CN103798050A - Method for selecting Eucalyptus dunnii cold-resistant plants - Google Patents

Method for selecting Eucalyptus dunnii cold-resistant plants Download PDF

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CN103798050A
CN103798050A CN201410071459.0A CN201410071459A CN103798050A CN 103798050 A CN103798050 A CN 103798050A CN 201410071459 A CN201410071459 A CN 201410071459A CN 103798050 A CN103798050 A CN 103798050A
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dunnii
cold
seed
plant
low temperature
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CN103798050B (en
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罗先权
陈灵
饶红欣
彭静
彭信海
龚范武
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FOREST BOTANICAL GARDEN HUNAN PROV
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FOREST BOTANICAL GARDEN HUNAN PROV
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/22Improving land use; Improving water use or availability; Controlling erosion
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/40Afforestation or reforestation

Abstract

The invention belongs to the technical field of forestry, and discloses a method for selecting Eucalyptus dunnii cold-resistant plants. Eucalyptus dunnii seed seedlings are processed two hours at lower temperature in an artificial low-temperature climate room, then the seedlings are placed in the natural environment to be recovered and grow more than one month, plants without the freeze injury characteristic are selected for vegetative propagation, and after forestation contrast testing is carried out, the single plants with the good growth condition are selected, namely the cold-resistant plants are obtained. According to the method, the good cold-resistant plants can be selected only by two years, the selection time of the good Eucalyptus dunnii cold-resistant plants is shortened greatly, and selection is rapid. Meanwhile, the good Eucalyptus dunnii cold-resistant plants are younger in physical age, and cloning is facilitated. Meanwhile, the selection conditions can be manually controlled, influence of natural conditions is avoided, selection objects are tested under the same conditions, the comparability is high, the accuracy is good, and the cold-resistant plants can be selected. In addition, the selected good plants can at least resist low temperature below -7 DEG C, and are suitable for being popularized and planted in mid-subtropical areas.

Description

A kind of method of selecting E. dunnii hardy plant
Technical field
The invention belongs to forestry technology field, a kind of concrete method of selecting eucalyptus hardy plant, relates in particular to a kind of method of selecting E. dunnii hardy plant.
Background technology
Eucalyptus is the general designation that Myrtaceae (Myrtaceae) eucalyptus belongs to (Eucalyptus) plant, may originate from Cretaceous period end.Have more than 800 to plant, the original producton location overwhelming majority is in continent, Australia, and small part grows in contiguous New Guinea, Indonesia, and the Philippines.Eucalyptus majority is sub-hot plant, and happiness light, good wet, drought-enduring, heat resistanceheat resistant, can be grown in various soil, and most kinds can adapt to acid soil, also can adapt to alkaline soil, and optimum soil is fertile fluvisol.Eucalyptus tree performance grace, evergreen all the year round, growth failure is rapid, has rudiment upgrade and improve ability marshy, and tree crown is little, and evaporation of water is little, and water saving, should make Landscape Trees; And tree crown is little, light transmittance is high, is conducive to the growth of grass under grove, is also just conducive to the existence of insect and birds, is the good green tree species of convalescence area, residential quarter, hospital and public lawn.The eucalyptus florescence is long, and honey flow amount is large, is a kind of important nectariferous plant.Eucalyptus Wood character is varied, general tough and tensile durable, can supply the materials such as sleeper, ore pillar, bridge, building, pulp and wood-based plate; Leaf can be obtained through refining essential oil, produces eucalyptol, citral etc., has extensive use on spices, medical industry; The skin of many eucalyptus, material, leaf contain tannin, can be used for clean the boiler and set retarder.In addition eucalyptus or good fire wood.
Eucalyptus, from introducing a fine variety in 19th century to all over the world, has 96 countries to have cultivation at present.China introduces a fine variety existing more than 100 year history.In order to meet the demand of people to timber and wood fibre, eucalyptus has become the Major Tree Species Planted of south China cycle industrial material forest, and constantly moves northward to expand and plant.But because most of eucalyptus are not cold-resistant, limit moving northward of many fast-growing eucalyptus and planted.
