CN103444501A

CN103444501A - Cuttage propagation method for plumbago auriculata

Info

Publication number: CN103444501A
Application number: CN2013103824210A
Authority: CN
Inventors: 高素萍; 张硕; 雷霆; 周玲云; 吴佩纹; 张开会; 林萧
Original assignee: Sichuan Agricultural University
Current assignee: Sichuan Agricultural University
Priority date: 2013-08-29
Filing date: 2013-08-29
Publication date: 2013-12-18

Abstract

The invention relates to the technical field of plant cultivation, in particular to the technical field of plumbago auriculata cuttage. The technical scheme aims at providing a cuttage propagation method for a plumbago auriculata. The cuttage method of the plumbago auriculata comprises the steps of shearing middle-upper hard stems of the plumbago auriculata as cutting slips with the length of 10-12cm; preparing a transplanting bed matrix by roseite and river sand with the preparation proportion of 1:1; after soaking the hard stems of the plumbago auriculata into a solution containing 500mg/L of IAA (Indoleacetic Acid), 500mg/L of IBA (Indole Butyric Acid), and 1000mg/L of NAA (Naphthalene Acetic Acid) for 30 seconds, planting the hard stems of the plumbago auriculata into the transplanting bed, and pouring water over the hard stems. The cost of the plumbago auriculata cut by the method can be greatly reduced, the rate of survival is high and can reach up to 92.22%, the rooting time is earlier, the growth and development is fast, and the reproductive cycle of the plumbago auriculata can be reduced, so that a new high-efficient propagation method can be provided for the production of the plumbago auriculata; the cuttage propagation method is simple and feasible, and is easy to popularize.

Description

Indigo plant is spent red cuttage breeding method

Technical field

The present invention relates to field of plant growing technology, in particular indigo plant is spent red cuttage breeding method.

Background technology

Lan Huadan (Plumbago auriculata) originates in South Africa, the perennial shrub of Plumbaginaceae leadwort.Indigo plant is spent red pattern pale blue, is rare cool tone flowers, and the florescence is long, so Lan Huadan has very high ornamental plantation, is worth.Lan Huadan or Africa and European countries obtain the important sources of medicinal ingredient plumbagin.Indigo plant spends red root to be rich in plumbagin.Plumbagin is that the Plumbaginaceae plant has one of important activity composition of antitumor action, has antibiotic, anti-inflammatory, the effect such as anticancer.

At present, Europe and Africa, because large tracts of land collection indigo plant is spent the red plumbagin that extracts, make this species natural resources exhausted rapidly.Lan Huadan is the heterostyly plant, has not affinity phenomenon of selfing, and under natural conditions, ripening rate is low, and in the face of breach huge between the market demand and plant resources, breeding problem becomes the matter of utmost importance of Lan Huadan research.

The propagation method of Lan Huadan is divided into sexual propagation and vegetative propagation.Sexual propagation is mainly seed propagation, and domestic indigo plant is spent red as alien species, the main import provenance that relies on of breeding, and cost is high.

Vegetative propagation is divided into again cuttage, grafting, press strip, plant division.Wherein because of cottage propagation, having the characteristics such as fast, the small investment of emerging is commonly used.So the present invention adopts the method for cuttage to shorten indigo plant and spends red growth cycle, reduces costs, and spends red cottage propagation for indigo plant the theory and practice foundation is provided.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of indigo plant to spend red cuttage breeding method, thereby reduce indigo plant, spends red reproductive-cost, shortens its growth cycle, meets the domestic market application demand.

