CN104073485A - 制备用于核酸扩增反应的样品的方法、核酸扩增方法及固相核酸扩增反应用试剂和微芯片 - Google Patents

制备用于核酸扩增反应的样品的方法、核酸扩增方法及固相核酸扩增反应用试剂和微芯片 Download PDF

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CN104073485A
CN104073485A CN201410108292.0A CN201410108292A CN104073485A CN 104073485 A CN104073485 A CN 104073485A CN 201410108292 A CN201410108292 A CN 201410108292A CN 104073485 A CN104073485 A CN 104073485A
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nucleic acid
acid amplification
amplification reaction
solid
reagent
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中村友彦
町田贤三
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Sony Corp
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Sony Corp
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201410108292.0A 2013-03-29 2014-03-21 制备用于核酸扩增反应的样品的方法、核酸扩增方法及固相核酸扩增反应用试剂和微芯片 Pending CN104073485A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2013-075428 2013-03-29
JP2013075428A JP5904153B2 (ja) 2013-03-29 2013-03-29 核酸増幅反応用試料の調製方法、核酸増幅方法、固相状核酸増幅反応用試薬及びマイクロチップ

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CN104073485A true CN104073485A (zh) 2014-10-01

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US (1) US20140322761A1 (https=)
JP (1) JP5904153B2 (https=)
CN (1) CN104073485A (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110431232A (zh) * 2017-03-13 2019-11-08 东曹株式会社 用于提取和扩增核酸的试剂
WO2023142131A1 (zh) * 2022-01-30 2023-08-03 皮乐迪有限公司 基于引物和酶一体化小球的目标核酸检测方法
WO2023142129A1 (zh) * 2022-01-30 2023-08-03 皮乐迪有限公司 改进的酶小球及其制备方法和用途

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016065192A1 (en) 2014-10-22 2016-04-28 Roka Bioscience, Inc. Compositions and methods for the detection of nucleic acids
JP2016182112A (ja) * 2015-03-25 2016-10-20 東ソー株式会社 ウイルスからの核酸抽出試薬
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
JP6801237B2 (ja) * 2016-04-21 2020-12-16 東ソー株式会社 ウイルスからの核酸抽出および増幅試薬
WO2017189878A1 (en) * 2016-04-27 2017-11-02 Roka Bioscience, Inc. Compositions and methods for the detection of nucleic acids
WO2018168986A1 (ja) * 2017-03-15 2018-09-20 東洋紡株式会社 遺伝子検査方法及び遺伝子検査キット
JP2018201414A (ja) * 2017-06-05 2018-12-27 東ソー株式会社 保存安定性が増した核酸増幅試薬
TW202246523A (zh) * 2021-01-15 2022-12-01 普渡研究基金會 用於病原目標之環介導恆溫擴增(lamp)分析
US20220228226A1 (en) 2021-01-15 2022-07-21 Purdue Research Foundation Loop-mediated isothermal amplification (lamp) analysis for pathogenic targets
EP4308724A4 (en) * 2021-03-19 2025-05-14 Becton, Dickinson and Company ISOTHERMAL AMPLIFICATION OF PATHOGENS
KR102870677B1 (ko) * 2021-12-24 2025-10-15 주식회사 셀랩시스 비접촉식 초음파 장치를 이용하여 지방 조직으로부터 기질혈관분획을 분리하는 방법 및 이에 이용된 비접촉식 초음파 장치
WO2025070634A1 (ja) * 2023-09-29 2025-04-03 京セラ株式会社 核酸増幅方法、検出方法および核酸増幅システム
WO2026018002A1 (en) * 2024-07-18 2026-01-22 Genomic Labs Ltd Method of purifying bacterial or fungal pathogen dna from blood sample

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1240870B (it) * 1990-02-14 1993-12-17 Talent Procedimento per l'estrazione e la purificazione di dna genomico umano
US5705345A (en) * 1991-01-10 1998-01-06 Amersham International Plc Methods and kits for preparing nucleic acids using cyclodextrin
US5550040A (en) * 1993-06-23 1996-08-27 Hoffman-La Roche Inc. Method, reagents and kits for the detection of Neisseria gonorrhoeae
US6153412A (en) * 1998-12-07 2000-11-28 Bioneer Corporation Lyophilized reagent for polymerase chain reaction
CA2562775C (en) * 2004-04-07 2015-08-04 Access Bio, Inc. Nucleic acid detection system
ES2425354T3 (es) * 2004-06-01 2013-10-14 Alere San Diego, Inc. Amplificación por recombinasa-polimerasa
KR20080066727A (ko) * 2005-11-02 2008-07-16 가부시키가이샤 시마즈세이사쿠쇼 Rna의 추출방법 및 rna의 검출방법
US20080175836A1 (en) * 2005-12-09 2008-07-24 Karp Nelson M Immunogenic composition based on conditionally live virion and method for producing the same
JP2007325581A (ja) * 2006-05-12 2007-12-20 Takeda Chem Ind Ltd 性ホルモン依存疾患の予防・治療剤
US20100099149A1 (en) * 2006-10-06 2010-04-22 Dna Genotek Inc. Stabilizing compositions and methods for extraction of ribonucleic acid
JP5034438B2 (ja) * 2006-10-20 2012-09-26 ソニー株式会社 流路系、及びハイブリダイゼーション検出装置
US20110256592A1 (en) * 2008-12-12 2011-10-20 Eurogentec S.A Use of cyclodextrins to improve the specificity, sensitivity and yield of nucleic acid amplification reactions
CA2688174C (en) * 2008-12-19 2018-08-07 F. Hoffmann-La Roche Ag Dry composition of reaction compounds with stabilized polymerase
WO2012045669A1 (en) * 2010-10-04 2012-04-12 Roche Diagnostics Gmbh Method for cell lysis and pcr within the same reaction vessel
SG193315A1 (en) * 2011-03-04 2013-10-30 Kaneka Corp Nucleic acid detection method, and device and kit for use in same
GB201219137D0 (en) * 2012-10-24 2012-12-05 Ge Healthcare Uk Ltd Direct nucleic acid amplification kit, reagent and method

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110431232A (zh) * 2017-03-13 2019-11-08 东曹株式会社 用于提取和扩增核酸的试剂
CN110431232B (zh) * 2017-03-13 2023-10-24 东曹株式会社 用于提取和扩增核酸的试剂
WO2023142131A1 (zh) * 2022-01-30 2023-08-03 皮乐迪有限公司 基于引物和酶一体化小球的目标核酸检测方法
WO2023142129A1 (zh) * 2022-01-30 2023-08-03 皮乐迪有限公司 改进的酶小球及其制备方法和用途

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US20140322761A1 (en) 2014-10-30
JP5904153B2 (ja) 2016-04-13

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Application publication date: 20141001