CN103960171A - Artificial cultivation method for bohadschia argus larvae - Google Patents
Artificial cultivation method for bohadschia argus larvae Download PDFInfo
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- CN103960171A CN103960171A CN201310032664.1A CN201310032664A CN103960171A CN 103960171 A CN103960171 A CN 103960171A CN 201310032664 A CN201310032664 A CN 201310032664A CN 103960171 A CN103960171 A CN 103960171A
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- 241000961078 Bohadschia argus Species 0.000 title claims abstract description 32
- 238000012364 cultivation method Methods 0.000 title abstract 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 21
- 230000007613 environmental effect Effects 0.000 claims abstract description 5
- 241000529895 Stercorarius Species 0.000 claims description 22
- 241000208340 Araliaceae Species 0.000 claims description 18
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 18
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 18
- 235000008434 ginseng Nutrition 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 17
- 238000012258 culturing Methods 0.000 claims description 12
- 230000001418 larval effect Effects 0.000 claims description 11
- 241000593522 Sargassum thunbergii Species 0.000 claims description 8
- 230000016087 ovulation Effects 0.000 claims description 8
- 230000008859 change Effects 0.000 claims description 7
- 230000002710 gonadal effect Effects 0.000 claims description 6
- 230000035800 maturation Effects 0.000 claims description 6
- 241000227752 Chaetoceros Species 0.000 claims description 5
- 241000195493 Cryptophyta Species 0.000 claims description 5
- 241000223252 Rhodotorula Species 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- 230000003203 everyday effect Effects 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 240000002900 Arthrospira platensis Species 0.000 claims description 3
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 3
- 241000237509 Patinopecten sp. Species 0.000 claims description 3
- 241000195474 Sargassum Species 0.000 claims description 3
- 210000001647 gastrula Anatomy 0.000 claims description 3
- 235000012054 meals Nutrition 0.000 claims description 3
- 230000032696 parturition Effects 0.000 claims description 3
- 230000000384 rearing effect Effects 0.000 claims description 3
- 235000020637 scallop Nutrition 0.000 claims description 3
- 229940082787 spirulina Drugs 0.000 claims description 3
- 230000002354 daily effect Effects 0.000 claims description 2
- 230000006698 induction Effects 0.000 claims description 2
- 230000000638 stimulation Effects 0.000 claims description 2
- 235000013601 eggs Nutrition 0.000 abstract description 18
- 239000000203 mixture Substances 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract 1
- 230000037213 diet Effects 0.000 description 4
- 235000005911 diet Nutrition 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 241000965254 Apostichopus japonicus Species 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 241000221377 Auricularia Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000220010 Rhode Species 0.000 description 1
- 229920001617 Vinyon Polymers 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000023011 digestive tract development Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to an artificial cultivation method for bohadschia argus larvae. According to the method, the specification, environmental condition and bait of cultivation of parent bohadschia argus are set, and after growing to specific time, the parent bohadschia argus is subjected to artificial spawning in such a way that the parent bohadschia argus is shielded from light, dried in shade and stimulated by strong water flow. After the parent bohadschia argus releases sperms and eggs to produce fertilized eggs, the survival condition of the fertilized eggs is controlled, optimal fertilized eggs are selected and counted and then enter a larva cultivation stage, larvae are fed with artificially mixed larva feed, and the water quality environment is managed. After the larvae enter a big-ear larva stage, an adhering medium is given, the composition adjustment of bait is started, and water quality management at the later stage of the larvae is carried out. The cultivation is not complete until the larvae develop into young bohadschia argus. Through the adoption of the artificial cultivation method for bohadschia argus larvae, provided by the invention, bohadschia argus is changed from a wild economic variety that can only captured artificially into an economic variety that can be cultivated artificially, and artificial cultivation of bohadschia argus can be started.
Description
Technical field
The present invention relates to a kind of Bohadschia argus Jaeger fingerlings artificial culturing method.
Background technology
Bohadschia argus Jaeger is the economic kind of Tropical Ocean by the present invention's artificial culture newly developed, also there is no the technical data of artificial seed rearing at present.
