CN103689001A - Bactericide containing arbutus litter extract and purpose of bactericide - Google Patents
Bactericide containing arbutus litter extract and purpose of bactericide Download PDFInfo
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- CN103689001A CN103689001A CN201310725507.9A CN201310725507A CN103689001A CN 103689001 A CN103689001 A CN 103689001A CN 201310725507 A CN201310725507 A CN 201310725507A CN 103689001 A CN103689001 A CN 103689001A
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Abstract
A bactericide containing an arbutus litter extract and a purpose of the bactericide belong to the technical field of natural bactericides. The bactericide is characterized in that the bactericide comprises 17-38% of the arbutus litter extract, 2-15% of an assistant and 51-78% of a solvent. The bactericide containing the arbutus litter extract is prepared by a raw material of pure natural arbutus litter and has strong effects of inhibiting and killing bacteria. The bactericide has strong effect of killing botrytis cinerea, rice sheath blight disease, wheat scab, grape anthracnose and pine needle brown spot; the occurrence of various crop diseases can be effectively reduced; economic losses are reduced. Meanwhile, the prepared bactericide has the advantages of rapid effect-taking, long lasting period, and no residues due to natural properties; the extracting technology is simple, the equipment investment is low, low carbon and environmental-protection are achieved and the growth of plants can be promoted in the use process ; the bactericide is an ideal agricultural bactericide.
Description
Technical field
The invention belongs to natural bactericidal agent technical field, be specially a kind of bactericide containing strawberry tree Litter-fall extract and uses thereof.
Background technology
Agricultural chemicals is to controlling agricultural pest, guaranteeing that agricultural production and income plays an important role.In the course of bactericide development, synthetic chemistry bactericide is antibacterial pathogen, sterilization, and controlling crop diseases and insect pests guarantees that the aspects such as farm-forestry crop is stable also contribute huge.But shortcoming and long-term unreasonable use due to synthetic chemistry bactericide itself, expose gradually some serious problems: as damage by disease and insect develops immunity to drugs, residue of pesticide and the damage by disease and insect causing due to killed natural enemies are rampant again, bring hidden danger to people's health.In order to improve the mankind's living environment quality and food quality, countries in the world are all in the research that reduces chemical pesticide consumption, the developing direction of agricultural chemicals from high poison, high residue, grow the biopesticide that persistent synthetic chemistry agricultural chemicals turns to high security, low-residual, nuisanceless, high bioactivity, high selectivity.
Botanical fungicide refers to some active part of plant or extracts the medicament for controlling plant diseases that its active ingredient processes.Plant is the natural treasure-house of bioactive compound, the secondary metabolite of its generation is over 400,000 kinds, most of chemical substances wherein, as the amino acid of terpenes, alkaloid, flavonoids, steroidal, phenols, uniqueness and polysaccharide etc. all have desinsection or antibacterial activity.Therefore, plant is considered to chemosynthesis bactericide substitute exploiting natural resources the earliest.Since the eighties in 20th century, the screening operation of relevant restraining and sterilizing bacteria plant is at home and abroad carried out successively.Result of study demonstration, whole world restraining and sterilizing bacteria plant resources is extremely abundant, and major part concentrates in the plants such as composite family, pulse family, Liliaceae, Zingiber, Labiatae, Rutaceae, Myrtaceae, grass family and Lauraceae.In recent years, domestic and international many scholars investigated and had studied antibacterial, the bactericidal activity of some plant extractss.Ying Lao etc. are by the antibacterial biological activity determination of 47 Plants acetone extracts to several pathogens, find to contain agricultural antimicrobial component in cubeb litsen tree, Magnolia bilola and Michelia maudiae extract, and antimicrobial spectrum is wider, as botanical fungicide, be worth further research.Chen