CN104012739A - Composite functional beverage, preparation method and application thereof - Google Patents
Composite functional beverage, preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a composite functional beverage, and a preparation method and application thereof. The beverage comprises the following raw materials per 1000 ml: 0.20-0.35 g of maca powder, 0.35-0.54 g of coffee powder, 0.70-1.2 g of guarana extract, 0.4-0.7 g of taurine, 0.07-0.1 g of inositol, 0.03-0.05 g of zinc gluconate, 0.01-0.015 g of nicotinamide, 60.75*10<-3>-1.2*10<-3> g of vitamin B, 0.1-1 g of fruit taste regulator, 1.57-2.35 g of carbon dioxide and the balance is water. The preparation method is simple in process and can be applied to industrial production. Ergonomic test results show that the composite functional beverage, with a low cost, has functions of substantial improvement of taste, alleviation of physical fatigue, yang strengthening and tolerance of hypoxia.
Description
Technical field
The present invention relates to food and drink field, be specifically related to a kind of beverage with compound functions that contains pueraria root powder, Guarana extract and its preparation method and application.
Background technology
The health food majority with effects such as alleviating physical fatigues of State Food and Drug Administration and Ministry of Public Health's approval is to make taking American Ginseng, the Radix Astragali, the fruit of Chinese wolfberry, compound amino acid etc. as raw material at present, its raw material is medicine or healthy food material, taking dose also has strict requirement, can not take as bread and cheese.
Agate coffee (Maca; Lepidium meyenii) be Cruciferae separate row Vegetable spp plant; there is the good reputation of " South America ginseng ", contain rich in protein, aliphatic acid, 20 seed amino acids, 8 kinds of vitamins, taurine, plant polyphenol and alkaloid isoreactivity compositions.Regaining one's strength of body, improve fertility, improve anti-ageing ability, the anxiety of releiving, strengthen memory and focus one's attention on aspect have good effect.At present, all kinds of health products that go out taking agate coffee as main material production are promoted rapidly on the state market such as American-European, Japanese, obtain more and more consumers' favor.Therefore, agate coffee is developed to health drink and there are good market prospects.
As patent CN 102429066 A, CN 1390476A, CN 101537034B discloses respectively the preparation of the health food of the alleviating physical fatigue of making taking pueraria root powder as raw material, but in formula used, other raw material is also Chinese medicine or healthy food material, therefore this product all can not be taken as bread and cheese, owing to containing Chinese medicine in formula, product has denseer taste of traditional Chinese medicine, mouthfeel is not by most people is accepted, and cost is higher, effect is single, limit to a great extent applicable popularity, strengthened the complexity using.
The male sex is after entering midlife, and sexual organ and function thereof also fail gradually with advancing age as other organs in body.There is the sex dysfunction taking sexual hypoesthesia, erectile dysfunction etc. as main manifestations, serious contusion male sex's confidence and self-esteem, harm goodwill as between spouses, is the primary hidden danger of the breakdown of a family.But in most of the cases this going down is temporary transient, can recover.Should pass through active treatment, avoid causing permanent expendable pathology.
Body anoxia phenomenon is seen everywhere in our daily life, often betides and climbs the mountain, plateau, runs, plays ball, bears a heavy burden, goes on a hike and the large activity of oxygen demand such as long-time mental labour.Anoxic refers to because of the oxygen supply deficiency of tissue or uses oxygen obstacle, and cause the metabolism of tissue, the pathologic process of function and morphology structure generation ANOMALOUS VARIATIONS, easily produce the symptoms such as dizziness, headache, tinnitus, dim eyesight, tetraparesis be unable, slow in reacting, be a class pathologic process extremely common in clinical various diseases, the life-critical organ anoxics such as the heart, brain are also the major reasons that causes body death.
Society rhythm of life is accelerated, sub-health state crowd increase day by day, there is the symptoms such as function of human body decline, anoxic, fatigue, middle-aging male hypogona dism, greatly affect quality of life and healthy, therefore be badly in need of working out a kind of new function compound coffee beverage, to regulate function of human body, the quality of making the life better, also healthy.
Summary of the invention
For the deficiencies in the prior art, one of object of the present invention aims to provide one can significantly improve mouthfeel, lower-cost beverage with compound functions, and this beverage not only has alleviating physical fatigue function, also has effect of yang invigorating functions and anoxia endurance.
Two of object of the present invention is to provide the preparation method of above-mentioned beverage with compound functions.
