CN103918543B - A kind of On Transportaion of Live and the method storing Kappa Trentepohlia algae - Google Patents
A kind of On Transportaion of Live and the method storing Kappa Trentepohlia algae Download PDFInfo
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- CN103918543B CN103918543B CN201310010473.5A CN201310010473A CN103918543B CN 103918543 B CN103918543 B CN 103918543B CN 201310010473 A CN201310010473 A CN 201310010473A CN 103918543 B CN103918543 B CN 103918543B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention belongs to field of marine biotechnology, refer specifically to On Transportaion of Live and the method for storage of Kappa Trentepohlia algae [mainly including kappaphycus alvarezi (Kappaphycus alvarezii) and different branch Kappa algae (Kappaphycus striatum)].Main contents of the present invention include: the results of healthy Kappa Trentepohlia algae process and preservation condition selects.The method is simple, the keep-alive phase is long, it is adaptable to solve the long-distance transport of Kappa Trentepohlia algae, and at typhoon period, it is ensured that algae culturing family gathers in the crops seedling in advance, and reaches safe storage seedling, evades the purpose that seedling is lost.The present invention takes the technical measures from water, moisturizing, temperature control and dark.Suitably reduce the vitality of Kappa Trentepohlia Sargassum from water purpose, extend and store and haulage time；Moisturizing can prevent Sargassum to be dried, and maintains its analytic metabolism ability together with temperature control；And dark can avoid Sargassum generation photic damage, reach to extend storage and haulage time purpose.
Description
Technical field
The invention belongs to field of marine biotechnology, relate to Kappa Trentepohlia algae and [mainly include kappaphycus alvarezi (Kappaphycus
Alvarezii) and different branch Kappa algae (Kappaphycus striatum)] On Transportaion of Live and storing technology.
Background technology
Kappaphycus alvarezi (Kappaphycus alvarezii) and different branch Kappa algae (Kappaphycus
Striatum) it is the primary raw material producing κ type carrageenan in the world, and κ type carrageenan has extensive use in the food and medicine industry such as ham sausage, ice cream, fruit jelly, milk product.
20 century 70s start, Southeast Asia tropic countries such as Philippine, Indonesia, Malaysia, Vietnam etc., and part Central and South American countries (Brazil, Mexico etc.) and portions of Africa country (Tanzania, Kenya etc.) successively start both Sargassums are carried out artificial cultivation.Both Sargassum raw materials only seldom come from the wild Sargassum in the Tropical Ocean natural habitats such as coral reef at present, and both Sargassum raw materials of industrial use are derived from artificial cultivation mostly.The artificial Sargassum such as large-scale planting kappaphycus alvarezi and different branch Kappa algae, produces for carrageenan in the world and provides basic material, and provide support to industry developments such as relevant food processing further.
Fish evil, the algae that grows nonparasitically upon another plant, canker, water temperature restriction and typhoon etc. usually govern Kappa Trentepohlia algae culturing.Wherein, fish evil, grow nonparasitically upon another plant algae and canker often results in Kappa Trentepohlia algae extensive death, cause the in short supply of follow-up cultivation healthy seedling.Therefore.Artificial cultivation must be restarted from other area long-distance transport cultivation seedling.And in long-distance transport, how controlling temperature, humidity etc., the activity keeping Kappa segasso sea dish is most important, the most still lacks the technical measures effectively preserving Ottelia acuminata (Gagnep.) Dandy activity.
Being suitable for the growth temperature of Kappa Trentepohlia algae typically at 20-30 DEG C, only from sea temperature angle analysis, little in the perennial marine site meeting above-mentioned seaweed growth of China, only Sanya, Hainan and Lingshui can meet Kappa Trentepohlia algal grown.Therefore, current China produces the Kappa algae raw material of carrageenan, more than 90% dependence on import.For expanding Kappa Trentepohlia algal culture scale, the autonomous shortage problem solving China's Kappa Trentepohlia algae raw material, China north and south sea warm season diversity can be utilized in theory, when Summer in Northern Part marine site temperature conditions is suitable, from south, Kappa Trentepohlia algae seedling is transported to the north cultivate, when marine site, the north in winter temperature conditions is not in good time, Kappa Trentepohlia algae seedling transported to south from the north and cultivates.In Kappa Trentepohlia algae north and south interaction breeding process, the most effectively On Transportaion of Live Kappa algae is the most extremely important.
It addition, Kappa Trentepohlia algae is generally also the multiple region of typhoon and Lan Zi fish migration sea area in the culture zone of China.Before typhoon or blue sub-fish arrive, results Kappa algae safe keep-alive in time stores seedling, not only can evade typhoon and fish evil loss, it is possible to provide sufficient cultivation seedling, it is to avoid the seedling that typhoon or fish evil cause is in short supply.Therefore, exploitation Kappa segasso sea dish keep-alive storing technology can be to solve this problem to provide support to ensure.
For a long time; the most never Kappa Trentepohlia Sargassum surviving transportation method of rigorous and scientific, torrid areas seedling is after long-distance transport, often due to temperature is too high causes death of rotting; or causing Ottelia acuminata (Gagnep.) Dandy to damage to plants caused by sudden drop in temperature even freeze injury because cooling is improper, result affects the vigor of Sargassum.How same humidity controls not will be apparent from.Even if (gathering in the crops conservation before meeting with typhoon) when areal is not required to transport, can put into this kind of Ottelia acuminata (Gagnep.) Dandy to fill in advance in the storage pool of sea water and preserve, owing to Sargassum self can secret out of halo peroxide, cause the decline of algae Seedling vigor to even result in seedling to occur rotting, some seems healthy seedling and is linked in the sea after typhoon, does not also escape final dead destiny of rotting.
In sum, in Kappa alvarezii seaweed produces, it is badly in need of a kind of bottom line of exploitation and reduces this Sargassum vitality method, for On Transportaion of Live or the storage of Kappa algae seedling, ensure the sustainable development of this algal cultivation industry.
Summary of the invention
For solving the problems referred to above, the purpose of the present invention is for providing a kind of On Transportaion of Live and the method storing Kappa Trentepohlia algae, and the long-term keep-alive for Kappa Trentepohlia algae seedling stores and long-distance transport.
The technical scheme is that, a kind of Kappa Trentepohlia algae On Transportaion of Live and the technical method of storage, take from water, moisturizing, temperature control and the technical measures of dark storage.
Wherein, described from water refer to by Kappa Trentepohlia algae from sea gather in the crops after, drip fall surface moisture.
Wherein, described moisturizing refers to that the Kappa Trentepohlia algae drained puts into Sheng storage container, and puts into the water suction moisturizing material soaking sea water in advance.
Preferably, described water suction moisturizing material includes: one or several in Caulis et Folium Lini, Boehmeria, cotton and chemical fibre.
Preferably, for ensureing moistening effect, putting in container by described Kappa Trentepohlia algae, described container includes small basket, bucket, sack and can ensure the transport vehicle of humidity, space, room or pond；During moisturizing, needing to control to adjust humidity, the relative humidity controlled is about 85 ± 10%.
Preferably, within the temperature range of described temperature control refers to be placed in 17.0-20.0 DEG C from water moisturizing material.
Preferably, described dark is stored as complete lucifuge or luminance index in 50 μm ol
photons m−2 s−1Within.
It is a feature of the present invention that and take, from series technique measures such as water, moisturizing, temperature control and dark, to keep the vigor of Kappa Trentepohlia Sargassum dish Seedling.Wherein, suitably reduce Kappa Trentepohlia algae vitality from water purpose, extend and store and haulage time；Moisturizing can prevent Kappa Trentepohlia algae to be dried, and maintains its analytic metabolism ability together with temperature control；And dark can avoid photic damage to occur, reach equally to extend to store and haulage time purpose.
Keep-alive of the present invention store Kappa Trentepohlia Sargassum technical method particularly as follows:
(1) by healthy Kappa alvarezii seaweed seedling, being at sea put in the string bag after results, drain rapidly the moisture on surface, the degree drained does not drips with water with the string bag and is as the criterion；Kappa alvarezii seaweed seedling after draining is loaded in Sheng storage container and stores.In this operating process, the Ottelia acuminata (Gagnep.) Dandy seedling after should as far as possible avoiding results is placed under light direct irradiation, reduces unnecessary photic damage；
(2) under the conditions of the container containing the fresh and alive Ottelia acuminata (Gagnep.) Dandy of storage being put in the different temperature of gradient, humidity and lighting gradients, cultivation two weeks of taking out after the difference storage time and plunge into the commercial sea, observe its upgrowth situation.In the observation period, using the specific growth rate of Kappa algae as the index evaluating treatment effect.Pass through serial experiment, it is determined that keep-alive store Kappa Trentepohlia algae optimum condition be: temperature 17-20 DEG C, relative humidity about 85 ± 10%, dark or the most weak indoor light condition；
(3) when high-volume stores and transports, Kappa algae is planted vegetables and processes from water according to step (1) and put in Sheng storage container, simultaneously at the moisturizing absorbent material containing in the middle of storage container bottom, surrounding and Ottelia acuminata (Gagnep.) Dandy or top placement sea water immersion is crossed, these materials include but not limited to Caulis et Folium Lini, Boehmeria, cotton and the various chemical fibre preferable clothes of water absorption etc., are in certain humidity with Sargassum in ensureing container；The immersion degree of moisturizing material is with the most not a large amount of moisture content that leach as standard；
(4) during aforesaid operations, it should be noted that the most firmly extrude Sargassum, it is to avoid Sargassum fractures and other damage occurs.
Pass through serial experiment, it is determined that keep-alive store Kappa Trentepohlia algae optimum condition be temperature 17-20 DEG C, relative humidity about 85 ± 10%, dark or the most weak indoor light condition.The Sargassum transported under the conditions of above-mentioned and store, at least can ensure its vitality in 5 days, can continue on for marine cultivation as seedling.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that these embodiments are merely to illustrate the present invention rather than limit the scope of the present invention.In addition, it is to be understood that after having read present disclosure, the present invention can be made various changes or modifications by those skilled in the art, these equivalent form of values fall within the scope that the application institute appended claims limits equally.
Embodiment 1
(1) taking healthy kappaphycus alvarezi seedling 30 strain, drain the moisture on surface, weigh every strain 30 g, and wherein 5 strains are as comparison, directly puts back to and marine keeps hanging cultivation.Store it addition, other 25 strain kappaphycus alvarezi seedling to be loaded in bubble chamber respectively (bubble chamber size about 50 cm × 80 cm) from water；
Before kappaphycus alvarezi seedling (hereinafter referred to as Ottelia acuminata (Gagnep.) Dandy) puts into bubble chamber, bottom bubble chamber, first place the absorbent towels that sea water soaks completely, then one layer of Ottelia acuminata (Gagnep.) Dandy Seedling of lay is on wet towel, one layer of wet towel is overlayed on this layer of Ottelia acuminata (Gagnep.) Dandy seedling, continue remaining Ottelia acuminata (Gagnep.) Dandy seedling is put into bubble chamber, finally repaving one layer of wet towel on seedling, the humidity condition of towel is as the criterion to drip the most outward.
During aforesaid operations, it is to avoid firmly extruding Ottelia acuminata (Gagnep.) Dandy causes the unnecessary equivalent damage that fractures to occur.
(2) bubble chamber is placed on temperature 14.0 ± 1.5 DEG C and in illumination is dark incubator.Store different time (my god) after, take 5 strain kappaphycus alvarezi labellings successively the most at random and keep hanging in marine cultivation, and measure kappaphycus alvarezi in the marine weight (as shown in table 1) keeping hanging the last fortnight；
(3) in experimentation, through repeatedly measuring the relative humidity in bubble chamber, result display relative humidity changes in the range of 70%-100%, this variation and bubble chamber degree of closure and put into soak absorbent material quantity relevant, in generally closing preferable case, relative humidity is at 85%-100%, and during case-opening goods-taking dish, the relative humidity around Ottelia acuminata (Gagnep.) Dandy drops to about 50%.
Table 1. 14.0 ± 1.5 DEG C, dark under water condition of storage, the kappaphycus alvarezi seedling of preservation different time keeps hanging in marine fresh weight change
Embodiment 2
(1) sample according to method in embodiment 1, and according to embodiment 1 being placed kappaphycus alvarezi seedling and laying towel wet from sea water；
(2) bubble chamber that will be equipped with Ottelia acuminata (Gagnep.) Dandy is placed under temperature 17.0 ± 1.5 DEG C and dark condition, after storing different time, takes 5 strain kappaphycus alvarezi the most at random and again keeps hanging in marine, measures kappaphycus alvarezi in the marine weight (as shown in table 2) keeping hanging the last fortnight.
Table 2. 17.0 ± 1.5 DEG C, dark under water condition of storage, the kappaphycus alvarezi seedling of preservation different time keeps hanging in marine fresh weight change
Embodiment 3
(1) sample according to method in embodiment 1, and according to embodiment 1 being placed kappaphycus alvarezi seedling and laying towel wet from sea water；
(2) bubble chamber is placed in temperature 20.0 ± 1.5 DEG C and dark culturing case, after storing different number of days, takes 5 strain kappaphycus alvarezi the most at random and again keep hanging in marine, measure kappaphycus alvarezi in the marine weight (as shown in table 3) keeping hanging the last fortnight；
Table 3. 20.0 ± 1.5 DEG C, dark under water condition of storage, the kappaphycus alvarezi seedling of preservation different time keeps hanging in marine fresh weight change
Embodiment 4
(1) sample according to method in embodiment 1, and according to embodiment 1 being placed kappaphycus alvarezi seedling and laying the towel soaked；
(2) bubble chamber is placed in temperature 23.0 ± 1.5 DEG C and dark culturing case, after storing different number of days, takes 5 strain kappaphycus alvarezi the most at random and again keep hanging in marine, measure kappaphycus alvarezi in the marine weight (as shown in table 4) keeping hanging the last fortnight.
