CN103946378B - 葡糖淀粉酶变体和编码它们的多核苷酸 - Google Patents

葡糖淀粉酶变体和编码它们的多核苷酸 Download PDF

Info

Publication number
CN103946378B
CN103946378B CN201280050093.8A CN201280050093A CN103946378B CN 103946378 B CN103946378 B CN 103946378B CN 201280050093 A CN201280050093 A CN 201280050093A CN 103946378 B CN103946378 B CN 103946378B
Authority
CN
China
Prior art keywords
glucoamylase
another aspect
variant
seq
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201280050093.8A
Other languages
English (en)
Chinese (zh)
Other versions
CN103946378A (zh
Inventor
E.P.弗里斯
L.德马里亚
J.文德
T.A.波尔森
A.斯文德森
S.达尼尔森
R.T.伦哈德
H.弗里斯-马德森
L.K.斯科夫
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of CN103946378A publication Critical patent/CN103946378A/zh
Application granted granted Critical
Publication of CN103946378B publication Critical patent/CN103946378B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242Fungal source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/20Aspartic acid; Asparagine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/20Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/002Preparation of hydrocarbons or halogenated hydrocarbons cyclic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44Polycarboxylic acids
    • C12P7/46Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44Polycarboxylic acids
    • C12P7/48Tricarboxylic acids, e.g. citric acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/54Acetic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56Lactic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001Alpha-amylase (3.2.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201280050093.8A 2011-10-11 2012-10-11 葡糖淀粉酶变体和编码它们的多核苷酸 Active CN103946378B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161545628P 2011-10-11 2011-10-11
US61/545,628 2011-10-11
PCT/EP2012/070127 WO2013053801A1 (en) 2011-10-11 2012-10-11 Glucoamylase variants and polynucleotides encoding same

Publications (2)

Publication Number Publication Date
CN103946378A CN103946378A (zh) 2014-07-23
CN103946378B true CN103946378B (zh) 2018-04-24

Family

ID=47049149

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280050093.8A Active CN103946378B (zh) 2011-10-11 2012-10-11 葡糖淀粉酶变体和编码它们的多核苷酸

Country Status (10)

