CN105104739A - Nushan lake hairy crab breeding feed for improving meat and nutrition - Google Patents
Nushan lake hairy crab breeding feed for improving meat and nutrition Download PDFInfo
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Abstract
The invention provides Nushan lake hairy crab breeding feed for improving meat and nutrition. The breeding feed comprises choline chloride, edible salt, calcium hydrogen phosphate, black carp powder, corn starch, tubificidae powder, compound vitamin additives, medical stone powder, compound mineral substance additives, phytase, chitosan and Chinese herb feed additives. The Nushan lake hairy crab breeding feed containing the Chinese herb feed additives can be utilized, the combination of Chinese herbs, the compound vitamin additives, the compound mineral substance additives and the phytase can be utilized for adjusting nutrients inside hairy crabs and improving meat quality and mouth feeling, and meanwhile, the immunity of the Nushan lake hairy crabs can be effectively enhanced.
Description
Technical field
The present invention relates to field of feed, particularly relate to a kind of lake, female mountain steamed crab breeding feed improving meat nutrition.
Background technology
Aquaculture is one of whole world agricultural with fastest developing speed, the development of high-efficiency feed.
Lake, female mountain steamed crab is Mingguang, anhui special product, this steamed crab is nutritious, crab meat taste saltiness is trembled with fear, and has the merit of heat-clearing, stagnation resolvation, enriching yin, can treat traumatic injury, injury of tendon and muscle fracture, allergic dermatitis, crab shell cineration, adjust with honey, external application can be harnessed the Yellow River bee sting or other nameless sores or boils, and crab meat is also children's natural nourishing product, often eat and can supplement high-quality protein and various trace element, also containing multivitamin in crab meat.At present, along with agalactous universal, be all be main feeding method with feed, because the nutrition in batching is containing quantity not sufficient, the comparision contents of various albumen and vitamin is low, easily cause the protein of steamed crab,
The shortage of fat, carbohydrate, mineral matter and vitamin powder and trace element, steamed crab, due to the deficiency of various nutrition, easily causes steamed crab to grow slowly, the feature of the various illness of little, the easy trouble of head.
For reaching the meat nutrition strengthening lake, female mountain steamed crab, also to reach the object strengthening immunity function, improve lake, female mountain steamed crab premunition simultaneously, the immunologic mechanism of research lake, female mountain steamed crab, carrying out the research and development of immunostimulant, is one of effective means performing organic aquaculture standard.Existing research shows, crustacean is the same with other invertebrate, and lack the specific immune function that vertebrate has, its immune response depends on nonspecific immunity mechanism.
The interpolation of Chinese herbal medicine can adjust the nutritional labeling in crab body, improves meat and mouthfeel.Meanwhile, the disease-prevention health effect of Chinese medicine is obvious, and reason is the composition containing enhancing immunologic function in Chinese medicine material, effectively can regulate non-specific antimicrobial antiviral agent in animal body.Therefore, Chinese herbal medicine can be adopted to accompany bait to throw something and feed to start and regulate the nospecific immunity of lake, female mountain steamed crab, improve himself immunocompetence.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of lake, female mountain steamed crab breeding feed improving meat nutrition, it contains Chinese herb feed additive composition, Chinese herbal medicine can be utilized effectively to strengthen the nutritional labeling of lake, female mountain steamed crab, improve meat and mouthfeel, can also immunocompetence be improved simultaneously.
For solving the problems of the technologies described above, the invention provides a kind of lake, female mountain steamed crab breeding feed improving meat nutrition, comprising: Choline Chloride, salt, calcium monohydrogen phosphate, black carp powder, cornstarch, water earthworm powder, compound vitamin additive, medical stone powder, composite mineral matter additive, phytase, chitosan powder and Chinese herbal feed additive;
The weight portion of each raw material is respectively Choline Chloride 0.5 ~ 1.5 part, salt 3 ~ 5 parts, calcium monohydrogen phosphate 1 ~ 3 part, 30 ~ 40 parts, black carp powder, cornstarch 5 ~ 15 parts, water earthworm powder 5 ~ 10 parts, compound vitamin additive 0.5 ~ 1.5 part, medical stone powder 3 ~ 5 parts, composite mineral matter additive 0.3 ~ 0.6 part, phytase 0.03 ~ 0.1 part, 1 ~ 3 part, chitosan powder, Chinese herbal feed additive 1 ~ 3 part.
