CN103891485A - Non-tissue-culture rapid propagation method of dragon fruit seedlings - Google Patents
Non-tissue-culture rapid propagation method of dragon fruit seedlings Download PDFInfo
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- CN103891485A CN103891485A CN201210575184.5A CN201210575184A CN103891485A CN 103891485 A CN103891485 A CN 103891485A CN 201210575184 A CN201210575184 A CN 201210575184A CN 103891485 A CN103891485 A CN 103891485A
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Abstract
The invention relates to the technical field of vegetative propagation, in particular to a non-tissue-culture rapid propagation method of dragon fruit seedlings. The method comprises the step that a production seedling substrate is composed of straw crushing objects, animal waste, red mud, river sand and a solid nutrient solution, wherein the solid nutrient solution is composed of a high multiple water absorption water-retaining agent, ABT1 rooting powder and clear water. The production seedling substrate is loose, good in breathability, excellent in water retention capacity and high in fertilizer efficiency, the embryogenic callus of the separated stem of a dragon fruit is quickly formed, a root is formed fast, and the purposes of rooting and sprouting as soon as possible are achieved. After the production seedling substrate is arranged in a cup bag, a 5-10 cm dragon fruit separated stem is inserted in the substrate, then multiple nutrient cup bags are arrayed to form a seedling bed, disinfectant is sprayed, a small arch shed is erected for management, and finished dragon seedlings can be cultivated quickly. Through the non-tissue-culture rapid propagation method, dragon fruit seeding industrialization, large-scale and intensive production is better facilitated.
Description
Technical field
The present invention relates to a kind of plant asexual multiplication technology field, be specifically related to the non-tissue culture and rapid propagation method of a kind of dragon fruit seedling.
Technical background
Dragon fruit is cactaceous triangular prism platymiscium.Originate in Central America torrid zone desert area such as Brazil, Mexico, belong to typical tropical plants.Dragon fruit is nutritious, function is unique, and it contains the rare vegetalitas albumin of general plant and anthocyan and abundant vitamin and water-soluble meals fiber.Its appearance meat scale is like the outer squama of flood dragon, and when huge flower bursts forth, drifting fragrance overflows, and makes people have the sense of luckiness, so also claim " lucky fruit ".Dragon fruit occupies upper reaches in mankind's edible plant fruit, and accumulate the meaning numerous glorious ideals of the mankind such as lucky, healthy, rich and honour, long-lived, no matter people pay a formal visit to kith and kin or visit patient, and the indispensable dragon fruit of the fruit basket that carries conveniently central authorities is indicating and manyly well wishing.So, development plantation dragon fruit, prospect is limitless.
Dragon fruit fruit and seed is many, once obtains a large amount of seedlings with planting seed, but the seedling genetic variation and genetic differentiation of gained and variation are large.In production, conventionally adopt cuttage, grafting or tissue culture propagating.Wherein, the traditional cottage propagation method of dragon fruit is: select the substantial stipes of growth, be cut into the segment of long 15 centimetres, then insert husky bed, after taking root, transplant seedbed.Traditional cottage propagation law technology is extensive, and rooting rate is slow, at the bottom of planting percent.Grafting breeding method is: select to do stock without damage by disease and insect, robust growth, the full dragon fruit seedling of stem meat, then stems of dragon fruits is used to cutter section, scion is inserted to stock, aim at and form layer, tie with cotton thread fixing, wound engage after can continue to cultivate into face by shift-in temporary seedbed.Grafting breeding method seedling speed is fast, but operation is loaded down with trivial details, and the stock fertile material of application is many, cause finished product dragon fruit seedling production cost high.Knit and cultivate breeding numerous and detoxification soon, improve seedling quality and breeding number, but required infrastructure equipment has high input, technical matters specialty and complicated, formation finished product its cycle of seedling is longer.
Summary of the invention
Object of the present invention is exactly overcome the deficiency of above-mentioned technology and the brand-new non-tissue culture and rapid propagation method of kind dragon fruit seedling is provided.Specifically comprise: produce with seedling medium by powder of straw mince, animal wastes, red soil, river sand, solid-state shape nutrient solution form.Wherein, solid-state shape nutrient solution is to be prepared from by water-loss reducer, ABT1 root-inducing powder and the clear water of height water suction multiple; With seedling medium, not only loose, good permeability, water retention property are good in the production of composition, and fertilizer efficiency is high, and the callus of dragon fruit Cut stem forms soon, root formation is also fast, and reaches the object of the seedling of taking root as early as possible.Pack the dragon fruit Cut stem of plugging again 5～10cm after cup bag by producing into seedling medium, then nutrition cup bag is put into the ridge-up bed of growing seedlings, spray disinfectant, the little shed management of frame, quickly breeding goes out dragon fruit finished product seedling.
The present invention is achieved through the following technical solutions:
1, the non-tissue culture and rapid propagation method of dragon fruit seedling, comprises the steps: in technical scheme
(1) select fast numerous place: select to ventilate on the sunny side, around pollution-free source, row irrigation easily plot as fast numerous place;
(2) prepare seedling medium: matrix by powder of straw mince 50～55%, animal wastes 15%, red soil 20～25%, river sand 10% form, several raw materials mix after fermentation becomes seedling medium for subsequent use, is finally mixed into as production seedling medium by the ratio that seedling medium for subsequent use and solid-state shape nutrient solution are 100: 20～30 in mass ratio;
(3) seedling medium pack: production is sub-packed in nutrition cup bag for subsequent use with seedling medium;
(4) fast numerous material is selected and is processed: select without damage by disease and insect, robust growth, the full dragon fruit plant of stem meat is as fast numerous material, and dragon fruit plant is cut into the Cut stem of 5～10cm;
(5) fast numerous material dress cup: Cut stem base portion is inserted into nutrition cup bag, inserts 2～3cm in matrix;
(6) set up the ridge-up bed of growing seedlings: it is the ridge-up bed of growing seedlings that 100～120cm, length conform to fast numerous place that nutrition cup bag is put into wide, then spray the carbendazim thimerosal of 300～500 times toward whole ridge-up bed, finally give little shed on whole ridge-up bed frame, and hide transparent plastic foil;
(7) management main points: at interval of 3～5 days, the plastic foil of little shed is raised ventilative 1～2 hour, hidden again transparent plastic foil after then spraying again the carbendazim thimerosal of 300～500 times;
(8) become transplantation of seedlings: when the Cut stem in nutrition cup bag occurs after callus, plastic foil can be removed, spray water twice sooner or later to every day the nutrition cup bag seedling in ridge-up bed, after Cut stem is taken root, water spray number of times is kept to once a day, and root grows to 3～4cm and just can transplant.
2, the non-tissue culture and rapid propagation method of dragon fruit seedling, the solid-state shape nutrient solution preparation method described in technical scheme is as follows:
(1) raw material composition: water suction multiple is take root pulvis, clear water of water-loss reducer, ABT1～No. 6 of 200～300 times;
(2) preparation method: first will add again the nutrient solution that clear water is made into 10～200,000 times after No. 6 root-inducing powder dilution agent of ABT1～, then by water-loss reducer, nutrient solution is absorbed and becomes solid-state shape nutrient solution.
3, the non-tissue culture and rapid propagation method of dragon fruit seedling, in technical scheme, fast numerous time of dragon fruit seedling is annual optimum spring and summer.
Tool of the present invention has the following advantages:
1, quick-breeding method technical measures uniqueness proposed by the invention, easily implements.
2, by implementing the present invention, breed dragon fruit seedling material used few, only need the dragon fruit Cut stem of 5～10cm can be multiplied into a young plant.
3, by implementing the present invention, seedling medium proportioning science, reasonable for the production of composition, agricultural crop straw increases seedling medium organic crumb and permeability, water-loss reducer has absorbed 200～300 times of nutrient solutions that are prepared from by root-growing agent and has formed solid-state shape nutrient solution, solid-state shape nutrient solution not only discharges slowly moisture and keeps matrix moistening in matrix, and the nutrient solution that discharges out also realized and lured for a long time root, in matrix, admix 15% animal wastes, effectively raise the fertilizer efficiency of matrix; With seedling medium, not only loose, good permeability, water retention property are good in the production of composition, and fertilizer efficiency is high, and the callus of dragon fruit Cut stem forms soon, root formation is also fast, and reaches the object of the seedling of taking root as early as possible.
4, by implementing the present invention, produce with seedling medium raw material wide material sources used, application cost is low.
5, by implementing the present invention, the dragon fruit seedling seedling speed of breeding is fast, survival rate is high.
6,, by implementing the present invention, be more conducive to that dragon fruit is seedling industrialized, scale, intensive production.
Embodiment
Below in conjunction with embodiment, method of the present invention is further illustrated.
The non-tissue culture and rapid propagation method of a kind of dragon fruit seedling, embodiment is as follows:
Embodiment 1:
1, the fast numerous Best Times of dragon fruit seedling carried out in two seasons of annual spring and summer.
2, select fast numerous place: select to ventilate on the sunny side, around pollution-free source, row irrigation easily plot as fast numerous place.
3, prepare solid-state shape nutrient solution: first will after the dilution of ABT1 root-inducing powder, add again the nutrient solution that clear water is made into 200,000 times, then be that 200～300 times of water-loss reducers absorb nutrient solution to become solid-state shape nutrient solution by water suction multiple.
4, prepare seedling medium: matrix is made up of 55% waste edible fungus gurry, 15% animal wastes, 20% red soil, 10% river sand, several raw materials mix after fermentation becomes seedling medium for subsequent use, is finally mixed into as production seedling medium by the ratio that seedling medium for subsequent use and solid-state shape nutrient solution are 100: 25 in mass ratio.
5, seedling medium pack: production is sub-packed in nutrition cup bag for subsequent use with seedling medium.
6, fast numerous material is selected and is processed: select without damage by disease and insect, robust growth, the full dragon fruit plant of stem meat is as fast numerous material, and dragon fruit plant is cut into the Cut stem of 5～8cm.
7, fast numerous material dress cup: Cut stem base portion is inserted into nutrition cup bag, inserts 2～3cm in matrix.
8, set up the ridge-up bed of growing seedlings: it is the ridge-up bed of growing seedlings that 100～120cm, length conform to fast numerous place that nutrition cup bag is put into wide, then spray the carbendazim thimerosal of 400 times toward whole ridge-up bed, finally give little shed on whole ridge-up bed frame, and hide transparent plastic foil.
9, management main points: at interval of 3 days, the plastic foil of little shed is raised ventilative 1 hour, hidden again transparent plastic foil after then spraying again the carbendazim thimerosal of 400 times.
10, become transplantation of seedlings: when the Cut stem in nutrition cup bag occurs after callus, plastic foil can be removed, sooner or later give every day the nutrition cup bag seedling spray in ridge-up bed 2 water, after Cut stem is taken root, water spray number of times is kept to once a day, and root grows to 3～4cm and just can transplant.
Embodiment 2:
1, the fast numerous Best Times of dragon fruit seedling carried out in two seasons of annual spring and summer.
2, select fast numerous place: select to ventilate on the sunny side, around pollution-free source, row irrigation easily plot as fast numerous place.
3, prepare solid-state shape nutrient solution: first will after the dilution of ABT2 root-inducing powder, add again the nutrient solution that clear water is made into 150,000 times, then be that 200～300 times of water-loss reducers absorb nutrient solution to become solid-state shape nutrient solution by water suction multiple.
4, prepare seedling medium: matrix is made up of 50% peanut shell, 15% animal wastes, 25% red soil, 10% river sand, several raw materials mix after fermentation becomes seedling medium for subsequent use, is finally mixed into as production seedling medium by the ratio that seedling medium for subsequent use and solid-state shape nutrient solution are 100: 30 in mass ratio.
5, seedling medium pack: production is sub-packed in nutrition cup bag for subsequent use with seedling medium.
6, fast numerous material is selected and is processed: select without damage by disease and insect, robust growth, the full dragon fruit plant of stem meat is as fast numerous material, and dragon fruit plant is cut into the Cut stem of 5～6cm.
7, fast numerous material dress cup: Cut stem base portion is inserted into nutrition cup bag, inserts 2～3cm in matrix.
8, set up the ridge-up bed of growing seedlings: it is the ridge-up bed of growing seedlings that 100～120cm, length conform to fast numerous place that nutrition cup bag is put into wide, then spray the carbendazim thimerosal of 500 times toward whole ridge-up bed, finally give little shed on whole ridge-up bed frame, and hide transparent plastic foil.
9, management main points: at interval of 4 days, the plastic foil of little shed is raised ventilative 2 hours, hidden again transparent plastic foil after then spraying again the carbendazim thimerosal of 500 times.
10, become transplantation of seedlings: when the Cut stem in nutrition cup bag occurs after callus, plastic foil can be removed, spray water twice sooner or later to every day the nutrition cup bag seedling in ridge-up bed, after Cut stem is taken root, water spray number of times is kept to once a day, and root grows to 3～4cm and just can transplant.
Claims (3)
1. the non-tissue culture and rapid propagation method of dragon fruit seedling, its feature comprises the steps:
(1) select fast numerous place: select to ventilate on the sunny side, around pollution-free source, row irrigation easily plot as fast numerous place;
(2) prepare seedling medium: matrix is made up of agricultural crop straw 50～55%, animal wastes 15%, red soil 20～25%, river sand 10%, after several raw materials are mixed, become seedling medium for subsequent use through everfermentation, be finally mixed into as production seedling medium by the ratio that seedling medium for subsequent use and solid-state shape nutrient solution are 100: 20～30 in mass ratio;
(3) seedling medium pack: production is sub-packed in nutrition cup bag for subsequent use with seedling medium;
(4) fast numerous material is selected and is processed: select without damage by disease and insect, robust growth, the full dragon fruit plant of stem meat is as fast numerous material, and dragon fruit plant is cut into the Cut stem of 5～10cm;
(5) fast numerous material dress cup: Cut stem base portion is inserted into nutrition cup bag, inserts 2～3cm in matrix;
(6) set up the ridge-up bed of growing seedlings: it is the ridge-up bed of growing seedlings that 100～120cm, length conform to fast numerous place that nutrition cup bag is put into wide, then spray the carbendazim thimerosal of 300～500 times toward whole ridge-up bed, finally give little shed on whole ridge-up bed frame, and hide transparent plastic foil;
(7) management main points: at interval of 3～5 days, the plastic foil of little shed is raised ventilative 1～2 hour, then hidden back again transparent plastic foil again after the carbendazim thimerosal of 300～500 times of whole ridge-up bed sprinklings;
(8) become transplantation of seedlings: when the Cut stem in nutrition cup bag occurs after callus, plastic foil can be removed, spray water twice sooner or later to every day the nutrition cup bag seedling in ridge-up bed, after Cut stem is taken root, water spray number of times is kept to once a day, and root grows to 3～4cm and just can transplant.
2. according to the non-tissue culture and rapid propagation method of a kind of dragon fruit seedling described in claim 1, it is characterized in that the solid-state shape nutrient solution preparation method described in step (2) is as follows:
(1) raw material composition: water suction multiple is take root pulvis, clear water of water-loss reducer, ABT1～No. 6 of 200～300 times;
(2) preparation method: first will add again the nutrient solution that clear water is made into 10～200,000 times after No. 6 root-inducing powder dilution agent of ABT1～, then by water-loss reducer, nutrient solution is absorbed and becomes solid-state shape nutrient solution.
3. according to the non-tissue culture and rapid propagation method of a kind of dragon fruit seedling described in claim 1, it is characterized in that fast numerous time of dragon fruit seedling is annual optimum spring and summer.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104303783A (en) * | 2014-10-22 | 2015-01-28 | 重庆市地龙农业有限公司 | Cultivating method of dragon fruits |
| CN105557322A (en) * | 2016-01-25 | 2016-05-11 | 成都美农达农业开发有限责任公司 | Greenhouse planting method for high-sweetness dragon fruits |
| CN106211958A (en) * | 2016-07-06 | 2016-12-14 | 丘以明 | A kind of method of Hylocereus undatus cutting propagation |
| CN106518464A (en) * | 2016-12-07 | 2017-03-22 | 重庆维得鲜农业发展有限公司 | Culture medium of flammulina velutipes and preparation method of culture medium |
| CN106518574A (en) * | 2016-10-25 | 2017-03-22 | 潘克稳 | Planting method of hyloceseus |
| CN106508364A (en) * | 2016-10-25 | 2017-03-22 | 潘克稳 | Golden dragon fruit planting method |
| CN106508360A (en) * | 2016-10-25 | 2017-03-22 | 潘克稳 | Planting method for purple-flesh pitaya |
| CN106576762A (en) * | 2016-11-25 | 2017-04-26 | 兴业县恩民农业科技有限公司 | Planting method of selenium-enriched pitaya |
| CN106577109A (en) * | 2016-11-25 | 2017-04-26 | 兴业县恩民农业科技有限公司 | Seedling culturing method of pitaya |
| CN108812027A (en) * | 2018-07-05 | 2018-11-16 | 井冈山农业科技园管理委员会 | A kind of well ridge honey shaddock standardization method for culturing seedlings |
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- 2012-12-27 CN CN201210575184.5A patent/CN103891485A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104303783A (en) * | 2014-10-22 | 2015-01-28 | 重庆市地龙农业有限公司 | Cultivating method of dragon fruits |
| CN105557322A (en) * | 2016-01-25 | 2016-05-11 | 成都美农达农业开发有限责任公司 | Greenhouse planting method for high-sweetness dragon fruits |
| CN105557322B (en) * | 2016-01-25 | 2018-03-02 | 成都美农达农业开发有限责任公司 | A kind of greenhouse gardening method of high sugariness dragon fruit |
| CN106211958A (en) * | 2016-07-06 | 2016-12-14 | 丘以明 | A kind of method of Hylocereus undatus cutting propagation |
| CN106518574A (en) * | 2016-10-25 | 2017-03-22 | 潘克稳 | Planting method of hyloceseus |
| CN106508364A (en) * | 2016-10-25 | 2017-03-22 | 潘克稳 | Golden dragon fruit planting method |
| CN106508360A (en) * | 2016-10-25 | 2017-03-22 | 潘克稳 | Planting method for purple-flesh pitaya |
| CN106576762A (en) * | 2016-11-25 | 2017-04-26 | 兴业县恩民农业科技有限公司 | Planting method of selenium-enriched pitaya |
| CN106577109A (en) * | 2016-11-25 | 2017-04-26 | 兴业县恩民农业科技有限公司 | Seedling culturing method of pitaya |
| CN106518464A (en) * | 2016-12-07 | 2017-03-22 | 重庆维得鲜农业发展有限公司 | Culture medium of flammulina velutipes and preparation method of culture medium |
| CN108812027A (en) * | 2018-07-05 | 2018-11-16 | 井冈山农业科技园管理委员会 | A kind of well ridge honey shaddock standardization method for culturing seedlings |
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Application publication date: 20140702 |