CN102613653A - Nutrient beverage containing gamma-aminobutyric acid and preparation method of nutrient beverage - Google Patents
Nutrient beverage containing gamma-aminobutyric acid and preparation method of nutrient beverage Download PDFInfo
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- CN102613653A CN102613653A CN2012101127763A CN201210112776A CN102613653A CN 102613653 A CN102613653 A CN 102613653A CN 2012101127763 A CN2012101127763 A CN 2012101127763A CN 201210112776 A CN201210112776 A CN 201210112776A CN 102613653 A CN102613653 A CN 102613653A
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Abstract
The invention discloses a nutrient beverage containing gamma-aminobutyric acid. The nutrient beverage comprises 0.005 to 0.05 percent of gamma-aminobutyric acid, 0.005 to 0.1 percent of composite amino acid, 0.1 to 6.6 percent of fructose corn syrup, 0.01 to 0.1 percent of acesulfame potassium, 0.01 to 0.1 percent of sodium citrate, 0 to 0.01 percent of potassium sorbate, 0.01 to 0.1 percent of flavoring essence and the balance water. The nature health care factor of gamma-aminobutyric acid with the biological activity is compounded and matched with the compound amino acid, the nutrient beverage is delicious, the mouth feeling is good, and in addition, the daily life and work pressure can be effectively relieved. The invention also discloses a preparation method of the nutrient beverage containing the gamma-aminobutyric acid. The preparation method comprises the steps of proportioning, degassing, homogenizing, sterilization, cooling, filling and packing. The industrialized production can be realized through the exiting equipment sold on the market, and the nutrient beverage and the preparation method have the advantages that the preparation is simple, the implementation is easy, the control is easy, the reproducibility is good, and the like.
Description
Technical field
The present invention relates to the nutrients beverage field, particularly a kind of nutrients beverage that contains GABA and preparation method thereof.
Background technology
GABA (english abbreviation GABA) is that a kind of naturally occurring non-albumen is organized amino acid; It is inhibiting nerve mediator important in the mammalian central nervous system; Participate in multiple metabolic activity; Have very high physiologically active, mainly show following several respects: (1) calm neural, antianxiety; (2) bring high blood pressure down; (3) reduce blood ammonia; (4) improve the brain vigor; (5) nerves such as treatment epilepsy, the senile dementia disease of decaying; (6) promote alcohol metabolism.Up-to-date research shows that GABA also has some new physiological functions such as promoting pancreatic secretion insulin, effectively controls diabetic's state of an illness; Improve the synthetic ratio of PGH concentration and brain protein; Suppress source, stingy road property adenocarcinoma of lung; Closely related with the generation of the motion of sperm and steroid hormone, the capacitation spermatogenesis acrosome reaction of bringing out the people, thus regulate male reproductive function etc.
GABA is owing to have very high physiologically active and have many effects, thereby is widely used at field of medicaments.External GABA is commercialization and very fast-selling.Aspect GABA food, health careization, Japan is walked in prostatitis, and major company such as ocean spices, big wound, research institute of chlorella yakult central authorities and mechanism are all at active development of GABA food material and health product.The GABA research and development of products also is devoted in the U.S., Singapore, France and Taiwan.In China, No. 12 bulletin approval of health ministry 2009 GABA is a new resource food, can be used as nutritious supplementary pharmaceutical and use, but domestic GABA commercialization, foodization and health careization relatively lags behind, and GABA food is less relatively.
One Chinese patent application CN 102106381A discloses a kind of milk tea beverage that is rich in GABA and preparation method thereof; To process this milk tea beverage 1000mL, comprise the raw material of following materials: the calgon of the GABA of the stabilizing agent of the black tea of the white granulated sugar of 20～100g, the milk powder of 10～50g, 1～10g, the emulsifying agent of 0.1～2g, 0.05～2g, the sweetener of 0～0.5g, 0.01～0.2g, the flavoring essence of 0.5～2.0g, 0.1～1.0g, the natrium citricum of 0.05～1.0g, the sodium phosphate trimer of 0～1.0g, nisin and the water of 0.01～0.2g.Through in milk tea beverage, adding the slow factor of pressing of GABA, it is tasty, can alleviate the pressure of live and work after drinking effectively; And milk tea beverage has the characteristic of suitable mass consumption; Be convenient to promote,, in milk tea, merely add GABA though GABA has the character of a lot of excellences; Alleviate the poor effect of the pressure of live and work, need further to improve.
