CN104189883A

CN104189883A - Manufacturing method for inclusion body hepatitis resistant traditional Chinese medicine composition

Info

Publication number: CN104189883A
Application number: CN201410512863.7A
Authority: CN
Inventors: 曲伟青; 申玉军; 于瀚学; 孙振洲; 孙强
Original assignee: Qingdao Tianrui Ecological Technology Co Ltd
Current assignee: Qingdao Tianrui Ecological Technology Co Ltd
Priority date: 2014-09-29
Filing date: 2014-09-29
Publication date: 2014-12-10

Abstract

The invention provides a manufacturing method for an inclusion body hepatitis resistant traditional Chinese medicine composition. The inclusion body hepatitis resistant traditional Chinese medicine composition is characterized by adopting the following components: rhizoma cimicifugae, artemisia apiacea, sedum sarmentosum, radix scutellariae, cape jasmine fruit, white peony root, pericarpium citri reticulatae viride, radix codonopsis, fructus lycii, folium artemisiae argyi, gallnut, panax notoginseng, red beans, houttuynia cordata, coptis, eclipta alba, zingiber, loranthus parasiticus and radix glycyrrhizae; the manufacturing method comprises the following steps: performing extraction through a water extraction method for three times after mixing the components; cooking to obtain a ropy mixture after merging three time extraction liquids, and adding modified starch, hydroxymethyl cellulose and a sweetener; drying for obtaining powder; after disinfecting the powder for 10 seconds, adding glucose powder and gentamicin sulphate powder, wherein the mass ratio of the glucose powder to the gentamicin sulphate powder is 3:1, and the mass sum of the glucose powder and the gentamicin sulphate powder is 1% of the quality of the powder. The manufacturing method for the inclusion body hepatitis resistant traditional Chinese medicine composition is simple, convenient to use, and good in the effect for preventing and curing inclusion body hepatitis, and can obviously reduce the loss brought for a farmer due to the inclusion body hepatitis.

Description

A kind of manufacture method of Chinese medicine composition of anti-inclusion body hepatitis

technical field:

The present invention relates to chicken fowl cultural technique field, relate in particular to a kind of manufacture method of Chinese medicine composition of anti-inclusion body hepatitis.

background technology:

In the breeding process of chicken fowl, often because suffering from various diseases, cause chicken fowl dead, this treatment is too late, to raiser, bring huge economic loss, take chicken inclusion body hepatitis as example, principal character is that young chicken group mortality rate increases suddenly, the serious anemia of sick chicken, hepatomegaly, hemorrhage and downright bad.In visible liver cell nuclear, there is inclusion body.This disease claims again anemia syndrome.It is caused by fowl adenovirus, hemorrhage with liver, serious anemia, and jaundice, hemorrhage of muscle and mortality rate increase suddenly, and in hepatocyte, form the infectious disease that intranuclear inclusion is feature.The cause of disease of pathogenic characteristic chicken inclusion body hepatitis is the I group I fowl adenovirus of Adenoviridae.Virion is without cyst membrane, and diameter is 70-90 nanometers, and nucleic acid is distrand DNA.Virus, in endoreduplication, produces basophil inclusion bodies.Ether, chloroform, trypsin, 5% ethanol are had to resistance, and susceptible animal only has chicken susceptible, and broiler is multiple.Age of onset mostly occurs at the broiler in 3～7 week age, and laying hen also occasionally has generation.The sick chicken of the source of infection, be with malicious chicken.Virus is discharged virus by feces, trachea and nose and is infected healthy chicken.Mainly infect through respiratory tract, digestive tract and eye conjunctiva route of transmission, also can be transmitted to the next generation by hatching egg.Two seasons of spring and summer to occur more.The chicken of recovering can be obtained lifetime immunity.

