CN103800268A - Enteromorpha crude polysaccharide cream and preparation method thereof - Google Patents
Enteromorpha crude polysaccharide cream and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the field of cosmetics, and in particular relates to a crude enteromorpha polysaccharide cream and a preparation method thereof. The crude enteromorpha polysaccharide cream comprises the following raw materials in percentage by weight: 2.5-5 percent of crude enteromorpha polysaccharide, 4-6 percent of a humectant, 0.05-0.1 percent of a preservative, 0.5-1.5 percent of a nutrition additive, 5-7.5 percent of an oily raw material, 1-2 percent of a thickening agent, 0.05-0.1 percent of an antisensitive agent, 0.5-1 percent of a freshener, 0.5-1.5 percent of a solubilizer, and the balance of deionized water. Tests prove that the total oxidation resistance of the cream is over 40mM/g, after the cream is continuously used for 6 days, the skin moisture content is increased by over 60 percent, the skin collagen fiber content is increased by over 120 percent, and the skin elasticity is improved by over 230 percent. The crude enteromorpha polysaccharide cream is a skin-care product with remarkable beautifying effect and low price.
Description
Technical field
The invention belongs to cosmetic field, particularly a kind of Entermorpha crude polysaccharides protective skin cream and preparation method thereof.
Background technology
Protective skin cream is a kind of facial cosmetics and skincare product that spreads on, and is to form natural sebum film at the epidermis of skin, keeps the skin wet and nutritional labeling to skin, can repair the damaged cell of epiderm skin simultaneously, improves elasticity, pliability and the brightness of skin.The kind of protective skin cream is a lot, and wherein Sargassum crude polysaccharides protective skin cream adds Sargassum crude polysaccharides composition, and the effects such as moisturizing, antioxidation, radioprotective can be provided, and makes skin remain fine and smooth glossy.
Entermorpha is a kind of common large ocean chlorella, and it belongs to Chlorophyta, Chlorophyceae, Ulvales, Ulvaceae in Sargassum.The crude polysaccharides of Entermorpha has the pharmacological actions such as the cholesterol of reduction, antibacterial and antiviral, antioxidation, enhancing immunologic function, antitumor, antiinflammatory.And Entermorpha also has multiple biological activity, in cosmetic applications, main manifestations is antioxidation, radioprotective, antiviral, the aspects such as moisture retention.In recent years, along with progressively going deep into that its chemical composition is studied, its medical mechanism is also illustrated gradually, and the step of drug development is constantly accelerated.
Utilize Entermorpha crude polysaccharides to prepare protective skin cream, in existing document and report, record not yet to some extent.If people can constantly research and develop effect of Entermorpha crude polysaccharides, utilize the characteristic of its antioxidation, radioprotective, moisture retention etc., be developed to there is antioxidation, the protective skin cream of moisturizing and the anti-ageing effect of waiting for a long time, opened up the new application of Entermorpha.
Summary of the invention
The object of this invention is to provide a kind of Entermorpha crude polysaccharides protective skin cream, this protective skin cream can improve skin non-oxidizability, and obviously Glycerin, increases skin elasticity, slows down skin aging.
Another object of the present invention is to provide a kind of preparation method of above-mentioned Entermorpha crude polysaccharides protective skin cream.
Object of the present invention can be achieved through the following technical solutions:
A kind of Entermorpha crude polysaccharides protective skin cream, is characterized in that, comprises following percentage by weight and count the raw material of meter:
Entermorpha crude polysaccharides 2.5-5%, wetting agent 4-6%, antiseptic 0.05-0.1%, nourishing additive agent 0.5-1.5%, oiliness raw material 5-7.5%, thickening agent 1-2%, anti-allergic agent 0.05-0.1%, freshener 0.5-1%, solubilizing agent 0.5-1.5%, surplus is deionized water.
