CN105767600A - Immunity-enhancing rice-field eel feed and preparation method thereof - Google Patents
Immunity-enhancing rice-field eel feed and preparation method thereof Download PDFInfo
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Abstract
The invention provides an immunity-enhancing rice-field eel feed. The immunity-enhancing rice-field eel feed comprises the following ingredients: choline chloride, table salt, calcium hydrogen phosphate, gluten powder, corn starch, tubificidae powder, a compound vitamin additive, roe powder, a compound mineral additive, Pu-erh tea residues, esterified vitamin C and a Chinese herbal medicine feed additive. The Chinese herbal medicines can be utilized in the feed so as to improve immunities of the rice-field eels; the compound vitamin additive and the compound mineral additive are matched in the feed so as to regulate nutrients in the rice-field eel bodies, so that meat textures and tastes of the rice-field eels are improved; moreover, the Pu-erh tea residues, the tubificidae powder, the roe powder and the esterified vitamin C are matched, so that the feed can be absorbed by the rice-field eels more easily after eating without causing anorexic conditions.
Description
Technical field
The present invention relates to aquatic feeds technical field, particularly relate to a kind of ricefield el fodder strengthening immunity and preparation method thereof.
Background technology
Monopteri albi (formal name used at school: Monopterusalbus) belongs to closes gill fish eyes, closes gill Gyrinocheilidae, Monopterus.Also known as Monopterus albus (Zuiew), fish, sieve Chinese yellow eel, Serpentis fish, blood Chinese yellow eel, normal fish, [1] Changyu (northern Suzhou one is with).Close the general designation of the elongated eel shape Fish of gill fish eyes about 15 kinds.China is distributed two kinds, a kind of is common Monopterus albus (Zuiew), and also having one is mountain Monopterus albus (Zuiew), in river, cloud, expensive, Chongqing, Hunan, Hubei Province, Anhui, the ground such as Henan and Guangdong and Guangxi Provinces have distribution.Monopteri albi is exactly the Monopterus albus (Zuiew) being commonly called as, and belongs to Osteichthyes, Symbranchir, He Sai section, Monopterus albus (Zuiew) subfamily.Being a kind of fish, health is as Serpentis, but does not have squama, and the colour of skin has green grass or young crops, Huang two kinds, and big has two, three chis long.Like quiet, generally repose at the bottom.
At present, aquaculture is one of whole world agricultural with fastest developing speed, thus the development of high-efficiency feed is played a significant role.In addition, along with developing rapidly of culture of swamp eel industry, the traditional bait such as trash fish declines due to stock number, nutritional labeling is unbalanced, the reasons such as health quality can not be guaranteed cannot meet its huge cultivation demand, and the development of novel fodder and popularization and application have become as the key factor promoting culture of swamp eel industry sustainable health development.
For reaching to strengthen Monopterus albus (Zuiew) immune function, improve the purpose of Monopterus albus (Zuiew) premunition, the immunologic mechanism of research Monopterus albus (Zuiew), carry out the research and development of immunostimulant, be carried out one of effective means of organic aquaculture standard.The disease-prevention health effect of Chinese medicine is it will be evident that reason is containing the composition strengthening immunologic function in Chinese medicine material, can effectively regulate non-specific antimicrobial antiviral agent in animal body.It is therefore possible to use Chinese herbal medicine companion's bait is thrown something and fed and started and regulate the nonspecific immunity of Monopterus albus (Zuiew), improve himself immunocompetence.Meanwhile, the interpolation of Chinese herbal medicine can also adjust the nutritional labeling in Finless Eel Monopterus Albus, improves meat and mouthfeel.But light has Chinese herbal medicine additive, affecting again the alimentation of Monopterus albus (Zuiew), Monopterus albus (Zuiew) produces the situation of anorexia, causes that Monopterus albus (Zuiew) increases unhappy.
Summary of the invention
The technical problem to be solved is to provide a kind of ricefield el fodder strengthening immunity, it contains Chinese herbal medicine additive composition, Chinese herbal medicine can be utilized effectively to strengthen the immunocompetence of Monopterus albus (Zuiew), the nutritional labeling in Finless Eel Monopterus Albus can be adjusted simultaneously, improve meat and mouthfeel, then add other compatibilizing ingredient, make the effect of Chinese herbal medicine additive perform to maximum.
For solving above-mentioned technical problem, the invention provides a kind of ricefield el fodder strengthening immunity, including: choline chloride, Sal, calcium hydrogen phosphate, gluten meal, corn starch, Limnodrilus hoffmeisteri powder, compound vitamin additive, roe powder, composite mineral matter additive, puer tea slag, esterification vitamin C and Chinese herbal feed additive；
The weight portion of each raw material respectively choline chloride 0.5～1.5 part, Sal 3～5 parts, calcium hydrogen phosphate 1～3 part, gluten meal 30～40 parts, corn starch 5～15 parts, 1～3 part of Limnodrilus hoffmeisteri powder, compound vitamin additive 0.5～1.5 part, 20～30 parts of roe powder, composite mineral matter additive 0.3～0.6 part, puer tea slag 0.03～0.1 part, esterification vitamin C 10～20 parts, Chinese herbal feed additive 2～4 parts.