Artificial seed's breeding method of imitative stichopus japonicus is ripe, propagate the output that can produce 10,000,000,000 yuan artificially, become the Important Economic kind of mariculture, the basis that imitative stichopus japonicus is propagated artificially is exactly that artificial seed cultivates the ripe method of having set up, although the cultivation at seed ginseng, artificial insemination, there are roughly the same step and link in the cultivation aspect of larval culture and young ginseng, but the Developmental Biology of artificial seed's breeding method of Bohadschia argus Jaeger based on Bohadschia argus Jaeger itself, environmental ecology and each stage diet composition feature, the technical method that the concrete artificial seed who invents for the economic kind of this new Tropical Ocean of Bohadschia argus Jaeger cultivates.
Summary of the invention
The object of the invention is to provide a kind of Bohadschia argus Jaeger fingerlings artificial culturing method.
For achieving the above object, the present invention adopts technical scheme to be:
A Bohadschia argus Jaeger fingerlings artificial culturing method, by Bohadschia argus Jaeger parent participation through artificial culture to gonadal maturation; Under the water temperature of 30-31 ℃, by strong stimulation by running water, hasten parturition, induction parent participation ovulation or ejaculation, produce fertilized egg, incubating oosperm is cultivated and is generated the young, until paedomorphosis during to doliolaria, to cultivating in pond, throw in adherance, take salt algae, Chaetoceros, ocean rhodotorula and food calling yeast carries out seed rearing as bait.
With 350 grams to 450 grams Bohadschia argus Jaeger parent participations of specification, in cultivating pond, cultivates to gonadal maturation seed ginseng; In cultivating process, daily ration of feeding is by the 4-6% of parent participation TBW; The environmental condition of cultivating is that water temperature remains on 26 ℃--more than 31 ℃, DO value remain on 5ppm.
Bottom, described cultivation pond is equipped with Sha Heni, and wherein husky and mud is 4:6 in the ratio of quality; Bait forms: 8% 200 order sargassum powder or sargassum thunbergii powder, 90% seabed mud scum, 1% scallop edge powder and 1% 200 order conch meals form.
By cultivating to the parent participation of gonadal maturation, pull out and dry in the shade 40-60 minutes, with the current of 3 meter per second flow velocitys, stimulate after 5-10 minute, seed ginseng is put into water temperature and in the cultivation pond of 30-31 ℃, induce parent participation ovulation or ejaculation.
Incubating oosperm during the initial stage, carries out cloth pond by the young by 0.5/ml to primitive gut later stage or ear shape.
The bait of throwing something and feeding and being formed by salt algae 2ppm, Chaetoceros 4ppm, ocean rhodotorula 2ppm and food calling yeast 1ppm in incubating oosperm to the doliolaria stage; And in the whole larval culture stage, cultivate pool inner water body and change, replacing every day amount is for cultivating the 1/3-1/2 of pond volume.
When paedomorphosis is doliolaria in the water of described cultivation pond, throw in adherance, and make it to be covered with whole cultivation pond, the young is attached on adherance and grows after 11-12 days, usings ooze 5ppm, sargassum thunbergii powder 5ppm, spirulina powder 0.3ppm as bait, until obtain young ginseng.
The present invention has advantages of:
Specification, environmental condition and diet composition that the present invention cultivates by setting parent participation, and grow after the specific time at it, in the mode that lucifuge is dried in the shade and strong current stimulate, carry out artificial induced spawning.After parent participation ovulation, control the life condition of fertilized egg, through fertilized egg preferably and counting, enter the larval culture stage, the artificial young feed coordinating and carry out water quality environment management of throwing something and feeding.The young, enter after large ear larval stage, drop into adherance, and start to adjust bait formation, carry out the water quality management in young later stage, until paedomorphosis is young ginseng.
The artificial culturing method of the Bohadschia argus Jaeger seed that employing the present invention relates to, make Bohadschia argus Jaeger by only can by manually adopt the wild economic kind of catching change into can artificial culture economic kind, can start to propagate artificially.
Embodiment
Embodiment 1
Bohadschia argus Jaeger artificial seed breed:
1) selection of parent participation and cultivation:
The specification that parent participation is cultivated: 350 grams to 450 grams
The temperature that parent participation is cultivated: 26 ℃--31 ℃
The environment that parent participation is cultivated:
Husky mud bottom environment is set in the cement pit of 1. 3.5 meters * 6.5 meters * 1.8 meters, and silt ratio is 4:6;
2. pool inner water is 1.3 meters-1.5 meters deeply, miniflow water, and streamflow makes water in pond be able to whole replacings in 3 days;
3. use rhodes blower inflation, make in breeding water body more than DO value remains on 5ppm;
4. every 5 days, change pond once, at the bottom of the pool wall emptying, pond, with brush, brush the dirt of all kinds of depositions.
