Summary of the invention
Technical problem to be solved by this invention is to provide a kind of novel bio-organic fertilizer and preparation method thereof, and it has safe, pollution-free, to improve crop quality, increase crop yield, soil protection, minimizing chemical fertilizer and agricultural chemicals usage quantity distinguishing feature.
For solving the problems of the technologies described above, the invention provides a kind of novel bio-organic fertilizer, its raw material comprises sawdust, cow dung, chicken manure, domestic refuse, stearothermophilus ground bacillus additive, plant lactobacillus additive, Chinese medicine additive, sea-tangle.
Wherein, in described bio-organic fertilizer, the weight part of each raw material is respectively sawdust 50 weight part~70 weight parts, cow dung 30 weight part~50 weight parts, chicken manure 80 weight part~100 weight parts, domestic refuse 100 weight part~120 weight parts, stearothermophilus ground bacillus additive 1 weight part~3 weight part, plant lactobacillus additive 1 weight part~3 weight part, sea-tangle 5 weight part~8 weight parts, Chinese medicine additive 15 weight part~25 weight parts.
Wherein, the preparation method of described plant lactobacillus additive is specially:
The first step activates plant lactobacillus after 24h at 32~36 ℃, is inoculated in enrichment culture liquid, 32~36 ℃ of temperature, under rotating speed 220~250rpm condition, cultivates 24h;
Second step, then by the bacterial classification after enrichment by volume mark 2~5% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, culture condition is: 32~36 ℃ of temperature, rotating speed 170~200rpm, Ventilation Rate 0.8~1.0L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h~24h, last natural air drying or 30 ℃ of oven dry, obtain solid fungicide, and the bacteria containing amount in solid fungicide is 10
10-11cfu/g.
Wherein, the preparation method of described stearothermophilus ground bacillus additive is specially:
The first step activates stearothermophilus ground bacillus after 24h at 30~34 ℃, is inoculated in enrichment culture liquid, 30~34 ℃ of temperature, under rotating speed 200~230rpm condition, cultivates 24h;
Second step, then by the bacterial classification after enrichment by volume mark 2~5% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, culture condition is: 30~34 ℃ of temperature, rotating speed 170~200rpm, Ventilation Rate 1.0~1.2L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h~24h, last natural air drying or 30 ℃ of oven dry, obtain solid fungicide, and the bacteria containing amount in solid fungicide is 10
10-11cfu/g.
Wherein, described Chinese medicine additive composition comprise that Cortex Meliae, Fructus Cnidii, Semen Pharbitidis, pivot hold, kuh-seng, Simpleleaf Shrub Chastetree Fruit, tarragon, sweet wormwood, dried lacquer, Spine of Chinese Honeylocust, Chinese prickly ash, JapaneseFloweringFernRhizome, Herba Eupatorii Odorati and Changshan.
Wherein, in described Chinese medicine additive composition, the weight part of each raw material is respectively Cortex Meliae 40 weight part~50 weight parts, Fructus Cnidii 30 weight part~40 weight parts, Semen Pharbitidis's 10 weight part~20 weight parts, pivot holds 10 weight part~20 weight parts, kuh-seng 20 weight part~30 weight parts, Simpleleaf Shrub Chastetree Fruit 10 weight part~20 weight parts, tarragon 10 weight part~20 weight parts, sweet wormwood 10 weight part~20 weight parts, dried lacquer 10 weight part~20 weight parts, Spine of Chinese Honeylocust 20 weight part~30 weight parts, Chinese prickly ash 10 weight part~20 weight parts, JapaneseFloweringFernRhizome 10 weight part~20 weight parts, Herba Eupatorii Odorati 10 weight part~20 weight parts and Changshan 10 weight part~20 weight parts.