Above-mentioned Entermorpha crude polysaccharides is prepared by the following method,
Entermorpha is immersed in to 12-24 hour in the ethanol that mass concentration is 90%-95%, filters, dry; The Entermorpha of drying and distilled water are soaked to 2-4 hour according to the proportioning of 1g:25-45mL, smash rear 95 ℃ of heated at constant temperature 10-20 minute to pieces, cooling, cross leaching filtrate, after filtrate heating concentrated (filtrate is concentrated into 95% water content), supernatant is got in centrifugalize; After ethanol precipitate with ethanol 24-28h, filter, taking precipitate lyophilizing, obtains Entermorpha crude polysaccharides.Preferably, described Entermorpha is the one in bar Entermorpha, edge pipe Entermorpha or bent Entermorpha.
Described wetting agent is one or more the mixture in glycerol, amino acid humectant, propylene glycol, butanediol.Preferably, described wetting agent is the mixture of glycerol, amino acid humectant, propylene glycol, butanediol, and wherein, the weight ratio of glycerol, amino acid humectant, propylene glycol, butanediol is 2-4:1:2-4:2-4.
Described antiseptic is one or both the mixture in phenoxyethanol or Sang Pu K15; Preferably, described antiseptic is the mixture of phenoxyethanol and Sang Pu K15, and wherein, the weight ratio of phenoxyethanol and Sang Pu K15 is 1-2:1.
Described anti-allergic agent is one or both the mixture in water solublity a-bisabolol or glycyrrhizic acid dipotassium.Preferably, described anti-allergic agent is the mixture in water solublity a-bisabolol and glycyrrhizic acid dipotassium, and wherein, the weight ratio of water solublity a-bisabolol and glycyrrhizic acid dipotassium is 1-2:1.
Described nourishing additive agent is one or more the mixture in collagen protein powder, ceramide, silk fibroin powder and Limax mucus extract.Preferably, described nourishing additive agent is the mixture of collagen protein powder, ceramide and Limax mucus extract, and wherein, the weight ratio of described collagen protein powder, ceramide and Limax mucus extract is 1-2:1-2:1.
Described oiliness raw material is one or both the mixture in Jojoba oil, isopropyl myristate (IPM).Preferably, described oiliness raw material is the mixture of Jojoba oil, IPM, wherein, Jojoba oil, with the weight ratio of IPM be 2-3:1.
Described thickening agent is one or both mixture of high molecular cellulose or HANSHENGJIAO.Preferably, described thickening agent is the mixture of high molecular cellulose and HANSHENGJIAO, and wherein, the weight ratio of high molecular cellulose and HANSHENGJIAO is 1-2:1.
Described freshener is Fructus Citri Limoniae quintessence oil, Flos Rosae Rugosae quintessence oil, Garden lavender quintessence oil, Bergamot, rosemary ethereal oil, the mixture of one or more in Flos Pelargonii quintessence oil.
Described solubilizing agent is quintessence oil emulsifying agent.
The preparation method of above-mentioned Entermorpha crude polysaccharides protective skin cream, its step comprises:
(1), Entermorpha is immersed in to 12-24 hour in the ethanol that mass concentration is 90%-95%, filtration, oven dry; The Entermorpha of drying and distilled water are soaked to 2-4 hour according to the proportioning of 1g:25-45mL, smash rear 95 ℃ of heated at constant temperature 10-20 minute to pieces, cooling, cross leaching filtrate, after filtrate heating is concentrated, supernatant is got in centrifugalize; After ethanol precipitate with ethanol 24-28h, filter, taking precipitate lyophilizing, obtains Entermorpha crude polysaccharides; Preferably, described Entermorpha is the one in bar Entermorpha, edge pipe Entermorpha or bent Entermorpha.
(2), weigh good various raw materials according to formula;
(3), Entermorpha crude polysaccharides is added in deionized water constantly and stirs and make it swelling, add at glycerol post-heating to 70 ℃-90 ℃ of temperature and dissolve; Oiliness raw material is heating for dissolving under 70 ℃ of-90 ℃ of conditions, then both is mixed, and adds solubilizing agent, and emulsifying is stirred; Emulsion is cooled to 50-60 ℃, stirs after adding wetting agent, antioxidant, antiseptic, anti-allergic agent, nourishing additive agent; Emulsion is cooled to 40-45 ℃, adds tackifier, freshener to stir; The weight ratio of described Entermorpha crude polysaccharides and glycerol is 1-3:1.
In described step (1), in ethanol precipitate with ethanol, the mass concentration of alcoholic solution used is 75-80%.
Compared with prior art, beneficial effect of the present invention is:
1, described Entermorpha crude polysaccharides protective skin cream can obviously improve skin antioxidation, moisturizing and defying age ability, and wherein, the total antioxidant capacity of Entermorpha crude polysaccharides protective skin cream is more than 40mM/g; After using six days continuously, skin moisture content increases more than 60%, and skin collagen fiber content increases more than 120%, and skin elasticity improves more than 230%.
2, the preparation method of described Entermorpha crude polysaccharides protective skin cream is simple, easy to operate, and raw material is easy to get, cost is low, low price, and the medicine adding in preparation process does not only injure skin, and environmentally safe, green, environmental protection.
Accompanying drawing explanation
Fig. 1 is the FRAP method standard curve in embodiment 1.
Fig. 2 is the Entermorpha crude polysaccharides emollient cream water content detection result in embodiment 1.
Fig. 3 is the Entermorpha crude polysaccharides emollient cream collagen fabric testing result in embodiment 1.
Fig. 4 is the Entermorpha crude polysaccharides emollient cream elastomeric check result in embodiment 1.
The specific embodiment
Below in conjunction with embodiment, the invention will be further described:
(1) preparation of Entermorpha crude polysaccharides
By the fresh edge pipe gathering alcoholic solution (mass concentration is 95%) immersion filtration after 12 hours, oven dry for Entermorpha, Entermorpha after oven dry adds in distilled water, wherein, the amount ratio of Entermorpha and distilled water is 1g:40mL, to soak after the Entermorpha tissue mashing of 2 hours at 95 ℃ heated at constant temperature 15 minutes, cooled and filtered; Supernatant is got in the rear centrifugalize of filtrate concentrated (filtrate is concentrated into 95% water content) under 70 ℃ of heating, with filtering after alcoholic solution (mass concentration is 80%) precipitate with ethanol 24h, obtains Entermorpha crude polysaccharides after taking precipitate lyophilizing.
(2) preparation of protective skin cream
A, weigh (percentage by weight) according to following formula:
Entermorpha crude polysaccharides: 2.5%;
Wetting agent: 5%(wherein, glycerol: 1.5%, amino acid humectant: 0.5%, propylene glycol 1.5%, butanediol: 1.5%);
Antiseptic: 0.06%(wherein, phenoxyethanol: 0.03%, Sang Pu K15:0.03%);
Anti-allergic agent: 0.06%(wherein, water solublity a-bisabolol: 0.03%, glycyrrhizic acid dipotassium: 0.03%);
Nourishing additive agent: 1.5%(wherein, collagen protein powder: 0.5%, ceramide: 0.5%, Limax mucus extract: 0.5%);
Oiliness raw material: 7.5%(wherein, Jojoba oil: 5%, IPM:2.5%);
Thickening agent: 1.25%(wherein, high molecular cellulose: 0.75%, HANSHENGJIAO: 0.5%);
Flos Rosae Rugosae quintessence oil: 0.7%;
Quintessence oil emulsifying agent: 1%;
Surplus is deionized water.