The bait that parent participation is cultivated:
1. diet composition (percentage by weight): 8%200 order sargassum powder, 90% seabed mud scum, 1% scallop edge powder and 1%150 order conch meals;
2. bait feeding: throw something and feed every day by the 4-6% of parent participation gross weight in pond.
2) artificial induced spawning of parent participation:
1. parent participation is selected: choose and be above-mentionedly cultured to that 350 grams of parent participation body weight are above, slightly bossed individuality is felt at belly sexual gland position;
2. the mode of hastening parturition: seed ginseng is pulled out and dried in the shade 40-60 minutes from pond, stimulate after 5-10 minute with the current of 3 meter per seconds, seed ginseng is put into spawning pond, the water temperature in spawning pond remains on 30 ° ℃ 31 ℃.
3) ovulation and fertilization:
Under normal circumstances after male ginseng ejaculation soon, female ginseng just starts ovulation; As hero is joined a large amount of ejaculations, should put into storage pond, holding pond in time by just joining and pull out at the hero of ejaculation, ovulation continues 2-4 hour, cultivates the faint inflation in pond, and aeration quantity can make to be evenly distributed in fertilized egg pool water within.
4) fertilized egg preferably and counting
1. micro-Microscopic observation incubating oosperm situation, and counting.Water in hatchery is stirred evenly, with probe tube multidraw in pond, put into the beaker of 1000ml, with 2ml suction pipe, inwardly blow, fertilized egg is evenly distributed in cup, gets 2ml water and observe fertilized egg quantity, according to said method repeatedly sampling, take the mean and be about density of fertilized egg, with density, be multiplied by the quantity that water body is fertilized egg.
2. incubating oosperm can be grown primitive gut later stage or auricularia initial stage after 36 hours, select in time excellent.General with siphonage, fertilized egg in hatching is siphoned in 260 order dredgers, in time the fertilized egg in net is put into tank, after collecting fertilized egg, stirred evenly, according to hatching ovum total amount, by 0.5/ml, carry out cloth pond, faint inflation behind cloth pond, aeration quantity can make fertilized egg be evenly distributed in water.
5) larval culture:
1. in larval culture process, it is complete whether microscopy observation larva digestive tract grows, the bait of should throwing something and feeding in time as complete in its gut development, and diet composition is: salt algae 2ppm, Chaetoceros 4ppm, ocean rhodotorula 2ppm, food calling yeast 1ppm.
2. in larval culture process, change water once every day, and quantity of exchanged water, for cultivating 1/3rd of pond, is progressively increased to 1/2nd.Changing water method is: with 260 order net cages, put into pond siphon water outlet.Siphon is complete will be bonded under larva on net cage sprinkles with clear water.
6) throw in adherance
1. with parcel pendant thing in the middle of 30cm * 30cm vinyon cloth, make it partly to float in water, as the adherance of the young, young adherance should be covered with nursery pond substantially.
2. from larval culture to the, within eight days, starting to observe in time the young and change, is now large ear larval stage.The main variation of observing larva stomach shape and body cavity.When having doliolaria to occur, start to throw in above-mentioned adherance.
3. want timely microscopy larvae development situation, Individual Size, variable density.Throw in adherance second day and start to throw in ooze and sargassum thunbergii powder 4-5ppm, ooze and sargassum thunbergii powder respectively account for 50% by weight.
4. larval culture to the fortnight starts to change throw something and feed ooze 5ppm, sargassum thunbergii powder 5ppm, spirulina powder 0.3ppm.After this visually observe, on pool wall and adherance, visible many little projections, are young ginseng, realize the cultivation of seedling kind.
Then can adjust above-mentioned bait (as adjusted gradually the ratio of sargassum thunbergii powder and ooze) according to the situation of ingesting of young ginseng on adherance and actual needs, more generally at 20-25 days, start down pond according to residual bait situation, with 120 order mesh bags, receive the end.