One Chinese patent application CN 101940325A discloses a kind of sports drink that contains GABA; Comprise following component in percentage by weight: 0.005%～0.05% GABA, 10%～30% fruit juice or plant extracts, 3%～8% sugar, 0.005%～0.12% sodium chloride, 0.005%～0.02% potassium chloride, 0.01%～0.09% calcium lactate, 0.01%～0.08% magnesium chloride, 0.01%～0.05% vitamin C, 0.1%～0.3% organic acid, 0～0.1% spices, all the other are pure water.Though contained GABA has the brain of raising vigor in the sports drink that provides, promote the metabolism and recovery cell function, ataraxy of brain tissue; Alleviate stress; Exciting motion potential reduce sports fatigue, but the GABA addition is less relatively in this sports drink; The simple GABA that relies on is alleviated stress and minimizing sports fatigue, and effect is not good enough.
At present, utilize GABA exploitation diet article to become the focus of extensive concern, most of technology mainly concentrates on fields such as utilizing tea beverage, milk-contained drink or fermented wine.Through adding an amount of GABA and multiple amino acid needed by human body is produced the nutrients beverage with specific function,, be the new problem in beverage production field to satisfy people's demand.
Summary of the invention
The invention provides a kind of nutrients beverage that contains GABA, composite through the natural health factor gamma-amino-butyric acid and the compound amino acid of biologically active, the effective pressure of ease people daily life and work.
A kind of nutrients beverage that contains GABA, form by following raw materials by weight percent:
GABA 0.005%～0.05%;
Compound amino acid 0.005%～0.1%;
HFCS 0.1%～6.6%;
Acesulfame potassium 0.01%～0.1%;
Natrium citricum 0.01%～0.1%;
Potassium sorbate 0～0.01%;
Flavoring essence 0.01%～0.1%;
Water surplus.
Above-mentioned raw material all can adopt commercially available product; The raw material of selecting for use all will meet the state food and the drug standards; For example HFCS should meet GB GB/T 20882-2007, and GABA should meet the regulation that Ministry of Health of the People's Republic of China announces 2009 No. 12; Compound amino acid Main Ingredients and Appearance: contain 18 seed amino acids such as glycine, alanine, leucine, isoleucine, valine, cystine, cysteine, methionine, threonine, serine, phenylalanine, tyrosine, tryptophan, proline, hydroxyproline, glutamic acid, asparatate.
Acesulfame potassium has castering action as sweetener to the whole sugariness that contains the nutrients beverage product of GABA.
Flavoring essence belongs to composite food additive, and the whole nutrients beverage product system that contains GABA is played the effect fragrant, that improve mouthfeel of composing.
As preferably; The percentage by weight of described GABA is 0.005%～0.03%; Add the GABA of this percentage by weight at the nutrients beverage that contains GABA; Not influencing under the prerequisite of mouthfeel of nutrients beverage that the present invention contains GABA, make the present invention contain the nutrients beverage performance physiological active functions of GABA through GABA.
As preferably; The percentage by weight of described compound amino acid is 0.005%～0.05%; Add the compound amino acid of this percentage by weight at the nutrients beverage that contains GABA; Do not influencing under the prerequisite of mouthfeel of nutrients beverage that the present invention contains GABA, through compound amino acid enhance immunity power.
The present invention utilizes the physiological active functions of GABA and the effect of compound amino acid enhance immunity power, has collaborative effect between the two, and as preferably, the percentage by weight of described GABA is 0.009%～0.02%; The percentage by weight of described compound amino acid is 0.008%～0.03%; GABA under this percentage by weight and compound amino acid have stronger synergy; Keeping tensions down mood more effectively, thus the pressure of daily life and work alleviated effectively.
As preferably, the described nutrients beverage that contains GABA, form by following raw materials by weight percent:
GABA 0.009%～0.02%;
Compound amino acid 0.008%～0.03%;
HFCS 1%～4%;
Acesulfame potassium 0.018%～0.04%;
Natrium citricum 0.04%～0.08%;
Potassium sorbate 0.0045～0.008%;
Flavoring essence 0.05%～0.1%;
Water surplus.
