CN113603545B - Plant nutrition conditioning liquid containing pine needle extract and application thereof - Google Patents
Plant nutrition conditioning liquid containing pine needle extract and application thereof Download PDFInfo
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- CN113603545B CN113603545B CN202111084040.5A CN202111084040A CN113603545B CN 113603545 B CN113603545 B CN 113603545B CN 202111084040 A CN202111084040 A CN 202111084040A CN 113603545 B CN113603545 B CN 113603545B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
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- Organic Chemistry (AREA)
- Plant Pathology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a plant nutrient conditioning solution, which belongs to the technical field of crops, wherein a pine needle extract is used as an effective active ingredient of the plant nutrient conditioning solution, so that the plant nutrient conditioning solution has the effect of remarkably promoting the growth of plants, and the yield and the quality of the crops are remarkably improved.
Description
Technical Field
The invention relates to the technical field of crops, in particular to a plant nutrition conditioning liquid containing a pine needle extract and application thereof.
Background
Crop nutrition is the material basis of agricultural production, but at present, excessive application of chemical fertilizers causes serious problems of soil structure deterioration, fertility reduction and the like, the crop health, the crop quality and the yield are seriously influenced, and meanwhile, the food safety and the environment are adversely affected. N, P, K elements in the fertilizer are necessary nutrient elements for plant growth and development, and are also the material basis of the fertilizer efficiency of the conventional fertilizer, and by utilizing the elements and the photosynthesis products thereof, plants can synthesize primary metabolites such as amino acid, protein, nucleotide, polysaccharide and the like necessary for the growth and development of the plants through primary metabolism, but the substances can only ensure the growth and development of the plants and cannot ensure the survival and development of the plants under natural conditions, so that the development of novel crop nutrition products which are environment-friendly, remarkable in fertilizer efficiency and low in cost is an important requirement for the development of modern green agriculture.
In addition, the pesticide used in the agricultural production is mainly chemical pesticide, although harmful pathogenic bacteria on crops can be killed, a large part of agricultural products are lost due to too high pesticide residue components, and the chemical pesticide can cause pesticide poisoning accidents of people and livestock. Therefore, farmers are eagerly looking for a pesticide which has good effect of preventing and controlling plant germs, is harmless to people and livestock, and has low price, so as to improve the safety and environmental protection of agricultural products and reduce the production cost.
The plant source pesticide has the characteristics of quick biodegradation in the environment, low toxicity to people, livestock and non-target organisms, difficulty in generating resistance to pests, low cost, easiness in obtaining and the like, meets the era requirements of environmental protection, health and continuous development of modern agriculture, has extremely high development value and is more and more valued. Although some results have been achieved in the development of biological pesticides, the development of plant-derived pesticides is still in the initial stage, and the wide search for ideal plant-derived pesticides is still slow.
The pine needle is leaves of plants in Pinus of Pinaceae, also called pine hair, and is mainly derived from Pinus massoniana, Pinus armandii, Pinus pumila, Pinus thunbergii, Pinus tabulaeformis, Pinus koraiensis, and Pinus koraiensis. Pine needles are one of the main byproducts of pine plants, and are a natural renewable resource which has the advantages of high regeneration speed, wide distribution, large natural accumulation and sustainable utilization, and can be harvested all the year round. Pine needles are widely used in medicines and foods because of containing a large amount of active volatile substances and rich nutritional ingredients, such as amino acids, terpenes, volatile oil, abscisic acid, a large amount of water-soluble vitamin B, sugar, carotene, chlorophyll, flavonoids, more than ten trace elements, crude protein and the like, but the research on simultaneously promoting plant growth of plant pathogenic bacteria is very little. Therefore, the pine needle extract is used for deeply researching plant pathogenic bacteria and promoting plant growth, and researching and developing a novel plant nutrition conditioning solution, thereby providing an important theoretical basis for comprehensively developing and utilizing pine needles.
