CN104161223A - Composite formula feed for breeding blue crabs - Google Patents

Composite formula feed for breeding blue crabs Download PDF

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Publication number
CN104161223A
CN104161223A CN201410260344.6A CN201410260344A CN104161223A CN 104161223 A CN104161223 A CN 104161223A CN 201410260344 A CN201410260344 A CN 201410260344A CN 104161223 A CN104161223 A CN 104161223A
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powder
composite
feed
vitamin
portunus trituberculatus
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CN104161223B (en
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辛俭
楼宝
史会来
余方平
徐冬冬
詹炜
毛国民
陈睿毅
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Zhejiang Marine Fisheries Research Institute
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Zhejiang Marine Fisheries Research Institute
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The invention relates to a composite formula feed for breeding blue crabs. The raw materials of the feed comprise the following components in percentage by weight: 25-28% of wheat flour, 9-12% of corn DDGS, 15-18% of fly larva powder, 12-14% of albumen powder, 3-5% of beer yeast, 1-2% of DHA micro algae oil, 1% of EPA refined oil, 2% of fish oil, 1% of blue-green algae powder, 0.1-0.2% of choline chloride, 0.2% of sodium chloride, 0.04-0.05% of L-carnitine, 2-4% of isomaltooligosacharide, 0.3% of composite bacillus, 0.05-0.06% of mildewproof agents, 1-3% of compounding adhesives, 1-2% of composite minerals, 0.5-1.5% of composite vitamins, and the balance of biogas residues. Various components are effectively mixed, and fish meal and soybean meal are prevented from being used, so that the influence of nutrition resistant factors in the soybean meal on the growth of the blue crabs is reduced.

Description

For the compound prescription feed of Portunus trituberculatus Miers cultivation
Technical field
The invention belongs to mariculture technical field, particularly a kind of compound prescription feed for Portunus trituberculatus Miers cultivation.
Background technology
Fish meal as the major protein source of aquatic feeds because its protein content is high, amino acid Compositional balance, palatability is good, ANFs is few, is widely used in culture fishery always.Global aquaculture scale is just with annual 11% speed increment, but the annual production of global fish meal declines increasingly, and the annual production of fish meal in recent years only maintains 600~7,000,000 t and caused the unevenness between supply and demand of high-quality feed protein sources.Therefore fish meal alternative materials, the research of finding high-quality substitutes the adding proportion of protein sources has become the key issue that can culture fishery sustainable development.Dregs of beans, because having the advantages such as high protein content, reasonable price, steady sources, amino acid pattern ideal, is carried out research as high-quality fish meal substitute always widely.But its palatability is poor, the ANFs in dregs of beans can reduce its speed of growth by the digestibility and the protein utilization rate that reduce fish.Therefore Peru Fish Dietary in a large number still at present.
Portunus trituberculatus Miers, is commonly called as swimming crab, Blue Swimming Crab, belongs to Crustachia, Decapoda, Portumidae, is the Important Economic crab class of coastal area of china.Portunus trituberculatus Miers is dwelt under the ooze of 7~100 meters of the offshore depth of waters, sand mud bedstone or in pasture and water.For adapting to market demand and suitably reserved resource, coastal some places are carried out and are propagated artificially in recent years.It is introduced, cultivation is taking Tu Chi as good, and pond structure is similar with intensive culture prawn soil pond, and area is advisable with middle-size and small-size, 1.5 meters~2 meters of the depth of waters, and keep good water quality, and at the bottom of pond, lay coarse sand and sheltered situation, allow it perch.Tradition cultivation is thrown something and fed and is frozen mixed feed that trash fish, shrimp, nutrition composition and food calling effect do not conform to Portunus trituberculatus Miers as bait; Cause pond culture survival rate low, only 4%-8%, has a strong impact on cultured output and raiser's income.
Summary of the invention
The invention provides and a kind ofly improve Portunus trituberculatus Miers survival rate, meet Growth of Portunus Trituberculatus needs and avoid adopting the Pelletized Feed For Swimming Crab Portunus Trituberculatus of fish meal as raw material.