Wherein, the weight portion respectively choline chloride 1 part of each raw material, Sal 4 parts, calcium hydrogen phosphate 2 parts, gluten meal 35 parts, corn starch 10 parts, 2 parts of Limnodrilus hoffmeisteri powder, compound vitamin additive 1 part, 25 parts of roe powder, composite mineral matter additive 0.5 part, puer tea slag 0.05 part, esterification vitamin C 15 parts, Chinese herbal feed additive 3 parts in the described ricefield el fodder strengthening immunity.
Wherein, the crude drug of described Chinese herbal feed additive include Hippocampus, Rhizoma Dioscoreae, Radix Adenophorae, cloud roller, Fructus Crataegi, white Rhizoma Curculiginis, dried silkworm chrysalis meal, Rhizoma Arisaematis, DIGEN, Radix Rehmanniae Preparata, prolong grass, Radix et Rhizoma Rhei Franzenbachii, Radix Angelicae Sinensis, Fructus Trichosanthis, white slurry rattan and Radix Buddlejae Butterflybush；
The weight portion of each crude drug respectively Hippocampus 25～35 parts, Rhizoma Dioscoreae 20～30 parts, Radix Adenophorae 15～25 parts, cloud roller 15～25 parts, Fructus Crataegi 10～20 parts, white Rhizoma Curculiginis 10～20 parts, dried silkworm chrysalis meal 5～15 parts, Rhizoma Arisaematis 5～15 parts, DIGEN 5～15 parts, Radix Rehmanniae Preparata 10～20 parts in described Chinese herbal feed additive, prolong grass 15～25 parts, Radix et Rhizoma Rhei Franzenbachii 5～15 parts, Radix Angelicae Sinensis 15～25 parts, Fructus Trichosanthis 10～20 parts, white 10～20 parts of rattan of slurry and Radix Buddlejae Butterflybush 5～15 parts.
Wherein, the weight portion of each crude drug respectively Hippocampus 28～32 parts, Rhizoma Dioscoreae 23～27 parts, Radix Adenophorae 18～22 parts, cloud roller 18～22 parts, Fructus Crataegi 13～17 parts, white Rhizoma Curculiginis 13～17 parts, dried silkworm chrysalis meal 8～12 parts, Rhizoma Arisaematis 8～12 parts, DIGEN 8～12 parts, Radix Rehmanniae Preparata 13～17 parts in described Chinese herbal feed additive, prolong grass 18～22 parts, Radix et Rhizoma Rhei Franzenbachii 8～12 parts, Radix Angelicae Sinensis 18～22 parts, Fructus Trichosanthis 13～17 parts, white 13～17 parts of rattan of slurry and Radix Buddlejae Butterflybush 8～12 parts.
Wherein, the weight portion of each crude drug respectively hippocampus 30 part, Rhizoma Dioscoreae 25 parts, Radix Adenophorae 20 parts, cloud roller 20 parts, Fructus Crataegi 15 parts, white Rhizoma Curculiginis 15 parts, dried silkworm chrysalis meal 10 parts, Rhizoma Arisaematis 10 parts, DIGEN 10 parts, Radix Rehmanniae Preparata 15 parts in described Chinese herbal feed additive, prolong grass 20 parts, Radix et Rhizoma Rhei Franzenbachii 10 parts, Radix Angelicae Sinensis 20 parts, Fructus Trichosanthis 15 parts, white 15 parts of rattan of slurry and Radix Buddlejae Butterflybush 10 parts.
The preparation method that present invention also offers said herbal medicine feed additive, particularly as follows:
Weighing each crude drug according to described weight portion in grams, pulverize, mix, it is thus achieved that mixture, decoct every 500 grams of mixture first and add cold water 800-1000 milliliter, after whole raw material soaking 30 points to 60 minutes, slow fire is decocted 30 minutes, leaches medicine juice；Adding cold water 500-800 milliliter slow fire to decoct 20-25 minute again, leach medicine juice for twice and mix even, moisture is removed in concentration, dry, pulverizes subsequently, it is thus achieved that Chinese herbal feed additive.
The preparation method that present invention also offers the ricefield el fodder of above-mentioned enhancing immunity, comprising:
The first step, weighs gluten meal, roe powder, esterification vitamin C, corn starch in proportion, pulverizes, cross 45-55 mesh sieve, obtain ground product after mix homogeneously；
Second step, weigh choline chloride, Sal, calcium hydrogen phosphate, Limnodrilus hoffmeisteri powder, compound vitamin additive, composite mineral matter additive, puer tea slag, Chinese herbal feed additive in proportion, it is mixed with the ground product obtained by the first step to uniformly, obtain raw mixture；
3rd step, carries out modifier treatment by the raw mixture obtained by second step, and refining temperature is 70-75 DEG C, and the modified humidity to raw mixture is 18-22%；
4th step, sends the raw mixture after the 3rd step matter into feed-processing equipment and is processed into granular mixed feed；
5th step, dries the granular mixed feed of the 4th step gained under 85 DEG C of conditions, is packaged into bag after being subsequently cooled to room temperature.
