CN108293590A - A kind of cordyceps flower efficient industrial cultural method - Google Patents

A kind of cordyceps flower efficient industrial cultural method Download PDF

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Publication number
CN108293590A
CN108293590A CN201710830508.8A CN201710830508A CN108293590A CN 108293590 A CN108293590 A CN 108293590A CN 201710830508 A CN201710830508 A CN 201710830508A CN 108293590 A CN108293590 A CN 108293590A
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cordyceps flower
compost
culture
cultural method
efficient industrial
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缪建
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Animal Husbandry (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental & Geological Engineering (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Mechanical Engineering (AREA)
  • Food Science & Technology (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention proposes a kind of cordyceps flower efficient industrial cultural method, is related to cordyceps flower technical field of cultivation, solves the problems, such as existing planting technique low output.The cultural method includes step 1, the preparation of compost:Wheat is cleaned into immersion, husk cleaning, rice in steep, corn flour profit water and is mixed, is prepared into compost, natural pH, water content control is 62%~63%;Compost is filled basin, sealer, and sterilized by step 2;Step 3 uniformly sprays cordyceps flower strain on the compost in basin;Step 4, Mycelium culture;Step 5, mycelium annesl;Step 6 goes out careless culture.The cordyceps flower efficient industrial cultural method of the present invention is using addition rice, husk, corn flour in wheat; homogenization liquid strain inoculation technique, three-stage temperature control technique, intermittent LED illumination technology realize cordyceps flower automation, standardization and scale whole year production.