The technical solution adopted for the present invention to solve the technical problems is:
For a compound prescription feed for Portunus trituberculatus Miers cultivation, its raw material comprises the component in weight percentage as follows: wheat flour 25-28%, corn DDGS 9-12%, fly maggot powder 15-18%, albumen powder 12-14%, the micro-algae oil of brewer's yeast 3-5%, DHA 1-2%, EPA refined oil 1%, fish oil 2%, blue algae powder 1%, Choline Chloride 0.1-0.2%, sodium chloride 0.2%, l-cn 0.04-0.05%, isomalto-oligosaccharide 2-4%, composite bacillus 0.3%, mould inhibitor 0.05-0.06%; Composite adhesive 1-3%, composite mineral matter 1-2%, B B-complex 0.5-1.5%;
Surplus is natural pond slag, and described natural pond slag is the vinasse products therefrom after anaerobic fermentation taking cassava or Ipomoea batatas as fermentation raw material, after adopting mechanical presses to dewater, tans by the sun to dry, after then pulverizing with pulverizer, uses.Control formula diet moisture≤12.5%, each component is crossed 80 mesh standard sieves after mixing.
Described albumen powder is mixed with arbitrary proportion by one or more in soyabean protein powder, rice protein powder or corn protein powder.
Described in every 1000g, in B B-complex, contain vitamin A 10000000-15000000IU, vitamin D 32000000-3000000IU, vitamin E 80000-120000IU, vitamin K 36000-12000mg, Cobastab 110000-30000mg, Cobastab 220-30mg, nicotinic acid 60000-80000mg, calcium pantothenate 20000-30000mg, folic acid 2000-3000mg, biotin 200-300mg, inositol 80000mg-120000mg and vitamin C 150000-250000mg, all the other are wheat bran;
Described in every 1000g, in composite adhesive, contain Gluten 250-350g, algin 350-450g, tamarind gum 150-250g and dextrin 50-150g;
Described in every 1000g in composite mineral matter, contain iron 20000-30000mg, manganese 3000-5000mg, copper 1500-2500mg, zinc 6000-8000mg, cobalt 500-700mg, iodine 3000-4000mg, selenium 100-150mg, magnesium 50000-60000mg and potassium 25000-35000mg, all the other are medical stone.
This formula feed also comprises the component in weight percentage as follows: hawthorn 3%, binchotan powder 1%, wherein the particle diameter of binchotan powder is 3000 orders.Adding of hawthorn, can significantly improve the rate of body weight gain (P < 0.01) of Portunus trituberculatus Miers and reduce feed coefficient (P < 0.05).Binchotan powder is as a kind of special additive, can be used as on the one hand adsorbent uses, harmful substance and issuable mould etc. in absorption formula feed, can reduce on the other hand the poisonous and harmful substances in Portunus trituberculatus Miers body, reduce the injury of environment to Portunus trituberculatus Miers, thereby improve its survival rate.
The present invention effectively mixes various ingredients, reduce the impact of ANFs on Growth of Portunus Trituberculatus in dregs of beans, suitable blue algae powder can increase the agreeable to the taste degree of Portunus trituberculatus Miers, the protein of feed of the present invention and fat content all meet the growth of Portunus trituberculatus Miers, and fly maggot powder, composite bacillus and the isomalto-oligosaccharide etc. of increase effectively reduce Portunus trituberculatus Miers and infect ill risk.Mixed feed of the present invention can mix input according to the addition of 40-60% together with conventional feed.
The interpolation of composite bacillus significantly affects stomach, trypsase and the hepatopancrease lipase active of Portunus trituberculatus Miers, but, excessive interpolation bacillus can significantly reduce trypsase and the lipase active of test Portunus trituberculatus Miers, and this may be to have destroyed the colony balance in its body because add too much bacillus; And bacillus produces in vivo excessive enzyme and may impact the secretion of the active of body endogenous enzyme or inhibition endogenous enzyme.Therefore, the addition of composite bacillus needs careful control.Isomalto-oligosaccharide not only can promote the beneficial bacterium in aquatic products gut flora---the propagation of Bifidobacterium, can suppress harmful bacteria simultaneously---the growth of C.perfringens, and effect is extremely remarkable, makes gut flora maintain positive balance state.Isomalto-oligosaccharide jointly regulates the gut flora of Portunus trituberculatus Miers together with composite bacillus, improves its digestive efficiency.