Beneficial effects of the present invention:
The immunocompetence that the ricefield el fodder strengthening immunity containing Chinese herbal feed additive provided by the invention can utilize Chinese herbal medicine effectively to strengthen Monopterus albus (Zuiew), coordinate compound vitamin additive, composite mineral matter additive and puer tea slag can adjust the nutritional labeling in Finless Eel Monopterus Albus simultaneously, improve meat and mouthfeel, and puer tea slag, Limnodrilus hoffmeisteri powder, roe powder and esterification vitamin C with the use of, Monopterus albus (Zuiew) is easier to after eating up absorb, and will not produce the situation of anorexia.
Detailed description of the invention
The invention provides a kind of ricefield el fodder strengthening immunity, including: choline chloride, Sal, calcium hydrogen phosphate, gluten meal, corn starch, Limnodrilus hoffmeisteri powder, compound vitamin additive, roe powder, composite mineral matter additive, puer tea slag, esterification vitamin C and Chinese herbal feed additive；
The weight portion of each raw material respectively choline chloride 0.5～1.5 part, Sal 3～5 parts, calcium hydrogen phosphate 1～3 part, gluten meal 30～40 parts, corn starch 5～15 parts, 1～3 part of Limnodrilus hoffmeisteri powder, compound vitamin additive 0.5～1.5 part, 20～30 parts of roe powder, composite mineral matter additive 0.3～0.6 part, puer tea slag 0.03～0.1 part, esterification vitamin C 10～20 parts, Chinese herbal feed additive 2～4 parts.
It is preferred that, the described ricefield el fodder strengthening immunity is only prepared from by above-mentioned raw materials.
It is preferred that, the weight portion respectively choline chloride 1 part of each raw material, Sal 4 parts, calcium hydrogen phosphate 2 parts, gluten meal 35 parts, corn starch 10 parts, 2 parts of Limnodrilus hoffmeisteri powder, compound vitamin additive 1 part, 25 parts of roe powder, composite mineral matter additive 0.5 part, puer tea slag 0.05 part, esterification vitamin C 15 parts, Chinese herbal feed additive 3 parts in the described ricefield el fodder strengthening immunity.
It is preferred that, the crude drug of described Chinese herbal feed additive includes Hippocampus, Rhizoma Dioscoreae, Radix Adenophorae, cloud roller, Fructus Crataegi, white Rhizoma Curculiginis, dried silkworm chrysalis meal, Rhizoma Arisaematis, DIGEN, Radix Rehmanniae Preparata, prolongs grass, Radix et Rhizoma Rhei Franzenbachii, Radix Angelicae Sinensis, Fructus Trichosanthis, white slurry rattan and Radix Buddlejae Butterflybush；
The weight portion of each crude drug respectively Hippocampus 25～35 parts, Rhizoma Dioscoreae 20～30 parts, Radix Adenophorae 15～25 parts, cloud roller 15～25 parts, Fructus Crataegi 10～20 parts, white Rhizoma Curculiginis 10～20 parts, dried silkworm chrysalis meal 5～15 parts, Rhizoma Arisaematis 5～15 parts, DIGEN 5～15 parts, Radix Rehmanniae Preparata 10～20 parts in described Chinese herbal feed additive, prolong grass 15～25 parts, Radix et Rhizoma Rhei Franzenbachii 5～15 parts, Radix Angelicae Sinensis 15～25 parts, Fructus Trichosanthis 10～20 parts, white 10～20 parts of rattan of slurry and Radix Buddlejae Butterflybush 5～15 parts.
It is preferred that, described Chinese herbal feed additive is only prepared from by above-mentioned raw materials medicine.
The weight portion of each crude drug respectively Hippocampus 28～32 parts, Rhizoma Dioscoreae 23～27 parts, Radix Adenophorae 18～22 parts, cloud roller 18～22 parts, Fructus Crataegi 13～17 parts, white Rhizoma Curculiginis 13～17 parts, dried silkworm chrysalis meal 8～12 parts, Rhizoma Arisaematis 8～12 parts, DIGEN 8～12 parts, Radix Rehmanniae Preparata 13～17 parts in described Chinese herbal feed additive, prolong grass 18～22 parts, Radix et Rhizoma Rhei Franzenbachii 8～12 parts, Radix Angelicae Sinensis 18～22 parts, Fructus Trichosanthis 13～17 parts, white 13～17 parts of rattan of slurry and Radix Buddlejae Butterflybush 8～12 parts.
Most preferably, the weight portion of each crude drug respectively hippocampus 30 part, Rhizoma Dioscoreae 25 parts, Radix Adenophorae 20 parts, cloud roller 20 parts, Fructus Crataegi 15 parts, white Rhizoma Curculiginis 15 parts, dried silkworm chrysalis meal 10 parts, Rhizoma Arisaematis 10 parts, DIGEN 10 parts, Radix Rehmanniae Preparata 15 parts in described Chinese herbal feed additive, prolong grass 20 parts, Radix et Rhizoma Rhei Franzenbachii 10 parts, Radix Angelicae Sinensis 20 parts, Fructus Trichosanthis 15 parts, white 15 parts of rattan of slurry and Radix Buddlejae Butterflybush 10 parts.
