CN106508753A - Treatment method for reducing content of chloromycetin in fish bodies - Google Patents
Treatment method for reducing content of chloromycetin in fish bodies Download PDFInfo
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- CN106508753A CN106508753A CN201610891297.4A CN201610891297A CN106508753A CN 106508753 A CN106508753 A CN 106508753A CN 201610891297 A CN201610891297 A CN 201610891297A CN 106508753 A CN106508753 A CN 106508753A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to the technical field of aquaculture, in particular to a treatment method for reducing the content of chloromycetin in fish bodies. Fishes reaching the commercial specification are fished, and the fished commercial fishes are subjected to temporary rearing treatment and then appear on the market to be sold. In the temporary rearing process, workers conduct feeding of toxin expelling adsorption fodder every day, and the toxin expelling adsorption fodder is manufactured with sodium bentonite, corncobs, medicine-level polyvinyl alcohol, vegetable sponge extracts and straw mushroom residue extracts. According to the treatment method, the residue amount of the chloromycetin in the fish bodies can be reduced, and the commercial fishes reach the safety edible standard.
Description
Technical field
The present invention relates to technical field of aquaculture, and in particular to the process side of chloromycetin content in a kind of reduction fish body
Method.
Background technology
Chloromycetin (CAP) is a kind of highly effective broad ectrum antibiotic, is the active drug for treating cholera and bloody dysentery, right
Vibrio cholera, colibacillus, Salmonella have strong bactericidal action, so quite being liked by aquaculture family, are usually used in pre-
Anti- and treatment Animal diseases, are used widely in culture fishery,
But chloromycetin has the possibility of mutation and canceration, the hemopoietic system, digestive system to people has serious toxic reaction, it
By approach such as aquatic products disease treatment and preventions, persistent pollutant may be become in enrichment in aquatic products body, by food
Chain works the mischief to human health, at present, the chloromycetin remained in food caused international organization and in the world it is many country,
The chloromycetin used in edible animal and aquatic products industry is forbidden in the great attention in area, Europe, Canada and the U.S. already, while to me
The requirement of state's outlet aquatic products detection limit is also increasingly stricter, and the detection limit that European Union specifies is changed to 1 μ g/ by 10 original μ g/kg
Kg, is down to 0.13 μ g/kg then again, and the detection limit that U.S. FAD specifies also is reduced to 0.13 μ g/kg by 5 original μ g/kg, I
The Ministry of Agriculture of state has also issued the notice for prohibitting the use of chloromycetin in food animal in May, 2002, but cheap has due to no
The succedaneum of effect, use of the chloromycetin in aquaculture still remain incessant after repeated prohibition.
The content of the invention
It is an object of the invention to provide a kind of processing method for reducing chloromycetin content in fish body.
The technical solution used in the present invention is that a kind of processing method for reducing chloromycetin content in fish body, to reaching commodity
The fish of specification is fished for, and to fishing for the marketable fish for obtaining Jing after temporarily supporting and process, then lists and sells, wherein, in temporary rearing process, often
It manually feeds a toxin expelling adsorption feed, is fed in the ratio of 5-8g/kg, i.e., per kg in feed 5-8g toxin expellings absorption raise
Material, feeds between 8-9 points every morning, during temporarily supporting, in addition to a toxin expelling adsorption feed is fed daily, does not feed any feedstuff；
Wherein, toxin expelling adsorption feed is by sodium bentonite 60-70 parts, corn cob 10-15 parts, pharmaceutical grade Polyethylene alcohol 0.05-
0.07 part, chalina extract 3-6 parts, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract 2-5 parts be constitutive material make obtain；
During making, by sodium bentonite, corn cob, pharmaceutical grade Polyethylene alcohol, chalina extract, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract,
Add water so that in mixture the weight ratio of water after 55-58%, high temperature sterilize, inoculation activation after colloid bacillus cereus,
Cultivate 36～48 hours at a temperature of 30～32 DEG C, will be dried after tunning pelletize, obtain toxin expelling absorption of the present invention and raise
Material；
Wherein, when chalina extract makes, chalina extract is crushed, the volumetric concentration for adding 4 times of volumes is 60-70%
Aqueous acetone solution, at 88-95 DEG C extract 2.5-3 hours, by silica gel column chromatography on extract, be 90% with volumetric concentration
Ethanol water eluting, collects eluent, and lyophilization obtains chalina extract；
Wherein, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract is made as raw material with Volvariella volvacea (Bull.Ex Franch.) Singer． slag and is obtained, during making, will be Volvariella volvacea (Bull.Ex Franch.) Singer． ground-slag broken, and add its weight 5-6
Water mixing defibrination again, adds the koning trichoderma of serosity gross weight 0.2-0.3% in serosity, digests 42-46 at 25-27 DEG C
Hour, after ebuillition of heated 2-3 minutes, 50-52 DEG C of insulation 4-6 hour filters to get filtrate, and be concentrated into original volume 1/2 obtains grass
Mushroom slag extract.