Three of object of the present invention is to provide the application of above-mentioned beverage with compound functions.
Realizing one of object of the present invention can be by taking following technical scheme to reach:
A kind of beverage with compound functions, is characterized in that, the every 1000mL of this beverage is made up of following raw material:
Pueraria root powder 0.20-0.35g, ground coffee 0.35-0.54g, Guarana extract 0.70-1.2g, taurine 0.4-0.7g, inositol 0.07-0.1g, zinc gluconate 0.03-0.05g, niacinamide 0.01-0.015g, pyridoxamine 0.75 × 10
-3-1.2 × 10
-3g, fruity conditioning agent 0.1-1g, carbon dioxide 1.57-2.35g, all the other are water.
As preferably, described fruity conditioning agent is one or more the mixing in white granulated sugar, HFCS, honey, citric acid, natrium citricum, tartaric acid, flavoring essence, food coloring.
Beverage with compound functions of the present invention is prepared from pueraria root powder, ground coffee, Guarana extract, taurine, inositol, zinc gluconate, niacinamide, pyridoxamine, wherein:
Agate coffee is the annual of Cruciferae separate row Vegetable spp or 2 years raw herbaceous plant, originate in mountain areas, Andean more than Peru's height above sea level four kms, edible history in South America has had more than 5800 year, traditionally for improving the health, improve fertility, improve sexual function, antidepression, anti-anaemias etc., are called as " Peru's ginseng ".In agate coffee, contain rich in protein, aliphatic acid, 20 seed amino acids, 8 kinds of vitamins, taurine, plant polyphenol and alkaloid isoreactivity compositions.Regaining one's strength of body, improve fertility, improve anti-ageing ability, the anxiety of releiving, strengthen memory and focus one's attention on aspect have good effect.
Coffee-extract is mainly containing natural caffeine, and in tenth century of Christian era, Arabic has found a kind of fruit refreshed, very excited with relief people of having eaten, Here it is coffee bean.The mode of the edible coffee of Arabic is that whole fruit chewed the earliest, draw juice as replenishing agent, completely different through the coffee bean curing from the modern times, the green coffee bean that tradition directly eats does not have the fragrance of coffee, but the natural composition of refreshing oneself obtains larger reservation.Appropriate caffeine also can alleviate muscular fatigue, promotes digestive juice secretion.Coffee can antifatigue, promote metabolism, active digestive organs.
Guarana is the wild certain kind of berries of the one of Amazon special product, is one of the most well-known rainforest medicinal plant of Brazil, and nutritive value is very high.Guarana is planted by Indians the earliest, is called as " god fruit ", and they chew Guarana seed at custom in the time hunting and wrestle, to obtain energy.With Guarana make beverage be regarded as " state's drink " in Brazil.
Taurine is a seed amino acid of needed by human, but the ability of human body self synthesizing taurine is very limited.Taurine participates in glycometabolic adjusting, accelerate glycolysis, be also essential for maintaining locomitivity, strengthen its supplement can make locomitivity and resisting kinetic fatigue ability further strengthen.
Inositol has the effect that maintains the metabolism treatment mechanism of human body to various tired materials, extenuates pressure, supplements energy and muscle power.
Zinc gluconate is dissociated into zinc ion and gluconic acid in vivo, participates in the synthetic of ribonucleic acid and DNA, thereby can promote wound healing, promotes growth, promotes zinc enzyme function in body.Being necessary to human body, is good nutrition fortifier, suitably supplements and can improve body immunity.
Niacinamide is the part of DPN and DPN I, becomes the coenzyme of many dehydrogenases.Niacinamide is combined with adenine, ribose and phosphoric acid in vivo and is formed NADH and nicotinamide-adenine dinucleotide phosphate, in biological oxidation-reduction, play electron carrier or hydrogen carrier effect, the pyridine ring of niacinamide has the characteristic that hydrogenation reversibly adds electronics and the de-electronics of dehydrogenation, so niacinamide participates in energetic supersession and the fundamentally effect of trophic nerve, fundamentally alleviating physical fatigue of supplementary niacinamide, increases energy.
Pyridoxamine participates in the metabolism of protein composition and decomposition, participates in all amino acid metabolisms, as relevant with the metabolism of ferroheme, relevant with the synthetic nicotinic acid of tryptophan, and can strengthen immunity.
