CN103733875B - Cordyceps militaris industrial production method and technique - Google Patents
Cordyceps militaris industrial production method and technique Download PDFInfo
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Abstract
The invention provides a kind of Cordyceps militaris industrial production method and technique, comprise the following steps: introduce and separate mother's kind；Go out grass test;Female kind expanding propagation；Make original seed；Make liquid cultivation seed, configure culture medium simultaneously；Sterilizing also cools down；Inoculation；Production management: send out bacterium；See that light is cultivated；Ventilate；Miscellaneous bacteria, pest control；Physical control；Chemical prevention；Gather；Manage after adopting；Packaging, transport；Storage；Having yield high, the benefits such as simple to operate, efficiency is high, it is simple to large-scale production, the Cordyceps militaris of output is economical and practical, and production cost is low, be prone to storage and transport.
Description
Technical field
The present invention relates to health care of food product processing technique field, particularly to a kind of Cordyceps militaris industrial production method and work
Skill.
Background technology
Cordyceps militaris (scarlet caterpillar fungus) is the abbreviation of north Cordyceps, is also Cordyceps militaris (L.) Link. or Cordyceps militaris (L.) Link.,
Popular name Herba Cynomorii, is worm, the medicinal fungi of bacterium combination, is the Stroma of Cordyceps militaris (L.) Link., i.e. sporophore, by the most organized mycelium group
Become.Cordyceps militaris (L.) Link. belongs to Eumycota, Ascomycetes, Hypocreales, Clavicipitaceae, Cordyceps, belongs to modern Valuable Herbal Medicine,
Primary growth is at the northern area of China.Cordyceps militaris does not contain only rich in protein and aminoacid, and containing more than 30 kind of people
Trace element needed for body, is first-class excellent tonic product.
Cordyceps militaris (L.) Link. contain rich in protein, 18 kinds of aminoacid, 17 kinds of trace element, 12 kinds of vitamin: A, B1,
B2, B6, B12, C, D, E equal size is higher than mushrooms, single through Hong Kong, Korea S, Japan, Canada, the U.S. and more than ten, domestic state
Research and the detection of the countries in the world such as position prove, Cordyceps militaris is the Ideal Substitute of tradition Cordyceps, and cordycepin is to people's
Nose, pharyngeal cancer (KD) cell have the strongest inhibitory action.Using cordycepin mostly to be adjuvant therapy of malignant tumor clinically, symptom obtains
Improve more than 91.7%;It is mainly used in rhinocarcinoma, pharyngeal cancer, pulmonary carcinoma, leukemia, the brain cancer, intestinal cancer, gastric cancer and other are pernicious swollen
The patient of tumor.
Along with Cordyceps militaris is the most medicinal and being found of edibility, its demand is more and more higher, but due to
Cordyceps requires extremely harsh for working condition, thus the most all cannot large-scale production, at controlled environment bar
Under part, utilize complete set and complex art to realize the large-scale production of annual, seldom have technology to reach.
Summary of the invention
Cannot satisfy social needs for prior art, lack effective large-scale production Cordyceps militaris technology above-mentioned lack
Falling into and problem, the purpose of the embodiment of the present invention is to provide a kind of Cordyceps militaris industrial production method and technique, at controlled environment
Under the conditions of, utilize complete set and complex art to realize the large-scale production of annual.
In order to achieve the above object, the following technical scheme of embodiment of the present invention offer:
A kind of Cordyceps militaris industrial production method and technique, it is characterised in that comprise the following steps:
Q1: introduce and separate mother and plant: by the mycelium pure culture that obtain through various method selection-breeding, there is fecundity and
Secondary culture is picked as female kind, utilizes the method for sterile working to plant from the micropopulation mixed independent by required mother
Separate；
Q2: go out grass test: prove that strain can produce the qualified son of economical character under normal conditions through experiment in cultivation real
Body;
Q3: female kind expanding propagation: aseptically, cuts into 6mm～8mm fritter by the female slant culture of planting separated, turns
Receive on PDA slant medium, at 18 DEG C～22 DEG C, carry out light culture, preserve in 4 DEG C of environment after mycelia covers with test tube；
Q4: make original seed；
Q5: make liquid cultivation seed, configure culture medium simultaneously；
Q6: sterilizing also cools down: after autoclave temperature reaches 121 DEG C～126 DEG C, keeps 1h, then by normal-pressure sterilization temperature
Degree reaches 100 DEG C～105 DEG C, keeps being not less than 8h, when in autoclave, culture medium temperature takes the dish out of the pot when being down to 40 DEG C～50 DEG C, moves into
In transfer room, it is cooled to less than 25 DEG C and inoculates；
Q7: inoculation: first by inoculating tool alcohol burner flame calcination sterilizing, then liquid production kind is covered cooling
In solid medium, every bottle about contains 35g solid medium, can inoculate 5ml liquid cultivation seed；
Q8: production management: the production cycle is about 45 days；
Q81: send out bacterium: postvaccinal cultivation, based in culture bottle, is taken advantage of with pallet to be positioned over and sent out bacterium room, can 2～5 stackings put,
Temperature controls between 18 DEG C～22 DEG C, and relative air humidity controls between 60%～70%, dark culturing, ventilation,
Keep air fresh；
Q82: see that light is cultivated: see light by added for the culture bottle covering with mycelia under the conditions of light culture is Artificial Control Indoor Temperature, wet
Degree and intensity of illumination, temperature is maintained at 18 DEG C-22 DEG C, and humidity is maintained at 60-75%；
Q83: ventilate: keep room air fresh, ventilation of regularly windowing every day；
Q84: miscellaneous bacteria, pest control:
Q841: physical control: regulate cultivation indoor temperature and humidity in production process, it is to avoid overdrying or humidity, strengthens
Ventilation；
Vent installs gauze and the outer bactericidal unit of purple lamp, and preventing and treating demodicid mite class, insects and miscellaneous bacteria enter；
Remove immediately and autoclaving after finding polluter in workshop, prevent and treat secondary pollution；
Ultraviolet light is used to carry out disinfection in whole culturing room under conditions of without culture；
Q842: chemical prevention: use under conditions of without culture efficiently, low toxicity chemical agent carries out thorough disinfection；
Long more than the 5cm of Q9: gather: sporophore, top forms Sporangium and gathers during without spores flying；Son is pinned real with hands
Body base portion is adopted down, and vessel is advisable with shallow bid, in order to avoid extruding；Manage after adopting: the culture bottle after gathering empties, scrubs sterilizing, can
Recycling；
Q10: pack, transport: avoid Exposure to Sunlight, drench with rain, prevent from falling hit, weight, forbid with poisonous, be harmful to, have abnormal flavour article
Mixed fortune；
Q11: storage: the Cordyceps militaris after gathering can 50 DEG C of drying in drying baker, water content is less than in the case of 10% 10 DEG C
～can preserve 12 months at 25 DEG C；Storage house should meet rainproof, protection against rodents, insect protected, ventilate, be dried, clean, shady and cool, without sunlight
The requirement of direct projection, forbid with poisonous, be harmful to, be corrosive, have abnormal flavour, the article that easily pollute are mixed puts.
Preferred as technique scheme, the female prescription separated of planting in described Q1 step should meet:
Preferred as technique scheme, the female prescription separated of planting in described Q1 step should meet:
| Project | Female kind Microbiological requirements |
| Hypha form | Uniformly, thread |
| Miscellaneous bacteria | Nothing |
Preferred as technique scheme, making the prescription that mother plants and should meet in described Q4 step:
Preferred as technique scheme, the organoleptic requirements of the liquid cultivation seed in described Q5 step should meet:
Preferred as technique scheme, being respectively as follows: of the configuration culture medium in described Q5 step
Mother plants and Medium for original variety is: Rhizoma Solani tuber osi, glucose, agar culture medium, specifically comprises: Rhizoma Solani tuber osi, Fructus Vitis viniferae
Sugar, potassium dihydrogen phosphate, magnesium sulfate, vitamin, agar powder, water；
Liquid cultivation seed culture medium prescription is: Rhizoma Solani tuber osi, glucose culture solution, specifically comprises: Rhizoma Solani tuber osi, glucose, phosphorus
Acid dihydride potassium, magnesium sulfate, vitamin, water；
Culture medium for producing: Semen Tritici aestivi, liquid cultivation seed PDA culture fluid, pH value 6.0～7.0, animal proteinum is Pupa bombycis
Or egg.