Bacillus can produce the volatile fatty acids such as lactic acid and acetic acid, propionic acid, butyric acid in enteron aisle in growth and breeding process, reduce enteron aisle pH value, harmful microorganism is suppressed, regulate alimentary canal microecological balance, and the reduction of enteron aisle pH value can promote the digestion of the aquatic livestock young to nutriment, the especially digestion to protein and absorption.On the other hand, bacillus subtilis is in its growth, in reproductive process, again can self synthetic VC, VB and vitamin and the coenzyme thereof such as VK, and the nutriment such as organic acid and amino acid, can be animal nutrition is provided, promote its growth.In addition, after probiotics field planting in Host Digestion road, procreation, metabolism, some digestive enzyme activity materials of generation, can promote digestion and the absorption of digestive system to feed nutrient, improve digestive efficiency.
The micro-algae oil of DHA extracts from marine microalgae, composition safety.The micro-algae oil of DHA of appropriate proportioning and EPA refined oil, for the growth of crab class provides essential aliphatic acid, are conducive to improve survival rate and the speed of growth of young crab, if but too high levels, can deleterious.The effect that the micro-algae oil of DHA and EPA refined oil mix regulation and control is than adopting separately a kind of better effects if.
Natural pond slag is organic waste anaerobic fermentation product after treatment, it is a kind of valuable resource, it contains abundant soluble inorganic salt class nutriment and the multiple biochemical product (as: trace element such as nitrogen, phosphorus, potassium, boron, copper, manganese, calcium, zinc, and several amino acids), easily absorbed by biological, crop.In addition, its protein content is 23.5%-35.8%, and cobalamin is 17.6mg/kg (dry weight), can be used as after treatment feed, and nutrition, economic worth are considerable.
Contained nutrient in the slag of natural pond, is the necessary nutrient of crop growth, is also the Major Nutrient material in livestock and poultry, aquaculture feed.Therefore, the recycling of natural pond slag has much value.Make full use of natural pond slag, not only can alleviate to a certain extent crisis of resource, turn waste into wealth, also can, reducing under the prerequisite of ambient pollution burden, bring considerable economic benefit and social benefit.In recent years, some large-scale breweries, Alcohol Plant (as Sichuan Mianzhu and Linyi, Shandong Deng Di producer) build anaerobic fermentation facility energetically, actively use biogas anaerobic fermentation technology, built a collection of large-sized biogas engineering taking vinasse as raw material, natural pond slag output is considerable.But these tunnings do not obtain rationally, effectively utilize, and have formed the waste of resource.At present, whole feed resource supply-demand relationship presents the features such as concentrated feed lacks, protein feeds lacks, green feed lacks, insufficient total amount, and natural pond slag is not used widely in field of fodder, urgently development and utilization.In the present invention, reasonably utilize discarded object, turned waste into wealth, realized resource recycling.
Corn DDGS, is the trade name of protein feed in vinasse, contains the distiller's dried grain of soluble solids.Taking corn in fermenting raw materials ethanol production process, starch is wherein converted to ethanol and carbon dioxide, other nutritional labelings are all stayed in vinasse as protein, fat, fiber etc.Due to the effect of microorganism, in vinasse, protein, B family vitamin and amino acid content all increase to some extent than corn, and contain the unknown somatomedin generating in fermentation simultaneously.The each component of the relatively existing aquatic products feed of corn DDGS, originates wide, relative low price, and owing to processing by fermentation, therefore composition wherein easily absorbs.DDGS moisture is high, and cereal is damaged, and mould easily grows, and therefore mycotoxin content is very high, may have multiple mycotoxin, can cause the Mycotoxicoses of domestic animal.Cause immunocompromised susceptible disease, production performance declines.So must be with mould inhibitor or wide spectrum mold toxin sorbent.After careful composite of the present invention, having formed can be for the mixed feed of Growth of Portunus Trituberculatus.