The invention provides a kind of indigo plant and spend red cuttage breeding method, comprise the following steps:

The processing of A, slotting machine: after improvement, osculum is made in the every 1.5～2.0m box out of base portion of slotting machine, the thick cobblestone of bed end paving one deck 15～20cm, to facilitate plant draining, ventilative, above cover the thick thin river sand of one deck 10cm, upper strata repaves the river sand of 15cm and vermiculite (1:1) as cutting medium.Matrix liquor potassic permanganate spray with 0.3% before cuttage is sterilized, and after sealing sterilization 2h, with running water, drenches clean, then shakeouts standby;

B, cuttage branch are processed: spend red hard cuttings to be placed in IAA500mg/L+IBA500mg/L+NAA1000mg/L solution indigo plant and soak 30s;

C, cuttage: the branch of handling well is inserted in the cuttage cave, and reclaimed soil, water permeable;

After D, cuttage, manage: temperature remains on 18～28 ℃, and the water content of slotting machine remains on 60～70%, and relative air humidity is 65～75%, allows cuttage seeding accept gradually illumination.

Wherein the length of the described cuttage branch of step B is 10～12cm, and while gathering in field, every 20 are bundled into a bundle, in the middle of packing into, is placed with in the ice cube bubble chamber.After taking back greenhouse, the base portion of transplanting a cutting is put into the plastic big basin that is filled with water, and at the blade face water spray, the dehydration that prevents from transplanting a cutting is wilted in good time.

Wherein to spend the red hard the most suitable cutting time of stem cutting fringe be the first ten-day period of the March to the described indigo plant of step C.

The advantage that the present invention has with good effect is: with traditional seed propagation, compare, adopt the indigo plant of this method cuttage to spend red cost greatly to reduce, after the little transplantation of seedlings of taking root, root system is abundant, and survival rate is high, has shortened the breeding cycle of Lan Huadan, for the production of Lan Huadan provides new efficient propagation method, and operation is simple, be easy to promote, can large scale application.

The accompanying drawing explanation

Fig. 1: slotting machine elevation after improvement

Illustrate: osculum is made in the every 1.5～2.0m box out of the base portion of slotting machine, the thick cobblestone of bed end paving one deck 15～20cm, to facilitate plant draining, ventilative, above cover the thick thin river sand of one deck 10cm, upper strata repaves the river sand of 15cm and vermiculite (1:1) as cutting medium.

Embodiment

Below in conjunction with specific embodiment, the present invention is described in detail.

Embodiment 1

The present embodiment provides a kind of indigo plant to spend red cuttage breeding method, mainly using cottage propagation as technical support, select the hard stem cutting fringe of Lan Huadan, on the impact of cottage propagation test (combination of regulators formula in Table 1, table 2), finally selected the combination of regulators formula of one group of optimum with different growth regulators through optimum organization.

At first prepare slotting machine: the high 1.2m of slotting machine, long 5m, wide 1.0m, dark 0.6m.Osculum is made in the every 1.5～2.0m box out of slotting machine base portion, and the thick cobblestone of bed end paving one deck 15～20cm, above cover the thick thin river sand of one deck 10cm, and upper strata repaves the river sand of 15cm and vermiculite (1:1) as cutting medium.Matrix liquor potassic permanganate spray with 0.3% before cuttage is sterilized, and after sealing sterilization 2h, with running water, drenches clean, then shakeouts standby.

Branch collection and processing: after field gathers, every 20 are bundled into a bundle, in the middle of packing into, are placed with in the ice cube bubble chamber, prevent the passivation of cutting heating enzymic activity, affect the cuttage root-taking rate.After taking back the experiment greenhouse, the base portion of transplanting a cutting is put into the plastic big basin that is filled with water, and at the blade face water spray, the dehydration that prevents from transplanting a cutting is wilted in good time.

Early March carries out cuttage, 3 repetitions of every processing, and each repeats 30 cuttings.Indigo plant is spent in NAA, IAA that red hard cuttings is 500mg/L, 1000mg/L in concentration respectively, IBA solution and soaked 30s, with clear water (ck) in contrast.This experiment adopts Three factors-levels orthogonal L9 (34), and processing method is in Table 1,2.

Table 1. indigo plant is spent red cuttage to take root and is tested processing factor and horizontal list

Table 2 indigo plant is spent red cuttage root-taking experiment orthogonal experiment scheme L9 (3 ⁴)

Experimental result: as can be seen from Table 3, except processing 1,6,7,8, process 5 and other each process between difference extremely remarkable, therefore optimization process is combined as: IAA500mg/L+IBA500mg/L+NAA1000mg/L.