The present invention also provides the preparation method of above-mentioned bio-organic fertilizer, is specially:
The first step, is mixed in proportion sawdust, cow dung, chicken manure, domestic refuse, Chinese medicine additive and sea-tangle, sends in stirrer and stirs, and regulates water ratio in 55-60% scope;
Second step, by regulated water ratio mixture put into fermenter, evenly add stearothermophilus ground bacillus additive and plant lactobacillus additive simultaneously, throwing is turned in mechanize, with opening material, increase amount of oxygen, one time fermentation requirement 10~15 days, fermentation top temperature must reach 60~70 ℃ of degree.Secondary Fermentation needs 5~7 days micro-aerobe fermentations, and temperature is controlled at 48-52 ℃;
The 3rd step, dries the material fermenting through fluidized-bed, temperature is controlled at 80 ℃ below degree, and water content is controlled in 10~15% scopes, pulverizes and sieves, and fineness reaches 60~80 orders, obtains bio-organic fertilizer.
Beneficial effect of the present invention:
That bio-organic fertilizer provided by the invention has is safe, pollution-free, improve crop quality, increase the distinguishing feature of crop yield, soil protection, minimizing chemical fertilizer and agricultural chemicals usage quantity.
Embodiment
The invention provides a kind of novel bio-organic fertilizer, its raw material comprises sawdust, cow dung, chicken manure, domestic refuse, stearothermophilus ground bacillus additive, plant lactobacillus additive, Chinese medicine additive, sea-tangle.
The weight part of each raw material is respectively sawdust 50 weight part~70 weight parts, cow dung 30 weight part~50 weight parts, chicken manure 80 weight part~100 weight parts, domestic refuse 100 weight part~120 weight parts, stearothermophilus ground bacillus additive 1 weight part~3 weight part, plant lactobacillus (Lactobacillus Plantarum) additive 1 weight part~3 weight part, sea-tangle 5 weight part~8 weight parts, Chinese medicine additive 15 weight part~25 weight parts.
Further, the weight part of each raw material is respectively sawdust 60 weight parts, cow dung 40 weight parts, chicken manure 90 weight parts, domestic refuse 110 weight parts, stearothermophilus ground bacillus additive 2 weight parts, plant lactobacillus (Lactobacillus Plantarum) additive 2 weight parts, sea-tangle 6 weight parts, Chinese medicine additive 20 weight parts.
Further preferably, described bio-organic fertilizer only consists of above-mentioned raw materials.
The preparation method of described plant lactobacillus additive is specially:
The first step activates plant lactobacillus after 24h at 32~36 ℃, is inoculated in enrichment culture liquid, 32~36 ℃ of temperature, under rotating speed 220~250rpm condition, cultivates 24h;
Second step, then by the bacterial classification after enrichment by volume mark 2~5% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, culture condition is: 32~36 ℃ of temperature, rotating speed 170~200rpm, Ventilation Rate 0.8~1.0L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h~24h, last natural air drying or 30 ℃ of oven dry, obtain solid fungicide, and the bacteria containing amount in solid fungicide is 10
10-11cfu/g.
Described enrichment culture liquid composition is sodium-chlor 5.0~6.0g, peptone 5.0~6.0g, beef powder 2.5~3.0g, distilled water 1000mL, pH7.0~7.2.
Described fermentation culture composition is: glucose 45g~50g, magnesium sulfate 10g~20g, potassium primary phosphate 10g~30g, dipotassium hydrogen phosphate 10g~50g, and distilled water 1000mL, regulating pH value is 7.0~7.2.
The preparation method of described stearothermophilus ground bacillus additive is specially:
The first step activates stearothermophilus ground bacillus after 24h at 30~34 ℃, is inoculated in enrichment culture liquid, 30~34 ℃ of temperature, under rotating speed 200~230rpm condition, cultivates 24h;
Second step, then by the bacterial classification after enrichment by volume mark 2~5% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, culture condition is: 30~34 ℃ of temperature, rotating speed 170~200rpm, Ventilation Rate 1.0~1.2L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 20h~24h, last natural air drying or 30 ℃ of oven dry, obtain solid fungicide, and the bacteria containing amount in solid fungicide is 10
10-11cfu/g.