E. dunnii (Eucalyptus dunnii Maiden) belongs to the eucalypt species that cold resistance is stronger, its material is good, rapidly, within 5 years, the raw diameter of a cross-section of a tree trunk 1.3 meters above the ground can reach 28cm in growth, and the height of tree reaches 17m, trunk is perfectly straight, flourishing, wooden even thickness, drought-resistant, disease resistance is strong, is applicable to very much the area plantation of China's middle subtropical zone.But because E. dunnii distributes limited in Australia, seed yield is few, and domestic E. dunnii is difficult to blossom and bear fruit, can not seeding, seed price costliness, the average price of seed that purity is higher is up to 70,000 yuan of kg-1, and E. dunnii seed genetic character is unstable, seed seedling cold resistance individual difference is remarkable, and forest form is irregular, has seriously restricted the development of these seeds.
The clones that adopt are carried out tree propagation more now.Vegetative propagation is the breeding that the nutrition organs such as the root that utilizes trees, stem, leaf, bud carry out.Vegetative kind is called clone.New the physical efficiency that vegetative propagation produces keeps the intrinsic characteristic of breeding, and offspring's proterties is consistent, and breeding time and growing way are more neat, young sprout size, hold tender property and color and luster more approaching, forest form is neat.E. dunnii also mainly adopts asexual process to carry out clone seedling afforestation at present.And the cold-resistant fine individual plant of How to choose E. dunnii is to carry out the key that clone is grown seedlings.
The system of selection in past is, seed seed is implanted in to planting site, under self-sow environment, through 3 to 5 years, experience the extreme low temperature time (as 2008 freezing) after select, Comparative Survey forest land, therefrom selects reasonable individuality, then this individual organ is carried out to clone by group training approach.With clone seedling afforestation, carry out final election, thereby determine the strong clone of cold resistance.Very long at select time according to this method, from being seeded into, the selection of cold-resistant fine individual plant often takes several years or the time of ten year, and the individual plant of now selecting is also because physiological age is large, is difficult to clone.On the other hand, forest land area is large, has microclimate, and the cold-resistant fine individual plant of selection has uncertainty.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of method of quick selection E. dunnii hardy plant.
For realizing object of the present invention, the present invention adopts following technical scheme:
A kind of method of selecting E. dunnii hardy plant, by E. dunnii seed seedling low temperature treatment 2 hours in artificial hypothermia's climatic chamber, then be placed under natural environment and recover growth more than 1 month, selection does not have the plant of freeze injury feature to carry out vegetative propagation, through Comparative Experiment on Afforestation, select the good individual plant of upgrowth situation to obtain cold-resistant plant.
Preferably, more than the height of seedling 10cm of described E. dunnii seed seedling.
Preferably, the breeding method of described E. dunnii seed seedling is for preparing container for plant growth, in-built Nutrition Soil, E. dunnii seed is mixed with fine earth and mix thoroughly on the rear Nutrition Soil evenly disseminating in container for plant growth, spray clear water drenched to Nutrition Soil, more than lasting tending management to the E. dunnii seed seedling height of seedling of clear water moisturizing afterwards reaches 10cm.
Preferably, described container for plant growth is the frame of plastic of the high 60cm × 40cm × 25cm of length and width, and pad is put nonwoven below, the high 10cm-15cm in four sides, Nutrition Soil thickness 12cm left and right.
Preferably, described E. dunnii seed is mixed with fine earth mix thoroughly on the rear Nutrition Soil evenly disseminating in container for plant growth be specially by the weight ratio of 1:50 and E. dunnii seed mixed with fine earth and mix thoroughly on the rear Nutrition Soil evenly disseminating in container for plant growth, below each container seed consumption 2g, then cover the loose ventilative thing that 1mm~3mm is thick.
Preferably, described artificial hypothermia's climatic chamber is that controlled minimum temperature can reach-10 ℃ or following phytotron.
Preferably, described low temperature treatment is to process under the low temperature of-7 ℃ to-10 ℃.
Preferably, described vegetative propagation is tissue cultivation or cuttage.
Preferably, after vegetative propagation, also comprise and will not have freeze injury feature E. dunnii seed seedling carry out the repetition low temperature treatment of at least 1 time and recover the step of growth.
Preferably, the temperature of described repetition low temperature treatment is lower than the temperature of a front low temperature treatment.
As shown from the above technical solution, the invention provides a kind of method of selecting E. dunnii hardy plant, by E. dunnii seed seedling low temperature treatment 2 hours in artificial hypothermia's climatic chamber, then be placed under natural environment and recover growth more than 1 month, selection does not have the plant of freeze injury feature to carry out vegetative propagation, through Comparative Experiment on Afforestation, select the good individual plant of upgrowth situation to obtain cold-resistant plant.The method of selection E. dunnii hardy plant of the present invention is placed E. dunnii seed seedling 2 hours under limiting temperature condition, thereby be then placed in recovery growth under natural environment and select cold-resistant excellent strain, as long as the time of 2 years just can be selected cold-resistant fine individual plant, greatly shorten the select time of the cold-resistant excellent strain of E. dunnii, selected fast.And the physiological age of the cold-resistant excellent strain of E. dunnii of the method for described selection E. dunnii hardy plant acquisition is little, is beneficial to clone.Simultaneously the method alternative condition of selection E. dunnii hardy plant of the present invention can manual control, is not subject to the impact of natural conditions, and participation in the election object is accepted the test under identical conditions, and comparativity is high, and accuracy is good, can choose cold-resistant excellent strain.In addition the excellent strain that the method for selection E. dunnii hardy plant of the present invention is selected is ability-7 ℃ following low temperature at least, is adapted at middle subtropical zone area popularizing planting.
Accompanying drawing explanation
Fig. 1 shows the schematic flow sheet of the method for selection E. dunnii hardy plant of the present invention.
Embodiment
The embodiment of the invention discloses a kind of method of selecting eucalyptus hardy plant.Those skilled in the art can use for reference content herein, suitably improve technological parameter and realize.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the artly, they are all deemed to be included in the present invention.Method of the present invention is described by preferred embodiment, and related personnel obviously can change method as herein described in content of the present invention, spirit and scope or suitably change and combination not departing from, and realizes and apply the technology of the present invention.
For realizing object of the present invention, the present invention adopts following technical scheme:
A kind of method of selecting E. dunnii hardy plant, by E. dunnii seed seedling low temperature treatment 2 hours in artificial hypothermia's climatic chamber, then be placed under natural environment and recover growth more than 1 month, selection does not have the plant of freeze injury feature to carry out vegetative propagation, through Comparative Experiment on Afforestation, select the good individual plant of upgrowth situation to obtain cold-resistant plant.
The method of selection E. dunnii hardy plant of the present invention is utilized the large characteristic of E. dunnii seed dissociation, the artificial cold climate condition of creating, E. dunnii seed seedling is processed 2 hours under limit cryogenic conditions, be then placed under natural environment and recover to grow, thereby select cold-resistant excellent strain.
Wherein, more than described E. dunnii seed seedling is preferably height of seedling 10cm.
E. dunnii seed seedling of the present invention adopts container nursery.
The breeding method of E. dunnii seed seedling is specially preparation container for plant growth in some embodiments, in-built Nutrition Soil, E. dunnii seed is mixed with fine earth and mix thoroughly on the rear Nutrition Soil evenly disseminating in container for plant growth, spray clear water drenched to Nutrition Soil, more than lasting tending management to the E. dunnii seed seedling height of seedling of clear water moisturizing afterwards reaches 10cm.
Wherein, described container for plant growth is preferably long × wide × high container for (30cm~50cm) × (50cm~70cm) × (20cm~40cm).Container can be plastic containers, shale container, can also be the container made from bamboo basket, peat, wood chip, brown paper, bark, potter's clay etc.And plastic containers can be flexible plastic, duroplasts and plastic film etc.
Further, described container for plant growth preferred container heelpiece has nonwoven or other analogs.
Further, in described container for plant growth, preferably pour into the Nutrition Soil that 30cm~15cm is thick, preferred, Nutrition Soil thickness 12cm.
Further, the Nutrition Soil in described container for plant growth is preferably loam or other and is beneficial to soil or the mixed-matrix of seed sprouting, as the homogeneous mixture of yellow soil and rice husk ash.
In some implementations, described container for plant growth is the frame of plastic of the high 60cm × 40cm × 25cm of length and width, and pad is put nonwoven below, the high 10cm-15cm in four sides, Nutrition Soil thickness 12cm left and right.Wherein, the high height that refers to frame of plastic four sides nonwoven in described four sides.Described Nutrition Soil is preferably yellow soil and rice husk ash homogeneous mixture, and rice husk ash consumption is 25v/v%~40v/v%.
Further, every square metre of E. dunnii seed consumption is 3g~4g.
In some implementations, described E. dunnii seed is mixed with fine earth mix thoroughly on the rear Nutrition Soil evenly disseminating in container for plant growth be specially by the weight ratio of 1:50 and E. dunnii seed mixed with fine earth and mix thoroughly on the rear Nutrition Soil evenly disseminating in container for plant growth, below each container seed consumption 2g, broadcast the loose ventilative thing that rear covering 2mm is thick.Wherein, described loose ventilative thing is preferably rice husk ash, peat soil; Cladding thickness is preferably 2mm.
Fine earth refers to that yellow soil grinds the soil that sieves or directly sieve.
The container nursery according to the method described above of E. dunnii seed, can germinate through 1 week left and right E. dunnii seed, more than after the tending management of 1 wheat harvesting period, E. dunnii seed seedling height of seedling can reach 10cm, and the selection of can resisting cold.
In some embodiments, the method of selection E. dunnii hardy plant of the present invention, E. dunnii select seeds is seeded in Nutrition Soil and grows seedlings under the natural environment of 17~27 ℃ of mean temperature of air, and excellent the selecting good strains in the field for seed of resisting cold under the natural environment below 15 ℃ at mean temperature of air after tending management selected (being that low temperature treatment and natural environment recover).As, it is example take Hunan, September-10 monthly mean temperature is 17~27 ℃ of seeding and seedling raisings in Nutrition Soil, can germinate through 1 week left and right E. dunnii seed, more than after the tending management of 1 wheat harvesting period, E. dunnii seed seedling height of seedling can reach 10cm, about November-December, 15 ℃ of following excellent selecting good strains in the field for seed of can resisting cold of mean temperature of air are selected, and E. dunnii seed seedling carries out can recovering by the natural environment below 15 ℃ at mean temperature of air after low temperature treatment in artificial hypothermia's climatic chamber.
In said vesse seedling raising process, to note water spray, shade and moisturizing, after E. dunnii seed seedling is unearthed, also need to carry out diseases prevention, mildew-resistant, insect protected management so that E. dunnii seed seedling Fast Growth.
The method of selection E. dunnii hardy plant of the present invention is manually created cold climate condition by artificial hypothermia's climatic chamber.The airtight xegregating unit of the factors such as phytotron refers to can Artificial Control illumination, temperature, humidity, air pressure and gas componant.Wherein, artificial hypothermia's climatic chamber of the present invention is preferably controlled minimum temperature and can reaches-10 ℃ or following phytotron.
The method of selection E. dunnii hardy plant of the present invention is by E. dunnii seed seedling low temperature treatment in artificial hypothermia's climatic chamber, and described low temperature treatment is preferably under the low temperature of-7 ℃ to-10 ℃ and processes, and can be-7 ℃ ,-8 ℃ ,-9 ℃ etc.
The method of selection E. dunnii hardy plant of the present invention is placed under natural environment and recovers to grow through low temperature treatment at E. dunnii seed seedling, within about 1 month, can see result through recover growth under natural environment, now most seedlings are freezed to death, and not dead is the cold-resistant excellent strain of primary election E. dunnii.
Recover to select do not have the plant of freeze injury feature to carry out vegetative propagation after growth.Described vegetative propagation is preferably tissue and cultivates or cuttage.
Further, in order to obtain more cold-resistant E. dunnii plant, the method for selection E. dunnii hardy plant of the present invention also comprises after vegetative propagation will not have freeze injury feature E. dunnii seed seedling to carry out the final election step of at least 1 time.
Further, described final election step specifically comprises low temperature treatment and recovers growth.
In some embodiments, E. dunnii seed seedling obtains the cold-resistant excellent strain of primary election E. dunnii through low temperature treatment with after recovering growth, after vegetative propagation, again in artificial hypothermia's climatic chamber, carry out low temperature treatment, under natural environment, recover afterwards growth, select not have the plant of freeze injury feature to be the cold-resistant excellent strain of E. dunnii of final election.
In other embodiments, the cold-resistant excellent strain of primary election E. dunnii after vegetative propagation through low temperature treatment with recover the cold-resistant excellent strain of E. dunnii that growth obtains final election, the cold-resistant excellent strain of E. dunnii of final election low temperature treatment and recover the cold-resistant excellent strain of E. dunnii that growth obtains secondary final election again after vegetative propagation.
Wherein, it is numerous that the excellent strain of E. dunnii hardy plant of just selecting is carried out needing further to expand after clone, transplants after taking root in the container for plant growth of Nutrition Soil is housed, and the moisturizing of shading is cultivated, and treats that the growth of clone seedling carries out final election above again up to 10cm.