B, Entermorpha crude polysaccharides is joined in deionized water constantly and stirs and make it swelling, and then add glycerol, wherein, the weight ratio of Entermorpha crude polysaccharides and glycerol is 5:3, is heated to 70 ℃ of dissolvings; Oiliness raw material, 70 ℃ of heating for dissolving, then mixes both, add quintessence oil emulsifying agent, and emulsifying is stirred; Emulsion is cooled to 50-60 ℃, stirs 3 minutes after adding wetting agent, antioxidant, antiseptic, anti-allergic agent, nourishing additive agent; Emulsion is cooled to 40-45 ℃, adds tackifier, Flos Rosae Rugosae quintessence oil to stir.
(3) testing result
A, total antioxidation test
Measure total antioxidant capacity by FRAP method, testing procedure is as follows:
By specification requires configuration FRAP working solution, hatches for 37 ℃.Configuration 100mmol/L FeSO4 standard solution, and be diluted with distilled water into 0.15,0.3,0.6,0.9,1.2 and 1.5mmol/L.Take the Entermorpha crude polysaccharides protective skin cream 0.015g making in above-mentioned steps (2), add 0.3mL distilled water diluting and dissolve.In each detection hole of 96 orifice plates, add 180 microlitre FRAP working solutions.In blank hole, add 5 microlitre distilled water; Standard curve detects in hole and adds respectively 5 microlitres, and concentration is 0.15,0.3,0.6,0.9,1.2 and the FeSO4 standard solution of 1.5mmol/L concentration, establishes 3 repetitions; In sample detection hole, add 5 microlitre Entermorpha crude polysaccharides protective skin cream samples, establish 2 repetitions; Mix gently.Hatch after 3-5 minute for 37 ℃ and record the average OD value of standard solution and Entermorpha crude polysaccharides protective skin cream at 595nm place, obtain standard curve: y=1.221x+0.042, specifically as shown in Figure 1.
The absorbance that calculates 1mmol/L FeSO4 standard solution according to standard curve is 1.263, Entermorpha crude polysaccharides protective skin cream is average OD value 0.762 at 595nm place, total antioxidant capacity is: (0.762/1.263) mM/0.015g=40.22mM/g, the larger oxidation resistance of this numerical value is stronger.
B, moisturizing and defying age performance test
Before test, experimenter's arm of cleaning inner side, and with wet gauze (with deionized water) scrub, water temperature is room temperature, after cleaning, carry out measurement markers in experimenter's both hands forearm inner side, each test zone interval 1cm, every place pilot region is 4cm*4cm, within before tested position 2-3 days, can not use any cosmetic product; Experimenter sits quietly after 30min in room temperature environment, use capacitance method moisture of skin analyzer (SG-5D) to measure the moisture value in each region, with 2,000,000 pixel CCD50X portable high-definition intelligent skin detection instrument (EH-800U) mensuration skin collagen fiber content and elasticity indexes.Afterwards test products is evenly applied in arm trial zone, carries out measurement and the observation in tested region after 40min, each region parallel assay five times according to the order of sequence, draws meansigma methods.Tested region METHOD FOR CONTINUOUS DETERMINATION six days, measure and observe every day after product is smeared 40min.
Result shows that use is continuously after six days, and moisture increases by 63%, and specifically as shown in Figure 2, collagen fabric content increases by 126%, and specifically as shown in Figure 3, elasticity improves 239%, specifically as shown in Figure 4.
Testing result shows that antioxidation, moisturizing and the defying age of the Entermorpha crude polysaccharides protective skin cream of preparing in the present embodiment is very capable.
(1) preparation of Entermorpha crude polysaccharides is with embodiment 1.
