CN103842517A - 胶原蛋白7及相关方法 - Google Patents

胶原蛋白7及相关方法 Download PDF

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Publication number
CN103842517A
CN103842517A CN201280047084.3A CN201280047084A CN103842517A CN 103842517 A CN103842517 A CN 103842517A CN 201280047084 A CN201280047084 A CN 201280047084A CN 103842517 A CN103842517 A CN 103842517A
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China
Prior art keywords
collagen
functional fragment
cell
collagen protein
express
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Pending
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CN201280047084.3A
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English (en)
Chinese (zh)
Inventor
M·德苏萨
M·韦斯瓦纳杉
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Lotus Tissue Repair Inc
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Lotus Tissue Repair Inc
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Priority to CN201711271805.XA priority Critical patent/CN108165593A/zh
Publication of CN103842517A publication Critical patent/CN103842517A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/13—Dipeptidases (3.4.13)
    • C12Y304/13009—Xaa-Pro dipeptidase (3.4.13.9) i.e. prolidase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/485—Exopeptidases (3.4.11-3.4.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11002—Procollagen-proline dioxygenase (1.14.11.2), i.e. proline-hydroxylase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
CN201280047084.3A 2011-08-03 2012-08-03 胶原蛋白7及相关方法 Pending CN103842517A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711271805.XA CN108165593A (zh) 2011-08-03 2012-08-03 胶原蛋白7及相关方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161514796P 2011-08-03 2011-08-03
US61/514,796 2011-08-03
PCT/US2012/049553 WO2013020064A1 (en) 2011-08-03 2012-08-03 Collagen 7 and related methods

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201711271805.XA Division CN108165593A (zh) 2011-08-03 2012-08-03 胶原蛋白7及相关方法

Publications (1)

Publication Number Publication Date
CN103842517A true CN103842517A (zh) 2014-06-04

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Family Applications (2)

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CN201280047084.3A Pending CN103842517A (zh) 2011-08-03 2012-08-03 胶原蛋白7及相关方法
CN201711271805.XA Pending CN108165593A (zh) 2011-08-03 2012-08-03 胶原蛋白7及相关方法

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Country Status (10)

Country Link
US (1) US9676837B2 (https=)
EP (1) EP2753704A4 (https=)
JP (2) JP2014524241A (https=)
KR (1) KR20140054160A (https=)
CN (2) CN103842517A (https=)
AU (2) AU2012289916B2 (https=)
BR (1) BR112014002546A2 (https=)
CA (1) CA2843896A1 (https=)
MX (1) MX351565B (https=)
WO (1) WO2013020064A1 (https=)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109022464A (zh) * 2018-07-02 2018-12-18 西安巨子生物基因技术股份有限公司 重组人源型胶原蛋白的羟基化方法
CN109069592A (zh) * 2016-03-16 2018-12-21 菲尼克斯组织修复公司 纯化胶原7的方法
CN109536396A (zh) * 2017-09-22 2019-03-29 现代牧场股份有限公司 重组酵母菌株
CN116854807A (zh) * 2023-07-26 2023-10-10 浙江大学 一种重组人源ⅶ型胶原蛋白及其制备方法与应用
CN117143223A (zh) * 2022-08-23 2023-12-01 山西锦波生物医药股份有限公司 一种生物合成人体结构性材料的制备方法
CN120137008A (zh) * 2025-03-18 2025-06-13 广东普言生物科技有限公司 一种重组Ⅶ型胶原蛋白Pro.C7及其制备方法和应用

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6849257B2 (en) 2000-02-04 2005-02-01 Children's Hospital Research Foundation Lipid hydrolysis therapy for atherosclerosis and related diseases
FI3205351T3 (fi) 2010-04-23 2023-07-06 Alexion Pharma Inc Lysosomaalisen kertymätaudin entsyymi
KR20150038636A (ko) 2010-09-09 2015-04-08 시나게바 바이오파르마, 코포레이션 환자에서 리소좀 산 리파제 결핍증의 치료를 위한 리소좀 산 리파제의 용도
WO2012112681A1 (en) 2011-02-15 2012-08-23 Shire Human Genetic Therapies, Inc. Methods for treating lysosomal acid lipase deficiency
US9040484B2 (en) * 2011-04-26 2015-05-26 The Board Of Trustees Of The Leland Stanford Junior University Production and delivery of a stable collagen
EP3099801B1 (en) 2014-01-31 2020-03-18 Factor Bioscience Inc. Synthetic rna for use in the treatment of dystrophic epidermolysis bullosa
AU2015243535A1 (en) 2014-04-08 2016-11-03 University Of Southern California Polypeptide compositions with Type VII collagen fibronectin Type Ill- like repeats and treatment methods for wound closure and healing
KR102204367B1 (ko) * 2014-07-16 2021-01-19 주식회사 엘지생활건강 피부 주름 개선용 화장료 조성물
CA2976376A1 (en) 2015-02-13 2016-08-18 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
GB201601571D0 (en) * 2016-01-28 2016-03-16 Nordic Bioscience As Collagen type Vll alpha 1 assay
IL308824A (en) 2016-08-17 2024-01-01 Factor Bioscience Inc Nucleic acid products and methods of administration thereof
US11180541B2 (en) 2017-09-28 2021-11-23 Geltor, Inc. Recombinant collagen and elastin molecules and uses thereof
CN114401769A (zh) * 2019-03-27 2022-04-26 菲尼克斯组织修复公司 生产胶原蛋白7组合物的系统和方法
EP3953380A4 (en) 2019-04-12 2023-01-25 Geltor, Inc. RECOMBINATION ELASTIN AND ASSOCIATED PRODUCTION
CA3145425A1 (en) 2019-07-03 2021-01-07 Factor Bioscience Inc. Cationic lipids and uses thereof
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
GB2610313B (en) 2020-01-24 2024-07-03 Geltor Inc Animal-free dietary collagen
KR20240004264A (ko) * 2021-03-04 2024-01-11 젤라텍, 인크. 배양된 동물 세포로부터의 콜라겐을 사용한 재료의 제조 방법
MX2024004434A (es) 2021-10-12 2024-07-09 Phoenix Tissue Repair Inc Terapia de reemplazo de proteínas con colágeno 7.

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020142391A1 (en) * 1991-06-12 2002-10-03 Kivirikko Kari I. Synthesis of human procollagens and collagens in recombinant DNA systems
CN1423659A (zh) * 1999-11-12 2003-06-11 法布罗根股份有限公司 重组明胶
CN1552889A (zh) * 2003-12-18 2004-12-08 四川铭让生物科技有限公司 制备胶原蛋白和胶原多肽的方法及其用途

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001009318A1 (fr) * 1999-07-29 2001-02-08 Helix Research Institute Genes associes au cancer du foie
US7026463B2 (en) * 1999-08-13 2006-04-11 Matthew Glenn Polynucleotides and polypeptides, materials incorporating them and methods for using them
JP4490498B2 (ja) 2008-09-30 2010-06-23 新田ゼラチン株式会社 疾病抑制剤
US9040484B2 (en) 2011-04-26 2015-05-26 The Board Of Trustees Of The Leland Stanford Junior University Production and delivery of a stable collagen

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020142391A1 (en) * 1991-06-12 2002-10-03 Kivirikko Kari I. Synthesis of human procollagens and collagens in recombinant DNA systems
CN1423659A (zh) * 1999-11-12 2003-06-11 法布罗根股份有限公司 重组明胶
US20050229264A1 (en) * 1999-11-12 2005-10-13 Chang Robert C Recombinant gelatins
CN1552889A (zh) * 2003-12-18 2004-12-08 四川铭让生物科技有限公司 制备胶原蛋白和胶原多肽的方法及其用途

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHEN M.等: "The Recombinant Expression of Full-length Type VII Collagen and Characterization of Molecular Mechanisms Underlying Dystrophic Epidermolysis Bullosa", 《JOURNAL OF BIOLOGICAL CHEMISTRY》 *
MILTYK W.等: "Prolidase Dependent Inhibition of Collagen Biosynthesis in Chinese Hamster Ovary Cells", 《JOURNAL OF BIOCHEMISTRY》 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109069592A (zh) * 2016-03-16 2018-12-21 菲尼克斯组织修复公司 纯化胶原7的方法
CN109069592B (zh) * 2016-03-16 2023-03-07 菲尼克斯组织修复公司 纯化胶原7的方法
CN109536396A (zh) * 2017-09-22 2019-03-29 现代牧场股份有限公司 重组酵母菌株
CN109022464A (zh) * 2018-07-02 2018-12-18 西安巨子生物基因技术股份有限公司 重组人源型胶原蛋白的羟基化方法
CN117143223A (zh) * 2022-08-23 2023-12-01 山西锦波生物医药股份有限公司 一种生物合成人体结构性材料的制备方法
CN117143223B (zh) * 2022-08-23 2024-03-08 山西锦波生物医药股份有限公司 一种生物合成人体结构性材料的制备方法
CN116854807A (zh) * 2023-07-26 2023-10-10 浙江大学 一种重组人源ⅶ型胶原蛋白及其制备方法与应用
CN120137008A (zh) * 2025-03-18 2025-06-13 广东普言生物科技有限公司 一种重组Ⅶ型胶原蛋白Pro.C7及其制备方法和应用
CN120137008B (zh) * 2025-03-18 2025-09-26 广东普言生物科技有限公司 一种重组Ⅶ型胶原蛋白Pro.C7及其制备方法和应用

Also Published As

Publication number Publication date
EP2753704A1 (en) 2014-07-16
AU2017254910A1 (en) 2017-11-23
JP2017209117A (ja) 2017-11-30
CA2843896A1 (en) 2013-02-07
US20150011733A1 (en) 2015-01-08
MX351565B (es) 2017-10-19
CN108165593A (zh) 2018-06-15
AU2012289916A1 (en) 2014-02-20
BR112014002546A2 (pt) 2017-03-14
MX2014001383A (es) 2014-08-26
KR20140054160A (ko) 2014-05-08
EP2753704A4 (en) 2015-07-08
US9676837B2 (en) 2017-06-13
AU2012289916B2 (en) 2017-08-03
JP2014524241A (ja) 2014-09-22
WO2013020064A1 (en) 2013-02-07

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