The invention has the beneficial effects as follows: the present invention effectively mixes various ingredients, avoided the use of fish meal and dregs of beans, reduced the impact of ANFs on Growth of Portunus Trituberculatus in dregs of beans.Reasonable component formula of the present invention, sufficient raw, raw material components is nutritious, has greatly improved recycling rate, has improved significantly survival rate and the rate of body weight gain of Portunus trituberculatus Miers simultaneously.
Detailed description of the invention
Below by specific embodiment, technical scheme of the present invention is described in further detail.Should be appreciated that enforcement of the present invention is not limited to the following examples, any pro forma accommodation that the present invention is made and/or change all will fall into protection domain of the present invention.
In the present invention, if not refer in particular to, all part, percentages are unit of weight, and all equipment and raw materials etc. all can be buied from market or the industry is conventional.
Composite bacillus is provided by Zhejiang Microbe Inst., is mainly bafillus natto (Bacillus subtilis Natto) and bacillus subtilis (Bacillus subtilis Cohn), and bacterium liquid bacteria containing amount is 1 × 10 10cfug -1.
The micro-algae oil of DHA (DHA content is that 40.00%, EPA content is 2.44%), is provided by Jiangsu Tiankai Biotechnology Co., Ltd.; EPA refined oil (EPA content is that 45%, DHA content is 25%), healthy bio tech ltd, source, the effluent North Sea provides.
Described in every 1000g, in B B-complex, contain retinol1 0000000-15000000IU, vitamin D 32000000-3000000IU, vitamin E 80000-120000IU, vitamin K 36000-12000mg, Cobastab 110000-30000mg, Cobastab 220-30mg, nicotinic acid 60000-80000mg, calcium pantothenate 20000-30000mg, folic acid 2000-3000mg, biotin 200-300mg, inositol 80000mg-120000mg and vitamin C 150000-250000mg, all the other are wheat bran;
Described in every 1000g, in composite adhesive, contain Gluten 250-350g, algin 350-450g, tamarind gum 150-250g and dextrin 50-150g;
Described in every 1000g in composite mineral matter, contain iron 20000-30000mg, manganese 3000-5000mg, copper 1500-2500mg, zinc 6000-8000mg, cobalt 500-700mg, iodine 3000-4000mg, selenium 100-150mg, magnesium 50000-60000mg and potassium 25000-35000mg, all the other are medical stone.
The source of natural pond slag: operation, all normal large-scale anaerobic fermentation equipments of aerogenesis taking vinasse as raw material, natural pond liquid pH value is within the scope of 6.5-7.5, the natural pond slag that becomes thoroughly decomposed of feed intake every day, discharging every day, go out most of moisture by mechanical presses mode, uniform spreading is to thickness 5-10cm, tan by the sun to dry, then pulverize with pulverizer, for subsequent use.After testing, natural pond slag ingredient is in table 1.
Table 1 natural pond slag ingredient table
Embodiment 1-3:
A kind of Pelletized Feed For Swimming Crab Portunus Trituberculatus, its raw material is in table 2.
Table 2
Above-mentioned each component is crossed 80 mesh standard sieves after mixing, and then adds water to make soft graininess, controls formula diet moisture≤12.5%.
In embodiment 1,
The composition of B B-complex is: in every 1000g B B-complex, contain vitamin A 12000000IU, vitamin D 32500000IU, vitamin E 100000IU, vitamin K 39000mg, Cobastab 120000mg, Cobastab 225mg, nicotinic acid 70000mg, calcium pantothenate 25000mg, folic acid 2500mg, biotin 250mg, inositol 100000mg, Catergen 00000mg, all the other are wheat bran.The composition of composite adhesive is: the composite adhesive of every 1000g contains Gluten 300g, algin 400g, tamarind gum 200g, dextrin 100g.The composition of composite mineral matter is: every 1000g composite mineral matter contains iron 25000mg, manganese 4000mg, copper 2000mg, zinc 7000mg, Co 60 0mg, iodine 3500mg, selenium 125mg, magnesium 55000mg, potassium 30000mg, and all the other are medical stone.