Table 4. 23.0 ± 1.5 DEG C, dark under water condition of storage, the kappaphycus alvarezi seedling of preservation different time keeps hanging in marine fresh weight change
Embodiment 5
Sample according to method in embodiment 1, and place Kappa algae seedling according to embodiment 1, bubble chamber and Sargassum are placed in 20 ± 1.5 DEG C of temperature environments, or Sargassum is not carried out moisturizing process, or it is placed under high light, after preserving 5 days, stored Kappa algae or rot or kill, even if some moistening Sargassum keeps hanging in marine cultivation, the most do not observe that growth sign occurs in Sargassum, all rot to later stage almost all of Sargassum.It addition, in 23 DEG C and temperatures above environment, even if in dark, moisturizing and preserving 5 days under water environment, Ottelia acuminata (Gagnep.) Dandy keeps hanging after marine, does not also escape the destiny that almost all of Ottelia acuminata (Gagnep.) Dandy all rots.
Can draw from above-mentioned case study on implementation data, Kappa algae is in temperature 17-20 DEG C, dark and under the conditions of water stores (humidity is maintained at 85%-100%), and within 5 day time, Ottelia acuminata (Gagnep.) Dandy remains in that vitality.
It is more than the description to the embodiment of the present invention, by the described above to the disclosed embodiments, makes professional and technical personnel in the field be capable of or use the present invention.Multiple amendment to these embodiments will be apparent from for those skilled in the art, and generic principles defined herein can realize without departing from the spirit or scope of the present invention in other embodiments.Therefore, the present invention is not intended to be limited to the embodiments shown herein, and is to fit to the widest scope consistent with principles disclosed herein and features of novelty.
Claims (5)
1. a Kappa Trentepohlia algae On Transportaion of Live and the technical method of storage, it is characterised in that
Take from water, moisturizing, temperature control and the technical measures of dark storage；
Described moisturizing refers to that the Kappa Trentepohlia algae drained puts into Sheng storage container, and puts into and soak in advance
Cross the water suction moisturizing material of sea water；
Within the temperature range of described temperature control refers to be placed in 17.0-20.0 DEG C from water moisturizing material.
2. according to the Kappa Trentepohlia algae On Transportaion of Live described in claim 1 and the technical side of storage
Method, it is characterised in that described from water refer to by Kappa Trentepohlia algae from sea gather in the crops after, drip fall table
The moisture in face.
3. according to the Kappa Trentepohlia algae On Transportaion of Live described in claim 1 and the technology of storage
Method, it is characterised in that described water suction moisturizing material includes: Caulis et Folium Lini, Boehmeria, cotton and
One or several in chemical fibre.
4. according to the Kappa Trentepohlia algae On Transportaion of Live described in claim 1 and the technology of storage
Method, it is characterised in that for ensureing moistening effect, described Kappa Trentepohlia algae is put into container
In, described container includes small basket, bucket, sack and can ensure the transport vehicle of humidity, room
Between or pond；During moisturizing, needing to control to adjust humidity, the relative humidity controlled exists
85 ± 10%.
5. according to the Kappa Trentepohlia algae On Transportaion of Live described in claim 1 and the technology of storage
Method, it is characterised in that described dark is stored as complete lucifuge or luminance index in 50 μm ol
photons m-2s-1Within.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1694084A1 (en) * | 1988-12-29 | 1991-11-30 | Институт физиологии растений АН СССР | Method for preserving fragments of thallus angelica with carpospores |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1694084A1 (en) * | 1988-12-29 | 1991-11-30 | Институт физиологии растений АН СССР | Method for preserving fragments of thallus angelica with carpospores |
Non-Patent Citations (3)
| Title |
|---|
| 海带苗的出室、运输和暂养;翁学勇;《科学养鱼》;20101231(第5期);第36-37页 * |
| 螺旋藻藻种保藏方法;李士强等;《齐鲁渔业》;20011231;第18卷(第2期);第37页 * |
| 鹅掌菜的长途运输和人工养殖试验;吴超元等;《生物学通报》;19601231(第3期);第104-106页 * |
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