CN103890180A - 在丝状真菌宿主细胞中产生多种重组多肽的方法 - Google Patents

在丝状真菌宿主细胞中产生多种重组多肽的方法 Download PDF

Info

Publication number
CN103890180A
CN103890180A CN201280052277.8A CN201280052277A CN103890180A CN 103890180 A CN103890180 A CN 103890180A CN 201280052277 A CN201280052277 A CN 201280052277A CN 103890180 A CN103890180 A CN 103890180A
Authority
CN
China
Prior art keywords
cellobiohydrolase
homologous
seq
gene
polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201280052277.8A
Other languages
English (en)
Chinese (zh)
Inventor
D·亚韦
金绮明
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Priority to CN201810953356.5A priority Critical patent/CN109136114A/zh
Publication of CN103890180A publication Critical patent/CN103890180A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0115—Oligoxyloglucan reducing-end-specific cellobiohydrolase (3.2.1.150)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201280052277.8A 2011-08-24 2012-08-23 在丝状真菌宿主细胞中产生多种重组多肽的方法 Pending CN103890180A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810953356.5A CN109136114A (zh) 2011-08-24 2012-08-23 在丝状真菌宿主细胞中产生多种重组多肽的方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161526809P 2011-08-24 2011-08-24
US61/526,809 2011-08-24
PCT/US2012/052143 WO2013028912A2 (en) 2011-08-24 2012-08-23 Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201810953356.5A Division CN109136114A (zh) 2011-08-24 2012-08-23 在丝状真菌宿主细胞中产生多种重组多肽的方法

Publications (1)

Publication Number Publication Date
CN103890180A true CN103890180A (zh) 2014-06-25

Family

ID=47358264

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201280052277.8A Pending CN103890180A (zh) 2011-08-24 2012-08-23 在丝状真菌宿主细胞中产生多种重组多肽的方法
CN201810953356.5A Pending CN109136114A (zh) 2011-08-24 2012-08-23 在丝状真菌宿主细胞中产生多种重组多肽的方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201810953356.5A Pending CN109136114A (zh) 2011-08-24 2012-08-23 在丝状真菌宿主细胞中产生多种重组多肽的方法

Country Status (7)

Country Link
US (3) US9493790B2 (enExample)
EP (1) EP2748322A2 (enExample)
CN (2) CN103890180A (enExample)
AU (1) AU2012298799B2 (enExample)
BR (1) BR112014004190A2 (enExample)
IN (1) IN2014CN02136A (enExample)
WO (1) WO2013028912A2 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107429255A (zh) * 2015-03-09 2017-12-01 诺维信公司 将多种表达构建体引入真核细胞的方法
CN110724643A (zh) * 2019-11-18 2020-01-24 河南省农业科学院植物保护研究所 一株具有防病促生功能的橘灰青霉及其菌剂和应用
CN113302305A (zh) * 2018-12-12 2021-08-24 诺维信公司 提高丝状真菌细胞在多肽的产生方面的生产力的方法

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103748226A (zh) * 2011-08-24 2014-04-23 诺维信股份有限公司 获得丝状真菌宿主细胞的阳性转化体的方法
AU2012298799B2 (en) * 2011-08-24 2018-02-01 Novozymes, Inc. Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
EP2800809B1 (en) 2012-01-05 2018-03-07 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
DK3019602T3 (en) 2013-07-10 2018-11-12 Glykos Finland Oy MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
US20150147774A1 (en) * 2013-11-22 2015-05-28 National University Of Singapore Expression construct for yeast and a method of using the construct
WO2015187935A1 (en) 2014-06-06 2015-12-10 Novozymes A/S Enzyme compositions and uses thereof
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
ES2896153T3 (es) 2014-09-23 2022-02-24 Novozymes As Procedimientos para producir etanol y organismos de fermentación
EP4410974A3 (en) 2016-03-02 2025-04-02 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
WO2018093752A1 (en) * 2016-11-15 2018-05-24 Danisco Us Inc. Filamentous fungi with improved protein production
US20190345522A1 (en) 2016-12-06 2019-11-14 Novozymes A/S Improved Processes For Production Of Ethanol From Xylose-Containing Cellulosic Substrates Using Engineered Yeast Strains
US11091753B2 (en) 2017-05-31 2021-08-17 Novozymes A/S Xylose fermenting yeast strains and processes thereof for ethanol production
WO2018222990A1 (en) 2017-06-02 2018-12-06 Novozymes A/S Improved yeast for ethanol production
CN109988714B (zh) * 2017-12-29 2021-12-28 青岛蔚蓝生物集团有限公司 一种里氏木霉及其应用
MX2020007914A (es) 2018-01-29 2020-10-28 Novozymes As Microorganismos con uso de nitrogeno mejorado para produccion de etanol.
MX2021000893A (es) 2018-07-25 2021-03-31 Novozymes As Levadura que expresa enzimas para la producción de etanol.
MX2021003955A (es) 2018-10-08 2021-05-27 Novozymes As Levadura que expresa enzimas para la produccion de etanol.
AU2020321930A1 (en) 2019-07-26 2022-01-06 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
WO2021025872A1 (en) 2019-08-06 2021-02-11 Novozymes A/S Fusion proteins for improved enzyme expression
CA3158982A1 (en) 2019-12-10 2021-06-17 Monica TASSONE Microorganism for improved pentose fermentation
CA3191025A1 (en) 2020-09-04 2022-03-10 Roberto Nobuyuki Maeda Improved fermenting organism for ethanol production
BR112023025624A2 (pt) 2021-06-07 2024-02-27 Novozymes As Célula de levedura recombinante, célula hospedeira recombinante, composição, cocultura, métodos de produção de um derivado de uma célula hospedeira recombinante e de produção de um produto de fermentação, e, uso de uma célula hospedeira recombinante
CN114561303B (zh) * 2022-02-14 2023-11-17 大连理工大学 一株分泌高性能纤维素酶的里氏木霉工程菌株及其应用
CN115651933A (zh) * 2022-06-06 2023-01-31 福州市皮肤病防治院 高效精准替换红色毛癣菌基因的方法
CN120344672A (zh) 2022-09-23 2025-07-18 诺维信公司 用于乙醇生产的改进发酵生物
WO2025088155A1 (en) 2023-10-25 2025-05-01 Novozymes A/S Improved fermenting organism for ethanol production
CN117757643B (zh) * 2024-01-12 2025-06-13 华东理工大学 一种分泌表达人溶菌酶的里氏木霉工程菌及其构建方法和应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101535340A (zh) * 2006-05-19 2009-09-16 格利科菲公司 促红细胞生成素组合物
WO2010039889A2 (en) * 2008-09-30 2010-04-08 Novozymes, Inc. Methods for using positively and negatively selectable genes in a filamentous fungal cell
CN101970651A (zh) * 2007-05-31 2011-02-09 诺维信股份有限公司 用于降解纤维素材料的组合物

Family Cites Families (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI864473L (fi) * 1985-11-20 1987-05-21 Panlabs Inc Sammansatta yttryckskassetter foer fungaltransformering.
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5989870A (en) 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
US5648263A (en) 1988-03-24 1997-07-15 Novo Nordisk A/S Methods for reducing the harshness of a cotton-containing fabric
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
CA2093424C (en) 1990-10-05 2000-05-02 Kathleen A. Clarkson Methods for treating cotton-containing fabrics with cellulase
EP0655890B1 (en) * 1992-07-31 2005-01-26 AB Enzymes GmbH Recombinant cells, dna constructs, vectors and methods for expression phytate degrading enzymes in desired ratios
EP1479765B1 (en) 1993-03-10 2008-10-22 Novozymes A/S Enzymes with xylanase activity from aspergillus aculeatus
JP3649338B2 (ja) 1994-06-03 2005-05-18 ノボザイムス バイオテック，インコーポレイティド 精製されたマイセリオフトラ・ラッカーゼ及びそれをコードする核酸
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
US20030044956A1 (en) 1995-08-23 2003-03-06 Short Jay M. Enzymes having carboxymethyl cellulase activity and methods of use thereof
EP1605050A3 (en) 1996-01-19 2006-03-22 Novozymes Biotech, Inc. Morphological mutants of filamentous fungi
EP0966521A1 (en) * 1996-09-13 1999-12-29 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide
US6451063B1 (en) 1996-09-25 2002-09-17 Genencor International, Inc. Cellulase for use in industrial processes
US6017870A (en) 1996-10-09 2000-01-25 Genencor International, Inc. Purified cellulase and method of producing
US5811381A (en) 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US6265204B1 (en) * 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
US6558937B1 (en) 1997-07-31 2003-05-06 Dsm N.V. Cellulose degrading enzymes of aspergillus
US5871550A (en) 1997-08-26 1999-02-16 Genencor International, Inc. Mutant Thermonospora spp. cellulase
EP1034280B1 (en) 1997-11-19 2006-05-31 Genencor International, Inc. Cellulase produced by actinomycetes and method of producing same
AU1726299A (en) 1997-12-16 1999-07-05 Genencor International, Inc. Novel egiii-like enzymes, dna encoding such enzymes and methods for producing such enzymes
ES2143408B1 (es) * 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
EP1078080A1 (en) * 1998-05-15 2001-02-28 Novo Nordisk Biotech, Inc. Methods for producing polypeptides in filamentous fungal mutant cells
CN1940067A (zh) 1999-03-22 2007-04-04 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
JP2004527261A (ja) 2001-05-18 2004-09-09 ノボザイムス アクティーゼルスカブ セロビアーゼ活性を有するポリペプチド及びそれをコードするポリヌクレオチド
US6982159B2 (en) 2001-09-21 2006-01-03 Genencor International, Inc. Trichoderma β-glucosidase
US7045331B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. EGVII endoglucanase and nucleic acids encoding the same
US7005289B2 (en) 2001-12-18 2006-02-28 Genencor International, Inc. BGL5 β-glucosidase and nucleic acids encoding the same
US7049125B2 (en) 2001-12-18 2006-05-23 Genencor International, Inc. EGVIII endoglucanase and nucleic acids encoding the same
US7045332B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. BGL4 β-glucosidase and nucleic acids encoding the same
US7056721B2 (en) 2001-12-18 2006-06-06 Genencor International, Inc. EGVI endoglucanase and nucleic acids encoding the same
JP4756861B2 (ja) 2002-08-16 2011-08-24 ジェネンコー・インターナショナル・インク 新規なハイプロクレアジェコリーナｃｂｈ１セルラーゼ
WO2004043980A2 (en) 2002-11-07 2004-05-27 Genencor International, Inc. Bgl6 beta-glucosidase and nucleic acids encoding the same
US7407788B2 (en) 2002-11-21 2008-08-05 Danisco A/S, Genencor Division BGL7 beta-glucosidase and nucleic acids encoding the same
EP2311942A1 (en) 2003-03-21 2011-04-20 Genecor International, Inc. Novel cbh1 homologs and variant cbh1 cellulases
JP5427342B2 (ja) 2003-04-01 2014-02-26 ジェネンコー・インターナショナル・インク 変異体フミコーラ・グリセアｃｂｈ１．１
DK2228440T3 (da) 2003-05-02 2013-01-02 Novozymes Inc Varianter af beta-glucosidaser
DK1862539T3 (da) 2003-05-29 2012-05-14 Danisco Us Inc Nye trichodermagener
EP1682656B1 (en) 2003-10-28 2013-09-18 Novozymes Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
ES2468366T3 (es) 2004-01-30 2014-06-16 Novozymes Inc. Polip�ptidos con actividad de mejora celulol�tica y polinucle�tidos que los codifican
ES2389442T3 (es) 2004-02-06 2012-10-26 Novozymes Inc. Polipéptidos con actividad de aumento celulolítica y polinucleótidos que los codifican
EP1715736A4 (en) * 2004-02-12 2008-09-03 Novozymes Inc POLYPEPTIDES HAVING XYLANASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME
US8097445B2 (en) 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
BRPI0509212A (pt) 2004-03-25 2007-08-28 Genencor Int proteìna de fusão de celulase e construto de fusão de celulase heterólogo codificando a mesma
CN101094917B (zh) 2004-12-30 2012-04-25 金克克国际有限公司 新型变体红褐肉座菌cbh2纤维素酶
CA2593246C (en) 2005-01-06 2016-06-21 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
AR053066A1 (es) 2005-04-26 2007-04-18 Novozymes As Arabinofuranosidasas
BRPI0609906A8 (pt) 2005-04-29 2017-11-21 Ab Enzymes Oy proteína de fusão de celulase, seu processo de produção e composição detergente contendo a mesma, vetor de expressão, célula hospedeira, preparação de enzima, processos para desbotamento e para bioacabamento e métodos de tratamento de fibra celulósica contendo material têxtil, para tratar fibra ou polpa derivada de madeira e para melhorar a qualidade de ração animal
ES2368165T3 (es) 2005-08-04 2011-11-15 Novozymes, Inc. Polipéptidos con actividad de beta-glucosidasa y polinucleótidos que codifican los mismos.
BRPI0616721A2 (pt) 2005-09-30 2011-06-28 Novozymes Inc métodos para degradar ou converter um material celulósico e para produzir uma substáncia, e, composição detergente
FI120045B (fi) 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
WO2007071820A1 (en) 2005-12-22 2007-06-28 Ab Enzymes Oy Novel enzymes
WO2008008793A2 (en) 2006-07-10 2008-01-17 Dyadic International Inc. Methods and compositions for degradation of lignocellulosic material
GB0614442D0 (en) * 2006-07-20 2006-08-30 Fluxome Sciences As Metabolically engineered cells for the production of pinosylvin
US8546106B2 (en) 2006-07-21 2013-10-01 Novozymes, Inc. Methods of increasing secretion of polypeptides having biological activity
US8680252B2 (en) * 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
RU2510417C2 (ru) 2007-05-31 2014-03-27 Новозаймз, Инк. Способы повышения усиливающей целлюлолитической активности полипептида
WO2008148131A1 (en) 2007-05-31 2008-12-04 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP2195424B1 (en) 2007-09-28 2018-11-14 Novozymes A/S Polypeptides having cellobiohydrolase ii activity and polynucleotides encoding same
DK2195421T3 (en) 2007-09-28 2015-12-14 Novozymes As Polypeptides with acetylxylanesteraseaktivitet and polynucleotides encoding them
CN101878299A (zh) 2007-11-27 2010-11-03 诺维信公司 具有α-葡糖醛酸糖苷酶活性的多肽和编码该多肽的多核苷酸
BRPI0820647A8 (pt) 2007-11-30 2017-06-06 Novozymes As Construção de ácido nucleico, célula microbiana hospedeira recombinante, métodos para produzir o polipeptídeo tendo atividade de alfa-larabinofuranosidase, e para degradar um material 5 contendo xilano
US7851193B2 (en) 2007-12-05 2010-12-14 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
EP2224822B1 (en) 2007-12-06 2014-05-21 Novozymes A/S Polypeptides having acetylxylan esterase activity and polynucleotides encoding same
CN101939420A (zh) 2007-12-07 2011-01-05 诺维信公司 具有阿魏酸酯酶活性的多肽和编码该多肽的多核苷酸
BRPI0822031A2 (pt) 2007-12-19 2017-06-13 Novozymes As polipeptídeo e polinicleotídeo isolados, construção de ácido nucleico, célula hospedeira recombinante, métodos para produzir o polipeptídeo, um mutante de uma célula precursora, uma proteína e um produto de fermentação, para inibir a expressão de um polipeptídeo, para degradar ou converter um material celulósico, e para fermentar um material celulósico, planta transgênica, parte de planta ou célula de planta, e, molécula de rna inibidora
CN101970471A (zh) 2007-12-19 2011-02-09 诺维信公司 具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸
AU2008343105A1 (en) 2007-12-19 2009-07-09 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US8575426B2 (en) 2007-12-19 2013-11-05 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US8415119B2 (en) * 2008-02-25 2013-04-09 Novozymes A/S Method for increasing expression yield of a protein of interest
BRPI0910965A2 (pt) 2008-04-17 2016-01-05 Novozymes As polipeptídeo isolado, polinucleotídeos isolado e mutante, construto de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, métodos para produzir um polinucleotídeo e para produzir um polipeptídeo, planta transgênica, parte de planta ou célula de planta, método para degradar um material, e, composição
CA2732122A1 (en) 2008-07-29 2010-02-04 Novozymes A/S Polypeptides having alpha-glucuronidase activity and polynucleotides encoding same
