g, Fructus Corni 25g ~ 35g, Herba Stellariae Alsines 10g ~ 20g, Folium Symplocoris Caudatae 15g ~ 25g, Folium et Caulis Indigoferae Tinctoriae 15g ~ 25g, Radix Rubiae 25g ~ 35g, Herba Mimosae Pudicae 35g ~ 45g, Flos Trollii 30g ~ 40g, Elephantopus scaber L. 25g ~ 35g, Rhizoma Polygonati 30g ~ 40g, Radix Paeoniae Alba 15g ~ 25g and Radix Glycyrrhizae 20g ~ 30g.

3. medicine as claimed in claim 1 or 2, is characterized in that: the weight of each crude drug is respectively Concha Haliotidis 28 ~ 32g, Cortex Phellodendri 48g ~ 52g, Flos Lonicerae 38g ~ 42g, Radix Pulsatillae 38g ~ 42g, Spica Prunellae 18g ~ 22g, Herba Menthae 18g ~ 22g, Semen Euryales 23g ~ 27g, Fructus Corni 28g ~ 32g, Herba Stellariae Alsines 13g ~ 17g, Folium Symplocoris Caudatae 18g ~ 22g, Folium et Caulis Indigoferae Tinctoriae 18g ~ 22g, Radix Rubiae 28g ~ 32g, Herba Mimosae Pudicae 38g ~ 42g, Flos Trollii 33g ~ 37g, Elephantopus scaber L. 28g ~ 32g, Rhizoma Polygonati 33g ~ 37g, Radix Paeoniae Alba 18g ~ 22g and Radix Glycyrrhizae 23g ~ 27g.

4. the medicine as described in claims 1 to 3, is characterized in that: the weight of each crude drug is respectively Concha Haliotidis 30g, Cortex Phellodendri 50g, Flos Lonicerae 40g, Radix Pulsatillae 40g, Spica Prunellae 20g, Herba Menthae 20g, Semen Euryales 25g, Fructus Corni 30g, Herba Stellariae Alsines 15g, Folium Symplocoris Caudatae 20g, Folium et Caulis Indigoferae Tinctoriae 20g, Radix Rubiae 30g, Herba Mimosae Pudicae 40g, Flos Trollii 35g, Elephantopus scaber L. 30g, Rhizoma Polygonati 35g, Radix Paeoniae Alba 20g and Radix Glycyrrhizae 25g.

5. the medicine as described in Claims 1-4, is characterized in that: described medicine can be prepared into decoction liquor and eye drop.

6. the preparation method of medicine described in claim 1 to 5, is characterized in that: when prepared medicine is decoction liquor, is specially and each crude drug component is pulverized respectively, put into blender to be uniformly mixed, obtain mixture, add the water relative to mixture quality 2 ~ 4 times, decoct 3 ~ 5 hours, decoction liquor is filtered, filtering residue adds the water relative to the mixture quality prepared 2 ~ 4 times again above, decocts 2 ~ 4 hours, and decoction liquor is filtered, twice decoction liquor is merged, obtains decoction liquor.

7. the preparation method of medicine described in claim 1 to 5, is characterized in that: when prepared medicine is eye drop, be specially:

The first step, by Concha Haliotidis, Cortex Phellodendri, Flos Lonicerae, the Radix Pulsatillae, Radix Rubiae, Herba Mimosae Pudicae, Flos Trollii, Elephantopus scaber L., Rhizoma Polygonati, the Radix Paeoniae Alba and Radix Glycyrrhizae are by above-mentioned weight mixing, with the alcohol reflux 2 times ~ 4 times that determining alcohol is 80% ~ 90%, the amount of the ethanol added is the mixture quality 4 ~ 6 times that this step obtains, filter, merging filtrate, be evaporated to, when 60 DEG C, relative density is the mastic of 1.15 ~ 1.20, add the water dissolution relative to mastic quality 3 ~ 5 times amount subsequently, add relative to mass percentage concentration 5% ~ 10% sodium hydrate aqueous solution hydrotropy under stirring, adjust ph is 7 ~ 8, filter, add concentration be 10% salt acid for adjusting pH value be 3 ~ 4, static 2 ~ 4 hours, filter, filtrate reduced in volume obtains and gets dry extract, be ground into 50 order ~ 100 object powder,

Second step, by remaining components by above-mentioned weight mixing, obtain mixture, add the water relative to mixture quality 2 ~ 4 times, decoct 3 ~ 5 hours, decoction liquor is filtered, filtering residue adds the water relative to the mixture quality prepared 2 ~ 4 times again above, decoct 2 ~ 4 hours, decoction liquor is filtered, twice decoction liquor is merged, add concentration be 10% salt acid for adjusting pH value be 3 ~ 4, static 2 ~ 4 hours, filter, filtrate alcohol extraction, extract concentrating under reduced pressure becomes dry mastic, dry subsequently, be ground into 50 order ~ 100 object powder, the powder that this powder and the first step obtain is mixed, obtain fine powder,

3rd step, by the sodium chloride of 1g ~ 2g, 0.5g ~ 1g mannitol, 0.03g ~ 0.06g hydroxy benzenes ethyl ester, add in appropriate sterilized water for injection, stir, add the fine powder that second step obtains subsequently, stirring and dissolving is complete, stir evenly, continue to add sterilized water for injection, to the crude drug concentration of eye drop is 1g/ml, after stirring, with filtering with microporous membrane, at 100 DEG C, use flowing steam sterilization 30min, subpackage under aseptic condition.