Wherein, in lake, the female mountain steamed crab breeding feed of described raising meat nutrition, the weight portion of each raw material is respectively Choline Chloride 1 part, salt 4 parts, calcium monohydrogen phosphate 2 parts, 35 parts, black carp powder, cornstarch 10 parts, water earthworm powder 8 parts, compound vitamin additive 1 part, medical stone powder 4 parts, composite mineral matter additive 0.5 part, phytase 0.05 part, 2 parts, chitosan powder, Chinese herbal feed additive 2 parts.
Wherein, the bulk drug of described Chinese herbal feed additive comprise king taro, golden cypress, adenophora tetraphylla, saline cistanche, hawthorn, the coptis, ti, ajuga decumbens, Asiatic plantain, clarke boea herb, prolong grass, franzenbach rhubarb root and rhizome, Radix Angelicae Sinensis, white slurry rattan and Radix Glycyrrhizae;
In described Chinese herbal feed additive the weight portion of each bulk drug be respectively king taro 25 ~ 35 parts, golden cypress 20 ~ 30 parts, adenophora tetraphylla 15 ~ 25 parts, saline cistanche 15 ~ 25 parts, hawthorn 10 ~ 20 parts, the coptis 10 ~ 20 parts, ti 5 ~ 15 parts, ajuga decumbens 5 ~ 15 parts, Asiatic plantain 5 ~ 15 parts, clarke boea herb 10 ~ 20 parts, prolong grass 15 ~ 25 parts, franzenbach rhubarb root and rhizome 5 ~ 15 parts, Radix Angelicae Sinensis 15 ~ 25 parts, white slurry 10 ~ 20 parts, rattan and 5 ~ 15 parts, Radix Glycyrrhizae.
Wherein, in described Chinese herbal feed additive the weight portion of each bulk drug be respectively king taro 28 ~ 32 parts, golden cypress 23 ~ 27 parts, adenophora tetraphylla 18 ~ 22 parts, saline cistanche 18 ~ 22 parts, hawthorn 13 ~ 17 parts, the coptis 13 ~ 17 parts, ti 8 ~ 12 parts, ajuga decumbens 8 ~ 12 parts, Asiatic plantain 8 ~ 12 parts, clarke boea herb 13 ~ 17 parts, prolong grass 18 ~ 22 parts, franzenbach rhubarb root and rhizome 8 ~ 12 parts, Radix Angelicae Sinensis 18 ~ 22 parts, white slurry 13 ~ 17 parts, rattan and 8 ~ 12 parts, Radix Glycyrrhizae.
Wherein, in described Chinese herbal feed additive the weight portion of each bulk drug be respectively king taro 30 parts, golden cypress 25 parts, adenophora tetraphylla 20 parts, saline cistanche 20 parts, hawthorn 15 parts, the coptis 15 parts, ti 10 parts, ajuga decumbens 10 parts, Asiatic plantain 10 parts, clarke boea herb 15 parts, prolong grass 20 parts, franzenbach rhubarb root and rhizome 10 parts, Radix Angelicae Sinensis 20 parts, white slurry 15 parts, rattan and 10 parts, Radix Glycyrrhizae.
Present invention also offers the preparation method of said herbal medicine feed addictive, be specially:
Take each bulk drug according to described weight portion in grams, pulverize, mix, obtain mixture, decoct every 500 grams of mixtures first and add cold water 800-1000 milliliter, by whole raw material soaking after 30 points to 60 minutes, 30 minutes at a simmer, leach concoction; Add cold water 500-800 milliliter slow fire again and decoct 20-25 minute again, leach concoction for twice and mix even, concentrated removal moisture, dry, pulverize subsequently, obtain Chinese herbal feed additive.