Disclosure of Invention
The invention aims to provide a plant nutrient conditioning solution which can effectively improve the yield of crops and can effectively inhibit plant pathogenic bacteria.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a plant nutrition conditioning liquid, which takes a pine needle extract as an effective active ingredient, and the preparation method of the pine needle extract comprises the following steps:
(1) collecting fresh pine needles, removing impurities, cleaning, drying and crushing to obtain pine needle powder;
(2) mixing the pine needle powder with water, performing ultrasonic extraction, filtering, and concentrating supernatant to obtain a pine needle water extract and pine needle filter residue;
(3) mixing the pine needle filter residue with an ethanol solution, extracting, filtering, and concentrating supernatant to obtain a pine needle alcohol extract;
(4) and mixing the pine needle water extract and the pine needle alcohol extract to obtain the pine needle extract.
Preferably, the crushed particle size is 10-30 meshes.
Preferably, the mass volume ratio of the pine needle powder to the water is 1: 5-15, and the mass volume ratio of the pine needle filter residue to the ethanol solution is 1: 20-30.
Preferably, the volume percentage of the ethanol solution is 20-70%.
Preferably, in the step (2), the ultrasonic power is 150-250W, the temperature is 40-60 ℃, and the time is 0.5-1.5 h.
Preferably, in the step (2), the number of times of extraction is 1-3.
Preferably, in the step (3), the extraction temperature is 60-80 ℃, the extraction times are 2-3 times, and the extraction time is 3-5 h/time.
Preferably, the plant nutrition conditioning liquid is prepared by mixing the pine needle water extract and the pine needle alcohol extract according to the volume ratio of 1: 2-4.
The invention also provides application of the plant nutrient conditioning solution in promoting plant growth.
The invention also provides application of the plant nutrient conditioning solution in resisting plant pathogenic bacteria.
Compared with the prior art, the technical scheme of the invention has the following beneficial effects:
the plant nutrient conditioning solution contains various nutrient components required by plants, has the effect of remarkably promoting the growth of the plants, improves the yield of the crops, and can effectively prevent and control bitter gourd fusarium wilt pathogens, apple ring rot pathogens, tomato botrytis cinerea and other plant pathogens. The plant nutrition conditioning liquid has the advantages of easily available raw materials, rich sources and low cost, and is natural, pollution-free, green, environment-friendly and high in safety by taking the pine needle extract as an effective active ingredient.
Detailed Description
The invention provides a plant nutrition conditioning liquid, which takes a pine needle extract as an effective active ingredient, and the preparation method of the pine needle extract comprises the following steps:
(1) collecting fresh pine needles, removing impurities, cleaning, drying and crushing to obtain pine needle powder;
(2) mixing the pine needle powder with water, performing ultrasonic extraction, filtering, and concentrating supernatant to obtain a pine needle water extract and pine needle filter residue;
(3) mixing the pine needle filter residue with an ethanol solution, extracting, filtering, and concentrating supernatant to obtain the pine needle alcohol extract.
(4) And mixing the pine needle water extract and the pine needle alcohol extract to obtain the pine needle extract.
In the invention, the plant nutrient conditioning solution taking the pine needle extract as the effective active ingredient provides a plurality of nutrient components required by plant growth for plants, thereby promoting the plant growth, improving the stress resistance of the plants and improving the yield and the quality of crops.
In the invention, fresh pine needles are collected, cleaned to remove impurities, dried and crushed to obtain pine needle powder. The pulverized particle size is preferably 10 to 30 meshes, more preferably 15 to 25 meshes, and even more preferably 20 meshes. The invention promotes the dissolution of effective components in the subsequent extraction process by crushing.
In the invention, the pine needle powder is mixed with water, extracted by ultrasonic, filtered and concentrated to obtain pine needle water extract and pine needle filter residue. The mass-volume ratio of the pine needle powder to water is preferably 1: 5-15, and more preferably 1: 10. In the invention, the ultrasonic power is preferably 150-250W, and more preferably 200W; the ultrasonic temperature is preferably 40-60 ℃, and more preferably 50 ℃; the ultrasonic time is preferably 0.5-1.5 h, and more preferably 1 h; the number of times of extraction is preferably 1 to 3 times, and more preferably 2 times. The concentrated supernatant of the present invention may optionally be concentrated under reduced pressure to one-half of the volume.