It is not only tasty that the present invention contains the nutrients beverage of GABA under this proportioning, and mouthfeel is good, and keeping tensions down mood effectively, alleviates the pressure of daily life and work effectively.
The present invention also provides a kind of preparation method who contains the nutrients beverage of GABA, and preparation is simple, and easy to implement, controllability is good.
A kind of preparation method who contains the nutrients beverage of GABA may further comprise the steps:
1) allotment: GABA, compound amino acid, HFCS, acesulfame potassium, natrium citricum, selectivity are added the potassium sorbate and the flavoring essence of (refer to and can add as required or not add) be added to the water; Stir 15min～40min down at 40 ℃～70 ℃; Mix; Continue to stir 5min～30min down at 40 ℃～70 ℃, form the good mixed liquor of allotment;
2) degassing and homogeneous: the mixed liquor that allotment in the step 1) is good outgases earlier, and homogeneous is for use behind the homogeneous again;
3) sterilization and cooling;
4) can and packing obtain containing the nutrients beverage product of GABA.
In the step 1); Preferably stir 20min～30min down, mix, continue to stir 10min～20min down at 50 ℃～60 ℃ at 50 ℃～60 ℃; Make that the raw material dissolving is abundant more and it is more even to mix, thereby improve the dispersiveness of each raw material in the good mixed liquor of allotment.
Step 2) in,, mixes each other more uniformly with each raw material that improves in the mixed liquor through the raw material in the homogeneous micronization mixed liquor; Thereby make mixed liquor more stable; As preferably, processing condition is: homogenizing temperature is 60 ℃～70 ℃, and first class pressure is 15MPa～25MPa; Secondary pressure is 3MPa～7MPa, makes the nutrients beverage product that contains GABA of final preparation have better stability.
Sterilization in the step 3) and cooling belong to those skilled in the art's routine techniques, can pass through existing techniques in realizing.
Can in the step 4) and packing also can adopt prior art.
Compared with prior art, the present invention has following advantage:
The nutrients beverage that the present invention contains GABA is composite through the natural health factor gamma-amino-butyric acid and the compound amino acid of biologically active; Utilize the physiological active functions of GABA and the effect of compound amino acid enhance immunity power; Has collaborative effect between the two; After making that student, old man and the working clan etc. be in moods such as anxiety, fatigue, worry drink the nutrients beverage that the present invention contains GABA; Can the keeping tensions down mood, thus the pressure of daily life and work effectively alleviated.
The present invention contains the nutrients beverage of GABA except that GABA and compound amino acid, also comprises some other composition, and all clear and definite qualification on content; It is not only tasty to make that the present invention contains the nutrients beverage of GABA; Mouthfeel is good, and is very popular, helps the marketization and promotes; Therefore, the present invention's nutrients beverage of containing GABA has the vast market promotion prospect.
The present invention contains the preparation method of the nutrients beverage of GABA, and the existing commercially available equipment that passes through just can be realized suitability for industrialized production, have the preparation simple, easy to implement, be easy to control, high repeatability and other advantages.
The specific embodiment
Embodiment 1
Choose the raw material of following amount: GABA 0.1g, compound amino acid (Jiangmen Quantum High-tech Biology Co., Ltd; The food-grade compound amino acid) 0.1g, HFCS (the first bio tech ltd of the white dragon wound in Shandong, HFCS F42) 19.65g, acesulfame potassium (AK sugar) 0.2g, natrium citricum 0.5g, potassium sorbate 0.05g, flavoring essence (model that the brilliant spring essence and flavoring agent in Hangzhou Co., Ltd produces is 2032 red fuji apple water quality essence) 0.6g and water 1000g;
1) allotment: the GABA of 0.1g, the compound amino acid of 0.1g, the HFCS of 19.65g, the acesulfame potassium (AK sugar) of 0.2g, the natrium citricum of 0.5g, the potassium sorbate of 0.05g and the flavoring essence of 0.6g are joined in the water of 1000g; Stir 25min down at 55 ℃; Make raw material all dissolve; Mix, continue to stir 15min down, form the good mixed liquor of allotment at 55 ℃;
2) degassing and homogeneous: the mixed liquor that allotment in the step 1) is good outgases earlier, homogeneous again, and 65 ℃ of homogenizing temperatures, first class pressure 20MPa, secondary pressure 5MPa, for use behind the homogeneous;
3) sterilization and cooling;
4) can and packing obtain containing the nutrients beverage product of GABA.
