IN2014CN02136A - - Google Patents

Download PDF

Info

Publication number
IN2014CN02136A
IN2014CN02136A IN2136CHN2014A IN2014CN02136A IN 2014CN02136 A IN2014CN02136 A IN 2014CN02136A IN 2136CHN2014 A IN2136CHN2014 A IN 2136CHN2014A IN 2014CN02136 A IN2014CN02136 A IN 2014CN02136A
Authority
IN
India
Prior art keywords
biological activity
relates
present
filamentous fungal
recombinant polypeptides
Prior art date
Application number
Inventor
Debbie Yaver
Qiming Jin
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Publication of IN2014CN02136A publication Critical patent/IN2014CN02136A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0115Oligoxyloglucan reducing-end-specific cellobiohydrolase (3.2.1.150)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to methods for constructing a filamentous fungal strain for production of multiple recombinant polypeptides having biological activity. The present invention also relates to methods for producing multiple recombinant polypeptides having biological activity in a filamentous fungal strain. The present invention also relates to filamentous fungal strains expressing multiple recombinant polypeptides having biological activity.
IN2136CHN2014 2011-08-24 2012-08-23 IN2014CN02136A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161526809P 2011-08-24 2011-08-24
PCT/US2012/052143 WO2013028912A2 (en) 2011-08-24 2012-08-23 Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell

Publications (1)

Publication Number Publication Date
IN2014CN02136A true IN2014CN02136A (en) 2015-05-29

Family

ID=47358264

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2136CHN2014 IN2014CN02136A (en) 2011-08-24 2012-08-23

Country Status (7)

Country Link
US (3) US9493790B2 (en)
EP (1) EP2748322A2 (en)
CN (2) CN103890180A (en)
AU (1) AU2012298799B2 (en)
BR (1) BR112014004190A2 (en)
IN (1) IN2014CN02136A (en)
WO (1) WO2013028912A2 (en)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9404101B2 (en) * 2011-08-24 2016-08-02 Novozymes, Inc. Methods for obtaining positive transformants of a filamentous fungal host cell
WO2013028912A2 (en) * 2011-08-24 2013-02-28 Novozymes, Inc. Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
RU2645252C2 (en) 2012-01-05 2018-02-19 Глюкос Финланд Ой Felamentous fungi cell with protease deficit and methods of its application
US9695454B2 (en) 2012-05-23 2017-07-04 Glykos Finland Oy Production of fucosylated glycoproteins
CA2916905A1 (en) 2013-07-10 2015-01-15 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
US20150147774A1 (en) * 2013-11-22 2015-05-28 National University Of Singapore Expression construct for yeast and a method of using the construct
DK3152315T3 (en) 2014-06-06 2018-11-26 Novozymes As ENZYME COMPOSITIONS AND APPLICATIONS THEREOF
CN108064266A (en) 2014-07-21 2018-05-22 格利科斯芬兰公司 The preparation of the glycoprotein with mammal sample N- glycan in filamentous fungi
WO2016045569A1 (en) 2014-09-23 2016-03-31 Novozymes A/S Processes for producing ethanol and fermenting organisms
US10385352B2 (en) 2015-03-09 2019-08-20 Novozymes A/S Methods of introducing multiple expression constructs into a eukaryotic cell
CN109415712A (en) 2016-03-02 2019-03-01 诺维信公司 Cellobiohydrolase variant and the polynucleotides for encoding them
WO2018093752A1 (en) * 2016-11-15 2018-05-24 Danisco Us Inc. Filamentous fungi with improved protein production
CN110168095A (en) 2016-12-06 2019-08-23 诺维信公司 For using engineered yeast bacterial strain from the ameliorative way of the cellulose matrix production ethyl alcohol containing xylose
WO2018220116A1 (en) 2017-05-31 2018-12-06 Novozymes A/S Xylose fermenting yeast strains and processes thereof for ethanol production
CA3064042A1 (en) 2017-06-02 2018-12-06 Novozymes A/S Improved yeast for ethanol production
CN109988714B (en) * 2017-12-29 2021-12-28 青岛蔚蓝生物集团有限公司 Trichoderma reesei and application thereof
BR112020015348A2 (en) 2018-01-29 2020-12-08 Novozymes A/S MICRO-ORGANISMS WITH THE USE OF IMPROVED NITROGEN FOR ETHANOL PRODUCTION
CN112601818A (en) 2018-07-25 2021-04-02 诺维信公司 Enzyme-expressing yeast for producing ethanol
MX2021003955A (en) 2018-10-08 2021-05-27 Novozymes As Enzyme-expressing yeast for ethanol production.
EP3894565A1 (en) * 2018-12-12 2021-10-20 Novozymes A/S Methods for increasing the productivity of a filamentous fungal cell in the production of a polypeptide
WO2021021458A1 (en) 2019-07-26 2021-02-04 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
MX2021015818A (en) 2019-08-06 2022-02-03 Novozymes As Fusion proteins for improved enzyme expression.
CN110724643B (en) * 2019-11-18 2022-07-15 河南省农业科学院植物保护研究所 Penicillium citrinum with disease prevention and growth promotion functions, microbial inoculum and application thereof
BR112022011271A2 (en) 2019-12-10 2022-09-06 Novozymes As RECOMBINANT HOST CELL, COMPOSITION, METHODS FOR PRODUCING A CELL DERIVATIVE AND FERMENTATION PRODUCT, AND, USE OF A RECOMBINANT HOST CELL
US20230332188A1 (en) 2020-09-04 2023-10-19 Novozymes A/S Improved fermenting organism for ethanol production
EP4352241A1 (en) 2021-06-07 2024-04-17 Novozymes A/S Engineered microorganism for improved ethanol fermentation
CN114561303B (en) * 2022-02-14 2023-11-17 大连理工大学 Trichoderma reesei engineering strain secreting high-performance cellulase and application thereof
WO2024064901A2 (en) 2022-09-23 2024-03-28 Novozymes A/S Improved fermenting organism for ethanol production

Family Cites Families (118)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI864473A (en) * 1985-11-20 1987-05-21 Panlabs Inc SAMMANSATTA YTTRYCKSKASSETTER FOER FUNGALTRANSFORMERING.
DK122686D0 (en) 1986-03-17 1986-03-17 Novo Industri As PREPARATION OF PROTEINS
US5989870A (en) 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
US5648263A (en) 1988-03-24 1997-07-15 Novo Nordisk A/S Methods for reducing the harshness of a cotton-containing fabric
DK115890D0 (en) 1990-05-09 1990-05-09 Novo Nordisk As ENZYME
EP1416045A1 (en) 1990-10-05 2004-05-06 Genencor International, Inc. Composition containing a fungal cellulase for the treatment of cotton-containing fabrics
JPH09505201A (en) * 1992-07-31 1997-05-27 パンラブス インコーポレイテッド Method for expressing recombinant cell, DNA structure, vector and phytate degrading enzyme in arbitrary ratio
DE69433499T2 (en) 1993-03-10 2004-12-02 Novozymes A/S ENZYME WITH XYLANASE ACTIVITY FROM ASPERGILLUS ACULEATUS
AU694954B2 (en) 1994-06-03 1998-08-06 Novo Nordisk A/S Purified myceliophthora laccases and nucleic acids encoding same
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
CN1151762A (en) 1994-06-30 1997-06-11 诺沃诺尔迪斯克生物技术有限公司 Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US20030044956A1 (en) 1995-08-23 2003-03-06 Short Jay M. Enzymes having carboxymethyl cellulase activity and methods of use thereof
DE69733121T2 (en) 1996-01-19 2006-03-02 Novozymes Biotech, Inc., Davis MORPHOLOGICAL MUTANTS OF FILAMENTOUS MUSHROOMS
EP0966521A1 (en) * 1996-09-13 1999-12-29 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide
US6451063B1 (en) 1996-09-25 2002-09-17 Genencor International, Inc. Cellulase for use in industrial processes
US6017870A (en) 1996-10-09 2000-01-25 Genencor International, Inc. Purified cellulase and method of producing
US5811381A (en) 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US6265204B1 (en) * 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
AU739445B2 (en) 1997-07-31 2001-10-11 Dsm Ip Assets B.V. Cellulose degrading enzymes of aspergillus
US5871550A (en) 1997-08-26 1999-02-16 Genencor International, Inc. Mutant Thermonospora spp. cellulase
ES2267200T3 (en) 1997-11-19 2007-03-01 Genencor International, Inc. CELLULOSE PRODUCED BY ACTINOMYCETES AND METHOD TO PRODUCE IT.
CA2315017C (en) 1997-12-16 2011-10-11 Genencor International, Inc. Novel egiii-like enzymes, dna encoding such enzymes and methods for producing such enzymes
ES2143408B1 (en) * 1998-04-02 2000-12-01 Urquima Sa PROMOTER AND CONSTRUCTIONS FOR THE EXPRESSION OF RECOMBINANT PROTEINS IN FILAMENTAL FUNGI.
EP1078080A1 (en) * 1998-05-15 2001-02-28 Novo Nordisk Biotech, Inc. Methods for producing polypeptides in filamentous fungal mutant cells
JP4620253B2 (en) 1999-03-22 2011-01-26 ノボザイムス,インコーポレイティド Promoter for gene expression in fungal cells
JP2004527261A (en) 2001-05-18 2004-09-09 ノボザイムス アクティーゼルスカブ Polypeptide having cellobiase activity and polynucleotide encoding the same
US6982159B2 (en) 2001-09-21 2006-01-03 Genencor International, Inc. Trichoderma β-glucosidase
US7045331B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. EGVII endoglucanase and nucleic acids encoding the same
US7056721B2 (en) 2001-12-18 2006-06-06 Genencor International, Inc. EGVI endoglucanase and nucleic acids encoding the same
US7049125B2 (en) 2001-12-18 2006-05-23 Genencor International, Inc. EGVIII endoglucanase and nucleic acids encoding the same
US7045332B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. BGL4 β-glucosidase and nucleic acids encoding the same
US7005289B2 (en) 2001-12-18 2006-02-28 Genencor International, Inc. BGL5 β-glucosidase and nucleic acids encoding the same