Country Link
US (8) US9353362B2 (enExample)
EP (1) EP2766476B1 (enExample)
CN (1) CN103946378B (enExample)
CA (1) CA2853000C (enExample)
DK (1) DK2766476T3 (enExample)
EA (1) EA201490649A1 (enExample)
ES (1) ES2638669T3 (enExample)
IN (1) IN2014CN03468A (enExample)
MX (1) MX351762B (enExample)
WO (1) WO2013053801A1 (enExample)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5568307B2 (ja) * 2006-10-10 2014-08-06 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン 修飾された性質を有するグルコアミラーゼ変異体
WO2014135647A1 (en) * 2013-03-07 2014-09-12 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
CA2915065C (en) 2013-06-24 2022-11-01 Novozymes A/S Processes for recovering oil from fermentation product processes and processes for producing fermentation products
HUE040545T2 (hu) 2013-07-17 2019-03-28 Novozymes As Pullulanáz kimérák és az azokat kódoló polinukleotidok
EP3712274A1 (en) 2013-09-11 2020-09-23 Novozymes A/S Processes for producing fermentation products
HUE056192T2 (hu) 2014-01-22 2022-01-28 Novozymes As Pullulanázvariánsok és azokat kódoló polinukleotidok
WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
CN104480127B (zh) * 2014-12-14 2017-04-05 长春中医药大学 超嗜热糖苷酶突变体及其在人参皂苷ck制备中的应用
WO2016196202A1 (en) 2015-05-29 2016-12-08 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
CN108350442A (zh) 2015-06-18 2018-07-31 诺维信公司 具有海藻糖酶活性的多肽及其在产生发酵产物的方法中的用途
US20180208916A1 (en) 2015-07-23 2018-07-26 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
US10494685B2 (en) * 2015-10-14 2019-12-03 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US10597645B2 (en) 2015-12-22 2020-03-24 Novozymes A/S Process of extracting oil from thin stillage
CN110072997A (zh) 2016-11-23 2019-07-30 诺维信公司 具有蛋白酶活性的多肽和编码其的多核苷酸
US10889836B2 (en) 2016-11-23 2021-01-12 Novozymes A/S Yeast for ethanol production
US20230002749A1 (en) * 2017-03-07 2023-01-05 Danisco Us Inc. Thermostable glucoamylase and methods of use, thereof
WO2018222990A1 (en) 2017-06-02 2018-12-06 Novozymes A/S Improved yeast for ethanol production
EP3645555B1 (en) 2017-06-28 2021-12-08 Novozymes A/S Polypeptides having trehalase activity and polynucleotides encoding same
CN111094562A (zh) 2017-08-08 2020-05-01 诺维信公司 具有海藻糖酶活性的多肽及其在产生发酵产物的方法中的用途
BR112020004476A2 (pt) 2017-09-15 2020-09-08 Novozymes A/S processo para produção de um produto de fermentação, e, uso de uma combinação de enzimas
CN111148830A (zh) 2017-10-04 2020-05-12 诺维信公司 具有蛋白酶活性的多肽和编码其的多核苷酸
MX2020003981A (es) 2017-10-23 2020-08-03 Novozymes As Procesos para reducir acido lactico en un sistema de fermentacion de biocombustible.
CN107828855B (zh) * 2017-10-31 2020-08-21 南京欣迪生物药业工程有限责任公司 一种精浆中性α-葡糖苷酶检测试剂盒及其应用
EP3720955B1 (en) 2017-12-08 2023-06-14 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
CA3081096A1 (en) * 2017-12-08 2019-06-13 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
CA3089135A1 (en) 2018-01-29 2019-08-01 Novozymes A/S Microorganisms with improved nitrogen utilization for ethanol production
BR112020016677A2 (pt) 2018-02-15 2020-12-15 Novozymes A/S Levedura melhorada para a produção de etanol
MX2020010526A (es) 2018-04-09 2021-01-08 Novozymes As Polipéptidos que tienen actividad alfa-amilasa ypolinucleótidos que los codifican.
CN112166197A (zh) 2018-05-31 2021-01-01 诺维信公司 用于增强酵母生长和生产力的方法
WO2020014407A1 (en) 2018-07-11 2020-01-16 Novozymes A/S Processes for producing fermentation products
EP3827088A1 (en) * 2018-07-25 2021-06-02 Novozymes A/S Enzyme-expressing yeast for ethanol production
BR112021006692A2 (pt) 2018-10-08 2021-08-10 Novozymes A/S levedura expressando enzimas para produção de etanol
EP3918060A1 (en) 2019-01-31 2021-12-08 Novozymes A/S Polypeptides having xylanase activity and use thereof for improving the nutritional quality of animal feed
US20220186266A1 (en) 2019-04-02 2022-06-16 Novozymes A/S Process For Producing A Fermentation Product
CA3143381A1 (en) 2019-07-26 2021-02-04 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
BR112022002203A2 (pt) 2019-08-05 2022-09-06 Novozymes As Misturas de enzimas e processos para produção de um ingrediente alimentar com alto teor de proteína a partir de um subproduto de vinhaça completa
CA3143527A1 (en) 2019-08-06 2021-02-11 Novozymes A/S Fusion proteins for improved enzyme expression
AU2020402990A1 (en) 2019-12-10 2022-06-09 Novozymes A/S Microorganism for improved pentose fermentation
CA3158832A1 (en) 2019-12-16 2021-06-24 Chee-Leong Soong Processes for producing fermentation products
MX2022009515A (es) 2020-02-10 2022-09-02 Novozymes As Variantes de alfa-amilasa y polinucleotidos que las codifican.
BR112023008361A2 (pt) * 2020-11-02 2023-12-12 Novozymes As Variantes de glucoamilase e polinucleotídeos codificando as mesmas
CN112359027B (zh) * 2021-01-13 2021-04-13 凯莱英生命科学技术(天津)有限公司 细胞色素p450酶突变体及其应用
