CN105410344A - Mixed feed for promoting portunus trituberculatus ovarian development and vitellogenesis and preparation method of mixed feed - Google Patents
Mixed feed for promoting portunus trituberculatus ovarian development and vitellogenesis and preparation method of mixed feed Download PDFInfo
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- CN105410344A CN105410344A CN201510947463.3A CN201510947463A CN105410344A CN 105410344 A CN105410344 A CN 105410344A CN 201510947463 A CN201510947463 A CN 201510947463A CN 105410344 A CN105410344 A CN 105410344A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses mixed feed for promoting portunus trituberculatus ovarian development and vitellogenesis and a preparation method of the mixed feed. The mixed feed is characterized in that the mixed feed is prepared by mixing the following raw materials in parts by weight: 25 to 30 parts of fish meal, 20 to 25 parts of soybean meal, 5 to 10 parts of krill meal, 20 to 25 parts of squid viscera meal, 15 to 20 parts of flour, 3 to 5 parts of soya bean lecithin, 1 to 2 parts of fish oil, 0.3 to 0.5 parts of cholesterol, 0.3 to 0.5 parts of choline chloride, 1.5 to 2.5 parts of sodium alginate, 0.5 to 1.0 part of composite additive, 0.5 to 1.0 parts of vitamin mixture, 1.0 to 1.5 parts of mineral substance mixture, 1.0 to 1.5 parts of monocalcium phosphate and 25 to 30 parts of water, the mixed feed has the advantages that the survival rate of portunus trituberculatus is improved by 18 to 22 percent, the gonad index can be improved by 34 to 40 percent, the content of vitellogenin can be improved by 36 to 46 percent, and the molecule expression quantity of the vitellogenin can be improved by 60 to 80 percent.
Description
Technical field
The present invention relates to the mixed feed of Portunus trituberculatus Miers, especially relate to a kind of mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur that promotes.
Background technology
In Portunus trituberculatus Miers breeding process, many employing low-value small fish and shellfish are are directly thrown something and fed, and not only fresh food disparities between supply and demand become increasingly conspicuous, and very easily cause Cultivated water environmental pollution, and disease takes place frequently.More outstanding, the problem that the qualities such as ovarian dysgenesis has appearred in cultivation Portunus trituberculatus Miers, and crab cream color and luster is thin out (or not red) obviously, the coefficient of condition of muscle and crab cream difference decline and the phenomenon such as egg load is few and Offspring's deyelopment is bad.There is (vitellogenesis) and refer to the formation of Yolk and the accumulation in egg mother cell thereof such as various protein, lipid, carbohydrate in yolk, the prerequisite of Oocyte Development maturation, its rolling up with the growth of ovary as feature with livetin original content in hemolymph.Yolk occurs also have decisive role for the cyclostage of animal, it is the most important factor that life is multiplied, the yolk of oocyte is formed as follow-up ontogeny provides material and the energy, the synthesis of yolk, gather, number, distribution and component etc. to its embryo and the young most important.Domestic and international research shows, nutrition is the key factor determining that shrimp crab parent gonad development and yolk occur.For this reason, further investigation Portunus trituberculatus Miers yolk mechanism, seeks the Nutritional solutions of the ovarian dysgenesis of cultivation Portunus trituberculatus Miers, becomes the only way addressed this problem.