CA2865180A1 (en) 2002-08-16 2004-02-26 Genencor International, Inc. Novel variant hyprocrea jecorina cbh1 cellulases
US7582462B2 (en) 2002-11-07 2009-09-01 Danisco Us Inc., Genencor Division BGL6 beta-glucosidase and nucleic acids encoding the same
US7407788B2 (en) 2002-11-21 2008-08-05 Danisco A/S, Genencor Division BGL7 beta-glucosidase and nucleic acids encoding the same
EP2298876A1 (en) 2003-03-21 2011-03-23 Genencor International, Inc. Novel cbh1 homologs and variant cbh1 cellulases
US7459299B2 (en) 2003-04-01 2008-12-02 Danisco A/S, Genencor Division Variant Humicola grisea CBH1.1
ES2347658T3 (en) 2003-05-02 2010-11-03 Novozymes Inc. BETA-GLUCOSIDASE VARIANTS.
EP1862540B1 (en) 2003-05-29 2011-09-14 Genencor International, Inc. Novel trichoderma genes
CN1902310B (en) 2003-10-28 2011-09-21 诺维信股份有限公司 Polypeptides having beta-glucosidase activity and polynucleotides encoding same
ES2468366T3 (en) 2004-01-30 2014-06-16 Novozymes Inc. Polypeptides with cellulolytic enhancement activity and polynucleotides that encode them
EP1733033B1 (en) 2004-02-06 2012-06-20 Novozymes Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP1715736A4 (en) * 2004-02-12 2008-09-03 Novozymes Inc Polypeptides having xylanase activity and polynucleotides encoding same
EP1735447A2 (en) 2004-03-25 2006-12-27 Genencor International, Inc. Cellulase fusion protein and heterologous cellulase fusion construct encoding the same
US8097445B2 (en) 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
DK2292747T3 (en) 2004-12-30 2017-04-24 Danisco Us Inc CBH2 CELLULASE VARIETIES OF HYPOCREA JECORINA
MX2007008050A (en) 2005-01-06 2007-08-21 Novozymes Inc Polypeptides having cellobiohydrlase activity and polynucleotides encoding same.
AR053066A1 (en) 2005-04-26 2007-04-18 Novozymes As ARABINOFURANOSIDASAS
BRPI0609906A8 (en) 2005-04-29 2017-11-21 Ab Enzymes Oy cellulase fusion protein, its production process and detergent composition containing it, expression vector, host cell, enzyme preparation, fading and bio-finishing processes and methods of treating cellulosic fiber containing textile material to treat fiber or pulp derived from wood and to improve the quality of animal feed
DK1928901T3 (en) 2005-08-04 2011-10-03 Novozymes Inc Polypeptides that have beta-glucosidase activity and same-polynucleotides
US7608689B2 (en) 2005-09-30 2009-10-27 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
ES2637008T3 (en) 2005-12-22 2017-10-10 Ab Enzymes Oy New enzymes
FI120045B (en) 2005-12-22 2009-06-15 Roal Oy Treatment of cellulose materials and enzymes useful therein
CA2651456A1 (en) * 2006-05-19 2007-11-29 Glycofi, Inc. Recombinant vectors
US8304212B2 (en) 2006-07-10 2012-11-06 Dyadic International, Inc. Methods and compositions for degradation of lignocellulosic material
GB0614442D0 (en) * 2006-07-20 2006-08-30 Fluxome Sciences As Metabolically engineered cells for the production of pinosylvin
US8546106B2 (en) 2006-07-21 2013-10-01 Novozymes, Inc. Methods of increasing secretion of polypeptides having biological activity
EP2102366A4 (en) * 2006-12-10 2010-01-27 Dyadic International Inc Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi
NZ581257A (en) * 2007-05-31 2012-03-30 Novozymes Inc Compositions for degrading cellulosic material
BRPI0812276A2 (en) 2007-05-31 2014-10-14 Novozymes Inc METHODS FOR INCREASING THE ACTIVITY OF A POLYPEPTIDE HAVING INTENSIFYING CELLULolytic ACTIVITY, DEGRADING OR CONVINCING A CELLULOSE CONTAINING MATERIAL, AND FOR MAKING A FERMENTATION PRODUCT, AND, COMPOSITION
