CN103804032B - Liquid amino acid compound produced by dead porcine proteins and application thereof - Google Patents
Liquid amino acid compound produced by dead porcine proteins and application thereof Download PDFInfo
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- CN103804032B CN103804032B CN201410042218.3A CN201410042218A CN103804032B CN 103804032 B CN103804032 B CN 103804032B CN 201410042218 A CN201410042218 A CN 201410042218A CN 103804032 B CN103804032 B CN 103804032B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
The invention discloses a liquid amino acid compound produced by dead porcine proteins and an application of the compound. The liquid amino acid compound is obtained by acidifying lean meat by adding sulfuric acid. The liquid amino acid compound produced by acidifying lean meat by adding sulfuric acid is rich in various free amino acids, and the contents of the free amino acids, soluble polypeptide and soluble albuminous total nitrogen are high. The compound diluted as a liquid agricultural amino acid fertilizer leaf fertilizer is sprayed to cucumber, brassica chinensis and radish plants, so that the compound has a very good growth promoting effect. The invention provides a production method of a liquid agricultural amino acid by acidifying lean meat by adding sulfuric acid. The process is simple and the demand on the equipment is low. Compared with a conventional dead pig treatment process, the production method disclosed by the invention has the characteristics that the produced amino acid as the liquid agricultural amino acid fertilizer has the advantages of low treatment cost, and the porcine lean meat resources can be effectively recycled. The compound disclosed by the invention has importance meaning for preserving the ecological environment, effectively solving the problem of treating dead pigs, protecting physical health of people and improving the additional value of agricultural products.
Description
Technical field
The invention belongs to utilization of waste as resource field, relate to a kind of the Liquid amino acid mixture and the application thereof that utilize sick dead pig protein production.
Background technology
1908, Frenchman Lutz made first Amino Acid Absorption sterility test in the world, and demonstrating plant can absorb amino acid.After the 1950's, along with the application of isotopic tracer technique and microautoradiograph technology, plant amino acid nutrition research starts to achieve more deep progress.Perhaps yulan etc. are under sterile culture condition, and with 15N marked glycine and leucine, find separately or mix to use all to make rice seedling dry matter obviously increase, glycine makes rice seedling increase weight 23.7%, and leucine increases weight 30.0%, the two combination treatment weightening finish 41.2%.The inorganic nitrogenous fertilizer of the nitrogen quantity such as Liu Qingcheng etc. spray celery cauline leaf with amino acid mixed solution, and discovery can promote that plant divides grain, and manure trial shows, amino acid whose fertility ratio is high by about 50%.Kinnersley etc. add human glutamic acid and make compound manure in fertilizer, find the validity adding fertilizer, and energy Promoting plant growth, promotes the absorption of Plant To Nutrient, increases the output of plant.In addition, tryptophane, methyllanthionine and methionine(Met) etc. are the precursors of synthesis plant growth hormones ethene and IA A, they may be indirectly to the promoter action of plant-growth, and this amino acid, at Shi Renhou, can produce effect by the level of growth hormone changed in rhizosphere and plant materials.So amino acid has great significance for the growth of plant.
Amino acids industry is a department of infant industry in fermentation industry.Amino acids industry is the most flourishing with Japan, and since nineteen fifty-seven, Japan adopted fermentative Production L-glutamic acid, amino acids industry is developed rapidly, has formed complete amino acids industry system at present, can use fermentative Production 22 seed amino acid.Its Glutamic Acid, methionine(Met) and Methionin form very large market in the world, and increasingly extensive in the application of the departments such as food, medicine, feed, requirement is increasing.Except fermentation method, also has acidolysis at present, the methods such as enzymolysis, the quick grade of such as Sun Zhi with discarded plant protein resource-cottonseed meal and rapeseed meal for raw material, adopt Hydrochloric Acid Hydrolysis Method to prepare aminoacids complex, not only obtain amino acid, also to a certain degree reduce the toxicity of cottonseed meal and rapeseed meal.Mu Jun etc. utilize microwave method acidolysis to discard animal toenail to prepare aminoacids complex, and microwave method has good promoter action to animal toenail acidolysis, compares can shorten hydrolysis time with conventional acid hydrolysis method, reduce sulfuric acid dosage, greatly reduce production cost.Cheng Jiahao etc. to pancreatin be catalyzer hydrolysis silkworm chrysalis prepare kilnitamin, the features such as it is high that enzymolysis process has efficiency, and amino acid yield is high, reaction conditions is gentle.
Along with the exposure of a large amount of floating dead pig event in section waters, Shanghai Huangpu River Songjiang, people have recognized that sick dead pig brings serious harm to human lives and production.The method of current China process sick dead pig roughly has corpse burning method, pyroprocessing method and bury method, but corpse burning method and pyroprocessing method all need to consume a large amount of energy, the method of burying can cause secondary pollution, and such treatment process does not all have a large amount of protein-based resource contained by the sick dead pig of efficient resource utilization.
For above situation, this patent research adopts sulfuric acid solution method that sick dead pig acidolysis is become Liquid amino acid, and has carried out utilizing it to promote the research of crop quality aspect as Foliar Spraying crop, thus reaches object sick dead pig turned waste into wealth.
Summary of the invention
The present invention is directed to that the harm of current sick dead pig is serious, processing cost large, and can not get the situation of efficient resource, develop a kind of Liquid amino acid mixture utilizing sick dead pig protein production.
Another object of the present invention is to provide the production method of this Liquid amino acid mixture.
Another object of the present invention is to provide the application of this Liquid amino acid mixture.
Object of the present invention realizes by following technical scheme:
A kind of Liquid amino acid mixture adds sulfuric acid solution by lean meat to form.
Wherein, described lean meat is preferably from the lean meat of pig, ox, sheep; Preferred lean pork, further preferably derives from the lean pork of sick dead pig further.
The sulfuric acid of described acidolysis to be working concentration be 5 ~ 7mol/L is to lean meat hydrolysis 5 ~ 7h at 80 ~ 90 DEG C, and the material ratio of the sulfuric acid of lean meat and 5 described ~ 7mol/L (mass volume ratio, lower together) is 1:1.5 ~ 2.5; Preferred working concentration is that the sulfuric acid of 5mol/L is hydrolyzed 6h to lean meat at 80 DEG C, and the material ratio of the sulfuric acid of lean meat and described 5mol/L is 1:1.5.
The sulfuric acid that the present invention uses derives from the technical grade vitriol oil that market is bought, and sulfuric acid content >=98%, is adjusted to 5 ~ 7mol/L during use.
The production method of Liquid amino acid mixture of the present invention, uses sulfuric acid to carry out acidolysis to lean meat.
Wherein, described lean meat is preferably from the lean meat of pig, ox, sheep; Preferred lean pork, further preferably derives from the lean pork of sick dead pig further.
The sulfuric acid of described acidolysis to be working concentration be 5 ~ 7mol/L is to lean meat hydrolysis 5 ~ 7h at 80 ~ 90 DEG C, and the material ratio of the sulfuric acid of lean meat and 5 described ~ 7mol/L (mass volume ratio, lower together) is 1:1.5 ~ 2.5; Preferred working concentration is that the sulfuric acid of 5mol/L is hydrolyzed 6h to lean meat at 80 DEG C, and the material ratio of the sulfuric acid of lean meat and described 5mol/L is 1:1.5.
Liquid amino acid mixture of the present invention is as the application of liquid agricultural amino acid fertilizer.
Beneficial effect
The Liquid amino acid mixture that sulfuric acid solution lean pork of the present invention is produced contains total free aminoacids more than 34.00g/L(fresh weight), trip state nitrogen is more than 0.80g/L(fresh weight), total free aminoacids, soluble polypeptide and soluble proteins state total nitrogen are more than 10.00g/L(fresh weight).Dilution, as liquid agricultural amino acid fertilizer Foliar Spraying cucumber, Xiaoqinling Nature Reserve and radish plant, possesses good growth-promoting effect.Simultaneously because sick dead pig belongs to cost free raw material, produce amino acid cost significantly lower than current Liquid amino acid product.
The invention provides a kind of sulfuric acid solution lean pork and produce the amino acid whose production method of agricultural liquid.Technique is simple, and equipment requirements is low, and the amino acid of production, as liquid agricultural amino acid fertilizer, is compared conventional sick dead pig treatment technology and had the low advantage of processing cost, and can effective recycling lean pork resource.Invention is simultaneously for preserving the ecological environment, and the problem of effective process sick dead pig, the protection people's is healthy, and the added value improving agricultural-food has great importance.
Accompanying drawing explanation
The impact that Fig. 1 sulfuric acid concentration is degraded on lean pork
The impact that Fig. 2 acidolysis temperature is degraded on lean pork
The impact that Fig. 3 material ratio is degraded on lean pork
The impact that Fig. 4 acidolysis time degrades on lean pork
Common 17 seed amino acids of Fig. 5 acid hydrolysis solution measure
A: the amino acidanalyser collection of illustrative plates of 16 kinds of common amino acids except proline(Pro);
B: amino proline acid assay instrument collection of illustrative plates
Embodiment
Embodiment 1
1, the acquisition of lean pork
Thered is provided by certain pig farm.
2, the research of acidolysis condition
1) sulfuric acid concentration: set different sulfuric acid concentration (3mol/L, 4mol/L, 5mol/L and 6mol/L) acidolysis under 80 DEG C of conditions, material ratio 1:2(1g lean pork: 2mL sulfuric acid), acidolysis time 5h, filtered through gauze, dries the degradation rate measuring lean pork for 50 DEG C.
Result: as shown in Figure 1, acid concentration is higher than 5mol/L, and the degradation rate there was no significant difference of lean pork, the sulfuric acid concentration of 5mol/L is defined as optimum interpolation acid concentration.
2) acidolysis temperature: set different temperature (60 DEG C, 70 DEG C, 80 DEG C and 90 DEG C) at sulfuric acid concentration 5mol/L, material ratio 1:2(1g lean pork: 2mL sulfuric acid) and acidolysis time 5h condition under acidolysis, filtered through gauze, dries the degradation rate measuring lean pork for 50 DEG C.
Result: as shown in Figure 2, during the situation of temperature higher than 80 DEG C, the degradation rate there was no significant difference of lean pork, 80 DEG C are finally defined as optimum acidolysis temperature.
3) material ratio: set different material ratio (1:1,1:1.5,1:2 and 1; 2.5), at sulfuric acid concentration 5mol/L, acidolysis under temperature 80 DEG C and acidolysis time 5h condition, filtered through gauze, dries the degradation rate measuring lean pork for 50 DEG C.
Result: as shown in Figure 3, when material ratio is higher than 1:1.5, the degradation rate there was no significant difference of lean pork, 1:1.5 finally confirms as optimum material proportion.
4) the acidolysis time: set the different acidolysis time (3h, 4h, 5h and 6h), at sulfuric acid concentration 5mol/L, acidolysis under temperature 80 DEG C and material ratio 1:1.5 condition, filtered through gauze, dries the degradation rate measuring lean pork for 50 DEG C.
Result: as shown in Figure 4, acidolysis 6 hours, lean pork can effectively be degraded, and within 6 hours, finally confirms as the optimum acidolysis time.
More than comprehensive, the processing condition of best acidolysis lean pork are: sulfuric acid concentration 5mol/L, temperature 80 DEG C, material ratio 1:1.5, time 6h.The palliating degradation degree of lean pork is 99.63%.