Sick chicken is cutd open inspection, and liver is pale, matter is crisp, swelling, liver, skeleton, skeletal muscle have point-like or ecchymosis.In hepatocyte, there is inclusion body, rounded.Kidney, splenomegaly, the swelling of kidney height, is canescence.Clinical pathology main manifestations is liver enlargement, is khaki, and matter is crisp, and there are hemorrhage speckle in various degree, the necrosis region sometimes differing in size as seen in surface.Some cases can be seen bone marrow lesion, and femur bone marrow is hemorrhage is pink, and meanwhile, chest muscle and lower limb flesh are pale and have hemorrhage speckle, and subcutaneous tissue, fatty tissue and intestinal serous coat, mucosa are obviously hemorrhage as seen.Through 1～2 day incubation period of chicken of natural infection, it is dead that initial stage loses any symptom, after 2～3 days, the sick chicken spirit of minority is depressed, drowsiness, wattle is faded, skin is yellow, subcutaneous have hemorrhage, have once in a while water sample loose stool, within 3～5 days, reach peak mortality, mortality rate reaches 15-30%, continue, after 3～5 days, to stop gradually.Primary disease incubation period, is generally no more than 4 days, and in growth chicken group, morbidity rapidly, often occurs suddenly dead chicken.Sick chicken heating, down in spirits, poor appetite, dysentery, drowsiness, feather is fluffy and disorderly, and bent lower limb is squatted vertical.The sick chicken of minority presents jaundice symptom.The young chicken in 4～10 week age, if in both feet paralysis, what before have on one's deathbed sends calling, and occurs the nervous symptoms such as a back to arch, is usually that anemia and spirit are depressed, the course of disease is 10～14 days.When the chicken in discontented 5 week age infects, during generally to 8 week age, can be completely recovered.Sickness rate can be up to 100%, and mortality rate from 2% to 10% is not etc., sometimes can reach 30%～40%.Infect 1 of primary disease, Japanese instar chickling presents serious Anemia.

At present chicken inclusion body hepatitis be there is no to very effective therapy, therefore, in breeding process, mainly with putting prevention first, the chicken house of primary disease occurs, in feedstuff, add compound vitamin and trace element.Conventional Therapeutic Method as, the clear spice of sense poison, 3% glucose+vitamin C drinking-water.Also available Ganshenkang (composition is: Radix Isatidis, the Radix Astragali, Herba Epimedii, Folium Isatidis, Radix Glycyrrhizae etc.) is endured water, the ratio that is watered in liquid medicine ratio 1 ︰ more than 8, and slow fire boils, boil latter 15 minutes, leach medicine juice, be blended into proper amount of clear water, be used in conjunction 4～5 days, use 3% glucose+vitamin C drinking-water simultaneously.Other disclosed methods, for example paper < < brief talks the diagnosis and treatment > > of chicken inclusion body hepatitis, it discloses several groups of therapeutic schemes, as astragalus polysaccharides+the liver benefiting protects kidney: be used in conjunction 3 days～5 days.Medication every day 6 hours, once-a-day, changes clear water after 6 hours.Special strong+3% glucose of PHA+ ribavirin+bodyguard, is used in conjunction 4 days.Chinese medicine formula: Folium Isatidis 60 g, Herba Artemisiae Scopariae 30 g, Fructus Gardeniae 45 g, Rhizoma Polygoni Cuspidati 30 g, Radix Et Rhizoma Rhei 20 g, Herba Plantaginis 35 g, every 100 kg feedstuffs mix 0.5～1.0 kg, and control secondary infection, to reduce death.Concrete Therapeutic Method can be used (2 milliliters every of vitamin C injections; containing 0.1 milligram); vitamin K3, K4 injection (1 milliliter every, containing 4 milligrams); (1 milliliter every of gentamycin; containing 20 milligrams) each 1, to 250 milliliters of cold water, allow disease chicken freely drink; 1 week course for the treatment of, drug withdrawal 3 days; Change 250 milliliters of cold water, allow disease chicken freely drink, 1 week course for the treatment of, drug withdrawal 3 days; Change 500 milliliters of cold water, drink drug withdrawal after 1 week.Herba Artemisiae Scopariae 600g, Radix Et Rhizoma Rhei 300g, Fructus Gardeniae 300g decoct after 2h, and filtering extracting liquid is blended in 200kg water, freely drinks.