The Portunus trituberculatus Miers development of ovary III-IV phase, be ovary Rapid development phase and yolk synthesis most productive period, this stage needs the nutritional supplementation of high-quality abundance, and just can make follow-up crab cream grossness, filial generation quality is significantly improved.China national intellectual property board web discloses and a kind ofly promotes the mixed feed of Portunus trituberculatus Miers female physical culture cream and preparation method thereof (CN201310715226.5) and a kind of swimming crab Yuhong ointment for curing bone disease feed and preparation method thereof (CN201310438934.9).Cream is educated before above-mentioned patent increases color and luster material by adding carrotene, astaxanthin, red algae powder etc. in mixed feed carrys out short-term strengthening listing, the angle that not biochemical from nutrition physiology and yolk is formed, consider nutritive value and the mouthfeel of crab cream, and the development of ovary of swimming crab crucial cyclostage and follow-up laying quality.Also do not disclose any about promoting the mixed feed that the Portunus trituberculatus Miers development of ovary of this stage and yolk occur at present both at home and abroad.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of crab cream quality and promotes mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur and preparation method thereof preferably, this mixed feed makes Portunus trituberculatus Miers survival rate improve 18-22%, genital gland indices can improve 34-40%, vitellogenin content can improve 36-46%, and vitellogenin developed by molecule amount can improve 60-80%.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of mixed feed promoting the Portunus trituberculatus Miers development of ovary and yolk generation, formulated by the raw material Homogeneous phase mixing of following portions by weight: fish meal 25-30; Dregs of beans 20-25; Krill meal 5-10; Squid visceral meal 20-25; Flour 15-20; Soybean lecithin 3-5; Fish oil 1-2; Cholesterol 0.3-0.5; Choline Chloride 0.3-0.5; Sodium alginate 1.5-2.5; Compound additive 0.5-1.0; Vitamin mixtures 0.5-1.0; Mineral mixture 1.0-1.5; Calcium dihydrogen phosphate 1.0-1.5; Water 25-30.
Compound additive described in every kg consists of: DHA-EE oil 200g, EPA-EE oil 100g, Vitwas E 130g, cholesterol 80g, glycerolphosphocholine 30g, astaxanthin 20g, isoflavones 20g, fruit of glossy privet 20g, Radix Astragali 20g, dihydropyridine 30g, seawood meal 250g, hang razor clam powder 100g.
Vitamin mixtures described in every kg consists of: vitamin B12 2.50g, vitamin B2 21.50g, vitamin B6 48.0g, vitamin B12 0.1g, vitamin K5 .5g, vitamin e1 39.0g, VAC 9.5g, Vitamin D5 2g, vitamin B5 103.0g, vitamin B3 59.0g, VB7 23.0g, VB11 6.35g, inositol 157.08g, carrier is cornstarch 353.47g.
Mineral mixture described in every kg consists of: ironic citrate 4g, Zinc vitriol 28g, bitter salt 50g, Manganous sulfate monohydrate 10g, Salzburg vitriol 8g, CoCL2 6H2O 1g, KI 0.78g, potassium dihydrogen phosphate 260g, sodium dihydrogen phosphate 120g, calcium lactate 10g, sodium selenite 0.02g, carrier is zeolite powder 508.2g.
Preferably formulated by the raw material Homogeneous phase mixing of following portions by weight: fish meal 28, dregs of beans 22, krill meal 5, squid visceral meal 16.7, flour 15, soybean lecithin 4, fish oil 2, cholesterol 0.4, Choline Chloride 0.4, sodium alginate 2, compound additive 0.8, vitamin mixtures 0.8, mineral mixture 1.2, calcium dihydrogen phosphate 1.2; Water 28.
The preparation method of the mixed feed that the development of ovary of above-mentioned promotion Portunus trituberculatus Miers and yolk occur, comprises the following steps:
(1) each feedstuff fish meal, dregs of beans, krill meal, squid visceral meal, flour, cholesterol, Choline Chloride, sodium alginate, vitamin mixtures, mineral mixture, calcium dihydrogen phosphate were pulverized 60-80 mesh sieve, gained solid constituent was mixed by following parts by weight: fish meal 25-30; Dregs of beans 20-25; Krill meal 5-10; Squid visceral meal 20-25; Flour 15-20; Soybean lecithin 3-5; Fish oil 1-2; Cholesterol 0.3-0.5; Choline Chloride 0.3-0.5; Sodium alginate 1.5-2.5; Compound additive 0.5-1.0; Vitamin mixtures 0.5-1.0; Mineral mixture 1.0-1.5; Calcium dihydrogen phosphate 1.0-1.5;
(2) in the mixture of step (1) gained, the liquid component of following parts by weight is added: fish oil 1-2; Soybean lecithin 3-5 and water 25-30 mixes again;
(3) by the mixture of step (2) gained steam pressure be 0.4-0.5Mpa, humidity is 18-22%, temperature modified 150-180 second under being the condition of 90-95 DEG C;
(4) be 130-135 DEG C by the mixture of modified for step (3) rear gained in temperature, pressure is 2.5-3Mpa, time is slaking under the condition of 90-120 second, is finally squeezed into the feed that particle diameter is 2-3mm, is namely promoted the mixed feed of the Portunus trituberculatus Miers development of ovary and yolk generation.