3. the research of acid hydrolysis solution composition
1) acid hydrolysis solution sodium citrate buffer solution (amino acidanalyser sample-loading buffer) is diluted 10 times, sample introduction 20ul measures in amino acidanalyser (model Biochrom30).As shown in Figure 5, acid hydrolysis solution is containing common 16 kinds of total free aminoacidss, and calculating total free amino acid content by standard model is 34.73g/L(fresh weight for result).
2) acid hydrolysis solution pure water is diluted 100 times, it is 0.80g/L(fresh weight that BRAN+LUEBBE AA3 model Flow Analyzer measures free ammonium nitrogen content in acid hydrolysis solution);
3) get after former acid hydrolysis solution cleared up by the vitriol oil and strong hydrogen peroxide solution, digestion solution ammonium nitrogen is measured by BRAN+LUEBBE AA3 model Flow Analyzer, calculating acid hydrolysis solution total nitrogen is 10.33g/L(fresh weight), total nitrogen is deducted former acid hydrolysis solution to dissociate ammonium nitrogen, draw former acid hydrolysis solution total free aminoacids, soluble polypeptide and soluble proteins state total nitrogen 9.53g/L(fresh weight).
4, greenhouse test
1) growth-promoting test is all carried out in national organic fertilizer Engineering Technical Research Centre (Jiangsu) intelligent greenhouse, and first time test establishes 1 contrast, 12 process, each process 9 repetition altogether.The pork acid hydrolysis solution that process is respectively this patent development dilute 100 times (Z100), 500 times (Z500), 1000 times (Z1000), 2000 times (Z2000), 4000 times (Z4000) and 6000 times (Z6000) and this patent development pork acid hydrolysis solution adjust through ammoniacal liquor to dilute 100 times of (ZT100), 500 times (ZT500) after pH to 6.0,1000 times (ZT1000), 2000 times (ZT2000), 4000 times (ZT4000) and 6000 times (ZT6000) spray cucumber seedling liquid level, contrast as clear water process.Crop is cucumber, and mountain rugged cucumber formula nutritional liquid (200ml) water planting is cultivated, and within every 3 days, changes one time of nutrition liquid, and sprays the acid hydrolysis solution of this research development, after spraying 4 times, measures growth index after 12 days.Result as shown in Table 1 and Table 2, plant height: as can be seen from Table 1, Z4000 process is significantly better than other process, and Z100 process extension rate is too little, have certain restraining effect to crop plant height, the process of ZT extension rate process relative Z extension rate does not significantly improve effect.Stem is thick: as can be seen from Table 1, except ZT2000 process, the stem of Z4000 process is slightly significantly better than other process, and Z100 process extension rate is too little, slightly have certain restraining effect to stem of plant, the process of ZT extension rate does not significantly improve effect relative to the same extension rate process of Z.Three leaves that fall are long: as can be seen from Table 1, Z4000 process is comparatively except ZT2000, beyond Z2000, other process all have significant difference, Z100 process extension rate is too little, crop is fallen to three leaves are long certain restraining effect, the process of ZT extension rate does not significantly improve effect relative to the same extension rate process of Z.Three leaves that fall are wide: as can be seen from Table 1, Z4000 process is CK significant difference comparatively, and compared with other process, but the wide maximum difference of leaf is not remarkable.Z100 process extension rate is too little, crop is fallen to three leaves are wide certain restraining effect, and the process of ZT extension rate does not significantly improve effect relative to the same extension rate process of Z.Chlorophyll: as can be seen from Table 1, Z4000 manages comparatively CK significant difference, and compared with other process, but the maximum difference of chlorophyll measuring value is not remarkable.Z100 process extension rate is too little, has certain restraining effect to crop chlorophyll, and the relative Z of ZT extension rate process does not significantly improve effect with extension rate process.The number of blade: as can be seen from Table 2, Z4000 manages comparatively CK significant difference, and compared with other process, the number of blade is maximum.Z100 process extension rate is too little, and have certain restraining effect to crop leaf growth, the process of ZT extension rate does not significantly improve effect relative to the same extension rate process of Z.Overground part fresh weight: as can be seen from Table 2, Z4000 process comparatively beyond ZT4000 other process all there is significant difference, Z100 process extension rate is too little, have certain restraining effect to crop aerial growth, the process of ZT extension rate does not significantly improve effect relative to the same extension rate process of Z.Overground part dry weight: as can be seen from Table 2, Z4000 process comparatively beyond ZT4000 other process all there is significant difference, Z100 process extension rate is too little, have certain restraining effect to crop aerial growth, the process of ZT extension rate does not significantly improve effect relative to the same extension rate process of Z.Underground part fresh weight: as can be seen from Table 2, Z4000 process comparatively other process all has significant difference, Z100 process extension rate is too little, and have certain restraining effect to the part of a crop which is beneath the ground growth, the process of ZT extension rate does not significantly improve effect relative to the same extension rate process of Z.Underground part dry weight: as can be seen from Table 2, Z4000 process comparatively other process all has significant difference, Z100 process extension rate is too little, and have certain restraining effect to the part of a crop which is beneath the ground growth, the process of ZT extension rate does not significantly improve effect relative to the same extension rate process of Z.To sum up analyze, Z4000 process has good growth-promoting functions to cucumber growth, and acid hydrolysis solution does not need to regulate pH directly can dilute use, compared to clear water contrast, dilute 4000 times and sprays, significantly improve the plant height of plant, stem is thick, three leaves that fall are long, three leaves wide, chlorophyll, the number of blade, overground part fresh weight, overground part dry weight, underground part fresh weight and underground part dry weight, improve 46% respectively, 18%, 31%, 24%, 21%, 15%, 61%, 76%, 266% and 124%.
2) test of second time growth-promoting establishes 1 contrast, 3 process, each process 6 repetition altogether.The pork acid hydrolysis solution that process is respectively this patent development dilutes 2000 times (Z2000), 4000 times (Z4000) and 6000 times (Z6000), contrasts as clear water process.Crop is Xiaoqinling Nature Reserve, plants and (seedling nutritious soil that market is bought is housed) in seedling pan, within every 3 days, spray the acid hydrolysis solution of this research development, spray 4 times (12 days) and measure growth index afterwards.As shown in Table 3 and Table 4, plant height, the stem for the treatment of group are thick, three leaves that fall are long and three leaves are wide all high than CK for result, and Z4000 process comparatively Ck significant difference.Chlorophyll: as can be seen from Table 3, treatment group and CK indifference remarkable.As can be seen from Table 4, the number of blade for the treatment of group, overground part fresh weight, overground part dry weight, underground part fresh weight and underground part dry weight are all high than CK, and Z4000 process comparatively Ck significant difference.To sum up analyze, Z4000 process significantly improves the plant height of plant, stem is thick, three leaves that fall are long, fall that three leaves are wide, the number of blade, overground part fresh weight, overground part dry weight, underground part fresh weight and underground part dry weight, improve 34% respectively, 39%, 24%, 79%, 24%, 127%, 144%, 141% and 220%, to Xiaoqinling Nature Reserve growth, there is good growth-promoting functions.
3) third time establishes 1 contrast, 3 process, each process 6 repetition equally altogether.The pork acid hydrolysis solution that process is respectively this patent development dilutes 2000 times (Z2000), 4000 times (Z4000) and 6000 times (Z6000), contrasts as clear water process.Crop is radish, plants and (Nutrition Soil that market is bought is housed) in seedling pan, within every 3 days, spray the acid hydrolysis solution of this research development, spray 4 times (12 days) and measure growth index afterwards.As shown in table 5 and table 6, as can be seen from Table 5, plant height, the stem for the treatment of group are thick, three leaves that fall are long, three leaves are wide, chlorophyll is all high than CK process for result, and Z4000 process comparatively Ck significant difference.As can be seen from table six, the number of blade for the treatment of group, overground part fresh weight, overground part dry weight, underground part fresh weight and underground part dry weight are all high than CK, and Z4000 process comparatively Ck significant difference.To sum up analyze, Z4000 process significantly improves the plant height of plant, stem is thick, three leaves that fall are long, fall that three leaves are wide, chlorophyll, the number of blade, overground part fresh weight, overground part dry weight, underground part fresh weight and underground part dry weight, improves 14% respectively, 66%, 26%, 40%, 12%, 29%, 51%, 53%, 47% and 70%, to radish growth, there is good growth-promoting functions.
Table 1 different treatment is on the impact of cucumber growth
Table 2 different treatment is on the impact of cucumber growth
The impact that table 3 different treatment grows Xiaoqinling Nature Reserve
The impact that table 4 different treatment grows Xiaoqinling Nature Reserve
Table 5 different treatment is on the impact of radish growth
Table 6 different treatment is on the impact of radish growth
Claims (1)
1. a Liquid amino acid mixture, it is characterized in that adding sulfuric acid solution by the lean meat of sick dead pig forms; The sulfuric acid of described acidolysis to be working concentration be 5 ~ 7mol/L is to lean meat hydrolysis 5 ~ 7h at 80 ~ 90 DEG C, and the material ratio of the sulfuric acid of lean meat and 5 described ~ 7mol/L is 1:1.5 ~ 2.5.
2, Liquid amino acid mixture according to claim 1, it is characterized in that working concentration be the sulfuric acid of 5mol/L at 80 DEG C to lean meat hydrolysis 6h, the material ratio of the sulfuric acid of lean meat and described 5mol/L is 1:1.5.
3, the production method of Liquid amino acid mixture according to claim 1, it is characterized in that working concentration is the sulfuric acid of the 5 ~ 7mol/L lean meat hydrolysis 5 ~ 7h to sick dead pig at 80 ~ 90 DEG C, the material ratio of the sulfuric acid of lean meat and 5 described ~ 7mol/L is 1:1.5 ~ 2.5.
4, production method according to claim 3, is characterized in that; Working concentration is that the sulfuric acid of 5mol/L is hydrolyzed 6h to lean meat at 80 DEG C, and the material ratio of the sulfuric acid of lean meat and described 5mol/L is 1:1.5.
5, Liquid amino acid mixture according to claim 1 is directly as the application of liquid agricultural amino acid fertilizer.
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| CN104190694B (en) * | 2014-08-26 | 2015-11-25 | 南京农业大学 | The no pollution harmless treatment of dead livestock and poultry animal and high added value resource utilization process |
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| CN107377578A (en) * | 2017-06-22 | 2017-11-24 | 烟台大学 | A kind of dead livestock and poultry animal method for innocent treatment |
| CN111269064A (en) * | 2018-12-03 | 2020-06-12 | 江苏北斗星环保股份有限公司 | Bio-based organic fertilizer for livestock and poultry died of diseases and preparation method thereof |
| CN111375618B (en) * | 2018-12-27 | 2022-04-22 | 江苏北斗星环保股份有限公司 | Harmless amino acid-containing aqueous solution for livestock and poultry died of diseases and preparation method thereof |
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| CN112280712A (en) * | 2020-11-02 | 2021-01-29 | 南京农业大学 | High-density liquid fermentation medium for bacteria and fermentation method thereof |
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Application publication date: 20140521 Assignee: Gansu Silk Road Shengfeng biological science and Technology Group Co., Ltd. Assignor: Nanjing Agricultural University Contract record no.: 2017320000212 Denomination of invention: Liquid amino acid compound produced by dead porcine proteins and application thereof Granted publication date: 20150527 License type: Common License Record date: 20171227 |