Preferred as technique scheme, female plants and Medium for original variety specifically comprises: Rhizoma Solani tuber osi 200g, glucose
20g, potassium dihydrogen phosphate 7.0g, magnesium sulfate 3.5g, vitamin B10.1g, agar powder 20g, water 1000mL, pH value is 6.0～7.0；
Liquid cultivation seed culture medium prescription, particularly as follows: Rhizoma Solani tuber osi 200g, glucose 20g, potassium dihydrogen phosphate 7.0g, magnesium sulfate 3.5g, is tieed up
Raw element B10.1g, water 1000mL, pH value is 6.0～7.0；The standard culture based formulas of each culture bottle for producing is concrete
For: Semen Tritici aestivi 35g, liquid cultivation seed PDA culture fluid 50mL, pH6.0～7.0；Animal proteinum 20g.
Preferred as technique scheme, Cordyceps militaris industrial production method and technique, it produces equipment and produces ring
Border is:
Production equipment is: balance, high-pressure sterilizing pot, superclean bench or inoculating hood, calorstat, microscope and alcohol burner；
Factory Building sets Feedstock treating room, spice room, subpackage room, sterile chamber, cooling chamber, connects bacterium room, bacterium room, culturing room, storeroom, packaging
Room;
Factory Building internal medium is divided into bacteria district and aseptic area, and aseptic area arranges uviol lamp, and for every day, timing carries out physics
Sterilization；Workshop is outside uses the temperature control facilities such as warming facility, internal employing air-conditioning, it is ensured that indoor temperature controls to 18
DEG C～22 DEG C between；
Culturing rack is provided with is satisfied with the light source cultivating sporophore, and intensity of illumination controls between 500LUX～1000LUX；
There is special vent in every culture room, filtration wind apparatus, indoor CO need to be installed at vent2Concentration is not higher than 1000PPM.
The Cordyceps militaris industrial production method of present invention offer and technique, have yield high, and simple to operate, efficiency is high good
Place, it is simple to large-scale production, the Cordyceps militaris of output is economical and practical, and production cost is low, be prone to storage and transport.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
In having technology to describe, the required accompanying drawing used is briefly described, it should be apparent that, the accompanying drawing in describing below is only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, also may be used
To obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is Cordyceps militaris industrial production method and the process step schematic diagram of technique of the embodiment of the present invention.
Detailed description of the invention
Below in conjunction with the accompanying drawing of the present invention, technical scheme is clearly and completely described, it is clear that institute
The embodiment described is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention,
The every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, broadly falls into this
The scope of bright protection.
Term and definition
Female plant (stock culture): the mycelium pure culture with fecundity that obtains through various method selection-breeding and
Its secondary culture;Also referred to as one-level kind, test tube kind.
Original seed (mother spawn): planted the mycelium pure culture of transplanting, amplification culture by mother, also referred to as two grades
Kind.
Liquid cultivation seed (liquid spawn): by the transplanting of solid original seed, the pure mycelia liquid culture of amplification culture
Thing, culture vessel is vial or fermentation tank, is served only for production and cannot be used for strain expanding propagation.
Miscellaneous bacteria (weed mould): food (medicine) causes the microorganism of pollution with bacterium in cultivating.
Strain separating (culture isolation): utilize the method for sterile working by required edible fungi from mixing
Micropopulation in be separately separated out.
Strain purifies the method that (purification culture) obtains pure culture.
Rejuvenation of spawn (rejuvenation) stock breeding prevents and treats the technical measures of spawn degeneration.
Sporophore (fruit body): produce sporogenic fungal tissue organ.
Embodiment 1
Fig. 1 is Cordyceps militaris industrial production method and the process step schematic diagram of technique of the embodiment of the present invention, and step is such as
Shown in lower:
1. introduce and separate mother's kind
1.1 introduce: introduce variety certification committee national or above the provincial level by the kind assert.
1.2 separate: female prescription separated of planting should meet the regulation of Tables 1 and 2.
Table 1
Table 2
| Project | Female kind Microbiological requirements |
| Hypha form | Uniformly, thread |
| Miscellaneous bacteria | Nothing |
2. go out grass test
Introduce and have the unit of strain production qualification and enterprise to be supplied female kind or separation mother's kind, need to be proved it through experiment in cultivation
Go out grass and the qualified rear of economical character can be used for breeding.
3. female kind expanding propagation
Under aseptic condition, the female slant culture of planting separated is cut into 6mm～8mm fritter, is transferred to the training of PDA inclined-plane
Support on base (see appendix A .1), light culture at 18 DEG C～22 DEG C, 4 DEG C of preservations after mycelia covers with test tube.
4. make original seed
4.1 containers size: the regulation of 4.7.1.2 in NY/T528 should be met.
4.2 prescriptions: prescription should meet the regulation of table 3.
Table 3
5. make liquid cultivation seed
5.1 containers: using the high-temperature resistant container that specification is consistent, container is without breakage.
5.2 prescriptions: prescription should meet the regulation of table 4.
Table 4
| Project | The organoleptic requirements of liquid cultivation seed |
| Mycelia | Mycelia is cotton-shaped or coccoid, and uniformity is faint yellow |
| Impurity | Nothing |
| Culture fluid | Bacterium solution clear, fungus ball is uniformly dispersed, without floating, without precipitation in bacterium solution |
| Abnormal smells from the patient | There are the exclusive bacterium fragrance of Cordyceps militaris, free from extraneous odour |
| Microscopy | Hyphal diameter and branch uniformity, without other fungus and antibacterial |
6. preparation culture medium
Being respectively as follows: of 6.1 configuration culture medium
Mother plants and Medium for original variety is: Rhizoma Solani tuber osi, glucose, agar culture medium, specifically comprises: Rhizoma Solani tuber osi 200g,
Glucose 20g, potassium dihydrogen phosphate 7.0g, magnesium sulfate 3.5g, vitamin B1 0.1g, agar powder 20g, water 1000mL, pH value is
6.0～7.0；
Liquid cultivation seed culture medium prescription is: Rhizoma Solani tuber osi, glucose culture solution, specifically comprises: Rhizoma Solani tuber osi 200g, glucose
20g, potassium dihydrogen phosphate 7.0g, magnesium sulfate 3.5g, vitaminB10 .1g, water 1000mL, pH value is 6.0～7.0；
Culture medium for producing: Semen Tritici aestivi, liquid cultivation seed PDA culture fluid, pH value 6.0～7.0, animal proteinum is Pupa bombycis
Or egg；For the standard culture based formulas of each culture bottle that produces particularly as follows: Semen Tritici aestivi 35g, liquid cultivation seed PDA culture fluid
50mL, pH6.0～7.0；Animal proteinum 20g.
7. sterilizing: after autoclave temperature reaches 121 DEG C～126 DEG C, keeps 1h.Normal-pressure sterilization temperature reach 100 DEG C～
105 DEG C, it is not less than 8h.
8. cooling: when in autoclave, culture medium temperature takes the dish out of the pot when being down to 40 DEG C～50 DEG C, moves in transfer room, is cooled to 25
Inoculate below DEG C.
9. inoculation: first by inoculating tool alcohol burner flame calcination sterilizing, then liquid production kind is covered cooling
In solid medium, every bottle of 35g solid medium, 5ml liquid cultivation seed can be inoculated.
10. production management
10.1 bacterium: postvaccinal cultivation, based in culture bottle, is taken advantage of with pallet to be positioned over and sent out bacterium room, can 2～5 stackings put,
Temperature controls between 18 DEG C～22 DEG C, and relative air humidity controls between 60%～70%, dark culturing, ventilation,
Keep air fresh.
10.2 see that light is cultivated: see light by added for the culture bottle covering with mycelia under the conditions of light culture, Artificial Control Indoor Temperature, wet
Degree and intensity of illumination, temperature is maintained at 18 DEG C-22 DEG C, and humidity is maintained at 60-75%.
10.3 ventilate: keep room air fresh, ventilation of regularly windowing every day.
10.4 miscellaneous bacterias, pest control
10.4.1 physical control:
Production process regulates cultivation indoor temperature and humidity, it is to avoid overdrying or humidity, strengthen ventilation；
Vent installs gauze and the outer bactericidal unit of purple lamp, and preventing and treating demodicid mite class, insects and miscellaneous bacteria enter；
Remove immediately and autoclaving after finding polluter in workshop, prevent and treat secondary pollution；
Ultraviolet light is used to carry out disinfection in whole culturing room under conditions of without culture.
10.4.2 chemical prevention: use under conditions of without culture efficiently, low toxicity chemical agent carries out thorough disinfection.
11. gather
11.1 standards: long more than the 5cm of sporophore, top forms Sporangium and gathers during without spores flying
11.2 methods: pinning sporophore base portion with hands and adopt down, vessel is advisable with shallow bid, in order to avoid extruding.
11.3 adopt after manage: the culture bottle after gathering empties, scrubs sterilizing, repeatable utilization.
12. packagings: product external packaging should meet the regulation of GB/T6543.
13. transports: avoid during transport Exposure to Sunlight, drench with rain, prevent from falling hit, weight, forbid with poisonous, be harmful to, have abnormal flavour article
Mixed fortune.
14. storages
14.1 dry: the Cordyceps militaris after gathering can 50 DEG C of drying in drying baker, water content is less than in the case of 10% 10 DEG C
～can preserve 12 months at 25 DEG C.
14.2 storage houses: storage house should meet rainproof, protection against rodents, insect protected, ventilate, be dried, clean, shady and cool, without sunlight
The requirement of direct projection, forbid with poisonous, be harmful to, be corrosive, have abnormal flavour, the article that easily pollute are mixed puts.
The above, the only detailed description of the invention of the present invention, but protection scope of the present invention is not limited thereto, and any
Those familiar with the art, in the technical scope that the invention discloses, can readily occur in change or replace, should contain
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should described be as the criterion with scope of the claims.
Claims (8)
1. a Cordyceps militaris industrial production method, it is characterised in that comprise the following steps:
Q1: introduce and separate mother's kind: by the mycelium pure culture that obtain through various method selection-breeding, there is fecundity and subculture thereof
Culture is picked as female kind, utilizes the method for sterile working required female kind to be separately separated from the micropopulation mixed
Out；
Q2: go out grass test: prove that strain is under normal conditions to produce the qualified sporophore of economical character through experiment in cultivation；
Q3: female kind expanding propagation: aseptically, cuts into 6mm～8mm fritter by the female slant culture of planting separated, is transferred to
On PDA slant medium, at 18 DEG C～22 DEG C, carry out light culture, preserve in 4 DEG C of environment after mycelia covers with test tube；
Q4: make original seed；
Q5: make liquid cultivation seed, configure culture medium simultaneously；
Q6: sterilizing also cools down: after autoclave temperature reaches 121 DEG C～126 DEG C, keeps 1h, then normal-pressure sterilization temperature is reached
To 100 DEG C～105 DEG C, keep being not less than 8h, when in autoclave, culture medium temperature takes the dish out of the pot when being down to 40 DEG C～50 DEG C, move into inoculation
Indoor, are cooled to less than 25 DEG C and inoculate；
Q7: inoculation: first by inoculating tool alcohol burner flame calcination sterilizing, then liquid cultivation seed is covered the solid of cooling
In culture medium, every bottle about contains 35g solid medium, inoculates 5ml liquid cultivation seed；
Q8: production management: the production cycle is about 45 days；
Q81: send out bacterium: postvaccinal cultivation, based in culture bottle, holds with pallet to be placed in and sends out bacterium room, and 2～5 stackings are put, temperature control
System is between 18 DEG C～22 DEG C, and relative air humidity controls between 60%～70%, dark culturing, ventilation, keeps sky
Gas is fresh；
Q82: see that light is cultivated: see light by added for the culture bottle covering with mycelia under the conditions of light culture, Artificial Control indoor epidemic disaster and
Intensity of illumination, temperature is maintained at 18 DEG C-22 DEG C, and humidity is maintained at 60-75%；
Q83: ventilate: keep room air fresh, ventilation of regularly windowing every day；
Q84: miscellaneous bacteria, pest control:
Q841: physical control: regulate cultivation indoor temperature and humidity in production process, it is to avoid overdrying or humidity, adds forced ventilation
Ventilation；
Vent installs gauze and uviol lamp bactericidal unit, and preventing and treating demodicid mite class, insects and miscellaneous bacteria enter；
Remove immediately and autoclaving after finding polluter in workshop, prevent and treat secondary pollution；
Ultraviolet light is used to carry out disinfection in whole culturing room under conditions of without culture；
Q842: chemical prevention: use under conditions of without culture efficiently, low toxicity chemical agent carries out thorough disinfection；
Long more than the 5cm of Q9: gather: sporophore, top forms Sporangium and gathers during without spores flying；Sporophore base is pinned with hands
Portion adopts down, and vessel is advisable with shallow bid, in order to avoid extruding；Manage after adopting: the culture bottle after gathering empties, scrubs sterilizing, repeats profit
With；
Q10: pack, transport: avoid Exposure to Sunlight, drench with rain, prevent from falling hit, weight, forbid with poisonous, be harmful to, have the mixed fortune of abnormal flavour article；
Q11: storage: the Cordyceps militaris after gathering is 50 DEG C of drying in drying baker, water content is less than in the case of 10% 10 DEG C～25
Preserve 12 months at DEG C；Storage house should meet rainproof, protection against rodents, insect protected, ventilate, be dried, clean, shady and cool, without direct sunlight
Requirement, forbid with poisonous, be harmful to, be corrosive, have abnormal flavour, the article that easily pollute are mixed puts.
A kind of Cordyceps militaris industrial production method the most according to claim 1, it is characterised in that the mother in described Q1 step
Plant the prescription separated should meet:
A kind of Cordyceps militaris industrial production method the most according to claim 1 and 2, it is characterised in that in described Q1 step
Female prescription separated of planting should meet:
。
A kind of Cordyceps militaris industrial production method the most according to claim 1, it is characterised in that the system in described Q4 step
The prescription making original seed should meet:
A kind of Cordyceps militaris industrial production method the most according to claim 1, it is characterised in that the liquid in described Q5 step
The organoleptic requirements of body cultigen should meet:
。
A kind of Cordyceps militaris industrial production method the most according to claim 1, it is characterised in that joining in described Q5 step
Put being respectively as follows: of culture medium
Mother plants and Medium for original variety is: Rhizoma Solani tuber osi, glucose, agar culture medium, specifically comprises: Rhizoma Solani tuber osi, glucose, phosphorus
Acid dihydride potassium, magnesium sulfate, vitamin, agar powder, water；
Liquid cultivation seed culture medium prescription is: Rhizoma Solani tuber osi, glucose culture solution, specifically comprises: Rhizoma Solani tuber osi, glucose, di(2-ethylhexyl)phosphate
Hydrogen potassium, magnesium sulfate, vitamin, water；
Culture medium for producing: Semen Tritici aestivi, liquid cultivation seed PDA culture fluid, pH value 6.0～7.0, animal proteinum is Pupa bombycis or chicken
Egg.
A kind of Cordyceps militaris industrial production method the most according to claim 1, it is characterised in that female kind and pedigree seed culture medium
Formula specifically comprises: Rhizoma Solani tuber osi 200g, glucose 20g, potassium dihydrogen phosphate 7.0g, magnesium sulfate 3.5g, vitamin B10.1g, fine jade
Cosmetics 20g, water 1000mL, pH value is 6.0～7.0；Liquid cultivation seed culture medium prescription is particularly as follows: Rhizoma Solani tuber osi 200g, glucose
20g, potassium dihydrogen phosphate 7.0g, magnesium sulfate 3.5g, vitamin B10.1g, water 1000mL, pH value is 6.0～7.0；For producing
The standard culture based formulas of each culture bottle particularly as follows: Semen Tritici aestivi 35g, liquid cultivation seed PDA culture fluid 50mL, pH6.0～
7.0；Animal proteinum 20g.
A kind of Cordyceps militaris industrial production method the most according to claim 1, it is characterised in that described Cordyceps militaris batch production
Production method and technique, its production equipment with production environment is:
Production equipment is: balance, high-pressure sterilizing pot, superclean bench or inoculating hood, calorstat, microscope and alcohol burner；Factory Building
If Feedstock treating room, spice room, subpackage room, sterile chamber, cooling chamber, connect bacterium room, send out bacterium room, culturing room, storeroom, packer's bay；
Factory Building internal medium is provided with district of sterile working, and district of sterile working arranges uviol lamp, and for every day, timing carries out physical disinfection
Sterilizing；Workshop is outside uses warming facility, internal employing air-conditioning temperature control facility, it is ensured that indoor temperature controls to 18 DEG C～22
Between DEG C；
Culturing rack is provided with is satisfied with the light source cultivating sporophore, and intensity of illumination controls between 500LUX～1000LUX；Every
There is special vent in culturing room, filtration wind apparatus, indoor CO need to be installed at vent2Concentration is not higher than 1000PPM.
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