Compound additive described in every kg consists of: DHA-EE oil 200g, EPA-EE oil 100g, Vitwas E 130g, cholesterol 80g, glycerolphosphocholine 30g, astaxanthin 20g, isoflavones 20g, fruit of glossy privet 20g, Radix Astragali 20g, dihydropyridine 30g, seawood meal 250g, hang razor clam powder 100g.
Vitamin mixtures described in every kg consists of: vitamin B12 2.50g, vitamin B2 21.50g, vitamin B6 48.0g, vitamin B12 0.1g, vitamin K5 .5g, vitamin e1 39.0g, VAC 9.5g, Vitamin D5 2g, vitamin B5 103.0g, vitamin B3 59.0g, VB7 23.0g, VB11 6.35g, inositol 157.08g, carrier is cornstarch 353.47g.
Mineral mixture described in every kg consists of: ironic citrate 4g, Zinc vitriol 28g, bitter salt 50g, Manganous sulfate monohydrate 10g, Salzburg vitriol 8g, CoCL2 6H2O 1g, KI 0.78g, potassium dihydrogen phosphate 260g, sodium dihydrogen phosphate 120g, calcium lactate 10g, sodium selenite 0.02g, carrier is zeolite powder 508.2g.
Preferably formulated by the raw material of following portions by weight: fish meal 28, dregs of beans 22, krill meal 5, squid visceral meal 16.7, flour 15, soybean lecithin 4, fish oil 2, cholesterol 0.4, Choline Chloride 0.4, sodium alginate 2, compound additive 0.8, vitamin mixtures 0.8, mineral mixture 1.2, calcium dihydrogen phosphate 1.2; Water 28.
Compared with prior art, the invention has the advantages that: the present invention is a kind of promotes mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur and preparation method thereof, this mixed feed adds squid visceral meal, krill meal, lecithin can meet the Portunus trituberculatus Miers ovary Rapid development phase and the nutritional need in yolk synthesis most productive period, significantly improve the development of ovary and yolk generation, improve survival rate, improve growth, strengthen nutritive value and the color and luster local flavor of crab cream, also can improve egg laying amount, the hatchability of fertile eggs of follow-up Portunus trituberculatus Miers parent crab; This mixed feed adds compound additive, this additive according to Growth of Portunus Trituberculatus and reproduction period nutrition physiology feature, by hormone induction and nutriment strengthening, promote the conversion of nutriment in swimming crab body, the development of ovary and the yolk that accelerate swimming crab occur.Raw material wherein selected by compound additive: EPA and DHA can not synthesize or synthetic quantity deficiency in crab body, and the interpolation of this composition significantly can promote the development of ovary of crab, improves Larval quality, has the effect of antiallergy and immunity; Vitamin E has oxidation resistant effect, and its hydrolysate is tocopherol, and can promote that sex hormone is secreted, female hormone concentration increases, and improves fecundity, prevention of miscarriage; Cholesterol can promote growth and the shell of crustacean, be the important source material manufacturing hormone, and hormone is the signal of interest material of the generation of induced animal yolk and gonad development; Phosphatid ylcholine is lipid material main in swimming crab yolk, is composition main in lecithin; Astaxanthin is a kind of a kind of natural colouring matter be present in the biologies such as Crustaceans, green alga and rhodotorula, belongs to the one of carotenoid, can significantly improve gloss and the mouthfeel of crab cream; Isoflavones is by the effective ingredients in plant extracted in crude soya bean, because it and estrogenic molecular