CN103749369B - Turbot and platichthys stellatus crossbreeding method - Google Patents
Turbot and platichthys stellatus crossbreeding method Download PDFInfo
- Publication number
- CN103749369B CN103749369B CN201410055279.3A CN201410055279A CN103749369B CN 103749369 B CN103749369 B CN 103749369B CN 201410055279 A CN201410055279 A CN 201410055279A CN 103749369 B CN103749369 B CN 103749369B
- Authority
- CN
- China
- Prior art keywords
- days
- parent
- turbot
- day
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a turbot and platichthys stellatus crossbreeding method which mainly includes the steps of (1) a turbot milter is selected as a male parent, a platichthys stellatus female fish is selected as a female parent, and the male parent and the female parent mature at the same time by the adoption of different conditions; (2) artificial insemination is carried out, wherein the insemination rate reaches 70%; (3) fertilized eggs deciduate and incubate at incubation water temperature of 8+/-0.5 DEG C in 80 hours, and the incubation rate is 82%; (4) larvae are cultured with intensified bait and then are cultured with compound feed gradually, and the survival rate of the larvae reaches 71%. According to the method, new varieties are provided for large-scale breeding of flatfish, and the method plays an important role in fish genetic breeding research.
Description
Technical field
The invention belongs to seawater fish seed breeding technical field, be specifically related to the cross breeding method of a kind of turbot and great flounder, be i.e. a kind of mating system possessing merit new varieties with turbot milter × great flounder raun artificial hybridization cultivation.
Background technology
Turbot (
scophthamus maximus) be the famous and precious tuna fisheries in Europe, it adapts to low temperature environment, growth is fast, strong stress resistance, fine and tender taste, colloid are abundant, mouthfeel is unique, dark liking by culturist and consumer.Introduce since China from 1992, develop rapidly northern China is coastal, become a pillar industry of northern China mariculture.Under propagating condition artificially, the turbot of domestication, by temperature control, control light, can realize annual breeding.Great flounder (
platichthyus stellatus) be cold warm nature fish, have wide temperature, wide salt, especially hypoxia-resistant capacity, low temperature ability and premunition are strong, and consumption rate is low, and cultivation survival rate is high, can the plurality of advantages such as overwintering.In addition its resistance to transport, resistance to freezing, after refrigeration, meat is substantially constant, is of high nutritive value, and mouthfeel is unique, as the primary raw material of senior raw fish, well received on market at home and abroad, has become emerging cultivation object.At present, have many reports about the hybridization of flounder flounder class, relevant turbot milter × there is not been reported for the hybridization of great flounder raun.
Summary of the invention
The object of this invention is to provide the cross breeding method of a kind of turbot and great flounder, namely control turbot milter by different temperature of cultivation, illumination and nutrition and great flounder raun sexual gland is synchronously grown; Use the method for artificial crossbreeding, make turbot milter and the hybridization of great flounder raun, cultivate new kind, thus make up the deficiencies in the prior art.
Cross breeding method of the present invention, includes following step:
1) parent population is selected
The male parent parent population selected is that form is normal, N/D, healthy 3 age turbot milter, selected by
Female parent be that form is normal, N/D, healthy 3 age great flounder raun;
2) parent fish rearing and genital regulating
Adopt different conditions to cultivate respectively the parent population of selection, regulate the synchronism of gonad development mainly through control temperature, illumination and nutrition.
3) artificial insemination and hatching
With conventional dry fertilization, after the 15min that has been fertilized, fertilized egg putting into is washed oopod, wash ovum with pure and fresh sterilization seawater, then ovum is filled it up with pure and fresh seawater and leave standstill, get floating ovum and hatch;
The incubation condition of fertilized egg: fertilized egg is at water temperature 8 ± 0.5 DEG C, salinity 28 ~ 30, pH7.8
~ 8.2, hatch under micro-venting condition;
4) Larva and fry and juvenile fish are cultivated
The cloth pond density 0.7 × 10 of the seedling of hatching
4tail/m
3, intensity of illumination 100 ~ 500lx; It is 8 DEG C that initial stage cultivates water temperature, and the 5th age in days water temperature raises 0.5 DEG C/2d to 16 DEG C gradually, remains unchanged afterwards, and water temperature diurnal variation is no more than 1 DEG C; Salinity is 28 ~ 32, pH value 8.0 ~ 8.2; Prelarva cloth pond depth of water 40cm, adds water to 90cm in 3rd ~ 7 days gradually, and within the 4th day, beginning equality of temperature every day changes water, and increases quantity of exchanged water gradually, and within the 10th day, rate of water exchange is 100% on the 30% ~ 40%, 20th day.Do not inhale the end before 7 days, inhale the end afterwards every day, remove dirt 1-2 time;
Chlorella is added in forward direction pond in prelarva cloth pond, keeps its density 2.0 × 10
5individual/milliliter; To throw something and feed during prelarva the 3rd day initial feeding wheel animalcule keep density 2 ~ 3/milliliter, about 12 days artemia naupliis of throwing something and feeding, within 12 ~ 15 days, keep artemia nauplii density 0.5 ~ 1/milliliter, after 15 days, increase to 1 ~ 2/milliliter gradually, stop wheel animalcule throwing something and feeding simultaneously;
Wheel animalcule, artemia nauplii to carry out the fortification of 16 hours with chlorella and the hardening agent that is rich in DHA, EPA and lecithin before throwing something and feeding.Within 18 days, induction prelarva ingests mixed bait.