Beneficial effect of the present invention is,
（1）The present invention reduces the processing method of chloromycetin content in fish body and feeds toxin expelling adsorption feed by the temporarily foster phase, continuous to raise
Feed 3-5 days, the chloromycetin of fish body accumulation can be promoted to discharge with feces, the residual quantity of fish chloroycetic in body is reduced, reached marketable fish
To safe edible standard；
（2）It is raw material by sodium bentonite, corn cob, pharmaceutical grade Polyethylene alcohol, chalina extract, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract combination
The toxin expelling adsorbent that making is obtained has preferable adsorptivity to chloromycetin；
（3）Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract in raw material composition has strong fragrant and delicate flavour, has preferable attractant to Fish；
（4）Sodium bentonite, corn cob, pharmaceutical grade Polyethylene alcohol, chalina extract, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract combined inoculation colloid
Fermentation of bacillus, can improve the viscosity of raw material components, obtain tunning Jing after granulating and drying, and in water, immersion is little up to 12
Shi Buhui is scattered, dissolves.
Specific embodiment
Embodiment 1, a kind of processing method for reducing chloromycetin content in fish body, the fish to reaching commercial specification are caught
Drag for, to the marketable fish for obtaining being fished for Jing after temporarily supporting and process, then list and sell, wherein, in temporary rearing process, manually feed once daily
Toxin expelling adsorption feed, is fed in the ratio of 5g/kg, i.e., per kg in 5g feedstuffs of the present invention are fed, throw every morning between 8-9 points
Hello, during temporarily supporting, in addition to a toxin expelling adsorption feed is fed daily, do not feed any feedstuff；
Wherein, feedstuff is by 60 parts of sodium bentonite, 10 parts of corn cob, 0.05 part of pharmaceutical grade Polyethylene alcohol, chalina extract 3
Part, 2 parts of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract are that constitutive material making is obtained；
During making, by sodium bentonite, corn cob, pharmaceutical grade Polyethylene alcohol, chalina extract, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract,
Add water so that in mixture, 55%, after high temperature sterilize, the colloid bacillus cereus after inoculation activation, 30 for the weight ratio of water
Cultivate 36 hours at a temperature of DEG C, will be dried after tunning pelletize, obtain toxin expelling adsorption feed of the present invention；
Wherein, when chalina extract makes, chalina extract is crushed, the volumetric concentration for adding 4 times of volumes is the third of 60%
Ketone aqueous solution, extracts 2.5 hours at 88 DEG C, by silica gel column chromatography on extract, with the ethanol water that volumetric concentration is 90%
Eluting, collects eluent, and lyophilization obtains chalina extract；
Wherein, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract is made as raw material with Volvariella volvacea (Bull.Ex Franch.) Singer． slag and is obtained, during making, will be Volvariella volvacea (Bull.Ex Franch.) Singer． ground-slag broken, and add 5 times of its weight
Water mixing defibrination, add the koning trichoderma of serosity gross weight 0.2% in serosity, digest 42 hours at 25 DEG C, ebuillition of heated
After 2 minutes, 50 DEG C are incubated 4 hours, filter to get filtrate, are concentrated into the 1/2 of original volume and obtain Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract.