Description

A kind of cordyceps flower efficient industrial cultural method
Technical field
The present invention relates to cordyceps flower technical field of cultivation, and in particular to a kind of cordyceps flower efficient industrial cultural method.
Background technology
Cordyceps flower is a kind of rare traditional Chinese medicine and nourishing food come out at present by artificial cultivation, is the generation of natural cs For product.By detection, medicinal efficacy is almost the same with natural cs, the cordycepic acid and cordycepin ratio contained by cordyceps flower naturally Cordyceps sinensis is taller.Show through modern medicine study:Cordycepin is a kind of novel broad-spectrum antibiotic, is curing the fields such as pulmonary tuberculosis There is uniqueness.It also has the function of anticancer, adjusts human endocrine and enhancing immune function of human body.In addition, science is real To verify bright, in seven big activity of fighting against senium ingredients at present recognized by medical field, cordyceps sinensis also covers five major class, is polysaccharide respectively, Amino acid, protein, nucleic acid and vitamin, flavones and glycoside.Human body can be supplemented since cordyceps flower contains abundant nutriment Needed for growth, therefore liked by people, while also having expedited the emergence of the upsurge of plantation cordyceps flower.But since the cultivation of grasp is planted Method is improper or process control is bad, causes cultivation cycle long, or even mouldy, rotten, rotten show occurs in incubation As giving up the enthusiasm for having accumulated farmer, undoubtedly also increasing planting cost.It is to pursue interests maximum also to have individual farmers Change, do not stint and a large amount of antibiotic are added in planting incubation, medicament residue finally jeopardizes the strong of people on cordyceps flower Health.It is therefore proposed that a kind of green and healthy efficient cordyceps flower implantation methods, it has also become the demand of current plantation cordyceps flower.
Invention content
The present invention provides a kind of cordyceps flower efficient industrial cultural methods of standardization and scale.
Technical solution is used by the present invention realizes above-mentioned technique effect:
A kind of efficient cultivation method of cordyceps flower, includes the following steps:
Step 1, the preparation of compost:Wheat, rice are cleaned into immersion, husk cleaning, corn flour profit water and mixed equal It is even, it is prepared into compost, natural pH, water content control is 65% or so;
Compost is mixed dress basin, epiphragma, and 121 DEG C of sterilizing 2h by step 2 again;
Step 3, uniform sprinkling inoculation cordyceps flower strain in the compost in basin;
Step 4, cultural hypha:It is 16-18 DEG C that the culture medium of inoculation cordyceps flower strain in step 3, which is placed in temperature, Relative air humidity is to cultivate under the conditions of being protected from light of 60%-70%, and waits for the full bacterium bag of mycelia hair;
Step 5, annesl culture:It is 19-23 DEG C that the mycelia obtained in step 4, which is placed in temperature, air humidity 60%- 70% have carries out culture 7-10d under the conditions of light;
Step 6 goes out careless culture:Include the culture of following three phases, fructification shaping stage culture:Temperature control exists It 16-18 DEG C, cultivates 8-10 days;It is artificial to inhibit stage culture:Temperature control is cultivated 8-10 days at 14-16 DEG C;Grow grass the stage culture: 10d is cultivated in temperature control at 19-23 DEG C;Three phases are all made of intermittent photo-irradiation treatment, and air humidity is controlled in 80%- 85%.
Further, the wheat in step 1, rice pre-process before being mixed with unclassified stores by following:To wheat, Rice progress cleaning and dipping is until full penetration, i.e., interior to form water soaking mode without the white heart;The advance that corn flour is mixed with unclassified stores Row profit water process, moistens water in three times, cannot be agglomerating, water suction saturation;Husk is cleaned and is impregnated before being mixed with unclassified stores Processing, washes away dust, suctions moisture.
Further, in step 3, the liquid spawn of homogenization is inoculated in compost after sterilization.
Further, in step 6, three phases are all made of intermittent LED illumination processing, irradiate 2min, whole day 24 per hour Hour accumulative irradiation 48min.Premised on low-carbon energy-saving, the advantage of LED light source is given full play to, creates intermittent light source processing skill Art takes the intermittent LED light source treatment with irradiation using hour as unit in cordyceps flower growth phase, irradiates 2min per hour, for 24 hours The interior cumulative exposure time only needs 48min, misshapen mushroom ratio can be reduced to 1.7%, and increase compared to incandescent light source treatment with irradiation Add yield 5%.Create as a result, low energy consumption, high production, quality product matter cordyceps flower industrial cultivation technique.
Further, in step 6, three cultivation stage temperature use high and low, high method so that cordyceps flower was being developed Cheng Zhong reaches neat and consistent, individual sturdy, the conversion sufficient purpose of nutrition.
Beneficial effects of the present invention are:The present invention cordyceps flower efficient cultivation method using three-stage temperature control technique, Formula of having a rest LED illumination technology, liquid spawn inoculation technique, after-ripening administrative skill etc. can ensure that the stable high yield of factory culture, together When, the technology integrated out has specific working specification and parameter request, energy high-efficiency popularization to realize cordyceps flower automation, standard Change and scale whole year production.The application of the technology can effectively drive the cordyceps flower efficient industrial of other enterprises to produce.Worm Showy flowers of herbaceous plants factory culture, without influence, produces healthy pollution-free food to surrounding enviroment, and a whole set of technology can make full use of abundant husk Resource produces delicious edible mushroom, while abundant town and country vegetable basket, promotes the commodity value of agricultural and sideline product husk.Meanwhile it passing through The quality of pig, ox, chicken, duck etc., ecological benefits can be improved with good feed by crossing the cordyceps flower bacteria residue after edible mushroom bioconversion Clearly.
Specific implementation mode
For a clearer understanding of the technical characteristics, objects and effects of the present invention, now with specific embodiment to this Invention is described in detail.
A kind of embodiment one, efficient cultivation method of cordyceps flower, includes the following steps:
Step 1, the preparation of compost:Wheat 75%, rice 10%, corn flour 5%, husk 10% are pre-processed It is sufficiently mixed, is prepared into compost, natural pH, water content control is 65% or so;Wheat is mixing it with unclassified stores It is preceding to be pre-processed by following:It is to wheat cleaning and dipping until being thoroughly impregnated, i.e., interior to form water soaking mode without the white heart, rice is cleaned and is soaked Bubble is until full penetration, i.e., interior to form water soaking mode without the white heart;Corn flour carries out profit water process before being mixed with unclassified stores, divides three Secondary profit water, cannot be agglomerating, water suction saturation;Husk carries out cleaning and immersion treatment before being mixed with unclassified stores, wash away dust, inhales Sufficient moisture.
Compost is mixed dress basin, epiphragma by step 2 again, and feed thickness 1.5-2cm per basin, fills 121 DEG C of sterilizings after basin 2h。
Sterilizing is cooled to 20 DEG C of composts below by step 3, and the liquid seeds liquid uniformed in sprinkling moves in culture Room light culture.
Step 4, cultural hypha:It is 16-18 DEG C by the inoculum temperature in step 3, relative air humidity 60%- 70% dark place is cultivated under the conditions of being protected from light, and waits for that mycelium covers with 95% or more of full basin;
Step 5, annesl culture:It is 19-23 DEG C that mycelia in step 4, which is transferred to temperature, air humidity 60%-70% Flower bud room, respectively turn on light 5-7h sooner or later daily, mycelium allowed to switch to yellow by white, and carry out vernalization until a large amount of white budlets Occur.
Step 6 goes out careless culture:Include the culture of following three phases, fructification shaping stage culture:Temperature control exists It 16-18 DEG C, cultivates 8-10 days;It is artificial to inhibit stage culture:Temperature control is cultivated 8-10 days at 14-16 DEG C;Grow grass the stage culture: 10d is cultivated in temperature control at 19-23 DEG C;Three phases are all made of intermittent photo-irradiation treatment, and air humidity is controlled in 80%- 85%.Intermittent LED illumination processing method is to irradiate 2min per hour, 24 hours accumulative irradiation 48min of whole day, to obtain height The cordyceps flower of quality.
A kind of embodiment two, efficient cultivation method of cordyceps flower, includes the following steps:
Step 1, the preparation of compost:By wheat 70%, rice 20%, corn flour 5%, husk 5% pre-process into Row is sufficiently mixed, and is prepared into compost, natural pH, water content control is 65% or so;Wheat with unclassified stores before mixing By pre-processing as follows:It is to wheat cleaning and dipping until being thoroughly impregnated, i.e., interior to form water soaking mode without the white heart, to rice cleaning and dipping It is until full penetration, i.e., interior to form water soaking mode without the white heart;Corn flour carries out profit water process before being mixed with unclassified stores, in three times Moisten water, cannot be agglomerating, water suction saturation;Husk carries out cleaning and immersion treatment before being mixed with unclassified stores, wash away dust, suction Moisture.
Compost is mixed dress basin, epiphragma by step 2 again, and feed thickness 1.5-2cm per basin, fills 121 DEG C of sterilizings after basin 2h。
Sterilizing is cooled to 20 DEG C of composts below by step 3, and the liquid seeds liquid uniformed in sprinkling moves in culture Room light culture.
Step 4, cultural hypha:It is 16-18 DEG C by the inoculum temperature in step 3, relative air humidity 60%- 70% dark place is cultivated under the conditions of being protected from light, and waits for that mycelium covers with 95% or more of full basin;
Step 5, annesl culture:It is 19-23 DEG C that mycelia in step 4, which is transferred to temperature, air humidity 60%-70% Flower bud room, respectively turn on light 5-7h sooner or later daily, mycelium allowed to switch to yellow by white, and carry out vernalization until a large amount of white budlets Occur.
Step 6 goes out careless culture:Include the culture of following three phases, fructification shaping stage culture:Temperature control exists It 16-18 DEG C, cultivates 8-10 days;It is artificial to inhibit stage culture:Temperature control is cultivated 8-10 days at 14-16 DEG C;Grow grass the stage culture: 10d is cultivated in temperature control at 19-23 DEG C;Three phases are all made of intermittent photo-irradiation treatment, and air humidity is controlled in 80%- 85%.Intermittent LED illumination processing method is to irradiate 2min per hour, 24 hours accumulative irradiation 48min of whole day, to obtain height The cordyceps flower of quality.
A kind of embodiment three, efficient cultivation method of cordyceps flower, includes the following steps:
Step 1, the preparation of compost:Wheat 15%, rice 60%, corn flour 10%, husk 10% are pre-processed It is sufficiently mixed, is prepared into compost, natural pH, water content control is 65% or so;Wheat is mixing it with unclassified stores It is preceding to be pre-processed by following:It is to wheat cleaning and dipping until being thoroughly impregnated, i.e., interior to form water soaking mode without the white heart, rice is cleaned and is soaked Bubble is until full penetration, i.e., interior to form water soaking mode without the white heart;Corn flour carries out profit water process before being mixed with unclassified stores, divides three Secondary profit water, cannot be agglomerating, water suction saturation;Husk carries out cleaning and immersion treatment before being mixed with unclassified stores, wash away dust, inhales Sufficient moisture.
Compost is mixed dress basin, epiphragma by step 2 again, and feed thickness 1.5-2cm per basin, fills 121 DEG C of sterilizings after basin 2h。
Sterilizing is cooled to 20 DEG C of composts below by step 3, and the liquid seeds liquid uniformed in sprinkling moves in culture Room light culture.
Step 4, cultural hypha:It is 16-18 DEG C by the inoculum temperature in step 3, relative air humidity 60%- 70% dark place is cultivated under the conditions of being protected from light, and waits for that mycelium covers with 95% or more of full basin;
Step 5, annesl culture:It is 19-23 DEG C that mycelia in step 4, which is transferred to temperature, air humidity 60%-70% Flower bud room, respectively turn on light 5-7h sooner or later daily, mycelium allowed to switch to yellow by white, and carry out vernalization until a large amount of white budlets Occur.
Step 6 goes out careless culture:Include the culture of following three phases, fructification shaping stage culture:Temperature control exists It 16-18 DEG C, cultivates 8-10 days;It is artificial to inhibit stage culture:Temperature control is cultivated 8-10 days at 14-16 DEG C;Grow grass the stage culture: 10d is cultivated in temperature control at 19-23 DEG C;Three phases are all made of intermittent photo-irradiation treatment, and air humidity is controlled in 80%- 85%.Intermittent LED illumination processing method is to irradiate 2min per hour, 24 hours accumulative irradiation 48min of whole day, to obtain height The cordyceps flower of quality.
Cordyceps flower one, cordyceps flower two, three yield of cordyceps flower are high, with short production cycle, and abnormal rate is few.
In actual production, by experimental results demonstrate cordyceps flower individual produced by the invention is neat, sturdy, color is golden Huang meets the standard of first-class cordyceps flower.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and what is described in the above embodiment and the description is only the present invention Principle, various changes and improvements may be made to the invention without departing from the spirit and scope of the present invention, these variation and Improvement is both fallen in the range of claimed invention, the present invention claims protection domain by appended claims and its Equivalent defines.

Claims (6)

1. a kind of cordyceps flower efficient industrial cultural method, which is characterized in that include the following steps:
Step 1, the preparation of compost:Wheat, rice are cleaned into immersion, husk cleaning, corn flour profit water and are uniformly mixed, is made For at compost, natural pH, water content control is 65% or so;
Compost is mixed dress basin, epiphragma, and 121 DEG C of sterilizing 2h by step 2 again;
Step 3, the uniform cordyceps flower strain of sprinkling inoculation homogenization in the compost in basin;
Step 4, cultural hypha:It is 16-18 DEG C that the culture medium of inoculation cordyceps flower strain in step 3, which is placed in temperature, air Relative humidity is to cultivate under the conditions of being protected from light of 60%-70%, and waits for the full full basin of mycelium hair;
Step 5, annesl culture:It is 19-23 DEG C that the mycelia obtained in step 4, which is placed in temperature, air humidity 60%-70% Have and carry out culture 7-10d under the conditions of light;
Step 6 goes out careless culture:Include the culture of following three phases, fructification shaping stage culture:Temperature is controlled in 16-18 DEG C, it cultivates 8-10 days;It is artificial to inhibit stage culture:Temperature control is cultivated 8-10 days at 14-16 DEG C;Grow grass the stage culture:Temperature 10d is cultivated in control at 19-23 DEG C;Three phases are all made of intermittent photo-irradiation treatment, and air humidity is controlled in 80%-85%.
2. cordyceps flower efficient industrial cultural method as described in claim 1, it is characterised in that:Wheat in step 1 with By following pretreatment before unclassified stores mixing:It is to wheat cleaning and dipping until full penetration, i.e., interior to form water logging without the white heart Shape, it is to rice cleaning and dipping until full penetration, i.e., interior to form water soaking mode without the white heart;The advance that corn flour is mixed with unclassified stores Row profit water process, moistens water in three times, cannot be agglomerating, water suction saturation;Husk is cleaned and is impregnated before being mixed with unclassified stores Processing, washes away dust, suctions moisture.
3. cordyceps flower efficient industrial cultural method as described in claim 1, it is characterised in that:In step 1, in compost Add rice, corn flour so as to cultivate that material nutrient is balanced, more rationally, and cordyceps flower yield is high, quality is good.
4. cordyceps flower efficient industrial cultural method as described in claim 1, it is characterised in that:In step 1, in compost Add husk and so that compost is more loose ventilative, culture substrate utilize more fully, cordyceps flower yield it is high, at low cost.
5. cordyceps flower efficient industrial cultural method as described in claim 1, it is characterised in that:In step 6, three phases It is all made of intermittent LED illumination processing, irradiates 2min, 24 hours accumulative irradiation 48min of whole day per hour.
6. cordyceps flower efficient industrial cultural method as described in claim 1, it is characterised in that:In step 6, use low Temperature, intermittent warming so that individually individual weight increase, condition are good for cordyceps flower.
CN201710830508.8A 2017-09-15 2017-09-15 A kind of cordyceps flower efficient industrial cultural method Pending CN108293590A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014003932A (en) * 2012-06-22 2014-01-16 Iwate Univ Method of culturing plant worm and culture device used therefor
CN103733875A (en) * 2013-11-08 2014-04-23 吉林省农业科学院 Cordyceps militaris factory production method and process
CN105493888A (en) * 2015-12-10 2016-04-20 何寒 Cordyceps militaris cultured from unhulled rice grains
CN106171523A (en) * 2016-07-26 2016-12-07 河北北尚农业科技有限公司 The breeding method of Cordyceps militaris

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014003932A (en) * 2012-06-22 2014-01-16 Iwate Univ Method of culturing plant worm and culture device used therefor
CN103733875A (en) * 2013-11-08 2014-04-23 吉林省农业科学院 Cordyceps militaris factory production method and process
CN105493888A (en) * 2015-12-10 2016-04-20 何寒 Cordyceps militaris cultured from unhulled rice grains
CN106171523A (en) * 2016-07-26 2016-12-07 河北北尚农业科技有限公司 The breeding method of Cordyceps militaris

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
卢建明等: "蛹虫草人工代料培育技术", 《广东农业科学》 *
薛变丽等: "工厂化栽培北虫草培养基的优化 ", 《食用菌》 *

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Application publication date: 20180720