The preparation method that present invention also offers said herbal medicine feed additive, particularly as follows:
Weighing each crude drug according to described weight portion in grams, pulverize, mix, it is thus achieved that mixture, decoct every 500 grams of mixture first and add cold water 800-1000 milliliter, after whole raw material soaking 30 points to 60 minutes, slow fire is decocted 30 minutes, leaches medicine juice；Adding cold water 500-800 milliliter slow fire to decoct 20-25 minute again, leach medicine juice for twice and mix even, moisture is removed in concentration, dry, pulverizes subsequently, it is thus achieved that Chinese herbal feed additive.
The preparation method that present invention also offers the ricefield el fodder of above-mentioned enhancing immunity, comprising:
The first step, weighs gluten meal, roe powder, esterification vitamin C, corn starch in proportion, pulverizes, cross 45-55 mesh sieve, obtain ground product after mix homogeneously；
Second step, weigh choline chloride, Sal, calcium hydrogen phosphate, Limnodrilus hoffmeisteri powder, compound vitamin additive, composite mineral matter additive, puer tea slag, Chinese herbal feed additive in proportion, it is mixed with the ground product obtained by the first step to uniformly, obtain raw mixture；
3rd step, carries out modifier treatment by the raw mixture obtained by second step, and refining temperature is 70-75 DEG C, and the modified humidity to raw mixture is 18-22%；
4th step, sends the raw mixture after the 3rd step matter into feed-processing equipment and is processed into granular mixed feed；
5th step, dries the granular mixed feed of the 4th step gained under 85 DEG C of conditions, is packaged into bag after being subsequently cooled to room temperature.
Described compound vitamin additive can adopt commercially available any Aquatic product compound vitamin additive, what adopt in the present invention is the Zheng Zhoudong Aquatic product multidimensional herding that chemical products company limited provides, and it is mainly composed of (every kilogram of content): retinol1 100000IU；Vitamin D3 320000IU；Nicotinic acid 7800mg；Vitamin E2 500mg；VB11 000mg；Biotin 8mg；Vitamin B2 2000mg；Vitamin B6 1000mg；Folic acid 400mg；Vitamin B12 125mg；Vitamin C 18000mg；Enzyme 1000mg；Potassium 1.1%；Sal 4.5% methionine 400mg；Moisture≤10%.
Described composite mineral matter additive can adopt commercially available any Aquatic product composite mineral matter additive, the nutrition Shu Le that Bao Ji bio tech ltd, preferred Hangzhou provides, raw material composition includes calcium, ferrum, zinc, selenium, iodine, manganese, bacillus subtilis, bacillus acidophilus, montmorillonite.
Hereinafter adopting embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the process that realizes reaching technique effect can fully understand and implement according to this.
Embodiment 1 Chinese herbal feed additive
Weigh hippocampus 3 0g, Rhizoma Dioscoreae 25g, Radix Adenophorae 20g, cloud roller 20g, Fructus Crataegi 15g, white Rhizoma Curculiginis 15g, dried silkworm chrysalis meal 10g, Rhizoma Arisaematis 10g, DIGEN 10g, Radix Rehmanniae Preparata 15g, prolong grass 20g, Radix et Rhizoma Rhei Franzenbachii 10g, Radix Angelicae Sinensis 20g, Fructus Trichosanthis 15g, white slurry rattan 15g and Radix Buddlejae Butterflybush 10g, pulverize, mix, obtain mixture, decoct every 500g mixture first and add 1000 milliliters of cold water, after whole raw material soaking 60 minutes, slow fire is decocted 30 minutes, leaches medicine juice；Adding 800 milliliters of slow fire of cold water to decoct 25 minutes again, leach medicine juice for twice and mix even, moisture is removed in concentration, dry, pulverizes subsequently, it is thus achieved that Chinese herbal feed additive.
Toxicity detection
1, acute toxicity test
Application mice carries out acute toxicity testing and shows: the Chinese herbal feed additive of the Mouse oral gavage present invention, under 488.8g crude drug/kg dosage, after administration, mice occurs that light activity reduces, 1 hours recovers normal, Continuous Observation 7 days after administration, none animal dead, its overall health of patients, diet, take the photograph water, urine and body weight increase all normal, test result indicate that: the maximum dosage-feeding of the Chinese herbal feed additive of the Mouse oral gavage present invention is 488.8g crude drug/kg/d (LD50> 488.8g crude drug/kg).Point out this feed additive acute toxicity low, clinical drug safety.