Realizing two of object of the present invention can be by taking following technical scheme to reach:
A preparation method for beverage with compound functions, is characterized in that, comprises the following steps:
1) pueraria root powder, ground coffee, Guarana extract are used to hot water dissolving, add with in the fruity conditioning agent after hot water dissolving, filter, obtain filtrate;
2) by taurine, inositol, zinc gluconate, niacinamide, the pyridoxamine water-soluble solution of thermal process, add above-mentioned filtrate, with sterilization after the allotment of fruity conditioning agent, product in the middle of obtaining;
3) middle product are mixed with remaining water, pass into carbon dioxide, filling, sterilization, packs and get final product.
As preferably, step 1) in, described filtration is with filtering with 5 μ filter bags after diatomite filtration again.The present invention is taking diatomite as main medium, utilize trickle property and the porous of diatomite particle not only can remove the impurity such as suspended particulate, colloid in beverage with compound functions, also can keep that the local flavor of product is constant, nontoxic, no suspended substance and sediment, clear, degree of filtration be high.The present invention, with filtering with employing filter bag after diatomite filtration, has further improved filtering accuracy and filter efficiency again, has ensured the quality of product.
As preferably, step 2) in, described sterilization is UHT sterilization, temperature 110-130 DEG C, and the time is 20-30s.The present invention selects UHT sterilization, i.e. the instantaneous processing of superhigh temperature (Ultra High Temperature treated) sterilization, and the heat time is shorter, and less to nutritional labeling destruction, after UHT sterilization, shelf life of products is longer.
As preferably, step 3) in, described sterilization is pasteurize, temperature 81-100 DEG C, and the time is 10-20min.The present invention by pasteurize, utilizes the lower temperature not only can kill pathogens but also can keep in product nutriment local flavor constant after product is filling.
As preferably, step 3) in, described carbon dioxide is passed into pressurization in carbonation apparatus while filling with, and pressure remains within the scope of 0.1MPa-0.2MPa, ensures that in tank, temperature is 3 DEG C-6 DEG C simultaneously.
Realizing three of object of the present invention can be by taking following technical scheme to reach:
Beverage with compound functions has establishing-Yang, alleviating physical fatigue or improves the application in the beverage of anoxia endurance effect in preparation.
Described beverage is one or more in soda, tea beverage, fruit juice and juice beverage, protein beverage, solid beverage, coffee beverage, plant beverage, flavor beverage.
Described beverage is in beverage with compound functions, to add appropriate auxiliary material or additive, make any type of beverage, the kind of described beverage comprises one or several in soda (carbonated drink) class, tea beverage class, fruit juice and vegetable juice class, protein beverage class, solid beverage class or Other Drinks class.Above-mentioned various types of beverage can be according to the conventional method preparation of field of beverage.
Beneficial effect of the present invention is:
Beverage with compound functions of the present invention is taking pueraria root powder, ground coffee, Guarana extract as main composition, its preparation technology is simple, when guarantee product nutrient substances local flavor is constant, significantly improve mouthfeel, adaptation population is extensive, cost is lower etc., is suitable for suitability for industrialized production, verifies through pharmacodynamic experiment, beverage with compound functions of the present invention can shorten flutterring of castration male rat to be caught incubation period, increases to flutter to catch number of times and ejaculation frequency; Can extend the swimming time of mice burden swimming, the content of hepatic glycogen in blood after raising mouse movement, reduce Serum lactic acid content after mouse movement, and can extend the time-to-live of mouse after mouse normal pressure resistance to anoxic incubation period and Ischemia Injury in Brain anoxic, there is effect of obvious nourishing and strengthening vital, alleviating physical fatigue and raising anoxia endurance.
Brief description of the drawings
Fig. 1 is that beverage with compound functions is flutterred castrated rats and caught incubation period, flutters the impact of catching number of times, ejaculation frequency;
Fig. 2 is that beverage with compound functions is flutterred the impact of catching animal number of cases, ejaculation animal number of cases and testosterone on castrated rats;
The impact that Fig. 3 is beverage with compound functions on castrated rats prostate and seminal vesicle, levator ani organ index;
Fig. 4 is that beverage with compound functions is flutterred normal rat and caught incubation period, flutters the impact of catching number of times, ejaculation frequency;
Fig. 5 is that beverage with compound functions is flutterred the impact of catching animal number of cases, ejaculation animal number of cases and testosterone on normal rat;
Fig. 6 is the impact of beverage with compound functions on normal rat prostate, seminal vesicle and levator ani organ index;
Fig. 7 is the impact of beverage with compound functions on the mice burden swimming time;
Fig. 8 is the impact of beverage with compound functions on serum urea nitrogen;
Fig. 9 is the impact of beverage with compound functions on hepatic glycogen;
Figure 10 is the impact of beverage with compound functions on blood lactic acid;
Figure 11 is that beverage with compound functions is on the preclinical impact of the resistance to anoxic of normal pressure;
Figure 12 is the impact of beverage with compound functions on the poisoning time-to-live of natrium nitrosum;
Figure 13 is the impact of beverage with compound functions on Ischemia Injury in Brain the survival time under hypoxic condition.