Embodiment 2
Choose the raw material of following amount: GABA 0.2g, compound amino acid (Jiangmen Quantum High-tech Biology Co., Ltd; The food-grade compound amino acid) 0.1g, HFCS (the first bio tech ltd of the white dragon wound in Shandong, HFCS F42) 19.65g, acesulfame potassium (AK sugar) 0.2g, natrium citricum 0.5g, potassium sorbate 0.05, flavoring essence (model that the brilliant spring essence and flavoring agent in Hangzhou Co., Ltd produces is 2211 sweet orange water quality essence) 0.6g and water 1000g;
1) allotment: the GABA of 0.2g, the compound amino acid of 0.1g, the HFCS of 19.65g, the acesulfame potassium (AK sugar) of 0.2g, the natrium citricum of 0.5g, the potassium sorbate of 0.05g and the flavoring essence of 0.6g are joined in the water of 1000g; Continue to stir 25min down at 55 ℃; Make raw material all dissolve; Mix, stir 15min down, form the good mixed liquor of allotment at 55 ℃;
2) degassing and homogeneous: the mixed liquor that allotment in the step 1) is good outgases earlier, homogeneous again, and 65 ℃ of homogenizing temperatures, first class pressure 20MPa, secondary pressure 5MPa, for use behind the homogeneous;
3) sterilization and cooling;
4) can and packing obtain containing the nutrients beverage product of GABA.
Embodiment 3
Choose the raw material of following amount: GABA 0.1g, compound amino acid (Jiangmen Quantum High-tech Biology Co., Ltd; The food-grade compound amino acid) 0.3g, HFCS (the first bio tech ltd of the white dragon wound in Shandong, HFCS F42) 19.65g, acesulfame potassium (AK sugar) 0.2g, natrium citricum 0.5g, potassium sorbate 0.05g, flavoring essence (model that the brilliant spring essence and flavoring agent in Hangzhou Co., Ltd produces is 2023 pineapple water quality essence) 0.6g and water 1000g;
1) allotment: the GABA of 0.1g, the compound amino acid of 0.3g, the HFCS of 19.65g, the acesulfame potassium (AK sugar) of 0.2g, the natrium citricum of 0.5g, the potassium sorbate of 0.05g and the flavoring essence of 0.6g are joined in the water of 1000g; Stir 25min down at 55 ℃; Make raw material all dissolve; Mix, continue to stir 15min down, form the good mixed liquor of allotment at 55 ℃;
2) degassing and homogeneous: the mixed liquor that allotment in the step 1) is good outgases earlier, homogeneous again, and 65 ℃ of homogenizing temperatures, first class pressure 20MPa, secondary pressure 5MPa, for use behind the homogeneous;
3) sterilization and cooling;
4) can and packing obtain containing the nutrients beverage product of GABA.
Embodiment 4
Choose the raw material of following amount: GABA 0.1g, compound amino acid (Jiangmen Quantum High-tech Biology Co., Ltd; The food-grade compound amino acid) 0.1g, HFCS (the first bio tech ltd of the white dragon wound in Shandong, HFCS F42) 40g, acesulfame potassium (AK sugar) 0.2g, natrium citricum 0.5g, potassium sorbate 0.05g, flavoring essence (model that the brilliant spring essence and flavoring agent in Hangzhou Co., Ltd produces is 2037 green apple water quality essence) 0.6g and water 1000g;
1) allotment: the GABA of 0.1g, the compound amino acid of 0.1g, the HFCS of 40g, the acesulfame potassium (AK sugar) of 0.2g, the natrium citricum of 0.5g, the potassium sorbate of 0.05g and the flavoring essence of 0.6g are joined in the water of 1000g; Stir 25min down at 55 ℃; Make raw material all dissolve; Mix, continue to stir 15min down, form the good mixed liquor of allotment at 55 ℃;
2) degassing and homogeneous: the mixed liquor that allotment in the step 1) is good outgases earlier, homogeneous again, and 65 ℃ of homogenizing temperatures, first class pressure 20MPa, secondary pressure 5MPa, for use behind the homogeneous;
3) sterilization and cooling;
4) can and packing obtain containing the nutrients beverage product of GABA.