Jiao etc. control research by 58 Plant Extracts to the morbidity of the zoosporangium Germination suppression rate of Plasmopara viticola and inoculation leaf disc, filter out kiss-me, yellow wingceltis, masson pine, tree peony, 5 kinds of plant extraction liquids with bacteriostasis of locust tree.In addition, antibacterial, the bactericidal activity of the plant such as Umbelliferae, Celastraceae, Curcurbitaceae, Moraceae all had report.Red bayberry is Myruca ceas red bayberry spp.ing plants, is distributed widely in south China each province.Chinese waxmyrtle bark bitter, warm in nature, has the effect of the hemostasis of the loose stasis of blood, pain relieving, is among the peoplely used for the treatment of traumatic injury, fracture, dysentery, gastric duodenal ulcer, toothache etc.Bibliographical information Chinese waxmyrtle bark is mainly containing compounds such as flavonoids, triterpenes and diaryl heptane classes.Flavonoids, the main chemical compositions that myricetrin especially is wherein Chinese waxmyrtle bark, content reaches 10.19%.The chemical constitution study of Chinese waxmyrtle bark is found to Chinese waxmyrtle bark is worth develop and useining into-step as the resource plant of myricetrin.Bibliographical information, Chinese waxmyrtle bark extract has obvious step-down, the multiple effect such as hypoglycemic and antibacterial, and the present invention utilizes the sterilization component in its Litter-fall, has been developed to a kind of botanical fungicide.
Botanical fungicide, as an important component part of biorational agricultural chemicals, meets the requirement of people to modern, and exploitation environmental pollution is little, and is to meet social demand to person poultry safety's novel plant source bactericide.We have advantageous Natural resources condition at present, as long as strengthen the input of manpower and material resources, will obtain gratifying achievement.
Summary of the invention
For the above-mentioned problems in the prior art, the object of the invention is to design provides the technical scheme of a kind of bactericide containing strawberry tree Litter-fall extract and uses thereof, the natural noresidue of bactericide making, and can be dissolved in water, extraction process low-carbon environment-friendly; Can use by common method, use with susceptible and can play prevention effect before, for after susceptible, can play therapeutic action; Simultaneously in use can Promoting plant growth, use safety, reliable.
Described a kind of bactericide containing strawberry tree Litter-fall extract, is characterized in that containing strawberry tree Litter-fall extract 17-38%, auxiliary agent 2-15%, solvent 51-78%; Described auxiliary agent is more than one mixtures in ethoxylated dodecyl alcohol, JFC, tween, induced by alkyl hydroxybenzene polyethers, and described solvent is more than one mixtures in distilled water, methyl alcohol and ethanol;
Described strawberry tree Litter-fall extract adopts following methods preparation:
1) collecting the strawberry tree leaf, bark, the fruit that naturally wither and fall in field is raw material, be crushed to 200-450 order, add 2-3 doubly to the water of raw material weight, the water-bath constant temperature that is placed in 100 ℃ keeps 8-15h, then with ultrasonic, process 2-3 time, each 3-4min, the final vacuum suction filtration of finishing dealing with carries out Separation of Solid and Liquid, collect filtrate, standby;
2) by step 2) in filtrate be placed in sterilizing 15-20min at autoclave 120-130 ℃, be cooled to 55-60 ℃, then rotary distillation concentrates at 55-65 ℃, is concentrated into the 4-6% of original volume, collects concentrate, strawberry tree Litter-fall extract.
Described a kind of bactericide containing strawberry tree Litter-fall extract, is characterized in that the content of strawberry tree Litter-fall extract is 20-35%, is preferably 25-30%.
Described a kind of bactericide containing strawberry tree Litter-fall extract, is characterized in that the content of auxiliary agent is 3-13%, is preferably 5-11%.
Described a kind of bactericide containing strawberry tree Litter-fall extract, is characterized in that the content of solvent is 60-75%, is preferably 65-70%.
Described a kind of bactericide containing strawberry tree Litter-fall extract, is characterized in that in strawberry tree Litter-fall extract preparation method step 1): the water-bath time is 9-13h, preferably 10-12 h.