Detailed description of the invention
Further illustrate the present invention below by detailed description of the invention, following examples are preferably embodiment of the present invention, but embodiments of the present invention are not subject to the restriction of following embodiment.
The raw materials used commercially available prod that is in following embodiment.
Embodiment 1:
A kind of beverage with compound functions, the every 1000mL of this beverage is made up of following raw material:
Pueraria root powder 0.20g, ground coffee 0.35g, Guarana extract 0.70g, taurine 0.4g, inositol 0.07g, zinc gluconate 0.03g, niacinamide 0.01g, pyridoxamine 0.75mg, fruity conditioning agent 0.1g, carbon dioxide 1.57g, all the other are water.
Described fruity conditioning agent is one or more the mixing in white granulated sugar, HFCS, honey, citric acid, natrium citricum, tartaric acid, flavoring essence, food coloring.
The preparation method of described beverage with compound functions, comprises the following steps:
1) by aforementioned proportioning, ground coffee, Guarana extract, pueraria root powder are used to hot water dissolving, add with in the white granulated sugar after hot water dissolving, add HFCS, with filtering with 5 μ filter bags again after diatomite filtration, obtain filtrate;
2) press aforementioned proportioning by the water-soluble solution of taurine, inositol, zinc gluconate, niacinamide, pyridoxamine, citric acid, natrium citricum and tartaric acid thermal process, add above-mentioned filtrate, with UHT sterilization after honey, flavoring essence and food coloring allotment, temperature is 110 DEG C, time is 20s, product in the middle of obtaining;
3) middle product are mixed to (toward the constant volume that adds water in middle product) with remaining water by aforementioned proportioning, pass into carbon dioxide, filling, pasteurize, 81 DEG C of temperature, the time is 10min, packs and get final product.
Embodiment 2:
A kind of beverage with compound functions, the every 1000mL of this beverage is made up of following raw material:
Pueraria root powder 0.28g, ground coffee 0.45g, Guarana extract 0.95g, taurine 0.55g, inositol 0.085g, zinc gluconate 0.04g, niacinamide 0.013g, pyridoxamine 1mg, fruity conditioning agent 0.5g, carbon dioxide 1.96g, all the other are water.
The preparation method of described beverage with compound functions, comprises the following steps:
1) by aforementioned proportioning, ground coffee, Guarana extract, pueraria root powder are used to hot water dissolving, add with in the white granulated sugar after hot water dissolving, add HFCS, with filtering with 5 μ filter bags again after diatomite filtration, obtain filtrate;
2) press aforementioned proportioning by the water-soluble solution of taurine, inositol, zinc gluconate, niacinamide, pyridoxamine, citric acid, natrium citricum and tartaric acid thermal process, add above-mentioned filtrate, with UHT sterilization after honey, flavoring essence and food coloring allotment, temperature is 118 DEG C, time is 25s, product in the middle of obtaining;
3) by aforementioned proportioning, middle product are mixed with remaining water, pass into carbon dioxide, filling, pasteurize, 90 DEG C of temperature, the time is 15min, packs and get final product.
Embodiment 3:
A kind of beverage with compound functions, the every 1000mL of this beverage is made up of following raw material:
Pueraria root powder 0.35g, ground coffee 0.54g, Guarana extract 1.2g, taurine 0.7g, inositol 0.1g, zinc gluconate 0.05g, niacinamide 0.015g, pyridoxamine 1.2mg, fruity conditioning agent 1g, carbon dioxide 2.35g, all the other are water.
