CN104782693A - Preparation method of composition for preventing and controlling brown mildew of Yangshan juicy peaches - Google Patents
Preparation method of composition for preventing and controlling brown mildew of Yangshan juicy peaches Download PDFInfo
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- CN104782693A CN104782693A CN201510160912.XA CN201510160912A CN104782693A CN 104782693 A CN104782693 A CN 104782693A CN 201510160912 A CN201510160912 A CN 201510160912A CN 104782693 A CN104782693 A CN 104782693A
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Abstract
The invention relates to a preparation method of composition for preventing and controlling brown mildew of Yangshan juicy peaches. The method comprises the steps as follows: weighing 150 g of tirpitzia ovoidea, 130 g of Chinese enkianthus, 135 g of microsorum insigne, 120 g of fernleaf bambusa, 100 g of Chinese starjasmine stems, 80 g of chervil larkspur herb, 90 g of twigs and leaves of taperleaf Japanese spiraea herb and 60 g of pogonatherum crinitum; smashing the raw materials, sieving the materials with a 60-mesh sieve, adding 25 L of water, heating the mixture at the temperature of 100 DEG C for 5 hours in a flask equipped with a condenser, filtering the mixture, cooling filtrate to the room temperature and loading a product in a sprayer to obtain the composition. Preventing and controlling of the brown mildew of Yangshan juicy peaches refers to killing of aspergillus niger ATCC 16404 of the Yangshan juicy peaches.
Description
Technical field
The present invention relates to agriculture field, relate in particular to a kind of preparation method preventing and treating the composition of Yangshan honey peach brown mould bacterium.
Background technology
Current use chemical bactericide is the Main Means of control Yangshan honey peach germ, but the frequent chemical bactericide that uses makes phytopathogen create pesticide resistance, not only effectively can not control disease, also add environmental pollution.Although the component of the bactericide based on natural traditional Chinese medicine shared by present in All Around The World plant protection market is very low, is subject to the extensive attention of every profession and trade, has become focus and the emphasis of our times agricultural research.
Summary of the invention
The object of this invention is to provide a kind of preparation method preventing and treating the composition of Yangshan honey peach brown mould bacterium.
In order to realize object of the present invention, the invention provides a kind of preparation method preventing and treating the composition of Yangshan honey peach brown mould bacterium, the method comprises the following steps: that taking rice reads fragrant plant 150 grams, Chinese enkianthus 130 grams, plumage splits star fern 135 grams, fernleaf hedge bamboo 120 grams, caulis trachelospermi 100 grams, chervil lankspur herb 80 grams, blowpipe 90 grams and rough melic herb 60 grams, pulverize, cross 60 mesh sieves, add 25 liters, water, heat 5 hours at 100 DEG C in the flask that condenser is housed, filter, filtrate is cooled to room temperature, load in sprayer and get final product, honey peach brown mould bacterium refers to the black-koji mould ATCC16404 killing Yangshan honey peach in described control Yangshan.
In the present compositions, fragrant plant read by rice is monarch drug in a prescription; Chinese enkianthus, plumage split star fern, fernleaf hedge bamboo is ministerial drug; Caulis trachelospermi, chervil lankspur herb, blowpipe are adjutant; Rough melic herb is for making medicine.
The successful of the composition of control Yangshan of the present invention honey peach brown mould bacterium, and be all made up of natural drug, environment is not polluted, is therefore worthy of popularization.
Embodiment
The present invention is further illustrated below by embodiment.It should be understood that embodiments of the invention are for illustration of the present invention instead of limitation of the present invention.Essence according to the present invention all belongs to the scope of protection of present invention to the simple modifications that the present invention carries out.Unless otherwise stated, the percentage in the present invention is percetage by weight.
Embodiment 1 spray of the present invention
Take that fragrant plant 150 grams read by rice, Chinese enkianthus 130 grams, plumage split star fern 135 grams, fernleaf hedge bamboo 120 grams, caulis trachelospermi 100 grams, chervil lankspur herb 80 grams, blowpipe 90 grams and rough melic herb 60 grams, pulverize, cross 60 mesh sieves, add 25 liters, water, heat 5 hours at 100 DEG C in the flask that condenser is housed, filter, filtrate is cooled to room temperature, loads in sprayer and namely obtains spray of the present invention.
The preparation of embodiment 2 spray 2 of the present invention
Prepare spray 2 according to the mode identical with embodiment 1, difference is that not adding rice reads fragrant plant.
The preparation of embodiment 3 spray 3 of the present invention
Prepare spray 3 according to the mode identical with embodiment 1, difference is not add Chinese enkianthus.
The preparation of embodiment 4 spray 4 of the present invention
Prepare spray 4 according to the mode identical with embodiment 1, difference is that not adding plumage splits star fern.
The preparation of embodiment 5 spray 5 of the present invention
Prepare spray 5 according to the mode identical with embodiment 1, difference is not add fernleaf hedge bamboo.