In the invention, the pine needle filter residue is mixed with an ethanol solution, extracted, filtered and supernatant fluid is concentrated to obtain the pine needle alcohol extract. The mass volume ratio of the pine needle filter residue to the ethanol solution is preferably 1: 20-30, more preferably 1: 25. the volume percentage of the ethanol solution is preferably 20-70%, more preferably 40-60%, and even more preferably 50%. The extraction temperature is preferably 60-80 ℃, more preferably 65-75 ℃, and more preferably 70 ℃; the extraction times are preferably 2-3 times; the extraction time is preferably 3-5 h/time, and more preferably 4 h/time.
In the present invention, the pine needles preferably include pinus massoniana needle, black pine needle or Chinese pine needle.
In the invention, the plant nutrition conditioning liquid is preferably prepared by mixing a pine needle water extract and a pine needle alcohol extract according to a volume ratio of 1: 2-4. The formulation of the plant nutrient conditioning solution used in agriculture is not specially limited, and can be selected from aqueous solution, wettable powder, granules, missible oil or suspending agent. The plant nutrient conditioning liquid can be applied to the soil by spreading, root application and irrigating with water and fertilizer, and can also be prepared into an aqueous agent to be sprayed on the leaf surfaces. The plant nutrient conditioning solution contains a large amount of substances required by plant growth, promotes the plant growth, improves the yield and the quality of crops, can inhibit pathogenic bacteria in the crops and improves the stress resistance of the plants, and is green, environment-friendly, pollution-free and high in safety.
The invention also provides application of the plant nutrient conditioning solution in promoting plant growth.
The invention also provides application of the plant nutrient conditioning solution in resisting plant pathogenic bacteria.
In the present invention, all the raw material components are commercially available products well known to those skilled in the art unless otherwise specified.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A preparation method of a pine needle extract comprises the following specific steps:
(1) collecting fresh pinus massoniana needles, removing impurities, cleaning, drying, and crushing to 20 meshes to obtain pine needle powder;
(2) adding 10 times volume of water into pine needle powder, performing ultrasonic treatment at a power of 200W and a temperature of 50 ℃ for 1h, filtering, collecting filtrate, adding 10 times volume of water into filter residue, performing ultrasonic treatment at a power of 200W and a temperature of 50 ℃ for 1h, filtering, collecting filtrate, performing third extraction according to the method, combining the filtrates, and concentrating the supernatant under reduced pressure to one half of the volume to obtain pine needle water extract and pine needle filter residue;
(3) adding a 50% ethanol solution with the volume 25 times that of the pine needle filter residue, extracting at 70 ℃ for 4 hours, filtering, collecting filtrate, performing third extraction according to the method, combining the filtrates, and concentrating the supernatant under reduced pressure to one half of the volume to obtain the pine needle alcohol extract;
(4) and mixing the pine needle water extract and the pine needle alcohol extract to obtain the pine needle extract.
The plant nutrition conditioning liquid is obtained by uniformly mixing a pine needle water extract and a pine needle alcohol extract according to the volume ratio of 1: 3.
Example 2
A preparation method of a pine needle extract comprises the following specific steps:
(1) collecting fresh pinus massoniana needles, removing impurities, cleaning, drying, and crushing to 10 meshes to obtain pine needle powder;
(2) adding 5 times of water into pine needle powder, performing ultrasonic treatment at 40 ℃ for 0.5h at a power of 150W, filtering, collecting filtrate, adding 5 times of water into filter residue, performing ultrasonic treatment at 40 ℃ for 0.5h at a power of 150W, filtering, collecting filtrate, performing third extraction according to the method, combining the filtrates, and concentrating the supernatant under reduced pressure to one half of the volume to obtain pine needle water extract and pine needle filter residue;
(3) adding 30 times of 20% ethanol solution into the pine needle filter residue, extracting at 80 ℃ for 3h, filtering, collecting filtrate, adding 30 times of 20% ethanol solution into the filter residue, extracting at 80 ℃ for 3h, filtering, collecting filtrate, performing third extraction according to the method, combining the filtrates, and concentrating the supernatant under reduced pressure to one half of the volume to obtain the pine needle alcohol extract;
(4) and mixing the pine needle water extract and the pine needle alcohol extract to obtain the pine needle extract.
The plant nutrition conditioning liquid is obtained by uniformly mixing a pine needle water extract and a pine needle alcohol extract according to the volume ratio of 1: 4.
Example 3
A preparation method of a pine needle extract comprises the following specific steps:
(1) collecting fresh pinus massoniana needle, removing impurities, cleaning, drying, and then crushing to 30 meshes to obtain pine needle powder;
(2) adding 15 times of water into pine needle powder, performing ultrasonic treatment at the power of 250W and the temperature of 60 ℃ for 1.5h, filtering, collecting filtrate, adding 15 times of water into filter residue, performing ultrasonic treatment at the power of 250W and the temperature of 60 ℃ for 1.5h, filtering, collecting filtrate, performing third extraction according to the method, combining the filtrates, and concentrating the supernatant under reduced pressure to half of the volume to obtain pine needle water extract and pine needle filter residue;
(3) adding 20 times volume of 70% ethanol solution into the above folium Pini residue, extracting at 60 deg.C for 5h, filtering, collecting filtrate, adding 20 times volume of 70% ethanol solution into the residue, extracting at 60 deg.C for 5h, filtering, collecting filtrate, performing the third extraction according to the above method, mixing filtrates, and concentrating the supernatant under reduced pressure to half of the volume to obtain folium Pini ethanol extract;
(4) and mixing the pine needle water extract and the pine needle alcohol extract to obtain the pine needle extract.
The plant nutrition conditioning liquid is obtained by uniformly mixing a pine needle water extract and a pine needle alcohol extract according to the volume ratio of 1: 2.
Example 4
Determination of anti-plant pathogenic bacteria, the plant nutrition conditioning solution prepared in the example 1 is subjected to the determination of anti-plant pathogenic bacteria, and the specific test steps are as follows:
preparation of a culture medium: weighing 200g of peeled and cleaned potatoes, cutting into small pieces, adding 1L of distilled water, boiling until the potatoes are boiled thoroughly, filtering the potato pieces with gauze, adding 20g of glucose and 20g of agar powder, heating until the agar powder is completely melted, filtering with the gauze while hot, fixing the volume to 1L, subpackaging in a triangular flask, performing damp-heat sterilization at 121 ℃ for 30min, cooling, and storing at 4 ℃ for later use.
Preparing test liquid medicine: the plant nutrient conditioning solution prepared in example 1 was mixed with water to prepare a 10mg/mL solution.
Preparation of drug-containing medium (test group): the bacteriostatic activity of the plant nutrient conditioning solution prepared in example 1 on pathogenic bacteria to be tested is measured by a growth rate method, 0.5mL of test solution is added into a triangular flask, then melted culture medium is added, the mixture is fully shaken up and poured into a culture dish in equal volume, and the plant nutrient conditioning solution is obtained after cooling.
And (3) inoculating pathogenic bacteria: preparing a plurality of bacterial cakes on a cultured test plant pathogenic bacteria flat plate by using a puncher with the diameter of 0.6cm, picking and lightly placing the bacterial cakes on a plane of a medicament-containing culture medium by using an inoculating needle, placing 3 bacterial cakes in each culture medium, culturing for 24h at the temperature of 30 ℃, accurately measuring the diameter of bacterial colonies by using a ruler by adopting a cross method, taking the average value as the diameter of the bacterial colonies, calculating the inhibition rate, and using clear water instead of liquid medicine as a control group, wherein the result is shown in a table 1.
Inhibition (%) - ((control colony diameter-treated colony diameter)/control colony diameter. times.100%)
TABLE 1 inhibiting effect of plant nutrient conditioning liquid on different pathogenic bacteria
As can be seen from the table 1, the plant nutrient conditioning solution provided by the invention has good bacteriostatic action on bitter gourd fusarium wilt bacteria, apple ring rot bacteria and tomato gray mold bacteria, wherein the plant nutrient conditioning solution provided by the invention has the best bacteriostatic effect on bitter gourd fusarium wilt bacteria.
Example 5
After one week of transplanting the pod pepper seedlings, 2 liters of the plant nutrient conditioning solution of the embodiment 1 per mu is added according to the proportion of 1: 120 watering the roots; and then, in 15-20 days, using 2 liters of plant nutrient conditioning liquid per mu according to the weight ratio of 1: 80 watering the roots; then, in the flowering period, the plant nutrition conditioning solution is used according to the proportion of 1: 60, spraying the mixed water on the leaf surfaces to ensure that the leaf surfaces are fully soaked; during the fruiting period, the plant nutrient conditioning solution is used according to the weight ratio of 1: and 40, watering the roots, and applying for 2 times, wherein each time is 3 liters per mu. The same amount of clear water is used as a control group to replace the plant nutrition conditioning solution, the single fruit weight, the number of fruits per plant and the single plant fruit weight of the pepper in the harvest period are counted by 10 plants, the average value is taken, and the result is shown in table 2.
TABLE 2 EXAMPLE 1 Effect of plant Nutrition Conditioning solutions on the weight of individual fruits, number of fruits and weight of individual plants of Momordica charantia
| Treatment group | Weight per gram of single fruit | Number of fruiting per plant/ | Weight of single plant fruit/g |
| Plant nutrition conditioning liquid | 5.45 | 178.6 | 973.37 |
| Clear water control group | 4.38 | 82.5 | 361.35 |
As can be seen from table 2, the application of the plant nutrient conditioning solution of example 1 significantly improved the weight of each individual pepper, the number of cayenne pepper per plant, and the weight average of each individual pepper.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
1. The application of the plant nutrition conditioning liquid in resisting phytopathogen is characterized in that the plant nutrition conditioning liquid takes a pine needle extract as an effective active ingredient, and the preparation method of the pine needle extract comprises the following steps:
(1) collecting fresh pine needles, removing impurities, cleaning, drying and crushing to obtain pine needle powder;
(2) mixing the pine needle powder with water, performing ultrasonic extraction, filtering, and concentrating supernatant to obtain a pine needle water extract and pine needle filter residue; the ultrasonic power is 150-250W, the temperature is 40-60 ℃, and the time is 0.5-1.5 h;
(3) mixing the pine needle filter residue with an ethanol solution, extracting, filtering, and concentrating supernatant to obtain a pine needle alcohol extract;
(4) mixing the pine needle water extract and the pine needle alcohol extract to obtain a pine needle extract;
the plant pathogenic bacteria are ring rot of apple or botrytis cinerea.
2. The use according to claim 1, wherein the milled particle size is 10 to 30 mesh.
3. The application of claim 1, wherein the mass-to-volume ratio of the pine needle powder to water is 1: 5-15, and the mass-to-volume ratio of the pine needle filter residue to the ethanol solution is 1: 20-30.
4. The use according to claim 1, wherein the ethanol solution has a volume percentage of 20 to 70%.
5. The use of claim 1, wherein in the step (2), the number of times of extraction is 1-3 times.
6. The use according to claim 1, wherein in the step (3), the extraction temperature is 60-80 ℃, the extraction times are 2-3 times, and the extraction time is 3-5 h/time.
7. The application of any one of claims 1 to 6, wherein the plant nutrition conditioning liquid is obtained by mixing a pine needle water extract and a pine needle alcohol extract according to a volume ratio of 1: 2-4.
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| CN202111084040.5A CN113603545B (en) | 2021-09-14 | 2021-09-14 | Plant nutrition conditioning liquid containing pine needle extract and application thereof |
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| CN113603545B true CN113603545B (en) | 2022-08-30 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103141522B (en) * | 2013-03-15 | 2015-04-22 | 浙江师范大学 | Pine needle extract for preventing phytopathogen |
| CN113045357A (en) * | 2021-03-26 | 2021-06-29 | 中国科学院华南植物园 | Proanthocyanidin plant nutrient and application thereof |
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