Present invention also offers the preparation method of lake, the female mountain steamed crab breeding feed of above-mentioned raising meat nutrition, it comprises:
The first step, takes black carp powder, medical stone powder, chitosan powder, cornstarch in proportion, mixes rear pulverizing, crosses 45-55 mesh sieve, obtains crushed material;
Second step, take Choline Chloride, salt, calcium monohydrogen phosphate, water earthworm powder, compound vitamin additive, composite mineral matter additive, phytase, Chinese herbal feed additive in proportion, crushed material obtained by itself and the first step is mixed to evenly, obtains raw mixture;
3rd step, carries out modifier treatment by the raw mixture obtained by second step, and refining temperature is 60-68 DEG C, and the modified humidity to raw mixture is 13-15%;
4th step, sends the raw mixture after the 3rd step matter into feed-processing equipment and is processed into granular mixed feed;
5th step, the granular mixed feed of the 4th step gained is dry under 80 DEG C of conditions, be packaged into bag after being then cooled to room temperature.
Beneficial effect of the present invention:
Lake, the female mountain steamed crab breeding feed of the raising meat nutrition containing Chinese herbal feed additive provided by the invention can utilize Chinese herbal medicine to coordinate compound vitamin additive, composite mineral matter additive and phytase can adjust nutritional labeling in crab body, improve meat and mouthfeel, effectively can also strengthen the immunocompetence of lake, female mountain steamed crab simultaneously.
Detailed description of the invention
The invention provides a kind of lake, female mountain steamed crab breeding feed improving meat nutrition, comprising: Choline Chloride, salt, calcium monohydrogen phosphate, black carp powder, cornstarch, water earthworm powder, compound vitamin additive, medical stone powder, composite mineral matter additive, phytase, chitosan powder and Chinese herbal feed additive;
The weight portion of each raw material is respectively Choline Chloride 0.5 ~ 1.5 part, salt 3 ~ 5 parts, calcium monohydrogen phosphate 1 ~ 3 part, 30 ~ 40 parts, black carp powder, cornstarch 5 ~ 15 parts, water earthworm powder 5 ~ 10 parts, compound vitamin additive 0.5 ~ 1.5 part, medical stone powder 3 ~ 5 parts, composite mineral matter additive 0.3 ~ 0.6 part, phytase 0.03 ~ 0.1 part, 1 ~ 3 part, chitosan powder, Chinese herbal feed additive 1 ~ 3 part.
Further preferably, lake, the female mountain steamed crab breeding feed of described raising meat nutrition is only prepared from by above-mentioned raw materials.
Further preferably, in lake, the female mountain steamed crab breeding feed of described raising meat nutrition, the weight portion of each raw material is respectively Choline Chloride 1 part, salt 4 parts, calcium monohydrogen phosphate 2 parts, 35 parts, black carp powder, cornstarch 10 parts, water earthworm powder 8 parts, compound vitamin additive 1 part, medical stone powder 4 parts, composite mineral matter additive 0.5 part, phytase 0.05 part, 2 parts, chitosan powder, Chinese herbal feed additive 2 parts.
Further preferably, the bulk drug of described Chinese herbal feed additive comprise king taro, golden cypress, adenophora tetraphylla, saline cistanche, hawthorn, the coptis, ti, ajuga decumbens, Asiatic plantain, clarke boea herb, prolong grass, franzenbach rhubarb root and rhizome, Radix Angelicae Sinensis, white slurry rattan and Radix Glycyrrhizae;
In described Chinese herbal feed additive the weight portion of each bulk drug be respectively king taro 25 ~ 35 parts, golden cypress 20 ~ 30 parts, adenophora tetraphylla 15 ~ 25 parts, saline cistanche 15 ~ 25 parts, hawthorn 10 ~ 20 parts, the coptis 10 ~ 20 parts, ti 5 ~ 15 parts, ajuga decumbens 5 ~ 15 parts, Asiatic plantain 5 ~ 15 parts, clarke boea herb 10 ~ 20 parts, prolong grass 15 ~ 25 parts, franzenbach rhubarb root and rhizome 5 ~ 15 parts, Radix Angelicae Sinensis 15 ~ 25 parts, white slurry 10 ~ 20 parts, rattan and 5 ~ 15 parts, Radix Glycyrrhizae.
Further preferably, described Chinese herbal feed additive is only prepared from by above-mentioned raw materials medicine.