The intramuscular chloromycetin content of commodity bream is fished for using the confirmation detection of enzyme linked immunological kit ELISA+ gas chromatographs
For 0.34 μ g/kg, the commodity bream after being fished for the process of 1 method of embodiment, after processing 3 days, again using ELISA reagent
The chloromycetin content in fish tissue is fished in the confirmation detection of box ELISA+ gas chromatographs, is as a result 0.012 μ g/kg of chloromycetin；
The chloromycetin in commodity muscles of Pseudosciaena crocea is fished for using the confirmation detection of enzyme linked immunological kit ELISA+ gas chromatographs to contain
Measure as 0.28 μ g/kg, the commodity Carnis Pseudosciaenae after being fished for the process of 1 method of embodiment, after processing 3 days, again using enzyme linked immunological
The chloromycetin content in fish tissue is fished in the confirmation detection of test kit ELISA+ gas chromatographs, is not as a result detected for chloromycetin；
The intramuscular chloromycetin content of commodity Siniperca chuatsi is fished for using the confirmation detection of enzyme linked immunological kit ELISA+ gas chromatographs
For 0.22 μ g/kg, the commodity Siniperca chuatsi after being fished for the process of 1 method of embodiment, after processing 3 days, again using ELISA reagent
The chloromycetin content in fish tissue is fished in the confirmation detection of box ELISA+ gas chromatographs, is not as a result detected for chloromycetin；
Detection is confirmed using enzyme linked immunological kit ELISA+ gas chromatographs and fishes for the chloromycetin content in commodity carassias auratus muscLe
For 0.38 μ g/kg, the commodity Carassius auratuss after being fished for the process of 1 method of embodiment, after processing 3 days, again using ELISA reagent
The chloromycetin content in fish tissue is fished in the confirmation detection of box ELISA+ gas chromatographs, is as a result 0.02 μ g/kg of chloromycetin, procrypsis
Chloromycetin is not detected；
Embodiment 2, a kind of processing method for reducing chloromycetin content in fish body, the fish to reaching commercial specification is fished for, right
The marketable fish that obtains is fished for Jing after temporarily supporting and process, then is listed and is sold, wherein, in temporary rearing process, manually feed a toxin expelling daily
Adsorption feed, is fed in the ratio of 10g/kg, i.e., per kg in 10g feedstuffs of the present invention are fed, throw every morning between 8-9 points
Hello, during temporarily supporting, in addition to a toxin expelling adsorption feed is fed daily, do not feed any feedstuff；
Wherein, feedstuff is by 65 parts of sodium bentonite, 12 parts of corn cob, 0.06 part of pharmaceutical grade Polyethylene alcohol, chalina extract 4
Part, 3 parts of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract are that constitutive material making is obtained；
During making, by sodium bentonite, corn cob, pharmaceutical grade Polyethylene alcohol, chalina extract, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract,
Add water so that in mixture, 56%, after high temperature sterilize, the colloid bacillus cereus after inoculation activation, 31 for the weight ratio of water
Cultivate 42 hours at a temperature of DEG C, will be dried after tunning pelletize, obtain toxin expelling adsorption feed of the present invention；
Wherein, when chalina extract makes, chalina extract is crushed, the volumetric concentration for adding 4 times of volumes is the third of 65%
Ketone aqueous solution, extracts 2.8 hours at 90 DEG C, by silica gel column chromatography on extract, with the ethanol water that volumetric concentration is 90%
Eluting, collects eluent, and lyophilization obtains chalina extract；
Wherein, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract is made as raw material with Volvariella volvacea (Bull.Ex Franch.) Singer． slag and is obtained, during making, will be Volvariella volvacea (Bull.Ex Franch.) Singer． ground-slag broken, and add its weight 5.5
Water mixing defibrination again, adds the koning trichoderma of serosity gross weight 0.5% in serosity, digests 44 hours at 26 DEG C, heating boiling
After rising 2.5 minutes, 51 DEG C are incubated 5 hours, filter to get filtrate, are concentrated into the 1/2 of original volume and obtain Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract.