2, long term toxicity test
Select SD rat, give the Chinese herbal feed additive of the present invention of variable concentrations (18.0,6.0,2.0g crude drug/kg), every day gavage once, continuous 90 days, last is administered latter 24 hours respectively to organize to live and kills 1/2 animal (male and female half and half), and all the other 1/2 animals continuation are lived after observing 2 weeks and killed.Observe the outward appearance of animal, general behavior, food ration, body weight change during test, be administered latter 90 days and drug withdrawal carries out the index checking such as hematology's (RBC, HB, reticulocyte, PLT, CT, WBC and classification) and blood biochemical (AST, ALT, ALP, Glu, BUN, Crea, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficient, histopathology for 2 weeks.Result of the test shows: the preparation of the present invention is good at high, medium and low dosage treated animal general state, and outward appearance sign, behavioral activity, food-intake and body weight increase change all without exception；Three dosage groups and matched group hematological examination, blood biochemical analysis, urine biochemical analysis all between normal range, group without significant difference；Each group main organs histopathological examination shows no obvious abnormalities.These parameters drug withdrawal also had no change after 2 weeks.Rat is had no significant effect by the Chinese medicine preparation according to the result of the test present invention in high, medium and low three dosage (18.0,6.0,2.0g crude drug/kg) administration in continuous 90 days, without clear and definite toxicity target organ and sensitive indicator, convalescent period observes and also has no retardance toxic reaction, and the Chinese herbal feed additive safety of the prompting present invention is higher.
Embodiment 2 feedstuff 1
nullWeigh gluten meal 350g、Roe powder 250g、Esterification vitamin C 150g、Corn starch 100g，Pulverize after mix homogeneously，Cross 55 mesh sieves，Obtain ground product，Weigh choline chloride 10g、Sal 40g、Calcium hydrogen phosphate 20g、Limnodrilus hoffmeisteri powder 20g、Compound vitamin additive Aquatic product multidimensional 10g、Composite mineral matter additive nutrition Shu Le 5g、Puer tea slag 0.5g、The Chinese herbal feed additive 30g of embodiment 1 preparation is added in ground product above，Obtain raw mixture，Obtained raw mixture is carried out modifier treatment，Refining temperature is 70 DEG C，The modified humidity to raw mixture is 18%，Raw mixture feeding feed-processing equipment after modified is processed into granular mixed feed，The granular mixed feed of gained is dried under 85 DEG C of conditions，It is packaged into bag after being subsequently cooled to room temperature.
Embodiment 3 feedstuff 2
Weigh gluten meal 350g, roe powder 250g, esterification vitamin C 150g, corn starch 100g, pulverize after mix homogeneously, cross 55 mesh sieves, obtain ground product, weigh choline chloride 10g, Sal 40g, calcium hydrogen phosphate 20g, Limnodrilus hoffmeisteri powder 20g, compound vitamin additive Aquatic product multidimensional 10g, composite mineral matter additive nutrition Shu Le 5g, puer tea slag 0.5g is added in ground product above, obtain raw mixture, obtained raw mixture is carried out modifier treatment, refining temperature is 70 DEG C, the modified humidity to raw mixture is 18%, raw mixture feeding feed-processing equipment after modified is processed into granular mixed feed, the granular mixed feed of gained is dried under 85 DEG C of conditions, it is packaged into bag after being subsequently cooled to room temperature.
Embodiment 4 feeds data
Test site is certain characteristic aquaculture base.With plastics purse seine, one pond is separated into 6 and is sized to 500m2Little pond, set two process groups altogether, the feedstuff 1 of embodiment of throwing something and feeding respectively 2 preparation and the feedstuff 2 of embodiment 3 preparation, every kind of bait arrange 3 parallel.The group # of feeding feedstuff 1 is B, and the group # of feeding feedstuff 2 is A.
Test is with Monopterus albus (Zuiew) purchased from certain button culture of swamp eel base, and seed perfects, and specification is (10.01 ± 1.02) g.Breeding density is 20000/hm2, female-male proportion is 1:1.
Aquaculture management
Start normally to throw something and feed after water temperature rises to 15 DEG C, wherein two groups feedstuff day feeding volume be the 2%～5% of Monopterus albus (Zuiew) gross weight, every day at dusk throws something and feeds once, adjusts feeding volume in time depending on weather conditions.Cultivation early stage controlling water level is at about 1m, and per week adding to two weeks changes water once；High temperature time water level increases to 1.3～1.5m, and adds weekly (changing) one to twice water, is about about the 1/4 of 20～40cm or pond total Water every time.Periodic detection Monopterus albus (Zuiew) pond water quality condition, controls more than dissolved oxygen in water 3mg/L, pH=7.0～8.5, pond, ammonia nitrogen (NH4 +-N) < 0.2mg/L, nitrite (NO2 --N) < 0.1mg/L, hydrogen sulfide (S2-)<0.001mg/L。
Sample collecting and analysis
Culture experiment started from July 25th, 2014, to end of day January 10 in 2016, lasts 231d altogether, takes Monopterus albus (Zuiew) 20, Weighing body quality at random from each test tank, measure shell length, shell width record when cultivation terminates.Carry out vivisection subsequently, take the tissues such as hepatopancrease, gonad, muscle, calculate the indexs such as hepatopancreatic cell (HSI), genital gland indices (GSI), dressing percentage (%).And fresh hepatopancrease, gonad and muscle deposited in-20 DEG C of refrigerators be used for biochemical measurement.