In embodiment 2,
The composition of B B-complex is: every 1000g B B-complex contains vitamin A 10000000IU, vitamin D 32000000IU, vitamin E 80000IU, vitamin K 36000mg, Cobastab 110000mg, Cobastab 220mg, nicotinic acid 60000mg, calcium pantothenate 20000mg, folic acid 2000mg, biotin 200mg, inositol 80000mg, vitamin C 150000mg, all the other are wheat bran.The composition of composite adhesive is: the composite adhesive of every 1000g contains Gluten 250g, algin 350g, tamarind gum 250g, dextrin 150g.The composition of composite mineral matter is: every 1000g is multiple, and composite mineral matter contains iron 20000mg, manganese 3000mg, copper 1500mg, zinc 6000mg, cobalt 500mg, iodine 3000mg, selenium 100mg, magnesium 50000mg, potassium 25000mg, and all the other are medical stone.
In embodiment 3,
The every 1000g of B B-complex contains vitamin A 15000000IU, vitamin D 33000000IU, vitamin E 120000IU, vitamin K 312000mg, Cobastab 130000mg, Cobastab 230mg, nicotinic acid 80000mg, calcium pantothenate 30000mg, folic acid 3000mg, biotin 300mg, inositol 120000mg, Catergen 50000mg, all the other are wheat bran.The composition of composite adhesive is: the composite adhesive of every 1000g contains Gluten 350g, algin 450g, tamarind gum 150g, dextrin 50g.The composition of composite mineral matter is: every 1000g composite mineral matter contains iron 30000mg, manganese 5000mg, copper 2500mg, zinc 8000mg, cobalt 700mg, iodine 4000mg, selenium 150mg, magnesium 60000mg, potassium 35000mg, and all the other are medical stone.
Use the feed of the present embodiment to raise swimming crab, cultivation survival rate reaches 16.8%, has relatively improved 110% with 8% survival rate of traditional cultivation method.
Comparative example
Feed formula: imported fish meal 28kg, flour 22.205kg, 46% dregs of beans 16.5kg (described percentage is the mass percent that the protein in dregs of beans accounts for dregs of beans), corn protein powder 3kg, peanut meal 12.5kg, cotton dregs 3.8kg, brewer's yeast 2kg, cuttlefish cream 2kg, shrimp shell meal 2kg, calcium dihydrogen phosphate 1kg, phosphatide oil 1.5kg, fish oil 1.2kg, trace element 0.5kg, betaine 0.2kg, salt 0.2kg, multidimensional 0.175kg, Choline Chloride 0.1kg, mould inhibitor 0.05kg, l-cn 0.05kg, zeolite powder 3kg, ethoxyquinoline 0.02kg.
Culture experiment
Select 300 of Portunus trituberculatus Miers seedlings, adopt comparative example feed to tame, the domestication time is 10d, adapts to breeding environment completely at Portunus trituberculatus Miers, ingests active, and enters formal test in the situation that does not have to die unexpectedly.Be divided at random 4 groups, every group of 3 repetitions.The DIFFERENT FEED of embodiment 1-3 and the comparative example 1 of feeding respectively.Test site is carried out in cultivation base, Zhoushan, Zhejiang Province.Test period 160 days.
Table 3
From table 3, embodiment not containing the feed of fish meal at feed coefficient, survival rate, specific growth rate all has good performance than comparative example, especially survival rate aspect tool increases significantly.Embodiment feed can replace traditional high fish-meal completely.Observe through histotomy, liver and enteron aisle are all without obvious alteration of form.