The preparation method of described beverage with compound functions, comprises the following steps:
1) by aforementioned proportioning, ground coffee, Guarana extract, pueraria root powder are used to hot water dissolving, add with in the white granulated sugar after hot water dissolving, add HFCS, with filtering with 5 μ filter bags again after diatomite filtration, obtain filtrate;
2) press aforementioned proportioning by the water-soluble solution of taurine, inositol, zinc gluconate, niacinamide, pyridoxamine, citric acid, natrium citricum and tartaric acid thermal process, add above-mentioned filtrate, with UHT sterilization after honey, flavoring essence and food coloring allotment, temperature is 130 DEG C, time is 30s, product in the middle of obtaining;
3) by aforementioned proportioning, middle product are mixed with remaining water, pass into carbon dioxide, filling, pasteurize, 100 DEG C of temperature, the time is 20min, packs and get final product.
Pharmacodynamics test research
Further set forth beneficial effect of the present invention by pharmacodynamics test below.
1. experiment material
1.1 animals used as test: SPF level KM mouse, SPF level SD rat, male and female half and half, animal credit number: SCXK (Guangdong) 2013-0020, animal is purchased from Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center.Routine feeding one week, SPF Ji Shuliaoyou Traditional Chinese Medicine University Of Guangzhou Experimental Animal Center provides.
1.2 experimental situations: Animal House room temperature 20-25 DEG C, humidity 50-70%, experimental situation facility credit number: SYXK (Guangdong) 2008-0092.
30 times of concentrates of 1.3 given the test agent: embodiment, 1 sample, are provided lot number 20130625. quantities by Tiandi No. 1 Drink Co., Ltd.: by 500ml/d, be 16.7ml/d (calculating by concentrate below) after concentrating, normal temperature is preserved.
1.4 instruments and reagent: automatic clinical chemistry analyzer; Full-automatic blood cell analysis instrument; Electronic analytical balance; Automatic water extracter for biological tissue; Biological tissue embedding machine; Micropipettor; Organize dispersion machine; Oestradiol benzoate; Progesterone; Absolute ethyl alcohol; Formaldehyde; Haematoxylin; Glacial acetic acid; Dimethylbenzene; Chloraldurate; Soda lime; Natrium nitrosum; Lactic acid Kit; Physiological saline.
2. test method
2.1 yang invigorating functions checkings
2.1.1 the impact on castration male rat sexual function
Get 75 of SPF level male SD rats, body weight 180-220g, get at random 15 rats as Normal group, 3ml/kg10% chloraldurate intraperitoneal injection of anesthesia for all the other 60 rats, the sterilization of An Er iodine scrotum, extract bilateral testes, after 3 days, modeling rat be divided into 4 groups at random, model group, height (12.5ml/kg/d), in (6.25ml/kg/d), low dosage (3.13ml/kg/d) group.According to dosage gastric infusion of each dosage group, administration volume is 1.25ml/100g; Model group and Normal group give the physiological saline of same volume, and 1 time/d, continuous 30 days.
Get 75 of female sd inbred rats, before mating test, 48h and 4h hypodermic injection oestradiol benzoate 20 μ g and progesterone 500 μ g (dissolving with the sesame oil of 0.1ml respectively) induce female mouse to oestrus respectively.The male mouse of last administration (1h after medicine) is put into and observes cage, adapt to 15min, again the good female mouse that oestruses is put into cage (1:1) gently, observe and record it and in 40min, flutter and catch incubation period, flutter and catch number of times, ejaculation frequency, flutter and catch animal number of cases and ejaculation animal number of cases.Under male rat eye socket veniplex, get blood, measure Testosterone Content in Serum, get the sexual organ such as prostate and seminal vesicle, levator ani and measure weight in wet base, calculate organ index.
2.1.2 the impact on normal male rat sexual function
Get 60 of SPF level male SD rats, body weight 180-220g, be divided at random normal group, height (12.5ml/kg/d), in (6.25ml/kg/d), low dosage (3.13ml/kg/d) group, gastric infusion, 1 time/d, continuous 14 days, respectively organize all same 2.1.1 such as administration, animal mating mode, index observing and mensuration.
2.1.3 data statistics and processing
All experimental datas are added and subtracted standard deviation with mean
represent, adopt one-way analysis of variance, between group, average relatively adopts SNK method, and when heterogeneity of variance, between group, average relatively adopts Dunnett T3 method between two.Above data are carried out statistical analysis, α=0.05 by SPSS15.0.The results are shown in Figure of description 1-6.