Further preferably, in described Chinese herbal feed additive the weight portion of each bulk drug be respectively king taro 28 ~ 32 parts, golden cypress 23 ~ 27 parts, adenophora tetraphylla 18 ~ 22 parts, saline cistanche 18 ~ 22 parts, hawthorn 13 ~ 17 parts, the coptis 13 ~ 17 parts, ti 8 ~ 12 parts, ajuga decumbens 8 ~ 12 parts, Asiatic plantain 8 ~ 12 parts, clarke boea herb 13 ~ 17 parts, prolong grass 18 ~ 22 parts, franzenbach rhubarb root and rhizome 8 ~ 12 parts, Radix Angelicae Sinensis 18 ~ 22 parts, white slurry 13 ~ 17 parts, rattan and 8 ~ 12 parts, Radix Glycyrrhizae.
Most preferably, in described Chinese herbal feed additive the weight portion of each bulk drug be respectively king taro 30 parts, golden cypress 25 parts, adenophora tetraphylla 20 parts, saline cistanche 20 parts, hawthorn 15 parts, the coptis 15 parts, ti 10 parts, ajuga decumbens 10 parts, Asiatic plantain 10 parts, clarke boea herb 15 parts, prolong grass 20 parts, franzenbach rhubarb root and rhizome 10 parts, Radix Angelicae Sinensis 20 parts, white slurry 15 parts, rattan and 10 parts, Radix Glycyrrhizae.
Present invention also offers the preparation method of said herbal medicine feed addictive, be specially:
Take each bulk drug according to described weight portion in grams, pulverize, mix, obtain mixture, decoct every 500 grams of mixtures first and add cold water 800-1000 milliliter, by whole raw material soaking after 30 points to 60 minutes, 30 minutes at a simmer, leach concoction; Add cold water 500-800 milliliter slow fire again and decoct 20-25 minute again, leach concoction for twice and mix even, concentrated removal moisture, dry, pulverize subsequently, obtain Chinese herbal feed additive.
Present invention also offers the preparation method of lake, the female mountain steamed crab breeding feed of above-mentioned raising meat nutrition, it comprises:
The first step, takes black carp powder, medical stone powder, chitosan powder, cornstarch in proportion, mixes rear pulverizing, crosses 45-55 mesh sieve, obtains crushed material;
Second step, take Choline Chloride, salt, calcium monohydrogen phosphate, water earthworm powder, compound vitamin additive, composite mineral matter additive, phytase, Chinese herbal feed additive in proportion, crushed material obtained by itself and the first step is mixed to evenly, obtains raw mixture;
3rd step, carries out modifier treatment by the raw mixture obtained by second step, and refining temperature is 60-68 DEG C, and the modified humidity to raw mixture is 13-15%;
4th step, sends the raw mixture after the 3rd step matter into feed-processing equipment and is processed into granular mixed feed;
5th step, the granular mixed feed of the 4th step gained is dry under 80 DEG C of conditions, be packaged into bag after being then cooled to room temperature.
Described compound vitamin additive can adopt commercially available any aquatic products compound vitamin additive, what adopt in the present invention is the aquatic products multidimensional that Zheng Zhoudong herds chemical products Co., Ltd and provides, and its main component is (every kilogram of content): retinol1 100000IU; Vitamine D3 320000IU; Nicotinic acid 7800mg; Vitamin E2 500mg; VB11 000mg; Biotin 8mg; Vitamin B2 2000mg; Vitamin B6 1000mg; Folic acid 400mg; Vitamin B12 125mg; Vitamin C 18000mg; Enzyme 1000mg; Potassium 1.1%; Salt 4.5% methionine 400mg; Moisture≤10%.
Described composite mineral matter additive can adopt commercially available any aquatic products composite mineral matter additive, the nutrition Shu Le that Bao Ji bio tech ltd, preferred Hangzhou provides, raw material composition comprises calcium, iron, zinc, selenium, iodine, manganese, bacillus subtilis, lactobacillus acidophilus, montmorillonite.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.