Hepatopancreatic cell (HIS, %)=hepatopancrease weight/Finless Eel Monopterus Albus quality × 100
Genital gland indices (GSI, %)=gonad weight/Finless Eel Monopterus Albus quality × 100
Dressing percentage (%)=muscle weight/Finless Eel Monopterus Albus quality × 100
null105 DEG C of drying baker is dried and is measured moisture，The each tissue protein content of Kjeldahl nitrogen determination，By Folch method (FolchJ,LeesM，SloaneStanleyG.Asimplemethodfortheisolationandpurificationoftotallipidsfromanimaltissues.JournalofBiologicalChemistry，1957，226 (1): 497-509) fat content is measured，Lipid and fatty acid analysis (Wu Xugan is carried out by methods such as Wu Xugan，Cheng Yongxu，Tang Baiping，Deng Onchidium struma lay eggs front and back lipid and fatty acid composition change，Animal journal，2007,53(6):1089-1100)，Lipid component and content of fatty acid calculate and adopt area percentage method.
Use SPSS15.0 statistical software be analyzed process, measurement data with(average ± standard deviation) represents, person adopts t to check to meet normal distribution, and person adopts rank test not to meet normal distribution, and enumeration data adopts X2Inspection, statistically significant for difference with P < 0.05.
Growth indexes result
Two groups of Monopterus albus (Zuiew) growth promoter situations compare, and result is in Table 1.
The growth promoter situation of 1 liang of table group Monopterus albus (Zuiew) compares
| Index | A group | B group |
| Survival rate/% | 75.64±2.18 | 83.15±1.85 |
| Yield/(kg/hm2) | 7257.6±20.13 | 9473.4±24.80 |
| Weight/kg | 0.48±0.15 | 0.57±0.16 |
| Hepatopancreatic cell/% | 8.71±0.72 | 7.08±0.91 |
| Property pancreas index/% | 5.99±1.01 | 6.32±0.86 |
| Dressing percentage/% | 20.93±1.15 | 28.31±1.12 |
As it can be seen from table 1 adopt feedstuff 1 provided by the invention to carry out feeding, the survival rate of Monopterus albus (Zuiew), yield, dressing percentage and weight are less added Chinese herbal feed additive and are significantly improved, p < 0.05.
Two groups of Finless Eel Monopterus Albus comparison of ingredients, result is in Table 2.
2 liang of the table group each organizational routine biochemical composition of Finless Eel Monopterus Albus compares
| Index | A group | B group | |
| Hepatopancrease | Moisture/weight in wet base | 56.28±3.93 | 46.16±2.63 |
| Crude fat/dry weight | 60.20±2.85 | 73.92±3.06 | |
| Crude protein/dry weight | 21.83±1.06 | 28.39±2.03 | |
| Gonad | Moisture/weight in wet base | 46.95±1.09 | 43.68±1.73 |
| Crude fat/dry weight | 31.93±1.02 | 38.07±1.35 | |
| Crude protein/dry weight | 53.67±0.98 | 59.25±1.23 | |
| Muscle | Moisture/weight in wet base | 75.33±1.08 | 75.01±1.92 |
| Crude fat/dry weight | 4.99±0.15 | 6.19±0.53 | |
| Crude protein/dry weight | 73.25±1.38 | 78.02±1.25 |
As can be seen from Table 2, adopt the feedstuff containing Chinese herbal feed additive provided by the invention can significantly improve the crude fat content in hepatopancrease, gonad and muscle and crude protein content, with the group significant difference not adding Chinese herbal feed additive, be more beneficial for absorption and the deposition of Finless Eel Monopterus Albus fat.
Relatively, result is in Table 3 for two groups of Monopterus albus (Zuiew) lipid ratio of componentss.
3 liang of table group Monopterus albus (Zuiew) edible part lipid composition
| Index | A group | B group | |
| Hepatopancrease | Triglyceride | 95.93±2.05 | 96.13±1.79 |
| Free fatty | 0.29±0.06 | 0.51±0.09 | |
| Cholesterol | 0.55±0.11 | 0.69±0.17 | |
| Phospholipid | 3.69±0.58 | 2.17±0.49 | |
| Gonad | Triglyceride | 31.98±1.06 | 43.65±1.93 |
| Free fatty | 2.75±0.16 | 3.68±0.08 | |
| Cholesterol | 1.63±0.26 | 1.98±0.35 | |
| Phospholipid | 66.71±2.93 | 70.85±1.96 | |
| Muscle | Triglyceride | 1.73±0.39 | 3.15±0.51 |
| Free fatty | 1.17±0.19 | 1.36±0.21 | |
| Cholesterol | 2.85±0.37 | 3.97±0.51 | |
| Phospholipid | 95.03±2.77 | 93.79±2.63 |
Lipid is to be good at the energy reserves material that class animal is important, and wherein triglyceride is main storage form.Table 3 shows, adopts the group hepatopancrease of Chinese herbal feed additive, gonad and muscle content of triglyceride to be above not adding the group of Chinese herbal feed additive, it was shown that B group energy accumulation is better than A group.
Immune indexes is analyzed
The reaction of Monopterus albus (Zuiew) class animal immune depends on nonspecific immunity, nonspecific immunity enzyme has important function in body defenses system, and the immunoenzymatic oxidation resistance such as superoxide dismutase, catalase and glutathion peroxidase power reflects the immune state of animal body indirectly.
Activities of antioxidant enzymes is analyzed: extracts hemolymph from Monopterus albus (Zuiew), is placed in Eppendorf pipe at 4 DEG C overnight, and after High speed refrigerated centrifuge is centrifugal, to put into-85 DEG C of preservations to be measured for sucking-off serum.Total antioxidant capacity T-AOC in serum and hepatopancrease, muscular tissue, superoxide dismutase (SOD), malonaldehyde (MDA), catalase (CAT) activity mensuration all adopt Nanjing, Jiangsu Province to build up the test kit that biological study produces, assay method carries out with reference to description.
The impact of table 4 serum anti-oxidation function
Table 4 can be seen that, adopt feedstuff provided by the invention can significantly improve the total antioxidant capacity T-AOC in Monopterus Albus, superoxide dismutase (SOD), malonaldehyde (MDA), catalase (CAT) level, relatively A group significant difference, p < 0.05.
Table 5 muscle anti-oxidation function affects
| Group | T-AOC(U/mg) | SOD(U/mg) | MDA(mmol/mg) | CAT(U/mg) |
| A group | 0.14±0.01 | 91.36±3.40 | 0.14±0.02 | 6.02±0.98 |
| B group | 0.28±0.02 | 128.59±3.71 | 0.16±0.02 | 6.75±1.02 |
Table 5 can be seen that, adopt feedstuff provided by the invention can significantly improve the total antioxidant capacity T-AOC in Monopterus albus (Zuiew) muscle, superoxide dismutase (SOD), malonaldehyde (MDA), catalase (CAT) level, relatively A group significant difference, p < 0.05.
Table 6 hepatopancrease anti-oxidation function affects
| Group | T-AOC(U/mg) | SOD(U/mg) | MDA(mmol/mg) | CAT(U/mg) |
| A group | 0.38±0.02 | 112.73±6.82 | 0.95±0.15 | 28.13±3.92 |
| B group | 0.57±0.03 | 136.92±5.88 | 1.31±0.02 | 34.48±2.18 |
Table 6 can be seen that, adopt feedstuff provided by the invention can significantly improve the total antioxidant capacity T-AOC in Monopterus albus (Zuiew) hepatopancrease, superoxide dismutase (SOD), malonaldehyde (MDA), catalase (CAT) level, relatively A group significant difference, p < 0.05.
The above, be only presently preferred embodiments of the present invention, is not the restriction that the present invention makees other form, and any those skilled in the art are changed or be modified as the Equivalent embodiments of equivalent variations possibly also with the technology contents of the disclosure above.But every without departing from technical solution of the present invention content, according to any simple modification, equivalent variations and remodeling that above example is made by the technical spirit of the present invention, still fall within the protection domain of technical solution of the present invention.
Claims (5)
1. the ricefield el fodder strengthening immunity, it is characterized in that, raw material consists of: choline chloride, Sal, calcium hydrogen phosphate, gluten meal, corn starch, Limnodrilus hoffmeisteri powder, compound vitamin additive, roe powder, composite mineral matter additive, puer tea slag, esterification vitamin C and Chinese herbal feed additive；
The weight portion of each raw material respectively choline chloride 0.5～1.5 part, Sal 3～5 parts, calcium hydrogen phosphate 1～3 part, gluten meal 30～40 parts, corn starch 5～15 parts, 1～3 part of Limnodrilus hoffmeisteri powder, compound vitamin additive 0.5～1.5 part, 20～30 parts of roe powder, composite mineral matter additive 0.3～0.6 part, puer tea slag 0.03～0.1 part, esterification vitamin C 10～20 parts, Chinese herbal feed additive 2～4 parts；
The weight portion of each crude drug respectively Hippocampus 25～35 parts, Rhizoma Dioscoreae 20～30 parts, Radix Adenophorae 15～25 parts, cloud roller 15～25 parts, Fructus Crataegi 10～20 parts, white Rhizoma Curculiginis 10～20 parts, dried silkworm chrysalis meal 5～15 parts, Rhizoma Arisaematis 5～15 parts, DIGEN 5～15 parts, Radix Rehmanniae Preparata 10～20 parts in described Chinese herbal feed additive, prolong grass 15～25 parts, Radix et Rhizoma Rhei Franzenbachii 5～15 parts, Radix Angelicae Sinensis 15～25 parts, Fructus Trichosanthis 10～20 parts, white 10～20 parts of rattan of slurry and Radix Buddlejae Butterflybush 5～15 parts.
