CN103651607A - Biopesticide for vegetables and preparation method of biopesticide - Google Patents
Biopesticide for vegetables and preparation method of biopesticide Download PDFInfo
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- CN103651607A CN103651607A CN201310697192.1A CN201310697192A CN103651607A CN 103651607 A CN103651607 A CN 103651607A CN 201310697192 A CN201310697192 A CN 201310697192A CN 103651607 A CN103651607 A CN 103651607A
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Abstract
The invention discloses a biopesticide for vegetables and a preparation method of the biopesticide. The biopesticide is mainly prepared from the components in parts by weight as follows: 1 part of toyocamycin, 50-70 parts of common threewingnut roots, 60-80 parts of graceful jessamine herb, 40-60 parts of aconite, 50-70 parts of coptis chinensis, 40-60 parts of purslane, 30-50 parts of herba houttuyniae, 40-60 parts of common andrographis herb and 2-4 parts of a suspending agent. Compared with common chemical pesticides in the prior art, the obtained biopesticide for the vegetables has the characteristics of good effect and low toxicity and can be used as an ideal environment-friendly agricultural drug for vegetable protection; meanwhile, the toyocamycin is compounded with traditional Chinese medicine extracts, so that the sterilization and insecticidal spectrum can be enlarged, the efficacy can also be improved, and what's more, the problem of drug resistance caused by long-term usage of single pesticides can be solved.
Description
Technical field
The present invention relates to a kind of biopesticide, be specifically related to a kind of mainly by toyokamycin and the made compound bio agricultural chemicals of plurality of Chinese extract, be applied to agriculture field.
Background technology
Biological living and metabolite and the transgene product of the pests such as biopesticide refers to for anti-curing the disease, worm, grass, and can be made into the biogenic preparation of commodity, comprise the genetically modified plants of bacterium, virus, fungi, nematode, plant, Natural Enemies of Insects, farm antibiotics, plant growth regulator and Resistant crop smothering etc.Biopesticide is applied to the existing semicentennial history of agricultural production, but development is always comparatively slow for various reasons.In recent years, people more and more note the sustainable development of agricultural production and the coordination of human and environment, particularly difficult due to the toxic and side effect of chemical pesticide and screening novel pesticide, makes enterprise and researcher start notice to turn to research and development and the use of biopesticide.
Normally used chemical pesticide toxicity is large, in crops, have certain residual, thereby the mankind are had to potential threat.And often use the insecticide of single formula, not satisfactory for the effect of desinsection.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the invention provides a kind of vegetables biopesticide.
Technical scheme: for achieving the above object, a kind of vegetables biopesticide provided by the invention, it is mainly made by the component of following weight ratio:
1 part of toyokamycin, thunder godvine 50-70 part, gelsemium elegan 60-80 part, rhizome of Chinese monkshood 40-60 part, coptis 50-70 part, kitchen garden 40-60 part, cordate houttuynia 30-50 part, Herba Andrographitis 40-60 part, suspending agent 2-4 part.
Preferably, above-mentioned vegetables are mainly made by the component of following weight ratio with biopesticide:
1 part of toyokamycin, thunder godvine 55-65 part, gelsemium elegan 65-75 part, rhizome of Chinese monkshood 45-55 part, coptis 55-65 part, kitchen garden 45-55 part, cordate houttuynia 35-45 part, Herba Andrographitis 45-55 part, suspending agent 2.5-3.5 part.
Preferably, described suspending agent is a kind of in sodium alginate, methylcellulose, sodium carboxymethylcellulose, hydroxypropyl cellulose, gum Arabic.
The present invention also provides the preparation method of above-mentioned vegetables with biopesticide, and it is made by following steps:
(1) thunder godvine, the rhizome of Chinese monkshood, the coptis, kitchen garden, cordate houttuynia, Herba Andrographitis are cleaned, added the pure water of 6-8 times of volume, decoct 2-3 time, each 2-4 hour, merges extract, and while being concentrated into 30 ℃, relative density is 1.01-1.05, obtains water extraction liquid;
(2) gelsemium elegan is cleaned, added the absolute ethyl alcohol of 8-10 times of volume, refluxing extraction twice, each 2-4 hour, merges extract, reclaims ethanol, and while being concentrated into 30 ℃, relative density is 1.01-1.05, obtains alcohol extract;
(3) above-mentioned water extraction liquid and alcohol extract are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor filters, and adds toyokamycin and suspending agent, stirs, and obtains.
Beneficial effect: the present invention is with respect to prior art, and the vegetables biopesticide of gained, compares with general chemical pesticide, has the feature effective, toxicity is low, can be used as desirable environmentally friendly agricultural vegetables protection medicine.Meanwhile, the composite Chinese medical extract of toyokamycin, can expand sterilization and disinsection spectrum, can also promote drug effect, the more important thing is and can solve the drug-fast problem that the single agricultural chemicals of long-term use causes.
Embodiment
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1:
Prescription: 1 part of toyokamycin, 50 parts of thunder godvines, 60 parts of gelsemium elegan, 40 parts of the rhizome of Chinese monkshoods, 50 parts of the coptiss, 40 parts of kitchen gardens, 30 parts of cordate houttuynias, 40 parts of Herba Andrographitis, 2 parts of sodium alginates.
The preparation method of biopesticide for above-mentioned vegetables, it comprises the steps:
(1) thunder godvine, the rhizome of Chinese monkshood, the coptis, kitchen garden, cordate houttuynia, Herba Andrographitis are cleaned, added the pure water of 6 times of volumes, decoct 2 times, each 2 hours, merge extract, while being concentrated into 30 ℃, relative density is 1.01, obtains water extraction liquid;
(2) gelsemium elegan is cleaned, added the absolute ethyl alcohol of 8 times of volumes, refluxing extraction twice, each 2 hours, merge extract, reclaim ethanol, while being concentrated into 30 ℃, relative density is 1.01, obtains alcohol extract;
(3) above-mentioned water extraction liquid and alcohol extract are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor filters, and adds toyokamycin and sodium alginate, stirs, and obtains.
Embodiment 2:
Prescription: 1 part of toyokamycin, 70 parts of thunder godvines, 80 parts of gelsemium elegan, 60 parts of the rhizome of Chinese monkshoods, 70 parts of the coptiss, 60 parts of kitchen gardens, 50 parts of cordate houttuynias, 60 parts of Herba Andrographitis, 4 parts of methylcellulose.
The preparation method of biopesticide for above-mentioned vegetables, it comprises the steps:
(1) thunder godvine, the rhizome of Chinese monkshood, the coptis, kitchen garden, cordate houttuynia, Herba Andrographitis are cleaned, added the pure water of 8 times of volumes, decoct 3 times, each 4 hours, merge extract, while being concentrated into 30 ℃, relative density is 1.05, obtains water extraction liquid;
(2) gelsemium elegan is cleaned, added the absolute ethyl alcohol of 10 times of volumes, refluxing extraction twice, each 4 hours, merge extract, reclaim ethanol, while being concentrated into 30 ℃, relative density is 1.05, obtains alcohol extract;
(3) above-mentioned water extraction liquid and alcohol extract are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor filters, and adds toyokamycin and methylcellulose, stirs, and obtains.
Embodiment 3:
Prescription: 1 part of toyokamycin, 60 parts of thunder godvines, 70 parts of gelsemium elegan, 50 parts of the rhizome of Chinese monkshoods, 60 parts of the coptiss, 50 parts of kitchen gardens, 40 parts of cordate houttuynias, 50 parts of Herba Andrographitis, 3 parts of sodium carboxymethylcelluloses.
The preparation method of biopesticide for above-mentioned vegetables, it comprises the steps:
(1) thunder godvine, the rhizome of Chinese monkshood, the coptis, kitchen garden, cordate houttuynia, Herba Andrographitis are cleaned, added the pure water of 7 times of volumes, decoct 2 times, each 3 hours, merge extract, while being concentrated into 30 ℃, relative density is 1.03, obtains water extraction liquid;
(2) gelsemium elegan is cleaned, added the absolute ethyl alcohol of 9 times of volumes, refluxing extraction twice, each 3 hours, merge extract, reclaim ethanol, while being concentrated into 30 ℃, relative density is 1.03, obtains alcohol extract;
(3) above-mentioned water extraction liquid and alcohol extract are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor filters, and adds toyokamycin and sodium carboxymethylcellulose, stirs, and obtains.
Embodiment 4:
Prescription: 1 part of toyokamycin, 55 parts of thunder godvines, 65 parts of gelsemium elegan, 45 parts of the rhizome of Chinese monkshoods, 55 parts of the coptiss, 45 parts of kitchen gardens, 35 parts of cordate houttuynias, 45 parts of Herba Andrographitis, 2.5 parts of hydroxypropyl celluloses.
The preparation method of biopesticide for above-mentioned vegetables, it comprises the steps:
(1) thunder godvine, the rhizome of Chinese monkshood, the coptis, kitchen garden, cordate houttuynia, Herba Andrographitis are cleaned, added the pure water of 6 times of volumes, decoct 2 times, each 2 hours, merge extract, while being concentrated into 30 ℃, relative density is 1.02, obtains water extraction liquid;
(2) gelsemium elegan is cleaned, added the absolute ethyl alcohol of 8 times of volumes, refluxing extraction twice, each 2 hours, merge extract, reclaim ethanol, while being concentrated into 30 ℃, relative density is 1.02, obtains alcohol extract;
(3) above-mentioned water extraction liquid and alcohol extract are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor filters, and adds toyokamycin and hydroxypropyl cellulose, stirs, and obtains.
Embodiment 5:
Prescription: 1 part of toyokamycin, 65 parts of thunder godvines, 75 parts of gelsemium elegan, 55 parts of the rhizome of Chinese monkshoods, 65 parts of the coptiss, 55 parts of kitchen gardens, 45 parts of cordate houttuynias, 55 parts of Herba Andrographitis, 3.5 parts of gum Arabics.
The preparation method of biopesticide for above-mentioned vegetables, it comprises the steps:
(1) thunder godvine, the rhizome of Chinese monkshood, the coptis, kitchen garden, cordate houttuynia, Herba Andrographitis are cleaned, added the pure water of 8 times of volumes, decoct 3 times, each 4 hours, merge extract, while being concentrated into 30 ℃, relative density is 1.04, obtains water extraction liquid;
(2) gelsemium elegan is cleaned, added the absolute ethyl alcohol of 10 times of volumes, refluxing extraction twice, each 4 hours, merge extract, reclaim ethanol, while being concentrated into 30 ℃, relative density is 1.04, obtains alcohol extract;
(3) above-mentioned water extraction liquid and alcohol extract are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor filters, and adds toyokamycin and gum Arabic, stirs, and obtains.
Experimental example
By laboratory experiment, confirm, biopesticide of the present invention, to common insects of vegetables such as diamond-back moth, imported cabbageworm, tomato moth, beetle, vegetable aphid class, cabbage caterpillars, all has certain killing action, and effect is better.Experimental example is chosen the experiment of killing to diamond-back moth worm in detail, specifically sees below.
In addition, biopesticide of the present invention has certain preventive and therapeutic effect for damping off, by indoor pot measuring its preventive and therapeutic effect to cucumber rhizoctonia rot.Experimental result demonstration, biopesticide of the present invention is significantly better than the U.S. metalaxyl wetting powder (being purchased from Shenyang emerging agriculture chemical industry agricultural chemicals Co., Ltd) of 35% good fortune for the control efficiency of cucumber rhizoctonia rot.Therefore, biopesticide of the present invention has significant control vegetables damping off effect.
Gained biopesticide of the present invention the results are shown in Table 1 to the effect experiment of diamondback moth larvae
Test is the diamond-back moth of raising in indoor continuous generation for examination worm source, and test method adopts blade infusion process;
Control group A is used simple toyokamycin solution;
Control group B is used Chinese medicine extract, and described Chinese medicine extract is by the extract of Chinese medicine of the present invention and extracting method gained.
Control group C is used the simple mixtures of above-mentioned toyokamycin solution and Chinese medicine extract.
Drug dose is desinsection routine dose;
Lethality after medicine: refer to after drug administration that, after certain hour, death toll accounts for the percentage of total test number.
The effect experiment result of table 1 gained biopesticide of the present invention to diamondback moth larvae
Note: with A control group, B control group and the comparison of C control group, * P<0.05
As seen from the above table, control group C compares with control group A, control group B, and after medicine, behind 1 day, medicine, after 3 days and medicine, 7 days lethality all do not have marked change.And the biopesticide of gained of the present invention is compared with control group A, B and C, after medicine, behind 1 day, medicine, after 3 days and medicine, 7 days lethality all obviously improve, and illustrate that biopesticide insecticidal effect of the present invention significantly improves.
Claims (4)
1. a vegetables biopesticide, is characterized in that: it is mainly made by the component of following weight ratio:
1 part of toyokamycin, thunder godvine 50-70 part, gelsemium elegan 60-80 part, rhizome of Chinese monkshood 40-60 part, coptis 50-70 part,
Kitchen garden 40-60 part, cordate houttuynia 30-50 part, Herba Andrographitis 40-60 part, suspending agent 2-4 part.
2. a kind of vegetables biopesticide according to claim 1, is characterized in that: it is mainly made by the component of following weight ratio:
1 part of toyokamycin, thunder godvine 55-65 part, gelsemium elegan 65-75 part, rhizome of Chinese monkshood 45-55 part, coptis 55-65 part,
Kitchen garden 45-55 part, cordate houttuynia 35-45 part, Herba Andrographitis 45-55 part, suspending agent 2.5-3.5 part.
3. a kind of vegetables biopesticide according to claim 1, is characterized in that: described suspending agent is a kind of in sodium alginate, methylcellulose, sodium carboxymethylcellulose, hydroxypropyl cellulose, gum Arabic.
4. the preparation method of biopesticide for the vegetables described in claim 1-3 any one, is characterized in that: it is made by following steps:
(1) thunder godvine, the rhizome of Chinese monkshood, the coptis, kitchen garden, cordate houttuynia, Herba Andrographitis are cleaned, added the pure water of 6-8 times of volume, decoct 2-3 time, each 2-4 hour, merges extract, and while being concentrated into 30 ℃, relative density is 1.01-1.05, obtains water extraction liquid;
(2) gelsemium elegan is cleaned, added the absolute ethyl alcohol of 8-10 times of volume, refluxing extraction twice, each 2-4 hour, merges extract, reclaims ethanol, and while being concentrated into 30 ℃, relative density is 1.01-1.05, obtains alcohol extract;
(3) above-mentioned water extraction liquid and alcohol extract are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor filters, and adds toyokamycin and suspending agent, stirs, and obtains.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104286058A (en) * | 2014-09-24 | 2015-01-21 | 湖南农业大学 | Biopesticide bactericide prepared from Chinese herbs and use of biopesticide bactericide |
| CN104522060A (en) * | 2014-12-31 | 2015-04-22 | 江苏健神生物农化有限公司 | Environment-friendly plant pesticide and preparation method thereof |
| CN104920482A (en) * | 2015-05-21 | 2015-09-23 | 秦盼盼 | Plant source pesticide and preparation method thereof |
| CN105248488A (en) * | 2015-09-30 | 2016-01-20 | 界首市保华种植专业合作社 | Biological insecticide containing brucea javanica |
| CN105557778A (en) * | 2016-02-17 | 2016-05-11 | 广西大学 | Pesticide for killing pests and preparation method thereof |
| CN105875672A (en) * | 2016-04-22 | 2016-08-24 | 固镇县绿禾家庭农场 | Pollution-free celery disease and pest control and prevention agent |
| CN106719933A (en) * | 2016-11-18 | 2017-05-31 | 安徽禾泉农庄生态农业有限公司 | A kind of medicament for preventing and treating toad grass hang spring worm |
| CN107183081A (en) * | 2017-06-14 | 2017-09-22 | 成都璐城科技有限公司 | It is a kind of for biological pesticide of tomato and preparation method thereof |
| CN107232239A (en) * | 2017-06-08 | 2017-10-10 | 合肥龙滨化工科技有限公司 | A kind of fruits and vegetables biological pesticide composite |
| CN107996627A (en) * | 2017-11-24 | 2018-05-08 | 湖州织里创塑塑料科技有限公司 | The preparation method of textile thimerosal |
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| CN107996627A (en) * | 2017-11-24 | 2018-05-08 | 湖州织里创塑塑料科技有限公司 | The preparation method of textile thimerosal |
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