(2) preparation of protective skin cream
A, weigh (percentage by weight) according to following formula:
Entermorpha crude polysaccharides: 3%;
Wetting agent: 6%(wherein, glycerol: 2%, amino acid humectant: 0.75%, propylene glycol 1.75%, butanediol: 1.5%);
Antiseptic: 0.05%(wherein, phenoxyethanol: 0.03%, Sang Pu K15:0.02%);
Anti-allergic agent: 0.06%(wherein, water solublity a-bisabolol: 0.03%, glycyrrhizic acid dipotassium: 0.03%);
Nourishing additive agent: 1.25%(wherein, collagen protein powder: 0.5%, ceramide: 0.5%, Limax mucus extract: 0.25%);
Oiliness raw material: 7%(wherein, Jojoba oil: 5%, IPM:2%);
Thickening agent: 1.5%(wherein, high molecular cellulose: 0.75%, HANSHENGJIAO: 0.75%);
Garden lavender quintessence oil: 0.7%;
Quintessence oil emulsifying agent: 1%;
Surplus is deionized water.
B, Entermorpha crude polysaccharides is joined in deionized water constantly and stirs and make it swelling, and then add glycerol, wherein, the weight ratio of Entermorpha crude polysaccharides and glycerol is 5:3, is heated to 70 ℃ of dissolvings; Oiliness raw material, 70 ℃ of heating for dissolving, then mixes both, add quintessence oil emulsifying agent, and emulsifying is stirred; Emulsion is cooled to 50-60 ℃, stirs 3 minutes after adding wetting agent, antioxidant, antiseptic, anti-allergic agent, nourishing additive agent; Emulsion is cooled to 40-45 ℃, adds tackifier, Garden lavender quintessence oil.
(3) testing result
A, total antioxidation method of testing are with embodiment 1, and total antioxidant capacity is 41.05mM/g.
B, moisturizing and defying age performance test methods be with embodiment 1,
Result shows that use is continuously after six days, and moisture increases by 74%, and collagen fabric content increases by 124%, and elasticity improves 237%.
The above, show according to testing result eventually, and antioxidation, moisturizing and the defying age of the Entermorpha crude polysaccharides protective skin cream of preparing in the present invention are very capable, are a kind of low price, the significant skin care item of cosmetic result.
The above is preferred embodiment of the present invention, but the present invention should not be confined to the disclosed content of this embodiment.Do not depart from the equivalence completing under spirit disclosed in this invention so every or revise, all falling into the scope of protection of the invention.
Claims (10)
1. an Entermorpha crude polysaccharides protective skin cream, is characterized in that, comprises following percentage by weight and count the raw material of meter:
Entermorpha crude polysaccharides 2.5-5%, wetting agent 4-6%, antiseptic 0.05-0.1%, nourishing additive agent 0.5-1.5%, oiliness raw material 5-7.5%, thickening agent 1-2%, anti-allergic agent 0.05-0.1%, freshener 0.5-1%, solubilizing agent 0.5-1.5%, surplus is deionized water.
2. Entermorpha crude polysaccharides protective skin cream according to claim 1, is characterized in that: described Entermorpha crude polysaccharides is prepared by the following method,
Entermorpha is immersed in to 12-24 hour in the ethanol that mass concentration is 90%-95%, filters, dry; The Entermorpha of drying and distilled water are soaked to 2-4 hour according to the proportioning of 1g:25-45mL, smash rear 95 ℃ of heated at constant temperature 10-20 minute to pieces, cooling, cross leaching filtrate, after filtrate heating is concentrated, supernatant is got in centrifugalize; After ethanol precipitate with ethanol 24-28h, filter, taking precipitate lyophilizing, obtains Entermorpha crude polysaccharides.
3. Entermorpha crude polysaccharides protective skin cream according to claim 1, is characterized in that: described wetting agent is one or more the mixture in glycerol, amino acid humectant, propylene glycol, butanediol.
4. Entermorpha crude polysaccharides protective skin cream according to claim 1, is characterized in that: described antiseptic is one or both the mixture in phenoxyethanol or Sang Pu K15; Described anti-allergic agent is one or both the mixture in water solublity a-bisabolol or glycyrrhizic acid dipotassium.
5. Entermorpha crude polysaccharides protective skin cream according to claim 1, is characterized in that: described nourishing additive agent is one or more the mixture in collagen protein powder, ceramide and Limax mucus extract.
6. Entermorpha crude polysaccharides protective skin cream according to claim 1, is characterized in that: described oiliness raw material is one or both the mixture in Jojoba oil or isopropyl myristate.
7. Entermorpha crude polysaccharides protective skin cream according to claim 1, is characterized in that: described thickening agent is one or both mixture of high molecular cellulose or HANSHENGJIAO; Described solubilizing agent is quintessence oil emulsifying agent.
8. Entermorpha crude polysaccharides protective skin cream according to claim 1, is characterized in that: described freshener is Fructus Citri Limoniae quintessence oil Flos Rosae Rugosae quintessence oil, Garden lavender quintessence oil, Bergamot, rosemary ethereal oil, the mixture of one or more in Flos Pelargonii quintessence oil.
9. a preparation method for Entermorpha crude polysaccharides protective skin cream as claimed in claim 1, its step comprises:
(1), Entermorpha is immersed in to 12-24 hour in the ethanol that mass concentration is 90%-95%, filtration, oven dry; The Entermorpha of drying and distilled water are soaked to 2-4 hour according to the proportioning of 1g:25-45mL, smash rear 95 ℃ of heated at constant temperature 10-20 minute to pieces, cooling, cross leaching filtrate, after filtrate heating is concentrated, supernatant is got in centrifugalize; After ethanol precipitate with ethanol 24-28h, filter, taking precipitate lyophilizing, obtains Entermorpha crude polysaccharides;
(2), weigh good various raw materials according to formula;
(3), Entermorpha crude polysaccharides is added in deionized water constantly and stirs and make it swelling, add at glycerol post-heating to 70 ℃-90 ℃ of temperature and dissolve; Oiliness raw material is heating for dissolving under 70 ℃ of-90 ℃ of conditions, then both is mixed, and adds solubilizing agent, and emulsifying is stirred; Emulsion is cooled to 50-60 ℃, stirs after adding wetting agent, antioxidant, antiseptic, anti-allergic agent, nourishing additive agent; Emulsion is cooled to 40-45 ℃, adds tackifier, freshener to stir; The weight ratio of described Entermorpha crude polysaccharides and glycerol is 1-3:1.
10. the preparation method of Entermorpha crude polysaccharides protective skin cream according to claim 9, is characterized in that: in described step (1), Entermorpha is the one in bar Entermorpha, edge pipe Entermorpha or bent Entermorpha; In ethanol precipitate with ethanol, the mass concentration of alcoholic solution used is 75-80%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105919871A (en) * | 2016-06-01 | 2016-09-07 | 合肥丰瑞隆生物科技有限公司 | Armillaria mellea crude polysaccharide skincare product and preparation method thereof |
| CN109044942A (en) * | 2018-08-27 | 2018-12-21 | 项佳红 | A kind of multiple plant skin care item and preparation method thereof for moisturizing |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103044570A (en) * | 2013-01-25 | 2013-04-17 | 福建海兴保健食品有限公司 | Extracting process for efficiently extracting polysaccharide of enteromorpha |
| CN103396499A (en) * | 2013-08-14 | 2013-11-20 | 上海海洋大学 | Enteromorpha prolifera polysaccharide, and extraction method and application thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103044570A (en) * | 2013-01-25 | 2013-04-17 | 福建海兴保健食品有限公司 | Extracting process for efficiently extracting polysaccharide of enteromorpha |
| CN103396499A (en) * | 2013-08-14 | 2013-11-20 | 上海海洋大学 | Enteromorpha prolifera polysaccharide, and extraction method and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105919871A (en) * | 2016-06-01 | 2016-09-07 | 合肥丰瑞隆生物科技有限公司 | Armillaria mellea crude polysaccharide skincare product and preparation method thereof |
| CN109044942A (en) * | 2018-08-27 | 2018-12-21 | 项佳红 | A kind of multiple plant skin care item and preparation method thereof for moisturizing |
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