Embodiment 1 Chinese herbal feed additive
Take king taro 30g, golden cypress 25g, adenophora tetraphylla 20g, saline cistanche 20g, hawthorn 15g, coptis 15g, ti 10g, ajuga decumbens 10g, Asiatic plantain 10g, clarke boea herb 15g, prolong careless 20g, franzenbach rhubarb root and rhizome 10g, Radix Angelicae Sinensis 20g, white slurry rattan 15g and Radix Glycyrrhizae 10g, pulverize, mix, obtain mixture, decoct every 500g mixture first and add 1000 milliliters, cold water, by whole raw material soaking after 60 minutes, 30 minutes at a simmer, leach concoction; Add cold water 800 milliliters of slow fire again and decoct 25 minutes again, leach concoction for twice and mix even, concentrated removal moisture, dry, pulverize subsequently, obtain Chinese herbal feed additive.
Toxicity detection
1, acute toxicity test
Application mouse carries out acute toxicity testing and shows: Mouse oral gavage Chinese herbal feed additive of the present invention, under 488.8g crude drug/kg dosage, after administration, mouse occurs that light activity reduces, 1 hours recovers normal, Continuous Observation 7 days after administration, none animal dead, its overall health of patients, diet, take the photograph water, urine and body weight increase all normal, experimental result shows: the maximum dosage-feeding of Mouse oral gavage Chinese herbal feed additive of the present invention is 488.8g crude drug/kg/d (LD
50> 488.8g crude drug/kg).Point out this feed addictive acute toxicity low, clinical drug safety.
2, long term toxicity test
Select SD rat, give the Chinese herbal feed additive of the present invention of variable concentrations (18.0,6.0,2.0g crude drug/kg), every day gavage once, continuous 90 days, within after last administration 24 hours, respectively group is alive kills 1/2 animal (male and female half and half), and all the other 1/2 animals continue observations 2 weeks rear living and kill.Duration of test observes outward appearance, general behavior, food ration, the changes of weight of animal, within after administration 90 days, within 2 weeks, carries out the index checking such as hematology (RBC, HB, granulophilocyte, PLT, CT, WBC and classification) and blood biochemical (AST, ALT, ALP, Glu, BUN, Crea, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficient, Histopathology with drug withdrawal.Result of the test shows: preparation of the present invention is good at high, medium and low dosage treated animal general state, and outward appearance sign, behavioral activity, food-intake and body weight increase change all without exception; Three dosage groups and control group hematological examination, blood biochemical analysis, urine biochemical analysis all in normal range (NR), without significant difference between group; Each group of main organs histopathological examination shows no obvious abnormalities.These parameters drug withdrawal also had no change after 2 weeks.Rat is had no significant effect in high, medium and low three dosage (18.0,6.0,2.0g crude drug/kg) administration in continuous 90 days according to result of the test Chinese medicine preparation of the present invention, without clear and definite toxicity target organ and sensitive indicator, convalescence observation also has no retardance toxic reaction, points out Chinese herbal feed additive security of the present invention higher.
Embodiment 2 feed 1
Take black carp powder 350g, medical stone powder 40g, chitosan powder 20g, cornstarch 100g, mix rear pulverizing, cross 55 mesh sieves, obtain crushed material, take Choline Chloride 10g, salt 40g, calcium monohydrogen phosphate 20g, water earthworm powder 80g, compound vitamin additive aquatic products multidimensional 10g, composite mineral matter additive nutrition Shu Le 5g, phytase 0.5g, Chinese herbal feed additive 20g prepared by embodiment 1 is added in crushed material above, obtain raw mixture, obtained raw mixture is carried out modifier treatment, refining temperature is 60 DEG C, the modified humidity to raw mixture is 13%, raw mixture feeding feed-processing equipment after modified is processed into granular mixed feed, the granular mixed feed of gained is dry under 80 DEG C of conditions, then bag is packaged into after being cooled to room temperature.