Embodiment 5
Choose the raw material of following amount: GABA 0.1g, compound amino acid (Jiangmen Quantum High-tech Biology Co., Ltd; The food-grade compound amino acid) 0.1g, HFCS (the first bio tech ltd of the white dragon wound in Shandong, HFCS F42) 19.65g, acesulfame potassium (AK sugar) 0.4g, natrium citricum 0.5g, potassium sorbate 0.05g, flavoring essence (model that the brilliant spring essence and flavoring agent in Hangzhou Co., Ltd produces is 2032 red fuji apple water quality essence) 0.6g and water 1000g;
1) allotment: the GABA of 0.1g, the compound amino acid of 0.1g, the HFCS of 19.65g, the acesulfame potassium (AK sugar) of 0.4g, the natrium citricum of 0.5g, the potassium sorbate of 0.05g and the flavoring essence of 0.6g are joined in the water of 1000g; Stir 25min down at 55 ℃; Make raw material all dissolve; Mix, continue to stir 15min down, form the good mixed liquor of allotment at 55 ℃;
2) degassing and homogeneous: the mixed liquor that allotment in the step 1) is good outgases earlier, homogeneous again, and 65 ℃ of homogenizing temperatures, first class pressure 20MPa, secondary pressure 5MPa, for use behind the homogeneous;
3) sterilization and cooling;
4) can and packing obtain containing the nutrients beverage product of GABA.
Embodiment 6
Choose the raw material of following amount: GABA 0.1g, compound amino acid (Jiangmen Quantum High-tech Biology Co., Ltd; The food-grade compound amino acid) 0.1g, HFCS (the first bio tech ltd of the white dragon wound in Shandong, HFCS F42) 19.65g, acesulfame potassium (AK sugar) 0.2g, natrium citricum 0.8g, potassium sorbate 0.05g, flavoring essence (model that the brilliant spring essence and flavoring agent in Hangzhou Co., Ltd produces is 2032 red fuji apple water quality essence) 0.6g and water 1000g;
1) allotment: the GABA of 0.1g, the compound amino acid of 0.1g, the HFCS of 19.65g, the acesulfame potassium (AK sugar) of 0.2g, the natrium citricum of 0.8g, the potassium sorbate of 0.05g and the flavoring essence of 0.6g are joined in the water of 1000g; Stir 20min down at 60 ℃; Make raw material all dissolve; Mix, continue to stir 10min down, form the good mixed liquor of allotment at 60 ℃;
2) degassing and homogeneous: the mixed liquor that allotment in the step 1) is good outgases earlier, homogeneous again, and 65 ℃ of homogenizing temperatures, first class pressure 20MPa, secondary pressure 5MPa, for use behind the homogeneous;
3) sterilization and cooling;
4) can and packing obtain containing the nutrients beverage product of GABA.
Embodiment 7
Choose the raw material of following amount: GABA 0.1g, compound amino acid (Jiangmen Quantum High-tech Biology Co., Ltd; The food-grade compound amino acid) 0.1g, HFCS (the first bio tech ltd of the white dragon wound in Shandong, HFCS F42) 19.65g, acesulfame potassium (AK sugar) 0.2g, natrium citricum 0.5g, potassium sorbate 0.08g, flavoring essence (model that the brilliant spring essence and flavoring agent in Hangzhou Co., Ltd produces is 2032 red fuji apple water quality essence) 0.6g and water 1000g;
1) allotment: the GABA of 0.1g, the compound amino acid of 0.1g, the HFCS of 19.65g, the acesulfame potassium (AK sugar) of 0.2g, the natrium citricum of 0.5g, the potassium sorbate of 0.08g and the flavoring essence of 0.6g are joined in the water of 1000g; Stir 30min down at 50 ℃; Make raw material all dissolve; Mix, continue to stir 20min down, form the good mixed liquor of allotment at 50 ℃;
2) degassing and homogeneous: the mixed liquor that allotment in the step 1) is good outgases earlier, homogeneous again, and 65 ℃ of homogenizing temperatures, first class pressure 20MPa, secondary pressure 5MPa, for use behind the homogeneous;
3) sterilization and cooling;
4) can and packing obtain containing the nutrients beverage product of GABA.