structure are closely similar, can combine with ERs, induction gonad development and yolk occur, isoflavones does not have the toxic and side effect of exogenous estrogen, is easy to decompose, and can not pile up in vivo; The fruit of glossy privet contains estrogen-like product, and follicle number is increased, and has stronger specific immunity and nospecific immunity effect; The Radix Astragali, containing saponin, sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper etc., has enhanced machine body immunity function and anti-stress effect; Dihydropyridine has some effect of natural VE, the important regulating and controllings such as Thyroid Hormones In Serum, follicle-stimulating hormone (FSH) element, metakentrin can be improved and produce hormonal readiness, thus improve growth of animal, production (yolk occur, lay eggs) and reproductive performance, improve animal product quality, enhancing function of immune system; Seawood meal can improve the trophic structure of feed, improves efficiency of feed utilization, regulates the organism metabolism of cultivated animals, improves immunity and premunition, growth promoting effects; Razor clam powder of hanging contains the nutriments such as abundant high unsaturated fatty acid, also has stronger food calling effect.In this mixed feed, the each nutrition composition added has synergy, vitamin E and dihydropyridine are worked in coordination with mutually, astaxanthin and seawood meal are supplemented mutually, mutually promote between lipids and hormonal substance, have and significantly improve growth, promote effect that the Portunus trituberculatus Miers development of ovary and yolk occur.
In sum, the present invention promotes the mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur, under the coordinated effect of each composition, Portunus trituberculatus Miers survival rate is made to improve 18-22%, genital gland indices can improve 34-40%, vitellogenin content and developed by molecule amount can improve 36-46%, 60-80% respectively, and average weight can improve 20-25%, and cultured output improves 20-22%.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
One, specific embodiment
Promote the mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur, formulated by the raw material Homogeneous phase mixing of following portions by weight: fish meal 25-30; Dregs of beans 20-25; Krill meal 5-10; Squid visceral meal 15-20; Flour 15-20; Soybean lecithin 3-5; Fish oil 1-2; Cholesterol 0.3-0.5; Choline Chloride 0.3-0.5; Sodium alginate 1.5-2.5; Compound additive 0.5-1.0; Vitamin mixtures 0.5-1.0; Mineral mixture 1.0-1.5; Calcium dihydrogen phosphate 1.0-1.5; Water 25-30.Its preparation method comprises the following steps:
(1) each feedstuff was pulverized 60-80 mesh sieve, gained solid constituent was mixed by above-mentioned parts by weight;
(2) in the mixture of step (1) gained, the liquid component of following parts by weight is added: fish oil 1-2; Soybean lecithin 3-5 and water 25-30 mixes again;
(3) by the mixture of step (2) gained steam pressure be 0.4-0.5Mpa, humidity is 18-22%, temperature modified 150-180 second under being the condition of 90-95 DEG C;
(4) be 130-135 DEG C by the mixture of modified for step (3) rear gained in temperature, pressure is 2.5-3Mpa, time is slaking under the condition of 90-120 second, is finally squeezed into the feed that particle diameter is 2-3mm, is namely promoted the mixed feed of the Portunus trituberculatus Miers development of ovary and yolk generation.
