CN106472373A - The processing method of metabolites of nitrofuran content in a kind of reduction fish body - Google Patents

The processing method of metabolites of nitrofuran content in a kind of reduction fish body Download PDF

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Publication number
CN106472373A
CN106472373A CN201610891296.XA CN201610891296A CN106472373A CN 106472373 A CN106472373 A CN 106472373A CN 201610891296 A CN201610891296 A CN 201610891296A CN 106472373 A CN106472373 A CN 106472373A
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fish
nitrofuran
franch
singer
bull
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CN201610891296.XA
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Chinese (zh)
Inventor
吴邦柱
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Hefei Rain Agricultural Technology Co Ltd
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Hefei Rain Agricultural Technology Co Ltd
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Priority to CN201610891296.XA priority Critical patent/CN106472373A/en
Publication of CN106472373A publication Critical patent/CN106472373A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The present invention relates to technical field of aquaculture, specifically related to a kind of processing method reducing metabolites of nitrofuran content in fish body, the fish reaching commercial specification is fished for, to fish for the marketable fish obtaining through temporarily support process after, list again and sell, wherein, in temporary rearing process, manually feed daily and once made for constitutive material and obtained toxin expelling adsorption feed by Amylum Nelumbinis Rhizomatis, magnesium acetate, hydroxycitric acid, Phenylalanine, Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract, processing method of the present invention can reduce the residual quantity of metabolites of nitrofuran in fish body, makes marketable fish reach safe edible standard.

Description

The processing method of metabolites of nitrofuran content in a kind of reduction fish body
Technical field
The present invention relates to technical field of aquaculture is and in particular to metabolites of nitrofuran content in a kind of reduction fish body Processing method.
Background technology
Nitrofuran metabolites are a kind of broad ectrum antibiotic, to most of gram positive bacterias and gram negative bacteria, true The pathogen such as bacterium and protozoon all has killing action, and they act on microbial enzyme system, suppresses S-acetyl-coenzyme-A, disturbs microorganism The metabolism of saccharide, thus playing bacteriostasis, Nitrofuran metabolites were once widely used in poultry and culture fishery, with treat by Enteritis caused by escherichia coli or Salmonella, scabies, red fin fish disease, Peptic Ulcerss etc., due to Nitrofuran metabolites and its generation Thank to thing has carcinogenic, teratogenesis tire side effect to human body, is prohibited animal sources edible in the mankind by most countries at present Use in food, due to not having cheap effective succedaneum, use in aquaculture for the metabolites of nitrofuran remains incessant after repeated prohibition.
Content of the invention
It is an object of the invention to provide a kind of processing method reducing metabolites of nitrofuran content in fish body.
The technical solution used in the present invention is that in a kind of reduction fish body, the processing method of metabolites of nitrofuran content, right The fish reaching commercial specification is fished for, and sells through temporarily supporting after process, then listing to fishing for the marketable fish obtaining, wherein, temporarily supports During, manually feed a toxin expelling adsorption feed daily, fed in the ratio of 6-9g/kg, that is, every kg is in feeding 6-9g Toxin expelling adsorption feed, every morning feeds between 8-9 point, during temporarily supporting, in addition to feeding a toxin expelling adsorption feed daily, does not feed Any feedstuff;
Wherein, toxin expelling adsorption feed is by Amylum Nelumbinis Rhizomatis 80-100 part, magnesium acetate 0.2-0.3 part, hydroxycitric acid 1-2 part, Phenylalanine 0.03-0.05 part, Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract 2-5 part make for constitutive material and obtain;
During making, Amylum Nelumbinis Rhizomatis, magnesium acetate, hydroxycitric acid, Phenylalanine, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract add water so that mixture The weight ratio of middle water, after 55-58%, high temperature sterilize, inoculates the colloid bacillus cereus after activation, at a temperature of 30~32 DEG C Culture 36~48 hours, is dried after tunning pelletize, obtains toxin expelling adsorption feed of the present invention.
Wherein, Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract is made for raw material with Volvariella volvacea (Bull.Ex Franch.) Singer. slag and obtains, during making, will be broken for Volvariella volvacea (Bull.Ex Franch.) Singer. ground-slag, and add its weight 5-6 Water mixing defibrination again, adds the koning trichoderma of serosity gross weight 0.2-0.3% in serosity, digests 42-46 at 25-27 DEG C Hour, after ebuillition of heated 2-3 minute, 50-52 DEG C of insulation 4-6 hour, filter to get filtrate, be concentrated into original volume 1/2 obtains grass Mushroom slag extract.
The present invention has the beneficial effects that,
(1)The processing method that the present invention reduces metabolites of nitrofuran content in fish body is raised by temporarily supporting phase feeding toxin expelling absorption Material, continuous feeding 3-5 days, the metabolites of nitrofuran of fish body accumulation can be promoted to discharge with feces, reduce nitrofuran in fish body The residual quantity of metabolite, makes marketable fish reach safe edible standard;
(2)The toxin expelling absorption obtaining is made by Amylum Nelumbinis Rhizomatis, magnesium acetate, hydroxycitric acid, Phenylalanine, Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract for raw material Agent has preferable adsorptivity to metabolites of nitrofuran;
(3)Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract in raw material composition has strong fragrant and delicate flavour, has preferable attractant to Fish;
(4)Amylum Nelumbinis Rhizomatis, magnesium acetate, hydroxycitric acid, Phenylalanine, the fermentation of Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract combined inoculation colloid bacillus cereus, The viscosity of raw material components can be improved, obtain tunning after granulating and drying, in water soak will not be scattered up to 7 hours, molten Solution.
Specific embodiment
Embodiment 1, a kind of processing method reducing metabolites of nitrofuran content in fish body, to the fish reaching commercial specification Fished for, sell through temporarily supporting after process, then listing to fishing for the marketable fish obtaining, wherein, in temporary rearing process, manually throw daily Feed a toxin expelling adsorption feed, fed in the ratio of 6g/kg, that is, every kg is in feeding 6g feedstuff of the present invention, every morning 8-9 Feed between point, during temporarily supporting, in addition to feeding a toxin expelling adsorption feed daily, do not feed any feedstuff;
Wherein, toxin expelling adsorption feed is by 80 parts of Amylum Nelumbinis Rhizomatis, 0.2 part of magnesium acetate, 1 part of hydroxycitric acid, 0.03 part of Phenylalanine, Volvariella volvacea (Bull.Ex Franch.) Singer. Slag extract 2-5 part makes for constitutive material and obtains;
During making, by Amylum Nelumbinis Rhizomatis, magnesium acetate, hydroxycitric acid, Phenylalanine, Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract extract mixing, add water so that In mixture, the weight of water ratio is 55%, after high temperature sterilize, colloid bacillus cereus after inoculation activation, and train at a temperature of 30 DEG C Support 36 hours, be dried after tunning pelletize, obtain toxin expelling adsorption feed of the present invention;
Wherein, Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract is made for raw material with Volvariella volvacea (Bull.Ex Franch.) Singer. slag and obtains, during making, will be broken for Volvariella volvacea (Bull.Ex Franch.) Singer. ground-slag, and add 5 times of its weight Water mixing defibrination, in serosity add serosity gross weight 0.2% koning trichoderma, at 25 DEG C digest 42 hours, ebuillition of heated After 2 minutes, 50 DEG C are incubated 4 hours, filter to get filtrate, are concentrated into the 1/2 of original volume and obtain Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract.
