CN103562375A - 用于产生异丁酸的细胞和方法 - Google Patents

用于产生异丁酸的细胞和方法 Download PDF

Info

Publication number
CN103562375A
CN103562375A CN201280017985.8A CN201280017985A CN103562375A CN 103562375 A CN103562375 A CN 103562375A CN 201280017985 A CN201280017985 A CN 201280017985A CN 103562375 A CN103562375 A CN 103562375A
Authority
CN
China
Prior art keywords
cell
genetically modified
recombinant cell
polypeptide
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201280017985.8A
Other languages
English (en)
Chinese (zh)
Inventor
K.张
M.熊
A.P.伍德拉夫
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UNVIVERSITY OF MINNESOTA
Original Assignee
UNVIVERSITY OF MINNESOTA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UNVIVERSITY OF MINNESOTA filed Critical UNVIVERSITY OF MINNESOTA
Publication of CN103562375A publication Critical patent/CN103562375A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201280017985.8A 2011-02-11 2012-02-10 用于产生异丁酸的细胞和方法 Pending CN103562375A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161441939P 2011-02-11 2011-02-11
US61/441,939 2011-02-11
PCT/US2012/024640 WO2012109534A2 (en) 2011-02-11 2012-02-10 Cells and methods for producing isobutyric acid

Publications (1)

Publication Number Publication Date
CN103562375A true CN103562375A (zh) 2014-02-05

Family

ID=45722726

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280017985.8A Pending CN103562375A (zh) 2011-02-11 2012-02-10 用于产生异丁酸的细胞和方法

Country Status (8)

Country Link
US (1) US20140065697A1 (https=)
EP (1) EP2673355A2 (https=)
JP (1) JP2014506466A (https=)
KR (1) KR20140061303A (https=)
CN (1) CN103562375A (https=)
AU (1) AU2012214255A1 (https=)
SG (1) SG192706A1 (https=)
WO (1) WO2012109534A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105296410A (zh) * 2015-10-27 2016-02-03 中国科学院青岛生物能源与过程研究所 一种利用缬氨酸途径合成丙烷的大肠杆菌及其构建方法

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013055667A2 (en) * 2011-10-10 2013-04-18 Regents Of The University Of Minnesota Biocatalysis cells and methods
CN104685059A (zh) * 2012-05-29 2015-06-03 明尼苏达大学评议会 生物合成途径、重组细胞和方法
WO2013192237A1 (en) * 2012-06-18 2013-12-27 The Regents Of The University Of California Escherichia coli engineered for isobutyraldehyde production
WO2014028642A1 (en) 2012-08-17 2014-02-20 Easel Biotechnologies, Llc Two stage production of higher alcohols
CN105705650A (zh) * 2013-08-28 2016-06-22 英威达技术有限责任公司 用于生物合成异丁烯酸盐的方法
JP2017131111A (ja) 2014-06-03 2017-08-03 味の素株式会社 Ｌ−アミノ酸の製造法
WO2015191611A1 (en) * 2014-06-09 2015-12-17 The Regents Of The University Of California Bacteria engineered for conversion of ethylene to n-butanol
AU2016265751A1 (en) 2015-05-19 2017-12-14 Lucite International Uk Limited Process for the biological production of methacrylic acid and derivatives thereof
US20200308610A1 (en) 2016-03-11 2020-10-01 Aemitis, Inc. Alpha-ketoisocaproic acid and & alpha-keto-3-methylvaleric acid decarboxylases and uses thereof
WO2017194696A1 (en) 2016-05-12 2017-11-16 Danmarks Tekniske Universitet Bacterial cells with improved tolerance to isobutyric acid
US10849938B2 (en) 2017-09-13 2020-12-01 ZBiotics Company Gene expression system for probiotic microorganisms
US11680280B2 (en) 2018-01-09 2023-06-20 Lygos, Inc. Recombinant host cells and methods for the production of isobutyric acid