Described a kind of bactericide containing strawberry tree Litter-fall extract, it is characterized in that strawberry tree Litter-fall extract preparation method step 2) in: sterilization time is 16-18min, be cooled to 58 ℃, then rotary distillation concentrates at 60 ℃, is concentrated into 5% of original volume.
The application of described a kind of bactericide containing strawberry tree Litter-fall extract in the preparation of preparation control graw mold of tomato, rice sheath blight disease, wheat scab, anthracnose of grape, Brown Spot of Pine Needles.
Above-mentioned a kind of bactericide containing strawberry tree Litter-fall extract, take the strawberry tree Litter-fall of pure natural as raw material is prepared from, and has very strong antibacterial and bactericidal effect; Graw mold of tomato, rice sheath blight disease, wheat scab, anthracnose of grape, Brown Spot of Pine Needles are all had to very strong kill activity, can effectively reduce the generation of crop multiple diseases, reduce economic loss, meanwhile, the medicament that makes is rapid-action, the lasting period long, natural noresidue; Its extraction process is easy, and equipment investment is few, and low-carbon environment-friendly in use can Promoting plant growth, is a kind of desirable agriculture bactericidal agent.
The percentage composition relating in present specification unless otherwise indicated, other the weight percentage that is pure material.
Embodiment
Now, in conjunction with embodiments of the invention and the test of pesticide effectiveness, further illustrate beneficial effect of the present invention.
Embodiment 1
The strawberry tree leaf, bark, the fruit that are withered and fallen naturally in field are crushed to 300 orders, add 2 times to the water of raw material weight, and the water-bath constant temperature that is placed in 100 ℃ keeps 13h, with ultrasonic, process 2 times, each 3min, the final vacuum suction filtration of finishing dealing with carries out Separation of Solid and Liquid, collects filtrate, then filtrate is placed in to sterilizing 15min at 125 ℃ of autoclaves, be cooled to 60 ℃, then rotary distillation concentrates at 63 ℃, is concentrated into 5% of original volume, collect concentrate, obtain strawberry tree Litter-fall extract.In 100L reactor, drop into 60kg distilled water, then drop into 29kg strawberry tree Litter-fall extract, finally slowly drop into 5kg ethoxylated dodecyl alcohol and 6kgJFC, stir about one hour to being uniformly dissolved completely, final bactericide.
Embodiment 2
The strawberry tree leaf, bark, the fruit that are withered and fallen naturally in field are crushed to 200 orders, add 2 times to the water of raw material weight, and the water-bath constant temperature that is placed in 100 ℃ keeps 11h, with ultrasonic, process 3 times, each 3min, the final vacuum suction filtration of finishing dealing with carries out Separation of Solid and Liquid, collects filtrate, then filtrate is placed in to sterilizing 15min at 125 ℃ of autoclaves, be cooled to 60 ℃, then rotary distillation concentrates at 61 ℃, is concentrated into 5% of original volume, collect concentrate, obtain strawberry tree Litter-fall extract.In 100L reactor, drop into 73kg methyl alcohol, then drop into 22kg strawberry tree Litter-fall extract, finally slowly drop into 2kgJFC and 3kg induced by alkyl hydroxybenzene polyethers, stir about one hour to being uniformly dissolved completely, final bactericide.
Embodiment 3
The strawberry tree leaf, bark, the fruit that are withered and fallen naturally in field are crushed to 450 orders, add 2 times to the water of raw material weight, and the water-bath constant temperature that is placed in 100 ℃ keeps 10h, with ultrasonic, process 2 times, each 3min, the final vacuum suction filtration of finishing dealing with carries out Separation of Solid and Liquid, collects filtrate, then filtrate is placed in to sterilizing 15min at 125 ℃ of autoclaves, be cooled to 60 ℃, then rotary distillation concentrates at 59 ℃, is concentrated into 5% of original volume, collect concentrate, obtain strawberry tree Litter-fall extract.In 100L reactor, drop into 71kg ethanol, then drop into 23kg strawberry tree Litter-fall extract, finally slowly drop into 2kg ethoxylated dodecyl alcohol, 2kgJFC and 2kg tween, stir about one hour to being uniformly dissolved completely, final bactericide.