Embodiment 1
Promote to be made up of the mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur: fish meal 28, dregs of beans 22, krill meal 5, squid visceral meal 16.7, flour 15, soybean lecithin 4, fish oil 2, cholesterol 0.4, Choline Chloride 0.4, sodium alginate 2, compound additive 0.8, vitamin mixtures 0.8, mineral mixture 1.2, calcium dihydrogen phosphate 1.2 following component and parts by weight; Water 28, wherein compound additive composition (g/kg pre-mixing agent) is: DHA-EE oil 200g, EPA-EE oil 100g, Vitwas E 130g, cholesterol 80g, glycerolphosphocholine 30g, astaxanthin 20g, isoflavones 20g, fruit of glossy privet 20g, Radix Astragali 20g, dihydropyridine 30g, seawood meal 250g, hang razor clam powder 100g; Vitamin mixtures composition (g/kg pre-mixing agent) is: vitamin B12 2.50g, vitamin B2 21.50g, vitamin B6 48.0g, vitamin B12 0.1g, vitamin K5 .5g, vitamin e1 39.0g, VAC 9.5g, Vitamin D5 2g, vitamin B5 103.0g, vitamin B3 59.0g, VB7 23.0g, VB11 6.35g, inositol 157.08g, carrier is cornstarch 353.47g; Mineral mixture composition (g/kg pre-mixing agent) is: ironic citrate 4g, Zinc vitriol 28g, bitter salt 50g, Manganous sulfate monohydrate 10g, Salzburg vitriol 8g, CoCL2 6H2O 1g, KI 0.78g, potassium dihydrogen phosphate 260g, sodium dihydrogen phosphate 120g, calcium lactate 10g, sodium selenite 0.02g, carrier is zeolite powder 508.2g.Its preparation method comprises the following steps:
(1) each feedstuff was pulverized 80 mesh sieves, gained solid constituent was mixed by following parts by weight: fish meal 28, dregs of beans 22, krill meal 5, squid visceral meal 16.7, flour 15, cholesterol 0.4, Choline Chloride 0.4, sodium alginate 2, compound additive 0.8, vitamin mixtures 0.8, mineral mixture 1.2, calcium dihydrogen phosphate 1.2;
(2) in the mixture of step (1) gained, the liquid component of following parts by weight is added: fish oil 2; Soybean lecithin 4 and water 28 mix again;
(3) by the mixture of step (2) gained steam pressure be 0.4Mpa, humidity is 20%, temperature under being the condition of 90 DEG C modified 150 seconds;
(4) be 130 DEG C by the mixture of modified for step (3) rear gained in temperature, pressure is 2.5Mpa, and the time is slaking under the condition of 90 seconds, is finally squeezed into the feed that particle diameter is 3mm.
Embodiment 2
Promote to be made up of the mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur: fish meal 25, dregs of beans 25, krill meal 8, squid visceral meal 20, flour 18, soybean lecithin 3, fish oil 1.5, cholesterol 0.5, Choline Chloride 0.5, sodium alginate 1.5, compound additive 0.5, vitamin mixtures 1.0, mineral mixture 1.0, calcium dihydrogen phosphate 1.0 following component and parts by weight; Water 25; Wherein compound additive composition (g/kg pre-mixing agent) is: DHA-EE oil 200g, EPA-EE oil 100g, Vitwas E 130g, cholesterol 80g, glycerolphosphocholine 30g, astaxanthin 20g, isoflavones 20g, fruit of glossy privet 20g, Radix Astragali 20g, dihydropyridine 30g, seawood meal 250g, hang razor clam powder 100g; Vitamin mixtures composition (g/kg pre-mixing agent) is: vitamin B12 2.50g, vitamin B2 21.50g, vitamin B6 48.0g, vitamin B12 0.1g, vitamin K5 .5g, vitamin e1 39.0g, VAC 9.5g, Vitamin D5 2g, vitamin B5 103.0g, vitamin B3 59.0g, VB7 23.0g, VB11 6.35g, inositol 157.08g, carrier is cornstarch 353.47g; Mineral mixture composition (g/kg pre-mixing agent) is: ironic citrate 4g, Zinc vitriol 28g, bitter salt 50g, Manganous sulfate monohydrate 10g, Salzburg vitriol 8g, CoCL2 6H2O 1g, KI 0.78g, potassium dihydrogen phosphate 260g, sodium dihydrogen phosphate 120g, calcium lactate 10g, sodium selenite 0.02g, carrier is zeolite powder 508.2g.Its preparation method comprises the following steps:
(1) each feedstuff was pulverized 60 mesh sieves, gained solid constituent was mixed by following parts by weight: fish meal 25, dregs of beans 25, krill meal 8, squid visceral meal 20, flour 18, cholesterol 0.5, Choline Chloride 0.5, sodium alginate 1.5, compound additive 0.5, vitamin mixtures 1.0, mineral mixture 1.0, calcium dihydrogen phosphate 1.0;
(2) in the mixture of step (1) gained, the liquid component of following parts by weight is added: fish oil 1.5; Soybean lecithin 3 and water 25 mix again;
(3) by the mixture of step (2) gained steam pressure be 0.4Mpa, humidity is 21%, temperature under being the condition of 92 DEG C modified 160 seconds;
(4) be 130 DEG C by the mixture of modified for step (3) rear gained in temperature, pressure is 2.5Mpa, and the time is slaking under the condition of 90 seconds, is finally squeezed into the feed that particle diameter is 3mm.
