CN103525537A - 一种提取微生物油脂的方法 - Google Patents
一种提取微生物油脂的方法 Download PDFInfo
- Publication number
- CN103525537A CN103525537A CN201310497556.1A CN201310497556A CN103525537A CN 103525537 A CN103525537 A CN 103525537A CN 201310497556 A CN201310497556 A CN 201310497556A CN 103525537 A CN103525537 A CN 103525537A
- Authority
- CN
- China
- Prior art keywords
- extraction
- oil
- microbial oil
- circulation
- value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000000813 microbial effect Effects 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000000605 extraction Methods 0.000 claims abstract description 77
- 238000000855 fermentation Methods 0.000 claims abstract description 40
- 230000004151 fermentation Effects 0.000 claims abstract description 40
- 238000002156 mixing Methods 0.000 claims abstract description 39
- 239000002245 particle Substances 0.000 claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 238000000926 separation method Methods 0.000 claims abstract description 21
- 239000002253 acid Substances 0.000 claims abstract description 16
- 150000002978 peroxides Chemical class 0.000 claims abstract description 15
- 239000000839 emulsion Substances 0.000 claims abstract description 14
- 239000011259 mixed solution Substances 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000003960 organic solvent Substances 0.000 claims abstract description 11
- 241000894006 Bacteria Species 0.000 claims description 30
- 241000209094 Oryza Species 0.000 claims description 21
- 235000007164 Oryza sativa Nutrition 0.000 claims description 21
- 235000009566 rice Nutrition 0.000 claims description 21
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 18
- 241000907999 Mortierella alpina Species 0.000 claims description 13
- 238000011081 inoculation Methods 0.000 claims description 12
- 238000001556 precipitation Methods 0.000 claims description 12
- 108010059820 Polygalacturonase Proteins 0.000 claims description 9
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 6
- 102000035195 Peptidases Human genes 0.000 claims description 6
- 108091005804 Peptidases Proteins 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 238000013467 fragmentation Methods 0.000 claims description 6
- 238000006062 fragmentation reaction Methods 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 6
- 241000003595 Aurantiochytrium limacinum Species 0.000 claims description 4
- 108010059892 Cellulase Proteins 0.000 claims description 4
- 241000199912 Crypthecodinium cohnii Species 0.000 claims description 4
- 241000199914 Dinophyceae Species 0.000 claims description 4
- 241000306282 Umbelopsis isabellina Species 0.000 claims description 4
- 229940106157 cellulase Drugs 0.000 claims description 4
- 229940059442 hemicellulase Drugs 0.000 claims description 4
- 108010002430 hemicellulase Proteins 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 238000005265 energy consumption Methods 0.000 abstract description 3
- 230000003647 oxidation Effects 0.000 abstract description 3
- 238000007254 oxidation reaction Methods 0.000 abstract description 3
- 238000001035 drying Methods 0.000 abstract description 2
- 241001052560 Thallis Species 0.000 abstract 2
- 238000004807 desolvation Methods 0.000 abstract 1
- 230000006866 deterioration Effects 0.000 abstract 1
- 238000010565 inoculated fermentation Methods 0.000 abstract 1
