CN104206241A - Method for cutting cypripedium franchetii - Google Patents
Method for cutting cypripedium franchetii Download PDFInfo
- Publication number
- CN104206241A CN104206241A CN201410463180.7A CN201410463180A CN104206241A CN 104206241 A CN104206241 A CN 104206241A CN 201410463180 A CN201410463180 A CN 201410463180A CN 104206241 A CN104206241 A CN 104206241A
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- cuttage
- lady
- slipper
- cutting
- hair
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- Y02P60/216—
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- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for cutting cypripedium franchetii. The cutting method comprises the following steps: collecting and treating cutting braches, preparing a seedling bed and base materials, carrying out cutting mutation, carrying out post-management after cutting and the like. By adopting the method disclosed by the invention, the survival rate of the cypripedium franchetii is high, the mutation rate is high, the cost is low and the content of secondary metabolites is high.
Description
Technical field
The cuttage that the present invention relates to mao lady's slipper is cultivated, and belongs to field of plant growing technology.
Background technology
Hair lady's slipper,
cypripedium franchetiithe orchid family, produce SOUTH OF GANSU, Southern Shanxi Province, Southern Shaanxi, West Henan, West of Hubei Province and Sichuan region to the northwestward, under being born in the sparse woods of height above sea level 1500-3700 rice or moistening in spinney, humus is abundant and well-drained place, also seeing on moistening careless slope, is the plant that a kind of medical value is higher.Mainly field acquisition at present, availability is low, and cottage method is simple to operate, and genetic stability is good, and the exploitation for hair lady's slipper provide good foundation.
Summary of the invention
Technical problem to be solved by this invention is to provide the cottage method of a kind of mao of lady's slipper, and adopt the hair lady's slipper survival rate of the inventive method high, induced mutation rate is high, and cost is low, and Secondary Metabolite Contents is high.
Technical problem to be solved by this invention is realized by following scheme:
Select the cutting of hair lady's slipper semi-lignified robust growth, running water 3 minutes, 35% alcohol+3% disappears clean clever mixed liquid dipping 7min, clear water rinses 4-5 time, and in 50 ± 1 DEG C of hot-water soak 2h, branch morphology lower end 2-3cm is soaked in water culture sprout in 1/2MS solution, the morphology lower end oblique cutting of hair lady's slipper is entered rural area soil: vermiculite: in sawdust=3:1:1 matrix, oblique cutting angle 45, cuttage seeding row spacing 15cm × 16cm, regularly uses
60co-gamma-radiation, use automatic sprinkler after cuttage, within every three days, spray a 90ppmNAA solution, spray 6 times altogether, every carbendazim solution spraying 600 times for two weeks, spray three times altogether, temperature 28 DEG C, intensity of illumination 5000lx, every day light application time 8h, PH5.8-6.0, humidity 85%, added up survival rate after three months.
The hair lady's slipper survival rate adopting the present invention to prepare is 86%, and the cycle is short, and cost is low, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
Select the cutting of hair lady's slipper semi-lignified robust growth, running water 3 minutes, 35% alcohol+3% disappears clean clever mixed liquid dipping 7min, clear water rinses 4-5 time, and in 50 ± 1 DEG C of hot-water soak 2h, branch morphology lower end 2-3cm is soaked in water culture sprout in 1/2MS solution, the morphology lower end oblique cutting of hair lady's slipper is entered rural area soil: vermiculite: in sawdust=3:1:1 matrix, oblique cutting angle 45, cuttage seeding row spacing 15cm × 16cm, regularly uses
60co-gamma-radiation, use automatic sprinkler after cuttage, within every three days, spray a 60ppmNAA solution, spray 6 times altogether, every carbendazim solution spraying 600 times for two weeks, spray three times altogether, temperature 28 DEG C, intensity of illumination 5000lx, every day light application time 8h, PH5.8-6.0, humidity 85%, adds up survival rate 82% after three months.