By the retrieval of above-mentioned prior art, can find, the current treatment Chinese medicine for inclusion body hepatitis, Western medicine has certain effect, and still, Western medicine is most is conventional disease-resistant medicine, multiple chicken fowl diseases all can be general, cause chicken fowl Drug resistance to increase, and drug residue is higher, is unfavorable for people's edible safety.Therefore, some Chinese medicine succedaneum have been there are, as Chinese patent application 2009101665868 discloses a kind of for preventing and treat the Chinese medicine feed additive of chicken liver lesion, its component is 50 parts of Radix Scutellariaes, 25 parts of Rhizoma Coptidis, 60 parts of Cortex Phellodendris, 60 parts of Rhizoma Atractylodis Macrocephalae (parched)s, 70 parts of Radix Gentianaes, 80 parts of Semen Plantaginiss, 40 parts of Radix Bupleuri, 40 parts of Radix Angelicae Sinensis, 60 parts of Radix Rehmanniae, 60 parts, Poria, 80 parts of Herba Houttuyniae, 40 parts of Flos Chrysanthemis, 50 parts, Radix Glycyrrhizae.And for example Chinese patent application 2010102703573 discloses a kind of for preventing and treat the Chinese medicine feed additive of chicken liver lesion: the raw medicinal material of choosing and composition by weight thereof respectively: 15 parts of Radix Scutellariae, 15 parts of Rhizoma Coptidis, 10 parts of Radix Gentianaes, 10 parts of Herba Houttuyniae, 12 parts of Herba Plantaginiss, 15 parts of Radix Rehmanniae, 7 parts of Flos Chrysanthemis, 8 parts of Radix Isatidis.Its preparation method is to mix after pulverizing or pulverize respectively after above-mentioned raw materials medical material is mixed.And for example Chinese patent application 2013100743847 discloses a kind of Chinese medicine oral liquid for the treatment of chicken inclusion body hepatitis, and its constituent is: Folium Isatidis 13-17g, glue thigh blue 13-17g, Rhizoma Et Radix Notopterygii 8-12g, Herba Plantaginis 8-12g, Fructus Gardeniae 8-12g, Radix Peucedani 8-12g, Radix Astragali 3-7g, Rhizoma Polygoni Cuspidati 3-7g, Poria 3-7g, Flos Lonicerae 3-7g, Indigo Naturalis 3-7g, Radix Glycyrrhizae 3-7g.

Purport of the present invention on the basis of existing technology, provides a kind of method simple, easy to use, and prevention and cure chicken inclusion body hepatitis is effective, can obviously reduce the cultural method of the laying hen of the loss that therefore disease is brought to raiser.

summary of the invention:

The object of the invention is to overcome the shortcoming existing in prior art, provide a kind of method simple, easy to use, prevention and cure chicken inclusion body hepatitis is effective, can obviously reduce the cultural method of the laying hen of the loss that therefore disease is brought to raiser.

To achieve these goals, the invention provides a kind of manufacture method of Chinese medicine composition of anti-inclusion body hepatitis, wherein, operation in accordance with the following steps:

Broiler during age in week, carries out feeding by following mass percent configuration feedstuff: Semen Maydis 57.4%, Testa Tritici 3%, bean cake 20%, fish flour 2%, rapeseed cake 4%, cottonseed meal 3%, calcium hydrogen phosphate 1%, compound vitamin 0.3%, stone powder 8%, Sal 0.3%, disease-resistant adjuvant 1% at 3-7;

The component of described disease-resistant adjuvant is: Rhizoma Cimicifugae, Herba Artemisiae Annuae, each 90-130g of Herba Sedi, Radix Scutellariae, Fructus Gardeniae, the Radix Paeoniae Alba, Pericarpium Citri Reticulatae Viride, Radix Codonopsis, Fructus Lycii, Folium Artemisiae Argyi, each 50-80g of Galla Chinensis, Radix Notoginseng, Semen Phaseoli, Herba Houttuyniae, Rhizoma Coptidis, Herba Ecliptae, Rhizoma Zingiberis Recens, each 30-60g of Herba Taxilli, Radix Glycyrrhizae 25-50g;

When prepared by disease-resistant adjuvant: after said components is mixed, with water extract method lixiviate three times, after three times lixiviating solution merges, steaming and decocting obtains mixture of viscous form, adds modified starch, hydroxy methocel and sweeting agent; Modified starch, hydroxy methocel and sweeting agent mass ratio are 4:1:0.5, the quality of modified starch, hydroxy methocel and sweeting agent and be the 1-1.5% of mixture of viscous form quality, after stirring, being dried, obtain powder, powder is after ultra-vioket radiation is sterilized 10 seconds, add glucose powder and gentamycin sulfate powder, the mass ratio of glucose powder and gentamycin sulfate powder is 3:1, the quality of the glucose powder adding and gentamycin sulfate powder and for powder quality 1%, after mix homogeneously, obtain disease-resistant adjuvant.

Or, the component of disease-resistant adjuvant is: Rhizoma Cimicifugae, Herba Artemisiae Annuae, each 90-110g of Herba Sedi, Radix Scutellariae, Fructus Gardeniae, the Radix Paeoniae Alba, Pericarpium Citri Reticulatae Viride, Radix Codonopsis, Fructus Lycii, Folium Artemisiae Argyi, each 50-70g of Galla Chinensis, Radix Notoginseng, Semen Phaseoli, Herba Houttuyniae, Rhizoma Coptidis, Herba Ecliptae, Rhizoma Zingiberis Recens, each 30-50g of Herba Taxilli, Radix Glycyrrhizae 25-40g.

Or, the component of disease-resistant adjuvant is: Rhizoma Cimicifugae, Herba Artemisiae Annuae, each 110-130g of Herba Sedi, Radix Scutellariae, Fructus Gardeniae, the Radix Paeoniae Alba, Pericarpium Citri Reticulatae Viride, Radix Codonopsis, Fructus Lycii, Folium Artemisiae Argyi, each 70-80g of Galla Chinensis, Radix Notoginseng, Semen Phaseoli, Herba Houttuyniae, Rhizoma Coptidis, Herba Ecliptae, Rhizoma Zingiberis Recens, each 50-60g of Herba Taxilli, Radix Glycyrrhizae 40-50g.

Or, the component of disease-resistant adjuvant is: Rhizoma Cimicifugae, Herba Artemisiae Annuae, each 95-125g of Herba Sedi, Radix Scutellariae, Fructus Gardeniae, the Radix Paeoniae Alba, Pericarpium Citri Reticulatae Viride, Radix Codonopsis, Fructus Lycii, Folium Artemisiae Argyi, each 55-75g of Galla Chinensis, Radix Notoginseng, Semen Phaseoli, Herba Houttuyniae, Rhizoma Coptidis, Herba Ecliptae, Rhizoma Zingiberis Recens, each 35-55g of Herba Taxilli, Radix Glycyrrhizae 30-45g.

Further, in the feedstuff of configuration, add vitamin K3, the amount adding is that feedstuff per ton is no more than 5g.(optional)

Vitamin k 3, Chinese name, menadione; Menadione sodium bisulfite; 2-MNQ; Vitamin K3 is the essential material of the synthetic protoenzyme (factor B) of liver, and participates in proconvertin, and IX, and the synthesizing of X, maintain the blood coagulation physiological process of animal.The K that is deficient in vitamin can cause above-mentioned thrombin dyssynthesis, affects coagulation process and causes bleeding.It is essential that vitamin K also relies on carboxylation system by the vitamin K that (mainly refers to be present in the histoorgans such as liver, skeleton, testis, skin and kidney) in animal body, is the indispensable factor in skeleton element (BGP) building-up process.In addition, in energy-rich compound metabolism and oxidative phosphorylation process, and all play an important role with the aspect of other fatsoluble vitamin metabolism, and there is diuresis, enhancing liver detoxification function, participate in the structure of film, the function reducing blood pressure.

Disease-resistant adjuvant of the present invention, the preparation method of employing Chinese patent medicine, the Rhizoma Cimicifugae of the present invention to detoxify, antitoxin Herba Artemisiae Annuae, the Herba Sedi of jaundice eliminating damp eliminating is monarch drug.With liver heat removing hemostasis to medicine Radix Scutellariae, Fructus Gardeniae, soothing the liver circulation of qi promoting to the medicine Radix Paeoniae Alba, Pericarpium Citri Reticulatae Viride, increase hematopoietic potential, increase medicine Radix Codonopsis, the Fructus Lycii of erythrocyte and hemoglobin, Folium Artemisiae Argyi, the Galla Chinensis with hemostatic function are ministerial drug.With Radix Notoginseng, the Semen Phaseoli of increased platelets counts content, increase Herba Houttuyniae, the Rhizoma Coptidis of leukocytes phagocytic ability, the Herba Ecliptae of removing toxic substances, Rhizoma Zingiberis Recens, antitoxin Herba Taxilli is adjuvant drug.The Radix Glycyrrhizae of usining is in harmonious proportion the property of medicine as making medicine.Meanwhile, use modified starch, hydroxy methocel and sweeting agent seasoning, used glucose powder and gentamycin sulfate powder (for animals) to increase effect.

Treat the sick chicken of the inclusion body hepatitis of having fallen ill, disease-resistant adjuvant is added to water modulation quality than 8-12%, every gavages 5-10ml, and 2 times on the one, gavage continuously 3, effect is remarkable.

Advantage of the present invention is: method is simple, easy to use, and prevention and cure chicken inclusion body hepatitis is effective, can obviously reduce the loss that therefore disease is brought to raiser.Be specially, in the high period of disease of broiler, disease-resistant adjuvant mixed in feedstuff and carries out feeding, increase resistance against diseases.For the sick chicken of ill inclusion body hepatitis, disease-resistant adjuvant bath is gavaged and treated.

the specific embodiment:

The manufacture method of the Chinese medicine composition of anti-inclusion body hepatitis, wherein, operation in accordance with the following steps:

Example 1:

The Jimo Duan Bolan town raiser of grandson family:

Broiler during age in week, carries out feeding by following mass percent configuration feedstuff: Semen Maydis 57.4%, Testa Tritici 3%, bean cake 20%, fish flour 2%, rapeseed cake 4%, cottonseed meal 3%, calcium hydrogen phosphate 1%, compound vitamin 0.3%, stone powder 8%, Sal 0.3%, disease-resistant adjuvant 1% at 3-7; After mixing feedstuff feeding on the one 3-4 time.

The component of described disease-resistant adjuvant is: Rhizoma Cimicifugae, Herba Artemisiae Annuae, each 130g of Herba Sedi, Radix Scutellariae, Fructus Gardeniae, the Radix Paeoniae Alba, Pericarpium Citri Reticulatae Viride, Radix Codonopsis, Fructus Lycii, Folium Artemisiae Argyi, each 80g of Galla Chinensis, Radix Notoginseng, Semen Phaseoli, Herba Houttuyniae, Rhizoma Coptidis, Herba Ecliptae, Rhizoma Zingiberis Recens, each 60g of Herba Taxilli, Radix Glycyrrhizae 50g;

For the sick chicken of the inclusion body hepatitis of falling ill, after morbidity, use disease-resistant adjuvant to add the solution that water is configured to mass percent 12%, every gavages 10ml.

Example 2

Defense preparations town, Laixi is the raiser of family recklessly:

Broiler during age in week, carries out feeding by following mass percent configuration feedstuff: Semen Maydis 57.4%, Testa Tritici 3%, bean cake 20%, fish flour 2%, rapeseed cake 4%, cottonseed meal 3%, calcium hydrogen phosphate 1%, compound vitamin 0.3%, stone powder 8%, Sal 0.3%, disease-resistant adjuvant 1% at 3-7; After mixing, obtain feedstuff, add vitamin K3, the amount adding is no more than 5g per ton.Feeding was 3-4 time on 1st.

The component of described disease-resistant adjuvant is: Rhizoma Cimicifugae, Herba Artemisiae Annuae, each 120g of Herba Sedi, Radix Scutellariae, Fructus Gardeniae, the Radix Paeoniae Alba, Pericarpium Citri Reticulatae Viride, Radix Codonopsis, Fructus Lycii, Folium Artemisiae Argyi, each 70g of Galla Chinensis, Radix Notoginseng, Semen Phaseoli, Herba Houttuyniae, Rhizoma Coptidis, Herba Ecliptae, Rhizoma Zingiberis Recens, each 50g of Herba Taxilli, Radix Glycyrrhizae 40g;

For the sick chicken of the inclusion body hepatitis of falling ill, after morbidity, use disease-resistant adjuvant to add the solution that water is configured to mass percent 10%, every gavages 10ml.

Example 3

Qingdao Tian Rui animal husbandry Science and Technology Ltd. (the applicant's affiliated enterprise):

The component of described disease-resistant adjuvant is: Rhizoma Cimicifugae, Herba Artemisiae Annuae, each 110g of Herba Sedi, Radix Scutellariae, Fructus Gardeniae, the Radix Paeoniae Alba, Pericarpium Citri Reticulatae Viride, Radix Codonopsis, Fructus Lycii, Folium Artemisiae Argyi, each 60g of Galla Chinensis, Radix Notoginseng, Semen Phaseoli, Herba Houttuyniae, Rhizoma Coptidis, Herba Ecliptae, Rhizoma Zingiberis Recens, each 40g of Herba Taxilli, Radix Glycyrrhizae 35g;

For the sick chicken of the inclusion body hepatitis of falling ill, after morbidity, use disease-resistant adjuvant to add the solution that water is configured to mass percent 8%, every gavages 10ml.

Above-mentioned 3 example list comparisons, (for the sickness rate after the control of inclusion body hepatitis, the effective percentage after treatment).

Example 1-3 respectively select 6 week age broiler each 500, 500 of broiler simultaneously selecting 6 week age from Qingdao Lan Yingqin industry Science and Technology Ltd. as a control group, respectively 10 inputs of sick chicken of suffering from inclusion body hepatitis are raised together with, infect experiment, within 3 days, observe afterwards, then the sick chicken of the inclusion body hepatitis of having fallen ill is treated, matched group is used (2 milliliters every of vitamin C injections, containing 0.1 milligram), vitamin K3, (1 milliliter every of K4 injection, containing 4 milligrams), (1 milliliter every of gentamycin, containing 20 milligrams) each 1, to 250 milliliters of cold water, allow disease chicken freely drink.

By upper table, can find that the application's cultural method can effectively reduce the probability that in breeding process, inclusion body hepatitis is fallen ill.And, can find the aqueous solution of 12% mass ratio of the disease-resistant medicine of the application, therapeutic effect is good.

Contrast experiment's data:

Laboratory animal, chooses through the sick chicken of inclusion body hepatitis and invades then, and 1000 of the broiler in definite 4 week age of catching an illness, are divided into 5 groups at random,

8% solution of 1 group of disease-resistant adjuvant bath configuration of using the embodiment of the present application 1, gavages 5ml every day, every day twice,

2 groups are used prior art Chinese patent application 2013100743847 to disclose a kind of Chinese medicine oral liquid for the treatment of chicken inclusion body hepatitis.