2. the ricefield el fodder strengthening immunity as claimed in claim 1, it is characterised in that: the weight portion respectively choline chloride 1 part of each raw material, Sal 4 parts, calcium hydrogen phosphate 2 parts, gluten meal 35 parts, corn starch 10 parts, 2 parts of Limnodrilus hoffmeisteri powder, compound vitamin additive 1 part, 25 parts of roe powder, composite mineral matter additive 0.5 part, puer tea slag 0.05 part, esterification vitamin C 15 parts, Chinese herbal feed additive 3 parts in the described ricefield el fodder strengthening immunity.
3. the as claimed in claim 1 ricefield el fodder strengthening immunity, it is characterised in that: the weight portion of each crude drug respectively Hippocampus 28～32 parts, Rhizoma Dioscoreae 23～27 parts, Radix Adenophorae 18～22 parts, cloud roller 18～22 parts, Fructus Crataegi 13～17 parts, white Rhizoma Curculiginis 13～17 parts, dried silkworm chrysalis meal 8～12 parts, Rhizoma Arisaematis 8～12 parts, DIGEN 8～12 parts, Radix Rehmanniae Preparata 13～17 parts in described Chinese herbal feed additive, prolong grass 18～22 parts, Radix et Rhizoma Rhei Franzenbachii 8～12 parts, Radix Angelicae Sinensis 18～22 parts, Fructus Trichosanthis 13～17 parts, white 13～17 parts of rattan of slurry and Radix Buddlejae Butterflybush 8～12 parts.
4. the as claimed in claim 1 ricefield el fodder strengthening immunity, it is characterised in that: the weight portion of each crude drug respectively hippocampus 30 part, Rhizoma Dioscoreae 25 parts, Radix Adenophorae 20 parts, cloud roller 20 parts, Fructus Crataegi 15 parts, white Rhizoma Curculiginis 15 parts, dried silkworm chrysalis meal 10 parts, Rhizoma Arisaematis 10 parts, DIGEN 10 parts, Radix Rehmanniae Preparata 15 parts in described Chinese herbal feed additive, prolong grass 20 parts, Radix et Rhizoma Rhei Franzenbachii 10 parts, Radix Angelicae Sinensis 20 parts, Fructus Trichosanthis 15 parts, white 15 parts of rattan of slurry and Radix Buddlejae Butterflybush 10 parts.
5. the preparation method of the arbitrary described ricefield el fodder strengthening immunity of Claims 1-4, it is characterised in that including:
The first step, weighs gluten meal, roe powder, esterification vitamin C, corn starch in proportion, pulverizes, cross 45-55 mesh sieve, obtain ground product after mix homogeneously；
Second step, weigh choline chloride, Sal, calcium hydrogen phosphate, Limnodrilus hoffmeisteri powder, compound vitamin additive, composite mineral matter additive, puer tea slag, Chinese herbal feed additive in proportion, it is mixed with the ground product obtained by the first step to uniformly, obtain raw mixture；
3rd step, carries out modifier treatment by the raw mixture obtained by second step, and refining temperature is 70-75 DEG C, and the modified humidity to raw mixture is 18-22%；
4th step, sends the raw mixture after the 3rd step matter into feed-processing equipment and is processed into granular mixed feed；
5th step, dries the granular mixed feed of the 4th step gained under 85 DEG C of conditions, is packaged into bag after being subsequently cooled to room temperature.
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| CN107319108A (en) * | 2017-08-01 | 2017-11-07 | 芜湖市木子生态园有限公司 | A kind of ricefield el fodder for suppressing harmful bacteria |
| CN108812449A (en) * | 2018-05-25 | 2018-11-16 | 佛山推启农业研究院（普通合伙） | A kind of cultural method improving swamp eel meat |
| CN108812526A (en) * | 2018-05-25 | 2018-11-16 | 佛山推启农业研究院（普通合伙） | A kind of cultural method improving common eel meat |
| CN108936071A (en) * | 2018-06-26 | 2018-12-07 | 珠海容川饲料有限公司 | A kind of special compound feed and its preparation method and application hybridized after raw fish female parent breeding |
| CN109043227A (en) * | 2018-07-26 | 2018-12-21 | 黄冈市陈绩水产品养殖有限公司 | A kind of feed and preparation method thereof enhancing swamp eel fingerling immunity |
| CN115736096A (en) * | 2022-11-22 | 2023-03-07 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Preparation method and application of seaweed feed |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107319108A (en) * | 2017-08-01 | 2017-11-07 | 芜湖市木子生态园有限公司 | A kind of ricefield el fodder for suppressing harmful bacteria |
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| CN108812526A (en) * | 2018-05-25 | 2018-11-16 | 佛山推启农业研究院（普通合伙） | A kind of cultural method improving common eel meat |
| CN108936071A (en) * | 2018-06-26 | 2018-12-07 | 珠海容川饲料有限公司 | A kind of special compound feed and its preparation method and application hybridized after raw fish female parent breeding |
| CN109043227A (en) * | 2018-07-26 | 2018-12-21 | 黄冈市陈绩水产品养殖有限公司 | A kind of feed and preparation method thereof enhancing swamp eel fingerling immunity |
| CN115736096A (en) * | 2022-11-22 | 2023-03-07 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Preparation method and application of seaweed feed |
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