BRPI0914685A2 (pt) 2008-07-31 2019-09-24 Novozymes As peptídeo e polinucleotídeo isolados, célula hospedeira recombinante, métodos para produzir o polipeptídeo, um mutante de uma célula precursora, uma proteína, e um produto de fermentação, para inibir a expressão de um polipeptídeo, para degradar ou converter e para fermentar um material celulósico ou contendo xilano, planta transgênica, parte de planta ou célula de planta, e, molécula de rna inibidora de filamento duplo
WO2010039840A1 (en) 2008-09-30 2010-04-08 Novozymes, Inc. Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum
US8058513B2 (en) 2008-11-10 2011-11-15 Novozymes, Inc. Polypeptides having feruloyl esterase activity and polynucleotides encoding same
US8805427B2 (en) 2008-11-14 2014-08-12 Microsoft Corporation Channel reuse with cognitive low interference signals
WO2010065448A1 (en) 2008-12-04 2010-06-10 Novozymes, Inc. Polypeptides having feruloyl esterase activity and polynucleotides encoding same
WO2010065830A1 (en) 2008-12-04 2010-06-10 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US8604277B2 (en) 2009-01-28 2013-12-10 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
EP2411511B1 (en) 2009-03-24 2018-08-08 Novozymes A/S Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
CN102482680B (zh) 2009-04-30 2014-09-10 诺维信股份有限公司 具有木聚糖酶活性的多肽和编码该多肽的多核苷酸
WO2010138754A1 (en) 2009-05-29 2010-12-02 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
WO2010141325A1 (en) 2009-06-02 2010-12-09 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US8143021B2 (en) 2009-07-07 2012-03-27 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2011035027A2 (en) 2009-09-17 2011-03-24 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2011035029A1 (en) 2009-09-18 2011-03-24 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
DK2483295T3 (en) 2009-09-29 2016-02-22 Novozymes Inc Polypeptides having cellulolytic enhancing activity and polynucleotides encoding them
US8211665B2 (en) 2009-09-29 2012-07-03 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
CN108165542A (zh) 2009-09-30 2018-06-15 诺维信股份有限公司 具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸
CN102666846A (zh) 2009-09-30 2012-09-12 诺维信公司 具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸
US8541651B2 (en) * 2009-10-23 2013-09-24 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
CA2776336A1 (en) 2009-10-29 2011-05-19 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP2496692B1 (en) 2009-11-06 2016-03-16 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
MX2012005152A (es) 2009-11-06 2012-06-12 Novozymes Inc Composiciones para sacrificacion de material celulosico.
MX350399B (es) 2009-12-18 2017-09-06 Novozymes Inc Metodos para producir polipeptidos en mutantes de trichoderma deficientes en proteasa.
EP2371963A1 (en) * 2010-03-29 2011-10-05 Heinrich-Heine-Universität Düsseldorf Protein production in filamentous fungi
US8686218B2 (en) * 2010-04-14 2014-04-01 The Penn State Research Foundation Strategies for the transgenic manipulation of filamentous fungi
AU2011336923B2 (en) * 2010-11-22 2017-02-16 Cargill, Incorporated Compositions and methods for 3-hydroxypropionic acid production
JP6067564B2 (ja) * 2011-08-12 2017-01-25 国立大学法人東北大学 免疫応答を回避するための薬剤
AU2012298799B2 (en) * 2011-08-24 2018-02-01 Novozymes, Inc. Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
CN103748226A (zh) * 2011-08-24 2014-04-23 诺维信股份有限公司 获得丝状真菌宿主细胞的阳性转化体的方法
US20130109055A1 (en) * 2011-10-31 2013-05-02 Bp Corporation North America Inc. Use of mammalian promoters in filamentous fungi
US20140303036A1 (en) * 2011-11-23 2014-10-09 Dsm Ip Assets B.V. Nucleic Acid Assembly System
EP2808388B1 (en) * 2012-01-23 2019-03-13 AGC Inc. Expression vector and method for producing protein
US10023872B2 (en) * 2012-12-21 2018-07-17 Novozymes, Inc. Methods for producing heterologous polypeptides in mutants of trichoderma
CN110628749A (zh) * 2013-03-08 2019-12-31 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
WO2014145768A2 (en) * 2013-03-15 2014-09-18 Bp Corporation North America Inc. Use of non-fungal 5' utrs in filamentous fungi
EP2840139A1 (en) * 2013-08-23 2015-02-25 Wageningen Universiteit Method for the single step introduction of a plurality of genes in microbial cells
WO2015187935A1 (en) * 2014-06-06 2015-12-10 Novozymes A/S Enzyme compositions and uses thereof
DK3183343T3 (da) * 2014-08-20 2020-03-23 Novozymes As Rekombinase-medieret integration af et polynukleotidbibliotek
WO2016145084A1 (en) * 2015-03-09 2016-09-15 Novozymes A/S Methods of introducing multiple expression constructs into a eukaryotic cell
CN110431224A (zh) * 2017-03-13 2019-11-08 帝斯曼知识产权资产管理有限公司 锌双核簇转录调控因子缺陷型菌株
KR20200026878A (ko) * 2017-06-06 2020-03-11 지머젠 인코포레이티드 균류 균주를 개량하기 위한 htp 게놈 공학 플랫폼

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101535340A (zh) * 2006-05-19 2009-09-16 格利科菲公司 促红细胞生成素组合物
CN101970651A (zh) * 2007-05-31 2011-02-09 诺维信股份有限公司 用于降解纤维素材料的组合物
WO2010039889A2 (en) * 2008-09-30 2010-04-08 Novozymes, Inc. Methods for using positively and negatively selectable genes in a filamentous fungal cell

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107429255A (zh) * 2015-03-09 2017-12-01 诺维信公司 将多种表达构建体引入真核细胞的方法
CN107429255B (zh) * 2015-03-09 2022-01-25 诺维信公司 将多种表达构建体引入真核细胞的方法
CN113302305A (zh) * 2018-12-12 2021-08-24 诺维信公司 提高丝状真菌细胞在多肽的产生方面的生产力的方法
CN110724643A (zh) * 2019-11-18 2020-01-24 河南省农业科学院植物保护研究所 一株具有防病促生功能的橘灰青霉及其菌剂和应用
CN110724643B (zh) * 2019-11-18 2022-07-15 河南省农业科学院植物保护研究所 一株具有防病促生功能的橘灰青霉及其菌剂和应用

Also Published As

Publication number Publication date
WO2013028912A3 (en) 2013-06-06
US10822601B2 (en) 2020-11-03
US20140212977A1 (en) 2014-07-31
EP2748322A2 (en) 2014-07-02
US20170015986A1 (en) 2017-01-19
WO2013028912A2 (en) 2013-02-28
US10301610B2 (en) 2019-05-28
AU2012298799B2 (en) 2018-02-01
IN2014CN02136A (enExample) 2015-05-29
US20190249161A1 (en) 2019-08-15
US9493790B2 (en) 2016-11-15
BR112014004190A2 (pt) 2017-03-01
CN109136114A (zh) 2019-01-04
AU2012298799A1 (en) 2014-02-13

Similar Documents

Publication Publication Date Title
US10822601B2 (en) Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
US12195776B2 (en) Cellulolytic enzyme compositions and uses thereof
AU2019206043B2 (en) Enzyme compositions and uses thereof
JP5651466B2 (ja) 非相同及び相同のセルラーゼ発現システム
US20220064228A1 (en) Methods For Increasing The Productivity Of A Filamentous Fungal Cell In The Production Of A Polypeptide
US20170166939A1 (en) Enzyme Compositions and Uses Thereof
US10647972B2 (en) Methods for obtaining positive transformants of a filamentous fungal host cell

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20140625