Embodiment 3, a kind of processing method for reducing chloromycetin content in fish body, the fish to reaching commercial specification is fished for, right
The marketable fish that obtains is fished for Jing after temporarily supporting and process, then is listed and is sold, wherein, in temporary rearing process, manually feed a toxin expelling daily
Adsorption feed, is fed in the ratio of 10g/kg, i.e., per kg in 10g feedstuffs of the present invention are fed, throw every morning between 8-9 points
Hello, during temporarily supporting, in addition to a toxin expelling adsorption feed is fed daily, do not feed any feedstuff；
Wherein, toxin expelling adsorption feed is by 70 parts of sodium bentonite, 15 parts of corn cob, 0.07 part of pharmaceutical grade Polyethylene alcohol, chalina
6 parts of extract, 5 parts of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract are that constitutive material making is obtained；
During making, by sodium bentonite, corn cob, pharmaceutical grade Polyethylene alcohol, chalina extract, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract,
Add water so that in mixture, 58%, after high temperature sterilize, the colloid bacillus cereus after inoculation activation, at 32 DEG C for the weight ratio of water
At a temperature of cultivate 48 hours, will be dried after tunning pelletize, obtain toxin expelling adsorption feed of the present invention；
Wherein, when chalina extract makes, chalina extract is crushed, the volumetric concentration for adding 4 times of volumes is the third of 70%
Ketone aqueous solution, extracts 3 hours at 95 DEG C, by silica gel column chromatography on extract, with the ethanol aqueous wash that volumetric concentration is 90%
It is de-, eluent is collected, lyophilization obtains chalina extract；
Wherein, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract is made as raw material with Volvariella volvacea (Bull.Ex Franch.) Singer． slag and is obtained, during making, will be Volvariella volvacea (Bull.Ex Franch.) Singer． ground-slag broken, and add 6 times of its weight
Water mixing defibrination, add the koning trichoderma of serosity gross weight 0.3% in serosity, digest 46 hours at 27 DEG C, ebuillition of heated
After 3 minutes, 52 DEG C are incubated 6 hours, filter to get filtrate, are concentrated into the 1/2 of original volume and obtain Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract.
Claims (5)
1. it is a kind of reduce fish body in chloromycetin content processing method, the fish to reaching commercial specification is fished for, to fishing for
The marketable fish for arriving is Jing after temporarily supporting and process, then lists and sell, it is characterised in that
In temporary rearing process, a toxin expelling adsorption feed is manually fed daily,
Wherein, toxin expelling adsorption feed is by sodium bentonite 60-70 parts, corn cob 10-15 parts, pharmaceutical grade Polyethylene alcohol 0.05-
0.07 part, chalina extract 3-6 parts, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract 2-5 parts be constitutive material make obtain；
During making, by sodium bentonite, corn cob, pharmaceutical grade Polyethylene alcohol, chalina extract, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract,
Add water so that in mixture the weight ratio of water after 55-58%, high temperature sterilize, inoculation activation after colloid bacillus cereus,
Cultivate 36～48 hours at a temperature of 30～32 DEG C, will be dried after tunning pelletize, obtain toxin expelling absorption of the present invention and raise
Material.
2. it is according to claim 1 it is a kind of reduce fish body in chloromycetin content processing method, it is characterised in that
When chalina extract makes, chalina extract is crushed, the volumetric concentration of 4 times of volumes is added for the acetone of 60-70%
Aqueous solution, extracts 2.5-3 hours, at 88-95 DEG C by silica gel column chromatography on extract, with the ethanol water that volumetric concentration is 90%
Eluant solution, collects eluent, and lyophilization obtains chalina extract.
3. it is according to claim 1 it is a kind of reduce fish body in chloromycetin content processing method, it is characterised in that
Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract is made as raw material with Volvariella volvacea (Bull.Ex Franch.) Singer． slag and is obtained, during making, will be Volvariella volvacea (Bull.Ex Franch.) Singer． ground-slag broken, and add its weight 5-6 times
Water mixing defibrination, adds the koning trichoderma of serosity gross weight 0.2-0.3% in serosity, digests 42-46 hours at 25-27 DEG C
Afterwards, ebuillition of heated 2-3 minutes, 4-6 hours are incubated at 50-52 DEG C, are filtered to get filtrate, be concentrated into original volume 1/3 obtains Volvariella volvacea (Bull.Ex Franch.) Singer．
Slag extract.
4. in a kind of reduction fish body according to claim 1,2 or 3 chloromycetin content processing method, it is characterised in that
Toxin expelling adsorption feed is fed in the ratio of 5-8g/kg every time, and every morning is fed between 8-9 points.
5. in a kind of reduction fish body according to claim 1,2 or 3 chloromycetin content processing method, it is characterised in that
During temporarily supporting, in addition to a toxin expelling adsorption feed is fed daily, any feedstuff is not fed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610891297.4A CN106508753A (en) | 2016-10-13 | 2016-10-13 | Treatment method for reducing content of chloromycetin in fish bodies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610891297.4A CN106508753A (en) | 2016-10-13 | 2016-10-13 | Treatment method for reducing content of chloromycetin in fish bodies |
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| CN106508753A true CN106508753A (en) | 2017-03-22 |
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| CN201610891297.4A Pending CN106508753A (en) | 2016-10-13 | 2016-10-13 | Treatment method for reducing content of chloromycetin in fish bodies |
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