More cold-resistant compared with the cold-resistant excellent strain of the E. dunnii of primary election for the E. dunnii hardy plant that final election is obtained, the temperature of the low temperature treatment in described final election step is lower than the temperature of the low temperature treatment of primary election.Preferred low 1 ℃~2 ℃.
Further, in the time of final election repeatedly, the temperature of preferably each low temperature treatment is lower than the temperature of a front low temperature treatment.At every turn can be low 0.5 ℃, low 1 ℃ or low 2 ℃ etc.
In some embodiments, the temperature of the cold-resistant excellent strain low temperature treatment of the method primary election E. dunnii of selection E. dunnii hardy plant of the present invention is-7 ℃, recovers to select not have the plant of freeze injury feature to be the cold-resistant excellent strain of E. dunnii of primary election after growth; The cold-resistant excellent strain of primary election E. dunnii is again carried out low temperature treatment at-8 ℃ after vegetative propagation, recovers afterwards growth under natural environment, selects not have the plant of freeze injury feature to be the cold-resistant excellent strain of E. dunnii of final election; The cold-resistant excellent strain of E. dunnii of final election is the cold-resistant excellent strain of E. dunnii in-9 ℃ of low temperature treatment and the final election of recovery growth acquisition secondary again after vegetative propagation.
In order further to understand the present invention, below in conjunction with embodiment, method provided by the invention is elaborated.
Embodiment 1: the method for selecting E. dunnii hardy plant
1, prepare artificial hypothermia's climatic chamber: set up controlled minimum temperature and can reach-10 ℃ or following artificial hypothermia's climatic chamber;
2, prepare container for plant growth: length × wide × height is the frame of plastic of 60cm × 40cm × 25cm, pad is put nonwoven below, the high 10cm-15cm of frame of plastic four sides nonwoven, Nutrition Soil thickness 10cm left and right, Nutrition Soil is yellow soil and rice husk ash homogeneous mixture, and rice husk ash consumption is 40v/v%;
3, container nursery: 17~27 ℃ of mean temperature of air, E. dunnii seed is evenly seeded in above-mentioned Nutrition Soil, and every square metre of 4g, broadcasts the thick rice husk ash of rear covering 2mm, water spray, the moisturizing of shading are carried out diseases prevention, mildew-resistant, insect protected and are managed so that its Fast Growth after sapling is unearthed;
4, low temperature treatment: move into artificial hypothermia's climatic chamber in the time that the growth of E. dunnii seed seedling is above up to 10cm, controlling temperature is below-7 ℃, low temperature treatment 2 hours in batches;
5, recover growth: the E. dunnii seed seedling after low temperature treatment is placed under natural conditions and is grown, can see result through about 1 month, now most seedlings are freezed to death, not dead is the cold-resistant excellent strain of primary election E. dunnii;
6, clone: cold-resistant primary election E. dunnii excellent strain is carried out to clone, further expand numerously, transplant in the plastics that Nutrition Soil is housed (or other materials) frame the moisturizing of shading after taking root;
7, final election: will grow up to clone seedling more than 10cm after 1 suboptimum strain clone, again move into artificial hypothermia's climatic chamber to December in batches, controlling temperature is below-9 ℃, the low temperature treatment selection of resisting cold for the second time for 2 hours, E. dunnii seed seedling after low temperature treatment is placed under natural conditions and is grown, can see result through about 1 month, now most seedlings are freezed to death, and not dead is the cold-resistant excellent strain of final election E. dunnii;
8, by cold-resistant final election E. dunnii excellent strain clone, through Comparative Experiment on Afforestation, select the good individual plant of upgrowth situation, organize training factorial seedling growth, be applied to production.
Embodiment 2: the method for selecting E. dunnii hardy plant
1, prepare artificial hypothermia's climatic chamber: set up controlled minimum temperature and can reach-10 ℃ or following artificial hypothermia's climatic chamber;
2, prepare container for plant growth: length × wide × height is the frame of plastic of 60cm × 40cm × 25cm, pad is put nonwoven below, the high 10cm-15cm of frame of plastic four sides nonwoven, Nutrition Soil thickness 12cm left and right, Nutrition Soil is yellow soil and rice husk ash homogeneous mixture, and rice husk ash consumption is 25v/v%;
3, container nursery: 17~27 ℃ of mean temperature of air, E. dunnii seed is evenly seeded in above-mentioned Nutrition Soil, and every square metre of 3g, broadcasts the thick peat soil of rear covering 2mm, water spray, the moisturizing of shading are carried out diseases prevention, mildew-resistant, insect protected and are managed so that its Fast Growth after sapling is unearthed;
4, low temperature treatment: move into artificial hypothermia's climatic chamber in the time that the growth of E. dunnii seed seedling is above up to 10cm, controlling temperature is below-7 ℃, low temperature treatment 2 hours in batches;
5, recover growth: the E. dunnii seed seedling after low temperature treatment is placed under natural conditions and is grown, can see result through about 1 month, now most seedlings are freezed to death, not dead is the cold-resistant excellent strain of primary election E. dunnii;
6, clone: cold-resistant primary election E. dunnii excellent strain is carried out to clone, further expand numerously, transplant in the plastics that Nutrition Soil is housed (or other materials) frame the moisturizing of shading after taking root;
7, final election: will grow up to clone seedling more than 10cm after 1 suboptimum strain clone, again move into artificial hypothermia's climatic chamber to December in batches, controlling temperature is below-8 ℃, the low temperature treatment selection of resisting cold for the second time for 2 hours, E. dunnii seed seedling after low temperature treatment is placed under natural conditions and is grown, can see result through about 1 month, now most seedlings are freezed to death, and not dead is the cold-resistant excellent strain of final election E. dunnii;
8, cold-resistant final election E. dunnii excellent strain is expanded numerous, repeating step 7, but temperature reduces by 1~2 ℃ while carrying out low temperature treatment, until leave 2~3 excellent strains;
9, by the excellent strain clone staying, through Comparative Experiment on Afforestation, select the good individual plant of upgrowth situation, organize training factorial seedling growth, be applied to production.
The explanation of above embodiment is just for helping to understand method of the present invention and core concept thereof.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of the claims in the present invention.

Claims (10)

1. select the method for E. dunnii hardy plant for one kind, it is characterized in that, by E. dunnii seed seedling low temperature treatment 2 hours in artificial hypothermia's climatic chamber, then be placed under natural environment and recover growth more than 1 month, selection does not have the plant of freeze injury feature to carry out vegetative propagation, through Comparative Experiment on Afforestation, select the good individual plant of upgrowth situation to obtain cold-resistant plant.
2. method according to claim 1, is characterized in that, more than the height of seedling 10cm of described E. dunnii seed seedling.
3. method according to claim 1, it is characterized in that, the breeding method of described E. dunnii seed seedling is for preparing container for plant growth, in-built Nutrition Soil, E. dunnii seed is mixed with fine earth and mix thoroughly on the rear Nutrition Soil evenly disseminating in container for plant growth, spray clear water drenched to Nutrition Soil, more than lasting tending management to the E. dunnii seed seedling height of seedling of clear water moisturizing afterwards reaches 10cm.
4. method according to claim 3, is characterized in that, described container for plant growth is the frame of plastic of the high 60cm × 40cm × 25cm of length and width, and pad is put nonwoven below, the high 10cm-15cm in four sides, Nutrition Soil thickness 12cm left and right.
5. method according to claim 3, it is characterized in that, described E. dunnii seed is mixed with fine earth mixes thoroughly on the rear Nutrition Soil evenly disseminating in container for plant growth, be specially by the weight ratio of 1:50 and E. dunnii seed mixed with fine earth and mix thoroughly on the rear Nutrition Soil evenly disseminating in container for plant growth, below each container seed consumption 2g, then cover the loose ventilative thing that 1mm~3mm is thick.
6. method according to claim 1, is characterized in that, described artificial hypothermia's climatic chamber is that controlled minimum temperature can reach-10 ℃ or following phytotron.
7. method according to claim 1, is characterized in that, described low temperature treatment is to process under the low temperature of-7 ℃ to-10 ℃.
8. method according to claim 1, is characterized in that, described vegetative propagation is tissue cultivation or cuttage.
9. method according to claim 1, is characterized in that, also comprises and will not have freeze injury feature E. dunnii seed seedling carry out the repetition low temperature treatment of at least 1 time and recover the step of growth after vegetative propagation.
10. method according to claim 9, is characterized in that, the temperature of described repetition low temperature treatment is lower than the temperature of a front low temperature treatment.
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Cited By (2)

* Cited by examiner, † Cited by third party
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CN104115677A (en) * 2014-07-16 2014-10-29 广西壮族自治区林业科学研究院 Method for selecting and establishing Eucalyptus dunnii Maiden grafting seed orchards
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