The present invention, according to turbot milter and great flounder raun gonad development rule, adopts the method that fortification and environment conditioning combine, and induction parent population synchronism is ripe.The maternal great flounder of the present invention has the feature of wide temperature, wide salt, and adaptable with turbot filial generation, albefaction rate is low, and growth is fast, is applicable to promoting cultivation in a big way.The present invention is fertilized by dry method, and fertilization rate reached is to 70%, and incubation rate reaches 82%, survival rate 71%.
Embodiment
Below in conjunction with embodiment, method of the present invention is described in detail:
embodiment 1
1) determine parent: male parent selects turbot, female parent selects great flounder.
2) cultivation of male parent: select 3 age turbot parent fish 30 tail carry out parent population reinforced cultivating, culture density is 1 tail/m
2, raising water is allocated by seawater and well water and is formed, day quantity of exchanged water be 600%.Throw something and feed through the iced trash fish such as chilled sand lance, the orange red fish flesh of fish of sterilizing, throw something and feed day twice (7:00,16:00), according to the situation of ingesting, day feeding volume account for the 1-3.5% of its body weight, signal light control illumination, intensity of illumination 150-500lx, light application time is by 10L/14D to 8L/16D.Water temperature is adjusted to 12 DEG C gradually by 8 DEG C, first 30 days of reinforced cultivating, the sexual gland getting 1 tail fish for every 10 days carries out range estimation and sexual gland developmental state is determined in histologic section analysis, compare with the gonad development situation of female parent, each intensification 0.5 DEG C, along with expanding of belly, observe belly developmental state and heat up once for every 6 days, each intensification 0.5 DEG C to 12 DEG C.
Maternal cultivation: select 3 age great flounder parent population 20 tail carry out parent population reinforced cultivating, culture density is 1 tail/m
2, raising water is allocated by seawater and well water and is formed, day quantity of exchanged water be 600%.Throw something and feed through chilled yellow croaker, sardine, the oyster meat etc. of sterilizing, 0.5-2 ‰ vitamin C is added in per kilogram feed, 0.3-0.4 ‰ vitamin E, the B B-complex of 0.8 ‰, the DHA of 0.6%, the lecithin of 1.2%, the zinc sulphate of 0.2 ‰, day to throw something and feed twice (7:00,16:00), day feeding volume account for the 1-3% of its body weight.With the illumination of signal light control parent fish pond, intensity of illumination 150-360lx, light application time is by 10L/14D to 16L/8D.Water temperature is adjusted to 8 DEG C gradually by 6 DEG C, first 30 days of reinforced cultivating, and the sexual gland getting 1 tail fish for every 10 days carries out range estimation and sexual gland developmental state is determined in histologic section analysis, heats up 0.5 DEG C at every turn.Observe gonad development situation, gonad development to water temperature during the fourth phase rise to 8 DEG C constant.
3) artificial hybridization fertilization
By parent population wet towel parcel, with absorbent cotton by gonopore wiped clean, both hands in parent population belly both sides simultaneously evenly firmly, extrude belly slowly from rear and front, namely have seminal fluid or ovum to flow out.Seminal fluid is held in the dry beaker of sterilization stand-by.2000 ml beakers through sterilization are used to access ovum.
Artificial dry method insemination: seminal fluid instillation is filled in the beaker of ovum, the seawater limit that limit slowly adds pure and fresh sterilization is stirred, whipping process approximately continues 5-6 minute, leave standstill after 15 minutes, that fertilized egg is put into 80 mesh sieve thin,tough silk washes oopod, wash ovum 3 times with pure and fresh sterilization seawater (8 ± 0.5) DEG C, then ovum is poured into 2000 milliliters of graduated cylinders and fill it up with pure and fresh seawater standing 30 minutes.
