CN104262112A - 从花生中提取白藜芦醇的方法 - Google Patents

从花生中提取白藜芦醇的方法 Download PDF

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CN104262112A
CN104262112A CN201410423469.6A CN201410423469A CN104262112A CN 104262112 A CN104262112 A CN 104262112A CN 201410423469 A CN201410423469 A CN 201410423469A CN 104262112 A CN104262112 A CN 104262112A
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resveratrol
trans
peanuts
peanut
extraction
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王耀斌
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Shaanxi Shengmai Petroleum Co Ltd
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Shaanxi Shengmai Petroleum Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
    • C07C37/68—Purification; separation; Use of additives, e.g. for stabilisation
    • C07C37/685—Processes comprising at least two steps in series
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
    • C07C37/68—Purification; separation; Use of additives, e.g. for stabilisation
    • C07C37/70—Purification; separation; Use of additives, e.g. for stabilisation by physical treatment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
    • C07C37/68—Purification; separation; Use of additives, e.g. for stabilisation
    • C07C37/70—Purification; separation; Use of additives, e.g. for stabilisation by physical treatment
    • C07C37/82—Purification; separation; Use of additives, e.g. for stabilisation by physical treatment by solid-liquid treatment; by chemisorption
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/203—Monocyclic carbocyclic rings other than cyclohexane rings; Bicyclic carbocyclic ring systems

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  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Seeds, Soups, And Other Foods (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

本发明涉及一种白藜芦醇的制备方法，属于食品添加剂及其制备方法领域。所述的从花生中提取白藜芦醇的方法，包括以下步骤：(1)将带皮花生烘烤，用粉碎机将其粉碎，按一定的料水比将粉碎花生悬浮于乙醇中，高速均质后离心除去悬浮液中悬浮物颗粒，将滤液减压浓缩；(2)将浓缩液通过含Al2O3的60R18硅胶柱，并用无水乙醇洗脱；(3)将洗脱液用氮气蒸干，得粉末即白藜芦醇。本发明所述的从花生中提取白藜芦醇的方法，具有工艺简单、可操作性强，适用于工业化生产以及回收率、成本低等特点。

Description

从花生中提取白藜芦醇的方法
技术领域
本发明涉及一种白藜芦醇的制备方法，属于食品添加剂及其制备方法领域。
背景技术
白藜芦醇是多酚类化合物，主要来源于葡萄（红葡萄酒）、虎杖、花生、桑椹等植物。白藜芦醇是一种生物性很强的天然多酚类物质，又称为芪三酚，是肿瘤的化学预防剂，也是对降低血小板聚集，预防和治疗动脉粥样硬化、心脑血管疾病的化学预防剂。美国农业部的研究结果表明，花生红衣与仁中也含有相当多的白藜芦醇。白藜芦醇的实验研究已经证实具有对心血管疾病和癌症的有益作用。白藜芦醇对激素依赖性肿瘤有明显的预防作用。还可对骨质疏松、痤疮及老年痴呆症有预防作用，具有抗病毒及免疫调节作用。对人体内部一种单体抗衰老酶起作用，进而发挥预防各种年龄相关疾病、延长预期寿命的潜在作用。
因此，研究一种从花生中提取白藜芦醇具有明显的经济和社会效益。
发明内容
本发明旨在提供一种工艺简单、可操作性强，适用于工业化生产以及回收率、成本低的从花生中提取白藜芦醇的方法。
本发明所述的从花生中提取白藜芦醇的方法，包括以下步骤：
(1)将带皮花生烘烤，用粉碎机将其粉碎，按一定的料水比将粉碎花生悬浮于乙醇中，高速均质后离心除去悬浮液中悬浮物颗粒，将滤液减压浓缩；
(2)将浓缩液通过含Al2O3的60R18硅胶柱，并用无水乙醇洗脱；
(3)将洗脱液用氮气蒸干，得粉末即白藜芦醇。
优选的，本发明所述的步骤（1）中的烘烤温度为160-170℃，烘烤时间为2-3h，乙醇浓度为80％(v/v),料水比为3:1(w/v)；
更优选的，本发明所述的步骤（2）中的硅胶柱与Al2O3的重量比为1:1；洗脱温度为70-85℃；
进一步优选的，本发明所述的步骤（3）中的氮气蒸干温度为55-65℃。
本发明所述的提取方法制得的白藜芦醇，经测得不同品种花生中白藜芦醇含量在0.03-0.147μg/g之间，而尤以带皮花生中较高，皮中白藜芦醇含量在0.5—0.78μg/g。经TLC及HPLC分析，确定其成分为：反式白藜芦醇、顺式白藜芦醇及反式白藜芦醇苷与顺式白藜芦醇苷。
本发明所述的从花生中提取白藜芦醇的方法，具有工艺简单、可操作性强，适用于工业化生产以及回收率、成本低等特点。
具体实施方式
实施例一：
将10kg带皮花生于176℃下烘烤，用粉碎机将其粉碎后，按3:1(w/v)比例将粉碎花生悬浮于80％v/v乙醇中(即花生含量为3g/m1)，高速均质后离心除去提取物中悬浮物颗粒，减压浓缩；将浓缩液过含有重量比1:1（w/w）Al2O3的60R18硅胶柱，在80℃下无水乙醇洗脱，将洗脱液用氮气于60℃下蒸干，得粉末即白藜芦醇1.23g。经TLC及HPLC分析，确定其成分为：反式白藜芦醇、顺式白藜芦醇及反式白藜芦醇苷与顺式白藜芦醇苷。 

Claims (4)

1.从花生中提取白藜芦醇的方法，包括以下步骤：
(1)将带皮花生烘烤，用粉碎机将其粉碎，按一定的料水比将粉碎花生悬浮于乙醇中，高速均质后离心除去悬浮液中悬浮物颗粒，将滤液减压浓缩；
(2)将浓缩液通过含Al2O3的60R18硅胶柱，并用无水乙醇洗脱；
(3)将洗脱液用氮气蒸干，得粉末即白藜芦醇。
2. 如权利要求1所述的从花生中提取白藜芦醇的方法，其特征在于所述的步骤(1) 中的烘烤温度为160-170℃，烘烤时间为2-3h，乙醇浓度为80％(v/v),料水比为3:1(w/v)。
3. 如权利要求1所述的从花生中提取白藜芦醇的方法，其特征在于所述的步骤（2）中的硅胶柱与Al2O3的重量比为1:1；洗脱温度为70-85℃。
4. 如权利要求1所述的从花生中提取白藜芦醇的方法，其特征在于所述的步骤（3）中的氮气蒸干温度为55-65℃。
CN201410423469.6A 2014-08-26 2014-08-26 从花生中提取白藜芦醇的方法 Pending CN104262112A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3243828A4 (en) * 2015-01-08 2018-09-05 KPC Pharmaceuticals, Inc. Method for extracting stilbene compounds

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3243828A4 (en) * 2015-01-08 2018-09-05 KPC Pharmaceuticals, Inc. Method for extracting stilbene compounds
US10307453B2 (en) 2015-01-08 2019-06-04 Kpc Pharmaceuticals, Inc Method for extracting stilbene compounds

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