AU2012298799B2 - Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell - Google Patents

Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell Download PDF

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AU2012298799B2
AU2012298799B2 AU2012298799A AU2012298799A AU2012298799B2 AU 2012298799 B2 AU2012298799 B2 AU 2012298799B2 AU 2012298799 A AU2012298799 A AU 2012298799A AU 2012298799 A AU2012298799 A AU 2012298799A AU 2012298799 B2 AU2012298799 B2 AU 2012298799B2
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homologous
cellobiohydrolase
seq
gene
mature polypeptide
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AU2012298799A1 (en
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Qiming Jin
Debbie Yaver
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Novozymes Inc
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Novozymes Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0115—Oligoxyloglucan reducing-end-specific cellobiohydrolase (3.2.1.150)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2012298799A 2011-08-24 2012-08-23 Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell Active AU2012298799B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161526809P 2011-08-24 2011-08-24
US61/526,809 2011-08-24
PCT/US2012/052143 WO2013028912A2 (en) 2011-08-24 2012-08-23 Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell

Publications (2)

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AU2012298799A1 AU2012298799A1 (en) 2014-02-13
AU2012298799B2 true AU2012298799B2 (en) 2018-02-01

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US (3) US9493790B2 (enExample)
EP (1) EP2748322A2 (enExample)
CN (2) CN103890180A (enExample)
AU (1) AU2012298799B2 (enExample)
BR (1) BR112014004190A2 (enExample)
IN (1) IN2014CN02136A (enExample)
WO (1) WO2013028912A2 (enExample)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112014004188A2 (pt) * 2011-08-24 2017-03-21 Novozymes Inc método para obter transformantes positivos de uma célula hospedeira fúngica filamentosa, célula hospedeira fúngica filamentosa, método para produzir múltiplos polipeptídeos recombinantes, e, construto em tandem
WO2013028912A2 (en) * 2011-08-24 2013-02-28 Novozymes, Inc. Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
BR112014016587A2 (pt) 2012-01-05 2020-10-27 Novartis Ag células fúngicas filamentosas deficientes de protease e métodos de uso das mesmas
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
WO2015004241A2 (en) 2013-07-10 2015-01-15 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
US20150147774A1 (en) * 2013-11-22 2015-05-28 National University Of Singapore Expression construct for yeast and a method of using the construct
EP3152315B1 (en) * 2014-06-06 2018-08-15 Novozymes A/S Enzyme compositions and uses thereof
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
US10066244B2 (en) 2014-09-23 2018-09-04 Novozymes A/S Process for producing ethanol and fermenting organisms
US10385352B2 (en) 2015-03-09 2019-08-20 Novozymes A/S Methods of introducing multiple expression constructs into a eukaryotic cell
BR112018067371A2 (pt) 2016-03-02 2019-01-15 Novozymes As variante de celobio-hidrolase, composição de enzimas, formulação de caldo inteiro ou composição de cultura de células, polinucleotídeo, célula hospedeira recombinante, planta, parte de planta ou célula de planta transgênica, métodos para produção e para obtenção de uma variante de celobio-hidrolase, planta trangênica, e, processos para degradação de um material celulósico, para produção de um produto de fermentação e para fermentação de um material celulósico
WO2018093752A1 (en) * 2016-11-15 2018-05-24 Danisco Us Inc. Filamentous fungi with improved protein production
WO2018106792A1 (en) 2016-12-06 2018-06-14 Novozymes A/S Improved processes for production of ethanol from xylose-containing cellulosic substrates using engineered yeast strains
WO2018220116A1 (en) 2017-05-31 2018-12-06 Novozymes A/S Xylose fermenting yeast strains and processes thereof for ethanol production
AU2018275794A1 (en) 2017-06-02 2019-12-05 Novozymes A/S Improved yeast for ethanol production
CN109988714B (zh) * 2017-12-29 2021-12-28 青岛蔚蓝生物集团有限公司 一种里氏木霉及其应用
MX2020007914A (es) 2018-01-29 2020-10-28 Novozymes As Microorganismos con uso de nitrogeno mejorado para produccion de etanol.