Embodiment 2
Select the cutting of hair lady's slipper semi-lignified robust growth, running water 3 minutes, 35% alcohol+3% disappears clean clever mixed liquid dipping 7min, clear water rinses 4-5 time, and in 50 ± 1 DEG C of hot-water soak 2h, branch morphology lower end 2-3cm is soaked in water culture sprout in 1/2MS solution, the morphology lower end oblique cutting of hair lady's slipper is entered rural area soil: vermiculite: in sawdust=3:1:1 matrix, oblique cutting angle 45, cuttage seeding row spacing 15cm × 16cm, regularly uses
60co-gamma-radiation, use automatic sprinkler after cuttage, within every three days, spray a 100ppmNAA solution, spray 6 times altogether, every carbendazim solution spraying 600 times for two weeks, spray three times altogether, temperature 28 DEG C, intensity of illumination 5000lx, every day light application time 8h, PH5.8-6.0, humidity 85%, adds up survival rate 83% after three months.
Embodiment 3
Select the cutting of hair lady's slipper semi-lignified robust growth, running water 3 minutes, 35% alcohol+3% disappears clean clever mixed liquid dipping 7min, clear water rinses 4-5 time, and in 50 ± 1 DEG C of hot-water soak 2h, branch morphology lower end 2-3cm is soaked in water culture sprout in 1/2MS solution, the morphology lower end oblique cutting of hair lady's slipper is entered rural area soil: vermiculite: in sawdust=3:1:1 matrix, oblique cutting angle 45, cuttage seeding row spacing 15cm × 16cm, regularly uses
60co-gamma-radiation, use automatic sprinkler after cuttage, within every three days, spray a 80ppmNAA solution, spray 6 times altogether, every carbendazim solution spraying 600 times for two weeks, spray three times altogether, temperature 28 DEG C, intensity of illumination 5000lx, every day light application time 8h, PH5.8-6.0, humidity 85%, adds up survival rate 84% after three months.
Claims (3)
1. a method for hair lady's slipper cuttage, is characterized in that:
(1) Acquire and process of cuttage branch: the cutting selecting hair lady's slipper semi-lignified robust growth, after 50 ± 1 DEG C of hot-water soak 2h, branch morphology lower end 2-3cm is soaked in water culture sprout in 1/2MS solution;
(2) preparation of seedbed and matrix: the cutting medium of hair lady's slipper selects rural area soil: vermiculite: sawdust=3:1:1;
(3) cuttage mutagenesis: enter in matrix by the morphology lower end oblique cutting of hair lady's slipper, oblique cutting angle 45, cuttage seeding row spacing 15cm × 16cm, regularly uses
60co-gamma-radiation;
(4) manage after cuttage: after cuttage, use automatic sprinkler, within every three days, spray a 70-100ppmNAA solution, spray 6 times altogether, every carbendazim solution spraying 600 times for two weeks, spray three times altogether, after three months, add up survival rate.
2. the method for a kind of mao of lady's slipper cuttage according to claim 1, to is characterized in that in step (1) before cuttage branch cutting running water 3 minutes, and 35% alcohol+3% disappears clean clever mixed liquid dipping 7min, and clear water rinses 4-5 time.
3. the method for a kind of mao of lady's slipper cuttage according to claim 1, is characterized in that cuttage management condition is temperature 28 DEG C in step (4), intensity of illumination 5000lx, every day light application time 8h, PH5.8-6.0, humidity 85%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410463180.7A CN104206241A (en) | 2014-09-12 | 2014-09-12 | Method for cutting cypripedium franchetii |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410463180.7A CN104206241A (en) | 2014-09-12 | 2014-09-12 | Method for cutting cypripedium franchetii |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104206241A true CN104206241A (en) | 2014-12-17 |
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ID=52088463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410463180.7A Pending CN104206241A (en) | 2014-09-12 | 2014-09-12 | Method for cutting cypripedium franchetii |
Country Status (1)
| Country | Link |
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| CN (1) | CN104206241A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105815121A (en) * | 2016-03-25 | 2016-08-03 | 江苏省林业科学研究院 | Novel method for grafting oil paeonia rockii |
| CN107211855A (en) * | 2017-06-12 | 2017-09-29 | 胡柳新 | The implantation methods of kaffir lily |
-
2014
- 2014-09-12 CN CN201410463180.7A patent/CN104206241A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105815121A (en) * | 2016-03-25 | 2016-08-03 | 江苏省林业科学研究院 | Novel method for grafting oil paeonia rockii |
| CN107211855A (en) * | 2017-06-12 | 2017-09-29 | 胡柳新 | The implantation methods of kaffir lily |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141217 |