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4452999A (en) * 1979-12-20 1984-06-05 Rohm Gmbh Method for making isobutyric acid
WO2010017230A2 (en) * 2008-08-04 2010-02-11 The Regents Of The University Of Colorado Methods, systems and compositions related to microbial bio-production of butanol and/or isobutanol
CN101679187A (zh) * 2007-06-01 2010-03-24 赢创罗姆有限责任公司 用于制备甲基丙烯酸或甲基丙烯酸酯的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4754074A (en) 1986-12-22 1988-06-28 Amoco Corporation Preparation of dialkyl ketones from aliphatic carboxylic acids
US20090053797A1 (en) * 2005-08-19 2009-02-26 Yoichiro Shiba Genetically modified host cells and use of same for producing isoprenoid compounds
KR100780324B1 (ko) * 2006-07-28 2007-11-29 한국과학기술원 신규 순수 숙신산 생성 변이 미생물 및 이를 이용한 숙신산제조방법
EP2350299A4 (en) * 2008-10-28 2012-04-25 Univ Rice William M MICROAEROBE CULTURES FOR THE CONVERSION OF GLYCERINE IN CHEMICALS
BR112012020261B8 (pt) * 2010-02-12 2020-04-14 California Inst Of Techn saccharomyces cerevisiae recombinante e método de produção de isobutanol

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4452999A (en) * 1979-12-20 1984-06-05 Rohm Gmbh Method for making isobutyric acid
CN101679187A (zh) * 2007-06-01 2010-03-24 赢创罗姆有限责任公司 用于制备甲基丙烯酸或甲基丙烯酸酯的方法
WO2010017230A2 (en) * 2008-08-04 2010-02-11 The Regents Of The University Of Colorado Methods, systems and compositions related to microbial bio-production of butanol and/or isobutanol

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FERRANDEZ A，等: "MOLECULAR CHARACTERIZATION OF PADA, A PHENYLACETALDEHYDE DEHYDROGENASE FROM ESCHERICHIA COLI", 《FEBS LETTERS》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105296410A (zh) * 2015-10-27 2016-02-03 中国科学院青岛生物能源与过程研究所 一种利用缬氨酸途径合成丙烷的大肠杆菌及其构建方法

Also Published As

Publication number Publication date
WO2012109534A3 (en) 2012-10-04
EP2673355A2 (en) 2013-12-18
SG192706A1 (en) 2013-09-30
KR20140061303A (ko) 2014-05-21
AU2012214255A1 (en) 2013-09-12
WO2012109534A2 (en) 2012-08-16
US20140065697A1 (en) 2014-03-06
JP2014506466A (ja) 2014-03-17

Similar Documents

Publication Publication Date Title
CN103562375A (zh) 用于产生异丁酸的细胞和方法
Nielsen et al. Metabolic engineering of acetoin and meso‐2, 3‐butanediol biosynthesis in E. coli
US10006064B2 (en) Biosynthetic pathways, recombinant cells, and methods
US20120244588A1 (en) Method of producing 3-hydroxypropionic acid using malonic semialdehyde reducing pathway
US20170044551A1 (en) High yield route for the production of compounds from renewable sources
WO2010022763A1 (en) Method for the preparation of 2-hydroxy-isobutyrate
IL268816B1 (en) Preparations and methods for robust dynamic metabolic control
JP2022046736A (ja) グリコール酸および／またはグリオキシル酸の製造のための方法および微生物
JP2017534268A (ja) 有用産物の生産のための改変微生物および方法
US20250075235A1 (en) Production of ethanol with one or more co-products in yeast
MX2015004180A (es) Microorganismos recombinantes para producir acidos organicos organicos.
WO2018122388A1 (en) Conversion of methylglyoxal into hydroxyacetone using novel enzymes and applications thereof
US20130288320A1 (en) Methods and microorganisms for increasing the biological synthesis of difunctional alkanes
WO2015031504A1 (en) RECOMBINANT PATHWAY AND ORGANISMS FOR MALONYL-CoA SYNTHESIS
US12104160B2 (en) Production of 4,6-dihydroxy-2-oxo-hexanoic acid
KR20220021436A (ko) 고알칼리 조건에서 활성이 높은 신규 라이신 디카르복실라제 및 이를 이용한 카다베린 생산 방법
WO2023186037A1 (zh) 一种利用苏氨酸制备甘氨酸、乙酰辅酶a及乙酰辅酶a衍生物的方法
US20140329275A1 (en) Biocatalysis cells and methods
US20240132833A1 (en) Compositions and methods for improved malonyl-CoA biosynthesis using 2-stage dynamic metabolic control

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20140205