CN112601818A (zh) 2018-07-25 2021-04-02 诺维信公司 用于生产乙醇的表达酶的酵母
WO2020076697A1 (en) 2018-10-08 2020-04-16 Novozymes A/S Enzyme-expressing yeast for ethanol production
WO2020123845A1 (en) * 2018-12-12 2020-06-18 Novozymes A/S Methods for increasing the productivity of a filamentous fungal cell in the production of a polypeptide
MX2021015821A (es) 2019-07-26 2022-02-03 Novozymes As Microorganismos con transporte de nitrogeno mejorado para la produccion de etanol.
MX2021015818A (es) 2019-08-06 2022-02-03 Novozymes As Proteinas de fusion para mejorar la expresion de enzimas.
CN110724643B (zh) * 2019-11-18 2022-07-15 河南省农业科学院植物保护研究所 一株具有防病促生功能的橘灰青霉及其菌剂和应用
CA3158982A1 (en) 2019-12-10 2021-06-17 Monica TASSONE Microorganism for improved pentose fermentation
AU2021338555A1 (en) 2020-09-04 2023-03-09 Microbiogen Pty. Ltd. Improved fermenting organism for ethanol production
BR112023025624A2 (pt) 2021-06-07 2024-02-27 Novozymes As Célula de levedura recombinante, célula hospedeira recombinante, composição, cocultura, métodos de produção de um derivado de uma célula hospedeira recombinante e de produção de um produto de fermentação, e, uso de uma célula hospedeira recombinante
CN114561303B (zh) * 2022-02-14 2023-11-17 大连理工大学 一株分泌高性能纤维素酶的里氏木霉工程菌株及其应用
CN115651933A (zh) * 2022-06-06 2023-01-31 福州市皮肤病防治院 高效精准替换红色毛癣菌基因的方法
CN120344672A (zh) 2022-09-23 2025-07-18 诺维信公司 用于乙醇生产的改进发酵生物
AU2024367272A1 (en) 2023-10-25 2026-04-09 Microbiogen Pty. Ltd Improved fermenting organism for ethanol production
CN117757643B (zh) * 2024-01-12 2025-06-13 华东理工大学 一种分泌表达人溶菌酶的里氏木霉工程菌及其构建方法和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994003072A1 (en) * 1992-07-31 1994-02-17 Panlabs Incorporated Recombinant cells, dna constructs, vectors and methods for expressing phytate degrading enzymes in desired ratios
WO2007136865A2 (en) * 2006-05-19 2007-11-29 Glycofi, Inc Recombinant vectors
WO2008009728A1 (en) * 2006-07-20 2008-01-24 Fluxome Sciences A/S Metabolically engineered cells for the production of pinosylvin
US20080299613A1 (en) * 2007-05-31 2008-12-04 Novozymes, Inc. Compositions for degrading cellulosic material

Family Cites Families (114)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI864473A7 (fi) * 1985-11-20 1987-05-21 Panlabs Inc Yhdistetyt ilmaisukasetit fungaalitransformaatiota varten.
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5989870A (en) 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
US5776757A (en) 1988-03-24 1998-07-07 Novo Nordisk A/S Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
EP0551386B1 (en) * 1990-10-05 2004-01-07 Genencor International, Inc. Methods for treating cotton-containing fabrics with cellulase
EP0695349B1 (en) 1993-03-10 2004-01-21 Novozymes A/S Enzymes with xylanase activity from aspergillus aculeatus
CA2191718A1 (en) 1994-06-03 1995-12-14 Randy M. Berka Phosphonyldipeptides useful in the treatment of cardiovascular diseases
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
US20030044956A1 (en) 1995-08-23 2003-03-06 Short Jay M. Enzymes having carboxymethyl cellulase activity and methods of use thereof
AU1750697A (en) 1996-01-19 1997-08-11 Novo Nordisk Biotech, Inc. Morphological mutants of filamentous fungi
JP4436934B2 (ja) * 1996-09-13 2010-03-24 ノボザイムス，インコーポレイティド 変更された量のポリペプチドを生成するｄｎａ挿入突然変異を有する細胞
US6451063B1 (en) 1996-09-25 2002-09-17 Genencor International, Inc. Cellulase for use in industrial processes
US6017870A (en) 1996-10-09 2000-01-25 Genencor International, Inc. Purified cellulase and method of producing
US5811381A (en) 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US6265204B1 (en) * 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
US6558937B1 (en) 1997-07-31 2003-05-06 Dsm N.V. Cellulose degrading enzymes of aspergillus
US5871550A (en) 1997-08-26 1999-02-16 Genencor International, Inc. Mutant Thermonospora spp. cellulase
CA2309886A1 (en) 1997-11-19 1999-05-27 Genencor International, Inc. Cellulase produced by actinomycetes and method of producing same
DE69836227T2 (de) 1997-12-16 2007-08-23 Genencor International, Inc., Palo Alto Verfahren zur herstellung egiii-ähnlicher enzyme
ES2143408B1 (es) * 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
WO1999060136A1 (en) * 1998-05-15 1999-11-25 Novo Nordisk Biotech, Inc. Methods for producing polypeptides in filamentous fungal mutant cells
EP1194572A2 (en) 1999-03-22 2002-04-10 Novozymes Biotech, Inc. Promoter sequences derived from fusarium venenatum and uses thereof
CN1509330A (zh) 2001-05-18 2004-06-30 ŵ��ø�ɷ����޹�˾ 具有纤维二糖酶活性的多肽和编码其的多核苷酸
US6982159B2 (en) 2001-09-21 2006-01-03 Genencor International, Inc. Trichoderma β-glucosidase
US7045331B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. EGVII endoglucanase and nucleic acids encoding the same
US7005289B2 (en) 2001-12-18 2006-02-28 Genencor International, Inc. BGL5 β-glucosidase and nucleic acids encoding the same
US7045332B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. BGL4 β-glucosidase and nucleic acids encoding the same
US7049125B2 (en) 2001-12-18 2006-05-23 Genencor International, Inc. EGVIII endoglucanase and nucleic acids encoding the same
US7056721B2 (en) 2001-12-18 2006-06-06 Genencor International, Inc. EGVI endoglucanase and nucleic acids encoding the same
US7972832B2 (en) 2002-08-16 2011-07-05 Danisco Us Inc. Variant Hypocrea jecorina CBH1 cellulases
US7582462B2 (en) 2002-11-07 2009-09-01 Danisco Us Inc., Genencor Division BGL6 beta-glucosidase and nucleic acids encoding the same
US7407788B2 (en) 2002-11-21 2008-08-05 Danisco A/S, Genencor Division BGL7 beta-glucosidase and nucleic acids encoding the same
CN1898381B (zh) 2003-03-21 2013-07-17 金克克国际有限公司 Cbh1同源物和cbh1纤维素酶变体
WO2005001065A2 (en) 2003-04-01 2005-01-06 Genencor International, Inc. Variant humicola grisea cbh1.1
ATE471370T1 (de) 2003-05-02 2010-07-15 Novozymes Inc Varianten von beta-glukosidasen
CA2915553A1 (en) 2003-05-29 2005-01-06 Genencor International, Inc. Trichoderma genes
DK1682656T3 (da) 2003-10-28 2013-11-18 Novozymes Inc Polypeptider med beta-glucosidaseaktivitet og polynukleotider, der koder for dem
DK1713825T3 (da) 2004-01-30 2012-11-05 Novozymes Inc Polypeptider med cellulolytisk forøgende aktivitet og polynukleotider der koder for samme
DK1733033T3 (da) 2004-02-06 2012-09-24 Novozymes Inc Polypeptider med cellulolytisk forbedrende aktivitet og polynukleotider, der koder for disse
WO2006078256A2 (en) 2004-02-12 2006-07-27 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
US8097445B2 (en) 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
EP1735447A2 (en) 2004-03-25 2006-12-27 Genencor International, Inc. Cellulase fusion protein and heterologous cellulase fusion construct encoding the same
EP1838849B1 (en) 2004-12-30 2015-08-26 Danisco US Inc. Variant hypocrea jecorina cbh2 cellulases
WO2006074435A2 (en) 2005-01-06 2006-07-13 Novozymes, Inc. Polypeptides having cellobiohydrlase activity and polynucleotides encoding same
AR053066A1 (es) 2005-04-26 2007-04-18 Novozymes As Arabinofuranosidasas
ES2458301T3 (es) 2005-04-29 2014-04-30 Ab Enzymes Oy Celulasas mejoradas
CN101287751A (zh) 2005-08-04 2008-10-15 诺维信股份有限公司 具有β-葡糖苷酶活性的多肽和编码该多肽的多核苷酸
BRPI0616721A2 (pt) 2005-09-30 2011-06-28 Novozymes Inc métodos para degradar ou converter um material celulósico e para produzir uma substáncia, e, composição detergente
FI120045B (fi) 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
EP1969123B1 (en) 2005-12-22 2017-07-12 AB Enzymes Oy Novel enzymes
US8304212B2 (en) 2006-07-10 2012-11-06 Dyadic International, Inc. Methods and compositions for degradation of lignocellulosic material
WO2008057637A2 (en) 2006-07-21 2008-05-15 Novozymes, Inc. Methods of increasing secretion of polypeptides having biological activity
EP2505651A3 (en) * 2006-12-10 2013-01-09 Dyadic International, Inc. Isolated fungus with reduced protease activity
RU2510417C2 (ru) 2007-05-31 2014-03-27 Новозаймз, Инк. Способы повышения усиливающей целлюлолитической активности полипептида
EP2069489A1 (en) 2007-05-31 2009-06-17 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP2195421B1 (en) 2007-09-28 2015-09-16 Novozymes A/S Polypeptides having acetylxylan esterase activity and polynucleotides encoding same
US8124394B2 (en) 2007-09-28 2012-02-28 Novozymes, A/S Polypeptides having cellobiohydrolase II activity and polynuleotides encoding same
US7867745B2 (en) 2007-11-27 2011-01-11 Novozymes A/S Polypeptides having alpha-glucuronidase activity and polynucleotides encoding same
DK2238243T3 (en) 2007-11-30 2019-04-15 Novozymes As Polypeptides with arabinofuranosidase activity and polynucleotides encoding them
US7851193B2 (en) 2007-12-05 2010-12-14 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
ES2490608T3 (es) 2007-12-06 2014-09-04 Novozymes A/S Polipéptidos con actividad de esterasa de acetilxilano y polinucleótidos que codifican los mismos
EP2225370A1 (en) 2007-12-07 2010-09-08 Novozymes A/S Polypeptides having feruloyl esterase activity and polynucleotides encoding same
EP2245050A2 (en) 2007-12-19 2010-11-03 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
BRPI0822031A2 (pt) 2007-12-19 2017-06-13 Novozymes As polipeptídeo e polinicleotídeo isolados, construção de ácido nucleico, célula hospedeira recombinante, métodos para produzir o polipeptídeo, um mutante de uma célula precursora, uma proteína e um produto de fermentação, para inibir a expressão de um polipeptídeo, para degradar ou converter um material celulósico, e para fermentar um material celulósico, planta transgênica, parte de planta ou célula de planta, e, molécula de rna inibidora
EP2235173A2 (en) 2007-12-19 2010-10-06 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CA2709367A1 (en) 2007-12-19 2009-07-09 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN101952303A (zh) * 2008-02-25 2011-01-19 诺维信公司 提高目标蛋白质的表达产率的方法
WO2009127729A1 (en) 2008-04-17 2009-10-22 Novozymes A/S Polypeptides having ferulic acid esterase activity and polynucleotides encoding same
US8735128B2 (en) 2008-07-29 2014-05-27 Novozymes A/S Polypeptides having alpha-glucuronidase activity and polynucleotides encoding same
CA2732099A1 (en) 2008-07-31 2010-02-04 Novozymes A/S Polypeptides having acetylxylan esterase activity and polynucleotides encoding same
CN105586355B (zh) 2008-09-30 2020-05-22 诺维信股份有限公司 在镶片镰孢的酶缺陷突变体中产生多肽的方法
JP2012504390A (ja) * 2008-09-30 2012-02-23 ノボザイムス，インコーポレイティド 糸状菌細胞におけるポジティブ及びネガティブ選択遺伝子の使用方法
US8058513B2 (en) 2008-11-10 2011-11-15 Novozymes, Inc. Polypeptides having feruloyl esterase activity and polynucleotides encoding same
US8805427B2 (en) 2008-11-14 2014-08-12 Microsoft Corporation Channel reuse with cognitive low interference signals
WO2010065448A1 (en) 2008-12-04 2010-06-10 Novozymes, Inc. Polypeptides having feruloyl esterase activity and polynucleotides encoding same
US7771983B2 (en) 2008-12-04 2010-08-10 Novozymos, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2010088387A1 (en) 2009-01-28 2010-08-05 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
CN102365360A (zh) 2009-03-24 2012-02-29 诺维信公司 具有乙酰木聚糖酯酶活性的多肽和编码该多肽的多核苷酸
CN102482680B (zh) 2009-04-30 2014-09-10 诺维信股份有限公司 具有木聚糖酶活性的多肽和编码该多肽的多核苷酸
EP2435561B1 (en) 2009-05-29 2018-08-08 Novozymes Inc. Methods for enhancing the degradation or conversion of cellulosic material
ES2534078T3 (es) 2009-06-02 2015-04-17 Novozymes Inc. Polipéptidos con actividad de celobiohidrolasa y polinucleótidos que codifican los mismos
CA2767169A1 (en) 2009-07-07 2011-01-13 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
DK3269804T3 (da) 2009-09-17 2020-11-23 Novozymes Inc Polypeptider med cellulolyseforbedrende aktivitet og polynukleotider, som koder for dem
EP2478095A1 (en) 2009-09-18 2012-07-25 Novozymes Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
WO2011041397A1 (en) 2009-09-29 2011-04-07 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CA2775347A1 (en) 2009-09-29 2011-04-07 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
BR112012007375A2 (pt) 2009-09-30 2016-11-22 Novozymes As polipeptídeo isolado, polinucleotídeo isolado, métodos para produzir um polipeptídeo, para produzir um mutante de uma célula precursora, para inibir a expressão de um polipeptídeo, para produzir uma proteína, para degradar ou converter um material celulósico, para produzir um produto de fermentação e para fermentar um material celulósico, planta transgênica, parte de planta ou célula de planta transformada, molécula de rna inibitória de filamento duplo, e, composição detergente
WO2011039319A1 (en) 2009-09-30 2011-04-07 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2011050037A1 (en) * 2009-10-23 2011-04-28 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
WO2011059740A1 (en) 2009-10-29 2011-05-19 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
DK2496692T3 (en) 2009-11-06 2016-06-27 Novozymes Inc POLYPEPTIDES WITH xylanase AND POLYNUCLEOTIDES ENCODING THEM
DK3222716T3 (da) 2009-11-06 2020-11-16 Novozymes Inc Sammensætninger til saccharificering af cellulosemateriale
MX350399B (es) 2009-12-18 2017-09-06 Novozymes Inc Metodos para producir polipeptidos en mutantes de trichoderma deficientes en proteasa.
EP2371963A1 (en) * 2010-03-29 2011-10-05 Heinrich-Heine-Universität Düsseldorf Protein production in filamentous fungi
WO2011130247A2 (en) * 2010-04-14 2011-10-20 The Penn State Research Foundation Strategies for the transgenic manipulation of filamentous fungi
CN103502432A (zh) * 2010-11-22 2014-01-08 诺维信股份有限公司 用于生产3-羟基丙酸的组合物和方法
JP6067564B2 (ja) * 2011-08-12 2017-01-25 国立大学法人東北大学 免疫応答を回避するための薬剤
BR112014004188A2 (pt) * 2011-08-24 2017-03-21 Novozymes Inc método para obter transformantes positivos de uma célula hospedeira fúngica filamentosa, célula hospedeira fúngica filamentosa, método para produzir múltiplos polipeptídeos recombinantes, e, construto em tandem
WO2013028912A2 (en) * 2011-08-24 2013-02-28 Novozymes, Inc. Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
US20130109055A1 (en) * 2011-10-31 2013-05-02 Bp Corporation North America Inc. Use of mammalian promoters in filamentous fungi
EP2783000A1 (en) * 2011-11-23 2014-10-01 DSM IP Assets B.V. Nucleic acid assembly system
WO2013111754A1 (ja) * 2012-01-23 2013-08-01 旭硝子株式会社 発現ベクターおよび蛋白質の製造方法
EP2935609A1 (en) * 2012-12-21 2015-10-28 Novozymes, Inc. Methods for producing heterologous polypeptides in mutants of trichoderma
CN105164254B (zh) * 2013-03-08 2019-06-28 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
WO2014145768A2 (en) * 2013-03-15 2014-09-18 Bp Corporation North America Inc. Use of non-fungal 5' utrs in filamentous fungi
EP2840139A1 (en) * 2013-08-23 2015-02-25 Wageningen Universiteit Method for the single step introduction of a plurality of genes in microbial cells
EP3152315B1 (en) * 2014-06-06 2018-08-15 Novozymes A/S Enzyme compositions and uses thereof
WO2016026938A1 (en) * 2014-08-20 2016-02-25 Novozymes A/S Recombinase-mediated integration of a polynucleotide library
US10385352B2 (en) * 2015-03-09 2019-08-20 Novozymes A/S Methods of introducing multiple expression constructs into a eukaryotic cell
EP3596199A1 (en) * 2017-03-13 2020-01-22 DSM IP Assets B.V. Zinc binuclear cluster transcriptional regulator-deficient strain
JP7227162B2 (ja) * 2017-06-06 2023-02-21 ザイマージェン インコーポレイテッド 真菌株を改良するためのｈｔｐゲノム操作プラットフォーム

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994003072A1 (en) * 1992-07-31 1994-02-17 Panlabs Incorporated Recombinant cells, dna constructs, vectors and methods for expressing phytate degrading enzymes in desired ratios
WO2007136865A2 (en) * 2006-05-19 2007-11-29 Glycofi, Inc Recombinant vectors
WO2008009728A1 (en) * 2006-07-20 2008-01-24 Fluxome Sciences A/S Metabolically engineered cells for the production of pinosylvin
US20080299613A1 (en) * 2007-05-31 2008-12-04 Novozymes, Inc. Compositions for degrading cellulosic material

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BJARNE G HANSEN ET AL, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 77, no. 9, doi:10.1128/AEM.01768-10, ISSN 0099-2240, (20110501), pages 3044 - 3051, (20110311) *
GOUKA, R.J. et al., Nature Biotechnology, 1999, Vol. 17, pages 598-601 *
KARHUNEN T ET AL, MOLECULAR & GENERAL GENETICS : MGG DEC 1993, (199312), vol. 241, no. 5-6, ISSN 0026-8925, pages 515 - 522, *
KERANEN SIRKKA ET AL, CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, (19950101), vol. 6, no. 5, doi:10.1016/0958-1669(95)80088-3, ISSN 0958-1669, pages 534 - 537, *
KIMURA, N. et al., Journal of Bacteriology, 1993, Vol. 175, pages 4427-4435 *
SHARMA, K.K. et al., Biotechnology and Applied Biochemistry, 2006, Vol. 43, pages 181-186 *

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