The different growth regulators of table 3. are processed the impact of on indigo plant, spending red cuttage to take root

Annotate: in table, the rear capitalization of numeral means 0.01 level difference significance.

Choosing best of breed (IAA500mg/L+IBA500mg/L+NAA1000mg/L) spends red hard branch to carry out the cuttage processing to indigo plant, 3 repetitions of every processing, each repeats 30 cuttings, after processing, implants slotting machine, it is 25 ℃/18 ℃ (day/night) that temperature remains on, illumination 12h.The water content of slotting machine remains on 60～70%, and relative air humidity is 65～75%.When root reaches 1.5cm, transplanted, after transplanting 15d, add up its survival rate.

Experimental result: as can be seen from Table 4, the survival rate of the Lan Huadan obtained according to the present invention is 92.22%.

Table 4. indigo plant is spent red cottage propagation survival rate

Embodiment 2

The present embodiment adopts conventional method breeding Lan Huadan, adopts seed propagation.

Choose the full seed without insect pest, the disinfecting solution of potassium permanganate 30min with 3%, after distilled water washes down, carry out the imbibition processing by seed.Each is processed 3 times and repeats, and each repeats 30 seeds, seed is laid in respectively to bottom and is covered with in the culture dish of moistening filter paper, puts into illumination box and cultivates, and cultivation temperature is 25 ℃/18 ℃ (day/night), illumination 12h.

Between culture period, with ionized water, keep the surface of the seed moistening, mouldy seed takes out to clean and puts back to, and removes the seed that rots.Observe seed sprouting and growing state every day, germinate grain number to measure root long of record, observe the seed sprouting number for 2 times until continuous and be 0, and the radicle of usining is prominent breaks in the seed coat 2mm as the sprouting standard, when root reaches 1.5cm, transplants, and after transplanting 15d, adds up its survival rate.

Experimental result: as seen from Table 5, the survival rate of the Lan Huadan that adopts said method to obtain is 83.33%.

Table 5. indigo plant is spent red seed propagation survival rate

As can be seen from Table 6: adopt the Lan Huadan of this method cuttage to compare with traditional seed propagation method, cost used reduces greatly, and survival rate is high.And the indigo plant that adopts this method cuttage spends red rootage duration early, grow fast, shortened the breeding cycle of Lan Huadan, simplified the cultivation operation, for the production of Lan Huadan provides new efficient propagation method, greatly improved production efficiency, operation is simple for this method, be easy to promote, meet large scale application.

Table 6. indigo plant is spent red seed propagation and cottage propagation relatively

Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.

Claims

1. indigo plant is spent red cuttage breeding method, it is characterized in that:

The processing of A, slotting machine: after improvement, osculum is made in the every 1.5～2.0m box out of base portion of slotting machine, and the thick cobblestone of bed end paving one deck 15～20cm, above cover the thick thin river sand of one deck 10cm, and upper strata repaves the river sand of 15cm and vermiculite (1:1) as cutting medium.Matrix liquor potassic permanganate spray with 0.3% before cuttage is sterilized, and after sealing sterilization 2h, with running water, drenches clean, then shakeouts standby;

2. indigo plant according to claim 1 is spent red cuttage breeding method, it is characterized in that: the length of the described cuttage branch of step B is 10～12cm, and while gathering in field, every 20 are bundled into a bundle, in the middle of packing into, is placed with in the ice cube bubble chamber.After taking back greenhouse, the base portion of transplanting a cutting is put into the plastic big basin that is filled with water, and at the blade face water spray, the dehydration that prevents from transplanting a cutting is wilted in good time.

3. indigo plant according to claim 1 is spent red cuttage breeding method, it is characterized in that: it is the first ten-day period of the March that the described indigo plant of step C is spent the red hard the most suitable cutting time of stem cutting fringe.