Incubating oosperm: get floating ovum and be placed in the hatching of 1 × 0.5m net cage, hatch under water temperature 8 ± 0.5 DEG C, salinity 28 ~ 30, pH7.8 ~ 8.2, micro-venting condition, rear demoulding hatching in 80 hours, incubation rate is 82%.
4) Larva and fry and juvenile fish are cultivated
Water for larval nursing is sand filtration seawater and well water.Newly hatched larvae hydrostatic, micro-inflation are cultivated, cloth pond density 0.7 × 10
4tail/m
3, along with growth reduces density gradually.Intensity of illumination 100 ~ 500lx.It is 8 DEG C that initial stage cultivates water temperature, and the 5th age in days water temperature raises 0.5 DEG C/2d to 16 DEG C gradually, remains unchanged afterwards, and water temperature diurnal variation is no more than 1 DEG C.Salinity is 28 ~ 32, pH value 8.0 ~ 8.2.Prelarva cloth pond depth of water 40cm, adds water to 90cm in 3rd ~ 7 days gradually, and within the 4th day, beginning equality of temperature every day changes water, and increases quantity of exchanged water gradually, the 10th day rate of water exchange 30% ~ 40%, and rate of water exchange raises gradually, is 200% to 20 days rate of water exchange.Do not inhale the end before 7 days, inhale the end afterwards every day, remove dirt 1-2 time.
Feed: chlorella is added in forward direction pond in prelarva cloth pond, keeps its density 2.0 × 10
5individual/milliliter.Prelarva the 3rd day initial feeding, the wheel animalcule keep density 2 ~ 3/milliliter of now throwing something and feeding.About 12 days artemia naupliis of throwing something and feeding, keep artemia nauplii density 0.5 ~ 1/milliliter, increase to 1 ~ 2/milliliter gradually after 15 days for 12 ~ 15 days, stop wheel animalcule throwing something and feeding simultaneously.Wheel animalcule, artemia nauplii need the fortification carrying out 16 hours with chlorella and the hardening agent that is rich in DHA, EPA and lecithin before throwing something and feeding.Feed material is increased gradually according to the situation of ingesting.
Adopt this breeding method male and female parent population substantially can reach synchronous maturation, and the ovum quality of raun is higher, natural rate of interest is 83%, fertilization rate is 70%, and incubation rate reaches more than 82%, and seedling percent reaches 71%, growth is very fast, abnormal rate is 3.5%, and albefaction rate is few, cultivates high-quality seedling 800,000 tail that 60 days obtain the long 4.5cm of average body.
Claims (2)
1. a cross breeding method for turbot and great flounder, described cross breeding method is hybridized as parent with turbot milter and great flounder raun; It is characterized in that, include following step:
(1) parent population is selected:
The male parent parent population selected is that form is normal, N/D, healthy 3 age turbot milter, selected female parent is that form is normal, N/D, healthy 3 age great flounder raun;
(2) parent fish rearing and genital regulating:
The parent population of selection is cultivated, regulates the synchronism of gonad development;
(3) artificial insemination and hatching:
With conventional dry fertilization, after the 15min that has been fertilized, fertilized egg putting into is washed oopod, wash ovum with pure and fresh sterilization seawater, then ovum is filled it up with pure and fresh seawater and leave standstill, get floating ovum and hatch; The incubation condition of described fertilized egg is as follows: fertilized egg is hatched under water temperature 8 ± 0.5 DEG C, salinity 28 ~ 30, pH7.8 ~ 8.2, micro-venting condition;
(4) Larva and fry and juvenile fish incubation step:
The cloth pond density of hatching seedling is 0.7 × 10
4tail/m
3, intensity of illumination 100 ~ 500lx; Initial stage cultivates water temperature 8 DEG C, and the 5th age in days water temperature raises 0.5 DEG C/2d to 16 DEG C gradually, remains unchanged afterwards, and water temperature diurnal variation is no more than 1 DEG C; Salinity is 28 ~ 32, pH value 8.0 ~ 8.2; Prelarva cloth pond depth of water 40cm, adds water to 90cm in 3rd ~ 7 days gradually, and within the 4th day, beginning equality of temperature every day changes water, and increases quantity of exchanged water gradually, and within the 10th day, rate of water exchange is 100% on the 30% ~ 40%, 20th day; Do not inhale the end before 7 days, inhale the end afterwards every day, remove dirt 1-2 time;
Chlorella is added in forward direction pond in prelarva cloth pond, keeps its density 2.0 × 10
5individual/milliliter; To throw something and feed during prelarva the 3rd day initial feeding wheel animalcule keep density 2 ~ 3/milliliter, about 12 days artemia naupliis of throwing something and feeding, within 12 ~ 15 days, keep artemia nauplii density 0.5 ~ 1/milliliter, after 15 days, increase to 1 ~ 2/milliliter gradually, stop wheel animalcule throwing something and feeding simultaneously.