Described enrichment culture liquid composition is sodium-chlor 5.0~6.0g, peptone 5.0~6.0g, beef powder 2.5~3.0g, distilled water 1000mL, pH7.0~7.2.
Described fermentation culture composition is: glucose 40~45g, ammonium nitrite 6.0~7.0g, dipotassium hydrogen phosphate 13.0~14.0g, SODIUM PHOSPHATE, MONOBASIC 2.0~3.0g, magnesium sulfate 0.5~0.6g, manganous sulfate 0.03~0.04g, yeast extract paste 0.5~0.6g, distilled water 1000mL, pH7.0~7.2.
Described domestic refuse refers to food blanking and the food residues of catering trade.
In described organic fertilizer, pass through to add Chinese medicine additive composition, utilize the germicidal action of traditional Chinese medicine ingredients, thereby guarantee growing up healthy and sound of farm crop, described Chinese medicine additive composition comprises that Cortex Meliae, Fructus Cnidii, Semen Pharbitidis, pivot hold, kuh-seng, Simpleleaf Shrub Chastetree Fruit, tarragon, sweet wormwood, dried lacquer, Spine of Chinese Honeylocust, Chinese prickly ash, JapaneseFloweringFernRhizome, Herba Eupatorii Odorati and Changshan.
Further, described Chinese medicine additive preferably only consists of above-mentioned raw materials.
In described Chinese medicine additive composition, the weight part of each raw material is respectively Cortex Meliae 40 weight part~50 weight parts, Fructus Cnidii 30 weight part~40 weight parts, Semen Pharbitidis's 10 weight part~20 weight parts, pivot holds 10 weight part~20 weight parts, kuh-seng 20 weight part~30 weight parts, Simpleleaf Shrub Chastetree Fruit 10 weight part~20 weight parts, tarragon 10 weight part~20 weight parts, sweet wormwood 10 weight part~20 weight parts, dried lacquer 10 weight part~20 weight parts, Spine of Chinese Honeylocust 20 weight part~30 weight parts, Chinese prickly ash 10 weight part~20 weight parts, JapaneseFloweringFernRhizome 10 weight part~20 weight parts, Herba Eupatorii Odorati 10 weight part~20 weight parts and Changshan 10 weight part~20 weight parts.
In described Chinese medicine additive composition, the weight part of each raw material is respectively Cortex Meliae 45 weight parts, Fructus Cnidii 35 weight parts, Semen Pharbitidis's 15 weight part, pivots and holds 15 weight parts, kuh-seng 25 weight parts, Simpleleaf Shrub Chastetree Fruit 15 weight parts, tarragon 15 weight parts, sweet wormwood 15 weight parts, dried lacquer 15 weight parts, Spine of Chinese Honeylocust 25 weight parts, Chinese prickly ash 15 weight parts, JapaneseFloweringFernRhizome 15 weight parts, Herba Eupatorii Odorati 15 weight parts and Changshan 15 weight parts.
In described Chinese medicine, the pharmacology of each raw material is as follows:
Cortex Meliae: latin name Meliae Cortex, bitter, cold in nature, return liver, spleen, stomach warp, there is desinsection, treat tinea, for ascariasis, oxyuriasis, the cards such as abdominal pain due to worm stagnation.
Fructus Cnidii: taste is pungent, bitter, warm in nature, enters kidney channel, the effect of there is warming the kidney to invigorate YANG, removing dampness and killing parasites, dispellingging wind and arresting itching, the impotence in male causing for kidney yang deficiency, woman Gong Leng are infertile, under cold-dampness band or the disease such as waist knee crymodynia.
Semen Pharbitidis: latin name Semen Pharbitidis, cold in nature, bitter, returns lung channel, kidney channel, large intestine channel, has removing fluid-retention by purgation, lower gas dissolving phlegm, the effect except long-pending desinsection, cures mainly oedema ascites, beriberi turgor, phlegm and retained fluid is breathed with cough, complete works of constipated, eats stagnant malnutrition due to parasitic infestation, swollen ulcer drug.