2.1.4 interpretation of result
From Figure of description 1, compared with model group, high, medium and low dosage group all can shorten flutterring of castrated rats catches incubation period, increases to flutter to catch number of times and ejaculation frequency, and wherein high dose group and model group relatively have notable statistics difference (P<0.05); From Figure of description 2,3, compared with model group, high, medium and low dosage group all can increase castrated rats flutters and catches animal number of cases and ejaculation animal number of cases, Testosterone Content in Serum, prostate, seminal vesicle and levator ani index, but does not all have significant difference (P>0.05).From Figure of description 4, compared with Normal group, high, medium and low dosage group all can shorten flutterring of castrated rats catches incubation period, increases to flutter to catch number of times and ejaculation frequency, but does not have statistical significance (P>0.05); From Figure of description 5, compared with Normal group, high, medium and low dosage group all can increase rat blood serum testosterone concentration, and high, middle dosage group can also increase ejaculation animal number of cases, but does not have statistical significance (P>0.05); From Figure of description 6, compared with Normal group, high, medium and low dosage group all can improve prostate, seminal vesicle and the levator ani index of castrated rats, but does not have statistical significance (P>0.05).Result of the test shows, beverage with compound functions of the present invention has yang invigorating functions to castration male rat.
In recent years, the health efficacy of agate coffee and goods thereof, the effect that for example raises libido, improves the aspects such as fecundity have been reported in research both at home and abroad.Agate coffee is called as natural " hormone engine ", and bioactivator exclusive in composition, as agate coffee alkene, agate coffee acid amides, can improve sperm quantity and enliven ability; Alkaloid in agate coffee can Hypothalamic Stimulation and pituitary, regulates the functions such as adrenal gland, pancreas, testis, balance hormone level, thus improve male's sexual.With advancing age and the impact of live and work pressure, testicular function reduces gradually, and the property such as sexual hypoesthesia, sexual life indifference appears in most of middle-aging males
The symptoms such as dysfunction.From result of the test, beverage with compound functions of the present invention can shorten flutterring of castrated rats and catch incubation period, increase to flutter and catch number of times and ejaculation frequency, show that it can strengthen castration male rat sexual function, improves significantly to sex dysfunctions such as middle-aging male sexual hypoesthesia, sexual life indifferences.
2.2 effect of relieving physical fatigue checkings
2.2.1 mice burden swimming experiment
60 of SPF level KM mouse, male and female half and half, 18-22g, random packet, be divided into height (27.1mL/kg/d), in (13.55mL/kg/d), low dosage (6.78mL/kg/d) group, Normal group, 15 every group.Each dosage group dosage gastric infusion, administration volume is 0.271mL/10g; Normal group gives the physiological saline of same volume, and 1 time/d, continuous 15 days.After last administration 0.5h, mouse is put into (25 ± 0.5) DEG C water temperature, 30cm depth of water swimming trunk went swimming, ties up the lead of its body weight 5% on mousetail, after mouse sinks under water 6s, do not refloat and exhaust death standard as power.Record mouse and start to the death time Zi swimming, as the swimming time of mouse.
2.2.2 on serum urea nitrogen impact experiment
All same 2.2.1 such as animal grouping and medication, after last administration 0.5h, mouse is put into (30 ± 0.5) DEG C water temperature, 30cm depth of water swimming trunk went swimming, every case is once put into 5 mouse, and not swimming with a load attached to the body 1.5h towels off dry mouse water with it, after rest 1h, pluck eyeball and get blood, centrifuging and taking serum, remaining operation is carried out serum urea nitrogen determination by kit description.
2.2.3 the impact experiment on hepatic glycogen content
All same 2.2.1 such as animal grouping and medication, put to death mouse after last administration 0.5h and get liver, use physiological saline rinsing, and blot with filter paper, and precision takes 200mg liver, and remaining operation carried out hepatic glycogen mensuration by kit description.
2.2.4 the impact experiment on blood lactic acid
All same 2.2.1 such as animal grouping and medication, after last administration 0.5h, mouse is put into (30 ± 0.5) DEG C water temperature, 30cm depth of water swimming trunk went swimming, every case is once put into 5 mouse, and not swimming with a load attached to the body 10 minutes towels off dry mouse water with it, get blood 20 μ L to be measured, after rest 20min, then get blood 20 μ L, remaining operation is carried out blood Plasma lactate by kit description.
2.2.5 data analysis
All experimental datas are added and subtracted standard deviation with mean
represent, adopt one-way analysis of variance, between group, average relatively adopts SNK method, and when heterogeneity of variance, between group, average relatively adopts Dunnett T3 method between two.Above data are carried out statistical analysis, α=0.05 by SPSS15.0.The results are shown in Figure of description 7-10.