Embodiment 4
The strawberry tree leaf, bark, the fruit that are withered and fallen naturally in field are crushed to 250 orders, add 2 times to the water of raw material weight, and the water-bath constant temperature that is placed in 100 ℃ keeps 9h, with ultrasonic, process 2 times, each 3min, the final vacuum suction filtration of finishing dealing with carries out Separation of Solid and Liquid, collects filtrate, then filtrate is placed in to sterilizing 15min at 125 ℃ of autoclaves, be cooled to 60 ℃, then rotary distillation concentrates at 57 ℃, is concentrated into 5% of original volume, collect concentrate, obtain strawberry tree Litter-fall extract.In 100L reactor, drop into 39kg distilled water and 30kg ethanol, then drop into 24kg strawberry tree Litter-fall extract, finally slowly drop into 4kg tween and 3kg induced by alkyl hydroxybenzene polyethers, stir about one hour to being uniformly dissolved completely, final bactericide.
Embodiment 5
The strawberry tree leaf, bark, the fruit that are withered and fallen naturally in field are crushed to 350 orders, add 2 times to the water of raw material weight, and the water-bath constant temperature that is placed in 100 ℃ keeps 15h, with ultrasonic, process 3 times, each 3min, the final vacuum suction filtration of finishing dealing with carries out Separation of Solid and Liquid, collects filtrate, then filtrate is placed in to sterilizing 15min at 125 ℃ of autoclaves, be cooled to 60 ℃, then rotary distillation concentrates at 65 ℃, is concentrated into 5% of original volume, collect concentrate, obtain strawberry tree Litter-fall extract.In 100L reactor, drop into 20kg distilled water, 20kg methyl alcohol and 27kg ethanol, drop into again 25kg strawberry tree Litter-fall extract, finally slowly drop into 2kg ethoxylated dodecyl alcohol, 2kgJFC, 2kg tween and 2kg induced by alkyl hydroxybenzene polyethers, stir about one hour to being uniformly dissolved completely, final bactericide.
Embodiment 6
The strawberry tree leaf, bark, the fruit that are withered and fallen naturally in field are crushed to 400 orders, add 2 times to the water of raw material weight, and the water-bath constant temperature that is placed in 100 ℃ keeps 8h, with ultrasonic, process 3 times, each 3min, the final vacuum suction filtration of finishing dealing with carries out Separation of Solid and Liquid, collects filtrate, then filtrate is placed in to sterilizing 15min at 125 ℃ of autoclaves, be cooled to 60 ℃, then rotary distillation concentrates at 55 ℃, is concentrated into 5% of original volume, collect concentrate, obtain strawberry tree Litter-fall extract.In 100L reactor, drop into 43kg methyl alcohol and 20kg ethanol, drop into again 27kg strawberry tree Litter-fall extract, finally slowly drop into 5kg ethoxylated dodecyl alcohol and 5kg induced by alkyl hydroxybenzene polyethers, stir about one hour to being uniformly dissolved completely, final bactericide.
The control efficiency of above-mentioned bactericide is below described by test.