- 235000012054 meals Nutrition 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 50
- 239000004519 grease Substances 0.000 description 24
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 20
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 15
- 229940114079 arachidonic acid Drugs 0.000 description 10
- 235000021342 arachidonic acid Nutrition 0.000 description 10
- DVSZKTAMJJTWFG-UHFFFAOYSA-N docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCCC=CC=CC=CC=CC=CC=CC(O)=O DVSZKTAMJJTWFG-UHFFFAOYSA-N 0.000 description 5
- MBMBGCFOFBJSGT-KUBAVDMBSA-N docosahexaenoic acid Natural products CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 3
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 3
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 3
- 206010020843 Hyperthermia Diseases 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000010981 drying operation Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000036031 hyperthermia Effects 0.000 description 2
- 235000020660 omega-3 fatty acid Nutrition 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- HXWJFEZDFPRLBG-UHFFFAOYSA-N Timnodonic acid Natural products CCCC=CC=CCC=CCC=CCC=CCCCC(O)=O HXWJFEZDFPRLBG-UHFFFAOYSA-N 0.000 description 1
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 1
- 150000001721 carbon Chemical group 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 125000005908 glyceryl ester group Chemical group 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229960004232 linoleic acid Drugs 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 229940127293 prostanoid Drugs 0.000 description 1
- 150000003814 prostanoids Chemical class 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 125000005480 straight-chain fatty acid group Chemical group 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
Abstract
Description
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310497556.1A CN103525537B (zh) | 2013-10-22 | 2013-10-22 | 一种提取微生物油脂的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310497556.1A CN103525537B (zh) | 2013-10-22 | 2013-10-22 | 一种提取微生物油脂的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103525537A true CN103525537A (zh) | 2014-01-22 |
CN103525537B CN103525537B (zh) | 2015-12-30 |
Family
ID=49927892
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310497556.1A Active CN103525537B (zh) | 2013-10-22 | 2013-10-22 | 一种提取微生物油脂的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103525537B (zh) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103642698A (zh) * | 2013-11-18 | 2014-03-19 | 嘉必优生物工程(武汉)有限公司 | 高山被孢霉突变株及其应用 |
CN103882071A (zh) * | 2014-03-14 | 2014-06-25 | 嘉必优生物工程(武汉)有限公司 | 一种微生物油及其制备方法 |
CN103981009A (zh) * | 2014-05-04 | 2014-08-13 | 厦门汇盛生物有限公司 | 一种裂殖壶菌发酵液破壁提取其胞内油脂的方法 |
CN104479862A (zh) * | 2014-12-31 | 2015-04-01 | 嘉必优生物工程(武汉)有限公司 | 一种提取微生物油脂的方法 |
CN104531342A (zh) * | 2014-12-18 | 2015-04-22 | 北京化工大学 | 一种温和高效提取微生物油脂的方法 |
CN106977394A (zh) * | 2016-12-01 | 2017-07-25 | 烟台固特丽生物科技股份有限公司 | 一种快速提取低色度花生四烯酸油脂的工艺 |
CN112553110A (zh) * | 2020-12-16 | 2021-03-26 | 中国科学院合肥物质科学研究院 | 一种菌粕液的制备方法及利用菌粕液的发酵培养基与应用 |
CN113133495A (zh) * | 2021-05-26 | 2021-07-20 | 广州优百特科技有限公司 | 一种生物油脂脂肪粉及其制备方法和应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101108997A (zh) * | 2006-07-19 | 2008-01-23 | 中国科学院大连化学物理研究所 | 一种微生物油脂的制备方法 |
CN101560440A (zh) * | 2009-06-02 | 2009-10-21 | 北京化工大学 | 一种微生物油脂的制备方法 |