Embodiment 8
Choose the raw material of following amount: GABA 0.1g, compound amino acid (Jiangmen Quantum High-tech Biology Co., Ltd; The food-grade compound amino acid) 0.1g, HFCS (the first bio tech ltd of the white dragon wound in Shandong, HFCS F42) 19.65g, acesulfame potassium (AK sugar) 0.2g, natrium citricum 0.5g, potassium sorbate 0.05g, flavoring essence (model that the brilliant spring essence and flavoring agent in Hangzhou Co., Ltd produces is 2032 red fuji apple water quality essence) 1g and water 1000g;
1) allotment: the GABA of 0.1g, the compound amino acid of 0.1g, the HFCS of 19.65g, the acesulfame potassium (AK sugar) of 0.2g, the natrium citricum of 0.5g, the potassium sorbate of 0.05g and the flavoring essence of 1g are joined in the water of 1000g; Stir 25min down at 55 ℃; Make raw material all dissolve; Mix, continue to stir 15min down, form the good mixed liquor of allotment at 55 ℃;
2) degassing and homogeneous: the mixed liquor that allotment in the step 1) is good outgases earlier, homogeneous again, and 70 ℃ of homogenizing temperatures, first class pressure 16MPa, secondary pressure 4MPa, for use behind the homogeneous;
3) sterilization and cooling;
4) can and packing obtain containing the nutrients beverage product of GABA.
Embodiment 9
Choose the raw material of following amount: GABA 0.1g, compound amino acid (Jiangmen Quantum High-tech Biology Co., Ltd; The food-grade compound amino acid) 0.1g, HFCS (the first bio tech ltd of the white dragon wound in Shandong, HFCS F42) 11g, acesulfame potassium (AK sugar) 0.2g, natrium citricum 0.5g, potassium sorbate 0.05g, flavoring essence (model that the brilliant spring essence and flavoring agent in Hangzhou Co., Ltd produces is 2032 red fuji apple water quality essence) 0.6g and water 1000g;
1) allotment: the GABA of 0.1g, the compound amino acid of 0.1g, the HFCS of 11g, the acesulfame potassium (AK sugar) of 0.2g, the natrium citricum of 0.5g, the potassium sorbate of 0.05g and the flavoring essence of 0.6g are joined in the water of 1000g; Stir 25min down at 55 ℃; Make raw material all dissolve; Mix, continue to stir 15min down, form the good mixed liquor of allotment at 55 ℃;
2) degassing and homogeneous: the mixed liquor that allotment in the step 1) is good outgases earlier, homogeneous again, and 60 ℃ of homogenizing temperatures, first class pressure 24MPa, secondary pressure 6MPa, for use behind the homogeneous;
3) sterilization and cooling;
4) can and packing obtain containing the nutrients beverage product of GABA.
At last, it is also to be noted that what more than enumerate only is specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Test case (test of product antifatigue effect)
One, materials and methods
1) animal divides into groups and administration
A. animal divides into groups
80 of Kunming male adult mouse; Body weight 18g～22g; Mouse is after adapting to 7 days under the experimental situation; Be divided into four groups at random by body weight, promptly blank group (A group), contain GABA nutrients beverage low dose group (B group), contain dose groups (C group) in the nutrients beverage of GABA, contain the nutrients beverage high dose group (D group) of GABA.
B. gastric infusion
According to the requirement of health food functional evaluation, various animals should be established 3 dose groups at least, and wherein 1 dose groups is equivalent to 5 times (mouse) of human body recommended intake (conversion is every kilogram quantities), and maximum dose level is no more than 30 times that human body is recommended.On this basis; This experiment adopt Kunming mouse according to 10 times of human body recommended amounts as lowest dose level; Middle and high dosage is respectively 15 times and 20 times of people's body recommended amounts; Be that basic, normal, high dose groups gives GABA nutrients beverage 1.0,1.5,2.0g/kg.bw respectively, the blank group gives distilled water.Every day, 12:00 irritated stomach, continuous irrigation stomach 30 days, and filling is freely ingested between gastric phase and is drunk water.