CN101784659B (en) 2007-05-31 2016-05-18 诺维信股份有限公司 There are polypeptide and its polynucleotides of coding of cellulolytic enhancing activity
CN101903518B (en) 2007-09-28 2016-08-03 诺维信公司 There is the polypeptide of acetyl xylan esterase activity and encode the polynucleotide of this polypeptide
EP2195424B1 (en) 2007-09-28 2018-11-14 Novozymes A/S Polypeptides having cellobiohydrolase ii activity and polynucleotides encoding same
DK2215224T3 (en) 2007-11-27 2014-01-27 Novozymes As POLYPEPTIDES WITH ALFA-GLUCURONIDASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
US8034599B2 (en) 2007-11-30 2011-10-11 Novozymes A/S Polypeptides having arabinofuranosidase activity and polynucleotides encoding same
CA2707771A1 (en) 2007-12-05 2009-06-25 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
ES2490608T3 (en) 2007-12-06 2014-09-04 Novozymes A/S Polypeptides with acetyloxylane esterase activity and polynucleotides encoding them
CA2708268A1 (en) 2007-12-07 2009-06-18 Novozymes A/S Polypeptides having feruloyl esterase activity and polynucleotides encoding same
CN101945889A (en) 2007-12-19 2011-01-12 诺维信公司 Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
NZ598403A (en) 2007-12-19 2012-07-27 Novozymes As Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US8575426B2 (en) 2007-12-19 2013-11-05 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2009085859A2 (en) 2007-12-19 2009-07-09 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN101952303A (en) * 2008-02-25 2011-01-19 诺维信公司 Method for increasing expression yield of a protein of interest
WO2009127729A1 (en) 2008-04-17 2009-10-22 Novozymes A/S Polypeptides having ferulic acid esterase activity and polynucleotides encoding same
BRPI0914835B1 (en) 2008-07-29 2019-04-24 Novozymes A/S TRANSGENIC MICROBIAN HOST CELL, METHODS FOR PRODUCING A POLYPEPTIDE AND FOR PRODUCING PROTEIN, NUCLEIC ACID CONSTRUCTIONS, AND, EXPRESSION VECTOR.
CN102112604B (en) 2008-07-31 2015-09-30 诺维信公司 There are the polypeptide of acetyl xylan esterase activity and the polynucleotide of this polypeptide of coding
CN105586355B (en) 2008-09-30 2020-05-22 诺维信股份有限公司 Method for producing polypeptides in enzyme-deficient mutants of fusarium venenatum
WO2010039889A2 (en) 2008-09-30 2010-04-08 Novozymes, Inc. Methods for using positively and negatively selectable genes in a filamentous fungal cell
CA2743049A1 (en) 2008-11-10 2010-05-14 Kimberly Brown Polypeptides having feruloyl esterase activity and polynucleotides encoding same
US8805427B2 (en) 2008-11-14 2014-08-12 Microsoft Corporation Channel reuse with cognitive low interference signals
US8609933B2 (en) 2008-12-04 2013-12-17 Novozymes, Inc. Polypeptides having feruloyl esterase activity and polynucleotides encoding same
EP2373788A1 (en) 2008-12-04 2011-10-12 Novozymes Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2010088387A1 (en) 2009-01-28 2010-08-05 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
BRPI1013483B1 (en) 2009-03-24 2019-04-24 Novozymes A/S NUCLEIC ACID CONSTRUCTION, RECOMBINANT EXPRESSION VECTOR, TRANSGENIC MICROBIAN HOST CELL, METHODS FOR PRODUCING POLYPEPTIDE, TO DEGRADE ACTILED XYLAN AND TO PRODUCE COMPOSING PRODUCT, AND FERMENTATION PRODUCT,
CA2795934A1 (en) 2009-04-30 2010-11-04 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
EP2435561B1 (en) 2009-05-29 2018-08-08 Novozymes Inc. Methods for enhancing the degradation or conversion of cellulosic material
BRPI1012923B1 (en) 2009-06-02 2018-10-16 Novozymes As transgenic microbial host cell, methods for producing a polypeptide, for producing a mother cell mutant, for degrading or converting a cellulosic material, for producing a fermentation product, for fermenting a cellulosic material, nucleic acid construct, and, vector of expression
