CN103704533A - Fish feed additive, application method, application and feed containing additive - Google Patents
Fish feed additive, application method, application and feed containing additive Download PDFInfo
- Publication number
- CN103704533A CN103704533A CN201410003050.5A CN201410003050A CN103704533A CN 103704533 A CN103704533 A CN 103704533A CN 201410003050 A CN201410003050 A CN 201410003050A CN 103704533 A CN103704533 A CN 103704533A
- Authority
- CN
- China
- Prior art keywords
- fish
- additive
- feed
- feed additive
- carp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a fish feed additive, an application method, application and a feed containing the additive. The fish feed additive comprises the following components in parts by weight: 1.27-2.19 parts of arginine, 0.95-1.69 parts of isoleucine, 0.3-0.75 part of vitamin K and 0.05-0.2 part of folic acid. According to the invention, by adding the feed additive in the range, the growth and development of the immune organs of fish are promoted, the specific and non-specific immune functions are improved, the capacity of the fish against aeromonas hydrophila infection is enhanced, the immune protection rate is improved, and the death rate under infection is reduced; and moreover, without the substances causing toxic and side effects on the fish body as well as antibiotic substances, the additive avoids pollution and drug resistance.
Description
Technical field
The invention belongs to Fish-water Fish Farming field, be specifically related to feed addictive, the using method of a kind of freshwater fish, the feed of applying and containing this additive.
Background technology
Fishery-ies product has higher nutritive value, and to improving diet structure, human health level is extremely important in raising.Along with health perception strengthens day by day, consumer is more and more higher to consumption demand amount and the quality requirements of fishery-ies product.Carp, the main cultured freshwater fish of grass carp Deng Wei China, account for the 33-40% of fish culture total output.But in recent years, along with cultivation density improves constantly, the reasons such as breeding environment pollution, cause the various fish disease incidences of disease sharply to raise, and wherein bacterial septicemia is one of serious plant disease of carp.
Aeromonas hydrophila is the Main Pathogenic Bacteria of the popular septicemia of carp.This bacterium extensively distributes in fresh water, mud, soil and human feces, is a kind of typical human-beast-fish ill cause of disease altogether.Aquatic livestock, livestock and poultry and the mankind are all had pathogenic, can cause the septicemia of multiple freshwater fish and mankind's diarrhoea.ASSOCIATE STATISTICS shows: this bacterium is to fresh-water fishes infection rate up to more than 90%, and the death rate, up to more than 65%, causes the economic loss of freshwater aquiculture up to tens billion of units every year.Visible, effectively prevention and control infected by Aeromonas hydrophila is of crucial importance to ensureing the sound development of carp culture industry.
At present, in production, mainly with antibiotic and disinfectant, prevent and treat the fish disease of infected by Aeromonas hydrophila, because it is long-term, uses and caused serious consequence: (1) abuse of antibiotics causes the germ resistance to the action of a drug to strengthen, make fish disease treatment difficulty all the more; (2) disinfectant toxic and side effect is large, and local excitation is strong, carcinogenic, causes the fish stress be serious; (3) destroy breeding water body, contaminant water environment, reduces fish body immunity; (4) medicament residue increases, and Safety of Aquatic Products causes anxiety, Quality Down, and harm humans is healthy.
Summary of the invention
Technical problem to be solved by this invention is to provide and a kind ofly can effectively improves anti-infected by Aeromonas hydrophila ability, and has no side effect, pollution-free, the fish feed additive having no drug resistance and the feed that contains this additive.
In addition, the present invention also provides a kind of using method and application of above-mentioned additive.
The present invention addresses the above problem adopted technical scheme, and a kind of fish feed additive is provided, and comprises the component of following weight portion: arginine 1.27-2.19 part, isoleucine 0.95-1.69 part, vitamin K 0.3-0.75 part, folic acid 0.05-0.2 part.
A fish feed additive, is comprised of the component of following weight portion: arginine 1.27-2.19 part, isoleucine 0.95-1.69 part, vitamin K 0.3-0.75 part, folic acid 0.05-0.2 part.
Preferably, in described fish feed additive, the weight portion of each component is: arginine 1.32-2.14 part, isoleucine 1.01-1.63 part, vitamin K 0.35-0.68 part, folic acid 0.1-0.16 part.
Wherein, described arginine can promote growing of the immune organs such as the spleen of fish and head-kidney, improve quantity of leucocyte, strengthen leukocytes phagocytic ability, promote that the characteristic antibody IgM of fish is synthetic, to strengthen specificity and non-specific immunity and the disease resistance of fish, ensure that fish body is healthy, reduce the incidence of disease and dosage.
Wherein, described isoleucine can promote the head-kidney of fish to grow, strengthen head-kidney oxidation resistance, improve quantity of leucocyte, strengthen leukocytes phagocytic ability, promote the antibody IgM of the anti-Aeromonas hydrophila of fish to synthesize, to improve congenital immunity ability and acquired immunity ability, ensure that fish body is healthy, reduce the incidence of disease and dosage.
Wherein, described vitamin K is the important factor that guarantees the quick aggegation of blood, and the quick aggegation of blood is extremely important for the fish that live in water.Vitamin K can improve enzyme and the non-enzymatic oxidation resistance of fish immune organ simultaneously, guarantees that head-kidney and Splenic structure integrality and function are normal.Improve quantity of leucocyte and phagocytic activity, to strengthen the ability of immunity of organisms and opposing courses of infection, ensure that fish body is healthy, reduce the incidence of disease and dosage.
Wherein, described folic acid is the essential water soluble vitamin of fish, with the form participation one carbon unit transfer of tetrahydrofolic acid, participates in the synthetic and amino acid metabolism of protein, nucleic acid.Suitable folic acid improves the head-kidney of fish and the oxidation resistance of spleen, promotes head-kidney and spleen to grow, and improves quantity of leucocyte, strengthens leukocytes phagocytic ability, to strengthen the ability of fish body opposing courses of infection, reduces the incidence of disease and dosage.
The applicant finds through many experiments, the feed addictive of scope of the present invention can improve the oxidation resistance of fish immune organ head-kidney and spleen, promote head-kidney and spleen to grow, improve quantity of leucocyte, strengthen leukocytes phagocytic ability, to improve specificity and non-specific immunity, improve the anti-infected by Aeromonas hydrophila ability of carp, ensure that fish body is healthy, reduce the incidence of disease and dosage.
Feed addictive of the present invention can reduce the death rate of fish more than 28%, improves immune protective rate more than 43%, improves head-kidney body index more than 21%; Improve spleen body index more than 27%; Improve serum IgM content more than 22.5%; Improve quantity of leucocyte more than 24%; Improve leukocytes phagocytic rate more than 31%.
A using method for described fish feed additive, comprises the steps:
Described fish feed additive is joined in basal feed.
Wherein, the weight ratio of described fish feed additive and basal feed is 1:49-1:24.
Fish feed additive of the present invention and the feed that contains this additive are applicable to the cyprinid fish of freshwater aquiculture, are particularly useful for carp, grass carp.
A kind of feed that contains described fish feed additive.
Wherein, in the feed of described 100 weight portions, the fish feed additive that contains 2-4%.
In sum; the invention has the beneficial effects as follows: by adding the feed addictive of scope of the present invention; promote fish immune organ to grow; improve specificity and non-specific immune function, thereby strengthen the ability of the anti-infected by Aeromonas hydrophila of fish, improve immune protective rate; reduce and infect the death rate; and in this additive, do not contain material and antibiotic substance to fish body toxic side effect, pollution-free, have no drug resistance.
Accompanying drawing explanation
Fig. 1 be add and not interpolation group in the cylindricality contrast figure of the death rate and immune protective rate after carp infected by Aeromonas hydrophila;
Fig. 2 be add and not interpolation group in the cylindricality contrast figure of carp head-kidney and spleen body index;
In figure, A is not interpolation group; B is interpolation group.
The specific embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is done to detailed description further, but embodiments of the present invention are not limited to this.
Embodiment 1:
Get arginine 1.8 weight portions, isoleucine 1.2 weight portions, vitamin K 0.48 weight portion, folic acid 0.12 weight portion, makes feed addictive Q1 after mixing.
In order to verify effect of the present invention, feed addictive of the present invention is applied in the carp upper (body weight is in 6g left and right) of Sichuan, Chongqing, 6 plants in Guangxi and tests.Carp is divided into interpolation group and two groups of not interpolation group, and the daily ration of interpolation group adds above-mentioned feed addictive Q1 according to 1% of fish meal gross weight; The daily ration of not interpolation group is not for adding the fish meal of the identical weight of additive Q1; Two groups of fishes are thrown something and fed 6 times every day, and day feeding rate 5-6%, carries out infection experiment after feeding 53 days.Interpolation and not interpolation group are all injected Aeromonas hydrophila, every group of 3 repetitions, and each repeats 40 tail fishes, Continuous Observation 7 days, the death rate of statistics interpolation group and not interpolation group, measures related immune index, records and be depicted as relevant column diagram.
The test fish that infects Aeromonas hydrophila in above-mentioned test there will be typical hueppe's disease symptom: the long-pending faint yellow or red ascites of body colour blackout, abdominal distention, abdominal cavity, orbital hemorrhage, enteron aisle are congested red and swollen.From the interpolation group shown in table 1 and Fig. 1 and not interpolation group, in the infection death rate of carp and immune protective rate, can find out: more not interpolation group of the interpolation group carp carp death rate reduces by 28%, immune protective rate improves 43%.Therefore, fish feed additive of the present invention and the feed that contains this additive all can significantly improve the ability of the anti-infected by Aeromonas hydrophila of carp, significantly reduce carp and infect the death rate after Aeromonas hydrophila.
Table 1
| ? | Not interpolation group | Interpolation group |
| Carp quantity (tail) | 720 | 720 |
| Infect dead carp quantity (tail) | 432 | 309 |
| Infect the death rate (%) | 60 | 43 |
| Immune protective rate (%) | 40 | 57 |
Immune organ is the place of immunocyte generation, propagation, differentiation, maturation, and it is the normal important guarantee of Fish Immunity that its normal growth is grown.And head-kidney and the spleen important immune organ that is fish.Get the fish of institute's number of columns in table 2, carp head-kidney body exponential sum spleen body index calculate its mean value and be recorded in table 2 in test interpolation group and not interpolation group.From table 2 and Fig. 2, can find out: head-kidney body index and the spleen body index of more not interpolation group of interpolation group carp carp are all significantly increased.Therefore, fish feed additive of the present invention and the feed that contains this additive all effectively promote head-kidney and spleen growth, strengthen carp immunologic function.
Table 2
| ? | Not interpolation group | Interpolation group |
| Sampling fish quantity (tail) | 720 | 720 |
| Head-kidney body index (%) | 0.18 | 0.22 |
| Spleen body index (%) | 0.15 | 0.19 |
Immunoglobulin IgM is the main immune molecule of fish specific humoral immune response, is the important sign of body specific immune function.Test the Immunoglobulins in Serum of the carp of metainfective interpolation group and not interpolation group, the serum IgM content average out to 98mg/L of every tail carp is organized in interpolation, and serum IgM content average out to 80 mg/L of every tail carp are not organized in interpolation.Therefore, fish feed additive of the present invention and the feed that contains this additive all can significantly improve carp immune globulin IgM content, strengthen its specific immune function.
Leucocyte can be engulfed and kill pathogen, and quantity of leucocyte and phagocytic activity are the phylactic important parameters of fish nospecific immunity.Get the fish of institute's number of columns in table 3, carp quantity of leucocyte and phagocytic rate calculate its mean value and be recorded in table 3 in test interpolation group and not interpolation group, can find out: more not interpolation group of interpolation group quantity of leucocyte and leukocytes phagocytic rate are all significantly improved.Therefore, fish feed additive of the present invention and the feed that contains this additive all can significantly improve carp quantity of leucocyte and leukocytes phagocytic rate, strengthen phagocytic activity of leukocytes, improve carp non-specific immunity.
Table 3
| ? | Not interpolation group | Interpolation group |
| Sampling fish quantity (tail) | 720 | 720 |
| Quantity of leucocyte (10 12/L） | 2.70 | 4.57 |
| Leukocytes phagocytic rate (%) | 33 | 45 |
In sum; fish feed additive of the present invention and the feed that contains this additive all can promote fish immunity organ growth to grow; improve specificity and non-specific immunity; thereby strengthen the ability of the anti-infected by Aeromonas hydrophila of fish; improve immune protective rate; reduce and infect the death rate, reduce drug use amount, to realize healthy fish cultivation.
Embodiment 2:
Get arginase 12 .19 weight portion, isoleucine 1.69 weight portions, vitamin K 0.3 weight portion, folic acid 0.05 weight portion, makes feed addictive Q2 after mixing.
Embodiment 3:
Get arginine 1.27 weight portions, isoleucine 0.95 weight portion, vitamin K 0.75 weight portion, folic acid 0.2 weight portion, makes feed addictive Q3 after mixing.
Above-described embodiment 2 and embodiment 3, in the mode identical with embodiment 1, interpolation group is set and not interpolation group is tested.In embodiment 2, the death rate of more not interpolation group of interpolation group carp carp reduces by 33%, and immune protective rate improves 57%; Head-kidney body index improves 21%; Spleen body index improves 29%; Serum IgM content improves 28%; Quantity of leucocyte improves 24%; Leukocytes phagocytic rate improves 31%.In embodiment 3, the death rate of more not interpolation group of interpolation group carp carp reduces by 29%, and immune protective rate improves 68%; Head-kidney body index improves 24%; Spleen body index improves 38%; Serum IgM content improves 23%; Quantity of leucocyte improves 30%; Leukocytes phagocytic rate improves 32%.
Above-described embodiment 2 and embodiment 3 all can obtain following beneficial effect: the feed addictive that adds scope of the present invention; can effectively promote the growth of the immune organ such as head-kidney, spleen of carp; improve specificity and non-specific immune function; thereby strengthen the anti-infected by Aeromonas hydrophila ability of fish; improve immune protective rate, reduce and infect the death rate.
In the mode identical with embodiment 1, other freshwater fishes are carried out to identical test, all can obtain above-mentioned beneficial effect, obviously improve its anti-infected by Aeromonas hydrophila ability.
Claims (7)
1. a fish feed additive, comprises the component of following weight portion: arginine 1.27-2.19 part, isoleucine 0.95-1.69 part, vitamin K 0.3-0.75 part, folic acid 0.05-0.2 part.
2. a fish feed additive, is comprised of the component of following weight portion: arginine 1.27-2.19 part, isoleucine 0.95-1.69 part, vitamin K 0.3-0.75 part, folic acid 0.05-0.2 part.
3. a using method for fish feed additive as claimed in claim 1 or 2, is characterized in that, comprises the steps: described fish feed additive to join in basal feed.
4. the using method of fish feed additive according to claim 3, is characterized in that, the weight ratio of described fish feed additive and basal feed is 1:49-1:24.
5. an application for fish feed additive as claimed in claim 1 or 2, is characterized in that, described fish feed additive is applied to cyprinid fish.
6. a feed that contains fish feed additive as claimed in claim 1 or 2.
7. feed according to claim 6, is characterized in that, in the feed of described 100 weight portions, and the fish feed additive that contains 2-4%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410003050.5A CN103704533B (en) | 2014-01-03 | 2014-01-03 | Fish meal nutrition prescription, using method, application and the feed containing this nutrition prescription |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410003050.5A CN103704533B (en) | 2014-01-03 | 2014-01-03 | Fish meal nutrition prescription, using method, application and the feed containing this nutrition prescription |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103704533A true CN103704533A (en) | 2014-04-09 |
| CN103704533B CN103704533B (en) | 2016-04-27 |
Family
ID=50398206
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410003050.5A Active CN103704533B (en) | 2014-01-03 | 2014-01-03 | Fish meal nutrition prescription, using method, application and the feed containing this nutrition prescription |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103704533B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104799111A (en) * | 2015-05-11 | 2015-07-29 | 四川农业大学 | Nutrition composition capable of enhancing ammonia nitrogen stress resistance of gills and feed |
| CN106306587A (en) * | 2016-08-23 | 2017-01-11 | 四川农业大学 | Nutritional composition capable of alleviating gossypol-induced fish enteritis, application method, application and feed |
| CN106508753A (en) * | 2016-10-13 | 2017-03-22 | 合肥润雨农业科技有限公司 | Treatment method for reducing content of chloromycetin in fish bodies |
| CN114711345A (en) * | 2022-05-06 | 2022-07-08 | 通威股份有限公司 | Chinese herbal medicine additive for preventing and treating bacterial rot disease of alepidote fish and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101297679A (en) * | 2007-08-07 | 2008-11-05 | 天津生机集团有限公司 | Feedstuff additive for improving immunity of livestock and poultry and preparation thereof |
| CN101664107A (en) * | 2008-09-04 | 2010-03-10 | 黄翔鹄 | High-efficiency shrimp immunomodifier |
| CN103039724A (en) * | 2012-11-30 | 2013-04-17 | 青岛玛斯特生物技术有限公司 | Vitamin feed additive premix for sea fish |
-
2014
- 2014-01-03 CN CN201410003050.5A patent/CN103704533B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101297679A (en) * | 2007-08-07 | 2008-11-05 | 天津生机集团有限公司 | Feedstuff additive for improving immunity of livestock and poultry and preparation thereof |
| CN101664107A (en) * | 2008-09-04 | 2010-03-10 | 黄翔鹄 | High-efficiency shrimp immunomodifier |
| CN103039724A (en) * | 2012-11-30 | 2013-04-17 | 青岛玛斯特生物技术有限公司 | Vitamin feed additive premix for sea fish |
Non-Patent Citations (3)
| Title |
|---|
| JUAN ZHAO，等: "Effects of dietary isoleucine on the immune response, antioxidantstatus and gene expression in the head kidney of juvenile Jian carp", 《FISH & SHELLFISH IMMUNOLOGY》 * |
| 封福鲜，等: "精氨酸和赖氨酸对瓦氏黄颡鱼幼鱼生长和非特异性免疫力的影响", 《水产学报》 * |
| 李思明，等: "维生素E、K对动物基因表达的影响", 《中国饲料》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104799111A (en) * | 2015-05-11 | 2015-07-29 | 四川农业大学 | Nutrition composition capable of enhancing ammonia nitrogen stress resistance of gills and feed |
| CN106306587A (en) * | 2016-08-23 | 2017-01-11 | 四川农业大学 | Nutritional composition capable of alleviating gossypol-induced fish enteritis, application method, application and feed |
| CN106508753A (en) * | 2016-10-13 | 2017-03-22 | 合肥润雨农业科技有限公司 | Treatment method for reducing content of chloromycetin in fish bodies |
| CN114711345A (en) * | 2022-05-06 | 2022-07-08 | 通威股份有限公司 | Chinese herbal medicine additive for preventing and treating bacterial rot disease of alepidote fish and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103704533B (en) | 2016-04-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103734550B (en) | A kind of nursery pig concentrated feed | |
| KR101087019B1 (en) | Functional feed additives of marine fisheries and feeds for marine fisheries using thereof | |
| CN103385381B (en) | Special compound feed for litopenaeus vannamei | |
| CN102963985B (en) | Biological preparation for balancing water quality for aquaculture | |
| CN103704533B (en) | Fish meal nutrition prescription, using method, application and the feed containing this nutrition prescription | |
| CN105767571A (en) | Chlorella laying-chicken feed and feeding method thereof | |
| CN103667140B (en) | A kind of compound synergic method of enterococcus faecalis and application thereof | |
| CN104621357A (en) | Novel liquid feed additive and preparation method thereof | |
| CN101999335A (en) | Method for breeding lucid ganoderma pig | |
| CN102090514B (en) | Compound immunopotentiator for flounders and preparation method thereof | |
| CN105638563A (en) | Beef cattle daily ration premixture with five-grain baijiu vinasse and feeding method | |
| CN109874920A (en) | A kind of composite microbial feed additive and preparation method thereof | |
| CN102450409A (en) | Feed additive for raising survival rate of cultured fish and shrimp | |
| CN103315151A (en) | Special feed additive for South American white shrimp | |
| CN106070051A (en) | A kind of ecological cultivation method raising goose scattered | |
| CN1321173C (en) | Bacillus subtilis preparation | |
| JP2018509161A (en) | Archaea in bioactive animal feed, method for producing the composition, and method using the composition | |
| CN103704535B (en) | A kind of fish feed additive, using method, application and the feed containing this additive | |
| CN107373164A (en) | A kind of chlorella broiler fodder and feeding method | |
| CN103750061A (en) | Low fish meal mixed feed for crucian carp | |
| CN105360716A (en) | Feed additive adjusting trionyx sinensis body color | |
| CN105052787A (en) | Coregonus peled fry breeding method | |
| CN107960543A (en) | A kind of suckling piglet glycine type additive premix | |
| CN107751645A (en) | A kind of nutritious cray feed | |
| CN110521642B (en) | Grass carp, crucian carp and bighead carp mixed culture method for mainly culturing grass carps |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190701 Address after: 611130 Yongquan Co-cultivation Industrial Park, Wenjiang District, Chengdu City, Sichuan Province Patentee after: CHENGDU KEFEI FEED TECHNOLOGY CO., LTD. Address before: 610000 No. 211 Huimin Road, Wenjiang District, Chengdu City, Sichuan Province Patentee before: Sichuan Agricultural University |