2) preparation of sample
Get the nutrients beverage product that contains GABA among the embodiment 1 as sample.
3) experimental technique
A. swimming with a load attached to the body experiment
After last gives mouse given the test agent 30min, place the swimming case to swim the load mouse of 5% body weight sheet lead of root of the tail portion, depth of water 40cm, 25 ± 1.0 ℃ of water temperatures, the record mouse from the swimming beginning to dead time, i.e. mouse swimming with a load attached to the body time;
B. the mensuration of mouse full blood lactic
Behind 30min and the swimming with a load attached to the body 30min, the eye socket blood sampling detects full blood lactic content with the lactate analyzer respectively behind the last filling stomach;
C. the mensuration of Mouse Liver glycogen, muscle glycogen, serum urea nitrogen after moving
After last is irritated stomach 30min, no swimming with a load attached to the body 90min, behind the quiet 60min, eye socket blood sampling separation of serum adopts automatic clinical chemistry analyzer to measure urea nitrogen content.Get mouse liver and quadriceps muscle of thigh rapidly, adopt anthrone colorimetric method for determining hepatic glycogen, muscle glycogen content.
Two, experimental result
1) contains of the influence of the nutrients beverage of GABA to the mouse body weight
The mouse body weight is every other day surveyed in experiment, continuous 30 days, survey its body weight, the GABA nutrients beverage that obtains to the influence of mouse body weight (X ± SD, n=20) as shown in table 1.Compare with control group, body weight quality and body weight quality there was no significant difference (P≤0.5) when finishing explains that absorption GABA nutrients beverage does not make significant difference to the mouse body weight when each dose groups mouse experiment of GABA began.
Table 1
| Group | Initial body weight/g | Finish body weight/g |
| A | 19.37±1.78 | 31.25±2.58 |
| B | 19.14±1.21 | 30.47±3.12 |
| C | 19.78±1.36 | 32.02±2.57 |
| D | 19.65±1.48 | 31.49±2.03 |
2) nutrients beverage that contains GABA exhausts the influence of time to mouse power
The GABA nutrients beverage exhausts the influence (X ± SD) as shown in table 2 of time to mouse power; It is obvious that power exhausts each group difference of time; Compare with the blank group, each dose groups all prolongs to some extent, wherein the best results of high dose group; All have statistical significance (P＜0.05), explain that the nutrients beverage that the present invention contains GABA has the effect that prolongs the mouse swimming time in various degree.
Table 2
| Group (n=6) | Power exhausts time/min |
| A | 21.3±5.32 |
| B | 32.2±9.57 |
| C | 41.5±10.44 |
| D | 57.8±18.75 |
3) contain of the influence of the nutrients beverage of GABA to Mouse Liver glycogen and muscle glycogen
The nutrients beverage that contains GABA to Mouse Liver glycogen and muscle glycogen content to influence the result as shown in table 3; Compare with control group; The high dose group mouse was taken in the GABA nutrients beverage after 30 days; Mouse Liver glycogen storage level extremely significantly increases (P＜0.01), and low dosage and middle dose groups significantly increase (P＜0.05).Muscle glycogen content all is higher than control group behind the mouse movement of each dose groups, and statistical significance (P＜0.05) is arranged.The result shows that nutrients beverage that the present invention contains GABA can increase and is tried the Mouse Liver glycogen and muscle glycogen is laid in that for human body provides more multipotency source material, thereby delay fatigue produces.
Table 3
| Group (n=5) | Hepatic glycogen (mg/g) | Muscle glycogen (mg/g) |
| A | 8.01±1.06 | 1.74±0.21 |
| B | 10.75±1.08 | 2.41±0.31 |
| C | 11.65±1.28 | 2.46±0.54 |
| D | 13.46±2.35 | 2.69±0.78 |
4) contain of the influence of the nutrients beverage of GABA to lactic acid before and after the mouse movement
The nutrients beverage that contains GABA to lactic acid before and after the mouse movement to influence the result as shown in table 4; Compare with control group; The high dose group mouse was taken in the GABA nutrients beverage after 30 days; The blood lactate level extremely significantly is lower than control group (P＜0.01); Middle dose groups and low dose group mouse were taken in the GABA nutrients beverage after 30 days, and the blood lactate level significantly is lower than control group (P＜0.05), explained that the nutrients beverage that contains GABA has the effect of lactic acid accumulation in the better removing body.