Embodiment 3
Promote to be made up of the mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur: fish meal 30, dregs of beans 20, krill meal 10, squid visceral meal 15, flour 20, soybean lecithin 5, fish oil 1, cholesterol 0.3, Choline Chloride 0.3, sodium alginate 2.5, compound additive 1.0, vitamin mixtures 0.5, mineral mixture 1.5, calcium dihydrogen phosphate 1.5 following component and parts by weight; Water 30; Wherein compound additive composition (g/kg pre-mixing agent) is: DHA-EE oil 200g, EPA-EE oil 100g, Vitwas E 130g, cholesterol 80g, glycerolphosphocholine 30g, astaxanthin 20g, isoflavones 20g, fruit of glossy privet 20g, Radix Astragali 20g, dihydropyridine 30g, seawood meal 250g, hang razor clam powder 100g; Vitamin mixtures composition (g/kg pre-mixing agent) is: vitamin B12 2.50g, vitamin B2 21.50g, vitamin B6 48.0g, vitamin B12 0.1g, vitamin K5 .5g, vitamin e1 39.0g, VAC 9.5g, Vitamin D5 2g, vitamin B5 103.0g, vitamin B3 59.0g, VB7 23.0g, VB11 6.35g, inositol 157.08g, carrier is cornstarch 353.47g; Mineral mixture composition (g/kg pre-mixing agent) is: ironic citrate 4g, Zinc vitriol 28g, bitter salt 50g, Manganous sulfate monohydrate 10g, Salzburg vitriol 8g, CoCL2 6H2O 1g, KI 0.78g, potassium dihydrogen phosphate 260g, sodium dihydrogen phosphate 120g, calcium lactate 10g, sodium selenite 0.02g, carrier is zeolite powder 508.2g.Its preparation method comprises the following steps:
(1) each feedstuff was pulverized 70 mesh sieves, gained solid constituent was mixed by following parts by weight: fish meal 30, dregs of beans 20, krill meal 10, squid visceral meal 15, flour 20, cholesterol 0.3, Choline Chloride 0.3, sodium alginate 2.5, compound additive 1.0, vitamin mixtures 0.5, mineral mixture 1.5, calcium dihydrogen phosphate 1.5;
(2) in the mixture of step (1) gained, the liquid component of following parts by weight is added: fish oil 1; Soybean lecithin 5 and water 30 mix again;
(3) by the mixture of step (2) gained steam pressure be 0.4Mpa, humidity is 22%, temperature under being the condition of 95 DEG C modified 170 seconds;
(4) be 130 DEG C by the mixture of modified for step (3) rear gained in temperature, pressure is 2.5Mpa, and the time is slaking under the condition of 90 seconds, is finally squeezed into the feed that particle diameter is 3mm.
Two, Application Example
Breed variety: average weight 130 grams of Portunus trituberculatus Miers 600 tails, Random assignment is 4 groups, often organizes 3 repetition, and each cement pit (specification is 7.5m × 4m × 1m, long × wide × dark) cultivates Portunus trituberculatus Miers 50, and it is identical to test each group of original body mass; Cultivating condition is as substantially identical in environmental factor, chemical factor and biological factor.