Adopt above-described embodiment, every ten female ginsengs of fully-developed and two male ginsengs can produce 1,000 ten thousand fertilized eggs, and the quantity that is developed to doliolaria is 3,000,000, and the quantity of growing for young ginseng is 200,000.
Claims (7)
1. a Bohadschia argus Jaeger fingerlings artificial culturing method, is characterized in that: by Bohadschia argus Jaeger parent participation through artificial culture to gonadal maturation; Under the water temperature of 30-31 ℃, by strong stimulation by running water, hasten parturition, induction parent participation ovulation or ejaculation, produce fertilized egg, incubating oosperm is cultivated and is generated the young, until paedomorphosis during to doliolaria, to cultivating in pond, throw in adherance, take salt algae, Chaetoceros, ocean rhodotorula and food calling yeast carries out seed rearing as bait.
2. by Bohadschia argus Jaeger fingerlings artificial culturing method claimed in claim 1, it is characterized in that: with 350 grams to 450 grams Bohadschia argus Jaeger parent participations of specification, in seed ginseng is cultivated pond, cultivate to gonadal maturation; In cultivating process, daily ration of feeding is by the 4-6% of parent participation TBW; The environmental condition of cultivating is that water temperature remains on 26 ℃--more than 31 ℃, DO value remain on 5ppm.
3. by Bohadschia argus Jaeger fingerlings artificial culturing method claimed in claim 2, it is characterized in that: bottom, described cultivation pond is equipped with Sha Heni, wherein husky and mud is 4:6 in the ratio of quality; Bait forms: 8% 200 order sargassum powder or sargassum thunbergii powder, 90% seabed mud scum, 1% scallop edge powder and 1% 200 order conch meals form.
4. by Bohadschia argus Jaeger fingerlings artificial culturing method claimed in claim 1, it is characterized in that: by cultivating to the parent participation of gonadal maturation, pull out and dry in the shade 40-60 minutes, current with 3 meter per second flow velocitys stimulated after 5-10 minute, seed ginseng is put into water temperature and in the cultivation pond of 30-31 ℃, induce parent participation ovulation or ejaculation.
5. by Bohadschia argus Jaeger fingerlings artificial culturing method claimed in claim 1, it is characterized in that: incubating oosperm during the initial stage, carries out cloth pond by the young by 0.5/ml to primitive gut later stage or ear shape.
6. by Bohadschia argus Jaeger fingerlings artificial culturing method claimed in claim 1, it is characterized in that: the bait of throwing something and feeding and being formed by salt algae 2ppm, Chaetoceros 4ppm, ocean rhodotorula 2ppm and food calling yeast 1ppm in incubating oosperm to the doliolaria stage; And in the whole larval culture stage, cultivate pool inner water body and change, replacing every day amount is for cultivating the 1/3-1/2 of pond volume.
7. by Bohadschia argus Jaeger fingerlings artificial culturing method described in claim 1 or 6, it is characterized in that: when paedomorphosis is doliolaria in the water of described cultivation pond, throw in adherance, and make it to be covered with whole cultivation pond, the young is attached on adherance and grows after 11-12 days, using ooze 5ppm, sargassum thunbergii powder 5ppm, spirulina powder 0.3ppm as bait, until obtain young ginseng.
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| CN201310032664.1A CN103960171B (en) | 2013-01-28 | 2013-01-28 | Bohadschia argus Jaeger fingerlings artificial culturing method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106912417A (en) * | 2015-12-25 | 2017-07-04 | 中国科学院海洋研究所 | Bohadschia argus Jaeger industrialized cultivation method |
| CN109804953A (en) * | 2019-04-08 | 2019-05-28 | 中国水产科学研究院黄海水产研究所 | A kind of mating system of purple pseudo- web ginseng artificial seed |
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| CN106912417A (en) * | 2015-12-25 | 2017-07-04 | 中国科学院海洋研究所 | Bohadschia argus Jaeger industrialized cultivation method |
| CN106912417B (en) * | 2015-12-25 | 2020-03-10 | 中国科学院海洋研究所 | Industrial breeding method for Munseng sempervirens |
| CN109804953A (en) * | 2019-04-08 | 2019-05-28 | 中国水产科学研究院黄海水产研究所 | A kind of mating system of purple pseudo- web ginseng artificial seed |
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