Table 4
| Group (n=5) | Lactic acid (mmol/L) before the motion | Motion back lactic acid (mmol/L) |
| A | 16.213±2.598 | 21.609±2.363 |
| B | 16.201±2.314 | 20.965±1.256 |
| C | 16.541±1.874 | 20.254±1.568 |
| D | 16.217±0.156 | 19.123±1.021 |
Three, conclusion
Experimental result shows; The nutrients beverage that the present invention contains GABA can improve mouse movement endurance, raising hepatic glycogen and muscle glycogen storage level, enhancing body is removed the lactic acid ability; According to the requirement of " health food check and assessment technique standard " (2003 editions), the nutrients beverage that judgement the present invention contains GABA has the function of alleviating physical fatigue.
The nutrients beverage that contains GABA of embodiment 2～9 preparations also detects; By contrast; The effect of embodiment 1 is best, and the detection effect of the nutrients beverage that contains GABA of embodiment 2～9 preparations all differs in 10% near embodiment 1.Through randomly drawing 1000 people the nutrients beverage that contains GABA of embodiment 1～9 preparation is tasted evaluation; The nutrients beverage that contains GABA of embodiment 1～9 preparation is all tasty; Mouthfeel is good, and is especially the most popular with the nutrients beverage that contains GABA of embodiment 1 preparation.
The nutrients beverage that the present invention contains GABA meets the regulation of GB/T 10789-2007 beverage general rule and food security method simultaneously.
Claims (8)
1. nutrients beverage that contains GABA, form by following raw materials by weight percent:
GABA 0.005%～0.05%;
Compound amino acid 0.005%～0.1%;
HFCS 0.1%～6.6%;
Acesulfame potassium 0.01%～0.1%;
Natrium citricum 0.01%～0.1%;
Potassium sorbate 0～0.01%;
Flavoring essence 0.01%～0.1%;
Water surplus.
2. the nutrients beverage that contains GABA according to claim 1 is characterized in that, the percentage by weight of described GABA is 0.005%～0.03%.
3. the nutrients beverage that contains GABA according to claim 1 is characterized in that, the percentage by weight of described compound amino acid is 0.005%～0.05%.
4. the nutrients beverage that contains GABA according to claim 1 is characterized in that, the percentage by weight of described GABA is 0.009%～0.02%; The percentage by weight of described compound amino acid is 0.008%～0.03%.
5. the nutrients beverage that contains GABA according to claim 1 is characterized in that, is made up of following raw materials by weight percent:
GABA 0.009%～0.02%;
Compound amino acid 0.008%～0.03%;
HFCS 1%～4%;
Acesulfame potassium 0.018%～0.04%;
Natrium citricum 0.04%～0.08%;
Potassium sorbate 0.0045～0.008%;
Flavoring essence 0.05%～0.1%;
Water surplus.
6. the preparation method who contains the nutrients beverage of GABA according to claim 1 may further comprise the steps:
1) allotment: potassium sorbate and flavoring essence that GABA, compound amino acid, HFCS, acesulfame potassium, natrium citricum, selectivity are added are added to the water; Stir 15min～40min down at 40 ℃～70 ℃; Mix; Continue to stir 5min～30min down at 40 ℃～70 ℃, form the good mixed liquor of allotment;
2) degassing and homogeneous: the mixed liquor that allotment in the step 1) is good outgases earlier, and homogeneous is for use behind the homogeneous again;
3) sterilization and cooling;
4) can and packing obtain containing the nutrients beverage product of GABA.
7. the preparation method who contains the nutrients beverage of GABA according to claim 6 is characterized in that, in the step 1), stirs 20min～30min down at 50 ℃～60 ℃, mixes, and continues to stir 10min～20min down at 50 ℃～60 ℃.
8. the preparation method who contains the nutrients beverage of GABA according to claim 6 is characterized in that step 2) in, processing condition is: homogenizing temperature is 60 ℃～70 ℃, and first class pressure is 15MPa～25MPa, and secondary pressure is 3MPa～7MPa.
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Application publication date: 20120801 |