3 groups are used in prior art, Chinese medicine formula: Folium Isatidis 60 g, and Herba Artemisiae Scopariae 30 g, Fructus Gardeniae 45 g, Rhizoma Polygoni Cuspidati 30 g, Radix Et Rhizoma Rhei 20 g, Herba Plantaginis 35 g, every 100 kg feedstuffs mix 0.5～1.0 kg.

4 groups, use Chinese patent application 2010102703573 to disclose a kind of for preventing and treat the Chinese medicine feed additive of chicken liver lesion.

5 groups are used in background technology, the clear spice of sense poison, 3% glucose+vitamin C drinking-water.

Relatively therapeutic effect, respectively organized treatment after 4 days, recorded mortality rate and body weight contrast, continued feeding after 10 days, relatively recovered the time of normal type.Image data is listed as follows

6 groups of healthy broiler that select 4 week age, for comparing the weight recovery time (each group and 6 differs and is less than 10g and calculates the normal value that returns back to 6 groups), 6 groups as Normal group.

By upper table, contrast, can find, the application's cure rate is high, therapeutic effect for inclusion body hepatitis is remarkable, and treatment required time is short, and the time of recovering body weight is fast, mortality rate, lower than other schemes of the prior art, can significantly reduce the therefore sick loss bringing of raiser.

The toxicity test result of the disease-resistant adjuvant of example 3 of the present invention:

The experiment of toxicity to rat:

Acute oral (LD50) > 500mg/kg,

Acute percutaneous (LD50) > 2000mg/kg,

Belong to low toxicity.

Antiviral experimental data:

Get a sick chicken and do not do any treatment, as virus control;

Above-mentioned 1-6 group, every group treatment within the 4th day, randomly draw one, take after body weight fixingly, dissect, get liver and calculate liver exponential quantity, calculate liver index suppression ratio.

Heavy (the g)/body weight (g) * 100 of liver index=liver

Liver index suppression ratio=(virus control group liver Mean value of index-experimental group liver Mean value of index)/virus control group liver Mean value of index * 100%

Liver index is larger, represents that liver pathological changes degree is serious,

Hepatic tissue is fixed with 10% formaldehyde, ethanol series dehydration, dimethylbenzene is transparent, paraffin embedding, slice thickness 4-5 micron, HE dyeing, observes blood lipid with ordinary optical microscope and changes, with the liver of Normal group as a comparison.

The impact (n=10) of liver's checking (virus is observed)

By above-mentioned contrast, component that can 1 group of the application has good suppression ratio to virus.

Claims

1. the manufacture method of the Chinese medicine composition of an anti-inclusion body hepatitis, it is characterized in that, component is: Rhizoma Cimicifugae, Herba Artemisiae Annuae, Herba Sedi, Radix Scutellariae, Fructus Gardeniae, the Radix Paeoniae Alba, Pericarpium Citri Reticulatae Viride, Radix Codonopsis, Fructus Lycii, Folium Artemisiae Argyi, Galla Chinensis, Radix Notoginseng, Semen Phaseoli, Herba Houttuyniae, Rhizoma Coptidis, Herba Ecliptae, Rhizoma Zingiberis Recens, Herba Taxilli, Radix Glycyrrhizae;

After said components is mixed, with water extract method lixiviate three times, after three times lixiviating solution merges, steaming and decocting obtains mixture of viscous form, adds modified starch, hydroxy methocel and sweeting agent; Modified starch, hydroxy methocel and sweeting agent mass ratio are 4:1:0.5, the quality of modified starch, hydroxy methocel and sweeting agent and be the 1-1.5% of mixture of viscous form quality, after stirring, being dried, obtain powder, powder is after ultra-vioket radiation is sterilized 10 seconds, add glucose powder and gentamycin sulfate powder, the mass ratio of glucose powder and gentamycin sulfate powder is 3:1, the quality of the glucose powder adding and gentamycin sulfate powder and for powder quality 1%, after mix homogeneously, obtain disease-resistant adjuvant.