Embodiment 3 feed 2
Take black carp powder 350g, medical stone powder 40g, chitosan powder 20g, cornstarch 100g, mix rear pulverizing, cross 55 mesh sieves, obtain crushed material, take Choline Chloride 10g, salt 40g, calcium monohydrogen phosphate 20g, water earthworm powder 20g, compound vitamin additive aquatic products multidimensional 10g, composite mineral matter additive nutrition Shu Le 5g, phytase 0.5g is added in crushed material above, obtain raw mixture, obtained raw mixture is carried out modifier treatment, refining temperature is 60 DEG C, the modified humidity to raw mixture is 13%, raw mixture feeding feed-processing equipment after modified is processed into granular mixed feed, the granular mixed feed of gained is dry under 80 DEG C of conditions, then bag is packaged into after being cooled to room temperature.
Embodiment 4 feeds data
Test site is certain characteristic aquaculture base.With plastics purse seine, a pond being separated into 6 sizes is 500m
2little pond, establish two processed group altogether, feed 1 prepared by embodiment of throwing something and feeding respectively 2 and feed 2 prepared by embodiment 3, often kind of bait arrange 3 parallel.The group # of feeding feed 1 is B, and the group # of feeding feed 2 is A.
Test is selected from steamed crab cultivation base, lake, female mountain with crab, and seed appendage perfects, and specification is (10.01
+1.02) g.Breeding density is 15000/hm
2, female-male proportion is 1:1.
Aquaculture management
Start normally to throw something and feed after water temperature rises to 15 DEG C, wherein two groups feed day feeding volume be 2% ~ 5% of crab gross weight, every day at dusk throws something and feeds once, adjusts feeding volume in time depending on weather conditions.Cultivate early stage controlling water level at about 1m, per week adding to two weeks, changes water once; High temperature time water level is increased to 1.3 ~ 1.5m, and adds weekly (changing) one to twice water, is about about 1/4 of 20 ~ 40cm or pond total Water at every turn.Periodic detection crab pond water quality condition, controls more than dissolved oxygen in water 3mg/L, pH=7.0 ~ 8.5, pond, ammonia nitrogen (NH
4 +-N) <0.2mg/L, nitrite (NO
2 --N) <0.1mg/L, hydrogen sulfide (S
2-) <0.001mg/L.
Sample collection and analysis
Culture experiment started from May 20th, 2013, to end of day November 5 in 2013, lasted 231d altogether, taked each 20 of male and female crab at random, Weighing body quality at the end of cultivation from each test tank, and measurement shell is long, shell is wide and record.Carry out vivisection subsequently, take the tissues such as hepatopancrease, sexual gland, muscle, calculate the indexs such as hepatopancreatic cell (HSI), genital gland indices (GSI), dressing percentage (%).And fresh hepatopancrease, sexual gland and muscle are deposited in-20 DEG C of refrigerators be used for biochemical measurement.
Hepatopancreatic cell (HIS, %)=hepatopancrease weight/crab weight × 100
Genital gland indices (GSI, %)=sexual gland weight/crab weight × 100
Dressing percentage (%)=muscle weight/crab weight × 100
105 DEG C, drying box is dried and is measured moisture, the each tissue protein content of Kjeldahl nitrogen determination, by Folch method (FolchJ, LeesM, SloaneStanleyG.Asimplemethodfortheisolationandpurificati onoftotallipidsfromanimaltissues.JournalofBiologicalChem istry, 1957, 226 (1): 497-509) fat content is measured, lipid and fatty acid analysis (Wu Xugan is carried out by methods such as Wu Xugan, Cheng Yongxu, Tang Baiping, Deng Onchidium struma lay eggs front and back lipid and aliphatic acid composition change, animal journal, 2007, 53 (6): 1089-1100), lipid component and content of fatty acid calculate and adopt area percentage method.
Use SPSS15.0 statistical software carry out analyzing and processing, measurement data with
(average ± standard deviation) represents, meets normal distribution person and adopts t to check, do not meet normal distribution person and adopt rank test, and enumeration data adopts
x 2inspection, with P < 0.05 for difference has statistical significance.
Growth indexes result
Two groups of crab situations of growing compare, and the results are shown in Table 1.
The situation of growing of table 1 liang group crab compares
As can be seen from Table 1, adopt feed 1 provided by the invention to carry out feeding, the survival rate of crab, output, dressing percentage and weight are not more added Chinese herbal feed additive and are significantly improved, and nutritive value is higher, p<0.05.