The test effect of table 1 embodiment 1-6 to graw mold of tomato
| Process | Regression equation | Correlation coefficient | Significance level | EC 50 |
| Embodiment 1 | Y=0.615X+2.159 | 0.911 | 0.016 | 35.1526 |
| Embodiment 2 | Y=0.537X+4.157 | 0.816 | 0.134 | 37.1375 |
| Embodiment 3 | Y=0.539X+4.112 | 0.923 | 0.031 | 32.2376 |
| Embodiment 4 | Y=0.572X+5.104 | 0.946 | 0.046 | 35.5647 |
| Embodiment 5 | Y=0.534X+2.368 | 0.937 | 0.107 | 37.4457 |
| Embodiment 6 | Y=0.498X+5.017 | 0.827 | 0.121 | 39.4888 |
The test effect of table 2 embodiment 1-6 to anthracnose of grape
| Process | Regression equation | Correlation coefficient | Significance level | EC 50 |
| Embodiment 1 | Y=0.2376X+2.3247 | 0.816 | 0.000 | 47.3516 |
| Embodiment 2 | Y=0.2013X+2.1028 | 0.945 | 0.009 | 45.2475 |
| Embodiment 3 | Y=0.1968X+2.8451 | 0.932 | 0.016 | 45.3516 |
| Embodiment 4 | Y=0.2514X+2.3156 | 0.975 | 0.003 | 48.3610 |
| Embodiment 5 | Y=1.1917X+3.2417 | 0.843 | 0.013 | 43.7435 |
| Embodiment 6 | Y=0.2530X+3.4421 | 0.952 | 0.031 | 47.1019 |
The test effect of table 3 embodiment 1-6 to Brown Spot of Pine Needles
| Process | Regression equation | Correlation coefficient | Significance level | EC 50 |
| Embodiment 1 | Y=0.3721X+3.2571 | 0.931 | 0.037 | 35.4571 |
| Embodiment 2 | Y=0.3891X+2.1257 | 0.900 | 0.026 | 39.1923 |
| Embodiment 3 | Y=0.4125X+3.7416 | 0.867 | 0.033 | 38.3109 |
| Embodiment 4 | Y=0.3917X+4.2526 | 0.816 | 0.092 | 35.4740 |
| Embodiment 5 | Y=0.4173X+4.3247 | 0.000 | 0.001 | 37.2574 |
| Embodiment 6 | Y=0.3916X+3.1528 | 0.856 | 0.115 | 33.3679 |
Table 1,2,3 result of the tests show: embodiment 1-6 all has higher control efficiency to graw mold of tomato, anthracnose of grape, Brown Spot of Pine Needles.In the process of actual production and use, can, by adjusting the extension rate of medicament, reach the object of Optimal Control disease.
Claims (7)
1. containing a bactericide for strawberry tree Litter-fall extract, it is characterized in that containing strawberry tree Litter-fall extract 17-38%, auxiliary agent 2-15%, solvent 51-78%; Described auxiliary agent is more than one mixtures in ethoxylated dodecyl alcohol, JFC, tween, induced by alkyl hydroxybenzene polyethers, and described solvent is more than one mixtures in distilled water, methyl alcohol and ethanol;
Described strawberry tree Litter-fall extract adopts following methods preparation:
1) collecting the strawberry tree leaf, bark, the fruit that naturally wither and fall in field is raw material, be crushed to 200-450 order, add 2-3 doubly to the water of raw material weight, the water-bath constant temperature that is placed in 100 ℃ keeps 8-15h, then with ultrasonic, process 2-3 time, each 3-4min, the final vacuum suction filtration of finishing dealing with carries out Separation of Solid and Liquid, collect filtrate, standby;
2) by step 2) in filtrate be placed in sterilizing 15-20min at autoclave 120-130 ℃, be cooled to 55-60 ℃, then rotary distillation concentrates at 55-65 ℃, is concentrated into the 4-6% of original volume, collects concentrate, strawberry tree Litter-fall extract.
2. a kind of bactericide containing strawberry tree Litter-fall extract as claimed in claim 1, is characterized in that the content of strawberry tree Litter-fall extract is 20-35%, is preferably 25-30%.
3. a kind of bactericide containing strawberry tree Litter-fall extract as claimed in claim 1, is characterized in that the content of auxiliary agent is 3-13%, is preferably 5-11%.
4. a kind of bactericide containing strawberry tree Litter-fall extract as claimed in claim 1, is characterized in that the content of solvent is 60-75%, is preferably 65-70%.
5. a kind of bactericide containing strawberry tree Litter-fall extract as claimed in claim 1, is characterized in that in strawberry tree Litter-fall extract preparation method step 1): the water-bath time is 9-13h, preferably 10-12 h.
6. a kind of bactericide containing strawberry tree Litter-fall extract as claimed in claim 1, it is characterized in that strawberry tree Litter-fall extract preparation method step 2) in: sterilization time is 16-18min, be cooled to 58 ℃, then rotary distillation concentrates at 60 ℃, is concentrated into 5% of original volume.
7. the application of a kind of bactericide containing strawberry tree Litter-fall extract as claimed in claim 1 in the preparation of preparation control graw mold of tomato, rice sheath blight disease, wheat scab, anthracnose of grape, Brown Spot of Pine Needles.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310725507.9A CN103689001B (en) | 2013-12-25 | 2013-12-25 | A kind of sterilant containing Hairy Waxmyrtle litter extracting solution and purposes thereof |
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| CN201310725507.9A CN103689001B (en) | 2013-12-25 | 2013-12-25 | A kind of sterilant containing Hairy Waxmyrtle litter extracting solution and purposes thereof |
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| CN103689001A true CN103689001A (en) | 2014-04-02 |
| CN103689001B CN103689001B (en) | 2016-06-01 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104782693A (en) * | 2015-04-06 | 2015-07-22 | 孟凯涛 | Preparation method of composition for preventing and controlling brown mildew of Yangshan juicy peaches |
| CN105309526A (en) * | 2015-11-18 | 2016-02-10 | 无锡市稼宝药业有限公司 | Botanical fungicide for anthracnose disease |
| CN105384733A (en) * | 2015-11-02 | 2016-03-09 | 许自协 | Compound inhibiting lecanosticta acicola growth |
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| JPH06183926A (en) * | 1991-10-09 | 1994-07-05 | Senjo Gakuen | Production of antibacterial cloth using herb drug dye and tannin mordant |
| JP3000217B2 (en) * | 1997-11-24 | 2000-01-17 | モレックス インコーポレーテッド | Terminal blade attached to flexible substrate |
| CN101099496A (en) * | 2007-07-27 | 2008-01-09 | 浙江林学院 | Gale branch and leaves extraction disinfectant and its producing method |
| CN103340466A (en) * | 2013-06-17 | 2013-10-09 | 浙江大学 | Use of freshly squeezed red bayberry juice in killing of escherichia coli |
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2013
- 2013-12-25 CN CN201310725507.9A patent/CN103689001B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06183926A (en) * | 1991-10-09 | 1994-07-05 | Senjo Gakuen | Production of antibacterial cloth using herb drug dye and tannin mordant |
| JP3000217B2 (en) * | 1997-11-24 | 2000-01-17 | モレックス インコーポレーテッド | Terminal blade attached to flexible substrate |
| CN101099496A (en) * | 2007-07-27 | 2008-01-09 | 浙江林学院 | Gale branch and leaves extraction disinfectant and its producing method |
| CN103340466A (en) * | 2013-06-17 | 2013-10-09 | 浙江大学 | Use of freshly squeezed red bayberry juice in killing of escherichia coli |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104782693A (en) * | 2015-04-06 | 2015-07-22 | 孟凯涛 | Preparation method of composition for preventing and controlling brown mildew of Yangshan juicy peaches |
| CN105384733A (en) * | 2015-11-02 | 2016-03-09 | 许自协 | Compound inhibiting lecanosticta acicola growth |
| CN105309526A (en) * | 2015-11-18 | 2016-02-10 | 无锡市稼宝药业有限公司 | Botanical fungicide for anthracnose disease |
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| CN103689001B (en) | 2016-06-01 |
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