The high meal compound feed of mixed feed energy replacing whole tradition provided by the invention, Growth of Portunus Trituberculatus, survival rate etc. are had no adverse effects, and cost is low, culture benefit is high, reduce the dependence to high-quality fish meal, for the sustainable development of Portunus trituberculatus Miers cultivation provides a kind of good demonstration and reference function.
Above-described embodiment is preferably scheme of one of the present invention, not the present invention is done to any pro forma restriction, also has other variant and remodeling under the prerequisite that does not exceed the technical scheme that claim records.

Claims (4)

1. for a compound prescription feed for Portunus trituberculatus Miers cultivation, it is characterized in that its raw material comprises the component in weight percentage as follows:
Wheat flour 25-28%, corn DDGS 9-12%, fly maggot powder 15-18%, albumen powder 12-14%, the micro-algae oil of brewer's yeast 3-5%, DHA 1-2%, EPA refined oil 1%, fish oil 2%, blue algae powder 1%, Choline Chloride 0.1-0.2%, sodium chloride 0.2%, l-cn 0.04-0.05%,
Isomalto-oligosaccharide 2-4%, composite bacillus 0.3%, mould inhibitor 0.05-0.06%; Composite adhesive 1-3%, composite mineral matter 1-2%, B B-complex 0.5-1.5%;
Surplus is natural pond slag, and described natural pond slag is the vinasse products therefrom after anaerobic fermentation taking cassava or Ipomoea batatas as fermentation raw material, after adopting mechanical presses to dewater, tans by the sun to dry, after then pulverizing with pulverizer, uses.
2. the compound prescription feed for Portunus trituberculatus Miers cultivation according to claim 1, is characterized in that: described albumen powder is mixed with arbitrary proportion by one or more in soyabean protein powder, rice protein powder or corn protein powder.
3. the compound prescription feed for Portunus trituberculatus Miers cultivation according to claim 1 and 2, is characterized in that described in every 1000g, in B B-complex, containing vitamin A 10000000-15000000IU, vitamin D 32000000-3000000IU, vitamin E 80000-120000IU, vitamin K 36000-12000mg, Cobastab 110000-30000mg, Cobastab 220-30mg, nicotinic acid 60000-80000mg, calcium pantothenate 20000-30000mg, folic acid 2000-3000mg, biotin 200-300mg, inositol 80000mg-120000mg and vitamin C 150000-250000mg, all the other are wheat bran;
Described in every 1000g, in composite adhesive, contain Gluten 250-350g, algin 350-450g, tamarind gum 150-250g and dextrin 50-150g;
Described in every 1000g in composite mineral matter, contain iron 20000-30000mg, manganese 3000-5000mg, copper 1500-2500mg, zinc 6000-8000mg, cobalt 500-700mg, iodine 3000-4000mg, selenium 100-150mg, magnesium 50000-60000mg and potassium 25000-35000mg, all the other are medical stone.
4. the compound prescription feed for Portunus trituberculatus Miers cultivation according to claim 1 and 2, is characterized in that this formula feed also comprises the component in weight percentage as follows: hawthorn 3%, binchotan powder 1%, wherein the particle diameter of binchotan powder is 3000 orders.
CN201410260344.6A 2014-06-12 2014-06-12 For the compound prescription feed of Portunus trituberculatus Miers cultivation Active CN104161223B (en)

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CN105410344A (en) * 2015-12-17 2016-03-23 宁波大学 Mixed feed for promoting portunus trituberculatus ovarian development and vitellogenesis and preparation method of mixed feed
FR3037482A1 (en) * 2015-06-19 2016-12-23 Cathy Lopez APPAT OR FOOD FOR INSECT FISH

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Cited By (3)

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Publication number Priority date Publication date Assignee Title
FR3037482A1 (en) * 2015-06-19 2016-12-23 Cathy Lopez APPAT OR FOOD FOR INSECT FISH
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CN105410344A (en) * 2015-12-17 2016-03-23 宁波大学 Mixed feed for promoting portunus trituberculatus ovarian development and vitellogenesis and preparation method of mixed feed

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