CN102250974A (zh) * | 2010-05-19 | 2011-11-23 | 中国科学院大连化学物理研究所 | 一种微生物油脂的制备方法 |
CN102925280A (zh) * | 2011-08-10 | 2013-02-13 | 嘉必优生物工程(湖北)有限公司 | 一种微生物油脂的提取及精炼方法 |
-
2013
- 2013-10-22 CN CN201310497556.1A patent/CN103525537B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101108997A (zh) * | 2006-07-19 | 2008-01-23 | 中国科学院大连化学物理研究所 | 一种微生物油脂的制备方法 |
CN101560440A (zh) * | 2009-06-02 | 2009-10-21 | 北京化工大学 | 一种微生物油脂的制备方法 |
CN102250974A (zh) * | 2010-05-19 | 2011-11-23 | 中国科学院大连化学物理研究所 | 一种微生物油脂的制备方法 |
CN102925280A (zh) * | 2011-08-10 | 2013-02-13 | 嘉必优生物工程(湖北)有限公司 | 一种微生物油脂的提取及精炼方法 |
Non-Patent Citations (1)
Title |
---|
墨玉欣 等: "微生物发酵制备油脂的研究", 《可再生能源》 * |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103642698A (zh) * | 2013-11-18 | 2014-03-19 | 嘉必优生物工程(武汉)有限公司 | 高山被孢霉突变株及其应用 |
CN103642698B (zh) * | 2013-11-18 | 2015-11-04 | 嘉必优生物工程(武汉)有限公司 | 高山被孢霉突变株及其应用 |
CN103882071A (zh) * | 2014-03-14 | 2014-06-25 | 嘉必优生物工程(武汉)有限公司 | 一种微生物油及其制备方法 |
CN105925627A (zh) * | 2014-03-14 | 2016-09-07 | 嘉必优生物技术(武汉)股份有限公司 | 微生物油及其制备方法 |
CN103882071B (zh) * | 2014-03-14 | 2016-10-05 | 嘉必优生物技术(武汉)股份有限公司 | 一种微生物油及其制备方法 |
CN105925627B (zh) * | 2014-03-14 | 2019-08-13 | 嘉必优生物技术(武汉)股份有限公司 | 微生物油及其制备方法 |
CN103981009A (zh) * | 2014-05-04 | 2014-08-13 | 厦门汇盛生物有限公司 | 一种裂殖壶菌发酵液破壁提取其胞内油脂的方法 |
CN104531342A (zh) * | 2014-12-18 | 2015-04-22 | 北京化工大学 | 一种温和高效提取微生物油脂的方法 |
CN104479862A (zh) * | 2014-12-31 | 2015-04-01 | 嘉必优生物工程(武汉)有限公司 | 一种提取微生物油脂的方法 |
CN106977394A (zh) * | 2016-12-01 | 2017-07-25 | 烟台固特丽生物科技股份有限公司 | 一种快速提取低色度花生四烯酸油脂的工艺 |
CN112553110A (zh) * | 2020-12-16 | 2021-03-26 | 中国科学院合肥物质科学研究院 | 一种菌粕液的制备方法及利用菌粕液的发酵培养基与应用 |
CN113133495A (zh) * | 2021-05-26 | 2021-07-20 | 广州优百特科技有限公司 | 一种生物油脂脂肪粉及其制备方法和应用 |
Also Published As
Publication number | Publication date |
---|---|
CN103525537B (zh) | 2015-12-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103525537B (zh) | 一种提取微生物油脂的方法 | |
JP6665147B2 (ja) | 微生物細胞及び微生物油の低温殺菌方法 | |
KR101648277B1 (ko) | 미생물 오일의 분리방법 | |
KR101208304B1 (ko) | 다가불포화 지방산을 함유하는 미생물 오일의 제조방법 | |
JP2004505168A (ja) | 未精製のpufa含有油混合物の精製方法 | |
CN104479862A (zh) | 一种提取微生物油脂的方法 | |
WO2006046943A2 (en) | Methods for producing lipids by liberation from biomass | |
CN105925627B (zh) | 微生物油及其制备方法 | |
CN105713936B (zh) | 微生物油的制备方法 | |
KR20140146156A (ko) | 다가불포화 지방산을 함유하는 미생물 오일의 제조방법 | |
CN117229313A (zh) | 一种化学催化法制备磷脂型多不饱和脂肪酸及其制备方法与应用 | |
CN104513704A (zh) | 一种无腥味dha油脂的提取方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C56 | Change in the name or address of the patentee | ||
CP03 | Change of name, title or address |
Address after: 430074 Wuhan Province, East Lake New Technology Development Zone, high tech Avenue, No. 999, No. Patentee after: Limited by Share Ltd Biotechnology (Wuhan) Co., Ltd. Address before: 430223 Hubei province Wuhan city East Lake high tech Park Road No. two Guanshan international business center 3-504 Patentee before: Chia lung Biological Engineering (Wuhan) Co., Ltd. |
|
CB03 | Change of inventor or designer information |
Inventor after: Wang Zhiming Inventor after: Li Xiangyu Inventor after: Tian Yong Inventor after: Zhou Qiang Inventor after: Yang Gang Inventor after: Lu Shuhuan Inventor after: Yu Chao Inventor before: Wang Zhiming Inventor before: Tian Yong Inventor before: Zhou Qiang Inventor before: Yang Gang Inventor before: Lu Shuhuan Inventor before: Yu Chao |
|
CB03 | Change of inventor or designer information |