CN115386506A (zh) * 2021-04-16 2022-11-25 中国科学院亚热带农业生态研究所 促进畜禽粪便和秸秆快速堆肥的微生物复合菌剂及其生产生物有机肥的方法
EP4352241A1 (en) 2021-06-07 2024-04-17 Novozymes A/S Engineered microorganism for improved ethanol fermentation
CA3225830A1 (en) * 2021-07-12 2023-01-19 Joshua W. Heyen Strains of saccharomyces cerevisiae that exhibit an increased ability to ferment oligosaccharides into ethanol without supplemental glucoamylase and methods of making and using the sam
CN113881654B (zh) * 2021-09-08 2023-06-09 暨南大学 一种对胃蛋白酶抗性提高的α-淀粉酶
WO2024137250A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
CN120693401A (zh) 2022-12-19 2025-09-23 诺维信公司 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
WO2024137704A2 (en) 2022-12-19 2024-06-27 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
CN120380140A (zh) 2022-12-19 2025-07-25 诺维信公司 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1(ce1)多肽和编码其的多核苷酸
WO2024258820A2 (en) 2023-06-13 2024-12-19 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000034452A1 (en) * 1998-12-07 2000-06-15 Novozymes A/S Glucoamylases with n-terminal extensions
WO2011127802A1 (en) * 2010-04-14 2011-10-20 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US108941A (en) * 1870-11-01 Improvement in portable forges
DK135983D0 (da) 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
EP0383779B2 (en) 1987-09-04 2000-05-31 Novo Nordisk A/S PROCESS FOR THE PRODUCTION OF PROTEIN PRODUCTS IN $i(ASPERGILLUS) AND PROMOTERS FOR USE IN $i(ASPERGILLUS)
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DK81293D0 (da) 1993-07-06 1993-07-06 Novo Nordisk As Enzym
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
AU694954B2 (en) 1994-06-03 1998-08-06 Novo Nordisk A/S Purified myceliophthora laccases and nucleic acids encoding same
EP1559776A3 (en) 1994-06-30 2006-01-11 Novozymes Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system and promoters and terminators for use therein
BR9607013B1 (pt) 1995-02-03 2011-03-09 processo de construção de uma variante de uma alfa-amilase de bacillus original derivada de uma cepa de b. licheniformis, de b. amyloliquefaciens, de b. stearothermophilus ou de um bacillus sp. alcalofìlico.
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
BR9708887B1 (pt) 1996-04-30 2014-10-29 Novozymes As "variante de uma alfa-amilase, uso da mesma, construção de dna, vetor de expressão recombinante, célula de bactéria ou fungo, aditivo e composição detergente".
CA2305191C (en) 1997-10-13 2011-09-27 Novo Nordisk A/S .alpha.-amylase mutants
DK1032654T3 (da) 1997-11-26 2009-05-11 Novozymes As Thermostabil glucoamylase
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
CA2759907A1 (en) 1998-02-27 1999-09-02 Novozymes A/S Maltogenic alpha-amylase variants
CA2331340A1 (en) 1998-07-15 2000-01-27 Novozymes A/S Glucoamylase variants
US6352851B1 (en) * 1998-07-15 2002-03-05 Novozymes A/S Glucoamylase variants
CN1197965C (zh) 1998-10-26 2005-04-20 诺维信公司 在丝状真菌细胞内构建和筛选目的dna文库
CN1526820B (zh) 1999-03-22 2010-05-26 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
WO2000060059A2 (en) 1999-03-30 2000-10-12 NovozymesA/S Alpha-amylase variants
CA2416967C (en) 2000-08-01 2014-02-18 Novozymes A/S Alpha-amylase mutants with altered properties
DE60222914T2 (de) 2001-07-27 2008-07-24 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden
US20050010037A1 (en) 2001-12-07 2005-01-13 Wenping Wu Polypeptides having protease activity and nucleic acids encoding same
DE04718914T1 (de) 2003-03-10 2006-02-23 Novozymes A/S Verfahren zur herstellung von alkohol
ES2425351T3 (es) 2003-06-25 2013-10-14 Novozymes A/S Polipéptidos que tienen actividad de alfa-amilasa y polinucleótidos que codifican los mismos
EP1648996B1 (en) 2003-06-25 2012-03-14 Novozymes A/S Enzymes for starch processing
DK1682655T3 (da) 2003-10-30 2010-01-11 Novozymes As Carbohydratbindende moduler fra en ny familie
DK2365068T3 (en) 2004-12-22 2017-05-15 Novozymes As ENZYMER FOR PROCESSING STARCH
DE602007003225D1 (de) 2006-06-15 2009-12-24 Novozymes North America Inc Verfahren zur herstellung eines stärkehydrolysats
EP2260105B1 (en) 2008-02-29 2016-08-17 The Trustees Of The University Of Pennsylvania Production and use of plant degrading materials
US8132578B2 (en) * 2008-07-17 2012-03-13 Elc Management Llc Sifter dish insert for cosmetic package
MX2012006548A (es) 2009-12-11 2012-07-10 Novozymes North America Inc Variantes de proteasa.
EP2521771A1 (en) 2010-01-04 2012-11-14 Novozymes A/S Stabilization of alpha-amylases towards calcium depletion and acidic ph
US9617527B2 (en) * 2010-04-14 2017-04-11 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
US9816112B2 (en) * 2010-12-22 2017-11-14 Novozymes A/S Processes for producing fermentation products
ES2935920T3 (es) * 2012-03-30 2023-03-13 Novozymes North America Inc Procesos de elaboración de productos de fermentación