Two groups of crab Body components compare, and the results are shown in Table 2.
The table 2 liang each organizational routine biochemical composition of group crab body compares
As can be seen from Table 2, adopt the feed containing Chinese herbal feed additive provided by the invention can significantly improve crude fat content in hepatopancrease, sexual gland and muscle and crude protein content, with the group significant difference not adding Chinese herbal feed additive, be more conducive to absorption and the deposition of lake, female mountain steamed crab body fat.
Two groups of crab lipid ratio of components comparatively, the results are shown in Table 3.
Table 3 liang group crab edible part lipid composition
Lipid is the important energy reserves material of crustacean, and wherein triglycerides is main storage form.Table 3 shows, and adopt the group hepatopancrease of Chinese herbal feed additive, sexual gland and muscle content of triglyceride all higher than the group of not adding Chinese herbal feed additive, show that the energy accumulation of B group is better than A group, nutritive value improves greatly.
Immune indexes is analyzed
The immune response of Shrimp waste crustacean depends on nospecific immunity, nospecific immunity enzyme has important function in body defenses system, and the immunoenzymatic oxidation resistance power such as superoxide dismutase, catalase and glutathione peroxidase reflects the immune state of animal body indirectly.
Activities of antioxidant enzymes is analyzed: fracture from young crab foot of a chela base joint and get hemolymph, be placed in Eppendorf pipe and spend the night at 4 DEG C, and after high speed freezing centrifuge is centrifugal, to put into-80 DEG C of preservations to be measured for sucking-off serum.The kit that the active mensuration of TAC T-AOC in serum and hepatopancrease, musculature, superoxide dismutase (SOD), MDA (MDA), catalase (CAT) all adopts Nanjing, Jiangsu Province to build up biological study to produce, assay method carries out with reference to description.
The impact of table 4 serum anti-oxidation function
Table 4 can be found out, adopt feed provided by the invention can significantly improve TAC T-AOC, superoxide dismutase (SOD), MDA (MDA), catalase (CAT) level in crab serum, comparatively A group significant difference, p<0.05.
Table 5 muscle anti-oxidation function affects
Table 5 can be found out, adopt feed provided by the invention can significantly improve TAC T-AOC, superoxide dismutase (SOD), MDA (MDA), catalase (CAT) level in crab muscle, comparatively A group significant difference, p<0.05.
Table 6 hepatopancrease anti-oxidation function affects
Table 6 can be found out, adopt feed provided by the invention can significantly improve TAC T-AOC, superoxide dismutase (SOD), MDA (MDA), catalase (CAT) level in crab hepatopancrease, comparatively A group significant difference, p<0.05.
All above-mentioned this intellectual properties of primary enforcement, not setting restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (6)
1. one kind is improved lake, the female mountain steamed crab breeding feed of meat nutrition, it is characterized in that, comprising: Choline Chloride, salt, calcium monohydrogen phosphate, black carp powder, cornstarch, water earthworm powder, compound vitamin additive, medical stone powder, composite mineral matter additive, phytase, chitosan powder and Chinese herbal feed additive;
The weight portion of each raw material is respectively Choline Chloride 0.5 ~ 1.5 part, salt 3 ~ 5 parts, calcium monohydrogen phosphate 1 ~ 3 part, 30 ~ 40 parts, black carp powder, cornstarch 5 ~ 15 parts, water earthworm powder 5 ~ 10 parts, compound vitamin additive 0.5 ~ 1.5 part, medical stone powder 3 ~ 5 parts, composite mineral matter additive 0.3 ~ 0.6 part, phytase 0.03 ~ 0.1 part, 1 ~ 3 part, chitosan powder, Chinese herbal feed additive 1 ~ 3 part.
2. lake, the female mountain steamed crab breeding feed improving meat nutrition as claimed in claim 1, is characterized in that: in lake, the female mountain steamed crab breeding feed of described raising meat nutrition, the weight portion of each raw material is respectively Choline Chloride 1 part, salt 4 parts, calcium monohydrogen phosphate 2 parts, 35 parts, black carp powder, cornstarch 10 parts, water earthworm powder 8 parts, compound vitamin additive 1 part, medical stone powder 4 parts, composite mineral matter additive 0.5 part, phytase 0.05 part, 2 parts, chitosan powder, Chinese herbal feed additive 2 parts.
3. as claimed in claim 1 lake, the female mountain steamed crab breeding feed improving meat nutrition, is characterized in that: the bulk drug of described Chinese herbal feed additive comprises king taro, golden cypress, adenophora tetraphylla, saline cistanche, hawthorn, the coptis, ti, ajuga decumbens, Asiatic plantain, clarke boea herb, prolongs grass, franzenbach rhubarb root and rhizome, Radix Angelicae Sinensis, white slurry rattan and Radix Glycyrrhizae;
In described Chinese herbal feed additive the weight portion of each bulk drug be respectively king taro 25 ~ 35 parts, golden cypress 20 ~ 30 parts, adenophora tetraphylla 15 ~ 25 parts, saline cistanche 15 ~ 25 parts, hawthorn 10 ~ 20 parts, the coptis 10 ~ 20 parts, ti 5 ~ 15 parts, ajuga decumbens 5 ~ 15 parts, Asiatic plantain 5 ~ 15 parts, clarke boea herb 10 ~ 20 parts, prolong grass 15 ~ 25 parts, franzenbach rhubarb root and rhizome 5 ~ 15 parts, Radix Angelicae Sinensis 15 ~ 25 parts, white slurry 10 ~ 20 parts, rattan and 5 ~ 15 parts, Radix Glycyrrhizae.
4. as claimed in claim 3 lake, the female mountain steamed crab breeding feed improving meat nutrition, is characterized in that: in described Chinese herbal feed additive the weight portion of each bulk drug be respectively king taro 28 ~ 32 parts, golden cypress 23 ~ 27 parts, adenophora tetraphylla 18 ~ 22 parts, saline cistanche 18 ~ 22 parts, hawthorn 13 ~ 17 parts, the coptis 13 ~ 17 parts, ti 8 ~ 12 parts, ajuga decumbens 8 ~ 12 parts, Asiatic plantain 8 ~ 12 parts, clarke boea herb 13 ~ 17 parts, prolong grass 18 ~ 22 parts, franzenbach rhubarb root and rhizome 8 ~ 12 parts, Radix Angelicae Sinensis 18 ~ 22 parts, white slurry 13 ~ 17 parts, rattan and 8 ~ 12 parts, Radix Glycyrrhizae.
5. as claimed in claim 4 lake, the female mountain steamed crab breeding feed improving meat nutrition, is characterized in that: in described Chinese herbal feed additive the weight portion of each bulk drug be respectively king taro 30 parts, golden cypress 25 parts, adenophora tetraphylla 20 parts, saline cistanche 20 parts, hawthorn 15 parts, the coptis 15 parts, ti 10 parts, ajuga decumbens 10 parts, Asiatic plantain 10 parts, clarke boea herb 15 parts, prolong grass 20 parts, franzenbach rhubarb root and rhizome 10 parts, Radix Angelicae Sinensis 20 parts, white slurry 15 parts, rattan and 10 parts, Radix Glycyrrhizae.
6. the preparation method of lake, the female mountain steamed crab breeding feed of claim 1 to 5 arbitrary described raising meat nutrition, is characterized in that, comprising:
The first step, takes black carp powder, medical stone powder, chitosan powder, cornstarch in proportion, mixes rear pulverizing, crosses 45-55 mesh sieve, obtains crushed material;
Second step, take Choline Chloride, salt, calcium monohydrogen phosphate, water earthworm powder, compound vitamin additive, composite mineral matter additive, phytase, Chinese herbal feed additive in proportion, crushed material obtained by itself and the first step is mixed to evenly, obtains raw mixture;
3rd step, carries out modifier treatment by the raw mixture obtained by second step, and refining temperature is 60-68 DEG C, and the modified humidity to raw mixture is 13-15%;
4th step, sends the raw mixture after the 3rd step matter into feed-processing equipment and is processed into granular mixed feed;
5th step, the granular mixed feed of the 4th step gained is dry under 80 DEG C of conditions, be packaged into bag after being then cooled to room temperature.
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Application publication date: 20151202 |