2.2.6 interpretation of result
From Figure of description 7, compared with Normal group, high, medium and low dosage group mouse all shows the longer swimming with a load attached to the body time, wherein the difference between high, middle dosage group and Normal group has statistical significance (P<0.05), illustrates that the beverage with compound functions of high dose of the present invention and middle dosage can increase the mice burden swimming time.From Figure of description 8, compared with Normal group, after high, medium and low dosage group mouse movement, the mean value of urea in serum nitrogen level is all a little less than Normal group, but do not there is statistical significance (P>0.05), illustrate that the serum urea nitrogen impact after beverage with compound functions of the present invention is on mouse movement is little.From Figure of description 9, compared with Normal group, after high, medium and low dosage group mouse movement, all there is higher liver hepatic glycogen content, wherein between high dose group and Normal group, there is utmost point significant difference (P<0.01), point out beverage with compound functions high dose group of the present invention to there is the effect that promotes Mouse Liver glycogen deposit.From Figure of description 10, compared with Normal group, after high, medium and low dosage group mouse movement, the content of blood lactic acid is all lower, wherein relatively there were significant differences (P<0.05) for high dose group and Normal group, illustrates that the beverage with compound functions of the present invention of high dose raises and has inhibitory action the blood lactic acid of mouse after moving.With reference to " health food inspection with assessment technique specification " (version in 2003) effect of relieving physical fatigue item standard that judges, show that beverage with compound functions of the present invention has effect of relieving physical fatigue function.
Fatigue is the physiological and biochemical procedure of body complexity, and body consumes glycogen in a large number, thereby causes the generation of whole body fatigue, affects the eubolism process in the relatively stable and body of organismic internal environment.The business people of long-time busy work, consultation service industry personage, need the professional driver of long-duration driving and private car owner, all through the night party leisure personage, sports fan, need to keep the crowds such as the student of learning state very easily to produce fatigue.Along with the increase of exercise load, in blood, urea content increases thereupon, and therefore, urea accumulating and a large amount of consumption of human body energy material in vivo, is a key factor that causes exercise induced fatigue.The important sources of energy when glycogen is motion, its how many direct locomitivities that affect, for this reason, the deposit that improves glycogen is blood sugar level while maintaining motion, contributes to strengthen endurance and locomitivity, is conducive to the generation of resisting fatigue.Beverage with compound functions of the present invention has the function of alleviating physical fatigue, can eliminate in time human-body fatigue symptom, and makeup energy, recovers rapidly human physical power.
2.3 resisting oxygen lack checkings
2.3.1 Resistance to anoxia
All same 2.2.1 such as animal grouping and medication, after last administration 1h, mouse is put into the 250mL port grinding bottle (volume error ± 1mL) that fills 5g soda lime, be coated with vaseline sealing bottleneck, and timing immediately, to cease breathing as index, observe and record the time-to-live (min) of the resistance to anoxic of mouse normal pressure.
2.3.2 the poisoning survival test of natrium nitrosum
All same 2.2.1 such as animal grouping and medication, after last administration 1h by mouse by 230mg/kg lumbar injection natrium nitrosum (injection volume is 10mL/kg), and timing immediately, to cease breathing as index, record the mouse survival time (min).
2.3.3 Ischemia Injury in Brain anoxic survival test
Animal grouping and all same 2.2.1 of administration, break end mouse after last administration 1h from neck, record after mouse breaks end and dehisce the snorting time (min) with stopwatch.
2.3.4 data analysis
All experimental datas are added and subtracted standard deviation with mean
represent, adopt one-way analysis of variance, between group, average relatively adopts SNK method, and when heterogeneity of variance, between group, average relatively adopts Dunnett T3 method between two.Above data are carried out statistical analysis, α=0.05 by SPSS15.0.The results are shown in Figure of description 11-13.