The preparation of embodiment 6 spray 6 of the present invention
Prepare spray 6 according to the mode identical with embodiment 1, difference is not add caulis trachelospermi.
The preparation of embodiment 7 spray 7 of the present invention
Prepare spray 7 according to the mode identical with embodiment 1, difference is not add chervil lankspur herb.
The preparation of embodiment 8 spray 8 of the present invention
Prepare spray 8 according to the mode identical with embodiment 1, difference is not add blowpipe.
The preparation of embodiment 9 spray 9 of the present invention
Prepare spray 9 according to the mode identical with embodiment 1, difference is not add rough melic herb.
Experimental example bacteriostatic test
Filter paper enzyme classical in this area is adopted to carry out bacteriostatic test, wherein:
1. material
1.1 for examination bacterial classification
Black-koji mould ATCC16404 is purchased from Fu Xiang bio tech ltd, Shanghai.
1.2 culture fluid
Nutrient agar and nutrient broth, purchased from Chen Yu experimental facilities Co., Ltd of BeiJing ZhongKe.
1.3 test method
Black-koji mould ATCC16404 is inoculated in agar plate nutrient solution plane, evenly gathers during inoculation.The aseptic circular filter paper sheet of tweezer, sprays the spray prepared in embodiment 1-9 respectively, to complete wetting, is attached in the agar plate nutrient solution plane of inoculated bacteria.Agar plate is placed in the incubator of 37 DEG C, incubation 4 hours.Measure the diameter of bacterial restrain.Measure and average for 3 times.
1.4 result
The Disinfection Effect of each spray sees the following form.
| The inhibition zone diameter (mm) of black-koji mould ATCC16404 | |
| Embodiment 1 | 17.13 |
| Embodiment 2 | 11.58 |
| Embodiment 3 | 10.81 |
| Embodiment 4 | 10.75 |
| Embodiment 5 | 8.62 |
| Embodiment 6 | 8.77 |
| Embodiment 7 | 9.15 |
| Embodiment 8 | 8.35 |
| Embodiment 9 | 7.96 |
Inhibition zone diameter is larger, illustrates that fungistatic effect is better.From the result of upper table, the spray of embodiment 1 has the strongest bactericidal effect, and its sterilizing ability is better than the spray prepared in other embodiments far away.
Claims (1)
1. prevent and treat the preparation method of the composition of Yangshan honey peach brown mould bacterium for one kind, the method can comprise the following steps: to take that fragrant plant 150 grams read by rice, Chinese enkianthus 130 grams, plumage split star fern 135 grams, fernleaf hedge bamboo 120 grams, caulis trachelospermi 100 grams, chervil lankspur herb 80 grams, blowpipe 90 grams and rough melic herb 60 grams, pulverize, cross 60 mesh sieves, add 25 liters, water, heat 5 hours at 100 DEG C in the flask that condenser is housed, filter, filtrate is cooled to room temperature, load in sprayer and get final product, described control Yangshan honey peach brown mould bacterium refers to the black-koji mould ATCC16404 killing Yangshan honey peach.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510160912.XA CN104782693A (en) | 2015-04-06 | 2015-04-06 | Preparation method of composition for preventing and controlling brown mildew of Yangshan juicy peaches |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510160912.XA CN104782693A (en) | 2015-04-06 | 2015-04-06 | Preparation method of composition for preventing and controlling brown mildew of Yangshan juicy peaches |
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| CN104782693A true CN104782693A (en) | 2015-07-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510160912.XA Pending CN104782693A (en) | 2015-04-06 | 2015-04-06 | Preparation method of composition for preventing and controlling brown mildew of Yangshan juicy peaches |
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Patent Citations (7)
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| CN101062926A (en) * | 2005-12-14 | 2007-10-31 | 南开大学 | Preparation and usage of unnatural 3,4-dihydro isocoumarin derivatives |
| CN1943361A (en) * | 2006-11-02 | 2007-04-11 | 四川大学 | Spiraeic germicide and its preparing method and use |
| CN102784350A (en) * | 2012-08-07 | 2012-11-21 | 孙雅儒 | Mite killing bactericide and preparation method of mite killing bactericide |
| CN103232942A (en) * | 2013-04-22 | 2013-08-07 | 安徽农业大学 | Preparation method of anti-phytopathogen fermentation liquid of spirea endophytic fungus |
| CN103689001A (en) * | 2013-12-25 | 2014-04-02 | 浙江农林大学天目学院 | Bactericide containing arbutus litter extract and purpose of bactericide |
| CN104054759A (en) * | 2014-06-22 | 2014-09-24 | 张永福 | Insectofungicide for crops prepared from Chinese medicinal herbs |
| CN104381348A (en) * | 2014-11-28 | 2015-03-04 | 李晓枫 | Botanical fungicide for preventing and controlling peach tree bleeding disease and preparation method of botanical fungicide for preventing and controlling peach tree bleeding disease |
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| 中国农业科学院植物保护研究所,: "《中国植物保护科学》", 30 September 1961, 科学出版社 * |
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Application publication date: 20150722 |
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