Fish for the intramuscular metabolites of nitrofuran of commodity Ctenopharyngodon idellus using the detection of high performance liquid chromatography mass spectrometry HPLC/MS method to contain Measure as 0.50 μ g/kg, the commodity Ctenopharyngodon idellus after fishing for are processed with embodiment 1 method, after 3 days, adopt high performance liquid chromatography matter again The metabolites of nitrofuran content in fish tissue is fished in spectrum combination HPLC/MS method detection, and result is metabolites of nitrofuran 0.018 μg/kg;
Metabolites of nitrofuran in commodity muscles of Pseudosciaena crocea is fished for using the detection of high performance liquid chromatography mass spectrometry HPLC/MS method Content is 0.49 μ g/kg, processes the commodity Carnis Pseudosciaenae after fishing for embodiment 1 method, after 3 days, adopts high-efficient liquid phase color again The metabolites of nitrofuran content in fish tissue is fished in spectrum mass spectrometry HPLC/MS method detection, and result is metabolites of nitrofuran Do not detect;
Fish for the intramuscular metabolites of nitrofuran of commodity Siniperca chuatsi using the detection of high performance liquid chromatography mass spectrometry HPLC/MS method to contain Measure as 0.45 μ g/kg, the commodity Siniperca chuatsi after fishing for is processed with embodiment 1 method, after 3 days, adopt high performance liquid chromatography matter again The metabolites of nitrofuran content in fish tissue is fished in spectrum combination HPLC/MS method detection, and result is not examined for metabolites of nitrofuran Measure;
Fish for the metabolites of nitrofuran in commodity carassias auratus muscLe using the detection of high performance liquid chromatography mass spectrometry HPLC/MS method to contain Measure as 0.36 μ g/kg, the commodity Carassius auratuss after fishing for are processed with embodiment 1 method, after 3 days, adopt high performance liquid chromatography matter again The metabolites of nitrofuran content in fish tissue is fished in spectrum combination HPLC/MS method detection, and result is not examined for metabolites of nitrofuran Measure;
Embodiment 2, a kind of processing method reducing metabolites of nitrofuran content in fish body, are carried out to the fish reaching commercial specification Fish for, sell through temporarily supporting after process, then listing to fishing for the marketable fish obtaining, wherein, in temporary rearing process, manually feed one daily Secondary toxin expelling adsorption feed, is fed in the ratio of 7g/kg, and that is, every kg is in feeding 7g feedstuff of the present invention, between every morning 8-9 point Feed, during temporarily supporting, in addition to feeding a toxin expelling adsorption feed daily, do not feed any feedstuff;
Wherein, toxin expelling adsorption feed by 90 parts of Amylum Nelumbinis Rhizomatis, 0.25 part of magnesium acetate, 1.5 parts of hydroxycitric acid, 0.04 part of Phenylalanine, 3 parts of Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract makes for constitutive material and obtains;
During making, Amylum Nelumbinis Rhizomatis, magnesium acetate, hydroxycitric acid, Phenylalanine, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract add water so that mixture The weight of middle water ratio 56%, after high temperature sterilize, colloid bacillus cereus after inoculation activation, at a temperature of 31 DEG C, culture 42 is little When, it is dried after tunning pelletize, obtain toxin expelling adsorption feed of the present invention.
Wherein, Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract is made for raw material with Volvariella volvacea (Bull.Ex Franch.) Singer. slag and obtains, during making, will be broken for Volvariella volvacea (Bull.Ex Franch.) Singer. ground-slag, and add it heavy The water mixing defibrination of 5.5 times of amount, adds the koning trichoderma of serosity gross weight 0.5% in serosity, digests 44 hours at 26 DEG C, After ebuillition of heated 2.5 minutes, 51 DEG C are incubated 5 hours, filter to get filtrate, and are concentrated into the 1/2 of original volume and obtain the extraction of Volvariella volvacea (Bull.Ex Franch.) Singer. slag Thing.
Embodiment 3, a kind of processing method reducing metabolites of nitrofuran content in fish body, to the fish reaching commercial specification Fished for, sell through temporarily supporting after process, then listing to fishing for the marketable fish obtaining, wherein, in temporary rearing process, manually throw daily Feed a toxin expelling adsorption feed, fed in the ratio of 9g/kg, that is, every kg is in feeding 9g feedstuff of the present invention, every morning 8-9 Feed between point, during temporarily supporting, in addition to feeding a toxin expelling adsorption feed daily, do not feed any feedstuff;
Wherein, toxin expelling adsorption feed is by 100 parts of Amylum Nelumbinis Rhizomatis, 0.3 part of magnesium acetate, 2 parts of hydroxycitric acid, 0.05 part of Phenylalanine, grass 5 parts of mushroom slag extract makes for constitutive material and obtains;
During making, Amylum Nelumbinis Rhizomatis, magnesium acetate, hydroxycitric acid, Phenylalanine, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract add water so that mixture The weight of middle water ratio 58%, after high temperature sterilize, colloid bacillus cereus after inoculation activation, cultivate 48 at a temperature of 32 DEG C Hour, it is dried after tunning pelletize, obtain toxin expelling adsorption feed of the present invention.
Wherein, Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract is made for raw material with Volvariella volvacea (Bull.Ex Franch.) Singer. slag and obtains, during making, will be broken for Volvariella volvacea (Bull.Ex Franch.) Singer. ground-slag, and add 6 times of its weight Water mixing defibrination, in serosity add serosity gross weight 0.3% koning trichoderma, at 27 DEG C digest 46 hours, ebuillition of heated After 3 minutes, 52 DEG C are incubated 6 hours, filter to get filtrate, are concentrated into the 1/2 of original volume and obtain Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract.

Claims (4)

1. a kind of processing method reducing metabolites of nitrofuran content in fish body, fishes for the fish reaching commercial specification, To fish for the marketable fish obtaining through temporarily support process after, then list sell it is characterised in that
In temporary rearing process, manually feed a toxin expelling adsorption feed daily,
Wherein, toxin expelling adsorption feed is by Amylum Nelumbinis Rhizomatis 80-100 part, magnesium acetate 0.2-0.3 part, hydroxycitric acid 1-2 part, Phenylalanine 0.03-0.05 part, Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract 2-5 part make for constitutive material and obtain;
During making, Amylum Nelumbinis Rhizomatis, magnesium acetate, hydroxycitric acid, Phenylalanine, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract add water so that mixture The weight ratio of middle water, after 55-58%, high temperature sterilize, inoculates the colloid bacillus cereus after activation, at a temperature of 30~32 DEG C Culture 36~48 hours, is dried after tunning pelletize, obtains toxin expelling adsorption feed of the present invention.
2. a kind of processing method reducing metabolites of nitrofuran content in fish body according to claim 1, its feature exists In,
Volvariella volvacea (Bull.Ex Franch.) Singer. slag extract is made for raw material with Volvariella volvacea (Bull.Ex Franch.) Singer. slag and obtains, during making, will be broken for Volvariella volvacea (Bull.Ex Franch.) Singer. ground-slag, and add its weight 5-6 times Water mixing defibrination, adds the koning trichoderma of serosity gross weight 0.2-0.3% in serosity, digests 42-46 hour at 25-27 DEG C Afterwards, ebuillition of heated 2-3 minute, is incubated 4-6 hour at 50-52 DEG C, filters to get filtrate, be concentrated into original volume 1/3 obtains Volvariella volvacea (Bull.Ex Franch.) Singer. Slag extract.
3. a kind of processing method reducing metabolites of nitrofuran content in fish body according to claim 1 and 2, its feature It is,
Toxin expelling adsorption feed is fed in the ratio of 10-15g/kg every time, and every morning feeds between 8-9 point.
4. a kind of processing method reducing metabolites of nitrofuran content in fish body according to claim 1 and 2, its feature It is,
During temporarily supporting, in addition to feeding a toxin expelling adsorption feed daily, do not feed any feedstuff.
CN201610891296.XA 2016-10-13 2016-10-13 The processing method of metabolites of nitrofuran content in a kind of reduction fish body Pending CN106472373A (en)

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CN108812443A (en) * 2018-04-11 2018-11-16 河源市渔鲜渔业科技有限公司 A kind of adult fish culture method before Tilapia mossambica listing
CN108812444A (en) * 2018-04-11 2018-11-16 河源市渔鲜渔业科技有限公司 A kind of adult fish culture method before catfish listing

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Publication number Priority date Publication date Assignee Title
CN108812443A (en) * 2018-04-11 2018-11-16 河源市渔鲜渔业科技有限公司 A kind of adult fish culture method before Tilapia mossambica listing
CN108812444A (en) * 2018-04-11 2018-11-16 河源市渔鲜渔业科技有限公司 A kind of adult fish culture method before catfish listing

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