2.3.5 interpretation of result
From Figure of description 11, compared with Normal group, high, medium and low dosage group all can extend the resistance to anoxic of mouse normal pressure incubation period, wherein high, middle dosage group difference compared with Normal group has conspicuousness (P<0.05), illustrates that high, middle dosage can extend the resistance to anoxic of mouse normal pressure incubation period.From Figure of description 12, compared with Normal group, the high, medium and low dosage group mouse poisoning time-to-live of natrium nitrosum is all a little more than Normal group, but do not there is statistical significance (P>0.05), illustrate that beverage with compound functions of the present invention is little on the time-to-live impact of the poisoning rear mouse of natrium nitrosum.From Figure of description 13, compared with Normal group, high, medium and low dosage group all can extend the time-to-live of mouse after Ischemia Injury in Brain anoxic, wherein relatively there were significant differences (P<0.001, P<0.05) for high, middle dosage group and Normal group.The beverage with compound functions that high dose of the present invention and middle dosage is described can extend the time-to-live of mouse after Ischemia Injury in Brain anoxic.Improve the anoxia endurance item standard that judges with reference to " health food inspection with assessment technique specification " (version in 2003), show that beverage with compound functions of the present invention has raising anoxia tolerant function.
Body anoxia phenomenon is seen everywhere in our daily life, often betides and climbs the mountain, plateau, runs, plays ball, bears a heavy burden, goes on a hike and the large activity of oxygen demand such as long-time mental labour.Anoxic can cause the function of body and the change of metabolism state, easily produces the symptoms such as dizziness, headache, tinnitus, dim eyesight, tetraparesis be unable, slow in reacting, is a class pathologic process extremely common in clinical various diseases.The life-critical organ anoxic such as brain, the heart is also the major reason that causes body death.Experiment results proved, beverage with compound functions of the present invention has the function that improves anoxia endurance, can avoid or reduce the damage that histoorgan causes because of anoxic, recovers rapidly organism metabolism function.
Comprehensively above experiment can be found out, beverage with compound functions of the present invention has effect of obvious nourishing and strengthening vital, alleviating physical fatigue and raising anoxia endurance, has larger development prospect.
Above-described embodiment, only for the preferred case study on implementation of the present invention, can not limit the present invention's scope required for protection with this, any unsubstantiality that those skilled in the art does on basis of the present invention and replace and all belong to the scope of protection of present invention.
Claims (9)
1. a beverage with compound functions, is characterized in that, the every 1000mL of this beverage is made up of following raw material:
Pueraria root powder 0.20-0.35g, ground coffee 0.35-0.54g, Guarana extract 0.70-1.2g, taurine 0.4-0.7g, inositol 0.07-0.1g, zinc gluconate 0.03-0.05g, niacinamide 0.01-0.015g, pyridoxamine 0.75 × 10
-3-1.2 × 10
-3g, fruity conditioning agent 0.1-1g, carbon dioxide 1.57-2.35g, all the other are water.
2. beverage with compound functions according to claim 1, is characterized in that: described fruity conditioning agent is one or more the mixing in white granulated sugar, HFCS, honey, citric acid, natrium citricum, tartaric acid, flavoring essence, food coloring.
3. the preparation method of beverage with compound functions according to claim 1 and 2, is characterized in that, comprises the following steps:
1) pueraria root powder, ground coffee, Guarana extract are used to hot water dissolving, add with in the fruity conditioning agent after hot water dissolving, filter, obtain filtrate;
2) by taurine, inositol, zinc gluconate, niacinamide, the pyridoxamine water-soluble solution of thermal process, add above-mentioned filtrate, with sterilization after the allotment of fruity conditioning agent, product in the middle of obtaining;
3) middle product are mixed with remaining water, pass into carbon dioxide, filling, sterilization, packs and get final product.
4. the preparation method of beverage with compound functions according to claim 3, is characterized in that: step 1) in, described filtration is with filtering with 5 μ filter bags after diatomite filtration again.
5. the preparation method of beverage with compound functions according to claim 3, is characterized in that: step 2) in, described sterilization is UHT sterilization, temperature 110-130 DEG C, and the time is 20-30s.
6. the preparation method of beverage with compound functions according to claim 3, is characterized in that: step 3) in, described sterilization is pasteurize, temperature 81-100 DEG C, and the time is 10-20min.
7. the preparation method of beverage with compound functions according to claim 3, it is characterized in that: step 3) in, when described carbon dioxide is passed into the pressurization filling in carbonation apparatus, pressure remains within the scope of 0.1MPa-0.2MPa, ensures that in tank, temperature is 3 DEG C-6 DEG C simultaneously.
8. the beverage with compound functions described in claim 1 or 2 has establishing-Yang, alleviating physical fatigue or improves the application in the beverage of anoxia endurance effect in preparation.
9. application according to claim 8, is characterized in that: described beverage is one or more in soda, tea beverage, fruit juice and juice beverage, protein beverage, solid beverage, coffee beverage, plant beverage, flavor beverage.
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