2. the cross breeding method as described in claim 1, is characterized in that, described wheel animalcule, artemia nauplii to carry out the fortification of 16 hours with chlorella and the hardening agent that is rich in DHA, EPA and lecithin before throwing something and feeding; Within 18 days, start to induce prelarva to ingest mixed bait.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410055279.3A CN103749369B (en) | 2014-02-19 | 2014-02-19 | Turbot and platichthys stellatus crossbreeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410055279.3A CN103749369B (en) | 2014-02-19 | 2014-02-19 | Turbot and platichthys stellatus crossbreeding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103749369A CN103749369A (en) | 2014-04-30 |
| CN103749369B true CN103749369B (en) | 2015-07-01 |
Family
ID=50516828
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410055279.3A Expired - Fee Related CN103749369B (en) | 2014-02-19 | 2014-02-19 | Turbot and platichthys stellatus crossbreeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103749369B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105639210B (en) * | 2016-03-30 | 2018-07-06 | 全国水产技术推广总站 | A kind of feed and its application process for cultivating ecology mark type lefteye flounder |
| CN108541633B (en) * | 2018-06-29 | 2020-10-30 | 中国科学院海洋研究所 | Light environment regulation and control method for improving hatching rate of fertilized eggs of turbot and growth of larval and juvenile fishes |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1539270A (en) * | 2003-10-29 | 2004-10-27 | 中国海洋大学 | Method for cross breeding between bothid and plaice |
| CN100355337C (en) * | 2005-04-08 | 2007-12-19 | 中国科学院海洋研究所 | Artificial hybridization and breeding method for male Paralichthys dentatus and female summer flounder |
| CN101695286A (en) * | 2009-10-13 | 2010-04-21 | 中国水产科学研究院黄海水产研究所 | Juvenile turbot culturing and feeding method |
| CN101766141A (en) * | 2010-02-05 | 2010-07-07 | 北京市水产科学研究所 | Healthy culture method for paralichthys lethostigma |
-
2014
- 2014-02-19 CN CN201410055279.3A patent/CN103749369B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN103749369A (en) | 2014-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101919359B (en) | Method for comprehensively regulating and controlling gonad mature ovulation of cheilinus undulates | |
| CN103081840B (en) | Limanda aspera parent fish rearing and hatching method | |
| CN106172117B (en) | Little yellow croaker raun and Larimichthys crocea milter cross breeding method | |
| CN104823883A (en) | Macrobranchium rosenbergii larvae breeding method | |
| CN103404462B (en) | Method for cultivating fries of coilia ectenes in soil pond | |
| CN103621442B (en) | The indoor full artificial breeding method of a kind of jellyfish | |
| CN103918615B (en) | A kind of method of the red clam worm of indoor circulating water three-dimensional culture | |
| CN105494193A (en) | Seedling culture method of Sanguinolaria diphos | |
| CN104604759A (en) | Grass carp culturing method | |
| CN103004656A (en) | Intensified artificial breeding method for Australian crayfishes | |
| CN105360026A (en) | Method for freshwater shrimp cultivation | |
| CN104304103B (en) | A kind of doing sth. in advance breeds, cultivates big specification lip * offspring breeding method | |
| CN104542407A (en) | Two-stage cultivation method for loach offspring seeds | |
| CN101703011B (en) | Method for cultivating synechogobius hast fry | |
| CN102144596B (en) | Method for hybridizing female verasper variegatus and male verasper moseri for breeding and fry cultivating | |
| CN104719196A (en) | Breeding method of zebra fish | |
| CN103262819B (en) | Crossbreeding and seedling method of odontobutis potamophila and odontobutis yaluensis | |
| CN101444192B (en) | Sebastiscus marmoratus industrialized fry breeding method | |
| CN100355337C (en) | Artificial hybridization and breeding method for male Paralichthys dentatus and female summer flounder | |
| CN113557998B (en) | Ecological breeding method for high-quality shrimps | |
| CN104285855A (en) | Large-scale xenocypris davidi bleeker artificial reproduction method | |
| CN103749369B (en) | Turbot and platichthys stellatus crossbreeding method | |
| CN101401555B (en) | Industrialized fingerling cultivation method for late cod croaker shaped spotted maigre and special clean fish hardening agent thereof | |
| CN102792910B (en) | Cultivating method of oncorhynchus mykiss walbaum gynoecial hybrid offspring seed | |
| CN106035176A (en) | Cultivation technique of freshwater shrimps |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150701 Termination date: 20160219 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |