CN103704535B - A kind of fish feed additive, using method, application and the feed containing this additive - Google Patents
A kind of fish feed additive, using method, application and the feed containing this additive Download PDFInfo
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- CN103704535B CN103704535B CN201410003809.XA CN201410003809A CN103704535B CN 103704535 B CN103704535 B CN 103704535B CN 201410003809 A CN201410003809 A CN 201410003809A CN 103704535 B CN103704535 B CN 103704535B
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- 239000003674 animal food additive Substances 0.000 title claims abstract description 22
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- 230000000996 additive effect Effects 0.000 title abstract description 15
- 238000000034 method Methods 0.000 title abstract description 6
- 241000607528 Aeromonas hydrophila Species 0.000 claims abstract description 20
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- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229910052802 copper Inorganic materials 0.000 claims abstract description 10
- 239000010949 copper Substances 0.000 claims abstract description 10
- 239000011701 zinc Substances 0.000 claims abstract description 10
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 10
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229910052748 manganese Inorganic materials 0.000 claims abstract description 9
- 239000011572 manganese Substances 0.000 claims abstract description 9
- 241000252233 Cyprinus carpio Species 0.000 claims description 36
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- 101710186708 Agglutinin Proteins 0.000 description 12
- 101710146024 Horcolin Proteins 0.000 description 12
- 101710189395 Lectin Proteins 0.000 description 12
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- 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 12
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 12
- 108010028780 Complement C3 Proteins 0.000 description 10
- 102000016918 Complement C3 Human genes 0.000 description 10
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- 235000019733 Fish meal Nutrition 0.000 description 2
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- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
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- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a kind of fish feed additive, using method, application and the feed containing this additive.Described fish feed additive comprises the component of following weight portion: copper 0.057-0.075 part, zinc 0.88-1.14 part, manganese 0.22-0.36 part, zytase 0.008-0.013 part.The present invention is by adding the feed addictive of described scope; improve fish specificity and non-specific immune function; thus strengthen the ability of its anti-infected by Aeromonas hydrophila; improve immune protective rate; reduce infectious age; and the material do not contained in this additive fish body toxic side effect and antibiotic substance, pollution-free, have no drug resistance.
Description
Technical field
The invention belongs to Fish-water Fish Farming field, be specifically related to the feed addictive of a kind of freshwater fish, using method, application and the feed containing this additive.
Background technology
Fishery-ies product has higher nutritive value, and to improving diet structure, human health level is extremely important in raising.Along with health perception strengthens day by day, consumer to the consumption demand amount of fishery-ies product and quality requirements more and more higher.Carp is the main cultured freshwater fish of China, accounts for the 18-20% of fish culture total output.But in recent years, along with cultivation density improves constantly, the reasons such as breeding environment pollution, cause the various fish disease incidence of disease sharply to raise, wherein bacterial septicemia is one of serious plant disease of carp.
Aeromonas hydrophila is the Main Pathogenic Bacteria of the popular septicemia of carp.This bacterium is prevalent in fresh water, mud, soil and human feces, all have pathogenic to aquatic livestock, livestock and poultry and the mankind, be a kind of typical human-beast-fish ill cause of disease altogether, septicemia and mankind's diarrhoea of multiple freshwater fish can be caused, often cause heavy economic loss to freshwater aquaculture industry.ASSOCIATE STATISTICS shows: this bacterium is to fresh-water fishes infection rate up to more than 90%, and the death rate, up to more than 65%, causes the economic loss of freshwater aquiculture up to tens billion of unit every year.Visible, effective prevention and control cultivation carp infected by Aeromonas hydrophila is to ensureing that the sound development of carp culture industry is extremely important.
At present, the antibiotic that the carp disease that control Aeromonas hydrophila causes mainly adopts and disinfectant, because serious problem appears in Long-Time Service: (1) antibiotic abuse causes the germ resistance to the action of a drug to strengthen, make fish disease treat difficulty all the more; (2) chemosterilant toxic and side effect is large, and local excitation is strong, carcinogenic; (3) pollute destruction cultivation water environment, reduce fish body immunity; (4) medicament residue is serious, and aquatic product quality declines, and is detrimental to health.
Summary of the invention
Technical problem to be solved by this invention is to provide one effectively can improve anti-infected by Aeromonas hydrophila ability, and has no side effect, pollution-free, the fish feed additive had no drug resistance and the feed containing this additive.
In addition, the present invention also provides a kind of using method and application of above-mentioned additive.
The present invention's adopted technical scheme that solves the problem provides a kind of fish feed additive, comprises the component of following weight portion: copper 0.057-0.075 part, zinc 0.88-1.14 part, manganese 0.22-0.36 part, zytase 0.008-0.013 part.
A kind of fish feed additive, is made up of the component of following weight portion: copper 0.057-0.075 part, zinc 0.88-1.14 part, manganese 0.22-0.36 part, zytase 0.008-0.013 part.
Preferably, in described fish feed additive, the weight portion of each component is: copper 0.062-0.070 part, zinc 0.93-1.09 part, manganese 0.27-0.31 part, zytase 0.01-0.012 part.
Wherein, described copper is the required micronutrient of fish, appropriate copper can improve the oxidation resistance of fish head-kidney and spleen, Promote immunity organ growth is grown, improve Serum specific antibody IgM, Complement C_3 and C4 content, improve agglutinin and tire, to strengthen fish body specificity and non-specific immunity, opposing pathogenic bacterial infection, reduces the incidence of disease and dosage.
Wherein, described zinc is the required micronutrient of fish, is the activator of numerous metabolic enzyme in fish body.Appropriate zinc can promote that fish immune organ grows, and improves Serum specific antibody IgM, Complement C_3 and C4 content, improves agglutinin and tires, and to strengthen the ability of fish opposing infected by Aeromonas hydrophila, reduces the incidence of disease, reduces dosage.
Wherein, described manganese is the required micronutrient of fish, is constituent and the activator of numerous metabolic enzyme in fish body, participates in carbohydrate, lipid and protein metabolism.Appropriate manganese can improve Serum specific antibody IgM, Complement C_3 and C4 content, improves agglutinin and tires, strengthen the ability of the anti-infected by Aeromonas hydrophila of fish, reduce the incidence of disease and dosage.
Wherein, described zytase effectively can eliminate the destruction of plant feed cell wall substance to the intestinal mucosa structural intergrity of fish, ensures that the gut barrier function of fish is normal, safeguard intestinal health, reduce germ and enter fish body by enteron aisle, ensure that fish body is healthy, reduce the incidence of disease, less dosage.
The applicant finds through many experiments, the feed addictive of scope of the present invention can promote that fish immune organ grows, improve Serum specific antibody IgM, Complement C_3 and C4 content, improve agglutinin to tire, to improve specificity and non-specific immunity, strengthen the anti-infected by Aeromonas hydrophila ability of fish, ensure that fish body is healthy, reduce the incidence of disease, reduce dosage.
Feed addictive of the present invention can reduce the death rate more than 60% of fish, improves immune protective rate more than 68%, improves IgM content more than 21%; Improve content of complement C 3 more than 9%; Improve Complement C4 content more than 12%; Improve agglutinin and tire more than 97%.
A using method for described fish feed additive, comprises the steps:
Described fish feed additive is joined in basal feed.
Wherein, the weight ratio of described fish feed additive and basal feed is 1:99-1:999.
Fish feed additive of the present invention and the feed containing this additive are applicable to the cyprinid fish of freshwater aquiculture, are particularly useful for carp, grass carp.
A kind of feed containing described fish feed additive.
Wherein, in the feed of described 100 weight portions, the fish feed additive containing 0.1-1%.
In sum; the invention has the beneficial effects as follows: by adding the feed addictive of scope of the present invention; promote that fish immune organ grows; improve specificity and non-specific immune function, thus strengthen the anti-infected by Aeromonas hydrophila ability of fish, improve immune protective rate; reduce infectious age; and the material do not contained in this additive fish body toxic side effect and antibiotic substance, pollution-free, have no drug resistance.
Detailed description of the invention
Below in conjunction with embodiment, to the detailed description further of the present invention's do, but embodiments of the present invention are not limited thereto.
Embodiment 1:
Get copper 0.075 part, 0.88 part, zinc, 0.36 part, manganese, zytase 0.012 part, obtained feed addictive A1 after mixing.
In order to verify effect of the present invention, feed addictive of the present invention be applied in Sichuan, Fujian, 7 fishing grounds, Guangdong carp upper (body weight is at about 7g) test.Carp is divided into experimental group and control group two groups, the daily ration of experimental group adds above-mentioned feed addictive A1 according to 0.5% of fish meal gross weight; The daily ration of control group is the fish meal of the identical weight of not adding additive A 1; Two groups of fishes are thrown something and fed 6 times every day, and day feeding rate 5-6%, carries out infection experiment after feeding 70 days.Experimental group and control group all arrange injection Aeromonas hydrophila, and often organize 3 repetitions, each repetition 55 tail fish, Continuous Observation 7 days, the death rate of statistical experiment group and control group, measures and record related immune index.
The test fish infecting Aeromonas hydrophila in above-mentioned test there will be typical hueppe's disease symptom: the congested redness of body colour blackout, abdominal distention, seroperitoneum, enteron aisle, spleen and kidney blackening.As can be seen from the infectious age and immune protective rate of the experimental group carp shown in table 1 and control group carp: experimental group carp reduces by 60.3% compared with the death rate of control group carp; immune protective rate improves 68.1%; therefore; fish feed additive of the present invention and the feed containing this additive all can significantly improve the ability of the anti-infected by Aeromonas hydrophila of carp, significantly reduce the death rate after carp infection Aeromonas hydrophila.
Table 1
| Control group | Experimental group | |
| Carp quantity (tail) | 165 | 165 |
| Infect dead carp quantity (tail) | 87 | 35 |
| Infectious age (%) | 53 | 21 |
| Immune protective rate (%) | 47 | 79 |
The Serum of Common Carp immunoglobulin (Ig) result of test experiments group and control group shows: serum IgM content average out to 167 mg/L of every tail carp in experimental group, and serum IgM content average out to 138 mg/L of the every tail carp of control group.Fish feed additive of the present invention and the Feed Energy containing this additive is adopted to make fish after the infection being subject to Aeromonas hydrophila, produce specific immune response, significantly improve the content of the Immunoglobulin IgM in carp body, the invasion and attack of specificity opposing pathogen, safeguard body health.Therefore, fish feed additive of the present invention and the feed containing this additive all can significantly improve the immune globulin IgM content in carp body, strengthen carp specific immunity ability.
Carbohydrate is the constituent of the causal organism cell surfaces such as bacterium, and agglutinin can be combined with these carbohydrates, to identify cause of disease, and with it agglutinating reaction occurs, and is the important nospecific immunity factors of fish.Fish complement can be combined with antibody or extracellular toxin, is beneficial to immune phagocyte and engulfs, dissolves, kills pathogen.Get the fish of institute's number of columns in table 2, the content of complement C 3 of test experiments group and control group carp, Complement C4 content and agglutinin are tired and are calculated its mean value and be recorded in table 2, can find out: experimental group carp comparatively control group Serum of Common Carp Complement C_3, Complement C4 and agglutinin is tired and is significantly increased.Therefore, fish feed additive of the present invention and all can significantly improve carp complement and agglutinin to the identification of cause of disease and phagocytosis, to strengthen the non-special immunocompetence of carp containing the feed of this additive.
Table 2
| Control group | Experimental group | |
| Sampling fish quantity (tail) | 130 | 130 |
| Content of complement C 3 (mg/L) | 93 | 101 |
| Complement C4 content (mg/L) | 25 | 28 |
| Agglutinin is tired (1: X) | 36 | 75 |
In sum; fish feed additive of the present invention and the feed containing this additive all can improve fish specificity and non-specific immune function; thus strengthen the ability of the anti-infected by Aeromonas hydrophila of fish; improve immune protective rate; reduce infectious age; reduce drug use amount, to realize healthy fish cultivation.
Embodiment 2:
Get copper 0.057 weight portion, zinc 1.14 weight portion, manganese 0.31 weight portion, zytase 0.008 weight portion, obtained feed addictive A2 after mixing.
Embodiment 3:
Get copper 0.070 weight portion, zinc 0.93 weight portion, manganese 0.22 weight portion, zytase 0.013 weight portion, obtained feed addictive A3 after mixing.
Above-described embodiment 2 and embodiment 3 arranges experimental group in the same manner as example 1 and control group is tested.In embodiment 2, experimental group carp reduces by 64% compared with the death rate of control group carp, and immune protective rate improves 130%; IgM content improves 37%; Content of complement C 3 improves 26%; Complement C4 content improves 59%; Agglutinin is tired raising 97%.In embodiment 3, experimental group carp reduces by 61% compared with the death rate of control group carp, and immune protective rate improves 93%; IgM content improves 26%; Content of complement C 3 improves 22%; Complement C4 content improves 67%; Agglutinin is tired raising 100%.
Above-described embodiment 2 and embodiment 3 all can obtain following beneficial effect: the feed addictive adding scope of the present invention; can effectively improve carp specificity and non-specific immune function; thus strengthen the ability of the anti-infected by Aeromonas hydrophila of fish; improve immune protective rate; reduce infectious age; reduce drug use amount, to realize healthy fish cultivation.
In the same manner as example 1 identical test is carried out to other freshwater fishes, all can obtain above-mentioned beneficial effect, significantly improve its anti-infected by Aeromonas hydrophila ability.
Claims (3)
1. a carp feed additive special for anti-infected by Aeromonas hydrophila, is made up of the component of following weight portion: copper 0.057-0.075 part, zinc 0.88-1.14 part, manganese 0.22-0.36 part, zytase 0.008-0.013 part.
2. the feed containing, for example the carp feed additive special of anti-infected by Aeromonas hydrophila according to claim 1.
3. feed according to claim 2, is characterized in that, in the feed of described 100 weight portions, and the carp feed additive special of the anti-infected by Aeromonas hydrophila containing 0.1-1%.
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| CN201410003809.XA CN103704535B (en) | 2014-01-03 | 2014-01-03 | A kind of fish feed additive, using method, application and the feed containing this additive |
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| CN104799111A (en) * | 2015-05-11 | 2015-07-29 | 四川农业大学 | Nutrition composition capable of enhancing ammonia nitrogen stress resistance of gills and feed |
| CN110463861A (en) * | 2019-08-13 | 2019-11-19 | 河南科技大学 | A kind of feed additive and preparation method improving fancy carp gut barrier function |
| CN117084324B (en) * | 2023-10-20 | 2024-01-16 | 烟台大学 | Application of icebinding bacteria in Atlantic salmon culture |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102366030A (en) * | 2011-10-25 | 2012-03-07 | 陕西大秦汉集团有限公司 | Mixed composite microbial feed for freshwater fish and production method thereof |
| CN102422978A (en) * | 2011-10-25 | 2012-04-25 | 陕西大秦汉集团有限公司 | Biological mixed feed capable of enhancing quality of animal meat and production technology thereof |
| CN103271249A (en) * | 2013-06-14 | 2013-09-04 | 百洋水产集团股份有限公司 | Health-care fodder for freshwater fish |
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| WO2001089317A2 (en) * | 2000-05-25 | 2001-11-29 | Diversa Corporation | Dietary aids and methods of use thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102366030A (en) * | 2011-10-25 | 2012-03-07 | 陕西大秦汉集团有限公司 | Mixed composite microbial feed for freshwater fish and production method thereof |
| CN102422978A (en) * | 2011-10-25 | 2012-04-25 | 陕西大秦汉集团有限公司 | Biological mixed feed capable of enhancing quality of animal meat and production technology thereof |
| CN103271249A (en) * | 2013-06-14 | 2013-09-04 | 百洋水产集团股份有限公司 | Health-care fodder for freshwater fish |
Non-Patent Citations (2)
| Title |
|---|
| 铜、铁、锰、锌对黄颡鱼生长和生理机能的影响;蒋蓉;《中国优秀硕士学位论文全文数据库农业科技辑》;20061215(第12期);D052-49,11-22,49-51,66 * |
| 饲用木聚糖酶的研究进展及应用;丁强;《中国饲料》;20101231(第3期);第22-25页 * |
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Effective date of registration: 20170511 Address after: Nansha District Fengze road Guangzhou city Guangdong province 510000 No. 106 fifteen X1520 Co-patentee after: GUANGDONG NUTRIERA BIOTECHNOLOGY Co.,Ltd. Patentee after: GUANGDONG LIANHAO GROUP Co.,Ltd. Address before: Nansha District Fengze road Guangzhou city Guangdong province 510000 No. 106 fifteen X1520 Patentee before: GUANGDONG LIANHAO GROUP Co.,Ltd. |
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Denomination of invention: Fish feed additive, use method, application and feed containing the additive Effective date of registration: 20220928 Granted publication date: 20150923 Pledgee: Shanghai Pudong Development Bank Limited by Share Ltd. Guangzhou branch Pledgor: GUANGDONG NUTRIERA BIOTECHNOLOGY Co.,Ltd.|GUANGDONG LIANHAO GROUP Co.,Ltd. Registration number: Y2022980016556 |
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Date of cancellation: 20231212 Granted publication date: 20150923 Pledgee: Shanghai Pudong Development Bank Limited by Share Ltd. Guangzhou branch Pledgor: GUANGDONG LIANHAO GROUP Co.,Ltd.|GUANGDONG NUTRIERA BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022980016556 |
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Denomination of invention: A fish feed additive, method of use, application, and feed containing the additive Effective date of registration: 20231218 Granted publication date: 20150923 Pledgee: Shanghai Pudong Development Bank Limited by Share Ltd. Guangzhou branch Pledgor: GUANGDONG NUTRIERA BIOTECHNOLOGY Co.,Ltd.|GUANGDONG LIANHAO GROUP Co.,Ltd. Registration number: Y2023980072496 |