CN102597243B (en) 2009-07-07 2015-05-20 诺维信股份有限公司 Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP3805348A3 (en) 2009-09-17 2021-07-14 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN102712916B (en) 2009-09-18 2015-11-25 诺维信股份有限公司 There are the polypeptide of beta-glucosidase activity and the polynucleotide of this polypeptide of coding
CA2775358A1 (en) 2009-09-29 2011-04-07 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US8211665B2 (en) 2009-09-29 2012-07-03 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
CN108165542A (en) 2009-09-30 2018-06-15 诺维信股份有限公司 Polypeptide with cellulolytic enhancing activity and the polynucleotides for encoding the polypeptide
EP2483402A1 (en) 2009-09-30 2012-08-08 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN102597228A (en) * 2009-10-23 2012-07-18 诺维信股份有限公司 Cellobiohydrolase variants and polynucleotides encoding same
US20120260371A1 (en) 2009-10-29 2012-10-11 Novozymes A/S Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same
EP2496694B1 (en) 2009-11-06 2017-04-19 Novozymes, Inc. Compositions for saccharification of cellulosic material
CN104694517B (en) 2009-11-06 2019-06-28 诺维信股份有限公司 Polypeptide with xylanase activity and the polynucleotides for encoding the polypeptide
EP3000871A1 (en) 2009-12-18 2016-03-30 Novozymes, Inc. Methods for producing polypeptides in protease-deficient mutants of trichoderma
EP2371963A1 (en) * 2010-03-29 2011-10-05 Heinrich-Heine-Universität Düsseldorf Protein production in filamentous fungi
US8686218B2 (en) * 2010-04-14 2014-04-01 The Penn State Research Foundation Strategies for the transgenic manipulation of filamentous fungi
JP6061862B2 (en) * 2010-11-22 2017-01-18 カーギル,インコーポレイティド Compositions and methods for the production of 3-hydroxypropionic acid
JP6067564B2 (en) * 2011-08-12 2017-01-25 国立大学法人東北大学 Drugs to avoid immune response
US9404101B2 (en) * 2011-08-24 2016-08-02 Novozymes, Inc. Methods for obtaining positive transformants of a filamentous fungal host cell
WO2013028912A2 (en) * 2011-08-24 2013-02-28 Novozymes, Inc. Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
US20130109055A1 (en) * 2011-10-31 2013-05-02 Bp Corporation North America Inc. Use of mammalian promoters in filamentous fungi
US20140303036A1 (en) * 2011-11-23 2014-10-09 Dsm Ip Assets B.V. Nucleic Acid Assembly System
CN104066841B (en) * 2012-01-23 2018-01-02 旭硝子株式会社 The manufacture method of expression vector and protein
US10023872B2 (en) * 2012-12-21 2018-07-17 Novozymes, Inc. Methods for producing heterologous polypeptides in mutants of trichoderma
EP2964760B1 (en) * 2013-03-08 2021-05-12 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
WO2014145768A2 (en) * 2013-03-15 2014-09-18 Bp Corporation North America Inc. Use of non-fungal 5' utrs in filamentous fungi
EP2840139A1 (en) * 2013-08-23 2015-02-25 Wageningen Universiteit Method for the single step introduction of a plurality of genes in microbial cells
DK3152315T3 (en) * 2014-06-06 2018-11-26 Novozymes As ENZYME COMPOSITIONS AND APPLICATIONS THEREOF
CN106661573B (en) * 2014-08-20 2021-07-27 诺维信公司 Recombinase-mediated integration of polynucleotide libraries
US10385352B2 (en) * 2015-03-09 2019-08-20 Novozymes A/S Methods of introducing multiple expression constructs into a eukaryotic cell
CN110431224A (en) * 2017-03-13 2019-11-08 帝斯曼知识产权资产管理有限公司 Zinc binuclear cluster transcription regulatory factor deficient strain
CA3061984A1 (en) * 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving fungal strains

Also Published As

Publication number Publication date
US10301610B2 (en) 2019-05-28
US20170015986A1 (en) 2017-01-19
CN109136114A (en) 2019-01-04
US10822601B2 (en) 2020-11-03
US20140212977A1 (en) 2014-07-31
EP2748322A2 (en) 2014-07-02
AU2012298799B2 (en) 2018-02-01
US20190249161A1 (en) 2019-08-15
BR112014004190A2 (en) 2017-03-01
CN103890180A (en) 2014-06-25
US9493790B2 (en) 2016-11-15
AU2012298799A1 (en) 2014-02-13
WO2013028912A2 (en) 2013-02-28
WO2013028912A3 (en) 2013-06-06

Similar Documents

Publication Publication Date Title
IN2014CN02136A (en)
SG10201805291TA (en) Production of heterodimeric proteins
IL232048B (en) Aerosol generating system with improved aerosol production
ZA201208575B (en) Yeast production culture for the production of butanol
IN2015KN00323A (en)
WO2012068317A3 (en) Methods for producing recombinant proteins
SI3263303T1 (en) Method for generating a semifinished product for producing a composite form part, in particular a fibre compound form part
ZA201401094B (en) Process for the production of gasoline by using butanol in the gasoline pool
PL2681313T3 (en) A yeast cell for the production of terpenes and uses thereof
SI2758228T1 (en) Semi-finished fiber product, fiber composite material and method for the production thereof
MX2013007567A (en) Process for preparing polyesteretherols.
EP2889368A4 (en) Protein production accelerator
HRP20181115T1 (en) Production of isopropanol by improved recombinant strains
EP2678440A4 (en) Yeast strain for the production of proteins with modified o-glycosylation
MY197890A (en) Mutant strains of trichoderma reesei
IL229058A0 (en) A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii
PT2725098T (en) Kluyveromyces lactis yeast strain and methods for the production of sugars, ethanol, beta-galactosidase and biomass
ZA201409404B (en) Production of enzymes for ligno-cellulosic biomass
EP2868642A4 (en) Composite plate and production method therefor
IN2014MN01621A (en)
MX2014000916A (en) Process for the continuous production of polyetherols.
EP2925879A4 (en) Improved cultivation media and process for improved protein production by pichia strains
MX2014003936A (en) Method for producing dithiine tetracarboximides.
EP2865278B8 (en) Fat binder obtained from biomass resulting from beer production
EP2726614A4 (en) Improving the production of foreign proteins