Aquaculture management: other condition is substantially identical, different just cement pit 1 to throw something and feed every day the mixed feed of Portunus trituberculatus Miers of embodiment 1; Cement pit 2 to be thrown something and fed the mixed feed of Portunus trituberculatus Miers of embodiment 2 every day; Cement pit 3 to be thrown something and fed the mixed feed of Portunus trituberculatus Miers of embodiment 3 every day; Cement pit 4 to be thrown something and fed biological feed every day;
Results contrast: after cultivating February, be calculated to be motility rate and rate of body weight gain.Every cement pit gets the consistent swimming crab of 10-15 specification, dissects and obtains ovary, hepatopancrease and muscle, statistics hepatopancreatic cell and ovarian index, measure the average molecular expression of vitellogenin in hemolymph vitellogenin content and ovary after blood drawing.Genital gland indices (GSI): GSI=(weightofgonad/bodyweight) × 100.Vitellogenin content adopts kit to detect (source, Shanghai leaf), and unit is ng/ml; The total serum IgE of the ovary sample of each group adopts Trizol to carry out extracting (the full formula gold in Beijing), reverse transcription adopts First-StrandSynthesisSystemforRT-PCRKit (the full formula gold in Beijing), real-time fluorescence quantitative PCR all adopts Roche Holding Ag's kit (Roche, Switzerland) to carry out.
Experimental result is as shown in table 1.As can be seen from Table 1, cement pit 1,2,3 survival rate ratio throw something and feed biological feed group improve 18-22%, genital gland indices improve 34-40%, vitellogenin content and developed by molecule amount can improve 36-46%, 60-80% respectively, average weight improve 20-25%.Illustrate that the mixed feed of Portunus trituberculatus Miers of the present invention can significantly ovary development accelerating and yolk occur, and the survival rate of Portunus trituberculatus Miers can be improved and accelerate the advantage of Growth of Portunus Trituberculatus speed.
The Growth of Portunus Trituberculatus performance of each cement pit of table 1 and hemolymph vitellogenin content
Certainly, above-mentioned explanation is not limitation of the present invention, and the present invention is also not limited to above-mentioned citing.Those skilled in the art are in essential scope of the present invention, and the change made, remodeling, interpolation or replacement, also should belong to protection scope of the present invention.
Claims (10)
1. promote to it is characterized in that by the raw material Homogeneous phase mixing of following portions by weight: fish meal 25-30 by the mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur formulated; Dregs of beans 20-25; Krill meal 5-10; Squid visceral meal 20-25; Flour 15-20; Soybean lecithin 3-5; Fish oil 1-2; Cholesterol 0.3-0.5; Choline Chloride 0.3-0.5; Sodium alginate 1.5-2.5; Compound additive 0.5-1.0; Vitamin mixtures 0.5-1.0; Mineral mixture 1.0-1.5; Calcium dihydrogen phosphate 1.0-1.5; Water 25-30.
2. a kind of mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur that promotes according to claim 1, is characterized in that the compound additive described in every kg consists of: DHA-EE oil 200g, EPA-EE oil 100g, Vitwas E 130g, cholesterol 80g, glycerolphosphocholine 30g, astaxanthin 20g, isoflavones 20g, fruit of glossy privet 20g, Radix Astragali 20g, dihydropyridine 30g, seawood meal 250g, hang razor clam powder 100g.
3. a kind of mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur that promotes according to claim 1, it is characterized in that the vitamin mixtures described in every kg consists of: vitamin B12 2.50g, vitamin B2 21.50g, vitamin B6 48.0g, vitamin B12 0.1g, vitamin K5 .5g, vitamin e1 39.0g, VAC 9.5g, Vitamin D5 2g, vitamin B5 103.0g, vitamin B3 59.0g, VB7 23.0g, VB11 6.35g, inositol 157.08g, carrier is cornstarch 353.47g.
4. a kind of mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur that promotes according to claim 1, is characterized in that the mineral mixture described in every kg consists of: ironic citrate 4g, Zinc vitriol 28g, bitter salt 50g, Manganous sulfate monohydrate 10g, Salzburg vitriol 8g, CoCL2 6H2O 1g, KI 0.78g, potassium dihydrogen phosphate 260g, sodium dihydrogen phosphate 120g, calcium lactate 10g, sodium selenite 0.02g, carrier is zeolite powder 508.2g.
5. a kind of mixed feed promoting the Portunus trituberculatus Miers development of ovary and yolk generation according to any one of claim 1-4, is characterized in that by the raw material Homogeneous phase mixing of following portions by weight formulated: fish meal 28, dregs of beans 22, krill meal 5, squid visceral meal 16.7, flour 15, soybean lecithin 4, fish oil 2, cholesterol 0.4, Choline Chloride 0.4, sodium alginate 2, compound additive 0.8, vitamin mixtures 0.8, mineral mixture 1.2, calcium dihydrogen phosphate 1.2; Water 28.
6. a preparation method for the mixed feed of the promotion Portunus trituberculatus Miers according to claim 1 development of ovary and yolk generation, is characterized in that comprising the following steps:
(1) each feedstuff fish meal, dregs of beans, krill meal, squid visceral meal, flour, cholesterol, Choline Chloride, sodium alginate, vitamin mixtures, mineral mixture, calcium dihydrogen phosphate were pulverized 60-80 mesh sieve, gained solid constituent was mixed by following parts by weight: fish meal 25-30; Dregs of beans 20-25; Krill meal 5-10; Squid visceral meal 20-25; Flour 15-20; Soybean lecithin 3-5; Fish oil 1-2; Cholesterol 0.3-0.5; Choline Chloride 0.3-0.5; Sodium alginate 1.5-2.5; Compound additive 0.5-1.0; Vitamin mixtures 0.5-1.0; Mineral mixture 1.0-1.5; Calcium dihydrogen phosphate 1.0-1.5;
(2) in the mixture of step (1) gained, the liquid component of following parts by weight is added: fish oil 1-2; Soybean lecithin 3-5 and water 25-30 mixes again;
(3) by the mixture of step (2) gained steam pressure be 0.4-0.5Mpa, humidity is 18-22%, temperature modified 150-180 second under being the condition of 90-95 DEG C;
(4) be 130-135 DEG C by the mixture of modified for step (3) rear gained in temperature, pressure is 2.5-3Mpa, time is slaking under the condition of 90-120 second, is finally squeezed into the feed that particle diameter is 2-3mm, is namely promoted the mixed feed of the Portunus trituberculatus Miers development of ovary and yolk generation.
7. a kind of preparation method promoting the mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur according to claim 5, is characterized in that the compound additive described in every kg consists of: DHA-EE oil 200g, EPA-EE oil 100g, Vitwas E 130g, cholesterol 80g, glycerolphosphocholine 30g, astaxanthin 20g, isoflavones 20g, fruit of glossy privet 20g, Radix Astragali 20g, dihydropyridine 30g, seawood meal 250g, hang razor clam powder 100g.
8. a kind of preparation method promoting the mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur according to claim 5, it is characterized in that the vitamin mixtures described in every kg consists of: vitamin B12 2.50g, vitamin B2 21.50g, vitamin B6 48.0g, vitamin B12 0.1g, vitamin K5 .5g, vitamin e1 39.0g, VAC 9.5g, Vitamin D5 2g, vitamin B5 103.0g, vitamin B3 59.0g, VB7 23.0g, VB11 6.35g, inositol 157.08g, carrier is cornstarch 353.47g.
9. a kind of preparation method promoting the mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur according to claim 5, is characterized in that the mineral mixture described in every kg consists of: ironic citrate 4g, Zinc vitriol 28g, bitter salt 50g, Manganous sulfate monohydrate 10g, Salzburg vitriol 8g, CoCL2 6H2O 1g, KI 0.78g, potassium dihydrogen phosphate 260g, sodium dihydrogen phosphate 120g, calcium lactate 10g, sodium selenite 0.02g, carrier is zeolite powder 508.2g.
10. a kind of preparation method promoting the mixed feed that the Portunus trituberculatus Miers development of ovary and yolk occur according to any one of claim 6-9, is characterized in that by the raw material of following portions by weight formulated: fish meal 28, dregs of beans 22, krill meal 5, squid visceral meal 16.7, flour 15, soybean lecithin 4, fish oil 2, cholesterol 0.4, Choline Chloride 0.4, sodium alginate 2, compound additive 0.8, vitamin mixtures 0.8, mineral mixture 1.2, calcium dihydrogen phosphate 1.2; Water 28.
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