Pivot holds: latin name Polygoni Avicularis Herba, and bitter, cold nature, enters urinary bladder channel, has relieving stranguria by diuresis, the effect of killing parasites to relieve itching, for stranguria, especially sand drenches, urolithiasis, dysuria, jaundice due to damp-heat, rushes down dysentery, is with inferior Zheng , pivot to hold and has significant diuretic properties.
Kuh-seng: latin name Sophorae Flavescentis Radix, bitter, cold in nature, enter the heart, stomach, large intestine, urinary bladder channel, there is the effect of heat-clearing and damp-drying drug, wind dispelling insecticide, be mainly used in damp-heat dysentery, jaundice, stranguria, kuh-seng has obvious restraining effect to dysentery bacterium, intestinal bacteria, Bacillus proteus, beta streptococcus and streptococcus aureus.
Simpleleaf Shrub Chastetree Fruit: taste is pungent, bitter, cold nature, returns bladder, liver, stomach warp, has dispelling wind and heat pathogens, and the clear sharp head's effect cures mainly common cold due to wind-heat headache, gingivitis, the many tear of hot eyes, order is secretly not clear, the card such as have a dizzy spell.
Tarragon: taste is pungent, bitter, warm in nature, returns liver,spleen,kidney warp, has dispersing cold for relieving pain, and the effect of warming the meridian for stopping bleeding, cures mainly haematemesis; Bleeding from five sense organs or subcutaneous tissue; Painstaking blood; Have blood in stool; Uterine bleeding; Gestation hemorrhage; Menoxenia; Dysmenorrhoea; Threatened abortion; Trusted subordinate's crymodynia; The protracted dysentery of having loose bowels; Cramp caused by acute vomiting and diarrhea; Under band; Eczema; Mange; Hemorrhoid; The cards such as large carbuncle.
Sweet wormwood: latin name Artemisiae Annuae Herba, bitter, pungent, cold in nature, return liver, gallbladder channel, have abnormal heat clearly thoroughly, cool blood is except steaming, and except steaming, the effect of preventing malaria, cures mainly summer-heat evil heating, fever due to yin deficiency, night fever abating at dawn, hectic fever due to yin labor heat, malaria fever and chills, the cards such as jaundice due to damp-heat.
Dried lacquer: taste is pungent, warm in nature, return liver, the spleen channel, there is dissipating blood stasis to promote menstruationg, the effect of eliminating stagnated food to destroy intestinal worms, for hemostasis through closing, the cards such as abdominal pain due to worm stagnation.
Spine of Chinese Honeylocust: taste is pungent, warm in nature, return lung channel, cure mainly swollen ulcer drug, scrofula, sore rash stubborn dermatitis, puerperal hypogalactia, retention of placenta, the cards such as pestilence wind.
Chinese prickly ash: taste is pungent, warm in nature, return spleen, stomach, kidney channel, there is warming middle-JIAO to relieve pain, the effect of killing parasites to relieve itching.For coldness and pain in the epigastrium, vomiting is had loose bowels, abdominal pain due to worm stagnation, ascariasis, the cards such as eczema itch.
JapaneseFloweringFernRhizome: bitter, cold nature, returns spleen, and stomach warp has clearing heat and detoxicatingly, dissolving stasis and hemostasis, and the effect of desinsection, for influenza; Epidemic meningitis; Encephalitis parotitis; Carbuncle pyogenic infections from tumour or sore; Measles; Varicella; Dysentery; Spit blood; Bleeding from five sense organs or subcutaneous tissue; Have blood in stool; Uterine bleeding; Under band; Pinworm; The cards such as tapeworm.
Herba Eupatorii Odorati: taste is micro-pungent, warm in nature, has the effect of the loose stasis of blood, removing toxic substances, desinsection, hemostasis, cures mainly treating swelling and pain by traumatic injury, sore swollen toxin, paddy field dermatitis, the cards such as traumatic hemorrhage.
Changshan: latin name Dichroae Radix, bitter, pungent, cold in nature, return lung, liver, the heart channel of Hang-Shaoyin, there is the effect of vomiting phlegm and saliva by emesis, cure mainly phlegm and retained fluid and stop gathering, the cards such as chest diaphragm ruffian plug.
The present invention also provides the preparation method of above-mentioned bio-organic fertilizer, is specially:
The first step, is mixed in proportion sawdust, cow dung, chicken manure, domestic refuse, Chinese medicine additive and sea-tangle, sends in stirrer and stirs, and regulates water ratio in 55-60% scope;
Second step, by having regulated the mixture of water ratio, put into fermenter, evenly add stearothermophilus ground bacillus additive and plant lactobacillus additive simultaneously, throwing is turned in mechanize, with opening material, increase amount of oxygen, one time fermentation requirement 10~15 days, fermentation top temperature must reach 60~70 ℃ of degree.Secondary Fermentation needs 5~7 days micro-aerobe fermentations, and temperature is controlled at 48~52 ℃;
The 3rd step, dries the material fermenting through fluidized-bed, temperature is controlled at 80 ℃ below degree, and water content is controlled in 10~15% scopes, pulverizes and sieves, and fineness reaches 60~80 orders, obtains bio-organic fertilizer.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how utilisation technology means solve technical problem whereby, and the implementation procedure of reaching technique effect can fully understand and implement according to this.
The preparation of embodiment 1 plant lactobacillus additive
Plant lactobacillus (Shanghai enzyme joins biological Consumables Co., Ltd. to be provided) is activated after 24h at 33 ℃, be inoculated in enrichment culture liquid, described enrichment culture liquid composition is sodium-chlor 5.0g, peptone 5.0g, beef powder 2.5g, distilled water 1000mL, pH7.0, 33 ℃ of temperature, under rotating speed 250rpm condition, cultivate 24h, then by the bacterial classification after enrichment by volume mark 3% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, described fermentation culture composition is: glucose 50g, magnesium sulfate 20g, potassium primary phosphate 20g, dipotassium hydrogen phosphate 30g, distilled water 1000mL, regulating pH value is 7.0, culture condition is: 33 ℃ of temperature, rotating speed 200rpm, Ventilation Rate 1.0L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 24h, finally 30 ℃ of oven dry, obtain solid fungicide, bacteria containing amount in solid fungicide is 10
10-11cfu/g.
The preparation of embodiment 2 stearothermophilus ground bacillus
Stearothermophilus ground bacillus (Shanghai enzyme joins biological Consumables Co., Ltd. to be provided) is activated after 24h at 34 ℃, be inoculated in enrichment culture liquid, described enrichment culture liquid composition is sodium-chlor 6.0g, peptone 6.0g, beef powder 3.0g, distilled water 1000mL, pH7.0, 34 ℃ of temperature, under rotating speed 230rpm condition, cultivate 24h, then by the bacterial classification after enrichment by volume mark 5% be inoculated in and in aerobic fermentation nutrient solution, carry out aerobic fermentation cultivation, described fermentation culture composition is: glucose 45g, ammonium nitrite 7.0g, dipotassium hydrogen phosphate 14.0g, SODIUM PHOSPHATE, MONOBASIC 3.0g, magnesium sulfate 0.6g, manganous sulfate 0.04g, yeast extract paste 0.6g, distilled water 1000mL, pH7.0, culture condition is: 34 ℃ of temperature, rotating speed 200rpm, Ventilation Rate 1.2L ∕ Lmin, the air passing into is crossed 0.45 μ m filter membrane and is carried out filtration sterilization, aerobic fermentation incubation time is 24h, finally 30 ℃ of oven dry, obtain solid fungicide, bacteria containing amount in solid fungicide is 10
10-11cfu/g.
The preparation of embodiment 3 bio-organic fertilizers 1
By Cortex Meliae 450g, Fructus Cnidii 350g, Semen Pharbitidis 150g, pivot holds 150g, kuh-seng 250g, Simpleleaf Shrub Chastetree Fruit 150g, tarragon 150g, sweet wormwood 150g, dried lacquer 150g, Spine of Chinese Honeylocust 250g, Chinese prickly ash 150g, JapaneseFloweringFernRhizome 150g, Herba Eupatorii Odorati 150g and Changshan 150g send in stirrer and are uniformly mixed, by sawdust 6kg, cow dung 4kg, chicken manure 9kg, domestic refuse (leftovers leftovers) 11kg, the Traditional Chinese drug mixture 2kg preparing above and sea-tangle 0.6kg mix, send in stirrer and stir, regulate water ratio in 55% scope, by regulated water ratio mixture put into fermenter, evenly add the stearothermophilus ground bacillus additive 0.2kg of embodiment 2 preparations and the plant lactobacillus additive 0.2kg of embodiment 1 preparation simultaneously, throwing is turned in mechanize, with opening material, increase amount of oxygen, one time fermentation requirement 12 days, fermentation top temperature must reach 60 ℃ of degree.Secondary Fermentation needs 7 days micro-aerobe fermentations, and temperature is controlled at 50 ℃, and the material fermenting is dried through fluidized-bed, and temperature is controlled at 80 ℃ below degree, and water content is controlled in 15% scope, pulverizes and sieves, and fineness reaches 80 orders, obtains bio-organic fertilizer 1.
Comparative example 1 bio-organic fertilizer 2
Sawdust 6kg, cow dung 4kg, chicken manure 9kg, domestic refuse (leftovers leftovers) 11kg and sea-tangle 0.6kg are mixed, send in stirrer and stir, regulate water ratio in 55% scope, by regulated water ratio mixture put into fermenter, evenly add the stearothermophilus ground bacillus additive 0.2kg of embodiment 2 preparations and the plant lactobacillus additive 0.2kg of embodiment 1 preparation simultaneously, throwing is turned in mechanize, with opening material, increase amount of oxygen, one time fermentation requirement 12 days, fermentation top temperature must reach 60 ℃ of degree.Secondary Fermentation needs 7 days micro-aerobe fermentations, and temperature is controlled at 50 ℃, and the material fermenting is dried through fluidized-bed, and temperature is controlled at 80 ℃ below degree, and water content is controlled in 15% scope, pulverizes and sieves, and fineness reaches 80 orders, obtains bio-organic fertilizer 2.
Comparative example 2 bio-organic fertilizers 3
By Cortex Meliae 450g, Fructus Cnidii 350g, Semen Pharbitidis 150g, pivot holds 150g, kuh-seng 250g, Simpleleaf Shrub Chastetree Fruit 150g, tarragon 150g, sweet wormwood 150g, dried lacquer 150g, Spine of Chinese Honeylocust 250g, Chinese prickly ash 150g, JapaneseFloweringFernRhizome 150g, Herba Eupatorii Odorati 150g and Changshan 150g send in stirrer and are uniformly mixed, by sawdust 6kg, cow dung 4kg, chicken manure 9kg, domestic refuse (leftovers leftovers) 11kg, the Traditional Chinese drug mixture 2kg preparing above and sea-tangle 0.6kg mix, send in stirrer and stir, regulate water ratio in 55% scope, by regulated water ratio mixture put into fermenter, plant lactobacillus additive 0.2kg simultaneously that evenly add embodiment 1 preparation, throwing is turned in mechanize, with opening material, increase amount of oxygen, one time fermentation requirement 12 days, fermentation top temperature must reach 60 ℃ of degree.Secondary Fermentation needs 7 days micro-aerobe fermentations, and temperature is controlled at 50 ℃, and the material fermenting is dried through fluidized-bed, and temperature is controlled at 80 ℃ below degree, and water content is controlled in 15% scope, pulverizes and sieves, and fineness reaches 80 orders, obtains bio-organic fertilizer 3.
Comparative example 3 bio-organic fertilizers 4
By Cortex Meliae 450g, Fructus Cnidii 350g, Semen Pharbitidis 150g, pivot holds 150g, kuh-seng 250g, Simpleleaf Shrub Chastetree Fruit 150g, tarragon 150g, sweet wormwood 150g, dried lacquer 150g, Spine of Chinese Honeylocust 250g, Chinese prickly ash 150g, JapaneseFloweringFernRhizome 150g, Herba Eupatorii Odorati 150g and Changshan 150g send in stirrer and are uniformly mixed, by sawdust 6kg, cow dung 4kg, chicken manure 9kg, domestic refuse (leftovers leftovers) 11kg, the Traditional Chinese drug mixture 2kg preparing above and sea-tangle 0.6kg mix, send in stirrer and stir, regulate water ratio in 55% scope, by regulated water ratio mixture put into fermenter, stearothermophilus ground bacillus additive 0.2kg simultaneously that evenly add embodiment 2 preparations, throwing is turned in mechanize, with opening material, increase amount of oxygen, one time fermentation requirement 12 days, fermentation top temperature must reach 60 ℃ of degree.Secondary Fermentation needs 7 days micro-aerobe fermentations, and temperature is controlled at 50 ℃, and the material fermenting is dried through fluidized-bed, and temperature is controlled at 80 ℃ below degree, and water content is controlled in 15% scope, pulverizes and sieves, and fineness reaches 80 orders, obtains bio-organic fertilizer 4.
The effect of bio-organic fertilizer provided by the invention in actual proportion of crop planting is as follows:
In areas such as Linyi, Shandong Province, Jinan, Qingdao, test.
Experimental group and control group are set 5 groups altogether.For examination soil: moisture soil, quality loam, physical features is smooth, and water flowing, water conservancy condition are better, fertile soil; For trying object: paddy rice.
Experimental group and control group 1 to control group 3 is used the microorganism organic fertilizer of the production that embodiment 1 and comparative example 1 to comparative example 3 provide, when crop is broadcast preplanting treatment, be evenly sprinkled into every mu of consumption 40kg, crop is after planting used during one and a half months for the second time, every mu of consumption 30kg.
Control group 4: use commercially available compound manure Waterville (Shandong Sheng Gaofei industry company limited provides), use mineral manure 80kg for every mu, divide and use for three times, every minor tick one month.
Other management conditions are all consistent.
Experimental group and control group all carry out 3 groups of Experimental Comparisons, are applied to paddy rice, and experimental result sees the following form 1.
Table 1 paddy rice comparative test result
Project |
Experimental group 1 |
Control group 1 |
Control group 2 |
Control group 3 |
Control group 4 |
Average yield per mu kg |
723 |
587 |
607 |
611 |
556 |
Every mu of units increased in production kg |
? |
136 |
116 |
112 |
167 |
Stimulation ratio % |
? |
23.2% |
19.1% |
18.3% |
30.0% |
Note: every mu of units increased in production refers to the output that experimental group increases with respect to control group.
As can be seen from Table 1, adopt bio-organic fertilizer of the present invention to increase significantly with respect to control group tool to increasing production of rice.
The impact of experimental group bio-organic fertilizer on soil organic matter content, the results are shown in Table 2.
Table 2 bio-organic fertilizer affects soil organic content
Rate of fertilizer application/(kg/ mu) |
20 |
40 |
60 |
80 |
Soil organic matter content/(%) |
1.83 |
1.80 |
1.79 |
1.81 |
Be with organic quantity/(the kg/ mu) that buries |
10 |
15 |
20 |
25 |
Soil organism increasing amount/(kg/ mu) |
120 |
140 |
120 |
110 |
All above-mentioned these intellecture properties of primary enforcement, do not set restriction this product innovation of other forms of enforcement and/or novel method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on product innovation of the present invention.
The above, be only preferred embodiment of the present invention, is not the present invention to be done to the restriction of other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not depart from, any simple modification, equivalent variations and the remodeling above embodiment done according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.