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000034452A1 (en) * 1998-12-07 2000-06-15 Novozymes A/S Glucoamylases with n-terminal extensions
WO2011127802A1 (en) * 2010-04-14 2011-10-20 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
嗜高温酶的研究及应用;吴军林等;《广州食品工业科技》;20031231;第19卷(第1期);60-62、84 *

Also Published As

Publication number Publication date
CA2853000A1 (en) 2013-04-18
US20170342393A1 (en) 2017-11-30
EA201490649A1 (ru) 2014-07-30
CN103946378A (zh) 2014-07-23
US10865399B2 (en) 2020-12-15
US20250236859A1 (en) 2025-07-24
MX351762B (es) 2017-10-26
MX2014004292A (es) 2014-07-24
IN2014CN03468A (enExample) 2015-07-03
EP2766476A1 (en) 2014-08-20
US9765318B2 (en) 2017-09-19
US20240084277A1 (en) 2024-03-14
US10113158B2 (en) 2018-10-30
EP2766476B1 (en) 2017-05-31
DK2766476T3 (en) 2017-08-28
WO2013053801A1 (en) 2013-04-18
US20210062172A1 (en) 2021-03-04
US20160230157A1 (en) 2016-08-11
US20140255997A1 (en) 2014-09-11
US20170073655A1 (en) 2017-03-16
US9540624B2 (en) 2017-01-10
ES2638669T3 (es) 2017-10-23
US20190002856A1 (en) 2019-01-03
US9353362B2 (en) 2016-05-31
US11840713B2 (en) 2023-12-12
CA2853000C (en) 2022-03-15

Similar Documents

Publication Publication Date Title
CN103946378B (zh) 葡糖淀粉酶变体和编码它们的多核苷酸
CN105164253B (zh) 葡糖淀粉酶变体和编码它们的多核苷酸
CN103930543B (zh) 葡糖淀粉酶变体和编码它们的多核苷酸
CN105960457B (zh) 普鲁兰酶变体以及编码它们的多核苷酸
WO2014177541A2 (en) Glucoamylase variants and polynucleotides encoding same
US9695406B2 (en) Glucoamylase variants and polynucleotides encoding same

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant